Questions related to Surface Functionalization
As mentioned in the figure, we want to separate oil from water or water from oil through a graphene oxide sponge based on porosity/surface functionality.
The pore size of the graphene oxide sponge is so small that oil is not allowed to pass through it and only water will pass when the surface functionality of the graphene oxide sponge is oxygenated. Same way, water is not allowed to pass through it and only oil will pass when the surface functionality of the graphene oxide sponge is fluoridated.
This is my approach for oil and water separation, I don't know whether is this correct or not?
I would like to functionalize an ITO (indium tin oxide) substrate with a SAM (self-assembled monolayer), let's say with MPA (3-mercaptopropionic acid). The first step is obviously to properly clean the ITO in basic medium to activate the surface (then rinse with water and isopropanol). Then, the ITO is simply immersed in an aqueous MPA solution over night. 
This procedure seems very straightforward, but there are different protocols of varying complexity (different solvent, addition of acid, different concentration, ...). After following the protocol, I have no way of knowing whether the surface functionalization was successful or not. What kind if measurement can I perform to validate my approach? Ideally, I would like to come up with a number such as "The surface coverage of MPA is X mol/cm2." or "X % of the surface is covered with MPA." Is there a method to quantitatively describe my result?
 Lee et al. Langmuir 2003, 19, 10, 4338-4343
I've managed to functionalise my multiwalled carbon nanotubes using H2SO4:HNO3 (3:1) and have proceeded to the washing step via centrifugation and decanting. However, I am still unable to attain a pH of 6 to 7 as recommended even after 8 washes with ultrapure water. I saw some mentions regarding using a strong base like NaOH but I was wondering if it would affect my already functionalised MWCNTs. Aside from that, I couldn't find the explanation behind this step. Does anyone know how to increase the pH of my functionalised MWCNTs and the importance of that step?
How to linearize any of these surface functions (separately) near the origin?
I have attached the statement of the question, both as a screenshot, and as well as a PDF, for your perusal. Thank you.
I am trying to etch surface of glass beads through NaOH for creating hydrophobic surface. Although the material is getting etched, it's not hydrophobic or positively charged. Also, the NaOH based etching method is not much predictable either. Are there any methods for creating hydrophobicity and a positive charge condition on glass beads?
I have synthesized carbon dots that exhibit fluorescent properties, that are further coated with PEG through EDC. I want to separate them through gel electrophoresis based on the number of PEG attachment. Is the gel electrophoresis for Carbondots any different than gel electrophoresis for nucleic acids ?
I would like to know if there is any software that can be used to generate molecular surfaces functionalized/decorated with other molecular groups. For example, I want to start with a given XYZ/MOL2 file for a pristine structure (i.e. a carbon nanotube) and them, generate several other structures with other fragment (i.e. organic group -OH) attached randomly to its surface.
Hi there, I am doing functionalization of a citrate based polymer with EDEA using EDC Chemistry with NHS and MES. Can someone help me find a protocol for using EDC on a gel scaffolds. Most of the protocol I can find is by dissolving their protein or nanoparticle in MES buffer before adding the EDC and NHS. My sample is a gel and not in particle or in powder form and I am having trouble on how much and of what concentration of these reagents can I use to soak my gel and later on conjugating my amino. Hope someone who’s doing related research can help me with this one. Thanks
We are trying to use surface functioned microbead for protein conjugation. One major way is to use NHS functioned-bead. The manufactor also recommend the epoxy functioned bead. Both functionalisation can directly immobolise protein covalently. (aren't they?) Which conjugation reaction is more efficient?
I am using chemical surface functionalization of PDMS and Polyimide (PI) to achieve a irreversible bond. This method works really great and I have achieved irreversible bonding but I am not sure if this chemical process will cause any change in mechanical properties of PDMS?
In this process I have plasma treated PDMS and PI for 1 minutes and then immersed in 1% (v/v) solution of MPTMS in methanol and GPTMS in methanol for 1 h, respectively.
I have a spincoated thin film of P(VDF-TrFE). I'd like to attach a thicker, transparent film as a backing layer that would allow me to pull off the PVDF film without it crinkling. I have access to a variety of cleanroom tools and extensive experience in chemistry and surface functionalization.
What are some good candidate backing layers that will tolerate temperatures > 120 C? If functionalization or e-beam evaporation is needed, I prefer to do this to the backing layer, not to the PVDF. I'm currently looking at ways to functionalize PDMS, PET, and PI.
I need to functionalize a glass surface with DNA (my method of functionalization is crosslinking amine terminated DNA to aminosilanized glass with gluteraldehyde). I then need to bond that functionalized slide to PDMS.
Im afraid O2 plasma will destroy my DNA probes. Any papers on this?
Im going to try the following but i was wondering if anyone has any input on how well any of these alternate methods work/methods to enhance these:
-Glass + PDMS + weight ==> oven 80C (would a hot plate work?)
-Piranha treat PDMS
-Plasma treat just PDMS
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696384/ this is kind of intense, is there a way to do this without the UV?
What are the main differences between Graphene Oxide and Expanded graphite, in terms of surface functionalities, conductivity, d-spacing and other features? Is the term expanded graphite synonymous to exfoliated graphite? Kindly help.
- Is there a simple protocol for short-term coating or fixation (just some hours) of surfaces with silver nanoparticles (Heating? Irradiation ? pH ?) ? I wonder if there is a way that could work the same for many types of surfaces (metallic alloys, polycarbonates...), also I should not have any other organic or major inorganic residue after the coating.
- My goal is to obtain an AgNP coated surface for short biological experiments. The best is that I can use pre-existing nanoparticles radher than a protocol involving on-surface synthesis.
Thank you for your valuable advice.
i did titration to determine functional groups on the surface of activated carbon, when the titration was done on Na2HC03 and NaOH by HCl, a big volume was necessary to reach equilibrium, and after doing calculations, final quantity of matter was greater than the quantity of matter calculated at the beginning. It seems ambiguous.
Formites, any disease transmitting surfaces, may be coated to prevent cross contamination of preventable communicable diseases. The effort is to find products that do not alter the surface functionality and to maximize the time between initial application and refresh coatings.
Nanocellulose. PPE (Personal Protective Equipment). Lignin. Face Masks. Surface Functionalization. COVID-19. Protection
I am trying to functionalize PE films by UV treatment and using 5 % benzophenone as a photoinitiator. But I am not able to receive any bond shift even after a considerable exposure time. What could be the problem?
I have successfully synthesized lanthanide up-conversion nanoparticle using hydrothermal method, they are hydrophilic in nature with positive surface charge as confirmed through DLS.
I want to make surface charge negative.
I know there are lot of articles about surface functionalization, but most of them are related to switching from hydrophobic to hydrophillic.
can anyone please share the relevant information about it?
I'm looking for a method to attach APBA to a SU-8 surface. Until now, I'm not very successful in the process.
The SU-8 is a 1um film deposited over a surface. I'm trying to immerse the substrate in 80mM of APBA in ethanol.
Can you give me an advice?
PS: Chemical molecules and suppose reaction
In recent years Graphene quantum dots with very high Luminescence (Quantum yield) are reported. These QDs are generally dispersed in DI water (some time organic solvents as well). PL of GQDs in 400-500 nm (absorption 300-400 nm ) is credited to Surface functional groups (-OH,-COOH,-NH2).
But is it even possible to retain these optical properties if we try to obtain there GQDs in powder form or if we want to coat them on some substrate?
If yes then how to do it?
I am using the Boehm Titration for quantifying the surface functionalities of my materials. In the procedure of Boehm Titration, it says that, NaOH neutralizes carboxyl, phenolic and lactonic; Na2CO3 neutralizes carboxyl and lactonic; and NaHCO3 neutralizes only carboxyl groups. Does anyone can please explain why the NaHCO3 neutralizes only carboxyl group? similarly, why Na2CO3 neutralizes carboxyl and lactonic groups? and so on....
we want to know the relationship between the type of functional groups on a particle surface and the magnitude and type (+ or -) of zeta potential. For example when carboxyl groups are on the surface versus the amin functional groups.
As far as I know the PEG layer (or other functional group) can be formed in two ways on the surface of nanoparticles: mushroom or brush. Does this formation depend on whether the nanoparticle surface be flat or curved? in the other word, is the any relation between configuration of PEG layer and the shape of nanoparticle?
what could be the best way to characterize basalt fiber surface. I have coated Basalt fiber and from FTIR i did not get much information? Is there any other technique with more sensitivity for detection of surface functional groups?
Hi. I am using EDC-NHS chemistry to modify PLGA surfaces using proteins. Is there a certain reaction time for the amine reactive NHS ester after which it becomes inactive (or less reactive) and does not aid in further protein binding to the surface?
How does the hydrophilicity/hydrophobicity of a support (like carbons) affect the particle size of the metal particle ?
Does the surface functionalities on the carbon surface any effect on the size of the particle formed?
Good afternoon! I am currently working with PVDF membranes (pristine PVDF and PVDF surface functionalized with OH) (These membranes are not electrospun). I have attempted to tissue culture fibroblast cells onto the membranes to test for their adherence and have used the standard DMEM media (3T3 media) during this procedure. The media was changed every two days for 10 days, however it was noticed that the media would turn purple everytime (upon measuring its pH, the media has become more basic). The same observation has been noted even immediately post-sterilization when the membranes were soaked in the media for 30 minutes prior to starting the subculture process. Why has this occurred?
I'm working on a small project where I'm trying to covalently bond nanomaterial to polyethylene. For reasons to long to describe, there are -NH2 surface functionalities on the nanomaterial that I would like to use to bond to the polymer chain (polyethylene) and I've come to a bit of a standstill when trying to find a way to do so. Any ideas?
Hi, I want to probe single particle rotation at water-glass interface. Any suggestions on surface functionalization techniques on glass so the particles are still rotating in-plane but somehow stuck to the glass?
We are looking for a procedure to surface treat commercially available Er2O3 nanoparticles that do not have any surface coating to start with. The aim of the treatment is to cap Er2O3 nanoparticles with ligand molecules of short moiety spacing like MPA or MHA. There has been a large body of literature on how such surface functionality should be conducted for semiconducting nanoparticles like CdSe, ZnS and ZnO etc., but we haven’t been able to find a single paper on surface functionality of Er2O3 nanoparticles.
Can anyone tell if a procedure starting with dissolving Er2O3 nanoparticles in toluene, followed by adding MPA or MHA in the solvent, could lead to the passivation of Er2O3 nanoparticle surfaces by the thiol group (SH) of ω-mercaptocarboxylic acids, whereas the carboxyl group (COOH) of MPA or MHA may bind to the metal ion at a metal oxide surface? Thanks a lot for your attention.
We are optimizing the assay parameters for our TSH immunoassay. We had been using a 7.4 pH PBS buffer for antibody coating so far, but now want to evaluate if changing the pH of the coating buffer would enhance the binding of the TSH capture monoclonal antibody to the surface.
The surface is PMMA surface, modified with PEI to give amine-PMMA, further modified to give Glutaraldehyde as functional group on the surface.
Similarly, would changing the buffer for blocking buffer (currently using 1% BSA in PBS), improve binding?
Hi everyone, I'm trying to do epifluorescence imaging of a FITC-SH functionalized gold surface. The area is flat and of ca 200 um x 200 um. Currently I have been able to see only a dim light coming from this surface, barely distinguishable from the control (not functionlized gold) area. In addition, I am using a very long exposure time (20s) and high gain (x10) with a 20x-air objective, which seems a bit strange to me. Things didn't go much better by using a confocal microscope.
Could it be some quenching effect of the gold surface? Can the imaging in dry condition somehow affect the flourescence of the FITC? Thank you in advance for the help.
I want to use a Sonoplot Proto to functionalize a sensing area. For low viscosity liquids (1-2 cP) the humidity must be very high for it to work. I want to try coating with oxygen plasma to improve hydrophilicity. Does anyone have other suggestions ?
In polyelectrolyte (PE) assembly, PE solutions are often made at 1 mg/ml concentration however, solvent used varies. Some papers mention 0.5M NaCl solution pH 4.5, sodium acetate buffer pH 4.5, water, phosphate buffer saline pH 7.4 etc. pH should be in the range at which given polyelectrolyte remains in charged state. How one should decide which solvent/buffer viz. 0.5M NaCl or PBS or water (pH adjusted as per requirement) is ideal for his/her polyelectrolyte assembly experiment?
I have a short question to ask if it is possible to surface functionalise the aluminum micron size particle(which has a thin layer of Aluminum oxide on top of it) with APTES?
Thank you so much and I am looking forward to your kindly reply soon.
I am trying to functionalize silicon substrates with DNA. The idea is to use APTES to add amino groups on the native oxide layer and via a bifunctional linker, sulfo-SMCC, to link them to thiolated DNA.
I saw 2 publications doing so but with TEA buffer, don't understand why.
Hi dear all,
Recently, I am doing research using activated carbon (AC) cathode to reduce O2 for H2O2 generation. However, I found after saturated by reactive blue 19 (a dye), the performance of AC didn't decrease obviously, which confused me.
Therefore, I was wondering is it because the active sites for O2 reduction and adsorption are different?
Generally, the active sites include defects, surface functional groups, heteratom. Can I understand the difference from this aspects?
Thanks in advance!
I'm working on an electrochemical biosensor integrated into a microfluidic system. The working and counter electrodes are gold, and the reference electrode is Ag/AgCl.
I'm planning to either immobilize thiolized nucleic acids on the bare gold WE surface, or use 11-mercaptoundecanoic acid SAM method to functionalize the WE surface for EDC-NHS coupling to proteins.
Due to the way the microfluidic device is fabricated, I may need to immobilize the biorecognition probes within closed channels (post-bonding). I also want to functionalize only the WE, while keeping the CE/RE uncoated.
Can anyone guide me on how this can be done?
Spectral matching is the obviously required for efficient FRET. But for FRET between Quantum dots (donor) and dye (acceptor), what is the role of surface functionalization. For example if one is amine functionalized and other is carboxy functionalized or both are carboxy functionalized, what would be the possibility of FRET, acceptor emission enhancment and acceptor emission quenching?
Thanks for your answers.
I am studying the effect of extended ultrasonication on the synthesized graphene oxide nanosheets ( specially on the activity of surface oxy-functional groups ).
I am looking for superparamagnetic nanoparticles with a polymer coating for our experiment. They can be in submicron size. They don't need to have surface functionalization as we have our own method to do that. If you know any supplier or your research group can provide this type of nanoparticles, please let me know. Thank you very much.
I want to clean "Gold Coating Particle from the PDMS surface". Is it Is "Ultrasonic cleaning with Ethanol" ok to do this task? Please suggest a suitable process.
I am Functionalizatng the Silica Nanoparticles with Various Mono-Alkoxy Derivatives such as methoxydimethyloctylsilane, methoxytrimethylsilane using the process of ultrasonication.
I want to know that how ultrasonication leads to the surface functionalization of silica nanoparticales with the chains of methoxydimethyloctylsilane or other Mono-Aloxy derivatives?
Only mono-Aloxy derivatives can be used for the surface functionalization in ultra sonic processing?
For the making of the super hydrophobic surface, we need contact angle greater than 150 degrees. For that which silane gives the best contact angle? how will NH2 affect the hydrophobic character IF a silane with NH2 is used? like amino propyl tri methoxy silane or 3 amino propyl tril methoxy silane
Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity
Free download via link below or please contact me.
The plasma modification of biodegradable nanofibers is of great interest for improvement of their biocompatibility. However, there are no systematic studies regarding the influence of plasma polymer deposition onto the surface of nanofibers to improve cell adhesion. In the present study, homogenous and reproducible modification of polycaprolactone (PCL) nanofibers by amine and carboxyl/anhydride groups was achieved. The concentration of amines NH2/C and C(O)O contribution were up to 2.9 and 14.1%, respectively. Regardless the plasma conditions, the deposition of amine and carboxyl-anhydride plasma coatings onto the PCL nanofibers sufficiently improved the cell adhesion and viability, as was evidenced by microscopy observations and ATP assay results. It should be emphasized that the deposition of negatively charged carboxyl-anhydride coatings resulted in slightly better cell adhesion compared to the positively charged amine plasma coatings, unlike the widespread opinion that COOH modification has less effect on myoblasts adhesion
We use oxide nanoparticles for human cell studies. We functionalize nanoparticles with silane group and Alexa Fluor 488 dye. However, the signal of fluorescent dye is not enough to follow the nanoparticles in cells.
I have observed in my study that post chemical treatment of a biomaterial, the intensity of the peaks (IR) has increased compared to untreated. The groups corresponding to the peaks are amines, carbonyl, carboxyl, and esters. If the chemicals used are sodium hydroxide, nitric acid or calcium chloride (independently) what could have caused this increase in intensity? Does it involve any chemical reaction with the existing groups? If so what (to be exact)?
Consider the biomaterial to be a lignocellulosic material.
Any answers are welcome. I would be grateful if chemical reactions could be given to explain the reason involved.
i am trying to make oleic acid capped iron oxide nanoparticles. but there comes washing problems.
my method was
1. reaction mixture 10ml+ ethanol30ml mix
magnetic separation. decant supernatant.
2. add little ethyl acetate to remove remained 1-octadecene(reaction solvent). decant ethyl acetate.
3. dried over high vacuum for 1h.
4. tested dispersion ability in n-hexane, chloroform , and cyclohexane.
sometime they were dispersed well in nonpolar solvent, but somtimes they didnt be dispersed in nonpolar solvent.(Agglomerated)
is there any precaution when i wash nanoparticles? i check TEM image and found nanoparticles were well made.
I am working with an alcohol solution saturated with LiOH and I need to make precipitate LiOH on the Silica surface, in particular I would get an uniform coating of the nanoparticles that I can not reach using just Silica nanoparticles without any functionalization.
So I think that the Silica nanoparticles functionalization could be helpful for my purpose, because I need to increase the affinity of the nucleating specie (Li+, OH-) toward the Silica nanoparticles surface.
The uniform coating can be obtained if I will be able to increase the nucleation points on the Silica surface instead of the growth of the few LiOH nuclei formed on the Silica surface; since the nucleation points on the Silica surface increase if the affinity between the LiOH and the surface is higher, I guess that by means of the silica nanopartices functionalization I could reach my purpose.
Could you suggest me some papers about the adsorption of Lithium ions and/or OH- on functionalized surfaces? or some paper which could be helpful for my purpose?
Is someone (industry, research groups) interested on -COOH functionalized silica nanoparticles? I know that several commercial sources are available, but they seem to be expensive for just a few miligrams. Would they have demand or usefulness if they were cheaper? Is someone interested on them?
i want to use the polyamic acid to conjugate ti antibodies using the EDC/ NHS coupling system.
I would like to know if there are any publications or any method to calculate partial charges of C,O, and H in functionalized graphene. I have done some literature search but could not get any information. I am doing molecular dynamics simulation of functionalized graphene. We know that in graphene C is treated as chargeless sphere but what about C,O,H in functionalied graphene?
I would appreciate any help.
I am working with a tribo geometry for food studies and wish to characterize the properties of the pdms surface.
The polymer with CH3O(CO) end group should coat the surface of MNPs, which are functionalized by NH2 group by preparation of amide band.
How to recycle solvents (ester) polluted by cadmium 2+?
I have one suspension of Solvent + cadium2+. Are there some way to separate cadmium? Filter sistem? or Chemical system?
thanks at all ...
Hello, I am trying to perform ELISA on glass slide; however I need to do carboxyl group activation of glass slide for Antibody immobilization.
I am having thioglycolic acid (TGA) in lab and have done bioconjugation of dots to antibody using TGA. So, was thinking to go for the same chemical for antibody attachment to glass slide.
Can anyone suggest me if this will work for carboxyl activation of glass slides?Thanks
Does anyone have insight into modeled or experimental work that studies the density of water molecules at hydrophilic surfaces that are exclusively functionalized with specific oxygen containing groups (i.e. ketones, alcohols or carboxylic acids) to assess the functional groups oxidation state on water hydration layer formation?
Thank you for your help in advance.
I have synthesized TOPO coated quantum dots in toluene. I want to make it water soluble. I tried phase transfer technique. I used 3-MPA to transfer in to water. When I add water in organic medium containing particles, it immediately looses luminescence. It lost luminescence even with the addition of MPA alone after some time. I thought this might be due to poor surface passivation. I tried to synthesize particles in presence of TOPO and Octadecyl amine. However, the particle yiled is very low and the luminescence of particle is very poor or none. How can I get rid of this problem. Thanks in advance for suggestions.
Currently, I am synthesizing the gold-coated silica ( 0.5mm < d < 1mm) for GEM (gaseous elemental mercury) analysis. We have successfully synthesized the product that matches the physicochemical properties described in numerous preceded researches. Our work is mainly based on the paper I attach below.
The next intention of our group is to co-precipitate PtNPs (platinum nanoparticles) alongside with AuNPs to create a Pt-AuNPs coated silica product.
I myself have referred to the one-pot synthesis of Pt-AuNPs where they use ascorbic acid-meditated reduction for the [AuCl4]- and [PtCl6]2- mixture, and I will use the procedure for the nanoparticle synthesis. I'm just not so sure the newly-formed Pt-AuNPs would be grafted on the silica surface, using ATPMS ((3-aminopropyl)trimethoxysilane) 1% v/v in methanol as the adhesive.
I am looking to hearing your opinion and willing to respond to any answer.
Thank you so much in advance.
I planned to coat certain antibody on the glass surface through EDC, NHS, (3-Aminopropyl)triethoxysilane. Before using antibody, I would like to try coating protein like albumin, lysozyme on glass. The question is, how do I show that those albumin, lysozyme are present and are covalently linked on the glass surface?
I need to choose a quite unique or new nanoparticles to increase membrane hydrophobicity. As far as I know, functionalized TiO2 and SiO2 have been widely used. The answer to the same question about a unique or new polymer is also appreciated. I would be grateful if you could share your ideas.
I'm looking for 200nm-500nm Magnetic Beads / Nanoparticles functionalized preferably with streptavidin.
Most particles I've found are 'cluster-type' based beads with a very high dispersion of sizes.
Do you know companies that provide these?
I want to do confocal microscopy of nanoparticles with free carboxylic groups on the surface, what would be the best fluorescent dye with free amine group?
I was trying to hydrophobically modfiy a couple of polymeric surfaces using silane treatment. While they do show a higher contact angle for water after the treatment, they continue showing affinity for alcohol mixtures. Is this an expected phenomenon? I presume they continue liking alcohol due to its organic nature.
I was looking for information about surfaces/ surface modifications that can repel water and alcohol (ethanol or propanol) but not other organic compounds. Can someone please share some information about the same?
It is easier to machine the irregular microstructured surface than the regular one, thus leading to a lot of unknown surface function for advanced application.
I am thinking of this : surface coverage = (weight loss of modified NP – weight loss of non modified NP) / (weight loss of modified NP). Thank you
Cathodic cleaning which takes place in GTAW with AC polarity helps in welding reactive metals as during DCEN cycle the surface oxide layer gets cleaned and during DCEP cycle welding takes place. Why cant we achieve the same in other arc welding processes?