Science topic

Urolithiasis - Science topic

Urolithiasis are formation of stones in any part of the URINARY TRACT, usually in the KIDNEY; URINARY BLADDER; or the URETER.
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I am inducing Ethylene Glycol to the mice to grow urolithiasis and confirmed it by the urine samples by 28 days interval. Now my aim is to know if any increase in volumw is happening by inducing them desired plant extracts. Then i want to measure the actual stone size formed by EG.
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It sounds like you're conducting an interesting experiment! To measure any increase in volume caused by plant extracts, you might consider using imaging techniques like ultrasound or CT scans. To measure the actual stone size formed by Ethylene Glycol (EG), methods like microscopy or imaging can help assess stone dimensions. Consider collaborating with experts for precise techniques in this field. For more details contacting…
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-It's possible?
-Is there too much risk of overheating and damaging the tissues near the stone to be fragmented?
-What are the safest energy values?
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That laser is readily available in veterinary medicine and has been used in human medicine for lithotripsy occasionally.
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One part of my work includes state of osmoregulatory function in patients with urolithiasis. But I only find one paper "Pre- and postoperative osmoregulatory renal function in urolithiasis" V. N. Tkachuk 1982. Maybe anyone know someting new?
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Determination of types of uro-stone in situ by radiology or any other investigations
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Chemical analysis is the only way to confirm. However, a simple kub x-ray can differentiate radio opeque from radioludcent one. Non contrast ct scan, urin analysis and blood chemistry may give some clue.
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Do you know a software for calculating urinary tract stone volume size on a non-contrast computed tomography. It is hard to calculate the real stone volume when stones have irregular shapes - like staghorn stones. Can you suggest an application for this purpose which can work on windows platform. I would especially appreciate the recommendations if the application is a freeware.
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you can use Yawi3D, a plugin of ImageJ. ImageJ and Yawi3D are open source, freeware, multipatform, based on Java 
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It is a very cheap, easy to do model, without radiation exposure. Will it be effective for skill acquisition even with its simplicity?
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Yes, you are right; however it is very expensive, and it is impossible to have in our department.
we perform the puncture without moving the C-arm (in a fixed position), with the X-ray beam perpendicular to the tract. This model give us the same thing and I have used it in many small workshops and the urologist were confident to start puncture and even, some were quicker to perform their first real puncture.
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My main concern is that crystals are not formed or more amount of crystals is formed in this assay.
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The supersaturation of urine with CaOX, the most common component of kidney stones is an important factor in crystallization, with later factors being nucleation, growth and aggregation. Thus if supersaturation or later steps in crystallization can be prevented, then lithiasis should be avoided. Indeed, several measures are usually taken to reduce supersaturation, e.g. increasing fluid intake and medical therapy. Although treatments have improved considerably, it is generally accepted that better strategies for preventing kidney stones have developed.
REFERENCE:
S V Krishna Reddy ,Ahammad Basha Shaik, Suneel Bokkisam. Effect of Potassium Magnesium Citrate and Vitamin B-6 Prophylaxis for Recurrent and Multiple Calcium Oxalate and Phosphate Urolithiasis.
  Korean journal of urology 06/2014; 55(6):411-6. DOI: 10.4111/kju.2014.55.6.411
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I know that Ethyene glycol induced kidney stoneis there another way to induced this model.
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Dear Mudhir, it is easy to induce kidney stones in rats by adding ethylene glycol to the drinking water or by administering intraperitoneal calcitriol in low dose. I have used both methods in my investigation, using adult Wistar rats, and all the animals developed kidney calculi after two to four weeks. However, many animals also presented kidney failure and some died during treatment. More important, most animals presented a heavy burden of crystals within renal tubules. Therefore, I believe that this method does not reproduce accurately what happens in human kidney disease.
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medical management of urolithiasis in recurrent stone formation requires estimation of promoters and inhibitors of stone formation in 24 hours urinary and blood evaluation. Can any one give us the best method in collection of 24 hours urinary sample.
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24 hour urine should be kept in refrigerator or long preservation and sodium azide is generally used to prevent any bacterial and fungal growth in it
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No nocturia , No DM or HTN, he had P.H of surgically removed stone bladder 4-5 years ago. His urinalysis showed mild UTI . U/S showed Multiple stones in the UB and enlarged prostate 40cc.. his flowmetry was 20ml/ sec with no residual urine ,cystoscopy showed larger left lobe of the prostate than the mildly enlarged right one?,what should be next step...
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I would recommend pressure/flow study to determine if he is obstructed even with high flow rate, specially if he has high residual urine. Ultimately he may need bladder outlet reduction procedure plus cystolithotripsy. My preference would be Prostate Photovaporization and Holmium laser lithotripsy.
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Without considering other risk factors
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Obstet Gynecol Surv 2006, 61(11):733