TUNEL - Science topic

Las grandes obras de infraestructura urbana suelen concitar tanto entusiasmo como malestar. A veces no necesitan pasar muchos años para que broten diversas evaluaciones críticas y es muy común que se llegue a considerar que hubiese sido preferible no haberlas construido (Flyvbjerg, 2018). El Sistema de Drenaje Profundo de la Ciudad de México, el me...

Secondary injuries from ischemia‒reperfusion in stroke, such as edema and hemorrhagic transformation, can significantly impact brain function. This study investigated the effects of intermittent theta burst stimulation (iTBS) on neurological function and cerebral blood flow in a mouse model of ischemia‒reperfusion injury. Laser speckle flowmetry wa...

This work reported the neuronal protection of low-dose lipopolysaccharide (LD-LPS) after spinal cord injury (SCI). SCI rat model was constructed, after adenovirus-mediated ALKBH5 vectors and shRNA transfection and LD-LPS pre-treatment. Hematoxylin and eosin, Nissl, TUNEL staining of spinal cord tissues were adopted to monitor pathological changes,...

Exercise training can exert favorable effects on the remodeling of neural structures, functional reconstruction, and the recovery of neurovascular units after cerebral ischemia. It has also been documented to activate the JNK/c-jun pathway, which is involved in diverse cellular processes, including growth regulation, transformation, and programmed...

En el presente trabajo se muestra un análisis técnico-económico del consumo energético térmico de un secador solar mixto tipo túnel para el procesamiento de carne de res. La energía es proporcionada por un sistema solar que consiste de un campo de colectores solares de placa plana interconectados entre sí y un tanque de almacenamiento térmico princ...

This study aimed to investigate the mechanism of ginsenoside Rg1 in Alzheimer's disease (AD) via miR-9-5p/SIRT1-mediated mitochondrial function. The cognitive function of AD mice was assessed by Morris water maze experiment. The histopathological changes in the CA1 region were observed by H&E staining. TUNEL staining combined with the neuronal mark...

Apoptosis is the primary pathological feature of neuronal injury caused by cerebral ischemia-reperfusion (I/R). The detailed molecular mediators are still being debated. This study aims to examine the effects of cerebral ischemia-reperfusion on apoptosis and NGF-Akt/Bad axis in rat hippocampus alone and in combination with NAC (N-Acetylcysteine). R...

The incidence of myocardial infarction (MI) has been increasing in recent years, and the cause of acute myocardial infarction is apoptosis due to insufficient coronary myocardial blood supply. PLAC8 is a critical gene in the disease process of MI through GEO database research and analysis of differentially expressed genes (DEGs). In this study, in...

This study aimed to investigate the beneficial effects of iron isomaltoside (IIM) on myocardial function and the associated mechanisms in rats with myocardial ischemia/reperfusion (I/R)-induced damage and hypoxia/reoxygenation (H/R)-induced H9C2 cells. Changes in cardiac pathology after myocardial infarction (MI) were analyzed with hematoxylin-eosi...

Spinal cord injury (SCI) has a high mortality and disability rate. Endoplasmic reticulum (ER) stress induces neuronal apoptosis and participates in the regulation of SCI. LncRNA ZFAS1 can participate in the regulation of SCI by influencing ER stress; however, its mechanism is worth further exploring. The molecular mechanism of lncRNA ZFAS1 regulati...

Activating transcription factor 3 (ATF3) may function as a regulator of various diseases; however, its role in spinal cord injury (SCI) remains unknown. We designed a current work to evaluate the potentials of the ATF3/forkhead box protein A2 (FOXA2) axis in SCI. GSE45006 chip was analyzed, and a volcano plot and heatmap were drawn. Gene Ontology a...

Hyperglycemia is a key factor in diabetic retinopathy which leads to blindness. O-linked-N-acetylglucosamine (O-GlcNAc) modification changes are linked to various diseases, including diabetic retinopathy. This research aims to study the roles of Txnip and Trx in influencing O-GlcNAc in photoreceptor cells during diabetic retinopathy. A diabetic mou...

Gold nanoparticles (AuNPs) possess unique properties for various biological and intraocular applications. In retina research, AuNPs hold promise for therapeutic intervention, drug delivery, and photoreceptor replacement, but their potential toxicity is an area of concern. This study assesses the toxicity of AuNPs formulations suitable for photorece...

Background Peripheral nerve injury (PNI) is currently an intractable disease for clinical therapeutics. Schwann cells (SCs) play pivotal roles in peripheral nerve regeneration. Naringin, a natural flavonoid, has potent beneficial effects on peripheral neuropathy. Researchers have rarely reported whether naringin attenuates SC apoptosis and the rele...

Background Diabetes mellitus is a disordered syndrome which has destructive effects on cells. The aim of this study is to reduce the side effects caused by glucose using treating cells with hydroalcoholic extract of Prosopis farcta in electrospun 3D culture with poly lactic acid (PLA). Materials and methods The extract was prepared by maceration m...

El escarabajo barrenador del tallo (Xyleborus ferrugineus) representa una grave amenaza para el cultivo de cacao (Theobroma cacao) en México, especialmente en Tabasco y Chiapas, regiones que concentran más del 90% de la producción nacional. Este insecto, de 2-3.3 mm de longitud (hembras), excava túneles bajo la corteza donde cultiva hongos simbióti...

Background Air pollution and obesity are crucial risk factors for cardiovascular disease (CVD), with epidemiological evidence indicating that air pollution exacerbates obesity-induced cardiac damage. Treatment with adipose-derived stem cells (ADSCs) attenuates cardiac damage by releasing paracrine factors. However, the effects of ADSCs on air pollu...

Neonatal hypoxic-ischemic encephalopathy, a severe consequence of birth asphyxia, remains a leading cause of newborn mortality and permanent neurological disabilities worldwide. The limited therapeutic options and incomplete understanding of its pathological mechanisms present significant challenges in clinical management. This study aimed to inves...

Background NOD-like receptor thermal protein domain associated protein 3 (NLRP3)-mediated pyroptosis of cardiomyocytes is a key contributor to the progression of myocardial infarction (MI). This study aimed to investigate whether exosomes derived from human induced pluripotent stem cell-derived mesenchymal stem cells (iPSC-MSC-EXOs) could protect a...

Background Acute pancreatitis (AP), characterized by pancreatic autodigestion and systemic inflammation, remains a life-threatening condition with a high mortality rate in severe cases. Cholinergic signaling through muscarinic receptors (mAChRs) regulates pancreatic exocrine secretion, yet the role of chronic cholinergic hyperactivation in AP progr...

Background The strong genotype dependence of embryogenic callus (EC) induction limits the high-frequency regeneration of Korean pine (Pinus koraiensis) via somatic embryogenesis (SE). Methods We examined the morphological and histocytological features of various callus types using external morphology, microstructure, and ultrastructure analyses. T...

A aerodinâmica é uma área que cada vez mais ganha visibilidade no mercado. Alinhado com o desejo de análise do Gurney Flap, inserido no bordo de fuga de um aerofólio, optou-se pela análise experimental em túnel de vento subsônico da Univap. Após um levantamento teórico, foi criado um modelo 3D utilizando um software de CAD. Foram elaboradas duas ve...

Clinical studies have reported that anemic patients with lower back pain exhibit significantly more severe lumbar intervertebral disc (IVD) degeneration compared to non-anemic individuals. Given that iron deficiency (ID) is the leading cause of anemia, we hypothesized that ID may disrupt IVD metabolism—particularly extracellular matrix synthesis—th...

Purpose The purpose of this study was to evaluate the efficacy and safety of accelerated scleral collagen cross-linking (SXL) with supplemental oxygen. Methods The rabbit scleral tissues were randomly divided into seven different SXL protocols, including traditional (T)-SXL (3 mW/cm² for 30 minutes, 3*30) and accelerated (A)-SXL (30 mW/cm² for 3,...

Başka bir dünyanın mümkün olup olmadığı sorusu, içinde bulunulan çağın ekolojik, toplumsal ve teknolojik krizleri karşısında giderek daha belirleyici hale gelir. İklim değişikliği, biyolojik çeşitliliğin azalması ve ekosistemlerin çöküşü, insanın doğayla kurduğu tahakküm ilişkisini sorgulamayı zorunlu kılar. Bu bağlamda, Donna Haraway’in Staying wi...

Oxidative stress-induced renal tubular epithelial cell apoptosis or dysfunction promotes the progression of acute kidney injury and chronic kidney disease. Neng-Jing-Huo (NJH), an essential oil mixture of Gaultheria procumbens, Zingiber officinale, Bulnesia sarmientoi, Artemisia vulgaris, and Styrax benzoin, exhibits antioxidant potential. However,...

Background Dilated cardiomyopathy (DCM) is a severe and irreversible heart disease characterized by dilated ventricles and decreased myocardial function. DCM has a poor prognosis and a very low survival rate, with a 5-year mortality rate ranging from 15 to 50%, and is an important cause of sudden cardiac death and heart failure. Genetic factors pla...

Background This study aimed to evaluate the cytotoxicity and genotoxicity of AH Plus, Well-Root ST, and AH Plus Bioceramic Sealer on osteoblast cells. Methods To prepare AH Plus, equal amounts of paste A and paste B were mixed following the manufacturer's instructions. The AH Plus BCS and Well Root ST syringes were fitted with appropriate cannulas...

The intra- and intertumoral heterogeneity of gliomas present major challenges to effective chemotherapy. This study explored the combined effects of PRMT5 and MAT2A inhibitors on glioma progression. The expression of drug targets was determined in cell models using western blotting and immunofluorescence assay. CCK-8, colony-formation, EdU fluoresc...

Nuclear - contaminated water, a concerning byproduct of nuclear mishaps and industrial operations, contains radioactive elements. These substances pose substantial threats to both human health and the environment. Since the release of processed water contaminated with nuclear substances into the Pacific Ocean in 2023, global concerns have been moun...

Background: Lactylation, a novel lactate-derived posttranslational modification, has been demonstrated to be linked with brain function. The present research is intended to explore the role of β-arrestin1 (ARRB1) lactylation post-subarachnoid hemorrhage (SAH). Methods: SAH models were established in mice via intravascular puncture and in primary ne...

PURPOSE: The carcinogenic effects of Ultraviolet B (UVB) radiation have been demonstrated in vitro and in animal models. However, these models have limitations, as they do not fully replicate human cellular organization and responses. This creates a significant gap in our understanding, particularly regarding the development of therapeutic mechanis...

EFECTO TUNEL

Sinusoidal obstruction syndrome (SOS) is a fatal liver condition resulting from sinusoidal endothelial cell injury and hepatocyte death, following liver or hematopoietic stem cell transplantation as well as chemotherapy. We showed evidence of platelet displacement and aggregation within the space of Disse in SOS. However, the relationship between p...

The culture of Litopenaeus vannamei, a key species in global aquaculture, has expanded significantly, especially in regions utilizing saline-alkaline water resources. However, the impact of high pH and alkalinity on shrimp health remains a critical concern. This study investigates the molecular effects of combined high pH and alkalinity stress on s...

Background/Aim: Conventional therapies for triple-negative breast cancer (TNBC) usually exhibit low efficacy. This study aimed to demonstrate the feasibility and antitumor efficacy of intratumoral (IT) injection of allogeneic natural killer (NK) cells with or without chemotherapy in the TNBC preclinical model. Materials and Methods: The TNBC precli...

Gastrodin (Gas) is a key active ingredients of Gastrodia elata Bl., with applications in treating cardiovascular and neurodegenerative conditions. However, the impact of Gas on neuronal damage caused by cerebral ischemia/reperfusion remains uncertain. A cell model of oxygen-glucose deprivation/reoxygenation (OGD/R) was established and the viability...

Background Spinal cord injury (SCI) is a neurological disorder characterized by severe and often irreversible damage to the spinal cord, for which no effective treatments currently exist. Ubiquitination, a reversible post-translational modification, plays a critical role in regulating protein degradation and stabilization. Tripartite motif-containi...

Background Coronary heart disease (CHD) ranks as the primary cause of global morbidity and mortality. Despite of the progress in exploring risk factors and developing medications, considerable residual risk persists. In recent years, epigenetic regulation has emerged as a critical regulatory mechanism across various diseases. This study aimed to in...

Aim To explore the benefits of Morinda officinalis oligosaccharides (MOO) on ischemia-reperfusion (I/R) injury and the possible mechanisms involved. Methods Myocardial I/R injury were induced by left anterior descending branch ligation. MOO pretreatment was given orally 2 weeks prior to ischemic treatment. Echocardiograms, biochemical parameters,...

Acute lung injury (ALI) induced by sepsis is a serious life-threatening disease, one of its characteristics is the polarization of macrophages. Circ_0008285 has been found to be associated with various diseases. In this study, we detected the regulatory role and mechanism of circ_0008285 in sepsis-induced ALI. RAW264.7 cells treated with LPS and C5...

Background Due to high heterogeneity, diagnosing MDS can be challenging. Consequently, investigating its pathogenesis and progression mechanisms, and seeking novel targets for diagnosis and treatment, are critical issues that require urgent attention. This study aimed to investigate whether LRRFIP1 might contribute to MDS pathogenesis by modulating...

The incidence of stress‐induced gastritis is gradually increasing. Vitamin E (VE) is widely used in inflammatory diseases due to its efficient antioxidant and anti‐inflammatory effects. Here, we investigated the protective role of VE on stress‐induced gastritis and its potential mechanisms. Mice were subjected to high‐intensity stress caused by the...

Amaç: Karpal Tünel Sendromu (KTS), median sinirin el bileğindeki karpal tünel içerisinden geçerken sıkışması sonucu ortaya çıkan yaygın bir periferik nöropatidir. Semptomlar genellikle el ve bilekte uyuşma, karıncalanma, ağrı ve güçsüzlük şeklinde kendini gösterir. Bu sendrom, özellikle tekrarlayan el hareketlerinin sık olduğu meslek gruplarında ve...

Ethnopharmacological Relevance Wu-Hua-Yan-Xiao (WHYX) is an innovative volatile oil formulation derived from the traditional Yinqiao-Mabo decoction, developed for the treatment of pediatric acute pharyngitis. Materials and Methods Network pharmacology was utilized to identify active components and potential therapeutic targets of WHYX in acute pha...

Background Pancreatic cancer is one of the most lethal malignancies characterized by a complex tumor microenvironment (TME) and highly heterogeneous nature, making it resistant to radiotherapy. This study aims to evaluate the radiosensitizing effect of homocamptothecin derivative TOP-0618 on pancreatic cancer. Methods Clonogenic assays and cell vi...

Aim: Chronic atrophic gastritis (CAG), a precancerous lesion of gastric cancer. Current therapies face challenges including drug resistance and incomplete efficacy. Tanshinones exhibit anti-inflammatory and anti-tumor properties, yet their role in CAG remains unexplored. The present study aimed to explore the therapeutic effect and underlying mecha...

or in combination significantly reduced the percent of infarcted brain volume and TUNEL positivity suggesting that these agents may confer possible benefit in treatment of infants with hypoxic-ischemic encephalopathy. Aim In this study cerebral function monitor (CFM) findings were recorded and evaluated from the neonates associated with acute bilir...

Purpose The aim of this study was to explore how sulforaphane (SFN), a well-known nuclear factor erythroid 2-related factor 2 (Nrf2) pathway activator, regulates hepatic autophagy and apoptosis by modulating the polarization of Kupffer cells via the Nrf2/HO-1 pathway in a hemorrhagic shock/resuscitation (HS/R) model in mice. Methods Male c57/BL6 m...

Background The salivary glands of female ticks rapidly degenerate after feeding via programmed cell death mediated by an ecdysteroid receptor (ECR). The degeneration includes both apoptosis and autophagy. The process of degeneration can also be regulated by microRNAs (miRNAs), but the underlying mechanism of miRNA involvement in salivary gland dege...

Gastric cancer (GC), known for its aggressive growth and metastasis, remains a leading cause of cancer-related mortality. Although Timosaponin B-II (TB-II) from Anemarrhena asphodeloides has shown anticancer potential, its underlying mechanisms in GC are not yet fully understood. Our study investigates and compares the expression patterns of Kruppe...

Introduction Hydrogen gas has demonstrated significant antioxidant and anti-inflammatory properties, suggesting potential therapeutic benefits in TBI. Methods We subjected to controlled cortical impact in mice to construct TBI model. They received an intraperitoneal injection of MCC950, a selective NLRP3 inhibitor, at 10 mg/kg 30 min before TBI. I...

Background Hyperoxia plays a key role in the development of bronchopulmonary dysplasia (BPD), a chronic lung disease of preterm infants. This study aimed to investigate the role of NLRP3/caspase-1/gasdermin D (GSDMD)-mediated pyroptosis in hyperoxia-induced lung injury in neonatal mice and to evaluate the potential protective effects of the caspase...

Background In the present study, the synergistic effects and mechanism of recombinant viral vector-mediated co-expression plasmids stat1 and stat3-siRNA on glioma were investigated in vivo and in vitro. Methods Co-expression plasmids for stat1/stat3-siRNA were constructed and packaged into lentivirus and adenovirus for cell and animal experiments....

The presence of two ursids, Ursus deningeri hercynicus Rode, 1935 and Ursus arctos taubachensis Rode, 1935, was documented from the Tunel Wielki Cave. Of them, Ursus deningeri hercynicus is represented by almost all skeletal elements, with the predominance of isolated teeth, metapodials and phalanges. The analysis performed shows that the remains o...

Cutaneous melanoma is a malignant tumor with a high mortality rate. Ubiquitin-specific protease 41 (USP41) has recently been reported to be overexpressed in various malignancies. However, its role in melanoma remains unclear. Gene Expression Profiling Interactive Analysis (GEPIA) was used to perform pan-cancer analysis using data from the the Cance...

Uremic cardiomyopathy (UCM) is a leading cause of death in patients with chronic kidney disease. This study aimed to explore the role and underlying mechanisms of neutrophil extracellular traps (NETs) in UCM. A UCM mouse model was constructed using 5/6 nephrectomy and treated with the NET inhibitor DNaseI. Cardiac fibroblasts were incubated with 20...

Purpose The role of Long noncoding RNA OIP5-AS1 in myocardial ischemia/reperfusion (I/R) injury-induced apoptosis remains to be fully elucidated. The present study was conducted with the objective of investigating the function of OIP5-AS1 in myocardial I/R injury and exploring its potential mechanisms. Methods In order to simulate the conditions o...

Background/Objectives: Increasing evidence reveals the likely peripheral etiology of Parkinson’s disease; however, the mechanistic insight into α-Synuclein aggregation in the periphery remains unclear. This study aimed to explore the effect of abnormal expression of renalase on dopamine metabolism, toxic DOPAL generation, and subsequently, α-Synucl...

Introduction Proper placentation is essential for fetal growth and development in mammals. Nodal signaling is essential to ensure proper embryo development and requires Cripto-1 as a co-receptor. Both factors have been shown to be expressed in the maternal decidua and developing placenta. Notably, a maternal loss of either Nodal or Cripto-1 leads t...

Objective: Radioiodine (RAI) therapy is widely used for thyroid ablation, but I-131 accumulation in non-thyroid tissues may cause adverse effects. Vitamin D, known for its anti-apoptotic and immunomodulatory roles, may help protect against such damage. This study investigates the potential protective effects of vitamin D on RAI-induced liver injury...

BACKGROUND Spinal cord injury (SCI) is a severe and permanent trauma that often leads to significant motor, sensory, and autonomic dysfunction. Neuronal apoptosis is a major pathomechanism underlying secondary injury in SCI. Long non-coding RNAs (lncRNAs) have emerged as key regulators of gene expression and cellular processes, including apoptosis....

Ventilator-induced diaphragm dysfunction (VIDD) is one of the main causes of weaning from mechanical ventilation (MV). The forkhead box O3 (Foxo3) has been identified as being involved in regulating the contractile function of skeletal muscle. This study aimed to figure out the regulatory role and mechanism of Foxo3 on VIDD. The mouse myoblast C2C1...

Sperm preparation is a critical step in assisted reproduction, aiming to isolate spermatozoa with optimal characteristics and high fertilizing potential. Traditional sperm selection methods involve centrifugation, which may cause sperm damage. Microfluidic sperm sorting (MSS) offers an alternative approach, mimicking the female reproductive tract e...

Chondrocyte necroptosis contributes to the pathogenesis of osteoarthritis (OA). Pulsed electromagnetic field (PEMF) is a potentially useful treatment for OA. Here, magnetic nanoparticles and PEMF generate magneto‐mechanical forces for regulating signaling pathways, but their effectiveness remains unclear. This study investigated whether magnetic na...

Myo/Nog cells play a pivotal role in ocular development and demonstrate a rapid response to stress and injury. This study investigates their behavior and distribution in a murine model of retinitis pigmentosa, specifically in C3H/HeJ mice, which exhibit photoreceptor degeneration due to a homozygous mutation in the Pde6brd1 gene. Retinal samples fr...

Objective Osteoarthritis (OA), a high‐prevalence degenerative cartilage disease, urgently requires novel therapeutic strategies. M2 macrophage‐derived exosomes (M2‐Exo) demonstrate therapeutic potential for OA, though their regulatory mechanisms in chondrocyte‐macrophage (Mφ) interactions remain to be elucidated. To investigate the regulatory effec...

Long noncoding RNA (lncRNA) MEG3 has been considered as a novel target for alleviating the brain tissue damage during cerebral ischemia-reperfusion injury (CIRI). Numerous studies have reported that pyroptosis is involved in the pathogenesis of CIRI. This study focused on whether MEG3 modulates CIRI via pyroptosis and its underlying mechanism. The...

Introduction Orthosiphon aristatus (Blume) Miq. (OA) is widely used in folk medicine to treat kidney stones (KS). Its total flavonoids (OATF) are the primary active constituents responsible for its therapeutic effects. However, the exact mechanism of action (MOA) remains unclear. This study aimed to investigate the pharmacological activity of OATF...

Background Premature ovarian insufficiency (POI) poses a significant threat to female reproductive health and currently lacks effective interventions. Recent studies highlight the promising potential of human pluripotent stem cell-derived mesenchymal stem cells (hPSC-MSC) in regenerative medicine. However, research on hPSC-MSC-based treatments for...

Micronuclei serve as a biomarker of cancer cell due to genetic mutations and an indicator of deoxyribonucleic acid (DNA) damage. The aim of this study to examine the effect of hexadecanoic acid in Musa paradisiaca (MP) as anticancer by reducing the frequency of micronuclei and inducing apoptosis.. Twenty male rats were divided into five groups. Neg...

To investigate whether Liraglutide had a neuroprotective after cardiac arrest and return of spontaneous circulation (CA/ROSC) and explore its potential mechanisms. Adopting an 8-min asphyxial cardiac arrest model. Evaluate the neurological deficit score (NDS), observe pathological changes in hippocampal tissue with HE staining, and measure the expr...

Mutations in the pathogenic gene CDH23 are known to cause Usher syndrome, affecting both auditory and visual functions. Our previous results provided valuable insights into the mechanisms underlying congenital hearing loss associated with CDH23 mutations. However, the molecular mechanisms and signaling pathways that influence vision remain largely...

Long non‐coding RNAs (lncRNAs) play significant roles in neurological diseases, including epilepsy. Our previous study identified lncRNA‐GPHN as specifically downregulated in a rat model of status epilepticus (SE). Investigate the role and regulatory mechanism of lncRNA‐GPHN in the pathogenesis of epilepsy. SE rat and in vitro cell models were used...