Science topic

Tuberculosis - Science topic

Any of the infectious diseases of man and other animals caused by species of MYCOBACTERIUM.
Questions related to Tuberculosis
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Dear Sir/ Madam,
Where Can I get antibody pairs for MPT64 Tests against TB ?
Best,
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MPT64 is one of the major culture filtrate protein (24 kDa) [6],[7] encoded by the RD2 region genes [8] and has been shown to be a specific antigen that differentiates the M. tuberculosis complex from the mycobacteria other than tuberculosis (MOTT) species. [5],[9] An MPT64-based, simple and rapid immunochromatographic assay was developed by the Standard Diagnostics, Inc. (SD) (Yongin, Korea), known as the SD Bioline TB Ag MPT64 RAPID ® test (SD bioline kit). This lateral flow test has been reported to identify the M. tuberculosis complex from the MOTT using the mouse monoclonal anti-MPT64 antibody.
Utility of MPT64 antigen detection for rapid confirmation of mycobacterium tuberculosis complex Arora J, Kumar G, Verma AK, Bhalla M, Sarin R, Myneedu VP - J Global Infect Dis (jgid.org)
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A clinical dilemma in day to day practice in India...
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I don't think so, because this test only evaluate degree of inflammation, not the cause of the disease
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I have a group of women with active TB and wanted to identify if the pregnant ones are at higher or lower risk to develop active TB. Most of the women (84 %) were not pregnant or not in postpartum during TB onset, whereas 6.6% of them were in the postpartum 0-6 months at TB onset, followed by pregnant ones who were 5 % at TB onset. and 4.4 % of them were in postpartum 7-12 months at TB onset. I appreciate any ideas regarding this
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you can review the personal ( file) of the women .
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I will be grateful if someone help me with the anti-tuberculosis assay particularly Luciferease Reporter Phage assay for my work.
I am available to discuss further.
Thank you in advance.
#tuberculosis research
#anti-tuberculosis
#anti-microbial screening
#TB
#TB research
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The National Institute for Research in Tuberculosis- Indian Council of Medical Research (ICMR), is an internationally recognized institution for Tuberculosis (TB) research, India. It is a Reference Laboratory and a WHO Collaborating Centre for TB doing anti-tuberculosis assay particularly Luciferase Reporter Phage assay
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Hi
I am working on a project where I have specific segments of interest. Almost 18 region of TB positive samples were selected from where the mutation can be occurred. For that mutation the specific drug could be resistant. So i wanted to sequenced that specific region applying mini seq illumina sequencing. So i extracted the DNA from left over through Qiagen extraction kit. But when I go for quantification through qubit fluorometer i was surprised that the quantity of the sample is very low . I am confused why this happens. Even though when i pcr that product the quantity is also low . But my pcr was good because the positive control result is perfect. So anyone know or have better solution on that ,please kindly help me
Thanks
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Thank you so much .But we are using pcr kit. All the necessary thinks are mixed up together . So its not possible to check the primer concentration. I think changing the cycle can be a good option although we are maintaining the standard protocol .
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I want an idea about tuberculosis study in Nigeria
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COVID-19 in TB patients
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Hi, I am a research student, In order to find a new enzyme targets to treat tuberculosis, could anyone help me to find out new enzyme targets which should be used only in Mycobacterium tuberculosis and why that enzymes are used as a target for anti-TB.
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Hi,
I am hoping to find general ingredients to add to our E.coli growth medium to increase the plasmid production. I am not looking for a magic ingredient or factor that turns a low-copy plasmid into a high-copy one but rather ingredient(s) to LB/TB (before or after autoclaving) that increase plasmid production /cell in general. We are growing enough biomass with our current medium but I am hoping to increase the plasmid production somehow. Does anyone have any solid lead or experience with this?
Thank you and kind regards,
Alex
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Generally while growing e.coli harboring our plasmid of interest, we incubate the culture for 16 hours. After 12 hours of incubation, you can charge the culture with another dose of selection antibiotic, and finally harvest after 4 more hours. This will keep the bacteria under strict selection pressure to produce the plasmid of interest.
This has helped me.
Best,
Subham.
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I am student of University Malaya, Malaysia,completed MPH. Currently furthering with Doctorate in Public Health(DrPH)
As a part of my course, I am required to complete a 4 week professional attachment from 18th July 2022 till 12th August 2022 to gain more knowledge and understanding in my thesis topic.
The topic of my research is the" EFFECTIVENESS OF VIDEO OBSERVED THERAPY IN THE MANAGEMENT OF TUBERCULOSIS" and the objective of my research is to compare the effectiveness of VOT with Traditional DOTS in the management of TB.  As such, I really would like to observe how a VOT is conducted,what are the platforms available, the pro and cons, issue encountered by TB team etc.
I would truly appreciate it if ANYONE could help me provide the name, contact number and any available details of the clinics where VOT is practiced so that I may contact them for my attachment.  Your help and prompt reply is highly appreciated.
Thank you. Warmest Regards,  Dr Kartik Kaliyana Sundaram
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Thanks a lot for sharing the paper Sir. Got in touch with Dr Maunank,the corresponding author and gathered further insights. Was really helpful.
Trying to explore more options as well meanwhile.
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A 27 year-old married woman was admitted due to pain in lower abdomen for 7 days. USG revealed a multilobbed and multiloculated cyst in the left lower quadrant. During laparotomy, the parietal peritoneum was found thick. It was opened. A cystic lesion containing straw colored fluid was found. loculi were broken. Biopsy was taken. She had amenorrhoea for last one year. 
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Currently usual doses of antifungal drugs are ineffective in most of the fungal infections. We prescribe usually double/triple drugs in higher doses for fungal infections to cure it.
In tuberculosis, there are MDR and XDR tuberculosis. When tuberculosis is resistant to at least two of the most powerful first-line anti-TB drugs isoniazid and rifampin, it is multidrug-resistant tuberculosis (MDR-TB). When tuberculosis is resistant to isoniazid and rifampin plus any fluoroquinolone and at least one of three injectable second-line drugs (i.e., amikacin, kanamycin, or capreomycin), it is extensively drug-resistant TB (XDR TB).
Like that of drug resistance tuberculosis, when should we label fungal infections as drug resistant infections?
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Any assistance?
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Pressing the bacteria with the LC50 intermediate generations. Requires level 4 biosecurity
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I know PCR will be the best method when it comes to identifying TB, but we have well over 200 samples and PCR won’t be suitable, because it is expensive and we want to cut the cost.
do you have any recommendations on other alternatives to PCR that might be cheaper I’m thinking acid fast stain but the only problem is it takes way too long.
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Hevar Neaz,
This published method may be helpful for you
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TB is transmitted through the air. The droplet nuclei generated when a sputum positive pulmonary TB patients coughs, mixes in the air and are carried to a susceptible person in the vicinity or by air currents to longer distances. Sputum Negative TB patients may also contribute in transmission of infection to a smaller extent. Now my concern is that, "Can housefly provides an additional epidemiological link to spread TB infection in the community?"
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Have a look at this useful RG link.
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This is for a meta-analysis using generic inverse variance method and random-effects model in RevMan. The exposure is smoking and outcome is mortality due to TB.
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You cannot combine them in Revman5 but can calculate each and "copy and paste (or screenshot)" by clicking the commands (right above of forest fig, OR, RR, or RD). It is very easy.
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As part of my coursework, we were required to choose a research subject for our end-of-semester project. I chose to investigate how artificial intelligence might be used to identify and predict the presence of tuberculosis in x-ray images. I am looking for research ideas and materials. I'd be really thankful if anyone could offer a suggestion on how to proceed.
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is LC/MS MS appropriate for antigen detection?
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Hello,
You can refer to the research article given below. It will be helpful.
Best.
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I am checking for intracellular growth of TB bacilli in macrophages in the presence and absence of certain antibiotics. The macrophages will be lysed and growth and viability detected using Bactiter-Glo assay in a 96 well plate format.
Could anyone tell me the type and brand of 96 well plates with a flat bottom, opaque walls that is suitable?
Does it need to be tissue culture-treated or do I need a high binding surface? Can I use a non-binding surface?
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Thank you, this information is a great help.
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Hello everyone, I am trying to do a research on the prevention or controlling of tuberculosis in the Pacific region. I would like to know your views to help me prepare for my essay on controlling tuberculosis in the pacific. Please feel free to share your ideas.
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In addition, the major socioeconomic determinants of TB needs to be addressed.
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A person suffering from Tuberculosis (TB) dies today. An autopsy tested reveals that this person also had SARS-CoV-2.
Why is the person said to die from COVID-19 and not TB?
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Tuberculosis is one of the debilitating chronic diseases that exhaust the patient and his immune system, I think here even if the person died due to covid, tuberculosis has increased the susceptibility to infection and the development of severe symptoms of the disease because it exhausted the body's immunity a lot, meaning that tuberculosis became a predisposing factor for that... My sincere gratitude to all.
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Why my BrdU staining shows so many false positive?
My protocol was:
The hippocampal slices were rinsed with 0.05 M Trisbuffer (TB) incubated for 5 min at room temperature with 0.3% H2O2, denatured by incubation for 30 min at 37◦C with 2 N HCl, and blocked by incubation for 1 h at room temperature in 10% normal goat serum (Sigma, USA) in TB containing 0.5% Triton-100. They were then incubated overnight at 4◦C with mouse monoclonal antibody against BrdU (1:100 in TB; Cell Signal, USA) and incubated sequentially for 1 h at room temperature with biotinylated horse anti-mouse IgG (H + L) antibodies (1:200) in TB; Vector, USA), 30 min at 37◦C with streptavidin-horseradish peroxidase (1:300 in TB), and 10 min at room temperature with3,3-diaminobenzidine tetrachloride (Sigma), then dehydrated in ethanol and xylene.
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I assume you are working with mouse hippocampal sections, if this is the case then try to use primary antibody raised in any species other than mouse. Anti-mouse secondary will bind with endogenous mouse IgG and gives false positive signals. As John said, DAB development step can be shorten to couple of minutes, 10 minutes are definitely too much.
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Hi researches,
Currently, my research is focusing on discovering new potent drugs to inhibit M. tuberculosis activity to treat globally serious TB disease. Therefore, I'm looking for a CYP121 gene for protein expression. Can someone suggest me few ways where I can get the plasmid CYP121 gene (pHAT2/cyp121) and E. coli K12 BL21 (DE3) cell for my protein expression and purification research work, please?
Thanks in advance.
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Priya Murugan It seems that the plasmid is not commercially available and it is also stored at Addgene. Usually, if you generate a plasmid and publish your work, you should provide other with the plasmid. However, at the moment a lot of people in Europe are on summer vacation, and we are still in the middle of a pandemic. Maybe, you should give Prof. Munro a bit more time and send a nice email again...
As an alternative:
In the original paper
the insert for pHTAB2/Cyp121 was cloned from a cosmid from Stewart Cole from the Pasteur Institute in Paris, France. You might get the cosmid there as well.
If none of this works, Prof. Liu from the University of Texas also generated a plasmid for expression and purification of Cyp121. They did not use mycobacterial DNA as a template, but ordered the sequence.
If even this does not help, you could consider gene synthesis yourself.
Kind regards and good luck
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I would like to extract Mycobacterium tuberculosis RNA from infected host cells. Is there a method that I can use. Aim is to study bacterial gene expression using RT-qPCR.
I will really appreciate your assistance
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Dear Johannes,
You are going to use the general protocol to isolate RNA. RNA isolation
Starting material: 1* 10^6 📷
Method:
A. miRNeasy mini kit (Qiagen), follow the manufacturer’s instructions.
B. General phenol-chloroform isolation method
1- put your samples in safe lock tubes (larvae sample)
2- Aspirate media and add appropriate amount of QIAzol to each well of cells , then collect your simples in safe lock tupes, and go to step 4.
3- Shake the tubes in the Bullet Blender speed 8 for 3 min
4- leave your samples 3 min on room temperature
5- Add 100 ul chloroform to the tubes and centrifuge them 15min- at 4 degree and 10,8 rcf
6- Remove the aqueous phase to new, fresh 1,5 tube
7- Add 100 ul 2- propanol and shake 15 sec (you will see a cloud like structure when RNA is present). To increase yield, samples can be keep at -20 for a couple of hours or overnight. This step is specially important for zebrafish larvae samples.
8- Centrifuge for 5 min at 4 c degree at maximum speed for pellet formation
9- Discard the solution and wash the pellet with 500ul- 750 ul Ethanol 70%
10-Centrifuge for 10 min at 4ᵒc at maximum speed. Then, a clear white pellet is visible
11-Remove the Ethanol till a small fraction is still covered in Ethanol. Then, Place the tube vertically
12- Let the remaining Ethanol airdry till the pellet no longer white but translucent
13- Add 25 uL RNA free water to the tubes and mix it thoroughly using the pipet.
Note: DNA removal is necessary for certain RNA applications that are sensitive to very small amounts of DNA (e.g., TaqMan RT-PCR analysis with a low-abundant target). Please, Follow the instruction in Appendix E: DNase Digestion of RNA before RNA Cleanup in RNeasy MINi kit.
14- Store your sample at -80ᵒc
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Given that MGIT culture (MGIT 960):
- is susceptibility to contamination
- can produce some false negative
- requires additional budget to keep calibrated MGIT instrument
- require stable power
- is not affordable in developing countries,
do you think that MGIT culture is a reliable culture method?
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Although it has some of these issues that you listed, it is still very reliable in TB research.
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I am working on Anatomical X-Ray Studies of the Lung for Pulmonary Tuberculosis Assessment in Switzerland.
When I was Head of Radiology Department in the City of Urmia Hygiene (Healthcare) Centre (Feb 1984 – May 1984, West Azerbaijan Province of Iran), started the relevant studies for Diagnosis of TB
(Tuberculosis). It was during Iran-Iraq war and flooding refugees from Iraq to West Azerbaijan province of Iran. -We used portable radiography device as “Chest X-ray Minography” at that time. We observe the same phenomenon now as flooding refugees to Europe
and need Anatomical X-Ray Studies of the Lung for Pulmonary Tuberculosis Assessment in Switzerland.
�+
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With great pleasure!
Tina Mamaladze ,cytopathologist from Tbilisi Georgia
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We've run out of TB medium, and I was wondering if it was okay to substitute Super Broth to amplify single colonies?
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Yes, you can. TB media and super broth both are rich media with high peptone and yeast extract contents. TB media also contains additional carbon source. Add 20mM of sterile glucose in super broth.
Using only LB media should also work.
All the best.
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what are the invivo screening methods for anti TB drug?
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I need help from the experts... my research will be conducted in health facilities that deals with tuberculosis patients. the study population is among TB patients newly diagnosed in health facilities. I planned to conduct a quasi-experimental study to assess the effectiveness of intervention in improving adherence to anti-tuberculosis medication where the outcome variable is dichotomous (adhered/ non adherence) based on calculation of percentage of medication ingested. intervention will be conducted at 8 weeks, with pre and post test evaluation. How do I calculate the sample size?
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You could compare difference in proportion adhering (%) before and after intervention. Then to detect a desired difference at 5% alpha significance with power 80% (say) use the standard normal approximation method given in any elementary statistics textbook.
an online calculator is given in the attached ref.
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There are observations that the places where TB is predominant , those country have almost 100% coverage of BCG vaccine for their children mean later stage all adult . Those country are comparatively better fighter in COVID-19 except India !!
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Have a look at this useful RG link.
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My gene is codon-optimized and the 75kDa protein is expressed in BL21 and not in Rosetta. Can there be any specific reason behind it?
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Hi Roger,
Yes, I have used the same condition as I wanted to check in a given condition whether using rosetta enhances my expression compared to BL21(DE3). As my gene was already codon-optimized, I assumed that rosetta would at least express the protein.
Thank you for the suggestion; I will give it another try.
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Hi, I'm quite confused on the type of study design of this research paper (study is available in the attachment)
It seems to be a secondary type of research (does not collect primary data, data source was obtained from previously collected data over a period of 5 years (2015-2020)), the study analyses incidence of TB notifications pre- and post- pandemic from that data source. Would this study be classified as a cross-sectional study or a retrospective cohort study? Any advice would be of much help, thank you very much
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Robert Boer, Mohanad Kamaleldin Mahmoud Ibrahim Babak Jamshidi Thank you everyone for answering. Robert Boer I'm currently conducting a systematic review on the availability of TB services pre- and post- pandemic. I've included that report in my review, and I need to know the study design for reporting on study characteristics and study quality assessment. May I ask how would I assess the study quality for a surveillance study? This is my first time conducting a systematic review and there are a few things I'm still unsure of, any advice would be highly appreciated.
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I have been seeing a patient of RA over the past few months.Patient has few deformities of hands and active disease.She has been managed by non rheumatologists initially.She has received inadequate and intermittent dmards I started her on triple therapy (ssz2gm od,Mtx 20mg weekly and hcqs 300mg of) with a short course of low dose steroids.There was no significant relief so I added iguratimod (25mg od initially and then twice daily). There was no improvement over 3 months so I decided to put her on tofacitinib.Her screening for TB revealed a v strong positive montoux test (25*25 mm). There was a history of being treated for tuberculosis about 20 years back.How to proceed ahead in such a situation
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because it is autoimmune disease?
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I am currently working on colorimetric methods using resazurin and malachite green for diagnosis of TB. I am facing a problem of contamination with aerobic spore bearers after decontamination and addition of PANTA to the samples. Can anybody give suggestions?
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Hi Gulnaz,
Could you control the contamination of your culture? Please share your experience
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Hellow my fellows
Iam working on Real time PCR technique for detection the gene who is responsiable on Tuberculosis as well as the AFB stain so i hope that any one have worked on the same project that can assist me by sending articles or researches that might be useful to complete it .
Thanks
Hammad Shihab
Assistant lecturer
College of education for girls
Mosul University
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Hi Hammad,
What gene(s) have you decided to use to detect TB? Keep in mind that you should run a gold standard (solid or liquid culture) to determine the performance of your qPCR test. Good luck
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I have read a study about risk scoring that can predict the 5 years risk of tuberculosis among household contacts from TB index patients. my question is, can anyone suggest how can i further study by using this risk scoring tool so that i can determine the score threshold for implementing appropriate intervention.
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During follow-up, tuberculosis occurred in contacts of index patients in 120 (13%, ... A simplified risk score including only five variables performed similarly, with ... an in-house MDR/XDR-TB Colour Test thin-layer agar assay, ... tuberculosis within 3 years of the date that the index patient started treatment. Household contacts of patients with tuberculosis (TB) are at great risk of TB infection. ... The incidence of TB disease among the contacts of index cases was 4.4% ... for the individualized prediction of TB transmission among household contacts. ... 5.8 million men, 3.2 million women, and 1 million children (≤15 years) .
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I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow.
I have also tried using 25 degree incubation of overnight cultures and still have the same problem.
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Dear Fiazall
it is quite strange:
1 liter in which flask?
It do not growth or it stop to growth at certain OD?
best regards
Manuele
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With pulmonary TB so prevalent in our part of the world we seen a lot of cases of acute abdomen, or abdominal ascitis or strictures where our suspicion is high for abdominal TB but diagnosing it becomes a challenege. What are the protocols being followed at your institute to diagnose abdominal TB?
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..GI Endoscopy
..Barium studies
..Peritoneal fluid microscopy, Gene Xpert and TB culture
..Peritoneal gross examination and biopsy
..abdominal lymph node biopsy
..look for chest/lungs or other organs involvement
..history of TB contact(may need good inquiry)
..symptoms of fever especially afternoon or evening/night, anorexia, +/-constipation/or diarrhea, weight loss, lethargy,...
These points may help upto great extent in making diagnosis...
If physician is convinced to start anti tuberculous treatment, good response may be noted in 15-45days in majority of patients...important exclusion include drug resistant tuberculosis and other illnesses closely related to TB.
Can consult nearby physician experienced in the diagnosis and management of TB.
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We are planning to diagnose Extra-pulmonary tuberculosis in our lab and we are unable to find the right RT-PCR kit to differentiate typical and atypical Mycobacterium Tuberculosis. If there are any company please suggest us.
Thank you
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@eke692000 u try TB/NTM PCR kits equally use d conventional biochemical test for comparison dis help u 2 know d specificity of dis kits
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Dear all great researchers,
I intend to measure the level of iron concentration in monocytes before I infect them with mycobacterium tuberculosis. The idea is, I want to assess the role of intracellular iron metabolism in TB pathogenesis.
I will be profoundly grateful if you can share a laboratory method to measure iron in monocytes with me.
Hoping to get your usual help with this.
Ebrima.
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You should use Atomic analyzer.
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I have been trying to express a protein using E. Coli strains (Rosetta and BL21) and am using LB and TB media. But both cultures have a very sharp acidic smell. I have tried for two weeks to express my protein but after centrifuge the cell pellet is very honey-like and no protein is expressed on SDS-PAGE!! I have tried plating and there are very nice single colonies.
Can anyone help me in finding the reason?
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Hi,
According to your description, I think it may be fungi or phage contamination during cell culture. It is recommended to strictly sterilize the experimental materials. Or it could be that the target protein is toxic to cells.
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i want to check potential of plant isolate in Tuberculosis, suggest me methods
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in vivo
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Hi
what are the most country around the world have tuberculosis that many cases can recorded daily ?
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Thanks
Its really interactive map
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Tuberculosis is a deadly infectious disease. In 2018, there were more than 10 million cases of active TB which resulted in 1.5 million deaths ("Global Tuberculosis Report" (PDF). WHO. WHO. 2019. Retrieved 24 March 2020). Tuberculosis control programs are mandatory to save lives. I assume due to the COVID-19 pandemic, tuberculosis control programs are getting less attention. Qiao Liu et al. in the attached article opined that the Covid-19 pandemic may impede global tuberculosis elimination goals. In Jiangsu Province, China, tuberculosis notifications dropped 52% in 2020 compared to 2015-2019. Treatment completion and screening for drug resistance decreased continuously in 2020. Urgent attention must be paid to tuberculosis control efforts during and after the Covid-19 pandemic. In this context, in your opinion what could be the possible impacts of COVID-19 on tuberculosis?
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BCG stands for Bacillus Calmette-Guerin. It is a vaccine that provides protection against tuberculosis; it helps build immunity against tuberculosis. However, it is effective in preventing the form of TB that affects the lungs. Covid-19 also eventually affects lungs. So, will it be any relation in between these two vaccines.
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Combined drug therapy for HIV and TB
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Combined therepay for TB/HIV co-infection is effective, for a newly diagnosed HIV with TB, needs to start ant TB for the first 2 weeks, while assessing, IRIS, then we introduce ART based regimen, however remember to effect of rifampicin to DTG, we need to double the dose of DTG, as far as TLD based regimen is concerned, as this medication lowers the level of rifampisin, therefore, be cautius while administer TB drugs with the ART, know the issue of drug-drug interaction, thank you
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SpiNNaker (Spiking Neural Network Architecture) is a massively parallel, manycore supercomputer architecture designed by the Advanced Processor Technologies Research Group (APT) at the Department of Computer Science, University of Manchester. It is composed of 57,600 ARM9 processors (specifically ARM968), each with 18 cores and 128 MB of mobile DDR SDRAM, totalling 1,036,800 cores and over 7 TB of RAM.The computing platform is based on spiking neural networks, useful in simulating the human brain
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Of course, although the key is to chose relevant inputs of data that is uncorrelated. And the decision of what is relevant at the moment is human based
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Vaccinations and screenings for measles, tuberculosis, and other infectious diseases are down in the Corona pandemic. In recent decades, the world has made dramatic progress in lowering the number of deaths from infectious diseases, including tuberculosis, HIV, malaria, and polio. But as campaigns are paused or cut back and as people miss routine care due to the Corona virus pandemic, these illnesses are getting a rare opportunity to come roaring back. The other infectious diseases are spreading in the shadow of the Corona pandemic.
(Source: June 17, Vox)
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I think it is possible to have several fabricated crises due to global conflicts
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I'm writing a review article in which I explain the different mechanisms by which M. tuberculosis reaches a persistent infection by establishing a cross-signal homeostasis with its host, in which both organisms modulate their actions and reactions towards the other one, and at some part I came up with this sentence " This thought provoking notion makes me think of its similarity to Newton’s third law of motion; a system reaches a steady state whenever the forces acting upon it are equal and opposite one another". How prudent is that?
What do you think about it? :)
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The two critical words in the Third Law are 'equal' and 'opposite'. The essence of 'Opposite' may be perceived well in various biological systems.
As pieces of exemplary shreds of evidence, in cancer therapeutics, tumor exerts strong pro-tumor response against applied treatment and imposes therapeutic resistance, one of the major problems seen in preclinical and clinical studies. The same goes for the multidrug resistance in various medically relevant bacteria.
However, measuring/gauging the essence of 'equal' may be a bit difficult task in biological systems.
AFTERALL, BIOLOGY IS A SCIENCE OF EXCEPTION. The rules of physics/chemistry may be applied directly in some cases while in others, they might seem to be bit 'diluted' or rather entwined with greater intricacy... reason: living systems are not isolated systems, which are often considered for the derivation of the rules/laws of physics and chemistry. It is not about the violation of the rules/laws... rather a manifestation of the same in different perspectives- unexplored/unexplained milieu...
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can we use steroids in cases of bronchiectasis with tuberculosis or that may cause bad prognosis ?
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The short response is: NOT indicated
The long response is: It can be an alternative to other anti-inflammatory treatments in exacerbator patients in the case of non-response to this treatment (for example: macrolides), always at the lowest dose possible.
The exceptions: 1. The presence of concomitant asthma. In this case inhaled steroids are needed. 2. Use of systemic steroids (like COPD) in severe exacerbations (usually required hospitalization).
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In many places pandemic has stopped some regular activities like Tuberculosis detection and management which can cost heavily later on in terms of clinical and economical burden on heath care system. Isn't it important to address this issues properly specially in Tuberculosis prone countries?
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Dear Israt,
you posted a very interesting question. Tuberculosis is an impressive sanitary burden since about 5000 years, but alas the Scientific Community treat such a disease as a secondary importance threat, probably because, nowadays, it is mainly diffused in Third World Countries. With this in mind, we can clearly see how Covid-19 pandemic nearly erased other diseases from the current emergencies agenda. In my opinion, you are absolutely right; tuberculosis is a very dangerous pathology, and it could cause a true disaster if we do not remember that Covid-19 is not the only danger. Alas, being the root of such problem a socioeconomic bias, it could be very difficult to bring such dilemma to the international agencies' ears.
Best,
Dave
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Hello
Trying to make Terrific broth. i make total 100ml of TB.  90ml TB and 10ml TB salts. Autoclave separately and cool down below 50 degrees. and add TB salts . But every  time i add TB salts the entire media gets extremely turbid. even if i add 1 ml of TB salts same thing happens....what could be the reason?? 
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Protein mol. WT : 60 kda. Expression in bl21 cells. Induced at O. d 0.8 -1 In TB media at 37 C for 3-4 hours. Yield is lower than untagged protein. This seems to be the case for another protein that has a mol WT of 48 KDA . I checked induction profiles at different temp ( 25, 18 and 30) for the latter but the best yield is at 37 . Both proteins have the expression system ( both are his-sumo tagged). I need to get higher yields for both for crystallization purposes. Ideally, sumo tagging helps to increase the solubility of proteins but I don't know why I get an opposite result. I use 1mM IPTG for the first and 0.2 mM IpTG for the latter ( also optimized). Any help is appreciated.
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Thank you Michael. I induce at around 0.8-1 O. D. . Yes I have done the supernatant - pellet check on SDS page and there is a significant amount in the pellet in both untagged and tagged proteins.. I am actually concerned that the total lysate ( soluble and insoluble protein combined) is less for the tagged as compared to the untagged. And I have seen lower yields with decreasing temp. But I haven't tried cooling down first and then adding IPTG. I can try that. The last option you suggested is what I have been doing, at least with the second protein. I wanted to avoid doing this for both proteins . Takes a lot of labor and is time Intensive , since I am the only one prepping proteins. Not allowed to hire undergrads or masters students now coz that would have been helpful. Thank you nonetheless.
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It is really hard to exactly tell the patients about their neurological recovery patterns. Is there any predictive study, which studied on the mean time of full recovery in patients with CVJ tuberculosis undergoing conservative management.
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Qureshi MA, Afzal W, Khalique AB, Pasha IF, Aebi M. Tuberculosis of the craniovertebral junction. Eur Spine J. 2013;22 Suppl 4(Suppl 4):612-617. doi:10.1007/s00586-012-2497-3
you many find this useful
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The world war started in silence against the unseen enemy since the beginning of 2020. Just created black smokes disturbing global temperature and so many showdowns were completed all over the world to set the accuracy of nuclear weapons for many decades. Nature has showed its revenge by the smallest weapon so far and we are calling it the deadliest one in this 21st century.
We know about the global influenza pandemic of 1918-1919, which killed more than 20 million people worldwide, and the HIV/AIDS pandemic, which began to accelerate in the early 1980s and continues unabated in some parts of the world. In addition, at least 30 other new and reemerging diseases and syndromes have been recognized since the 1970s
New diseases are superimposed on endemic diseases such as diarrheal diseases, malaria, tuberculosis (TB), and measles, which continue to exact a huge toll. Indeed, malaria and TB, among others, are reemerging in a drug-resistant form. Today, infectious diseases remain the leading cause of death worldwide. Many pathogens are becoming increasingly resistant to standard antimicrobial drugs, making treatment difficult and in some cases impossible. Moreover, chronic conditions generally considered noninfectious actually have been found to have a microbial etiology.
New and Reemerging Diseases: The Importance of Biomedical Research
Anthony S. Fauci
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Despite the fact that emerging and re-emerging diseases pose a serious threat to public health in developing as well as developed nations, we are destroying our ecosystem by damaging the forest. We have published many papers on emerging and re-emerging zoonoses. All our papers are easily available at the Research Gate and Academic.
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I'm purifying TB antibody and the columns we are using only seem to have a DBC of about 0.15 mg/ml. This seems very low to me, but I am a protein purification novice. Is this normal?
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I suggest you to look at the protocol down below in the link, would certainly help you:
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I am concerned that there will be a resurgence of preventable diseases such as tuberculosis, measles, rubella and polio due to the Covid 19 lock down. How can this scenario be managed?
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Government of India has launched tuberculosis control program through DOTS. The program is not affected by COVID-19.
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Is there any data on how to manage such patients for their UC?
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Secukinumab may be an option. We have experience in psoriatic arthritis and tuberculosis. Considering the similar IL17 signature of the two diseases, it should be theoretically possible. Don't know of secukinumab data for IBD though.
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Covid infections as of today can be dependent variable. Prevalence of TB or Malaria (per 10000 population) in all countries can be independent variable. The problem is a majority of countries have Covid but zero TB or Malaria. Correlation turns out to be positive at p 0.05 . What could be wrong in my design of study?
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Secondly, whether you include the countries with zero TB, you should both consider the statistical principle and practical meaning of your results. I'm not sure what kind of correlation you used, so suggest you to refer to the statistical book and see if your data met the use condition. If met, then you could consider whether the results of including and excluding zero TB countries are the same. If not, think about the differences and which one is more feasible.
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If one have been vaccinated with BCG vaccine for Mycobacterium tuberculosis, will they test positive for TB skin test even though they are not infected?
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I had the BCG vaccine when I was a kid and have always had a Negative TB skin test.
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Human tuberculosis (TB), a devastating disease caused by the gram-positive, acid-fast eubacterium Mycobacterium tuberculosis, was classified as a global health emergency by the World Health Organization in 1993. TB remains one of the deadliest infectious diseases with an estimated 1.8 million deaths occurring per year, mainly in the developing world (World Health
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Hello dear dr. Yazi .. see attached link please
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It has been reported that Countries with mandatory policies to vaccinate against tuberculosis register fewer coronavirus infections and deaths than countries that don’t have those policies. Another important observation is that; Favipiravir, that is very recently reported to be effective therapy for coronavirus infections, is structurally closely related to Pyrazinamide. Pyrazinamide is the third most important drug (following isoniazid and rifampicin) in the chemotherapy of tuberculosis. What do you think?
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very attractive ideas.
Remarkably, the repositioning drugs that are currently utilized for treatment of COVID-19 either have pyrazine, quinoline s or adenine moieties. Modification of these structures by incorporation of halogens and hydrophobic parts at the vacant positions of the heterocycles will lead to promising candidates as antiviral agents that might be used to fight against COVID-19.
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A researcher wanted to determine the effects of nutritional status on tuberculosis treatment outcomes. There are BMI values at treatment initiation and treatment outcome status on the TB register of previous 2 years.
1 What study design is best?
2 What is the dependent variable?
3 What is the biomarker?
4 Which multivariable model is appropriate for these analyses?
5 What are the outcome measures?
6 What assumptions due we need to check?
7 What is the measure of model fitness?
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BMI is the only thing i have for nutritional assessment. My plan is to compare TB treatment outcome among well nourished and undernourished using BMI. It is secondary data.
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I am from Indonesia and currently, I am interested in conducting a study about stigma for people with COVID 19. In my areas, the eastern part of Indonesia, people are hiding their condition if they have diagnosed positive for COVID 19. People who are known for having this disease will be avoided and even when they die, the community refuses to bury him/her in public Cemetary in their neighborhood.
I am wondering what tools are good to measure the stigma among society. I have tried to search for infectious disease such as TB, is this relevant enough to use it for COVID 19?
Is anyone can give me a suggestion or advice regarding the tools?? Thank you.
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Many studies showed the ability of NMR spectroscopy to distinguish Tuberculosis (TB) Patient.
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Whilst NMR may identify some systemic changes in biofluids as a response to the Covid-19 virus, it is unlikely to progress specific mechanistic understanding. Thus a combination of NMR and MS would be a better approach.
From literature around SARs an MERs, it seems that both cytokine response and lipid transport would be important. There are several metabolic profiling laboratories that are investigating the metabolic response using these technologies including Rutgers and the Australian National Phenome Centre
If multiple centres undertake similar research, there would be substantial merit in creating a broader international database
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A researcher wanted to determine the effects of nutritional status on tuberculosis treatment outcomes. There are BMI values and treatment outcome status on the TB register of previous 2 years.
1 What study design is best?
2 What is the dependent variable?
3 What is the biomarker?
4 Which multivariable model is appropriate for these analyses?
5 What are the outcome measures?
6 What assumptions due we need to check?
7 What is the measure of model fitness?
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Thanks James Leigh !
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Generally, IFN-g levels are very high in tuberculosis patients. And it is expected that pro-inflammatory cytokines will be at higher end while anti-inflammatory will be at the lower end. I have come across a study where they found low levels of IFN-g in TB patients' samples (n<20) compared to healthy individuals. How it is possible? I suggested the patient may be immunocompromised but how can the entire population (almost all samples tested) be immunocompromised? Any suggestions.
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Circulating IFNg levels? I don't know about very high. Usually they are significantly increased but I won't considered them very high. The IFNg at the site of infection is much higher.
You have to post the paper, or post the title and authors. How did they define TB patients, LTBI or active, untreated?
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Th prolong TB treatment has been attributed to the presence of dormant subpopulation. To me, this implies compounds that can reactivate the dormant form could make could adjuvants in TB chemotherapy.
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Dear colleagues,
In my experiment, the part of BALB/c mice infected i/v with Mtb H37Rv at a dose of 106 CFU died 4 weeks later, whereas the other part survived, gradually acquired a healthy appearance and remained behaviorally and externally healthy 2.5 months after infection (until the moment of euthanasia). At the same time, the organs of the surviving animals had high mycobacterial load. Perhaps this is a kind of LTBI model with a high mycobacterial load ? Has anyone encountered a chronic TB model with good appearance of mice and high bacterial burden?
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I also tend to believe that differing death time in these experiments is an issue of technical kind. As to the Mtb burden we registered log 7 in the spleen.
Dear Dr. Shen-An Hwang, your expert opinion was most useful. Thank you a lot indeed for this discussion.
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Is the concept of Tuberculosis Medication really well known to lung experts in India? Do they not mess with the patient's lives by prescribing heavy doses of multidrug therapy? Is it possible to eliminate tuberculosis by 2025 through these medications?
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Its a complex question. Different people would have different opinions. If you think so, start advocacy/teaching experts if someone know better.
I don't think TB could be eliminated in any country. All over world similar kind of regimen are being used. I wish to know why India only is taken into account.
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How can we bridge the gap and increase the tuberculosis detection rate in pediatric age group.
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In the paediatric age group, one needs a high index of suspicion. Here in the tropics, diagnosis is almost entirely based on clinical judgement as laboratory yields are low.
Clinical trials without evidence may breed drug resistance but has been the saving grace for some children in resource poor settings in which we practise.
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Hello good people
I would to infect mice with MtbH37Rv. I do not have an aerosol chamber. I have looked for alternative methods and the method that I would like to employ is via intranasal. I have tried to look for a detailed procedure, however it was with little success. If you have experience on this or have a detailed SOP, may you please assist me. Thank you in advance.
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You have to add 3-6x10^6 bacilli on nostril. We were using 20 microlitre of bacilli from a solution of 3X10^8 cell/ml (1McF) solution. Our published research paper is attached herewith.
Feel free to discuss.
Best
Sam
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Is it thyroid, Pancreas, heart ?Is it absolute? What are the remaining organs not involved?Any data on this available or not? 
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Which part of the lungs TB does not affect
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Deceased professor’s e-mails reveal his ‘loneliness’ in TB research
The news that Koh Won-jung, a professor of the Division of Pulmonary and Critical Care Medicine at Samsung Medical Center, took his own life saddened the medical community in the summer this year. People lamented that “the star of the Korean medical community has fallen.” Koh not only made great achievements but drew much anticipation for his future research outcomes.
Physicians said it was hard to believe that he killed himself because he had more passion for patient care and research than anyone else. Just before he died, he was about to have a fresh start after 18 years of work at Samsung Medical Center.
Koh was exhausted. He worked day and night for 18 years and became an authority in the field of tuberculosis (TB) and nontuberculous mycobacteria (NTM). However, the for-profit system at the hospital did not support him.
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Please let me argue that this case is not an exception, but an event part of a worrying intensifying trend. Increasing privatiozation and deregulation of healthcare markets in Europe and beyond lead to this kind of outcomes:
Rachel Tansey (2017). " The creeping privatisation of healthcare - Problematic EU policies and the corporate lobby push" Corporate Europe Observatory Brussels, 2/6/2017, Available at: https://corporateeurope.org/en/power-lobbies/2017/06/creeping-privatisation-healthcare
One of the side-effects is that private owners might want people to work for less salary but with more workload. A giant actor in the sector recognized the problem: Ana Maria Sedletchi (2019). " 5 things you should know about burnout " - a webinar with Dr. Susana Banerjee at Elsevier Researcher Academy, October 14, 2019 Available at: https://www.elsevier.com/connect/5-things-you-should-know-about-burnout
I would rather quit a workplace than become ill as a result od stress. This case of the professor in question should have never happened! - there might be factors that are not open to the public... Yours sincerely, Bulcsu Szekely
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Hi all,
I tried to express membrane protein MntBC using vector (H-mntB, mntC-H) in E.coli BL21 Gold. Grew cells in TB + 40% Glycerol amp 3L to OD 1.75 under +37C.
Induct with 0.5mM IPTG - 300C 4h.
OD before induction 1.75, OD after induction 1.9
Bacterial mass = 10 gram
SDS-page: 10 mg for Western-Blot, 50 mg – for Coomasie.
I saw a non-stochiometric expression of MntB and MntC using W-B.
Shall I change an expression conditions of the current system or the expression system in general?
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E. coli C41 (DE3) or C43 (DE3) strains seem to work better. The same effect can be obtained in some cases by using lower temperatures for expression or adding very low amounts of chloramphenicol.
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We plan to phenotype acetylation status of some TB patients
How we insure safety of the blood samples
in other word can we use alcohol to sterilize the sample without affecting DNA
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Just take EDTA blood from the patients and extract DNA; just use normal precautions (gloves etc.). Have done this frequently. You may check my papers on TB and protective genes in PubMed, e.g.
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After I did some disc diffusion assays with a fast growing Mycobacteria I now want to do this using M. tuberculosis but I read that you cannot use Mueller Hinton agar with this. Does anyone know why not? Also what is a good agar to use if I want to do the disk diffusion assay with M. tuberculosis that will make a good lawn? Will tryptic soy agar work?
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Ok thank you very much for the information and explanations, Middlebrook 7H11 is the recommended media by E-test, but I was advised that the components in BSA in this media has a potential to bind with my compounds so thank you very much for the suggestions of other media.
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Any information the literature and research work done on pulmonary mycosis and its associated risk factors and susceptibility pattern in tuberculosis patients
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The attached paper deals well with this.