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Tissue Mechanics - Science topic

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Dear sir,
I am going through your paper titled " Micromechanical modelling of skeletal muscles based on the finite element method " . You have mentioned force velocity characteristics with equation (numer 9 and plotted the graph (figure 4)).In that relation kc , ke are the curvature and d is the offset of the eccentric function.
My question is kc,ke , d same for all kind of muscles ? if not how to get the values of kc,ke , d values for different muscles?
Could you please reply me sir.
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To answer your question, the values of kc, ke, and d in the force-velocity relationship may not be the same for all types of muscles. The force-velocity relationship is influenced by several factors, including the muscle fiber type, the size and length of the muscle, and the contractile history of the muscle. Therefore, the parameters kc, ke, and d may vary depending on the specific muscle being modeled.
In order to determine the values of kc, ke, and d for different muscles, experimental data is typically needed. These parameters can be determined by fitting the force-velocity relationship to experimental data obtained from measurements of muscle force and shortening velocity under different conditions, such as varying levels of activation or different types of contractions. Alternatively, these parameters can be estimated using a combination of experimental and modeling techniques, such as inverse modeling or parameter optimization.
It is also important to note that the force-velocity relationship is often simplified in muscle models, and may not fully capture the complex interactions that occur within the muscle during contraction. Therefore, while the parameters kc, ke, and d can provide useful insights into the behavior of skeletal muscles, their values should be interpreted with caution and should be validated against experimental data whenever possible.
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I am trying to find a reference for elemental and molecular composition of different tissues.The only ones I could find are from 1970s. NMR, MRI or mass spectrometry studies? 
In a way it is so basic knowledge it's hard to find good references.
Thank you!
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Dear all, I have the same problem. The only data I can find regarding the molecular composition of different body tissues is from the 1980s or older. It´s seems to be really basic knowledge of high importance (e.g. for pharmacokinetic modelling purposes). Modern analytical techniques should allow more acurate, maybe hyphenated, determination....So does anybody know a more recent publication which covers this topic? Thanks in regard
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I'm developing with my team a bioreactor intended for soft tissues' mechanical stimulation, we were interested in a SFF method for its realization. Are there any polymers suitable (medical grade) for the production of a bioreactor culture chamber and parts?
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Can you further explain your question?
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Ultrasound frequency waves may affect developing tissue mechanically
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Definitely no
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Bone fracture is the most common effect in osteoporosis. one the reason is the impact of osteoporosis on the density of the cortical bone. So I'm looking for the role of the density disorder which is caused by osteoporosis in bone fracture.
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Hi Dear Sophie,
Thank u very much. I'll chock out it for sure.
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It's clear, osteoporosis has a direct impact on bone density. How can I figure out the manner of this effect in spongy and cortical bone, separately?
On the other hand, I'd check out how this effect of osteoporosis changes the orientation of bone blades?
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Dear Mehrshad,
on elf the best reviews in the field of cortical and trabecular bone adaptation due to osteoporosis is the following one
Kind regards,
Ramona Ritzmann
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I am going to measure flow using ultrasound.
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' Hi , Not fully , but yes there is .
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I stained the human tissues with DAB and I have to see the effect of another protein so I stained with alkaline phosphatase. I believe by go through xylene and alcohol,  DAB and hematoxylin will get rid off but not done well. 
I have one slide only so I have to strip both DAB and alkaline Phosphatase along with counter staining of Hematoxylin to do the one protein stain with alkaline phosphatase. Please let me know if anyone know any protocol to strip both DAB and alkaline Phosphatase along with counter staining of Hematoxylin.?
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That is my understanding as well. DAB is a precipitate, a very large one. Even if you could somehow rehydrate the tissue by first soaking in xylene and then in alcohol and then finally water (the reverse of the dehydration protocol) I do not think you will be able to resolubilize the DAB. Not in any way that won't completely destroy your tissue anyways.
Sorry for the bad news, but maybe try talking to a representative from Vector Labs. They make DAB and AP kits, they might be able to help you with an answer.
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Good evening, ladies and gentleman.
Could you bring to me a little of your  wise scientific expertise?
I was wondering whether if all tissues (in particular: CNS, splenic and liver) are equally susceptible to IL-1beta induced COX2 expression.
Anyvbody out there? :)
Thank you a lot and good Science to all
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I have no experiance
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I have a cardiac fibroblast, but it seems that they grow slowly. I am using DMEM supplemented with L-glutamine, FBS, and PS.
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I add up to 10ng/ml bFGF (also called FGF2) to DMEM supplemented with 10% FCS & Glutamine. The cells grow very well under these conditions. I used from 2 different companies (Peprotech and then a cheaper one, prospec) and both work fine!
All the best!
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After trypsin, the cardiac fibroblasts did not attach well (less than 5 out of 100 were attached on the second day after trypsin). This also happened for cells taken from liquid nitrogen. I can see the cells are floating.
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Thank Johanna and Torry.
I used Less than 4 mins for trypsin with concentraiton of 0.25%.
I tried to remove DMSO on the second day and immediately by pellet after thaw, both gave me similar results on the cell attachment.
And yes, the floating cells are dead at >70% (via trypan blue).
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I starting some work on scaffolds for tissue engineering using biodegradable materials. At the moment, I'm doing some preliminary experiments with SU-8, which is highly suitable for soft-lithography but lacks biodegradability. I'm looking for a suitable substitute material for long term use. Does anyone have any suggestions?
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