Tuberculosis - Science topic
Any of the infectious diseases of man and other animals caused by species of MYCOBACTERIUM.
Questions related to Tuberculosis
We are using the ROTINA 380 R (Hettich GmbH ) centrifuge machine in our TB Lab. We use it on a routine basis to concentrate TB samples using 2% NaOH. A lab assistant reported to me a few days ago that one of the four bucket is not opening as its cap got stuck due to some unknown reason. He also reported that he already tried different methods like heating up the bucket to 60 C in the water bath, colling at -20 C, and application of WD40 but nothing was working. Please suggest any tips/tricks that we can try to open it. Considering the budgetary constraints and import conditions, it is highly unlikely to get a new one soon. Any suggestions in this regard will be highly appreciated.
I recently tried to induce myocarditis in BALB/c mice using the following protocol:
Day 0: Subcutaneously injected 200 ug of MyHC614-229 peptide (Ac-RSLKLMATLFSTYASADR-OH) emulsified in complete Freund's adjuvant (1 mg/ml m. tuberculosis H37RA).
Day 7: Subcutaneously injected 200 ug of MyHC614-229 peptide emulsified in complete Freund's adjuvant (1 mg/ml m. tuberculosis H37RA).
Day 21: Euthanize and perform histological analysis
While I did not observe the induction of myocarditis, there were signs of inflammation (splenomegaly). One technical difficulty is that I was unable to completely dissolve the myosin peptide in PBS. Many papers indicate that the peptide is dissolved in PBS. Some protocols also use an additional injection of pertussis toxin on day 0.
I am seeking data/documents related to Miliary Tuberculosis (Miliary TB), a rare disease. If you have any relevant resources, please kindly share them with me. Your assistance would be greatly appreciated.
I would like to work on a project on Mycobacterium tuberculosis, but I am worried about the safe conditions for extracting DNA from Mycobacterium tuberculosis culture.
Except LAM test (HIV+TB), no commercial diagnostic kit available. Mtb DNA very low in urine, can metabolic markers or biomolecules be detected in active and latent tb patients which are absent in normal healthy individuals?
Example in trying to determine the proportion (P1 and P2) to use in calculating the treatment outcomes in TB between rural and urban LGAs in South East Nigeria?
Does anyone have a complete protocol to successfully knockout genes in M. tuberculosis and complement back the genes? Please kindly assist.
It is believed that between a quarter and a third of the world's population is latently infected with Mycobacterium tuberculosis. The importance of latency is reflected in a huge drive by research funding organizations to study the biology and epidemiology of latent tuberculosis infection and to create medications that particularly treat latent infection, with the goal of eradicating tuberculosis globally. Mycobacterium tuberculosis' incubation period lasts around 2-3 weeks following the first infection. The objective is to reduce the incubation period so that the patient may be diagnosed and treated as soon as possible.
I will be grateful if someone help me with the anti-tuberculosis assay particularly Luciferease Reporter Phage assay for my work.
I am available to discuss further.
Thank you in advance.
I'm working on preference for tuberculosis preventive therapy. Many thanks.
So, I am in the development of a multiplex qPCR method to detect M. tuberculosis. While it works really well in synthetic DNA as the positive control, the RFU level appears very low in the bacterial isolate DNA as shown in the Figure. Does anybody have experience with this kind of thing and have experience with how to solve the issue?
I have a group of women with active TB and wanted to identify if the pregnant ones are at higher or lower risk to develop active TB. Most of the women (84 %) were not pregnant or not in postpartum during TB onset, whereas 6.6% of them were in the postpartum 0-6 months at TB onset, followed by pregnant ones who were 5 % at TB onset. and 4.4 % of them were in postpartum 7-12 months at TB onset. I appreciate any ideas regarding this
I am working on a project where I have specific segments of interest. Almost 18 region of TB positive samples were selected from where the mutation can be occurred. For that mutation the specific drug could be resistant. So i wanted to sequenced that specific region applying mini seq illumina sequencing. So i extracted the DNA from left over through Qiagen extraction kit. But when I go for quantification through qubit fluorometer i was surprised that the quantity of the sample is very low . I am confused why this happens. Even though when i pcr that product the quantity is also low . But my pcr was good because the positive control result is perfect. So anyone know or have better solution on that ,please kindly help me
Hi, I am a research student, In order to find a new enzyme targets to treat tuberculosis, could anyone help me to find out new enzyme targets which should be used only in Mycobacterium tuberculosis and why that enzymes are used as a target for anti-TB.
I am hoping to find general ingredients to add to our E.coli growth medium to increase the plasmid production. I am not looking for a magic ingredient or factor that turns a low-copy plasmid into a high-copy one but rather ingredient(s) to LB/TB (before or after autoclaving) that increase plasmid production /cell in general. We are growing enough biomass with our current medium but I am hoping to increase the plasmid production somehow. Does anyone have any solid lead or experience with this?
Thank you and kind regards,
I am student of University Malaya, Malaysia,completed MPH. Currently furthering with Doctorate in Public Health(DrPH)
As a part of my course, I am required to complete a 4 week professional attachment from 18th July 2022 till 12th August 2022 to gain more knowledge and understanding in my thesis topic.
The topic of my research is the" EFFECTIVENESS OF VIDEO OBSERVED THERAPY IN THE MANAGEMENT OF TUBERCULOSIS" and the objective of my research is to compare the effectiveness of VOT with Traditional DOTS in the management of TB. As such, I really would like to observe how a VOT is conducted,what are the platforms available, the pro and cons, issue encountered by TB team etc.
I would truly appreciate it if ANYONE could help me provide the name, contact number and any available details of the clinics where VOT is practiced so that I may contact them for my attachment. Your help and prompt reply is highly appreciated.
Thank you. Warmest Regards, Dr Kartik Kaliyana Sundaram
A 27 year-old married woman was admitted due to pain in lower abdomen for 7 days. USG revealed a multilobbed and multiloculated cyst in the left lower quadrant. During laparotomy, the parietal peritoneum was found thick. It was opened. A cystic lesion containing straw colored fluid was found. loculi were broken. Biopsy was taken. She had amenorrhoea for last one year.
Currently usual doses of antifungal drugs are ineffective in most of the fungal infections. Currently we are compelled to prescribe usually double/triple drugs in higher doses for fungal infections to cure it.
In tuberculosis, there are MDR and XDR tuberculosis. When tuberculosis is resistant to at least two of the most powerful first-line anti-TB drugs isoniazid and rifampin, it is multidrug-resistant tuberculosis (MDR-TB). When tuberculosis is resistant to isoniazid and rifampin plus any fluoroquinolone and at least one of three injectable second-line drugs (i.e., amikacin, kanamycin, or capreomycin), it is extensively drug-resistant TB (XDR TB).
Like that of drug resistance tuberculosis, when should we label fungal infections as drug resistant infections?
I know PCR will be the best method when it comes to identifying TB, but we have well over 200 samples and PCR won’t be suitable, because it is expensive and we want to cut the cost.
do you have any recommendations on other alternatives to PCR that might be cheaper I’m thinking acid fast stain but the only problem is it takes way too long.
TB is transmitted through the air. The droplet nuclei generated when a sputum positive pulmonary TB patients coughs, mixes in the air and are carried to a susceptible person in the vicinity or by air currents to longer distances. Sputum Negative TB patients may also contribute in transmission of infection to a smaller extent. Now my concern is that, "Can housefly provides an additional epidemiological link to spread TB infection in the community?"
This is for a meta-analysis using generic inverse variance method and random-effects model in RevMan. The exposure is smoking and outcome is mortality due to TB.
As part of my coursework, we were required to choose a research subject for our end-of-semester project. I chose to investigate how artificial intelligence might be used to identify and predict the presence of tuberculosis in x-ray images. I am looking for research ideas and materials. I'd be really thankful if anyone could offer a suggestion on how to proceed.
I am checking for intracellular growth of TB bacilli in macrophages in the presence and absence of certain antibiotics. The macrophages will be lysed and growth and viability detected using Bactiter-Glo assay in a 96 well plate format.
Could anyone tell me the type and brand of 96 well plates with a flat bottom, opaque walls that is suitable?
Does it need to be tissue culture-treated or do I need a high binding surface? Can I use a non-binding surface?
Hello everyone, I am trying to do a research on the prevention or controlling of tuberculosis in the Pacific region. I would like to know your views to help me prepare for my essay on controlling tuberculosis in the pacific. Please feel free to share your ideas.
Why my BrdU staining shows so many false positive?
My protocol was:
The hippocampal slices were rinsed with 0.05 M Trisbuffer (TB) incubated for 5 min at room temperature with 0.3% H2O2, denatured by incubation for 30 min at 37◦C with 2 N HCl, and blocked by incubation for 1 h at room temperature in 10% normal goat serum (Sigma, USA) in TB containing 0.5% Triton-100. They were then incubated overnight at 4◦C with mouse monoclonal antibody against BrdU (1:100 in TB; Cell Signal, USA) and incubated sequentially for 1 h at room temperature with biotinylated horse anti-mouse IgG (H + L) antibodies (1:200) in TB; Vector, USA), 30 min at 37◦C with streptavidin-horseradish peroxidase (1:300 in TB), and 10 min at room temperature with3,3-diaminobenzidine tetrachloride (Sigma), then dehydrated in ethanol and xylene.
Currently, my research is focusing on discovering new potent drugs to inhibit M. tuberculosis activity to treat globally serious TB disease. Therefore, I'm looking for a CYP121 gene for protein expression. Can someone suggest me few ways where I can get the plasmid CYP121 gene (pHAT2/cyp121) and E. coli K12 BL21 (DE3) cell for my protein expression and purification research work, please?
Thanks in advance.
I would like to extract Mycobacterium tuberculosis RNA from infected host cells. Is there a method that I can use. Aim is to study bacterial gene expression using RT-qPCR.
I will really appreciate your assistance
Given that MGIT culture (MGIT 960):
- is susceptibility to contamination
- can produce some false negative
- requires additional budget to keep calibrated MGIT instrument
- require stable power
- is not affordable in developing countries,
do you think that MGIT culture is a reliable culture method?
I am working on Anatomical X-Ray Studies of the Lung for Pulmonary Tuberculosis Assessment in Switzerland.
When I was Head of Radiology Department in the City of Urmia Hygiene (Healthcare) Centre (Feb 1984 – May 1984, West Azerbaijan Province of Iran), started the relevant studies for Diagnosis of TB
(Tuberculosis). It was during Iran-Iraq war and flooding refugees from Iraq to West Azerbaijan province of Iran. -We used portable radiography device as “Chest X-ray Minography” at that time. We observe the same phenomenon now as flooding refugees to Europe
and need Anatomical X-Ray Studies of the Lung for Pulmonary Tuberculosis Assessment in Switzerland.
I need help from the experts... my research will be conducted in health facilities that deals with tuberculosis patients. the study population is among TB patients newly diagnosed in health facilities. I planned to conduct a quasi-experimental study to assess the effectiveness of intervention in improving adherence to anti-tuberculosis medication where the outcome variable is dichotomous (adhered/ non adherence) based on calculation of percentage of medication ingested. intervention will be conducted at 8 weeks, with pre and post test evaluation. How do I calculate the sample size?
There are observations that the places where TB is predominant , those country have almost 100% coverage of BCG vaccine for their children mean later stage all adult . Those country are comparatively better fighter in COVID-19 except India !!
My gene is codon-optimized and the 75kDa protein is expressed in BL21 and not in Rosetta. Can there be any specific reason behind it?
Hi, I'm quite confused on the type of study design of this research paper (study is available in the attachment)
It seems to be a secondary type of research (does not collect primary data, data source was obtained from previously collected data over a period of 5 years (2015-2020)), the study analyses incidence of TB notifications pre- and post- pandemic from that data source. Would this study be classified as a cross-sectional study or a retrospective cohort study? Any advice would be of much help, thank you very much
I have been seeing a patient of RA over the past few months.Patient has few deformities of hands and active disease.She has been managed by non rheumatologists initially.She has received inadequate and intermittent dmards I started her on triple therapy (ssz2gm od,Mtx 20mg weekly and hcqs 300mg of) with a short course of low dose steroids.There was no significant relief so I added iguratimod (25mg od initially and then twice daily). There was no improvement over 3 months so I decided to put her on tofacitinib.Her screening for TB revealed a v strong positive montoux test (25*25 mm). There was a history of being treated for tuberculosis about 20 years back.How to proceed ahead in such a situation
I am currently working on colorimetric methods using resazurin and malachite green for diagnosis of TB. I am facing a problem of contamination with aerobic spore bearers after decontamination and addition of PANTA to the samples. Can anybody give suggestions?
Hellow my fellows
Iam working on Real time PCR technique for detection the gene who is responsiable on Tuberculosis as well as the AFB stain so i hope that any one have worked on the same project that can assist me by sending articles or researches that might be useful to complete it .
College of education for girls
I have read a study about risk scoring that can predict the 5 years risk of tuberculosis among household contacts from TB index patients. my question is, can anyone suggest how can i further study by using this risk scoring tool so that i can determine the score threshold for implementing appropriate intervention.
I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow.
I have also tried using 25 degree incubation of overnight cultures and still have the same problem.
With pulmonary TB so prevalent in our part of the world we seen a lot of cases of acute abdomen, or abdominal ascitis or strictures where our suspicion is high for abdominal TB but diagnosing it becomes a challenege. What are the protocols being followed at your institute to diagnose abdominal TB?
We are planning to diagnose Extra-pulmonary tuberculosis in our lab and we are unable to find the right RT-PCR kit to differentiate typical and atypical Mycobacterium Tuberculosis. If there are any company please suggest us.
Dear all great researchers,
I intend to measure the level of iron concentration in monocytes before I infect them with mycobacterium tuberculosis. The idea is, I want to assess the role of intracellular iron metabolism in TB pathogenesis.
I will be profoundly grateful if you can share a laboratory method to measure iron in monocytes with me.
Hoping to get your usual help with this.
I have been trying to express a protein using E. Coli strains (Rosetta and BL21) and am using LB and TB media. But both cultures have a very sharp acidic smell. I have tried for two weeks to express my protein but after centrifuge the cell pellet is very honey-like and no protein is expressed on SDS-PAGE!! I have tried plating and there are very nice single colonies.
Can anyone help me in finding the reason?
Tuberculosis is a deadly infectious disease. In 2018, there were more than 10 million cases of active TB which resulted in 1.5 million deaths ("Global Tuberculosis Report" (PDF). WHO. WHO. 2019. Retrieved 24 March 2020). Tuberculosis control programs are mandatory to save lives. I assume due to the COVID-19 pandemic, tuberculosis control programs are getting less attention. Qiao Liu et al. in the attached article opined that the Covid-19 pandemic may impede global tuberculosis elimination goals. In Jiangsu Province, China, tuberculosis notifications dropped 52% in 2020 compared to 2015-2019. Treatment completion and screening for drug resistance decreased continuously in 2020. Urgent attention must be paid to tuberculosis control efforts during and after the Covid-19 pandemic. In this context, in your opinion what could be the possible impacts of COVID-19 on tuberculosis?
BCG stands for Bacillus Calmette-Guerin. It is a vaccine that provides protection against tuberculosis; it helps build immunity against tuberculosis. However, it is effective in preventing the form of TB that affects the lungs. Covid-19 also eventually affects lungs. So, will it be any relation in between these two vaccines.
SpiNNaker (Spiking Neural Network Architecture) is a massively parallel, manycore supercomputer architecture designed by the Advanced Processor Technologies Research Group (APT) at the Department of Computer Science, University of Manchester. It is composed of 57,600 ARM9 processors (specifically ARM968), each with 18 cores and 128 MB of mobile DDR SDRAM, totalling 1,036,800 cores and over 7 TB of RAM.The computing platform is based on spiking neural networks, useful in simulating the human brain
Vaccinations and screenings for measles, tuberculosis, and other infectious diseases are down in the Corona pandemic. In recent decades, the world has made dramatic progress in lowering the number of deaths from infectious diseases, including tuberculosis, HIV, malaria, and polio. But as campaigns are paused or cut back and as people miss routine care due to the Corona virus pandemic, these illnesses are getting a rare opportunity to come roaring back. The other infectious diseases are spreading in the shadow of the Corona pandemic.
(Source: June 17, Vox)
I'm writing a review article in which I explain the different mechanisms by which M. tuberculosis reaches a persistent infection by establishing a cross-signal homeostasis with its host, in which both organisms modulate their actions and reactions towards the other one, and at some part I came up with this sentence " This thought provoking notion makes me think of its similarity to Newton’s third law of motion; a system reaches a steady state whenever the forces acting upon it are equal and opposite one another". How prudent is that?
What do you think about it? :)
In many places pandemic has stopped some regular activities like Tuberculosis detection and management which can cost heavily later on in terms of clinical and economical burden on heath care system. Isn't it important to address this issues properly specially in Tuberculosis prone countries?
Trying to make Terrific broth. i make total 100ml of TB. 90ml TB and 10ml TB salts. Autoclave separately and cool down below 50 degrees. and add TB salts . But every time i add TB salts the entire media gets extremely turbid. even if i add 1 ml of TB salts same thing happens....what could be the reason??
Protein mol. WT : 60 kda. Expression in bl21 cells. Induced at O. d 0.8 -1 In TB media at 37 C for 3-4 hours. Yield is lower than untagged protein. This seems to be the case for another protein that has a mol WT of 48 KDA . I checked induction profiles at different temp ( 25, 18 and 30) for the latter but the best yield is at 37 . Both proteins have the expression system ( both are his-sumo tagged). I need to get higher yields for both for crystallization purposes. Ideally, sumo tagging helps to increase the solubility of proteins but I don't know why I get an opposite result. I use 1mM IPTG for the first and 0.2 mM IpTG for the latter ( also optimized). Any help is appreciated.
It is really hard to exactly tell the patients about their neurological recovery patterns. Is there any predictive study, which studied on the mean time of full recovery in patients with CVJ tuberculosis undergoing conservative management.
The world war started in silence against the unseen enemy since the beginning of 2020. Just created black smokes disturbing global temperature and so many showdowns were completed all over the world to set the accuracy of nuclear weapons for many decades. Nature has showed its revenge by the smallest weapon so far and we are calling it the deadliest one in this 21st century.
We know about the global influenza pandemic of 1918-1919, which killed more than 20 million people worldwide, and the HIV/AIDS pandemic, which began to accelerate in the early 1980s and continues unabated in some parts of the world. In addition, at least 30 other new and reemerging diseases and syndromes have been recognized since the 1970s
New diseases are superimposed on endemic diseases such as diarrheal diseases, malaria, tuberculosis (TB), and measles, which continue to exact a huge toll. Indeed, malaria and TB, among others, are reemerging in a drug-resistant form. Today, infectious diseases remain the leading cause of death worldwide. Many pathogens are becoming increasingly resistant to standard antimicrobial drugs, making treatment difficult and in some cases impossible. Moreover, chronic conditions generally considered noninfectious actually have been found to have a microbial etiology.
New and Reemerging Diseases: The Importance of Biomedical Research
Anthony S. Fauci
I am concerned that there will be a resurgence of preventable diseases such as tuberculosis, measles, rubella and polio due to the Covid 19 lock down. How can this scenario be managed?
Is there any data on how to manage such patients for their UC?
Covid infections as of today can be dependent variable. Prevalence of TB or Malaria (per 10000 population) in all countries can be independent variable. The problem is a majority of countries have Covid but zero TB or Malaria. Correlation turns out to be positive at p 0.05 . What could be wrong in my design of study?
Human tuberculosis (TB), a devastating disease caused by the gram-positive, acid-fast eubacterium Mycobacterium tuberculosis, was classified as a global health emergency by the World Health Organization in 1993. TB remains one of the deadliest infectious diseases with an estimated 1.8 million deaths occurring per year, mainly in the developing world (World Health
It has been reported that Countries with mandatory policies to vaccinate against tuberculosis register fewer coronavirus infections and deaths than countries that don’t have those policies. Another important observation is that; Favipiravir, that is very recently reported to be effective therapy for coronavirus infections, is structurally closely related to Pyrazinamide. Pyrazinamide is the third most important drug (following isoniazid and rifampicin) in the chemotherapy of tuberculosis. What do you think?
A researcher wanted to determine the effects of nutritional status on tuberculosis treatment outcomes. There are BMI values at treatment initiation and treatment outcome status on the TB register of previous 2 years.
1 What study design is best?
2 What is the dependent variable?
3 What is the biomarker?
4 Which multivariable model is appropriate for these analyses?
5 What are the outcome measures?
6 What assumptions due we need to check?
7 What is the measure of model fitness?
I am from Indonesia and currently, I am interested in conducting a study about stigma for people with COVID 19. In my areas, the eastern part of Indonesia, people are hiding their condition if they have diagnosed positive for COVID 19. People who are known for having this disease will be avoided and even when they die, the community refuses to bury him/her in public Cemetary in their neighborhood.
I am wondering what tools are good to measure the stigma among society. I have tried to search for infectious disease such as TB, is this relevant enough to use it for COVID 19?
Is anyone can give me a suggestion or advice regarding the tools?? Thank you.
A researcher wanted to determine the effects of nutritional status on tuberculosis treatment outcomes. There are BMI values and treatment outcome status on the TB register of previous 2 years.
1 What study design is best?
2 What is the dependent variable?
3 What is the biomarker?
4 Which multivariable model is appropriate for these analyses?
5 What are the outcome measures?
6 What assumptions due we need to check?
7 What is the measure of model fitness?
Generally, IFN-g levels are very high in tuberculosis patients. And it is expected that pro-inflammatory cytokines will be at higher end while anti-inflammatory will be at the lower end. I have come across a study where they found low levels of IFN-g in TB patients' samples (n<20) compared to healthy individuals. How it is possible? I suggested the patient may be immunocompromised but how can the entire population (almost all samples tested) be immunocompromised? Any suggestions.
Th prolong TB treatment has been attributed to the presence of dormant subpopulation. To me, this implies compounds that can reactivate the dormant form could make could adjuvants in TB chemotherapy.