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Stress Response - Science topic
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Questions related to Stress Response
Hi everyone, I would like to check whether the ER stress response is activated in human fibroblasts. I am particularly interested in the ATF6 signalling pathway. Therefore, I would like to check the protein expression of the cleaved ATF6 by western blot. Unfortunately, I have problems finding a good antibody for this. My current antibodies only show unspecific bands. Do any of you have experience with this and could recommend an antibody or another method?
Many thanks in advance!
We're seeking collaboration to the actual cause of psychophysiological dis-ease. The animal kingdom shakes off stress after being threatened, so why can't we? Is it possible that humans have developed to such a level that stressors are stored extracorporeal, outside of the body and constantly trigger the stress response as a result. Our ability to store and stream data, that is vital to our survival may prove to be the difference that Darwin mist. The ramifications and applications of an informational health based model, will shake the very core of current Western medicine. Ascribing the disease within human-beings to be a dis-ease with their environment rather than, the persons health is defective, is surely a better way for us all to be searching for? Please get in touch to collaborate with a natural, evolutionary approach to physical and mental wellbeing.
I am interested in learning the relationship between the glucocorticoid hormones and stress response in fish species, and understanding the mechanisms underlying variation in GC levels.
thanks in advance..
If stress cause depression but individual adapt to decrease dramatically depression symptoms in the cost of anxiety appearance in this case it is active coping or maladaptation or what this situation can be described ?
Hello everybody,
I am writing a UMAT for abaqus, a small strain perfect plasticty code. When i run my code for 4 node single element plain strain condition, i get a stress response higher than my yield stress. However, i get exactly the yield stress when a 3D single element is used. Hence, i am wondering what migth be the difference of same plasticty model according to 2D single element and 3D single element and why is 2D stress response is higher. I am adding a graph so that my question could be understood more easily. Thank you in advance!
Çağatay Kasar
I hear from many senior doctors- more than 20 - 25 years of experience- in residency training clinics and departments that a new generation of assistant doctors are on the rise.
Of course the generations change, and this is normal, but there could be interesting opinions.
Would you like to share?
- Have you realized behavioral patterns different from "the old times"?
- Have the expectations/stress responses/ increased work handling of assistant doctors changed recently?
- Is there a noticeable difference in the way the new doctors treat each other in their daily routines? Are the clinics more or less stressful nowadays?
- How about medical students?
I am running a trial to evaluate the effect of graded levels of some phytobiotics (black pepper, papaya seeds etc) in diets for broiler chicks during starter period on the activities of some immune enzymes (Catalyze, Superoxide dismutase and Glutathione peroxidase). I also wish to study the effects on the dietary treatments relative to the control the effects on stress response. I am thus seeking suggestions on easy, efficient and practical methods to stress 5 week old broilers so that I may evaluate the stress responses via serum cortisol concentration (if practicable).
Could anyone please suggest to me a method that they adopted or are familiar with that is practicable? I will cite the work if it is already in the literature.
Thank you.
Hydrogen gas has effects on a range of physiological events in plants. It has been shown to have effects on seed germination, plant growth, and development. It has also been found to be involved in plant stress responses and to be protective against pathological abiotic stress challenges. Similarly, it also has beneficial effects during the post-harvest storage of crops. Therefore, its use in the agricultural setting has great potential as it appears to be safe, with no toxicity or harm to the environment.
This Special Issue aims to bring together a body of papers that focus on the current state-of-play of the molecular biology and possible uses of molecular hydrogen with plants. It is hoped that this Special Issue will highlight the future work which may be undertaken in this field and help to encourage researchers to investigate this exciting field further.
For more information please follow the URL, below.
Hello everyone,
I have the spring stiffness and damper coefficient for the Kelvin-Voigt model and I need to calculate/fit (by using Prony series) the Instantaneous/infinite elastic modulus and decay constant, which are generally derived from the stress relaxation response. Since the Kelvin-Voigt is not able to predict the stress relaxation behavior well, do you have any idea how can I calculate those constants?
Best regards,
Shahab
I am wondering why the HPA mediated cortisol response only peaks after 20 minutes whereas SNS is quick acting. Is this because SNS is nervous system mediated and HPA is endocrine pathway mediated?
Hello, we are a group of students. This year we have a project which deal with the "study of the stress response of micro-organisms for the optimization of food production processes".
It's hard to find real infomations about it, so we need some help. Does someone has already work on this subject o one a similar subject ? or know someone who work on it.
Thank you for your help.
Hi,
I am looking at a change in protein expression after treatment over time and comparing samples to an untreated control. However, certain proteins i.e. stress response proteins, does not produce protein bands during immunoblotting in untreated samples. I was just wondering how I can calculate fold change or alike, with control samples effectively not showing certain protein due to the absence of stress.
Any relevant thoughts are welcome.
I started my PhD study about stress and daily activity and I need to find appropriate device for long measurements (+24). I've found two interesting devices so far: E4 wristband produced by Empatica (https://www.empatica.com/e4-wristband) and BioHarness 3 by Zephyr (http://www.zephyranywhere.com/products/bioharness-3). Did you use one of them during your studies? How do you assess their quality? Or maybe did you use something another?
I'm interested in measuring such signals as: EDA, heart rate and motion. Thank you for each proposition of device and your opinion.
Mateusz Soliński
XRD results indicate that there are significant peak broadening and peak shifting in cold rolled Ti64 alloy (10 % cold rolled).
1- Is it logical to relate peak broadening to the lattice strain during deformation?
2- which kind of stress is responsible for lattice strain ? (plastic or elastic)?
3- Is there any relationship between dislocation density and lattice strain value?
I need a method for postprocessing of fatigue damage, from the random vibration respons result. It should be done in the frequency domain, using proper statistics, for speed and it should be easy to implement. How do you do it? Please share your experience.
Often there is one eigen-mode that dominates the stress response. However, when that is not the case the method must be able to handle complex stress states, including multiaxial stresses with rotating principal directions.
One method that is tempting to use involves the scalar stress PSD, called the 'equivalent von Mises stress PSD' introduced by Pitoiset and Preumont. It is derived using the same quadratic formula as when calculating the common von Mises stress invariant, but using the quadratic operation on the PSD matrix elements instead. The resulting scalar stress PSD can then be interpreted as the the PSD for an uniaxial random stress and 'conventional' relations between PSD and fatigue damage can be used (narrow-band, Dirlik, etc.).
There is a lot of relevant papers to study in Procedia Engineering vol 101 (2015), including a nice review of 'spectral multi-axial fatigue analysis' by Benasciutti, Sherratt and Cristofori. Research is interesting enough, but what method do we find efficient in industrial applications?
Excluding pupilometry, is there any method, how to measure immedate stress response? First I was thinking about hearth rate than about skin conductivity. In both cases there is to long delay between occur of stressor and recorded stress reaction. Do you know about something else? Or how to get more accurate information from hr and sc? Perhaps some reaction in eye tracking?
Thank you very much
These are theories with which I'm familiar so far:
1) Attention Restoration Theory
2) Predictive and Reactive Control
3) Natural settings evoke meditative-like states (which in turn reduce rumination, which prompts a sympathetic nervous system trend towards a more relaxed state)
What other compelling theories are out there that specifically address (or at least explore) the neural, psychological and/or biological mechanisms by which interaction with nature helps to reduce stress...?
(Responses that include links to quality resources would be appreciated.)
Many thanks!
Catherine
I want to do single cell RNA-seq with yeast. Droplet-based microfluidic technology is adapted for mammalian cells and the lysis method is therefore unlikely to be sufficient for yeast. Therefore, I'll need to digest the cell walls and submit spheroplasts for scRNA-seq. The problem is that the transcriptomic signature I'm anticipating will largely involve activation of stress response pathways including the cell wall integrity pathway. I therefore need to preserve the cellular RNA prior to processing (including spheroplast prep, live cell sorting by FACS, and transport to scRNA-seq facility).
Any fixation method which permeabilizes the membrane (like alcohol) will be incompatible with my sort (dead cell stain like PI or SYTOX Green). Any fixation method which requires removal of the cell wall (like DSP) won't be sufficient to protect RNA during cell wall digestion. Reagents like RNAlater seem viable but I don't know how I would digest cell walls and sort cells all while constantly keeping the samples in RNAlater.
Any advice is appreciated!
I am a Crop physiologist and would like to understand stress responses of plants of Indian rice varieties that have been recently developed.
Hi!
In our psychophysiological stress research, we plan to measure 3 modalities: EDA, ECG, and temperature. However, we also consider EMG as a interesting stress marker. The physiological measurement will last about 20 minutes altogether.
Do you have some experience in measuring EMG (e.g., the activity in upper trapezius muscle) to index stress response to psychological stimuly (e.g., social evaluation, mental load)?
Would you recommend to use EMG signal in a multi-modal assessment of the stress response? Does it provide a valid and reliable measure suitable for indexing the presence (or degree) of acute stress?
Thank you for any relevant advice,
best,
M.
I am interesting to do work on prediction or searching of cis regulatory motifs (stress responsive) from genes(promoter region) in plant resources. so anybody can suggest me a good database of such motifs other than PLACE. or suggest any tool to efficiently predict moist stress/salinity stress responsive motifs or elements.
Thanks in advance.
Fatigue loading tends to damage the material after a particular number of cycles. Considering the material science and human body in a single frame, imagine a person cycles up a mountain everyday. His knees are exposed to fatigue loading, and is there slow damage happening to his knees due to this fatigue loading?
i am aware that gene expression profiles might differ between cell types and types of mechanical forces. But what are some 'consensus genes' which expression level changes (high chance) upon mechanical stress? i want to detect some gene expression changes to justify doing RNA-seq later.
Hi everyone,
Why is it indispensable to activate the ATF4 during amino acid stress response?
Dear fellow researchers,
I am attempting to carry out a thermal stress analysis on a 2d quarter cylinder part model I have attached in the image file. The geometry is in XY plane and I want to apply differential heating in the inner and outer radial surfaces to generate thermal stresses. I want the stress responses for a generalized plane strain configuration. However, the results don't match with my closed form solution. I am positive there is some mistake in my FEM model. Particular queries I have during the modeling are the entries I have attached in picture 2 file. Can someone please tell me the meaning of these fields. For my problem I would like the cylinder to expand freely with a constant strain in the Z direction.
I have a mutant strain that I have analysed by RNA-seq and when compared to WT, the differentially expressed genes seem to be following the pattern for the yeast environmental stress response (up-regulation of metabolic genes and down-regulation of translation genes etc). Is there a direct way in which I can test whether this mutant is inducing the ESR? for example the phosphorylation of a protein?
Thank you :)
It has been reported that excessive inflammatory cytokines such as IL-1beta, IL-6 and TNF-alpha in the serum secreted from the tumor tissues are responsible for the development of weight loss up to 5% decrease within 6 months, also referred to as cachexia. Hyper-metabolism in skeletal muscles and adipose tissues is characterized by promoted beta-oxidation of free fatty lipids in mitochondria. Enhanced beta-oxidation causes accumulated ROS. In addition, the cachexia cancer cells lead to the activation of p38-MAPK signal pathway in skeletal muscle cells. I wonder whether metabolite-induced ROS directly activates p38-MAPK signaling cascade, and furthermore, other stress-response signal pathways are related or not in the cachexic pathophysiology.
The Seahorse kits are so expensive. I was wondering if I could just buy the chemicals and use these in place of the kits. Specifically Oligomycin and 2-DG.
Hi, I want to use the acute social defeat stress but I don't know which strain should I order, the literature says in general only CD1.
Thanks!
I want to establish the high efficient cardiomyocyte differentiation system using mouse embryonic stem cell (mESCs). My colleague told me the hanging drop method (old and classical but famous one). This worked well a little bit but it was not enough efficiency (around 1-5%) to conduct further studies.
I want to research the stress response of mESC-derived cardiomyocyte. It needs more purity and mass. If you know other better methods, please tell me.
Is there any good cardiomyocyte differentiation system?
If anyone knows anything about salivary alpha amylase, I would be interested to hear your experience with collection and analysis.
Blood pressure will also be measured, but I am not sure that is enough of a stress response measure.
I have constructed a rail track model in ABAQUS using dynamic explicit analysis.
Axle loads are positioned at the start of the track and for a period of several seconds the load magnitude rises in amplitude until they reach the required value, to prevent a sharp jump in track inertia.
The stress response of certain layers in the track is fine initially, but suddenly the response becomes chaotic and very asymmetrical.
I have attached a few images of the ballast layer to show the response. Note that in all of the photos the load remains stationary, albeit the magnitude of the load is increasing from zero to maximum. The photos are from consecutive time increments with the same load step.
Thank you in advance.
Kind regards.
There is evidence that stress is mediating the effect of diet on body weight (Peters, A et al).
Is there also evidence on whether (reduction of) stress may be involved in the beneficial health effects of leisure physical exercise?
Good morning
I was analysing the literature on stress levels of different professional categories. Unfortunately, I found that the category I am most interested in are sampled for salivary cortisol; the other, which I hoped to use as a benchmark, are sampled for blood or urine cortisol. Is there any way of making findings with these different techniques comparable with each other?
Thank you in advance
We would like to design an experiment, in which degradation of a certain transcription factor leads to an optically visible (i.e. easily screenable) phenotype in Arabidopsis seedlings (about 3-5 days after germination). The phenotype, however, cannot be light-dependent and the expression of the TF needs to be inducible and not influenced by light either.
We were looking into defense and stress responses to find a good candidate, but havent had a very good idea so far.
Feedback loops are triggered at
various levels to shut off the axis through inhibition of the
corticosteroid receptor (GR + MR). The loops are activated from the
adrenal gland to the hypothalamus, frontal cortex and hippocampus.
When receiving this information the hippocampus itself also inhibits
the hypothalamus directly (I am not sure how). all this feedback loops
result termination of CRH and in turn ACTH and glucocorticoids
production.
By the way, I think one major out come of a non-working feedback loop
which results accumulation of glucocorticoids is depression.
I have encountered many articles that have conducted inductive expression of transcription factors by cloning their respective inductive promoters -- constitutive expression is detrimental to plant life. Since the stress response also elevates HSPs in plants that ameliorates plants' response against stresses, and what I believe also induces resistance against pathogens, are there articles regarding overexpression of HSPs in plants that have shown to revamp plant performance?
Stress response of microbes can decide whether they will be killed by an antimicrobial agent or they will develop immunity ? Is there a way out to use it for efficient killing of microbes?...suggestions...
Traditional paychosocial measures of race based discrimination stress do not take into account biological factors. I am interested in measuring the effecfs of chronic racial discrimination stress on blood sugar control in diabetic African Americans by incorporating biomarkers of stress response with a questionnaire. What considerations would need to be taken in terms of analysis, choice of questionnaires and appropriate biomarkers for this population?
Thanks for any guidance you can offer.
If anyone knows something about this topic please answer me. Thank you!
In response to stress certain genes are induced. These genes are induced due to some transcription factors (TFs). So how are these particular TFs transcribed in response to that particular stress? Just trying to better understand things. If anyone can give me a good reference, will be thankful.
I am interested in working in Malawi and would like to use measures that have been adapted appropriately. Any articles welcomed.
I want to know the assay for DNA damage in response to pathogen and heat stress and also due to reactive oxygen species produced in stress response
I’ve run into a problem with a research project that I’m completing. Previous research has demonstrated a curvilinear relationship between PTG and stress responses, with the relationship resembling an inverted U-shape (e.g., McCaslin et al., 2009; Taku et al., 2015). The same finding has been found with previous research comparing the relationship of PTG and depression (e.g., Kleim & Ehlers, 2009). I am working with data from a sample of veterans treated for PTSD in a residential setting, and I’m comparing PTG with depression. My analysis also produced a curvilinear relationship, but with a U-shape (not inverted-U). This is very different from previous research. I have noticed that mean scores for depression are much higher than in previous studies (M for BDI-II = about 24- moderate depression). The mean PTGI score is about 55. I’m not sure what to make of this, and am wondering if I should just scrap my project as it is quite different from findings of previous studies. Does anyone have a possible explanation as to why the curvilinear relationship might be so different? Thanks.
Hi all,
So I am treating MCF7 cells with a drug and I have a vehicle (DMSO) control. The DMSO in the control is at 0.022% which is below the limit of 0.1% but I am seeing stress responses in my vehicle control, as much as my treated (via western blotting). I am seeing p53 levels for my control at the same levels as my treated, including LC3B, PARP cleavage. Has anyone experienced this?
Dear all,
I want to test the nitrosative stress response with the fungi.
Can I use sodium nitrite (NaNO2) to be the nitrosative stress?
Thank you all.
I want to establish the high efficient cardiomyocyte differentiation system using mouse embryonic stem cell (mESCs). My colleague told me the hanging drop method (old and classical but famous one). This worked well a little bit but it was not enough efficiency (around 1-5%) to conduct further studies.
I want to research the stress response of mESC-derived cardiomyocyte. It needs more purity and mass. If you know other better methods, please tell me.
Now I'm planning to try the two following methods; glucose-depleted lactate-added culture (Shugo Tohyama, 2012) and Noggin treatment (Yuasa S, 2005).
I am looking for an answer to a rather systemic biology question - is there a 1:1 relationship between HPA axis activation and stress response (in terms of the whole body, I mean not the cellular or tissue stress). I mean could an experimental model without functional HPA axis experience stress in its proper sense of word? I know that it depends on what how we actually define stress, but still, is there any possibility of generalized stress response without HPA axis activation? Will be very grateful for any suggestions, Julie
I am trying to verify if the bulge tester I built is working correctly. The material I am testing is a PP-PE copolymer.
I have carried out 3 bulge tests. These have shown that the Major and Minor strain vary by about 5% to 8% meaning the stress state is very close to biaxial. I also carried 3 uniaxial tensile tests each in both the transverse and longitudinal direction.
The problem I am facing is that theory states that for metals, biaxial equivalent true strain should be double the uniaxial strain at fracture following the same stress-strain curve. My tests show that true strain in biaxial is about double of uniaxial strain at fracture, but the stress-strain curves do not line up correctly. Please see http://i.imgur.com/CeghDRJ.png for the stress strain curves.
Here is the equivalent strain response: http://i.imgur.com/LSfvHV1.png
One thing to note is that the strain response for the biaxial bulge tests is non-linear. I don't think this is the reason for the difference between uniaxial and biaxial stress-strain curves. Since I am straining much faster during bulge testing, I would expect the stress response to be much higher after .15 strain on the stress-strain plot which is not the case.
Is there something special going on in polymers that explains the difference between the two results? I could not find much research papers that show this difference.
STUB1/CHIP is a E3 ligase protein that ubiquitylates and tags other proteins for degradation. It is involved in major developmental processes for the downstream expression and/or stabilization of other proteins. But its function is majorly seen in heat-induced stress response, wherein ER stress is regulated by degradation of mis-folded proteins in the ER, after being tagged by STUB1/CHIP.
In fact, in vivo data for STUB1 knock-down in mice, showed increased sensitivity to heat-induced apoptosis and accelerated aging. Putting all this data together, should CHIP/STUB1 be considered pro-survival or apoptotic?
This is going to be the basis of my future research.
We have generated multiple mutant lines (ATAF family) to see some drought stress response in order to undestand some protein interaction. Could you please advise and suggest me some methods to obtain preliminary data regarding drought stress assay?
Hi friends
I'm writing a user subroutine for a viscoelastic material and material parameters are depend on shear strain rate. I use DSTRAN(3)/DTIME to relate parameters to the shear strain rate in shell element. abaqus solves the problem but during the analysis strain increment becomes zero and parameters and stress response become wrong. Did anyone have such problem with shell elements?
I'm studying the function of a gene whose expression levels are significantly increased in response to different stress-inducing conditions. I would like to assess the presence of stress responsive elements in the promoter. Any suggestion? Thank you.
Hi Everyone,
I am trying to find stress responsive cis elements in the upstream regions of miRNAs in Brassica napus. miRbase gives me the genomic coordinates of miRNA precursor gene, but I am not able to find those in Brassica database.
I am attaching a link for one such miRNA, the coordinates are given in the Genomic Context.
I would highly appreciate if some one could guide me on how to find the upstream region from the coordinates.
Thanks
I am looking for a method to detect stress in response to a fear or pain event. The test will be used to compare the response level of birds to different short term exposure to fear and pain situations.
Can anyone recommend literature on the effect of cell sorting on proliferation capacity / stress response / senescence of the sorted cells (especially MSC)?
How is the cell size influencing the stress response?
Based on Sorge et al. 2014: Experimenter sex can alter behavioral baseline responses (Nature Methods)
