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Hablemos de la incapacidad que hoy día presentan miles de adolescentes para ser libres, mostrar sus emociones, su propia identidad, e incluso el sufrimiento, por la falta incomprensión, de educación, de empatía en el entorno o la sociedad, y cómo esto afecta y se presenta en nuestros titulares en España que no conocemos.
  • 7 de cada 10 personas Trans tienen pensamientos suicidas.
  • Las personas LGTB reportan más del doble de autolesiones, intentos e ideaciones de suicidio que la población general.
  • Las agresiones contra el colectivo LGTBI se disparan en Cataluña y baten récords en 2024
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La identidad sexual se desarrolla primero desde la infancia donde el hogar transmite mensajes sobre género a través de roles y comportamientos, los cuales pueden fomentar la aceptación o la limitación de la expresión personal. Y luego en la escuela, en la cual se complementa a este proceso al proporcionar información sobre diversidad y sexualidad, ayudando a reducir la confusión y el estigma. Por lo que un entorno abierto y reflexivo permite que los jóvenes puedan llegar a explorar su identidad de manera como ellos mismos se sientan libres y cómodos, mientras que uno estricto y conservador puede generar inseguridad y afectar a su autoestima.
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Las narrativas transmedia surgen como una opción poderosa que permite contar historias desde múltiples ángulos y plataformas convirtiendo el contenido en algo más que una simple dispersión de imágenes, audio o video, sino en una forma de tejer relatos diversos que, juntos, forman un hilo narrativo rico y complejo.
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Considero que la importancia de las narrativas transmedia radica en su capacidad para expandir una historia a través de múltiples plataformas, permitiendo que cada medio aporte una perspectiva única y enriquecedora. Este enfoque no solo diversifica la forma en que se cuenta la historia, sino que también invita a la audiencia a ser más activa, involucrándose de manera profunda y participativa en el universo narrativo.
En el ámbito cultural y artístico, las narrativas transmedia tienen un valor significativo porque permiten preservar, visibilizar y conectar historias con públicos más amplios y diversos. Al utilizar diferentes medios como videos, redes sociales, documentales, sitios web interactivos, podcasts o incluso experiencias presenciales, se logra captar el interés de audiencias con diferentes preferencias y hábitos de consumo.
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I am synthesizing an organic compound (isoquinolinone) that has both trans and cis isomers, which are very similar in structure. Despite trying various solvent ratios for separation, I have not been successful. What alternative methods or techniques could you suggest for effectively separating these isomers?
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you can deploy Two Minute Separation of the cis- and trans-Isomers of Vitamin K1 without Heptane, Chlorinated Solvents, or Acetonitrile
also some advices from AI Here are a few alternative methods you might consider for separating trans and cis isomers of isoquinolinone:
  1. Chiral Chromatography: This technique uses a chiral stationary phase to separate enantiomers and geometric isomers based on their interactions with the chiral environment12. It's particularly useful for compounds with subtle structural differences.
  2. Supercritical Fluid Chromatography (SFC): SFC uses supercritical CO₂ as the mobile phase, which can offer better resolution for isomers compared to traditional liquid chromatography3. It's also environmentally friendly and efficient.
  3. High-Performance Liquid Chromatography (HPLC): Using a specialized column, such as a C18 or silica column, with an appropriate gradient of solvents might help achieve better separation2.
  4. Gas Chromatography (GC): If the isomers are volatile, GC can be an effective method. You can use a non-polar or slightly polar stationary phase to achieve separation.
  5. Crystallization: Sometimes, crystallizing the mixture under different conditions (temperature, solvent, etc.) can lead to the selective crystallization of one isomer over the other.
  6. Preparative TLC (Thin-Layer Chromatography): This method can be used for small-scale separations. By optimizing the solvent system, you might achieve better separation.
  7. Recrystallization: If the isomers have different solubilities in various solvents, recrystallization can be a useful method to separate them.
Separation of Isomers of Vitamin K1 Using Normal Phase HPLC
Separation of Isomers of Vitamin K1 Using Normal Phase HPLC (thermofisher.com)
Best wishes
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please kindly guide why these extra peaks are appearing. I am attaching mestonova file
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I can't open the file, but is it proton or 31P NMR? Can you post an image of it?
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You can get a FREE ordination certificate and honorary degree here: https://www.dudeism.com/
The ordination actually qualifies you to perform marriages in California (and maybe some other states too). 🤩
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No because a human without a soul is only material(lacking free will, not having the fundamental choice to reason) thus cannot enjoy whatever the soul was exchanged for. To elaborate, without one’s soul, one is cells of the human body and cannot enjoy anything through lacking senses and missing identity.
Sources:
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So. we need the Holy Spirit, which is the substance of man's soul.
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Hi, my lab uses Biorad Trans-blot system and the protocol was set to 25V for 27 mins (for 1.0mm gel). All this while, the transfer works fine with Biorad thick blot filter paper, however recently I changed to Biorad Mini trans blot filter paper.
The transfer does not look as good as using thick paper and i realised that the gel were slightly wrinkled at the bottom after transfer as well. Do i need to use more than 1 filter paper?
Does anyone ever experienced the same situation or does anyone has a recommended protocol that uses Mini Trans blot Filter paper with Biorad Trans- blot system?
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Use two filter sheets on either side of of gel and blot.
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In my wife’s household, which she inherited from her mother-in-law, there is a hen that looks like a rooster, or a rooster that looks like a hen. For a whole year now we have not been able to determine whether it is a hen or a rooster. Probably trans or bisexual. How do you think?
Alexander
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Mother Nature is not always perfect. So, individuals (not breeds) may have imperfections such as both male and female characteristics.
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Hello,
I need to express the PT3TS plasmid, but I have the Trans T1 strain. I tried using Trans T1, but the concentration of plasmid came out to be too low, like 89 ng/ul. So do I need to use the DH5 alpha strain to get more concentration? or the T1 strain is also good for this expression?
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I looked at the genotype of TransT1 and it should be fine. I don't think the problem is the strain but more likely something with the plasmid prep procedure, assuming that the culture is growing well.
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Who agrees genetic engineering should individualize life? Elaborations desired. My answer: I agree genetic engineering should individualize life because queer lives matter and human asexual reproduction supports the LGBTQAA+ community.
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Individualise life? Well by your description it looks to me that sexual relations aren't important.
Nevertheless even if the genetical studies reach pinacle, it will not try to dehumane the love hetros
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Are individual rights COMPLETELY dismissible under the notion they are not absolute? How My answer: No because human rights are linked to diversity, equity and inclusion as described in the following essays:
General Ethics:
General Ethics:
General Ethics:
General Ethics:
General Ethics:
LGBTQAA+ rights:
LGBTQAA+ rights:
Theology:
Theology:
Theology:
Politics:
Politics:
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Ηuman rights that are not absolute are harder to assert. Dismissibiliy is a matter or circumstances, region of application and other factors.
But generally, absolute ones do not get dismissed by claims such as "violation of rights of others by assertng one's rights"
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Hello everyone,
Are the references and bio pages included in the number of pages for the journal "IEEE trans on antennas and propagations". ( I actually have 12 filled pages: 9 pages = 8 + 1 charged page, plus 3 pages which are the references and the bios)
Thank you in advance,
Elie ZARAKET
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Greetings, the maximum page limitation is 15 (in the above insisted journal) and post exceeding which charges are about to incur.
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Hi
Has the crystal structure of all trans lycopene ever been determined?
If yes, the could you please point me to the diffraction data (CIF).
Thanks
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There are some crystal structures of protein complexes with lycopene. Lycopene could be a bit distorted to fit into the protein structure. I don't know if the lycopene is trans, though.
This looks like a light harvesting complex with lycopene.
Download the mmcif file. You can view it in Coot.
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I have a large data set of lead isotopic ratios and I need to determine the unique points where there is (are) distinct changes in those values with respect to time.
How can I do this by applying the above modelling?
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If you are looking for an option in R, Matlab or Python, one possibility is a Bayesian changepoint detection and trend analysis package called Rbeast maintained by me and available from R's CRAN (install.packages("Rbeast")) , Python's PyPI (pip install Rbeast), and Matlab's File Exchange. More info is available at https://github.com/zhaokg/Rbeast. Not sure how useful it ends up being, but you can do a quick testing.
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Does anyone make their own western blot transfer buffer to use with BioRad Turbo Trans blot system? I refer to BioRad catalog number 10026938, 5X TBT buffer to which one adds dI water and ethanol to make 1X.
We would love to minimize costs by making our own, but they don't list ingredients.
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After a bit of trial and error i’m pretty sure the biorad proprietary turbo buffer is just a slightly modified Towbin buffer with some SDS. See attached the 1x recipe I use, and a western I did with the preprogrammed 7 minute turbo transfer (7 minutes, 1.3A, 25V):
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To be used with GC
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I think trans should be from C18:t and C18:2t. You can purchase standards from Merck.
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The above question was posed by great Lucretius (98 - 55 B. C.) and had offered a curious explanation: "This happens because in summer the earth is relaxed by the warmth and any particles it may contain of its own heat are dispersed into the air. ...Conversely, when all the earth is compressed by cold and contracts and virtually congeals, it naturally happens that in contracting it squeezes out any heat it may contain into the wells." [1, 2]
[1] Lucretius, The Nature of the Universe (trans. Ronald Latham), Baltimore: Penguin Books, 1962.
[2] A. Stesenko, "Where is last year's winter?", Quantum (May/June 2001) pp 30-31 https://static.nsta.org/pdfs/QuantumV11N5.pdf
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The water in the well is not exposed to ambient air temperature therefore it does not assume the ambient air's temperature. Ambient air is hot in summer and cold in winter because of exposure to the sun. The temperature of the earth on the other hand is only affected by ambient temperature where there is contact between them. The water also assumes the temperature of any surface in contact with it, which, in this case are the earth on the sides and bottom and ambient air at the top surface. While the top assumes the ambient temperature, the sides and bottom of the waterbody are exposed more to the earth's temperature than the ambient temperature. Therefore they assume the earth's than the air's temperature. Which means the earth's temperature is warmer in winter and cooler in summer than ambient air temperature. What affects the earth's temperature is a question for another day.
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What are the benefits of using the Tran and Blaha modified Becke-Johnson potential (TB-MBJ) to compute the band gap?
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According to information from Wien2k package
"the modified Becke-Johnson exchange potential + LDA-correlation ([Tran and Blaha, 2009]) allows
the calculation of band gaps with an accuracy similar to very expensive GW calculations. It is a semilocal approximation to an atomic “exact-exchange” potential and a screening term".
A good paper to read about the issue is:
First principle study of band gap nature, spontaneous polarization,
hyperfine field and electric field gradient of desirable multiferroic bismuth ferrite (BiFeO3)
Journal of Physics and Chemistry of Solids 148 (2021) 109737
Available
Best regards
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Pin 1 and cyclophilin are two important enzymes in taupathy_relatdd neurodegenerative disorders.
Pin 1 encourages trans configuration of specific x-Pro peptide bonds of its protein substrates.
I would be very thankful, if anwers be provided with references.
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here is the command,
job@job-Latitude-E7440:~/boltztrap-1.2.5/VCu3Te4$ ‘/home/job/boltztrap-1.2.5/src/x_trans’ BoltzTraP
Results
“bash: /home/job/boltztrap-1.2.5/src/x_trans: /bin/csh: bad interpreter: No such file or directory”
Regards.
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Hi. Thank you.
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Re: “Deep Down And Dark - Stawell’s Genius Lair” (Cosmos Magazine, Issue 94) -
On p.34 is the statement, “Because the Earth is physically moving through space as it travels around the Sun, researchers think that we might see a change in the amount of dark matter we see as we move …” This sounds eerily similar to the Michelson-Morley experiment of 1887. This experiment compared the speed of light in perpendicular directions as we travel around the Sun in an attempt to detect the “luminiferous aether”. But this experiment yielded no results to prove a difference between the speed of light in the direction of motion through the presumed aether, and the speed at right angles. Then, less than 20 years later, along came Albert Einstein and Special Relativity with the answer to the puzzle … the flexibility of space-time.
Perhaps WIMPs and so on are the modern equivalent of the aether. The answer to the puzzle of dark matter might be the modern version of space-time’s flexibility … which could be the idea that mathematics doesn’t merely describe the universe, but that the universe is actually composed of maths. Cosmologist Max Tegmark from MIT (the Massachusetts Institute of Technology) is the best known promoter of this idea. My personal belief is that the mathematical universe could be built using binary digits, Mobius strips, figure-8 Klein bottles, Wick rotation, and vector-tensor-scalar geometry.
Another counterpart to aether and WIMPs is the proposed planet Vulcan. In the 1800s (even as early as the 1600s), some people proposed this planet to exist between the Sun and Mercury in order to account for irregularities in Mercury’s orbit (its precession). The need for the planet as an explanation for Mercury's orbital peculiarities was later rendered unnecessary when Einstein's 1915 theory of general relativity showed that these are well-explained by effects arising from the curvature of spacetime caused by the Sun's mass.
Finally, the orbits of certain Trans-Neptunian Objects may likewise have nothing to do with astronomy’s versions of the physical aether and WIMPs (the physicality of Planet 9 or a mini black hole). Mercury’s large precession (due to its closeness to the Sun) may disprove the existence of Planet 9 because a diagram of Mercury's orbit can display an ascending pattern while its perihelion on the opposite side of the Sun maintains a constant level, while a diagram of the orbits of certain Trans Neptunian Objects displays a (less orderly) ascending or descending pattern on one side of the Sun while the greatest part of the orbit of the alleged cause of their perihelia being attracted to more-or-less the same point in space (Planet Nine) is on the other side of the Sun - the actual cause for the attraction of their perihelia would be precession, which affects all objects in the solar system.
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Fig. 1 – Mercury's orbit with its perihelion’s constant level on the left and ascending pattern on the right
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Fig. 2 – Trans Neptunian Objects, a.k.a. TNOs, and Planet 9 with ascending pattern of the TNOs on the left and the supposed cause of the positions of their perihelia - Planet 9, with the dotted red orbit - on the right
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Many thanks, Preston! Your mention of "frame of reference" reminded me of this article I posted on ResearchGate - (12) (PDF) Special Relativity's Consequences For General Relativity, Quantum Mechanics And Quantum Gravity (Can Science And The Paranormal Coexist?) (researchgate.net)
INTRODUCTION / ABSTRACT
Principle points addressed by this article –
1) According to Special Relativity, experiments are overrated by modern science since the truths revealed by experimentation are necessarily restricted to one frame of reference.
2) According to the summary of vector-tensor-scalar (VTS) geometry, interaction of gravitation and electromagnetism produces a momentum in gravitons and photons (and a pressure which is known as mass).
3) It must be remembered that referring to space alone is incomplete. Living in space-time, it’s necessary to add some sentences about the time factor in order to explain the Higgs, the electroweak force, and the nuclear forces.
4) Formation of planets and black holes is directly related to VTS geometry.
5) Dark matter and dark energy are related to quaternion functions in Wick rotation, producing a universe that neither expands nor contracts.
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I'm currently trying to find the differential cross section for the products following proton irradiation of Si. something like the attached plot [Barak et al, Trans. Nucl Sci 2001] but at different proton energies. I believe the reaction is 14 Si(p,X), and I believe the data would be MT=5 in the ENDF data base. But I seem to be stuck in converting ENDF into something that would be easily readable.
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Dear Dr. Dave Hansen,
The following data may help:
ENDF-201 ENDF/B-VI Summary Documentation
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I have two crystal structure of metal complexes one is cis and other is trans. Cis isomer is predominantly formed in room temperature over trans isomer.But when i reflux cis isomer in ethanol its almost fully converted to trans form which is monitored by TLC.Both cis and trans isomers show same uv and nmr spectra as they are different due to position of chlorine atoms. Therefore how can i prove the cis to trans transition by experiment?
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I think , redox properties of both the complex might help you, if your ligand is redox non innocent, then the metal-ligand electron transfer phenomenon may vary between the two isomers.
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I am looking to complement a gene on the genome of K. pneumoniae. I can not complement in trans. Is there a region of the genome that people use to complement genes?
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The "original" attB is the integration site for the phage lambda. But attB is now often used generically to define other phage integration sites as well. There are quite a few different "attB" sites in the E. coli genome, likewise there are quite a few in the Klebsiella genome that are similar to attB but I do not recall whether Klebsiella has the lambda attB specificially or not.
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I will be analyzing condensed tannin extracts for their mDP, PD/PC ratio, and cis/trans ratio using the method of Gea et al. (2011).
For the calculation of mDP, the extension units will be identified as BM adducts (see photo).
Do I need to synthesize standards of these BM adducts? They used NaBH4 in doing so and our lab doesn't have that chemical.
Is there any alternative for NaBH4 or is there another way rather than to synthesize them?
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Lithium aluminium hydride (LiAlH4) is an active alternative to sodium borohydride (NaBH4) in reducing carbonyl compounds. It is more active because the Al-H bond is more polar and places more electron density on the hydrogen than the B-H bond.
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I am confused on whether decalin system can have double bond at bridgehead carbons or not.
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Dear Geetika Arora, decalin has no double bond and no bridge. How are you going to apply such a rule. My Regards
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what instruments are needed to determine and quantify:
  • PD:PC ratio
  • mean degree of polymerization
  • cis/trans stereochemistry?
As much as possible, the simplest instrument is preferred.
This is for condensed tannins from plant extracts.
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Dear Reena Dado, what is your polymer? NMR may be used. My Regards
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I am looking to calculate the difference between the binding affinities of two isomers of a molecule bound to a membrane embedded protein using a method similar to this in GROMACS:
In the tutorial, the transformation is entirely controlled by options in the .mdp file so it's not necessary to specify a B-state in the topology. However in my case where I'm transforming a trans double bond to a cis, I think I would need to include both states in the topology but I'm just not sure how to exactly do it and what .mdp options are needed. Any help/references would be much appreciated.
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Sun Tiedong Okay that makes sense. I was just thinking a small transformation from a cis to a trans isomer would be more efficient. But in this case it's probably better to be more rigorous. Thanks for your answer! It was helpful.
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I would like to know about the tools that could be utilized to explore health care access barrier of trans population in low and middle income settings
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You could use any gender-related tools and tailor/engender them to suit you targeted group, without necessarily reinventing the wheel.
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Lately, many cases of murder or aggression against people in the trans community are emerging. It is already difficult to accept that great change in your body, mind, adapt, so that people also reject you. My own father says that they are "specimens", that one is born with the body "that God has given him", something that I have heard on many occasions from people above all religious.
Also, something that we don't understand at all is how a person can feel like a man and still have a vagina. Really, what is being a man? What is being a woman? They are concepts devised by society, concepts that we have put ourselves, that can be changed, modified. Until the 70s, being gay was included as a mental illness, and it is simply love, for another sex, because it is harder for him to accept trans people. Which I do not understand since, what does the one on the side have to say about the body of another? The main responsible are the politicians, since lately the people who have come to the presidency are "traditional" who defend the family, are closed and uncompromising. Examples like Trump in the US or Bolsonaro, in Brazil.
This is news about a trans boy who was in a coma with only 22 years for transphobic attack
I open a space for reflection and for us to consider what we are doing wrong, which is what fails
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For the religious, I have gone through the scriptures (Holy Bible and Koran) and there is no place where trans are mentioned nor where such gender came from. In scientific analysis, I only see male or female as gender. In the public and/or civil service, I see only male and female. What really defines gender with the trans I do not know and completely refuse to lose sleep over it. I sincerely think they have a lot to answer to the rest of the world.
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I have been wondering about the relative impact/content of a paper I published in 2019 about a dispute within trans studies over Andrea Long Chu. My article, "The Trans Complaint" hasn't circulated much and I wonder the following. What, really, does this material contribute to the wider discussion about trans studies? What does the study of affect and desire provide by way of analysis in the work? Does the role of ordinary language philosophy enable social science to move closer to identification and everyday life? I am welcome to any and all critiques, questions, and the like (minor typographical issues aside).
Thank you,
B
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Thank you. I tend to become an online wallflower when it comes to my work. I will do more circulation!
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Does it affect the reaction if the cis-isomeric form of PdCl2(PPh3)2 is used as catalyst in the Sonogashira Coupling?
Because if we use cis-form then there won't be any requirement of cis/trans isomerization after transmetalation in the Sonogashira !
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Dear Frank T. Edelmann thank you for your answer, and sharing the information regarding the purchase of the chemicals
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I want to perform docking studies on Cis and Trans isomers of a particular molecule (which I have synthesized) in a specific receptor to know how they are interacting. Can anyone suggest the procedure on how to perform the docking studies of Cis and Trans isomers of a molecule or any article which supports this?
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If you are worried about rotation over a bond, make sure that that bond is rigid. It can easily be done while preparing the ligand.
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I apologize in advance if I come across as ignorant. I am trying my best to educate myself on LGBTQI+ community and how to write them in academic writing.
I have a sample of X participants in a qualitative study. One of them identifies as a transwoman. 3 belonged to other sexual or gender minorities. I don't want to end up being insensitive in my writing. Of course, a transgender woman is a woman. What I am having trouble with is that - are the terms "male" and "female" assumed to be cisgendered?
Or,
Should I mentioned that: Y number of participants were cisgendered: M males and N females. And Z participants were from gender minorities (and include trans participants in the second sentence?).
Or should I take the route of M males and N females (where trans participant is included). and Z participants were from gender minorities.
___________________
It took me some time to put forward this question because I feel like I am not able to frame it properly. So feel free to ask if you need clarifications from my side.
Thanks!
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Hello there! May I ask you about your study's theme? If it's something directly related to gender questions, It would be interesting to provide more detail about gender identity (e.g. X were cisgender, Y were trans woman, Z were non-binary, etc etc). But If it's not a Topic directly related to your theme, your second route (Man and woman including trans people) sounds correct as Well. You Also can check other qualitative studies in your field and see How they tackle this question.
Good Luck!
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I have cells seeded on both the apical and the basolateral side of a transwell. I would like to stain both sides to observe the cross talk between both sides. I've been looking for protocol, but have not really found any for staining both sides. If anyone knows of anything that would be very helpful!
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The only meaningful way to do it is to fix the cells, cryopreserve in sucrose, and perform cryosections of your transwell membrane. You will need to coat your slide glass with poly-d-lysine to ensure section adhesion.
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Hi mates,
I wonder on current methods for assessing forest recovery from remote sensing perspectives, anyone can recommend me certain approaches pls? The data will be expected from both optical and LIDAR data. My expected analysis is around 11 years in my study site after a major disturbance. I want to analyze trend in forest recovery rates measured by different indices obtained or generated from remote sensing data.
Kind regards
Bang Tran
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Dear Bang Nguyen Tran,
You can use FVC, minimum NDVI, InSAR techniques to extract degraded forest areas from SAR data. Please follow the link that I have attached below. Hopefully it will fulfill your requirements.
Best wishes with regards
Suraj
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I have not achieved separation of cis and trans fatty acids derivatized as trimethylsilyl esters using N,O -BSTFA on the DB-5 Column, but I prefer this derivatization method as it is rather straightforward. I would like to get a high polarity column for my separation of cis and trans isomers and would like to know if anyone has tried using these columns for analyzing fatty acids derivatized as trimethyl silyl (TMS) esters and if possible, what column is recommended. Thank you
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I was reading "Time-Series Anomaly Detection Service at Microsoft" (https://arxiv.org/pdf/1906.03821.pdf) in these days, and I got some problems for the programming part.
The first picture shows the algorithm, the general idea is to perform the fast fourier transform for a time series sequence, calculate the spectral residual and perform inverse fast fourier transform at the end. When I checked the official code of this paper, before performing the inverse fast fourier transform, the transformed signal ('trans' in the code) was multiplied by the spectral residual and then divided by its amplitude (line 212 - 215 in the second picture) which is confused. If someone can explain about this part? Thanks.
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Multi-access network (especially multi-access edge computing) allows one user to access multiple base stations simultaneously for data uploading [1,2]. Meanwhile, in a cell-free network, there are no cells or cell boundaries, and each user can access all available access points (APs) for data processing [3,4]. So, how they are different from each other?
References:
  1. H. Guo, J. Liu and J. Zhang, "Computation Offloading for Multi-Access Mobile Edge Computing in Ultra-Dense Networks," in IEEE Communications Magazine, vol. 56, no. 8, pp. 14-19, August 2018.
  2. Q. Pham et al., "A Survey of Multi-Access Edge Computing in 5G and Beyond: Fundamentals, Technology Integration, and State-of-the-Art," in IEEE Access, vol. 8, pp. 116974-117017, 2020.
  3. H. Q. Ngo, A. Ashikhmin, H. Yang, E. G. Larsson and T. L. Marzetta, "Cell-Free Massive MIMO Versus Small Cells," in IEEE Transactions on Wireless Communications, vol. 16, no. 3, pp. 1834-1850, March 2017.
  4. H. Q. Ngo, L. Tran, T. Q. Duong, M. Matthaiou and E. G. Larsson, "On the Total Energy Efficiency of Cell-Free Massive MIMO," in IEEE Transactions on Green Communications and Networking, vol. 2, no. 1, pp. 25-39, March 2018.
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In the multi-access scenario described in those articles, the user is accessing the network through multiple access points. Each of them is serving the user separately. In a cell-free network, the access points are jointly serving the user, as if there were only one access point with distributed antennas.
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I just created an absorbance spectra on a hydrocarbon. It was trans,trans,trans-1,6-Diphenylhexatriene. I thought that there would be only one peak, but there were three. Can anyone explain where the other two peaks came from?
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Mohamed Salah Nabet
I read the article but I couldn't find anything.
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If count the water molecule then the point group will be D4H with geometri square planar, but without water molecule it will be d2h with geometri planar-prymidal.
Also for the product I have difficult to understand if the geometri is square planar or not.
What is the coordination geometry of copper(II) ion in cis and trans Cu(gly)2.H2O complexes? What type of d-d electronic transitions would you expect for copper(II) ion with these geometries? Compare with a cobalt(II) ion with similar geometries.
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I'm looking for a researchers who are working or had worked with the transgender adolescent and family therapy processes.
Tranks.
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I need info in companies that can do trans differentiation from blood samples to neurons.
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Dear Liz,
are you talking about transdifferentiation without a pluripotency intermediate? There are very few papers published to this end (see Masuma's answer for one), and the published protocols to date do not seem to be too efficient. Thus, I do not know whether companies (already) enrolled in this. For blood-to-neurons differentiations via the iPSC stage, there should be plenty. Please let me know in case this would still be relevant to you.
Best,
Lea
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This is a simple RC circuit, as shown below:
RC01 .option runlvl=0 reltol=1e-10 vg gate 0 0 vin in 0 pulse -5n 5n 0.0 1us 1us 4.998ms 10ms c1 in out 0.5n r1 gate out 50meg .tran .01us 20ms .print tran v(out) i2(c1) .end
When vg=0V, there is clear and periodic output signal. However, when the vg=3V, the output voltage is constant.
I assume that this is about its accuracy, but how to enhance it to get the ideal output signal?
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Dear Zion,
I could not open the files! would you please display the circuit ans the waveform.
From the principle point of view, the accuracy achieved is a consequence of the accuracy of the circuit models of the used elements and components.
Best wishes
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Dear All,
I need to change the omega angle of some residues in my protein in order to flip them from trans to cis state.
I tried geometry script of VMD but It calculated all Phi, Psi , and omega as zero. and it also doesn't change omega.
I also tried to find servers and plugins to do this. But generally, they correct structure and change cis to trans.
Do you know how I can flip a residue from trans to cis?
Thank you
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This question.
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Considering a Trans boundary as well as Climate disaster prone River Basin, at-least how many years of historical data will be suitable for Future climate scenarios projections?
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In my opinion at least 50 years, especially in arid regions (lack of data)
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Hello All, I am working on human novel lncRNA expression & quantification in different cell types, I build index files ( hg38) with & without --exon --splice-sites by Hisat2, Is their any differences are in the results of CPC2 tool.
Index files build with exon & splice sites
$ extract_splice_sites.py chrX_data/genes/chrX.gtf >chrX.ss $ extract_exons.py chrX_data/genes/chrX.gtf >chrX.exon $ hisat2-build --ss chrX.ss --exon chrX.exon chrX_data/genome/chrX.fa chrX_tran
Index files build without exon & splice sites $ hisat2-build chrX_data/genome/chrX.fa chrX_tran
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Proceed with StringTie, Perform CUffcompare, take transcripts not overlapping coding regions and perform coding potential calculation with third party tools.
Cross check and annotate these sequences with Publicdb.
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I need to calculate the information theory quantities like marginal entropy, joint entropy, conditional entropy and trans information entropy for continuous variables. Where can I find a software package for this calculation?
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Thank you very much
Viswanathan Ramachandran
for your help.
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I have a product with an 80/20 cis/trans cyclohexane derivative and I am looking for a solvent to see if I can isolate one of them. Preliminary with TLC for a later separation using a silica column.
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Back in the old days when I used to pour my own TLC plates, I doped the silica with silver chloride to separate cis/trans isomers. Try 5 or 10 % weight/weight. Good luck!
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I am currently invested in transmedia narratives and in developing methodologies to describe them. Could we consider ARGs as expanded narratives? Or other forms of transmedia storytelling?
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Hi Richárd,
"The field of expanded narrative includes fiction and nonfiction works that present a challenge to a particular form of storytelling, fiction or nonfiction, in terms of the story content, structure, mode of writing or the ways in which the audience engages with the work. How is an approach to storytelling deemed to be ‘challenge’? Works of expanded narrative tend not to be static texts that require sequentially linear acts of reading, however what is important is the artist or commentator’s arguments that articulate the ‘expanded’ character of the work, rather than adherence to a predetermined benchmark. Challenging the form is not the preserve of recent artists or works…” (Whittaker 2017, 51)
“...I make a case for the field of expanded narrative, on the grounds that: (1) it identifies historical, theoretical and tacit relations between practices and approaches not captured by single disciplinary categories, such as ‘art’, or ‘game studies’, or by media-specific labels such as ‘digital’ or ‘interactive’’; (2) While the field of expanded narrative is broad and inclusive, in terms of the spectrum of media represented and intentions and contexts of the practice spanning the commercial to the esoteric, it is defined by ideas and works that present a challenge to its particular form of storytelling, fiction or nonfiction. The challenge has the potential to experientially unsettle the ontological status of what the audience/reader/participant takes to be ‘real’, represented or imagined – expressed by the metaphor of multi-stability…” (Whittaker 2017, 40)
Whittaker, E. 2017. Transitions-Felt: William James, Locative Narrative and the Multi-Stable Field of Expanded Narrative. PhD Thesis
From this perspective ARGs can be defined as expanded narratives because they have the potential to make ambiguous what players consider to be real or imagined. A particular ARG could also be framed as an expanded narrative, if it breaks some of the conventions of the form of ARGs (however you may define this).
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If the conformation of a fluorophore is changed from cis to trans or trans to cis then how its fluorescence lifetime will vary ?
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Thank you so much Prof. Balter for the article.
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Several articles report the use of catecholborane as mediator for stereodirected synthesis. Among products are many boron-free compounds. But, when catecholborane is treated with an alkyne/alkene, usually a terminal alk-e/y-ne, through hydroboration a trans vinylborane is formed... Do you know another synthesis pathways to try the synthesis of new boron-containing compounds?
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Thank you very much! The metal-free borylation is very attractive for me!
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i want to calculate the transition state for a reaction i.e.occur between ethene and trans1,2-butadiene. can anyone guide me that how can i calculate trasition state for this above reaction i.e. trans butadiene and ethene reactant to form cyclohexene
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There are many subtle things to discuss here, but as a brief guideline:
- Generate a good geometry for your TS according to what you know of organic chemistry. If several TS are possible according to regio/stereospecificity, you should try all of them.
- Launch a Gaussian09 optimization job with the keyword opt=(ts,noeigentest,calcfc,loose) using at least a double Z basis set. This is: optimize to a transition state, do not do the eigentest, calculate force constants once to begin the Berny search, and be loose at converging. Generally speaking, I would always use DFT for this step. Your calculation should have one large negative frequency corresponding with the expected reaction coordinate. If not, improve your geometry and try again.
- Further refine your TS by adding extra layers (solvent effects, triple Z basis set, tight optimization) and calculate energies accordingly using the most accurate method you can afford (which should obviously be the same for the reactives, you cant compare pears with apples).
Hope this brief outline helps.
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Question: Interested in knowing how to develop measures for adjusting the land surface temperature, and for mitigating the consequent effects of urban heat islands (UHIs) by managing the land use composition and percentage coverage of the individual land-use type?
Background: In the context of climate change and global warming, it is important to monitor the signatures of urban heat islands (UHIs) and to understand their impacts on ecosystems and human health. UHI arises from the phenomenon of relatively higher temperature in the urban center over its surrounding rural environment.
Advancements: The results of this study suggest that the newly developed model provides an opportunity for urban planners and designers to develop measures for adjusting the LST, and for mitigating the consequent effects of UHIs by managing the land use composition and percentage coverage of the individual land-use type.
Citation: Nguyen Thanh Hoan, Yuei-An Liou*, Anh Kim Nguyen, Ram C. Sharma, Tran Duy Phien, Chia-Ling Liou, and Dao Dinh Cham 2018: Assessing the Effects of Land-Use Types in Surface Urban Heat Islands for Developing Comfortable Living in Hanoi City. Remote Sens. 2018, 10, 1965. (SCI, IF=3.406)( https://www.mdpi.com/2072-4292/10/12/1965 )
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Urban planning with green vegetation
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I have been working on hybrid methods of Invasive weed optimization. Based on these hybrid methods I have published three articles in Soft Computing, IEEE Trans. on System, Man, and Cybernetics: Systems and Applied Intelligence. One is under review in Applied Soft Computing. Please let me know how to approach Professors who are working in Soft Computing.
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You have a good CV. I recomend to see in the job section of researchgate. There, it is frequently offered posodc positions.
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Honestly, there are no studies on sign language poetry in Poland, and thus no research on its relationship with transmediality.
Linguistics of sign language in Poland focuses on the grammatical description of Polish Sign Language (PJM) and its corpus. Therefore, there are no researchers who are involved in the analysis of sign language in its philosophical aspect. Are there any studies on the transmediality of sign language poetry and the philosophical meaning of sign language?
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Thank you very much for your help!
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Does anyone own a full text copy of this citation they would be willing to share? I can not find it anywhere!
Thanks in advance,
Natasha
Laurenson, E.M. (1987). Back to basics on flood frequency analysis. IEAust, Civil Eng. Trans. Vol. CE29, 47-53.
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The flexural behaviour and bending capacity of reinforced concrete members is clearly explained and can be determined with very high accuracy. The solution is found right as reinforced concrete was first used in the middle of the 19th century (already over 150 years).
However, such a solution has not been found for shear capacity of concrete members, especially members without shear reinforcement, though many models have been developed using different approaches.
I believe that the author of each shear model also knows the shortcomings of his model and always tries to overcome them and it takes much time.
Could you tell me what are the reasons for this according to your opinion ? About the approach or methodology, theory, experimental condition, measurement techniques,... If you have experimence in this field, could you also give me some existing problems regarding shear strength of RC members without shear reinforcement.
Many thanks,
Ngoc Linh Tran
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Maybe I can reply to some extent for Chao-Lie. Ning Chao-Lie
Both flexure and shear are mechanical behaviors at the member level (scale), which involves several factors like geometric dimensions, material properties, etc. In general, we use the three fundamental equations to solve the member scale problems, e.g., equilibrium, constitutive model, and compatibility. Obviously, constitutive model bridges the member scale and material scale, and is the most complex part, since the damage, plasticity etc. are all represented in this part. So if we solve the constitutive relationship, the member scale problems can be solved readily.
"The smaller the scale, the more stable it is." means (as far as I understand) for small scale (material scale), we need only to focus on the constitutive relationship, so the problem won't be affected by other parameters like geometric dimensions and external loads.
Back to the original question, why flexure models are accurate but not the case for shear models. In my opinion, flexural behavior mostly relates to the uni-axial properties (purely tension or compression state) of the material point, while shear behavior mostly relates to the bi-axial properties (tension-compression state) of the material point. Evidently, bi-axial properties is more complicated than the uni-axial properties in both the experimental and theoretical viewpoint. It is easy for most of researchers around the world to perform the un-aixal test of concrete but rarely can perform the bi-axial (shear) test of concrete. The experimental equipment and measurement, ect. are difficult to develop. Pioneers in this field, e.g., MCFT by Vecchio et al. from Toronto and STM by Hsu et al from Houston, both started from the experimental investigations of the bi-axial (shear) behavior of a unit concrete panel. Unfortunately, although 40 years past, problems still remain. We didn't even experimentally find out the precise mechanism of shear, how can we propose an accurate analytical model?
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While for sometime now we had been hearing about polyunsaturated as good fats and saturated as bad ones: please correct me for this if i am wrong
now we have varieties within unsaturated fatty acids?
So which one is better and what should be avoided ?
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Hello, Trans fats are well known to cause bad cholesterol, heart disease, and obesity. The very thought triggers images of deep fryers, tubs of margarine, and other trans fat foods, like those mysteriously shelf-stable packaged cakes. It’s so universally accepted that trans fats will damage your health, that the FDA declared a trans fat ban, with June 2018 as its deadline for food manufacturers to remove artificial trans fats from their products. We can talk very long about trans fats.
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Does anyone ever synthesize this complex (as attached in the files below) before? Any recommended procedure? How to control the product formation by getting only the trans complex?
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i am using aprotic solvent like dichloromethane at room temperature . Yet i still cannot get a NMR with only trans complex. By referring to the journal recommended by Jürgen Wintner , the author only have solid state NMR result. Does this means that the complex is not stable in solution state? Or anything i left out to be cautious when synthesize this complex?
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Bashey's 2004 article (Trans Am Fish Soc 133: 1516-1523) states that they used a laser pointer and a dissecting microscope, with an appropriate filter ("dielectric interference band-pass filter at 590 nm with a width of 35 nm"), to detect fish stained with Alizarin Red. Has anybody else used that method and found it effective? Thanks, Paul.
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Alizarine Red can only used for the identifying skeletal part of the fishes. For larval form it can be used for counting spines, bone and cartilage parts.
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Hi,
We are trying to produce antibody for ELISA assay against a soluble protein shed from the surface of the cells. We know that the when the protein shed from the surface it contains the trans membrane region. I have two Question:
1. Should we include the trans membrane region in our antigen design to immunize the mouse ?
2. If I want to use the same antibody for studying the surface protein later, and the antibody include the trans membrane region, would that be a problem?
Thanks.
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I suggest to run a hydrophylic plot of the protein https://web.expasy.org/protscale/
Leader sequences for secretion and membrane spanning domains should be removed, some are toxic when expressed in E.coli. Stretches toxic are at least of 12-15 aa hydrophobic
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Hi everyone,
I am wonder if anyone know codes or the ways to down download Landsat Surface Reflectance dataset (4/5; 7 and 8) automatically in Python? or R? or Matlab?
I need to download datasets for around 10 Landsat scenes in Victoria, Australia from 1983 to 2017 so it would be very useful if anyone can help to guide me the ways for downloading them automatically
I have made alot of attempts with R codes (getSpatialData-master; nasadata-master; getlandsat-master) Python (espa-bulk-downloader-master; theia_download-master; landsat-util-develop); mut they did not work for me
Thank you very much
Bang Tran
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I am Anh Tran, doing research on entrepreneurial intention two...I am interested in your project...Maybe we can collaborate...
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Thank you
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I would like to get some references about the risks of Information and Communication Technology (i.e. cloud computing, portable storage devices, and electronic trans.) on accounting and auditing
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Outside threat such as intruders, or hackers, may target companies for illicit gain or other malicious purposes. Recent security surveys have shown that a large number of information security breaches originate from inside the organization. Employees can inadvertently harm the organization's systems by deleting important files, opening e-mail attachments that contain viruses, or attempting to fix malfunctioning devices without adequate knowledge or training.
Source:
The impact of information technology on internal auditing.
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I have a NGS data for whole genome of an organism. Actaully we did error prone PCR (eP PCR) for the whole genome. The Data we got have all the trans versions and transitions after error prone PCR. Now I want to calculate the number of unique variants possible after the error prone PCR. Pedel AA is our software of choice. But first we do not have the mutated genome sequence( after ep PCR).The data we get have only wild type sequence.
The result file we have contains all the substitutions possible, but we do not have mutated sequence. Second we do not have the mean number of nucleotide substitutions per daughter sequence which is an important input parameter for Pedel AA.
If we had done the capillary sequencing, we would have a mutated genome sequence after ep PCR and also we could have easily calculated the mean mean number of nucleotide substitutions per daughter sequence.
Kindly help me and let me know how we can calculate the mean number of substitutions per daughter sequence from this NGS data, which have given only wild type sequence.
Thanks
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May be a difficult to interact subject.
But must be interesting for many-2
working on such issues!
However what would we get finally?
Kindly give your ideas/ projection on the
theme of discussion for all those new to
the subject.
Hoping to be educated in the matter &
may continue to interact, encourage the
Younger generation .
BWKRgds
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I have multiple hydroxyl which protected by ester and remaining free hydroxyl need to be alkylated. Can anyone suggest us which will be the suitable condition for the O-alkylation?
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Hello joseph,you can use potassium carbonate and Acetone for o- alkylation.
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I do know that there are some frustrated systems that plainly can't be optimized to a genuine 0 K state. I'm familiar with the work done by people working on instantaneous normal modes (INM) and inherent structure (IS). I'm getting imaginary frequency normal modes, which I knew was possible. But shouldn't I still see the 0-frequency translational and rotational modes? I've looked carefully and can't seem to find anything on this.
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I guess what I was really asking was more of a physical question than a question about GROMACS. Because of the physics of a (locally) harmonic central potential, the Hessian components are not all independent. So I should see 6 singular values, i.e. one eigenmode for each independent translation and rotation of the whole system that leaves the potential unchanged.
Do you happen to know if these singular value normal modes can be shifted to negative energy as a result of error in the Hessian? I guess I should mention that these are collective modes of an entire system with about 80,000 degrees of freedom, about 27,000 atoms. Not modes of a subset of atoms.
Thanks!
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I'm now doing research on Transmedia and I'd like to know existing "quanta" to analyze communication intensity, knowledge building efficiency (Tweets?, Forums? Memes? Nemes?)... Any suggestions?
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I am working in a paper about transmedia and expanded content consumption habits in TV series and film. Has anyone done any research on the field?
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Research Project Zurich October 2013 - Looking for partners and advice
CAST/ Audiovisual Media at the Zurich University of the Arts currently develops a research project in the field of transmedia storytelling. We would like to analyze the levels and forms of audience participation around the Swiss transmedia and ARG project "Die Polder" (October 2013, Zurich) we observe and monitor the project and look how transmedia stories can activate and engage the audience. We are looking for partners to cooperate. You have experience in transmedia research and/or have knowledge in new transmedia audience measurement methods. We would be also thankful for references and examples of transmedia evaluation and research.
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I was hoping to get in on this research a few years ago, regarding Kinesthetic Empathy I am just fascinated. http://www.watchingdance.org/
There are so many ways to measure engagement.. basic heart and breath rates are always impressive. Neuroscience can look at what parts of brain are lighting up, or transmitters are activated, defining an intellectual, visceral or emotional response to content. There are endless possibilities.