Questions related to Veterinary Parasitology
I need to observe the fecal egg reduction in rats that I'm studying. I need a protocol how to do it without a McMaster slide.
I am searching for a research topic and funding for masters of veterinary parasitology. I will conduct research in Nepal. do you have any suggestions?? please provide a good topic and funding for research. Thank you in advance. hashtag#research hashtag#veterinary hashtag#topic hashtag#funding hashtag#science
Hello. I found this "eggs" (or not) in Dennis, Stone & Swanson technique on equine samples. It's in 40x, 40x and 10x , respectivel. I will be glad if someone could help me to identificate this structures. Thanks!
Dear Colleagues, eggs of approx. 120×60 micrm size were freshly floated from a 6-month-old heifer's intestinal content. They do not resemble to any other eggs previously seen in our lab. What could they be? Artifacts or something of significance? Any suggestions on genera or species would be welcome. Thank you in advance!
So to summarize my research I have an OTU table of 17 cyathostomin OTUs from equine fecal samples obtained using Illumina sequencing. The data set comprises of about 3 different time courses where a different anthelmintic was used each time. A sample was obtained from each horse biweekly following the treatment. Count data was obtained when sequenced however we decided to convert this data into presence/absence data due to size of fecal material used.
I've had trouble wrapping my head around the best ways to analyze this data as abundance has been eliminated.
I have been mostly using R and Qiime to work with this data in either the form of biom or an excel spreadsheet.
Thanks for any help you can provide.
Dear colleagues, I am a graduate student in the field of veterinary parasitology. I have a new and innovative idea to treat or prevent parasites that cause cancer in their hosts. But I need help because of lack of funding and testing. I'd be happy if someone would be willing to cooperate, please inform me.
These were all retrieved through a formalin-ether concentration technique. The feces were collected from stray cats (Felis catus) through enema.
The first three eggs are around 35-40 µm while the fourth one is around 60. The fifth one is around 40-45 µm as well
Our guesses are (from top to bottom):
1 and 2 - Toxascaris leonina (but they're too small)
3 - I think they have polar plugs but are reeaaally small
4 - Diphyllobothrium latum
5. Toxocara cati (but again, it's quite small)
Some insight about it could be really helpful. Thanks in advance :)
Please any one can provide me a sequence of specific primer of Theileria camelensis which infect camels or any related method for molecular identification of this protozoan parasite.
Our Group (Laura Jaramillo and me) have two other parasite eggs found in faeces of the river Otter, that are different of the previously discussed
I want to know if STH and schistosome eggs are resistant to freezing and can be detected a second time if forzen feces are reanalyzed by the Ritchie concentration method and Kato-Katz.
I often find these items (marked with red arrows) while doing equine FECs. I am interested to know what they are. I am pretty certain that they aren't parasite eggs, and I find them in samples both with and without strongyle infections. Sometimes the two black parts are offset, giving the appearance of a Micky Mouse head.
To give a scale, there is also a strongyle egg (marked with a dot) in the image which is approx 90 um long.
What is the best statistical analysis for the reinfection of schistosomiasis, given that the data consists of the EPG (eggs per gram) of stool samples of 3 sets of different host species?
The study uses the gray geese to give basic information on the small intestine and large intestine can help to complete measurements in the other experimental study.
I have several articles but all not with a clear description of this item.
In case of mixed infections, how do I isolate various Eimeria species which I plan to use for separate experimental infections?
And do I have to culture the parasites to produce enough for the experimental work? If yes, is there any simple method for culturing of the coccidian?
I am doing a research on ticks (Rhipicephalus microplus). I want to set up an apparatus where I can feed the experimental ticks with fresh blood. Any help will be appreciated.
Diagnosing Giardia could be challenging using conventional parasitological techniques. I looked for commercial kits for Giardia diganose, but all of them are indicated for G. lamblia. Does anyone ever tested them with wild animals?
these two specimen were found parasitic on the abdomen of charybdis longicollis, I need to know the genus and species
the two pictures are ventral and dorsal view
As the blood is going to be imbibed by ticks in an in vitro feeding system, I'm looking for non-toxic methodology (cryoprotectants).
I have looked up quite a number of research articles on this topic; none provide information on how the sample was determined. All these articles choose sample sizes that vary extremely from the other. This leaves me with no space for speculation. Can i get any help from an expert in this field of veterinary parasitology?
I found these structures in a dog's fecal sample examined with sedimentation . 20X weight took this photos, first one's measure is approx 84*49 µm, second one is 68*53 µm. I suspect from Alaria spp egg?
I have a data with 18 sites and 36 species overall. Data set comprises of presence or absence values in binary. And the sample size differs for each site. basically it looks like as the following,
sites sp1 sp2 sp3 .....sp36
site1 (n=28) 5 3 5 .....
site 2 (n=68) 2 0 8
and so on.
Please someone help me,
1. how can i arrange the data for easy analysis in R.
2. which package can be used to measure cumulative species richness
3. to avoid bias would like to do extrapolation to get an exact number of samples through rarefaction between the sites keeping area of the site as a parameter.
The companies address and the email of professors would be good.
I am getting Cryprosporidium parvum oocysts in rat faecal samples after experimental infection in immunosuppressed rats. I need to demonstrate the protozoan in intestinal sections as well. Since this is a superficial parasite, please suggest how should I collect intestines after necropsy for routine histopathological sectioning so as to maximize chances of demonstrating the parasite in intestinal sections.
Does anyone know primers that can be used to amplify all Babesia species in canine blood DNA samples? I have tried RLB primers and all 18S primers with no luck so far.
It was observed that a fly used to lay eggs on the skin of Hog deer (Hyelaphus porcinus) in Kaziranga National Park, Assam, India during winter season as told by wildlife rescue operators. These larvae emerge as adult flies from the host body after few weeks. Can anyone help me in identifying the fly responsible for this infestation? Can these larvae belong to bot flies? Please find attached few images as sent to me by the rescue operators.
I made every changes that I know in the RT-PCR conditions but I still cannot obtain any thing!
Primers are absolutely ok. I examined gradient annealing temperature from 5 C below to 2 C over the Tm of the primers, different concentrations of the MgCl2, Taq, primers and cDNA but ... nothing!
I also changed the PCR and cDNA synthesis kit but ... nothing!
Should I do something before extraction of RNA from Toxoplasma? I use RNX (like Trizol) reagent in similar manner that I do for human cells.
This egg captured from dog feces Its about 100×50 um and ×40 magnified If it possible tell me your detection. Also any ebooks or atlas that help me to detect of dog intestinal parasites, please introduce to me thank you
I would like to know if there is any morphological or life cycle characteristic that allows the differentiation from any avian- Plasmodium spp. and Fallisia neotropicalis in a bird blood smear. I know F. neotropicalis only very rarely is found in reticulocytes.
Is there also anyone who can confirm if any other Garniidae species have been detected in avian species apart from F. neotropicalis?
Thank you very much!
Some oocysts are found by fecal flotation. But I have no idea what they are, and would like to identify them to genus if not species by PCR and sequencing. What are the targeted genes and what are the primers? Thanks!
Can anyone help identify the following larvae. It was found in dog’s stool collected from grass in a park (Europe, Poland)? The feaces were not fresh (excreted max. 2 days before collection).
Sample was prepared using ParaSys Semi-automatic Station for fecal analysis with no staining.
I have always wondered what was behind the evolutionary forces / benefits that lead to this. I wonder if this is the forum to ask this question? Thanks for your input.
We have been trying to isolate the cysts from cow dung and sewage using Zinc sulfate flotation method. But the method only gives very few cysts and also has many debris in it.
I have a parasite infecting the liver in mice model and i could see the parasite in blood stream under the microscope.As it is in blood stream it is difficult to get the images of the parasite by microscopy. I wanted to know how i can isolate the parasite from the blood without contaminating with other cells. How can i prove that it is a free parasite in blood stream.
Your answers would help me a lot.
Now, I plan to design my experiment about hookworm parasite infection. So, I want to know what i should do in blood test for hookworm infection in golden hamster. I saw in many publications that used a measuring hemoglobin level only. Why do the CBC and HCT blood test is not use?
Thank you for your kind response
we need to calculate the number of lambs of different breds to evaluate the phenotipic resistance to gastrointestinal nematode. But we dont´t have experience, could sombady help us?
Can you share your knowledge about Pomphorhynchus tereticollis Rudolphi, 1809 with me?
Thank you, for taking the trouble..
The picture 3 (sample 54) was obtained by flotation, in contrast picture one and two were obtained by sedimentation.
Hello parasitologists, i'm making a proyect about hemoparasites in bats and rodents in peruvian amazon. I finded these protozoans and i don't know its names. Help me please to identify it, thank you.
I would like to identify wide spectrum of Cestode, Nematode and Trematode individuals living in the gastrointestinal tract of micrommamals from central Europe.
I needed the harness to collect faeces for parasite eggs from particular non penned sheep for research.
In our University, there is no faecal collection harness. I have looked at the internet to find and buy one, but have not found any. So, I both some synthetic straps and made the harness myself. Now I would like to publish the method in order to help someone who has similar problem.
In your opinion, does it worth to publish that as a technique?
In the last 10 years we have seen an increase in published cases of proliferative sparganosis and/or proliferative Mesocestoides tetrathyridiosis reported from several host species in many parts of the world. These are tapeworm metacestodes that normally are not asexually proliferative, but seem to become so under some circumstances. It is possible that this is an emerging infectious disease group, but perhaps more likely increasing awareness, better surveillance and more robust field surveys are resulting in more reporting. We continue to work on this in my lab, and I would like to hear perspectives on this, as well as information from those who have observed unpublished cases.
Conference Paper Histology and ultrastructure of acephalic proliferative meta...
Conference Paper GLOBAL PUBLIC HEALTH IMPLICATIONS OF HUMAN SPARGANOSIS
the wolf scats (from isle royale) were collected since 2000, and frozen but have been thawed by other students in the previous years and stored in fridge 4-5 celsius.My project aims to identify helminth eggs.
i performed volumetric dilution using distilled water, analysed 1ml under compound microscope and found no eggs, so i stored the faecal mixture under fridge conditions for a week. i returned to the mixture, took 1ml out and performed microscopy: found alot of the live organisms under x400 magnification. There were about 10 organisms per ml. is it possible that tiny cysts of ..some protozoan flagellate laid dormant for YEARS and only hatched when i applied distilled water? if so, what parasite could this be? or is this just contamination from other students?
I need a method to extract metacercariae of Fasciola hepatica (liver fluke), for microscopic analysis, from herbage samples. I have heard it can be done with household bleach but there is no published method I can find.
I need to quantify larval stages of Taenia pisiformis found in the viscera but I can not find references about this. Thanks
I am beginning training in equine small strongyle identification. The resident expert in our department prefers to use a Beechwood Creosote solution to clear the worms and make their internal structures visible for subsequent identification. It works extremely well and clears the worms within a few seconds. However, Beechwood Creosote is quite caustic and does raise health concerns with my research supervisor. Other researchers use lactophenol or glycerine to clear the worms. How well do these solutions work?
This larva had clearly defined cells except at the very anterior and posterior ends that ranged from what is considered brick-like cells at the anterior end to elongated triangular cells at the posterior end. The esophagus was approximately the same length, the width the same, the length of tail the same as what is more typical of poteriostomum spp. or even Triodontophorus spp. I have included a picture, but was unable to take one with scale bars before the larva swam away (heat treating the larva to "sedate" it unfortunately degraded it's cells such that it could not be found again).
I need to infect the rats with the cysticercoid of Hymenolepis diminuta.