Science topic

Sudden Infant Death - Science topic

Sudden Infant Death is the abrupt and unexplained death of an apparently healthy infant under one year of age, remaining unexplained after a thorough case investigation, including performance of a complete autopsy, examination of the death scene, and review of the clinical history. (Pediatr Pathol 1991 Sep-Oct;11(5):677-84)
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Oil massage to newborn in India is practiced since Centuries and especially mentioned in Ayurveda. Which provides tactile stimulation to newborns and infants, also improves blood supply and reduces stress, results in better sleep (one of the importance factor for normal secretion og HGH). One of the theory established in pathogenesis of SIDS is chronic low grade hypoxia. Countries like USA are having more incidence of SIDS, where such practices are minimal. Can we hypothesized that Oil massage plays important role in preventing SIDS.
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Why don't we conduct an experiment on neonatal rabbits for instance to prove/disprove that claim?
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Hi everyone!
I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol (https://www.thermofisher.com/order/catalog/product/AM1561?SID=srch-srp-AM1561#/AM1561?SID=srch-srp-AM1561) says that I should use phenol:chloroform (125:24:1) but we have plenty of phenol:chloroform (25:24:1) at the lab.
Some colleagues said it should not change the outcomes of the RNA extraction but just to make sure. Does anyone know if there is any difference between both concentrations?
Thanks a lot!
Ana
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Hello, I think it will work too, but to be sure for extraction efficiency better to compare the extraction yield of at least 3 paralel samples by both with and without solvent modification and compare data by t-test.
Good luck!
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Hello everyone,
I am working on the topic; Impact of Income Diversification on the Livelihood of Small Holder Dairy Farmers. One of my objectives is to evaluate the determinants of income diversification. I have seen related articles on this topic in which the researchers used tobit regression because of the presence of zeros in their dependent variable; SID.
I tried carrying out the regression in STATA but all I got was just the coefficients. The std. Err, t, p values and confidence level are blank.
What do I do please?
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Please tell more about your problem, at least the number of observations, the number of explanatory variables, if these variables are really linearly dependent. The symptoms mentioned correspond to a degree of freedom equal to 0.
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RNA Fragmentation Reagents
The average size of the resulting fragments will be 60-200 nucleotide. What is the mechanism? How to control the size?
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thanks
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I seed 2 X 10^6 cells in each well in a 12-well-plate, and I tried 10, 50, 100, 150, 200 ng/mL PMA for 48hr, but the differentiation efficiency is too low that I am unable to do the downstream experiments using THP-1 derived macrophage.
+ 50μM 2-Mercaptoethanol +1% Gentamycin.
Thanks for your reply!
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Interesting question with great insights. Looking forward to the discussion!
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Recently I bought this from Thermofisher. After performing the RNA isolation according the manufacturer's protocol, I got the bands like this. Could you please clarify me if this is Total RNA or not?
Thank you!
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Hi Kanaka, yes you can, as this kit is designed to extract both the total and smaller RNAs. As far as, you get most of the aqueous organic phase carefully without contaminating with lower interphase, and then proceed to total RNA protocol, it should be fine. Take care about the temperature of absolute ethanol, as it must be maintained at room temperature, also use fresh new bottle of ethanol as it is being very hygroscopic hence gets diluted upon log term storage. mirVANA PARIS kit is not compatible with spleen and pancreas tissues as per the manufacturer guidelines. Never spin filter cartridges above 100000 g, it seriously may damage filters, which I have personally observed. Preheating of elution solution at 95 C is very critical step in PARIS kits, so take a good care of it. If you have Qubit fluorometer so measure your RNA via HS or BR Qubit RNA quantification kits, as these will give you more authentic quantification of your RNA than the nanodrop, and while quantifying RNA with nanodrop focus well on your 260/230 ratios, which should be at or above 1.7 for all of your RNA samples.
Regards....
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I had a look at different protocols for RNA isolation and I am a bit confused about the the best way to do this...
let`s say I do a classical Trizol extraction (on bacterial cells), then treat my aqueous phase with DNase I.
One protocol says to leave the DNase I for 2 hours at 37 degrees, then re-do a Phenol/Chloroform extraction to get rid of it (without heating at 65 and without EDTA).
  1. That sounds good (2h could be replaced by 30 min, though) but will I not loose too much RNA by redoing the extraction (considering I expect my gene of interest to be very lowly expressed) ?
  2. I could also choose to heat inactivate it without re-extracting RNA, but I might degrade my samples...
  3. Thus I was thinking that I could use the ezDNase instead (https://www.thermofisher.com/order/catalog/product/11766051?SID=srch-srp-11766051) because it is active in 2 minutes at 37 degrees and can be inactivated in 5 min at 55 degrees (or left in the mix without inactivation apparently).
  4. ...but would I have more contaminant proteins ?
Basically, I am wondering what I should prioritize during my extraction step, knowing I am working with low levels of target mRNA.
Thanks in advance for your help !
Katy
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By heat inactivation and treating with proteinase K followed by phenol:chloroform extraction
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Its a Literature review
Can someone help me with putting this into PICO tool? I have tried PEO and PICo but still working on it. Any ideas, please
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OK thank you Farah Nabil.
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Did anyone have the experience of using NE-PER kit for extracting the protein following by determining the protein concentration by ELISA? I'm wondering that is there will be any interference? The description of this product only mention that it can work with enzyme activity assay. And didn't see any citation linked to this product do ELISA as downstream assay. Please provide me any suggestion. Thank you.
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No
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The review of the international literature on the hexavalent vaccination adverse effects, documents, without doubt, the existence of a new clinical syndrome: “The Post Hexavalent Vaccination Sudden Infant Death Syndrome ”, (PHVSIDS) without concurrent pathologies inducing a life risk confirmed by autoptic investigation, with an enhancement of the infant mortality and hospital admissions rates correlated to the number of vaccines before the first year of life.From 1999 to 2004, in Italy, there has been a slaughter of 52 infant deaths directly associated to hexavalent vaccination , 8 before 24 hours (RR= 1,5, RR= 2,3 with the Infarix Hexa vaccine- 0,7 with Hexavac ) , 34 within 7 days (RR=1,8- RR 1,5 with Infarix Hexa- 2,8 with Hexavac, with all the hexavalent products RR =2) 52 within 14 days (RR 1,5, RR= 1,5 with Infarix Hexa,1,6 with Hexavac). Children’s deaths notifications continued after this date. The hexavalent vaccination appears like a Russian Roulette in children with an unknown vulnerability. In addition to PHVSDS, there is the clinical and scientific evidence of auto-immune diseases, associated to vaccinations that derive from minerals added to vaccines as adjuvants, preservatives and stabilizers, like Aluminum , inoculated in enormous quantity, and Mercurium, that allows to define a new clinical Syndrome: the Post Vaccination ASIA Syndrome-PVAS). ASIA is the depicted Autoimmunity Syndrome Induced by Adjuvants . Moreover, there is an irrefutable documentation that hexavalent vaccination determines immunosuppression well depicted by immunology, a consequent cause of death from other infections, as happened .
The philosophy: " “ Let few deaths for saving much more lives " is unacceptable for a clinician, when in Public Health other preventive solutions are possible.
(From
The hexavalent vaccination Russian Roulette
The hexavalent vaccination risk for children’s and adults life . The introduction of The Post Hexavalent Vaccination Sudden Death Syndrome (PHVSIDS) and the Post Vaccination Asia Syndrome( PVAS). in Research Gate)
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I read the letter in which you show the proofs. Too bad.
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I am reviewing Fluoride Death Data including the excess deaths attributable to Fluoride Toxicity, including but not limited to: Alzheimer’s, Anaesthetic Hyperthermia, Aortic Rupture, Asthma, Cancer, Cataract, Chronic Kidney Disease (including Pyelonephritis), Chronic Obstructive Pulmonary Disease, Crime (including Fluoride enhanced Plumbosolvency leading to elevated Blood Lead Levels), Diabetes, Fluoridation overdosing, Eclampsia, Foetal and Perinatal mortality, Gels, Rinses and Toothpastes, Hip Fracture, Liver Failure (including Fat Burner and Tea products), Stroke, Sudden Infant Death Syndrome, Suicide. I have already found many references but would like assistance in building a comprehensive bibliography.
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Fluoride is known to increase the incidence of bone fracture. Older adults have a 5- to 8-fold increased risk for all-cause mortality during the
first 3 months after hip fracture. We can therefore safely say Fluoride causes excess deaths via this mechanism.
See this interesting article with references.
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We are compiling a toolkit of existing and tested tools for VCAs, ranging from rapid appraisals to in-depth assessments, that can be effectively applied in coastal and fishing communities to better understand local vulnerabilities to climate change in the fisheries sector. In particular, we are looking for recommendations for VCA tools developed and tested in small islands developing states (SIDS).
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I believe that you must first pay particular attention to sampling. Since everything depends on howmuch objectively and statistically programmed are the data collected. Therefore, perhaps you must also add a part dedicated to data collection methods.
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Hi All, my dissertation is about filicide... im researching the factors that are behind/contribute towards this from a biopsychosocial perspective... thankyou
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Dear Bethany, 
Here I send you some papers we wrote about maternal blues and postpartum depression.
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Is anyone actively researching Parechovirus?  It is anticipated that the virus will again see some outbreaks this fall.  Yet, I have not seen any US researchers or organizations publishing anything about its prevalence, effects, or precautionary advice for parents or parents-to-be.
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There is no clear evidence of the usefullness of home monitoring in prevent SIDs.
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Here is a publication that might be of interest to you. Central sleep apnea is the major cause of SIDS, which the authors of this paper aim to diagnose using signal processing and machine learning techniques.
This if put into practice and made commercial could perhaps help reduce the number of deaths due to SIDS
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It is well known that babies cry just before sleep. Sometimes the cries have specific origins that cause pain or discomfort. But as everyone knows, even when all those factors have been eliminated every baby still appears to cry in an attempt to fight sleep itself.
This question is NOT asking for a solution. This is a scientific question. I am asking why? Why do they fight sleep? Is there a survival advantage? Is there a physiological explanation? What research has been carried out into it to determine the reason for the "sleep fight"?
Could it be that a good cry before sleep actually has a physiological payoff that confers a survival advantage? Does it "prepare" the baby's breathing pathways before sleep, for example?
Are there some meta-analyses or epidemiological factors we can do to check this hypothesis? Are those babies that die of SIDS those that do not cry as much before sleep? Is there any epidemiological data to test such ideas?
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Crying stimulates milk production in mothers and milk contains chemical that promote sleep?
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There are a number of smartphone applications that use the phone's accelerometer to monitor sleep patterns. The user simply places the phone on the bed beside him or her, and the phone records motion during sleep.
Is this accelerometer sensitive enough to detect the motion created by an infant's respiration? If so, it would be a simple matter to create an app that would alert the parents if the child ceased breathing. Perhaps the alert would arouse the child enough to cause him or her to resume breathing.
An alternative strategy would be to use the phone's camera to detect the breathing motion in the infant. The phone could be placed beside the infant with the camera facing the ceiling. A laser on the ceiling could provide a stable point of reference, so that any motion of the phone would be detected by its camera. This might be more sensitive than the phone's accelerometer. Once the motion is detected, the strategy is the same: produce an alert if the motion caused by the infant's respiration ceases.
What do you think? Is this viable?
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The case of infants is very sensitive and the accuracy can not be compromised. But the question also arises whether the signal alert by a very slight movement of the infant is desired. Then again signal from the infant is desired to be real time. In that case, the sensors in smartphone or a small external transceiver can to used to send out the signal to another smartphone or an alarm device via the router in local area network. 
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There is an enormous body of literature citing the biotoxicity of aluminum (3+) salts, including neurotoxicity and cancer.
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@ Joseph - When you analyze the VAERS database comparing the incidence of reports of death in Al-containing vaccines against vaccines w/out Al, it becomes clear that all of the commercially-marketed Al containing vaccines have greater numbers of reports of death, sometimes sudden death. HPV vaccine is a case in point. Serendipity? Of course, association does not establish causality, but have you wondered why metallomics studies are showing high Al levels in samples from autism patients? More serendipity? Also, if causality between Al and sudden death was ever firmly established, that would make the century of Al-related deaths in substantial measure iatrogenic, yes? Our environmental exposure to neurotoxicants is ever increasing. Al is in the food supply, cosmetics, sunblocks, etc. It's also in upper outer quadrant breast cancers. More serendipity?