Ticks - Science topic
Blood-sucking acarid parasites of the order Ixodida comprising two families: the softbacked ticks (ARGASIDAE) and hardbacked ticks (IXODIDAE). Ticks are larger than their relatives, the MITES. They penetrate the skin of their host by means of highly specialized, hooked mouth parts and feed on its blood. Ticks attack all groups of terrestrial vertebrates. In humans they are responsible for many TICK-BORNE DISEASES, including the transmission of ROCKY MOUNTAIN SPOTTED FEVER; TULAREMIA; BABESIOSIS; AFRICAN SWINE FEVER; and RELAPSING FEVER. (From Barnes, Invertebrate Zoology, 5th ed, pp543-44)
Questions related to Ticks
I need a diagnostic key that describes all the ticks that carry diseases between humans and animals.
I have a set of positive COVID-19 cases by district in a state. I want to use agent based modelling to make the infected cases move around the state and count the number of infected, number of healthy and number of immune. I have a set of code which the number of population is random and not based on the location. Tried to watch youtube for the tutorial but could not find one which really helps. the code is as below. I want to insert the map of the state, number of population for each district and number of infected in Netlogo.
create-turtles 200 [
setxy random-xcor random-ycor
set shape "person"
set color pink
set size 1.0
set illness 0
ask n-of starting-number-infected turtles [
set color yellow
set shape "X"
set size 1.0
ask turtles [
rt random 360
ask turtles [
if color = yellow [
ask turtles in-radius infect-distance [
if random-float 200 < infect-chance [
if color = pink [
set color yellow
set shape "X"
ask turtles [
if color = yellow [
set illness illness + 1
if illness > infectious-period [
set color white
set shape "circle"
set size 1.0
set illness 0
ask turtles [
if color = white and waning-immunity < random-float 100 [
set color pink
set shape "person"
If you have specific experimental data on ticks that parasitize on the body surface of birds, we would appreciate it if you could share the data.(ex: Prevalence Infested Birds, Abundance of Ticks. etc)
For adult immersion test what is average weight of engorged females of Rhipicephalus microplus. And also average weight of eggs.. Or what percentage of the engorged tick weight will be egg weight
It is a known fact that ticks can migrate to other continents by parasitizing on the body surface of migratory birds. There are some specific influencing factors that affect this, and I would like to know the interrelationship between them.
I found this approach -the per-node approach- in a paper recently published, in which the author tried to describe a new way to tick a behaviour tree instead of using the conventional approach, in order to evolve a behaviour for an agent (game agent) using genetic programming.
I would like to discuss this approach and how it works deeply.
If anyone could share any idea about this, it will be more productive.
I have a technical question of AMOS when conducting a confirmatory factor analysis. I've drawn the pattern matrix based on the result of principal component analysis in the previous step (see attached the picture). I've also ticked all the necessary boxes of Output in the 'Properties analysis'. The software runs but no factor loading was shown after I clicked 'Calculate Estimate'. I am not sure what I have done wrong (or what I haven't done).
By the way, this is the fresh data file. It wasn't used in the previous principal component analysis.
Looking forward to hearing from anyone who's had AMOS experience!
Currently in our lab., we have a NANOJECT II, an injection device from Drummond Scientific Company used to perform the dsRNA injection in ticks. Thanks in advance !
I am a graduate student engaged in research on ticks and tick-borne diseases.
Currently, parasitic ticks are difficult to collect, and free ticks cannot be collected at all.
I have tried the dry ice method, animal trap method, flagging method, and dragging method, but no questing ticks have been collected. The only tick samples are collected from the surface of animals, but the method of collecting ticks from the surface of animals is inefficient. (0-10 ticks/animal), a group of animals often does not even collect even one.
Do you have any tips for collecting parasitic ticks and questing ticks? Or what areas have a higher chance of stray ticks and parasitic ticks?
Hello! I would like your help with a mite issue in the lab. These mites in the photo appeared yesterday on flea rearing petri dishes where there were flea larvae and the substrate (sand and dry bloodmeal). They were able to escape the dishes and are now crawling around the incubator. I took the pics by placing a drop of water in a slide - 1st question: can you please share a better, but easy mounting method? I have an acarina pictorial key on hands, but my slides are poor quality so I can’t see all the details I need to see to id.
2nd question: If you can identify these mites from my photos, what are they? Knowing the species will help us understand the origin of problem: there are dogs, cats but also there’s access to feral mice and birds.
3rd question: best method to kill these without killing other ectos - fleas and ticks? I cannot warm up the incubator too high to cook them, I wish.
Thanks for reading all this and for your help! Pics to follow Flavia Ferrari
This video is explaining it. https://youtu.be/cUyd0kJodi4
#customticklabels #originpro #sayphysics
How to add custom tick labels at specific values in origin
My question is;
What are the various reasons behind unawareness as per the consumer?
I am trying to find the impact of one reason on another.
Which method of analysis can help to derive an impactful answer?
I use OriginPro9 crack, but I noticed recently I can't add Tick Labels on the graph. I have tried resetting the Tick label display but it didn't work.
Please, kindly suggest how I can reset the tick label to display or assist with a downloading link for another crack version of Origin software.
Although I check that the flow accumulation tick is OK in display options, but still there are no streams on the map...
How to fix this in order to continue watershed delineation?
Does anyone have a PDF identification key for hard ticks in the European regions?
In my g power ap which linear multiple reg should I tick? One says R squared deviation from zero and the other says R squared increase. I'll be doing a Hierarchical multiple regression analysis with a health beliefs questionnaire as my dependent variable and I'll have several independant variables (categorical and continuous) such as sex, education level, and the response to several different questions, and scores on a characteristics scale.
We are looking Leishmania and Rickettsia in tissues of roadkill in Ecuador. But, we don´t have ADN of Rickettsia for using it like a positive control. Please, someone would help us with this ADN?
My name is Sandra Enríquez, I am entomologist and am working with ticks of Ecuador.
Being in different biotopes, ornithologists find nests of wild birds, catch them for study. Birds are carriers of ticks, which in turn are carriers of viruses. In addition to itching, they do not harm humans, but through clothing or other things, feed can get onto poultry. Therefore, I would like to know modern methods and measures to combat ticks in wild birds caught in the forest?
I have no access to the BSK II medium and want to try in vivo culture of Lyme borreliosis agents. I appreciate any guidance or reliable references.
- I have conducted a survey which includes two groups (1-Normal people, 2-Deaf and Dumb), and the questionnaire had a category of questions with 4 different options and to answer only tick one. I have calculated the percentage of responses for both groups separately. But, I want to compare the response of the two study groups. Can anyone suggest which statistical test can be used?
- Example of Question: Why do you use emojis? and the options are as 1- I don't use them, to express emotions, 3-To make the text easier to understand, 4- Because it is quicker than writing the actual text.
- Any help will be appreciated.
which software is easy and best to use for SEM, scanning electron microscopy images analysis ..
such as for stomata area. trichome length. trichome tickness. fruit peel different layers.
We are looking for serum against saliva of Ticks (Ixodes) obtained from bitten rabbits (after 24 or longer period of feeding ticks).
We need it for testing some of our prediction models for epitopes of primary response to a mixed pool of antigens.
Would highly appreciate any help from labs involved in Tick research :)
How can a clock inside a spherical shell "know" that it should tick slowly? Unlike Newton's action-at-a-distance theory of gravity (though even Newton himself had reservations about this), Einstein's General Relativity is a FIELD theory. Yet there is NO gravitational FIELD inside the shell, and spacetime inside the shell is Minkowskian. Furthermore, let the shell radially contract (expand). The gravitational POTENTIAL inside the shell then becomes more (less) strongly negative, so the clock must then tick more (less) slowly. Yet there still is NO gravitational FIELD inside the shell and spacetime inside the shell still remains Minkowskian. By Birkhoff's Theorem, even while the shell radially contracts or expands (not merely before and after the radial contraction or expansion) there is NO gravitational FIELD inside the shell (also no gravitational wave generation) and spacetime inside the shell still remains Minkowskian. So with NO gravitational FIELD to interact with and NO change of the metric coefficients from their Minkowskian values, how does a clock inside the shell "know" that it must tick slowly, even though the gravitational POTENTIAL inside the shell is negative? How does it "know" that the gravitational POTENTIAL has become more (less) strongly negative after a radial contraction (expansion) of the shell, and hence that it must then tick more (less) slowly --- even though the field always remains zero and the metric coefficients always remain Minkowskian?
Hye all, hope all of you doing fine today.
In my case for viscosity that dependent on temperature case, supposedly I need to tick the energy equation and laminar-viscous heating, unfortunately, the simulation will be faced with an error. But when I untick the viscous heating the system can be run and get the simulation result. As per attachment location for energy and laminar-viscous in ANSYS FLUENT, my UDF for viscosity dependent temperature case and error that appears when i tick the viscous heating.Thanks..any idea guys?
Usually it's the stereo microscope that we use to observe tick morphology; but my question is why the conventional light/optical microscope is not used for this purpose and if possible, is there a special method for it?
I want to express a protein found in the midgut of one the ticks and save time by using genomic DNA instead of cDNA library.
I am working with miRNAs from the tick's salivary gland, midgut, and saliva to study the involvement of these miRNAs in host-vector interaction for successful blood feeding. Do you know any stable reference genes which I can use for miRNA qPCR data normalization from ticks? I have found EF-1 and Beta Actin genes as the most frequently used reference genes in articles but I will appreciate it if anyone knows the most stable expressed miRNA/other small non-coding RNA which can be used as reference gene for miRNA expression study in ticks.
Thank you very much in advance and looking forward to your information and suggestions.
I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and didn't run?
Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known.
However, we have two concepts, static instruction, and dynamic instruction. It is possible to have a program with ten static instructions, that execute several times, ten million dynamic instructions, for the same program.
Is it possible to set a break-point at a specific dynamic instruction? For example, in some computer architecture simulators, it is possible to rise a trap at a specific clock cycle, or certain tick, like in Gem5.
I am planning to perform the Larval Packet Test (LPT) for my test. I need some tips/advice from the expert before I started to conduct it. This is due to the constraint I faced just to transfer a few larvae from one container to another. It makes me feel that is impossible to transfer 100 larvae to a small packet, neatly contain in it without any escape during and after transfer.
Hi, I want to extract DNA from ticks (to detect Haemoparasite like Babesia, Theileria, ).
What is the best method to crush ticks?
Which kit gives a better result for isolation and identification of Haemoparasite
Please suggest to me general universal primer paper as well if possible.
Looking forward to hearing from your advice
Hi all Scientists;
I have similar question. Can you please help me in selecting unique/distinct peptides in case of LC-MS/MS analysis?
I have done salivary gland proteomes for ticks and run it on Protein pilot and choose the proteins with at least 2 unique peptides at 95% confidence interval, however; there are some peptides which apparently showing that they have two distinct peptides but in real these both peptides have the similar sequence of amino acids. So Can you guys help me how to deal with this? Thanks in Anticipation
I recently dual booted my new lenovo ideapad S540 with Linux mint and installed anaconda in order to use jupyter notebook. I used some old data to plot with some old ipynb notebook, it shows the wrong formatting of the ticks. The same code works on other macbook, but on this machine, it shows really horrible formatting. I tried to search google but I could not find anything. This seems to be the problem with only plotting the data which I have in a file, while if I generate data in the notebook itself with (let's say linspace), it plots just fine.
import numpy as np import matplotlib.pyplot as plt f = open('data.dat','r') line = f.read() x = line.split() f.close() time = np.linspace(0,10**5,2000) plt.plot(time,x,lw=1) plt.show()
Please see this figure: It seems there are ticks for all the points, I have plotted.
I tried everything, I could, but I have no idea how to fix this. I even uninstalled anaconda and reinstalled it, removed the linux mint and reinstalled it and even changed it to ubuntu. Any suggestions are welcomed.
I have submitted a paper in a journal and the reviewers reply to me with this. Can anyone guide me on this how to solve it
Thanks in advance
Figure 4: (i) the image presented in panel (a) shows clear signs of measurements artifacts; (ii) Pale grey does not reproduce well; (iii) the labels presented on the axes of the images are not well aligned in the tick marks .; (iv) Verify the y- and x-axis scales.
I have tried several DNA extraction protocols to extract DNA from tick tissue samples (Promega tissue kit, QIAGEN tissue kit and phenol-chloroform DNA extraction method have used). But I didn't get results from any of these methods. I assume that it would be due to incomplete lysis of tissue samples. I would be so grateful if anyone can drop an answer to my question or any suggestion regarding this. I am looking for a best tissue lysis buffer for tick DNA extraction.
I am trying to model a disc in 2D as attached here. Because the disc is complete circular object, I tried to tie side A to B using MPC tie constraints. However, Abaqus gives several warnings for the side nodes that “***WARNING: SLAVE NODE 153 INSTANCE FRICTION-DISC-1 WILL NOT BE TIED TO THE MASTER SURFACE ASSEMBLY_FSIDE1_CNS_. THE DISTANCE FROM THE MASTER SURFACE IS GREATER THAN THE POSITION TOLERANCE VALUE”.
I decided to specify a position tolerance value but Abaqus abort the work saying ***NOTES: DISTORTED ISOPARAMETRIC ELEMENTS: ANGLE BETWEEN ISOPARAMETRIC LINES IS LESS THAN 45 DEGREES OR GREATER THAN 135 DEGREES. Nonetheless, when I untick “ Adjust save surface initial position", Abaqus runs successfully with several warnings “ ***WARNING: *TIE BETWEEN SURFACE PAIR (ASSEMBLY_DSIDE2_CNS_, ASSEMBLY_DSIDE1_CNS_) MAY RESULT IN UNREALISTIC DEFORMATION BETWEEN THE SURFACES OR LARGE STRESSES BECAUSE THE MASTER IS NODE-BASED AND NO ADJUSTMENT IS SPECIFIED. DEFINE A SURFACE-BASED MASTER SURFACE OR ADJUST=YES TO AVOID THIS PROBLEM. If I tie using surface to surface, I get the same warning and if I tick "adjust ", Abaqus abort because I have specified a tolerance position.
I have also tried to use an input file and tied the side nodes directly using the tie command, yet I get the same errors. Please any suggestions on how to handle this kind of problem.
I have ordered customized primers for ticks candidate miRNAs as these miRNAs are not available in any company. I have used a miRNA-specific MystiCq cDNA synthesis kit for cDNA synthesis and it has quite a good concentration. I have made qPCR with Maxima Sybr green with 10 fold dilution for efficiency curve which gave not a good result for 3 miRNAs (Ct 35-46, maximum undetermined), however with one miRNA average ct 26.66, 28.84, 35.31, 38.57, 41.50 and efficiency curve was 79.39. I have also adjusted annealing temperature using tm calculator from Thermo Scientific. Can anyone suggest what I could do to get a better efficiency curve? Should I also really need to change qPCR kit specific just for miRNA to get consistently acceptable ct and efficiency curve results for all miRNAs?
Thank you in advance for your answers.
Identification of ticks morphologically is an effective way to identify the species or genera of ticks and other ectoparasite infestations of an animal. However, there are many methods used today to mount, prepare and observe these specimens. This discussion is expecting to share the known methods for specimen investigation and to critically assess them.
I am facing some issues with Sybre green qPCR results. I have isolated total RNA from different organs of ticks using the Trizol RNA isolation kit. After cDNA preparation with a random primer, I used specific miRNA primers for qPCR. I have got undetermined values in almost all 4 candidate miRNAs for my samples from the midgut, salivary gland, and saliva. I also did qPCR for elongation factor (Endogenous control) and CT values were between 26-35. What could be wrong and what I can do to solve the issue? Do I need to change the method to isolated miRNA instead of total RNA? I have chosen the Purelink miRNA isolation kit as its already available in the lab. Do you think changing the isolation method will help? Does anyone have experience with a similar issue?
Thank you in advance for your valuable suggestions.
Is there any study published and demonstrated which indicates the presence of the agent of rickettsioses? in other arthropods not hematophage.
I am trying to increase the space (pitch) between the maximum and minimum values, which is indicated by red colour in the following graph. Although the difference between both values are quite closer to each other.
Waiting for your earliest answer
Does anyone know a fast and simple method (apart of using tweezers) to sample ectoparasites (especially bat flies, Nycteribiidae, but also mites and ticks) on small bats (15 g)? We release the bats after sampling.
My questionnaire consists of 18 MCQs, each with one correct answer and 3 incorrect answers. I'm measuring participant scores on the questionnaire before and after watching a video in two seperate groups.
From what I've read Cronbach's Alpha is used to test scaled data (i.e. Likert scales) for reliability, so I'm unsure as to whether its appropriate for my questionnaire.
Can I also use it on my questionnaire, or is there an alternative more appropriate for my data?
If the answer is yes I can use it, do I analyse it exactly the same in SPSS as I would scaled data? i.e.: Analyze> Scale> Reliability Analysis, all questions into the 'items' box, tick descriptive statistics options 'item' 'scale' 'scale if item deleted' and 'correlations' in inter-item options?
Thank you in advance!
I will like to determine the level of various reactive oxygen species produced from ticks as part of an experiment. Considering the unstable nature of different ROS and their ability to dissociate within a short period, what is the best approach to get a good insight into how much ROS is been produced. Thanks
I have spent literally 5 hours trying to do this without success and I'm ready to throw my laptop or the window so if anyone could help me I'd love you forever!
I have 400 questionnaire responses for A level and GCSE students of different ethnic groups. I asked them to write down three words to describe geography. All responses are in a single data set. They had to tick their ethnic group, whether they were doing GCSE or A level. Each student is a row and the columns are ethnic group (Asian, black or white), level of study (GCSE or A level) and the three words.
All I want to do is create a word cloud for each of the following groups:
Asian A level students
Asian GCSE students
Black A level students
Black GCSE students
White A level students
White GCSE students
Can I do this in Nvivo?? If so, hooowww?! 😭
I am using Origin lab pro version 9. I am facing a problem with scale. Whenever I adjust scale then scale disappears even in tick label show button is ticked.
How credible are the test results for COVID-19? What are the False Negative rates of the testing kits used currently? Is it another ticking time bomb?
I am doing a 2x2 test in SPSS and one of the cells has a number less than 5. I have gone into descriptive statistics and ticked exact test as directed but the output does not show a number for the Fisher's exact test. It only has the same results as when exact is not ticked. Does anyone know why this may be?
So i need to do a quantification of Theileria annulata and Anaplasma marginale from tick DNA samples. But i am a bit confused when it comes to deciding on using a house keeping gene as most studies I have seen on these said pathogens did not use any.
I am searching an advice on how long a tick could be find always sticked (not moving on freely) on an animal after its dead?
I think that this is depending of the age of the tick, and the volume of blood they are still in need.
But is it possible to find some ticks still hooked after 3 days, 7 days, Less or more???
I wonder this question especially for big mammals like elephant, but data from other taxa are welcome
All papers or experiemental data are welcome.
Thank you for your suggestions
I am a Stata newbie and am trying to clean the data set of a survey which I recently conducted using LimeSurvey. One of the questions involved a 19 part psychometric test (Basic Psychological Needs satisfaction scale), with each respondent having to tick one of the following responses for each question:
1 = Not at all true
4 = Somewhat true
7 = Very true
The response data arrived into Stata in string form so I destringed it using
encode BPN_1, generate (BPN_1_d)
encode BPN_2, generate (BPN_2_d)
etc for all 21 questions in the scale
While the desstringed version of the response data (i.e. BPN_1_d etc) looks fine on the surface i.e still shows 1= Not at all true or 3 or 5 etc in line with the original data entry, when I click on each cell, I can see from the bar at the top of the page that as part of the destringing process Stata has 'recoded' all the original responses using its own (alphabetical??) system so that an original 1 response is coded by Stata as a 2 etc.
I was planning to fix this by going either of the following routes:
label define BPN_order
label values BPN_1_d BPN_order
replace BPN_1_d = 1 if BPN_1_d == 5 | Burnout1_d == "1: Strongly Agree"
but the problem is that Stata appears to have recoded a 3 response as a 4 for some of the 19 sub-questions, as a 5 for others and a 9 for others i.e. there doesn't appear to be any consistency as to how Stata has recoded the original responses when destringing.
Has this happened to anyone before and does anyone have any suggestions as to how I might overcome this problem or maybe de-string the original data in a fashion that might avoid it?
We wish to detect the presence of TBEV in ticks using a sandwich immunoassay using a viral protein as target. An envelope protein or a non-structural protein expressed (?) in the tick? Any suggestions welcome
I'm writing a paper where I'm asking a question with multiple choice where the respondent can tick multiple answers, as the example below. I'm also asking this question twice, in relation to a human and a robot to see if there are any differences.
I was wondering what the best data analysis method would be to apply here? my thinking was a multiple regression where I assign the number of answers to each category and run against the two null hypotheses that all answers should be randomly distributed and secondly that all answers should be the same between the robot and the human.
Is there a better more in-depth way of analysing this data? I have been scouring the literature but can't find anything.
What criteria are most important for you to trust a human? (you can answer multiple answers)
Dear all, i want to get the protocol for isolation of Lumpy Skin Disease Virus from ticks by Pathogen Free Egg؟؟؟؟؟؟؟؟
In my confusion matrix, I'm using one of the following two lines to change the font size of all the elements of a confusion matrix. But the following code changes font size includig title, tick labels and etc. How can I change the font size and color of the matrix elements by suppressing changes of other stuffs? Thanks in advance to help me.
% set(findobj(gca,'type','text'),'fontsize',20); % All font size
% set(findall(gcf,'-property','FontSize'),'FontSize',20); % All font size
I am working on detection of rickettsia in ticks, while I use a primer whose annealing temperature in the original paper 48, I always get non specific bands without appearance of the specific one
If I increase the annealing to 55 or 56 will this increase can get me the specific band ? for your information I dont have a positive control
In physics, the begin of the universe poses interpretation problems. This can be resolved by restricting the range of proper time to a subset of the range of events.
2 Physical reality
Physical reality appears to exist for about 13.8 billion years. Thus, after that starting instant the proper time clock ticks. Before that instant the creation of physical reality must have taken some action but that phase is not covered by the proper time range that observers can perceive. Any model of physical reality must incorporate this preparation phase and must deliver a proper explanation why the observable proper time range starts after the preparation phase finished. With other words the model of physical reality must belong to the category of the self-creating models.
3 Modeling physical reality
The preparation phase must generate a base model in which physical reality can evolve from a founding structure to a full-blown dynamic model that can be observed as the dynamic field that represents the universe, which exists in physical reality. That universe must be filled with all the discrete objects that can be observed in this field.
4 The Hilbert Book Model
The Hilbert Book Model is a self-creating model that owns a simple foundation from which a base model emerges that offers a huge structured read-only repository that archives all dynamic geometric data of all elementary particles that are embedded in a dynamic field. It uses quaternions as storage bins that contain a scalar time-stamp and a three-dimensional location. After sequencing the time-stamps, the archive tells the life stories of the elementary particles. The range of proper time values corresponds to the range of the archived time-stamps. Thus, this arrangement fits the requirements for the self-creating model.
In the preparation phase, for each elementary particle, a private stochastic process generates the hop landing locations of an ongoing hopping path that recurrently regenerates a coherent hop landing location swarm. The characteristic function of this process ensures that the same location density distribution describes this hop landing location swarm. After sequencing the time-stamps, the archived hopping path tells the life story of the elementary particle in the realm of its floating platform. That platform is implemented by a private quaternionic separable Hilbert space.
This life story corresponds with the ongoing embedding of the hop landing locations into the dynamic field that we call our universe, and that is implemented by a continuum eigenspace of a normal operator that resides in a background non-separable Hilbert space. All elementary particles embed their hop landings in this dynamic field.
The elementary particles act as elementary modules. Together they constitute all modules and modular systems that exist in our universe. Thus this dynamic field reflects the activity of all elementary particles. Each module can act as an observer. All observers must travel with a scanning proper time window. They can only receive information via the embedding field, and for them, that information was archived with a historical timestamp.
Thus, the model covers a larger number of activities than the observable range of proper time covers.
4.1 Two views
This is why the Hilbert Book Model supports a creator's view as well as an observer's view. The creator's view covers all activities. The perception of the modules is restricted to the observer's view.
I am using Qiagen blood and tissue kit for extracting of DNA from ticks I elute the DNA after incubation 1minute as manufacturers instructions
If I increase the elusion step to more than 1 minute to maximize the DNA yeild does this decrease purity of DNA?
I will collect skin biopsies from patients, but for practical reasons I will need to cryopreserve them for a few months up to 1 year before carrying out either primary cell isolation (fibroblast and keratinocytes), or full tickness skin culture. Anyone with relevant experience who could help me with references/suggestions?
I have used published primers to detect bebasia and theileria genus from ticks. I am getting bands in first PCR but not getting bands in nested PCR. I am using 5 ul product of first PCR for nested PCR.
I have carried out an experiment on cells from the same cell line, grown in two plates and exposed to different conditions (Control vs Treated)
When I collected the cells after n days of treatment, I carried out PCR targetting a specific gene, then normalised to a housekeeping gene, I carried out analysis using the DDCT method.
I now have the DCT values in a spreadsheet waiting for statistical analysis- however, I am not sure which test to use, or whether I should use DCT or DDCT values.
My DCT results seem to tick the boxes for a non-parametric test, but I am not sure if it should be paired or unpaired. My logic is that they could be paired, since they came from the same cell line, or that they could be unpaired since I am comparing Day n (control) vs Day n (treated).
Control= 12.59 11.19 12.31 10.89 11.08 11.32 10.97 8.48 10.25
Treated= 11.28 10.98 10.35 11.39 10.36 10.83 10.77 7.91 9.8
-1.31 -0.21 -1.97 0.49 -0.73 -0.49 -0.2 -0.57 -0.45
Please let me know if you would like more detail. I'm still something of a statistics beginner, so I'm not sure how much depth is required to be able to answer this question.
The aim of your project is similar to our goals.
Our PhD. student work with Tribeč virus. Currently, we have isolated a strain of TRBV. We were curious how does it replicate in different host cell lines. During 25 serial passages in three different cell lines (VeroE6, A549 and BHK21), we have observed the presence of 3 sizes of plaques. Now, we would like to isolate them and genetically characterize. I do not have experience with plaque isolation. If you do, please, could you describe how to do it?
We use 2% carboxymethylcellulose for plaque formation test.
We would also like to do the same in tick cell line, but I did not find any in cell culture collection.
With best regards,
Are you interested in Medical and Veterinary Entomology as well as Global Health?
An Entomology Summer School, entitled “Hands on’ Course on Arthropods of Medical and Veterinary Significance: A global perspective, from theory to practice”, will be held from the 26th till the 30th of August 2019 at the National Vet School of Toulouse (ENVT), France.
Please find the complete programme leaflet attached to this message.
The course will encompass topics such as arthropod-borne diseases, resistance in arthropod populations, control tools, principles of laboratory rearing and morphological identification of arthropods of medical and veterinary importance (i.e. mosquitoes, flies, sand flies, fleas, ticks, mites, etc.).
The course targets entomologists, postgraduate students in this field, , post-doctoral scientists ACVM and EVPC residents, pest control professionals and the like.
Applications to attend will be accepted until the 31st of March.
See you in Toulouse?!
I registered recently with account and probably by mistake I ticked one of the boxed which insert others profile to mine. So, How can I fix that.
My work is on detection of pathogen in ticks so I work on small amount of DNA tamplet copies. while I used Qiagen DNA extraction kit form blood and tissue I am still confused with choosing one of these kits especially as top taq mastermix kit use a thermostable DNA polymerase from a source other than the standard taq polymerase orginated from thermas aquaticus in the taq mastermix kit
both have the same extension rate and fidelity
Does it really matter the type of the thermostable polmerase in this situation ?
I received a tick in a folded over piece of scotch tape. I snipped most of the tape away from the tick. What solvent can I use to dissolve the scotch tape without compromising the quality of the tick DNA for downstream PCR? I tried quick dips in Heme D, xylene, and 95% EtoH but the tape is not dissolving.
I am unclear if antibody tests can be conclusive in a tick or even if it is possible to test. If so, what are the limitations once the ticks have been collected. Limits such as temporal limits, storage in ethanol, and shelf life.
I'm thinking of testing collect ticks/tissue in my lab (gasp)! I'm definitely a behavior/ field biologist but I thought I could start attempting to screen with established protocols..maybe...
I am giving you an example of my questionnaire below and intended objectives:
1) Do you take into account the education of entrepreneurs for approving a business credit application? (Nominal or Categrical)
2) If yes, which of the following education level is favoured by your bank to advance bank credit? (Categorical)
c) University Degree
d) other (Please Specify)
3) In financing SMEs how important do you think the education level of entrepreneurs in approving bank credit? ( likert scale)
d) quite important
e) very important
In my study, 35 credit managers have participated. I want to statistically test the association between categorical and ordinal variables? Let assume that 10 say they do not take into account the education of entrepreneurs for approving a business credit application. 25 said yes, and they ticked different level of education. People who said yes and have ticked different level of education, and have rated the education level differently on the likert scale (Some said little important, some said quite important). I want to statistically test the association between them (categorical variables and ordinal variable).
If log linear analysis is not appropriate, what is other way to statistically test such questionnaire.
Many Many thanks in Advance.
I am trying to find a new checklist of Iranian tick species and some information on ticks transmitting viral diseases, like CCHF. How many genera and species of ticks exist in Iran? Is there any complete references on these two topics?
Any suggestions and any related references would be greatly appreciated.