Transformation - Science topic

Dear Researcher,

I hope this message finds you well. As a valued member of the academic and professional community, your insights are incredibly important. I am conducting a study on the transformative role of Generative AI in knowledge work, and your expertise could provide valuable perspectives to shape this emerging field.

Future-Proof: Innovative Approaches to Management and Digital Transformation in Modern Business

The digital era has transformed business operations and management practices, necessitating innovative strategies to stay competitive. This book explores how digital transformation reshapes modern management, leveraging advanced technologies, AI, and sustainable practices to drive business performance and growth.

Interested authors are invited to submit a two-page chapter proposal outlining the chapter's goals, methodology, and expected outcomes by November 30, 2024. Full chapters are due by February 15, 2025. All submissions will undergo a double-blind peer-review process.

We look forward to receiving your contributions to this insightful volume on innovative approaches to management and digital transformation.

Digital transformation is reshaping industries worldwide, and the creative and tourism sectors are no exception. For these industries, the transformation of Human Resources (HR) is a critical component to achieving innovation and sustainable growth in a competitive global market. However, the journey is fraught with challenges, such as resistance to change, skill gaps, technology adaptation issues, and financial constraints. At the same time, this transformation offers significant opportunities, including improved operational efficiency, enhanced employee engagement, and access to global markets. This question aims to spark a discussion on effective strategies and best practices for addressing the barriers to HR digital transformation while capitalizing on its benefits. How can organizations ensure a smooth transition, build digital competencies among employees, and maintain competitiveness? Furthermore, what role do leadership, culture, and technology play in facilitating successful digital transformation in HR? Insights and case studies are welcome.

Business requirements analysis and process modeling aid in digital transformation by outlining changes, identifying pain points, and facilitating stakeholder engagement, leading to smoother adoption and reduced resistance to change.

Hi everybody,

I had originally created a box plot (which I will attach down below) which showed highly skewed data. I read that one of the options to make it easier to interpret was to perform log transformation of the Y axis, which I did on R with scale_y_log10().

On the figure in which I had not performed log transformation yet, for the value of 4 (X-axis) the median was either 0 or very close to 0 and there was no lower quartile; but when I performed the log transformation, it shows that the median is actually somewhere in between 6 and 7.

My question is, if the median was 6/7 all along, shouldn't that have shown in the first graph since it is not a really low value? Does it mean that my interpretation of the box plot changes because I performed log transformation, or do I interpret it the exact same way as I would have interpreted the original one?

Thank you!

Could you recommend some articles on Scientific Research Paradigm Transformation?

The Schwarzschild metric describes the gravitational field of a spherical body. The special theory of relativity, based on Lorentz transformations, has a number of experimental confirmations. Is it possible to apply Lorentz transformations to the Schwarzschild metric? This was done

for the linearized isotropic Schwarzschild metric and the geodesic equations for the resulting metric were found. When using them to analyze the frequency shift data of the Pioneer 10 signal, it is concluded that the annual frequency variations are caused by a change in the velocity of the time flow on the apparatus from the point of view of an observer on Earth.

The resulting metric is used to determine the active gravitational mass of a cloud of rarefied gas of relativistic particles.

As the velocity of particles approaches the light velocity, this mass increases indefinitely compared to their total relativistic mass. This result can be extended to relic neutrinos. Since the minimum rest mass of neutrinos is not found, this leaves open the possibility that they may make up a most of dark energy.

I'm preparing the research about interpretation of automation, digitalization and digital transformation. I'm interested in different points of view in this question

Hi everyone,

I’ve been trying to insert my 400 bp modified gene into the PENTR/D-TOPO vector for the past month, but I’m having difficulty confirming the product through sequencing, which is preventing me from proceeding to the LR reaction. Here’s a summary of my efforts:

I’m attempting another transformation, but the same issues persist.

Does anyone have suggestions or insights on how to resolve this?

Thank you!

Hello I am using two expression vectors (pCAGGS and pCDNA) for transforming a fragment of gene (2kbp). For transformation, I am using NEB 10 beta-competent E.coli cells. Issue? Although my gene of interest is transformed into the expression vector, a portion of the expression vector's nucleotide sequence is being removed or deleted.

Could anyone share their experience on this and how you overcame this problem?

Thanks in advance!

There is now a very popular trend for academic journals to offer the open access (OA) publishing option. So, most journals now are transformative journals, that is they offer the traditional no-fee publication as well as the expensive OA publication option. I wonder what will happen in the future? Will all journals soon switch to the OA model? Will all authors be required to pay a fee such as $1000? Do the fees vary by country? It seems that scientific publication will soon become something that only well-funded authors can afford. So, then, what will be the options for those authors who are not well-funded?

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The change from a white background to a black background in your interpretation of the Heirloom Seal of the Realm could carry various symbolic meanings, especially in a cultural or artistic context.

Purity and Clarity: In Chinese culture, white often symbolizes purity, honesty, and peace. If the original Heirloom Seal had a white background, it could represent the purity of the emperor’s divine right to rule or the legitimacy of the Mandate of Heaven.

Brightness and Visibility: The white background may also symbolize transparency or the clear distinction of rightful rule. In this case, the seal would be easy to see and recognize as a symbol of authority and power.

Mystery and Power: In contrast, the black background could symbolize mystery, the unknown, or even hidden power. It may suggest that the rise of this new emperor comes in times of uncertainty or darkness, where power must be reclaimed or revealed.

Authority and Depth: Black is often associated with strength, sophistication, and authority. It might imply that the new rise to power is more profound and possibly more absolute. The black background could indicate a deeper or more intense form of rule compared to the original.

Transformation or Rebirth: The shift from white to black might also indicate a transformation or rebirth — a transition from an old era of purity or peace to a new era marked by challenges, strength, or even secrecy.

Change in Leadership: If the seal’s background has changed, it could symbolize a shift in the nature of leadership itself. The rise of a new emperor could suggest a more assertive or powerful figure, emerging in a time of complexity or mystery.

Restoration or Return of Order: Black could also symbolize a return of order through strength. In a tumultuous or chaotic time, a figure rising with the black-backgrounded seal might represent the restoration of control or an authoritative response to chaos.

If this version of the Heirloom Seal with a black background is part of a modern or conceptual re-imagining of the original artifact, the artist or creator might be drawing on the symbolism of darkness as a powerful, commanding presence. It could indicate a new phase of power, mystery, or the anticipation of something profound and transformative.

The reimagining of the Heirloom Seal in this way might resonate with current political or cultural narratives about the nature of leadership, authority, and how power must adapt to changing times.

....

Hello everyone,

I’m currently working on a Data Envelopment Analysis (DEA) project and I’m unsure whether to use original values or natural logarithms value for my input and output variables. I would greatly appreciate any insights on this topic.

Specifically, what are the advantages and disadvantages of each approach? Additionally, if anyone could recommend relevant studies or literature that discuss this issue, I would be very grateful.

Thank you in advance for your help!

What is the impact of digital transformation on human resources?

I am seeing expression of some gene into disease group comparing to healthy control group.Mean value of healthy couldn’t come exact 1.00...It is now 1.05...

Is there any method for transformation from 1.05 to 1.00 in relative expression?

The Relativistic Doppler effect has been explained and derived from the invariance of the wave equation in the case of light (or from Lorentz Transformations). In relativity, it was described as a phenomenon involving two different inertial frames, a consequence of Lorentz invariance.

Other simple methods have been used to give account to the Doppler effect for waves in acoustics.

Acoustic waves in material media, on the other hand, are neither Galilean or Lorentz Invariant.

The Longitudinal Doppler effect in Nature is a detection of a frequency shift of oscillations originated by a transfer of a net energy and momentum due to non stationary positions of Emitters and observers.

It is properly obtained by adopting the conservation of energy and momentum of waves and matter interacting.

The Doppler Radar unveils a potential issue if one considers inertial both RADAR and a mirror, unless placing some external pressure, to a mirror of finite mass, which exactly counterbalances, the radiation pressure.

It is very interesting also that, according to a very recent work by Hrvoje Dodig,

the wave equation for stationary observers and sources cannot have the same form as the one for moving sources or observers for example.

Such feature should be related also to the fact that ENERGY AND MOMENTUM variations are involved and they play a role which may not preserve the wave equation form

Other questions are related:

Hi Everyone,

(Warning: A bit of a long post due to the necessary background information, sorry in advance.)

So I am in the process of creating a phage display library for my dissertation research, and I have been having major difficulties in producing a significant number of E. coli colonies after electroporating my final ligated product (a 5kb phagemid backbone with a ~800bp scFv insert) into electrocompetent E. coli cells (strain SS320) that I made and tested with proper controls myself. I have been doing months of optimization, and believe that I am close, but recently realized where a potential issue may be that has been hiding right under my nose. During the initial digestion of the phagemid backbone, for which I use Not1 and Spe1, I found that I also needed to treat with calf intestinal phosphatase (CIP) in order to remove the leftover phosphate group located on the resulting sticky ends. The reason for this being that there was still a significant amount of background (colonies containing undigested, supercoiled phagemid) still present when a portion of the digestion reaction was transformed into the previously mentioned E. coli strain. As a quick side note, I understand that Not1 and Spe1 have different recognition sequences that when cut shouldn't pair with one another and re-ligate, but for whatever reason the addition of CIP helped eliminate this background. Moving on, because of the CIP treatment, I know that there will still be nicks in the DNA backbone even after successful ligation of my insert (one on each end), which was also digested by Not1 and Spe1. Could these nicks be preventing my ligated product from supercoiling and, thus reducing its ability to be transformed into cells. As a another side note, I have looked into and tested a nearly exhaustive amount of other possible variables all along the entire cloning process that could be resulting in these poor transformations, and I believe I am narrowing in on the potential cause and this particular issue is one of them. In short, if anyone could give me any insight as to whether or not this nicked DNA backbone could be the reason for awfully low transformation efficiency I would very much like to know so that way I can look into using a different restriction site within the MCS of the phagemid that wont require CIP treatment. Thank you all so much in advance for any and all help :).

I need to perform yeast cell transformation after Golden Gate assembly in a 96 well plate. The cassette will be excised from the vector by RE digestion before transformation and usually, we clean the DNA by precipitation before transferring it into the cells. However, we are trying to avoid this "in between" step and I'd like to know more about alternative and faster purification/enzyme deactivation methods. Or else, what are the chance of success in case I jump straight to the transformation step?

Any suggestion, tips or comment are very welcome.

Thank you :)

My first equation in the Transformation equations for the fifth dimension says that the increase in the mass of the proton is two times, and the Lorentz equation says 10,000 times. Which one is more accurate according to CERN’s experiment to accelerate the Proton?

I performed a transformation, and there were no issues or contamination in the co-cultivation media. However, when I transferred the plants to SI media (MS medium supplemented with sucrose, BAP, and cefotaxime), the media turned red after 2 days. What could be the reason for this, and how can I resolve it?

The structure of the energy mix of various energy sources is determined by a number of factors. On the one hand, these are historical factors, technological, geographic, natural, economic conditions, etc. On the other hand, these are the determinants arising from a certain adopted energy policy, including taking into account the implementation of the goals of sustainable development, the principles of green transformation of the energy sector, social climate and environmental responsibility, and taking care of prospective future energy security. Taking into account the aforementioned determinants, there is a non-uniform structure of the energy mix of various energy sources in different countries. Taking into account the mentioned energy policy issues, the structure of the energy mix of various energy sources should be constructed in such a way that the national energy system, on the one hand, is characterized by independence from various factors and a high level of energy security, and, on the other hand, should also be in line with the green transformation of the economy and the implementation of sustainable development goals. In Poland in recent years, in terms of renewable energy sources, photovoltaic was the most significant in the structure of the share of installed capacity. Wind power came second, hydroelectric power was third, followed by biomass and biogas power plants. Unfortunately, still more than 70 percent of electricity and even more thermal energy is generated in Poland in conventional thermal power plants powered by coal or lignite. Successively from year to year, as part of the progressive green transformation of the energy industry, the share of various types of renewable energy sources in the energy mix of energy sources is steadily increasing.

I described the key issues of the green transformation of the economy, including the green transformation of the energy sector, in my article below:

I invite you to discuss this important topic for the future of the planet's biosphere and climate.

In view of the above, I address the following question to the esteemed community of scientists and researchers:

What do you think about this topic?

What is your opinion on this issue?

Please answer,

I invite everyone to join the discussion,

Thank you very much,

Best regards,

Dariusz Prokopowicz

I have a plasmid (~9.3kb) with a small 20 nt insert, which I created using the Q5 Site-Directed Mutagenesis Kit via PCR. Before transformation, I obtained a clear band at 9.3 kb.

I picked up a single colony into competent E. coli and let it grow overnight. The next day, I performed a MiniPrep to extract the DNA and quantified the concentration using NanoDrop. The DNA concentration was around 200 ng/µl, with an A260/A280 ratio between 1.9 and 2.0.

I then analyzed the plasmid on a 1% agarose gel after linearizing it with a restriction enzyme (RE) for 2 hours at room temperature. I expected to see a clear band around 9.3 kb.

However, I observed multiple bands in the gel, which has left me confused about the results. Do you have any suggestions or ideas about this data?

Please share your thoughts!

Thank you, and I wish you success in your life science research.

I am working on yeast transformation of a calcium channel. I performed twice transformation and no colony was observed after 3 days. The transformation protocol works well before. Is this gene toxic to yeast for the calcium channel activity? If so, How to achieve the transformation?

Can I apply heat shock transformation method for Gram positive competent Bacteria?

I need to transform a plasmid of 10 kbp in Gram positive competent Bacterial. Right now, I do not have electroporation facility. Is there any successful record of heat shock transformation in Gram positive Bacteria? If yes, does it require any modifications in procedure?

Dear Collegues,

Frank R. Tangherlini turned 100 years March this year.

His remarkable achievement is derivation of linear transformations known by his name as an alternative to Lorentz Transformation, which implement absolute simultaneity. He is also behind the "Tangherlini metric," an exact solution to Einstein's field equations that generalizes the Schwarzschild metric to higher dimensions. This work has important implications for our understanding of black holes and general relativity as well as fundamental meaning of time.

However, I've noticed that Tangherlini's biography is woefully underrepresented on the English version of Wikipedia, with only a single sentence mentioning his name. In contrast, the French Wikipedia has a substantive article (https://fr.wikipedia.org/wiki/Frank_Robert_Tangherlini) providing basic details and related links.

I believe Tangherlini's centennial milestone and his significant scientific legacy deserve more prominent recognition on the world's largest online encyclopedia. Could some of you with strong English writing skills and a passion for science history consider contributing to an expanded and informative English Wikipedia article on Frank R. Tangherlini?

This would be a wonderful way to give this pioneering physicist the recognition he deserves, especially on the occasion of his 100th birthday. Let's work together to correct this potential oversight and ensure Tangherlini's important work is properly documented for future generations in the English speakers global online resource.

Thank you for your consideration. I look forward to seeing what we can accomplish as a team. Please keep me posted

General commmentary on Tangherlini's achievements:

I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my regression analyses, I encountered the following problem:

Thank you in advance for your help.

Hello!!! I want to implement the Swerling characteristics functions (CF) directly in MATLAB without using its Fourier integral pairs...the Swerling CFs are actually Laplace Transform of the signal PDF. is there anyway we can directly measure the probability of detection from Swerling0 CF...(all other parameters provided like SNR, Number of pulses)

Example: the swerling0 CF is exp[-N*x*(s/s+1)]/(s+1)^N (x is SNR, N is number of pulses which in slow fluctuation case will be 1). how can we handle "s" in this equation it is transformation variable. it has real and imaginary parts both...Any one can help me directly implementing this CF in MATLAB...I have to make a Confluent Hypergeometric function from this CF

RBV and SBV

I'm having trouble with Bacillus velezensis FZB42 (Bacillus amyloliquefaciens FZB42) transformation. I've tried electro-transformation using the MicroPulser Electroporator (which only allows voltage adjustment) with integrative plasmid, but I haven't gotten any colonies. Is there anyone who has used the MicroPulser Electroporator for Bacillus FZB42 transformation? Any advice on what might be going wrong?

How to write a academic proposal concerning the Transformation of language identity among the Gurung community?

Hi, I have some experiments where different nutrients/fertilisers (including but not limited to N) were applied to plants.

Unsurprisingly the data produced is bimodal. The populations are N-applied and N-not-applied.

Is it more robust to separate the populations and do an ANOVA on them separately or to transform the data using something like log/square-root/Boxcox/GMM? If transformation is the answer what kind of transformation is most appropriate?

Thanks very much.

During the heavy warm rolling process, austenite undergoes dynamic recrystallization (either DDRX or CDRX), and then transforms into martensite upon quenching. Is there a way to distinguish from the final martensitic structure in EBSD which parts have transformed from recrystallized austenite? Among the points that are particularly important and challenging for me are:

1. Recrystallization occurs during the processing, and the subsequent martensitic phase transformation through the K-S relationship can affect the judgment of grain orientation (for instance, FCC DRX may produce Σ3 twin boundaries, and the variants after martensitic transformation might also have Σ3 grain boundaries).

2. The nucleation of martensite in the austenite with strong plastic deformation is influenced by the strain gradient and may nucleate along the path that minimizes energy most rapidly. The nucleation of martensite during the phase transformation might affect the degree of intragranular distortion, and the original austenite intragranular distortion may not be accurately reflected by the martensite. Therefore, using common methods such as GROD (Grain Reference Orientation Deviation) or GOS (Grain Orientation Spread) to determine recrystallization seems incorrect.

(The above issues mainly concern grains with sizes below 500 nm); my understanding may not be comprehensive or have errors, are there any relevant studies to recommend?

Hi all,

I am facing a challenge with transforming some variables for logarithmic analysis in my ARDL model. These variables contain negative values, which complicates standard logarithmic transformation. I applied a log transformation with offset: Ln(X)=sign(x)*ln((abs(x)+1)

Thanks for your help!

I would like to set up a measuring device to determine the transformation temperatures (M_s, A_s) of austenite-martensite (in Cu-Al-Mn). In the past, a self-built system was used for this purpose, which has the following general structure.

The acoustic emission was recorded using a piezo wideband sensor (100-1000 kHz) and then transmitted to a measuring computer (with measurement card and associated analysis software) via a preamplifier. Heating and cooling was realized by Peltier elements operated by a laboratory power supply. The temperature was recorded using thermocouples.

Are there any ready-to-use measuring systems for such investigations or does anyone have any tips on what to look out for when setting up such a system?

My specimen will be small wires with a diameter of approx. 2mm.

Can the pACYC177 plasmid be used for transformation and gene complementation in Pseudomonas aeruginosa?

I've been doing transformation several times with DH5. I got overgrowth/bacterial lawn growing on my plates twice, and when I transform for the 3rd time, there's no colonies formed. I'm using heat shock method with chemically synthesized plasmid and ampicillin as the marker. The plates i'm using is only several days old and i cover it with amp before plating the transformants. what should i do to increase my TE?

I wonder if anyone can help me locate any references discussing the hee or hte phenotypes attributed to some e coli cells. Are these able to be tied to a genetic mutation? I find suppliers advertising comparative rates of transformation with other commonly used cells but no papers that provide any more information.

Thank you

Hello.

I am currently working on transformation of Enterococcus faecalis to do some knockout experiments. Specifically, I want to remove a gene encoding an enzyme that is useful for probiotic application in order to confirm its function.

Is there any established protocol for heat-shock transformation of Enterococcus faecalis with high transformation efficiency? Currently, electroporation is not an option so I am limited to heat-shock transformation only.

I hope you can help. Thank you!

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To do gibson assembly cloning can I use DH5alpha strain of E. coli. for transformation?

I need to know about box - cox transformation. Sometimes, other transformations don't make the data normal. Can box-cox solve the problem?

we did gene transformation to tomato plant by agrobacterium tumefaciens. but when we examined the obtained explants, PCR results were sometimes positive and sometimes negative when we examined the DNA sequence located between the RB and LB part of the binary vector. We believe that sometimes positive results are obtained because Agrobaterium remains in the plant body. How can it be determined that the T-DNA part has been integrated into the plant genome? No growth of negative control plants was observed in selective medium.

We performed the transformation using the following method.

Agrobacterium tumefaciens-Mediated Transformation of Tomato

Joyce Van Eck 1 2, Patricia Keen 3, Michelle Tjahjadi 3

Affiliations expand

My vector size 5.1kb and insert size is 4kb. I performed double digestion of vector and insert, ran them on the gel and then extracted them from the gel. 260/280 ratio was good. Then I did ligation using T4 ligation at 4C and transformed into E. coli DH5a. After 24hr, I did not see any colony. I left the plates for another 2 more days in the incubator. Now I see some tiny colonies. My question is -

1) Is there any way to check whether ligation or transformation is the problem?

2) The OD600 of competent cells was about 0.9. Would that be an issue?

3)Are the tiny colonies after 3 days transformed colonies or not?

4) Any suggestion for successful cloning with my vector and insert sizes?

Thanks in advance

Hi All,

This may sound like a stupid question, but I have a yeast transformation protocol that I am trying to modify for automation. Unfortunately, our automated equipment is not under any sort of closed environment but is open to the lab. Has anyone been able to get away with doing a yeast transformation out in the open without using a biosafety hood? I typically use tetracycline during the final transformation so I have avoided contamination so far from bacteria, but I still keep my transformed samples under the hood of course mainly with fears of mold contamination which we have encountered in the past. Does anyone think that it would pose a problem to attempt a full transformation using automation outside the hood? what things might I need to consider before I attempt it?

Thanks in advance.

Are Lorentz transformations a paradox?

The modern proof of Lorentz transformations makes you laugh and the modern proof of mass energy equivalence E=mc^2 keeps you laughing.

So what ?

I am trying to integrate a 11 kb DNA, linearized with AvrII, at GAP locus in Pichia pastoris genome. I am using electroporation for the same. After transformation, I am observing colonies after 2-3 days but in test plate as well as negative control. I have tried using different concentrations of the antibiotic selection marker as well (100, 200, 400 ug/ml) but I am facing the same problem. Can anyone please suggest any solution for this?

I am working on my dissertation on Analysis of rural transformation in Ethiopia: Extent, effect and challenges. I want to review theoretical and conceptual framework that can guide my study.

i am working with gene editing in bell pepper. i have done agro transformation by floral dip method. (LBA4404 carrying plasmid, at final pellet dissolved in 5% sucrose containing 0.02% silwet ). i used different time for dipping like 5sec, 10sec, 30sec, 1min. but 3-4 days after the transformation, flowers flown down by truing the flower stem yellow color. kindly suggest, what can be the reason of the cause or how can resolve the problem.

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This discussion explores the inherent limitations of the Lorentz transformation, a cornerstone of special relativity, particularly concerning its treatment of acceleration. While the Lorentz transformation adeptly describes relativistic effects such as time dilation, length contraction, and mass increase, it falls short in directly accommodating acceleration. This discrepancy becomes pronounced when the velocity-dependent Lorentz transformation fails to reconcile velocities between rest and inertial frames without the presence of acceleration, thus highlighting a significant gap in its applicability.

The discussion delves into the historical context of the Lorentz transformation, acknowledging its development by Mr. Lorentz and its status as a final form in science. However, it also underscores the expectation for accurate physics within its framework, especially considering the pre-existence of the concept of acceleration predating Mr. Lorentz. This expectation includes honouring Isaac Newton's second law, which governs the dynamics of accelerated motion in classical mechanics.

While the scientific community initially accepted the Lorentz transformation without questioning its treatment of acceleration, there is now a growing recognition of the importance of integrating principles from classical mechanics, such as Newton's second law, to address these limitations. The discussion emphasizes the need for a more comprehensive theoretical framework that harmonizes the principles of classical mechanics and relativity, thereby offering a more unified and accurate depiction of physical phenomena.

The Lorentz factor (γ) becomes relevant when the object attains its desired velocity and is in motion relative to the observer. Initially, when both reference frames are at rest, the object's energetic state reflects its lack of motion, resulting in zero kinetic energy (KE). As the frames separate, the moving object undergoes acceleration until it reaches its desired velocity. At this stage, the object's energetic state reflects its motion, and it possesses kinetic energy (KE) due to its acceleration. This acceleration is not accounted for in the Lorentz factor (γ). Once the object reaches its desired velocity, its energetic state reflects its motion, and it possesses kinetic energy (KE) due to its velocity. The Lorentz factor (γ) and kinetic energy (KE) play significant roles in relativistic motion. However, the acceleration component is not considered in the Lorentz factor (γ).

This discussion delves into the intricate relationship between acceleration, inertial reference frames, and Relativistic Lorentz transformation. It scrutinizes how the necessity of different velocities for separated reference frames underscores the pivotal role of acceleration in achieving this transition. By integrating classical mechanics concepts like Newton's second law and Hooke's Law with relativistic physics theories, the discussion enriches our comprehension of motion in diverse reference frames.

The initial motion and separation of inertial reference frames are crucial for their physics, but once they separate, they must have different velocities, with the first frame's velocity (v₀) and the second frame's velocity (v₁) needing acceleration to achieve v₁ > v₀. This acceleration is essential in both classical mechanics and Relativistic Lorentz transformation. The Lorentz factor (γ) is a velocity-dependent factor that involves velocity-induced forces, affecting the behaviour of objects in motion. It is based on the equation E = KE + PE, where KE is treated as 'effective mass'. Piezoelectric materials can convert mechanical energy from vibrations, shocks, or stress into electrical energy, typically an alternating current (AC). This process involves force-mass conversion, where the force applied to the piezo actuator results in a deformation or displacement. The displacement ΔLɴ of the actuator is inversely proportional to the stiffness, highlighting the interplay between force, stiffness, and displacement in force-mass conversion.

what is isothermal?

how to work it?

explain the isothermal diagram?

and also explain diagram ?

I'm currently trying to capture a biosynthetic gene cluster using Transformation-Associated Recombination (TAR) in yeast. After identifying positive yeast clones, I extract, via an alkaline lysis method, the plasmid from yeast and electroporate it into E. coli DH10B.

However, I have not been able to find any positive hits upon cPCR on Eci clones despite testing about 100 colonies using the same diagnostic primers I used to identify the yeast positive clones. Then, on some of the E coli that I pick and miniprep, I consistently only see my original capture vector (with no gene cluster inserted).

The issue may lie in the yeast plasmid extraction, the transformation, or the plasmid isolation prep from E coli. I know that yeast plasmid extractions are hardly ever clean and tend to be "dirty," contaminated with yeast gDNA and other DNA it has inside due to the IPA precipitation required to perform the method. That tends to lead to poor transformation efficiency into E coli. But I feel like I'm stuck going in circles trying to bring the plasmid into E coli and isolating it. if anyone has ever worked with TAR and has experienced any troubleshooting at this stage of the process, I would appreciate insight. Or any advice on things I can try to better improve yeast plasmid extraction or transformation/isolation in E coli. Many thanks!

I have a phytochrome protein that takes PCB. The SAR is very low when i coexpress the phytochrome with the pigment producing enzymes. So usually we used to add PCB from external sources. However, our vendor stopped making them. Now i tried fresh transformation, and some other methods hoping to get better PCB levels in vivo. None of them are helping. Any suggestions?

Thank you

What are the key aspects, benefits, and downsides of System Dynamics if applied to business model innovation for digital transformation?

In the security proof of CVQKD, it requires that the protocol is invariant under unitary transformations in phase-space. How to make a protocol satisfy unitary operation invariance?

What transformative techniques are there in biology?

hi everyone,

i am trying to figure out why the gene inserted is not amplifying after cloning. I have inserted a mycobacterium-specific gene into pET 32a plasmid and transformed the ligation product with BL21 cells. after transformation, I have isolated the plasmid from the obtained clones and done restriction digestion with the desired restriction enzyme. i have got a positive result for this experiment by running the restriction digestion product in 1% agarose gel. hence i kind of confirmed that the cloning has worked. ionrder to re confirm it, i have done a colony PCR with the obtained transformant colony.but i have got no amplicon for the gene. i have also tried to amplify the gene from the isolated plasmid using the gene-specific primers,but that also gave a negative result. i have repeated these experiments for multiple time but each time am getting the same pattern of result. that is a positive result for restriction digestion of the isolated plasmid and negative result for the colony PCR as well as the plasmid PCR. what can be the possible reason behind this. also i tried to express the protein using iptg induction, that also resulted in negative result.

I am writing to let you know that Journal of E-Learning And Knowledge Society (SCOPUS Q2, SJR Q3) is currently welcoming submissions of original research to "VOLUME 20 | SPECIAL ISSUE CALL 2024" with title “Digital Transformation in Educational Research: Competencies, Resources and Challenges in the Context of ICT”. As the Guest Editor of this CfP, I hope you will consider this as an outlet for a future research paper.

As a result of these reflections, different questions arise.

• Are teachers digitally trained in research skills?

• What skills do teachers have to use digital resources developed for the research context?

• What factors influence the digital competencies of teachers in their research work?

• How do AI tools impact the digital skills of teachers in research work?

For this reason, we welcome studies that cover topics related to:

• digital skills in research work;

• analysis of the use of digital resources and AI tools for the research process;

• factors incident to the digital competencies associated with the research process;

• infrastructure of university institutions on digital resources for the research process.

This inquiry seeks to explore the transformative effects of the Direct-to-Consumer (D2C) business model on the e-commerce industry. It aims to understand how D2C strategies have altered consumer behavior, distribution channels, and performance indicators such as sales growth, customer acquisition costs, and retention rates. The focus is on identifying shifts in market trends, operational challenges, and the overall impact on the competitive landscape of e-commerce.

I have a plasmid with kanamycine antibiotic resistant gene and Bar as a marker gene. I need to transfer this plasmid into AGL-1 strain of agrobacterium tumefaciens. I faced a problem when I follow the protocol steps of transformation. I did not get bacterial colony even after 2-days of a LB media plate having Kan 50ug/ml.

Protocol steps

1- Take competent cells from -80 C.

2- Add 5ul of plasmid having conc. 50ng/ul in 100 ul of AGL-1 BACTERIA.

3- Keep on ice for 30 min.

4- put in liquid nitrogen for 5 min

5- keep on heat bath for 5 min.

6- keep on ice for 5-min again.

7- add 800ul of LB without antibiotic (Kan)

8- Shake for 2-hrs at 28 C.

9- spread on LB media plate with kan 50ug/ml. Keep these plates on 28 C for 2-days.

But did not get the bacterial colony.

These are the protocol steps which I followed. Anyone can guide me where I am doing mistake?

We think the short answer is most likely yes, he did.