Science topic

Tick-Borne Diseases - Science topic

Bacterial, viral, or parasitic diseases transmitted to humans and animals by the bite of infected ticks. The families Ixodidae and Argasidae contain many bloodsucking species that are important pests of man and domestic birds and mammals and probably exceed all other arthropods in the number and variety of disease agents they transmit. Many of the tick-borne diseases are zoonotic.
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Theileriosis is a tick borne disease recorded in ruminants. Besides causing anemia it affects the respiratory tract thereby causing pulmonary oedema.
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Yes as a symptomatic treatment, and paying attention to not induce hypotension as secondary effect in weak animals. Buparvaquone remains the best etiologic treatment of theileriosis.
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Dear Collegues,
Are you interested in Medical and Veterinary Entomology as well as Global Health?
An Entomology Summer School, entitled “Hands on’ Course on Arthropods of Medical and Veterinary Significance: A global perspective, from theory to practice”, will be held from the 26th till the 30th of August 2019 at the National Vet School of Toulouse (ENVT), France.
Please find the complete programme leaflet attached to this message.
The course will encompass topics such as arthropod-borne diseases, resistance in arthropod populations, control tools, principles of laboratory rearing and morphological identification of arthropods of medical and veterinary importance (i.e. mosquitoes, flies, sand flies, fleas, ticks, mites, etc.).
The course targets entomologists, postgraduate students in this field, , post-doctoral scientists ACVM and EVPC residents, pest control professionals and the like.
Applications to attend will be accepted until the 31st of March.
See you in Toulouse?!
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thank you for interest.yes I do.
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That is a frequent question concerning Ixodes ricinus asked by public. And there are two groups of parasitologists answering - the ones who say "maybe" and the others who say "have some doubts". Is there anybody aware of studies solving this issue? Experimental evidence wanted!!!
Libor Mikeš
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As far as I know, there were no studies concerning the topic. I think that different substances might provoke different responses. In any case, this is a very interesting question.
Best wishes.
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There is an urgent need for Bartonella-specific antigens for use in gauging effector and memory T-cell responses. I am looking for a reputable source from anywhere.
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Thank you Monyer, for pointing me in a helpful direction. 
Best regards,
Keith
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In Lyme disease research, routinely vector tick(I. ricinus) is pressed and its hemolymph could be search for B. burgdorferi under dark field microscopy.
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Here is a paper showing that B. hermsii is persistently present in the salivary glands of its vector O. hermsii:
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To start of I am trying to isolate DNA from blood and ticks for the identification of the tick species and a parasite that is transmitted by the ticks, by running it through agarose gel, and a limited budget.
To be clear about this question, I get no DNA from Rhipicephalus decloratus ticks when using the Kapa Express Extract kit that can extract DNA from insects. I have followed the instructions to the letter. The only unclear thing in the instructions is that i should spin at high speed for 1 min.
Can anyone please help me by telling me perhaps at what speed this is(rpm or xg).
Alternatively if someone has experience with this kit and ticks please provide me with a few pointers or alterations to the protocol to get the maximum amount of DNA (dilution is a possibility afterwords) 
And please remember that this is before the PCR is performed (which does not work due to no DNA).
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You can spin at 10,000 rpm for 1 to 2 min.  Ensure to dilute the eluted sample as indicated in the protocol for successful PCR.  Since, there is no amplification, you need to see if DNA is present at first and then if it is good enough for PCR.  If DNA is present then the problem is with the quality of the isolated DNA.  So, try isolating from a blood [as a control] and with tick [shell removed].  After final elution, retain 5 ul and load 20 to 25 ul of the elute in the agarose gel and run to see if DNA is present.  If you see the DNA then go for PCR.  If PCR does not work, then ethanol precipitate the elute, dry and then dilute in sterile water.  
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I need a contacts!
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We have ticks from Moskow region  in sufficient quantities.We have a laboratory culture too, but in small quantities. In order to share them, we need them to propagate. It takes time. Galina Karganova
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I have started a Questionnaire Survey across few villages in the fringe zone of a Protected Area (PA) to gather information on prevalence of tick-borne zoonotic diseases. I would like to know the methods of determining the sample size for my study so that I can design a robust framework for the same. Why is 20% of the population regarded in some studies as an ideal sample size?
With regards
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Sample size will depend upon the expected prevalence proportion (p) that you want to estimate. use the formula n= [3.84 * p * (1-p)] / (l * l), where l is the allowable error in your estimate, taken usually as 10 to 20% of p. The value 3.84 corresponds to 95% confidence in your estimate. Replace it with square of 2.58, if you want 99% confidence. If sample is not a random sample, multiply by the design effect, taken usually between 1.5 and 2. If you expect some non-response from your study subjects, divide by the response rate proportion.
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As for ticks inhabiting forest formations I estimate the possibility of controlling them using biological agents as slim. Forest ticks are members of biocoenoses formed long ago and there is little chance of destroying the well developed balance of such biocoenoses by introducing new agents.
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Dear Researchers,
Is there any publication about this issue?
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Viruses are transmitted to the host just during the first minutes after tick attachment. This was demonstrated for tick-borne encephalitis virus and for Powassan virus. As far as I know there is no special tests for CCHF virus but I think that the rule is common for all tick-transmitted viruses.
Best regards.
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There has been extensive work published on tick-borne diseases in the Indian sub-continent, mostly from the Veterinary Science sector. But I am in need of a descriptive survey on the prevalence, status and distribution of tick species reported in North-eastern India for my PhD work. 
Thanks
Debabrata Phukon
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i had collected this ticks on the brown hare, i know that those parasites are rhipicephalus but please if you can confirm me the species.
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Ok now that I have a location I would not be surprised if it was  Rhipicephalus tricuspis.  That species seems fairly common on rabbits in the region but you should really try to get the key in "The Genus Rhipicephalus (Acari, Ixodidae) A Guide to the Brown Ticks of the World" by Walker et al.
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these ticks are collected from wild rabbits please anyone can confirm me their species.
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First two photographs  belongs to nymphal stage of Hyalomma sp ticks due to longirostrum , bifid  first coxa and sickle shaped ventral plates, Other 3 photographs belongs to Hemaphysalis sp ticks due o the presence of lateral prolongation in the third segment of pedipalp , spur in the first coxa and absence of ventral plates
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I am looking for a researcher working on Babesiosis to collaborate with, in order to detect IgG antibodies on human and bovine serum samples from Colombia. Any suggestion?
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La Unidad de Babesiosis del Centro Nacional de Parasitología del INIFAP en Cuernavaca, México realiza tales diagnósticos y mediciones rutinariamente. Sin embargo juzgo muy difícil poder trabajar muestras de sueros de bovinos de Colombia, por las restricciones zoosanitarias. Indudablemente los colegas de EMBRAPA en Brasil ó del INTA en Argentina, son reconocidos internacionalmente y pueden apoyarlos. Igualmente, me imagino el Dr. Benavides que estuvo en CORPOICA muchos años y ahora se encuentra en la Universidad La Salle, podría ser una ayuda más próxima. Ánimo.
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I am looking for experimental evidence (or convincing non-experimental evidence) that the number of fleas or ticks an individual animal harbors is related to that individual's risk of infection by a vector-borne pathogen.  Can anyone point me to some compelling literature?
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I believe the main issue is the level of infectivity. Too many ectoparasites may be present but not carrying the causative agent( Protozoa) while just one infected tick is enough to transmit a tick borne disrespectdisrespect
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Theileria camelensis, piroplasma spp., babesia spp. have been recovered from blood of dromedaries and Bactrian but they are non-pathogenic. Old world camels contrary to cattle and small stock seems not to be affected by serious tick-borne diseases such as East Coast Fever, Piroplasmosis, Anaplasmosis
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Firstly I'm not sure if we should expect tick paralysis in camels!
Secondly, only experimental infection of camels with already confirmed species (T. equi, B. cabali, T. mutans and T. annulata) can clear that whether camels are host of piroplasmids or just accidentally bitten by infected vectors. 
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Why does the alpha-Gal allergy affect some people and not others - is it caused by reaction to tick saliva/enzyme or is it a tick borne disease transmitted to humans or is there a genetic cause/immune system?
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The alpha-gal is a sugar that bounds to proteins, changing their allergenicity. It is more probable that the tick enzyme sensitizes the immune system in order to create a cross-reaction  sensitization to other similar alpha-gal epitopes, that produce the allergic reactions.
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Kyasanur Forest disease (KFD) is caused by Kyasanur Forest disease virus (KFDV), a member of the virus family Flaviviridae.
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Contact the Director, NIV, Pune. This Institute started . insixties as 'Vrus Research Centre to deal with KFD.
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I need update keys for differentiation of D. marginatus from D. niveus?
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The key for identification of all stages of Dermacentor ticks including D. marginatus and D. niveus has been given by Natalya Filippova in her book "Ixodid ticks of subfamily Amblyomminae" published in St.Petersburg in 1997 ("Nauka" Publishing House). This is the best key for these species but unfortunately it is only in Russian.
All the best,
Igor Uspensky.
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Does it mean that yellow ticks have only taken up plasma? Will such ticks moult successfully? I want to know the mechanism behind this phenomenon.
Please see below picture.
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This is an interesting question - we certainly see yellow engorged female R microplus in many cases. We also know that many ticks ingest huge numbers of leukocytes when they feed (see pic - green cells are neutrophils - courtesy Constantin Constantinoiu). We have never looked at the yellow ticks specifically, but I guess they have a higher proportion of leukocytes. Regards, Nick
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I want to buy a GPS device to be used for vector mapping in Sudan and low income countries.
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To me, depends on precision you require, if you do not precision of more than 10-30 meters between one point and another you can you your smart phone with a GPS application, otherwise if you need more than that you will need a professional GPS with more accuracy. From 2015 a new technology of GPS is coming, more accurate, so if its not urgent and so accurate, smart phone, if not, wait for 2015.
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I want new information on the diagnosis of Hyalomma marginatum from H. detritum. . Can anyone help me?
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Hi,
Hyalomma detritum is now called H. scupense. Are you adking how to differentiate between the two species?
All the best
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Is there any data available indicating tick infestation and reduced reproduction in domestic animals or in any animal species?
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The infestation of poultry with Argas persicus follows with reduction of egg production (Adene & Dipeolu, 1975, Bull. Animal Health Production in Africa, 23: 333-335). I don't know studies concerning reduction in animal reproduction after tick infestation, such studies mainly deal with loss of blood, emaciation and mortality. Try and see an interesting paper by Patterson et al., 2013, PLoS ONE, 8(2).
Best regards.
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I have DNA from babesia bovis and bigemina from clinical and non clinical cases.
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I want to study Is babesia spp. in Egypt have resistance to the used drugs thereby I want follow which method will be valid and give successful rates in identification of the resistance at all or not present
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Epi-Info I understand now has some mapping capabilities, but I haven't used it. It is free. There is of course ArcView, which is not free. Some other free ones are CrimeStat and SatScan, both excellent and easier to use than ARcView. There are probably others that are free.
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I am a Phd student and I want to work on a hard ticks survey and their tick born diseases in the Mazandaran provinces in Iran.
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Asim Shamim
Thanks for youe comment. Please give me Qijen kit method protocole.
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I am looking into a large number of samples of deer and wild rodents. The tick borne pathogens, inside each genus, are too similar to differentiate and cloning will increase the number of samples greatly. I have been using primers for Ehrlichia, Anaplasma, Babesia and Theileria species but these primers are not specific and they cross reacted with other strains. In turn, the sequencing of the nested PCR positive samples failed (may be due to coinfection with multiple pathogens which were picked up with the same primer). I am thinking of switching to RLB but I don't know how sensitive and specific this method is in comparison to the nested PCR, cloning and sequencing.
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I would consider the PCR-RLB approach too. More sensitive and specific compared with PCR alone, plus the advantage of screening a huge number of specimens for multiple pathogens at a go.