Sugarcane - Science topic

Has anyone experienced difficulties during laccase assay measurements?

I have an enzymatic extract from a solid-state fermentation of sugarcane bagasse. The extract is dark, which interferes with the use of ABTS as a substrate for measuring laccase activity.

The ABTS is taking an unusually long time to be oxidized (more than 24 hours). I have already tried dialysis, but it still hasn't turned green or blue.

Does anyone have any recommendations?

I have an inquiry regarding nitrogen nutrition in sugarcane, and I trust that experts in nutrition and sugarcane research may provide insights.

Many sources indicate a positive correlation between the moisture content of sugarcane sheaths and the percentage of nitrogen in the leaves. However, I have not observed any significant positive or negative impact of foliar nitrogen on pod moisture levels.

In one of my experimental designs concerning sugarcane nutrition, I recorded multiple measurements, and the treatment with elevated leaf nitrogen concentration exhibited increased pod moisture.

If my findings are accurate, what supporting evidence do you possess regarding this matter?

Furthermore, can the beneficial influence of nitrogen on root development and enhanced water absorption be considered a valid rationale?

It is important to note that all environmental conditions, soil types, and irrigation methods were consistent across both treatments, with the sole variable being the quantity of nitrogen fertilizer applied.

For concrete mix design, which is the correct method in calculating the weight of the supplementary material for (1:2:4) mix ratio at 5% replacement with SCBA? Given the specific gravity of cement =3.1, sand = 2.6, gravel = 2.75, sugarcane bagasse = 2.8

a) Total volume = 1m3, Volume of cement = 1/7 = 0.143, Mass of cement = 3.1*0.143*1000 = 443. Weight of 5% SCBA = 0.05*443 = 22.15kg

b) Total volume = 1m3, Volume of cement = 1/7 = 0.143, Mass of cement = 3.1*0.143*1000 = 443. Weight of 5% SCBA = 0.05*0.143*2.8*1000 = 20.02kg

I know the term Co-generation and CHP are similar things. I used the term Co-generation in one of my research papers. However, I got a comment from a reviewer asking for using CHP instead of Co-generation. So I would like to know, is there any difference when the use of those terms in different situations.

Fertigation has becoming a common technology adopted by the farmers under protectively cultivated and protectively irrigated situations. Many companies dealing with plant nutrients are prescribing huge quantum of nutrients specially for crops like tomato, sugarcane etc. Is there a technological rationale / approach behind this heavy nutrition?

is it good to use mixture of sugarcane bagasse and molasses for mixotrphic cultivation of microalgae

Dear experts,

I am looking for some ways to speed up the formation of Sucrose in sugarcane by using specific nutrients (and their optimal levels) in fertilizer.

More specifically, can we produce a fertilizer which speed up the formation of Sucrose in sugarcane? If so, what nutrients (and in what optimal levels) should we combine?

Kind regards,

How can be use Sugarcane bagasse and filter mud to improve biomethane production?

Why Sugarcane is given FRP instead of MSP?

Hi everyone. Is the use of Phytagel better than agar in Plant tissue culture? I work with stevia and sugarcane, does anybody have experience in these crops in vitro? Or does anybody prefer the use of agar instead of Phytagel at any other crop? Why?

Thanks in advance.

If the dried yeast and activ dried yeast add externally in fermantion it can be obtained in yeast type saccharomyces cerevisie.

Hello I am an undergraduate and currently we are doing a thesis. Our sample is sugarcane leaves, can you recommend what is the best temperature and duration to oven-dry sugarcane leaves sample? And to achieve the constant weight of the sample?

Thank you!

I am encountering some challenges in the process of conducting a primary selection that can effectively distinguish the target DNA bands between pest -resistant and pest-susceptible varieties against the top borer. Despite my efforts, which involved using 1 primer and 3% agarose gel, as w ell as 1 primer with 3 different restriction enzymes, I have not ye t achieved a specific target band pattern on the 3% agarose gel. I would greatly appreciate any information or guidance regarding the issue I am facing. Additionally, I am seeking information on the resistance criteria of sugarcane varieties based on field observations. The resin stant sample also includes the use of wild sugarcane (S. spontaneum) in the first lane of the gel. Your kind assistance would be invaluable in helping me address these challenges. Thank you in advance for your consideration and support.

The question is raise in my mind is due to several factors like it is different for the variation with ecological condition?, lower number of variety, breeding concept/issue, management, etc...

In general my question s forwarded as 'How many varieties shall I use for characterization of sugarcane for agronomic descriptors and morphological parameters on lowland areas to recommend a variety which will be revealed as an opponent over the others?'

While transferring the plantlets in the glasshouse, all the sampling of sugarcane are drying in 1-3 days of planting. It has not any problems before rooting at growth room in the laboratory. How can I save these plantlets at the glasshouse? Do you have any suggestions? Please write me answers?

I am facing the severe bacterial contamination after 2 to 15 days of sub-culturing of sugarcane as this is noticed as yellowing of leaf in attached photo. Can anyone help me how can I save these plants. Photo is attached below

I have engaged in callus culture since many years, I have interest to know their medicinal properties. If it is, I can use it for other purposes. Sugarcane, plant tissue culture, laboratory practice, callus, nature of callus, Callus induction.

I´m investigating the production of glucose from sugarcane bagasse, and I really need information on the enzymatic hydrolysis reaction of sugarcane bagasse for reactor scale-up design.

I have a undergraduate research study. For that I need a explanation about this question

For spraying 0.1% bavestin on plant let's of sugarcane, tissue culture.

Checking of sugarcane production and productivity by tissue culture method, I want to know the answer. As I read and study, this techniques couldn't create genomic modifications. Please, share your views?

Sugarcane filter cake is one of the sugar industry waste which produce in a large quantities and has a potency to process as a biomass. What is the future idea for the utilization of sugarcane filter cake in industry?

What's the casual pathogen caused this symptoms on sugarcane plants?

1. Has anyone ever used Ants (crazy ants) for management of white grubs in sugarcane crop?

2. Is there any pheromone for Cochliotis melolonthoides Anomala spp and Heteronychus spp?

Replacement Value of Sugarcane Tops for Natural Pasture hay in the Basal Diets of Fattening Fogera Bull-calves at Andassa Livestock Research Center. In which journal is this paper published?

Is there anyone working on sugarcane tissue culture, shoot tip culture ?

I have been doing diversity analysis of sugarcane crop using SSR markers can someone tell how to analyse the gel documentation photos and what is the need for diversity analysis using markers?

I have done DNA isolation of sugarcane every time I don't get any bands of DNA can anyone tell me what could be the reason for this and why the bands are appearing like this?

I have done DNA isolation of sugarcane but it seems there is a problem in the bands(upper row) what could be the reason for this

Please give your advise on soil chemical mineral requirement like NPK, Ca, Mg,pH,EC, Micronutrient etc

Hello,

I frequently take soil samples for analysis to schedule fertilizer recommendation for different crops. Mostly, I take samples from 0-15 cm depth.

Is this is an ideal depth for all crops? how does it work with deep rooted crops/ tree crops/sugarcane crops (ratoon)? Also, how does the acidic sub surface soil influences plant growth for deep rooted crops?

Thanks

Attached photos are clicked by me during the field visit to the drip irrigation farmer, As per my knowledge Drip irrigation is done in the root zone to get proper benefits from it. But the farmer was saying sugarcane's roots are spread in the farm and it will absorb water. I gave him detailed benefits of drip irrigation method in terms of saving water, reducing weed by proper method of drip, power, fertilizers, etc. Also he satisfied with the same. Also suggest me is it right method by farmer?

I'm preparing nanoparticles using sugarcane bagasse. the method for its preparation include boiling of sugarcane bagasse. I want to know how boiling effect bagasse composition or what changes occurs in bagasse after boiling.

We know rice is a C3 plant. In context of rising global temperature, C4 plants (maize, sugarcane) are likely to have some physiological advantage over c4 plants. Rice scientist have been working to develop c4 rice varieties. What is the prospect of c4 rice varieties in rice growing Asian countries?

Some farmers use to do foliar applications of sucrese (sugar) and molasses in plants addressing improvements in plant health and increasing yield. Does it make sense physiologically?

I have taken the data from a field trial established to screen the sugarcane varieties for sugarcane grassy shoot diseases(GSD). RCBD with three replicates was used to establish the trial. Standard varieties are not available for GSD. Therefore, comparison and rating will not be possible.

The number of GSD infected clumps (phenotypically) and # of total clumps had been taken as the main data of the trial in the one-month intervals from 1 to 12 months (12 disease counts). Disease incidence was calculated. In addition, yield data were taken.

1. Could you please explain what kind of statistical analysis is suitable for analyzing the data taken here in order to find the varietal response for the GSD by the SAS program based on disease data?

2. What data (# disease clumps or disease incidence) is appropriate to use for analysis? Further, it would be a great support if anyone can give an idea of how to write CLASS and MODEL statements of SAS for analyzing this data.

Thank you

I am trying to estimate hemicellulose percentage in a lignocellulosic biomass like rice straw, sugarcane bagasse.

Sugarcane and sugar

Given the chemical treatment applied to sugarcane bagasse in adsorption of heavy metals how can I exclude the effect or factors that the chemical modification affects?

Diaz, P.. (2016). Consequences of Compost Press Mud as Fertilizers. DJ International Journal of Advances in Microbiology & Microbiological Research. 1. 28-32. 10.18831/djmicro.org/2016011005. Organic fertilizers derived from Sugar Press Mud (SPM) yields better production of crops. Sugar press mud or the sugarcane filter-cake is the residue of sugarcane industry which results from the processing of sugarcane where sugar mud is separated from the crush. The total supply of sugar press mud varies from (1-7) kg from the processing of 100 kg of sugarcane. Sugar filter cake is used as a suitable fertilising agent since it is rich in micro and macro nutrients along with organic carbon. It is eco-friendly and protects the plants from various soil borne diseases. Press mud compost does not include any substances which are unfavourable for microbial action. In certain cases, press mud is mixed with other organic fertilizers to yield enriched compost. Whatever the case, the resultant press mud should not be added to the soil directly as it affects the welfare of human health due to the fast growth rate of pathogenic fungi. These are the disease causing microbes whose growth rate must be inhibited. The review deals with the physical, chemical and microbial functions on the press mud resulting in excellent bio-fertilizers. Enriched press mud is mixed with gibberellic acid to inhibit the growth of toxic chromium which affects the metabolism of plants. The fields which are ineffective by the over use of chemical pesticides can be brought into control by constant use of bio-fertilizers such as press mud. Usage of chemical pesticides may destroy the insects causing damage to the fields, but its impact over the health of living beings is tremendous. Press mud, on the other hand, is safe and very effective in producing income and killing certain insects affecting the crops as well.

Good practices during preparation, fermentation and distillation can reduce the concentration of acetic acid in distilled spirit to keep the standard quality of final product. However, I want to ask if is there any corrective measures to reduce the high acidity found in the product after processing?

I'm preparing graphene oxide using sugarcane bagasse powder. Method of preparation is: take 0.5g sugarcane bagasse in china dish and add 0.1g of ferrocene as an oxidizing agent. Mixed them and then put into muffle furnace at 300 °C for 10 mint.

Dear colleagues, the next goal of my project consists in the phenotyping of sugarcane lines under water stress. Could you please share some fundamental scientific reports or advices about the topic to take in account before starting to work on this. Thanks.

I am working in a sugarcane TISSUE culture lab and my cultures shows stunted growth and leaves rolling down.Why this is going on?

I want to use freely available remote sensing datasets (multi-spectral) to assess actual leaf nitrogen content.

Sugarcane crop remains in the same field for 2-3 years. Cynodon dactylon and Cyperus rotundus are the main weed of sugarcane based cropping system. Kindly suggest any non chemical weed control measures for control of these weeds. Hoeing is generally recommended but regrowth take place within 2-3 days.

If glyphosate is used after sugarcane harvesting, what will be the effect on the germination of ratoon crop?

Many farmers in subtropical India grow sugarcane in spaces between Populus tree during first 2-3 years of Populus plantations. They are facing difficulties in selecting the herbicides safe to both the crops. Atrazine is safe to sugarcane but not safe to Populus tree. Kindly suggest pre or post herbicide being safe to both sugarcane and Populus tree.

Good day. I am looking for references on plant tissue culture medium for my study. My study right now is on fermented solutions derived from plant wastes as a replacement for the medium in the plant tissue culture. I already read flyash and sugarcane vinasse, but I need more literature. Hope you can help me and also give suggestions for my study. Thank you.

Hello everyone,

I've been struggling with this matter:

We have data on sugar cane production (soils, treatments, weather …). We want to understand in which conditions soil/plant nutrition will yield the best result. It is hard to infer causality as some treatments are often performed jointly and we are not able to isolate the partial effect of the applications of interest.

Any one has any leads on interesting statistical methods please ?

Thanks a lot

Per month water requirement of wheat, paddy, potato, maize, sorghum and etc is high in comparison to sugarcane.

Tiller dynamics in Sugarcane.

Kindly provide the information for use of cutter planter and harvester etc in sugarcane.

Sugarcane is a vegetatively propogated crop.

Embryogenic callus masses treated with ethyl methane sulphonate (EMS) in proper concentration and selected on medium added NaCl in in vitro condition to gain stable mutants with salt-tolerant character. After that, those are evaluated in ex vitro condition for agronomic characters. Could anyone suggest me a good procedure for carrying out?

It is needed for calculating the resource allocation and planning of the crop for planting in sub-tropical India.

Due to high cost of production, sugarcane is becoming less remunerative crop. Hence crop diversification in sugarcane farming systems is one of the important strategy which can increase the net returns of sugarcane farming.

I want to develop a method to preserve sugarcane juice in any form.

Hello all,

As part of my Ph.D. wok, I am carrying out Co-pyrolysis of biomass (sugarcane bagasse) with HDPE. For the same, I have carried out TGA kinetics experiments for various ratios of biomass and HDPE (1:0, 1:1, 2:1, and 4:1) under inert atmosphere. From the TGA thermos gram, I had evaluated the activation energies of sugarcane bagasse using iso-conversional methods such as Friedman, Flynn–Wall–Ozawa, and Kizzingar-Akahira-Sunrose. Through these methods my calculated average activation energy turns out to be 207.56KJ/mol,232KJ/mol and 203.1858 KJ.mol respectively. Even though these values are somewhat consistent, they are higher than the reported value in the literature. What will be the main reason for these higher values? Is it normal to have these higher ranges? Any insight to arrive at a meaningful conclusion for publication of these results will be of great helpful.

sugarcane produce more tiller because of that we cannot maintain equal spacing hence how we can calculate crop growth rate for sugarcane when we do research on different spacing

The crop is of 40-45 days old at farmers fields. The farmers have applied two different unapproved combinations of herbicides and insecticides. First unapproved combinations was application of Atrazine @ 5.0 kg +2,4- D amine 1250 ml/ ha ( products dose) herbicide in 40 Days old crop in standing water of about 10 cm. Another unapproved combinations was application of Thio+phorate+carbendazim in plant and ratoon crop. Can anyone help me in distinguishing the exact cause of these symptoms.?

My polymer is epoxy and fiber is sugarcane

Hi all,

I was explaining the plant schedule of crops to a coworker and was describing the purpose of a fallow year as a way for the soil to rejuvenate. In the case of sugarcane, I know it is common for the ground to be consistently tilled in the fallow year to avoid new growth. My coworker asked me why they don't just plant a cover crop in the third year to avoid tilling and I honestly don't know the answer to that.

Any help is appreciated!

Keeping in the view, some suggestions are prepared for growers/farmers of Sindh to overcome the possible threat.

Suggestion for Crops should be cultivated and not cultivated during current Kharif season 2017

I have suggested following crops to be cultivated

Sorghum (Jowar)

Millet (Bajra)Maize

Mungbean (Green gram)

Mash (Black gram)

Cowpea

Sunflower

Soybean

Groundnut (Peanut)

Sesame

I have suggested following crops should not be cultivated

Cotton

Rice

Sugarcane

Please add your suggestions

For ratoon crop without destroying the bunds

1. what is the growth rate of farm mechanization for sugarcane farming?

2. its statistics ?

3. Future potential ?

Sugarcane + Onion were planted on same day. Onion was planted on raised bed whereas sugarcane in furrows. Sugarcane setts were covered with 3-4 cm soil. Pendimethalin herbicide was sprayed on soil surface after two days of onion transplant. Whether sugarcane germination will be affected due to heavy rainfall on next day of spray? We suspect that pendimethalin may come in direct contact of sugarcane buds.

I'm looking for ranges of Nitrogen and Phosphorous in Sugarcane leaves and stalks.

I need to know the protocol for measurements Specific Surface Area laboratory for sugarcane bagasse

Sugarcane and onion seedlings were planted in mid January. Pendimethalin was applied as pre-emergence two days after planting. Kindly suggest post emergence herbicides which are safe to both the crops.

usage and utlization and collection of sugarcane straw

Sugarcane is a member of the grass family and is valued chiefly for the juices that can be extracted from its stems. The raw sugar that is produced from these juices is later refined into white granular sugar. As a sugarcane plant matures throughout the growing season, the amount of total sucrose in the cane increases. Most of this sucrose production occurs when the plant has fully matured and begins to ripen.

Stubble shaving, off-barring and fertilizer application for ratoon sugarcane guidelines

What areas of the world, mites are one of the sugarcane pests?

Hi I currently doing a research proposal using sugarcane bagasse in some pretreatment and I want to measure the composition (cellulose, hemicellulose, lignin, ash) using this method (Hashabra et al. 2017) link:https://www.google.com/url?sa=t&source=web&rct=j&url=https://pdfs.semanticscholar.org/55a7/72c4f0600c9bbc054aa8e30dcfaa14ef592b.pdf&ved=2ahUKEwjwwtKYqOblAhVKYo8KHQa3BLYQFjAAegQIARAB&usg=AOvVaw160UFDAuf8uIhNG-F-uRXj

Hashabra et al use 1 g of sawdust to measure the composition, but my advisor think it is too small amount. Maybe because different kind of substrate? Any recommendation how many grams bagasse do I need to take to measure the composition?

I also want to see the structure using SEM, FTIR and XRD. Any recommendation how many grams sugarcane bagasse for each SEM, XRD and FTIR?

Thank you before