Science method
Ubiquitous - Science method
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Questions related to Ubiquitous
One of the new technologies that has been thoroughly discussed is ubiquitous learning. How do you see its role in teaching and learning in terms of benefits and disadvantages? What are the models to integrate such innovations in your instruction? How do you see the future trends and beliefs of faculty members in this domain?
Hello,
Apologies if there's an obvious answer to this, I'm a first year PhD student and this task is entirely new to me. I'm including a lot of detail to convey my question clearly.
In D. melanogaster, the QF system is used to regulate the expression of transgenic constructs inserted within the genome of a specific fly line (similar to the UAS/Gal4 system).
In this system, the protein QF acts as a transcriptional activator, where in its active form it binds to a QUAS promoter and recruits transcriptional machinery.
One can easily obtain a fly stock which expresses QF from a stock centre like Bloomington. However, I need to design my own vector which will carry an insert that looks something like this:
Tub > QS - P2A - QF
Tub = Ubiquitous tubulin promoter, QS = Repressor of QF, P2A = "Self=cleaving" peptide, QF = Transcriptional activator
For this, I need to obtain the sequence of QS and QF; I have already obtained the former as its wild-type form is sufficient and easily accessible in NCBI.
However, I need a specific modified version of QF designed by Riabinina et al., 2015.
The modified QF protein, QF2w, is less toxic than the wild-type protein and is more robustly repressed by QS.
Is it possible to use online resources to obtain the sequence of QF2w and include it in my vector design? Or is my only option to contact the authors that designed this modified protein?
Best,
Panos
Most entomopathogens that exist on insects and in nature are always in association with a number of other microbes. I strongly feel other ubiquitous microbes have a faster regeneration time compared to the entomopathogens and as a result they usually crowd the media before the entomopathogens can have a chance to start growing. I therefore need media that are specific for isolating entomopathogens alone.
This question delves into the fundamental nature of turbulence, a ubiquitous phenomenon in fluid dynamics that is characterized by chaotic and unpredictable fluid motion. Exploring the mechanisms behind turbulence and finding ways to better understand and predict its behavior is a challenging and active area of research with broad implications in various fields, including engineering, meteorology, and environmental sciences. This question opens up avenues for investigating turbulence models, turbulence control strategies, and the development of advanced computational techniques to simulate and analyze turbulent flows.
Hi everyone,
I am looking if it does exist stantardzed names to call the different administrative level of a country.
So I can classify for different countries relative information according to the administrative level.
According to my knowledge, I have the following sequence : National > regional > province > district >subdistrict. I could not find the reference/origin. I implicitly thought it was ubiquitous, but still, I could not find clear definition of each level to share with some one other.
Do you have any reference for those level, or any other reference name and document I could use ?
Thanks,
Lisa
preferential flow may be ubiquitous, it is difficult to measure and quantify,since the Loess has deep soil layer. Tracers is sultable but expensive for detect the deep soil water content, is there any methods for simulate the soil water content in the deep soil layer?
Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated from a soil sample, we assume the soil is acidic. Is this also applicable to the type of mineral microbes love? How would this microbes tell the presence of such mineral?
Your contribution would mean so much for me
Thank you.
Information is ubiquitous given diverse portals of access online but not always free; some of these resources are made available via library subscriptions. In an attempt to prove their relevance, some academic libraries obfuscate their webpages with extraneous information. They consider this to be a marketing strategy, although the effects are questionable, given complaints of decreasing usage figures. I am interested in understanding what other researchers consider should constitute an effective academic library. Thank you.
It is well known that the golden ratio and Fibonacci numbers are ubiquitous in our universe, and Earth as a physical object is the main subject in geology as a science. So Where can we find the golden ratio on earth and what can we achieve from finding it?
I'm trying to find an easily attainable cell line that is IL-1 responsive and can proliferate in culture. Thanks in advance!
Hello Researchers,
I have problems validating the specificity of an antibody against a molecule. This molecule is endogenus and ubiquitously present in all body and i haven't got a negative control.
While validating antibodies we try different protocols : DOTblot, antibody neutralization but it doesn't work.
Maybe because this compound is coloured (brown).
Thanks in advance
Serena
I am a researcher in the field of fuzzy ontologies in ubiquitous learning.
I ask your help to send me a version of the software (protégé with fuzzy owl2 plugin, gurobi) compatible with Windows 7 version 32 bits. Thank you in advance for your collaboration
Hi everyone, I've just started working on a Project related to the enhancement in the techniques for Ubiquitous Connectivity using LoRa backscattering techniques as my Ph.D. research. My supervisor wants me to work on the LoRa SX1276 chip. So, I am looking to have some useful suggestions here on this forum on how to start my Ph.D. research work that will help me in extracting the research problem soon from the research paper that my supervisor has provided me. I hope to hear from someone helpful soon. Thank you.
Music is ubiquitous, as Anahid Kassabian (2013) has noted in her book "Ubiquitous Listening". People listens to music and different kinds of sounds as their sonic companion for many different activities, like reading, training, while doing sports, working on creative projects, sleeping, relaxing, walking etc. But how is this individual/personal and instrumental/practical use of music changing the way people perceive and listens to music today?
How do you use music and sounds as a companion for other activities? What kind of music/sound and what kind of activites?
The use of the phrase "physiologic pH" is ubiquitous. Yet, if their is heterogeneity of the microenvironment, the phrase is grossly-misleading, by suggesting that "healthy pH" is invariably 7.4. Whereas, we know that the speciation of biomolecules is pH-dependent and solvent dependent.
Things scale in size. Things scale by a scale factor. Relationships scale in particular ways: volume scales differently than area, which scales differently than length. Scaling sometimes seems to occur with a fractional exponent. Scaling is observed in biology, in physical systems, in economics, in networks, and in cosmology. Does the common appearance of scaling in so many different phenomena indicate that scaling is deeply connected to entropy or some other fundamental aspect of the distribution of energy? Why is scaling ubiquitous, and what does its ubiquity tell us, if anything, about underlying principles, if there are any?
I'm having an issue with non specific binding of my primers. I've tried many primers and this is the least messy primer pair but I am still getting a second melt curve peak when doing serial dilutions on the qPCR. The peak is present in a 1:4 dilution at a much lower height and gone at a 1:16 dilution. Additionally, the peak at 82.29 C is higher than the correct peak at 77 C which contradicts my gel (lane 4 after the ladder). This peak is also present at the same concentration in another strain.
Because I am working with bacillus subtilis this cannot be an intron issue and it also cannot be a genomic DNA contamination issue since I have performed (-) reverse transcriptase controls (lanes 7-12 after the ladder) and while there is gDNA, the primers are specific in those controls. Everything has been done in parallel so any contamination should be present ubiquitously.
So I have two questions,
1) what might be responsible other than gDNA for off-target binding in a prokaryotic organism?
2) why would the qPCR machine indicate such a high melt curve peak for a product that according to my gel, is barely there?
The gel isn't run for too long, the correct amplicon is 127bp which corresponds to what I'm seeing in all my lanes and the 83 C melt curve peak corresponds to that larger product I'm seeing in my gel.
The Electronic, Mobile and Ubiquitous Commerce is well established worldwide. With the Digital Currencies and Block Chain, the opportunities to grow global business are boundless. Given the nature of Internet and Block Chain the provision of Client(s) Security and Privacy is critical.
We are studying the expression of some matricellular proteins, mostly in retina. We have the floxed mouse models for those genes and we need a ubiquitously expressed Cre mouse model in which we can expect to find the Cre activity in most, if not all, of the tissues. Also, the Cre expression should be constitutive. Your suggestions are most welcome.
-Golam
Dear research peers,
While dairy NRC (2001) mentions that iron deficiency is uncommon in cows due to ubiquitous distribution of iron, some of the veterinary products marketed in India claims/demonstrates to improve haemoglobin level upon oral supplementation.
Kindly share your knowledge in clarifying the same.
Smart Computational devices and Ubiquitous Connectivity require high bandwidth, which drives Telecommunications Industry to innovate.
The vision for future Humanoid Smart Nano Robotics Devices capable of interacting with humans in ubiquitous and pervasive manner is most challenging and somewhat exciting at the same time.
What are the future research directions in this field of studies?
Computer Science & Engineering Curriculum has been continually evolving and adapting to new cutting-edge technologies and Ubiquitous Access to Internet.
Will future Ubiquitous access to Internet rich and sustain 7/4, 100% availability worldwide?
NGS data provides information on variant allele frequency which is to be used for calculating copies of total cf-DNA, variant copies of cf-DNA per ml.
I have used the method described in
Annals of Oncology 27: 862–867, 2016
Detection of ubiquitous and heterogeneous mutations in cell-free DNA from patients with early-stage non-small-cell lung cancer
authors in this paper, based on the assumption that there are 3.3 pg DNA per haploid copy of genome, have calculated the total cf-DNA, variant copies of cf-DNA per ml.
Is there any other method available for calculating copies of ct-DNA?
Surveillance data are ubiquitous in Latin American countries, but most times of poor quality. I am currently using a mixed-effects model, but should I use Poisson or Linear? I know there are a lot of alternatives for analysis of time series data but these two have the strength that they are the ones that are easier to communicate to the decision makers. Thanks in advance for your response.
the causal mutated genes are almost ubiquitously expressed in many tissues
Ideally would like STRONG widespread expression in dendrites ideally in C57 background.
Hi there, anybody avare of eubacterial primers for real-time pcr, targeting ubiquitous housekeeping gene ? I not looking for 16S targeting primers. I need something elese, some protein coding gene. Thanks a lot for suggestions.
Hoping to get some leads on what to use as a ubiquitous internal control for secreted protein WB
Does any one have references on quality of learning services in ubiquitous learning environment and methods used to compute it ?
India has a huge domain of public transports most of which is decentralized and ill-organized.
Proposed Ubiquitous Transport System would provide a single-window approach to transport facilities where it will be easier for people to avail transport services.
How far is its implementation feasible in the huge and diversified domain of India?
Or is it a good idea bringing all the transport facilities under a single portal?
We are engaged in a metabolic project where we want to express our gene in a whole body and inducible fashion (or at least in important metabolic tissues as muscle, WAT, BAT and liver).
Do anyone know a model to express rtTA ubiquitously?
Thanks
I am looking for related literature that has done research about e-learning in corporate training? how they measure actual learning? What challenges they face when they design and deliver e-learning in corporate training program to improve job performance? If some of you could help, I really be appreciated your help. I couldn't find enough research that answers my inquiries? I am interested in e-learning in corporate training, what is their failure and succeed?
I would like to know the latest IT techniques or research questions (business models, Technologies to resolve..) in the domain of crowdsourcing and citizen science
