Questions related to Stress Physiology
I am currently researching the stress physiology of photosynthesis. I have previously worked with hydroponic systems using heavy metals at concentrations around tens of microMolars. Now, I would like to extend my research to potted plants and induce cadmium (Cd) stress. However, I understand that I cannot use the same concentration of heavy metal solution in potted plants due to various factors related to the substrate, which might prevent me from observing a significant stress effect. What would be the most appropriate concentration or solution to effectively induce stress in potted plants?
measured blood sugar level after and before electrical stress. I have statically no incidence of stress on the blood glucose values. Do you have more information on the stress response and catecholamines, corticosterol and blood glucose level for broilers?
The research area is not only limited to plant biotechnology, but also extends to plant physiology and biochemistry/Genetic engineering/plant growth and development/secondary metabolism and regulation/stress physiology/Plant molecular biology etc.
your valuable suggestions will be highly appreciated.
We are in the process of developing a multimodel-multisensor wrist band with variety of sensors including Heart monitor, EDA, Accelerometer, body Temperature and others. Please drop a message here if you think that you will be interested in using such device.
When the plant is subjected to abiotic stress, physiological, anatomical and phenotypic changes occur in the plant, as well as free radicals or the so-called reactive oxygen species (ROS) are released.
I started my PhD study about stress and daily activity and I need to find appropriate device for long measurements (+24). I've found two interesting devices so far: E4 wristband produced by Empatica (https://www.empatica.com/e4-wristband) and BioHarness 3 by Zephyr (http://www.zephyranywhere.com/products/bioharness-3). Did you use one of them during your studies? How do you assess their quality? Or maybe did you use something another?
I'm interested in measuring such signals as: EDA, heart rate and motion. Thank you for each proposition of device and your opinion.
In 1st step we have extracted plant pigments in 100 %acetone .
Step 2 ... Acetone extract is mixed with petroleum ether in separotory funnel .
Step 3 ......Acetone containing chlorophyll is washed with slow addition of water (here we assuming that carotenoid pigment comes in petloum ether layer )
4th step .....For separating xanthophyll from other carotenoid pigments we added ethanol .....Gradient of dark green to pale green is formed from top to bottom ......Now we assuming that lower portion is xanthophyll and upper carotenoid...
Does this procedure is correct and satisfactory for quantification of xanthophyll ?????
For the purpose of our project focused on stress measurement, I am looking for a method for calculation of heat production in the human body using simple available variable (e.g. RMR, BMR, body surface area, body composition, weight, height, etc). It has to be a calculation on already measured variables, not a new measurement. Any suggestions how to calculate the heat production as precisely as possible using above mentioned variables will be highly appreciated, thank you! Julie
Dose amylase can be used as a indicator to assessment sleep quality?
I am analyzing some data collected from a sleep study. one of the indices is salivary amylase. i found some papers said consecration of salivary amylase related to sympathetic nerve activity. and consecration of salivary amylase is quite level in daytime but decline during sleep period. so i think if the value of salivary amylase is low before sleep and high after wake up in situation A, but no not changed in situation B. can i explain this result as: situation A provide people a better waking up than situation B?
I'm looking for a valid instrument measuring state stress. My participants will watch a movie and I want to measure how stressful this video was for them (I'm also going to measure cortisol, but I want to add a self-reported stress scale).
Most of the instruments I found so far focus on experienced stress in the last week, months or as a consequence of a task (cohen's, SOS,..)
But I am not interested in the time before the experiment.
Hello to everyone
I am working in the field of stress physiology, I am comparing different parameters between Male animals Vs Female animals and within Male and female animals, each group contains three different time duration of stress. For this, I have to do two-way ANOVA in the SPSS (version 20) software. would you please explain to me how to do two-way for in this case.
I am giving stess to animal during the gestational period with three different trimester first, second and third trimester.
Example 1: for example i am calculating body weight gain during gestational period 1-10 days and 11-22 days gestational period for all stress exposed groups and a control groups.
Example 2 : I am doing Neurobehavioural analysis (anxiety behaviour) for offspring both sex male and female of three stress exposed group and a control group.
how to do two-way ANOVA for these cases and how to enter the data in the datasheet. In these cases which i have to take as dependent variable, independent variable and fixed factor.
Here is the sample data in the data sheet. please help me out
Thanks in advance for your valuable suggestion.
Does anyone either know how long it takes for glucocorticoids type 1 to become saturated in baboons? or how long after an acute stressor the hormone takes to reduce to baseline or non-behavioural change levels? or maybe just how long it takes for them to start decreasing? I understand this may depend on whether the animal is dominant or subordinate depending on deficits in negative feedback loops...
Hello to Everyone,
I am working in stress physiology, since corticosterone is the primary biomarker for the stress response. I want to measure cortiocosterone levels in the pregnant rats. Anesthetizing rat during pregnancy, will it be adverse situation to the developing fetus? will it affect the healthy developing fetus in anyway?
if so, Which would be the suitable anesthesia for this study?
Thanks in advance for your valuable suggestion.
How can you measure stress physiologically? That is not self report with psychometric measures, salivary cortisol or hair.
I know that blood pressure could be a way, but is not as valid since it can be a consequence of other things such as drinking caffeine...
Hi, I want to use the acute social defeat stress but I don't know which strain should I order, the literature says in general only CD1.
In my previous question I asked: "How can we measure the stress Physiologically?" I am really very thankful so many answers and good suggestion we received. Few of them suggested about Salivary alpha amylase. Now I wish to know: How and why we check the Salivary alpha amylase?
I am making a research on athlete to know how stress they are. But i can't find a normal values for salivary alpha amylase.
is there any collection of visual illustration metaphors to characterize the different set of human stresses and to enhance the comprehension of the stress instances in the Human Stress Ontoloty (HSO) ?
Stress is one of the major cause of Eating disorders. During stressed condition Vitamin C and B vitamins deficiency occurs. I want to know if there is any direct relation to vitamin deficiency and Eating disorders?
Many commercial and research technologies advertise the ability to detect stressful states, yet it is unclear which ones are most valid. Has there been any research comparing multiple different measurements (e.g., HRV, GSR, respiration, EEG) to subjective ratings of stress (what participants -actually- report feeling) and also levels of cortisol (blood, saliva, etc.)?
It would be really, really helpful to see a cross comparison to get a better sense of what is the least bogus proxy to stress. Thanks a bunch!
I used Open field plus sucrose preferential. However, I receive this question when I'm presenting my stress-depression animal model. One of the questioner, a professor ask me this as he is using a substance that greatly affected the mouse prefrontal cortex and the mouse barely move.
I'm interested to measure cumulative stress effects of fish life-history strategies. Thereby I'd like to observe stress levels during the development as an individualistic time series. Main problem is that catching, anaesthesia and blood sampling may cause extra stress to fish and bias to data. Does anyone have any experience if the stress could be measured any other way more reliable? Or is there any reliable indicator for stress than stress hormones that could be measured?
The research is on the impact of stress on job performance as it relates to employees in the school (teachers, administrators, auxiliary staff).
it is said that the most Knee OA is age related: wear and tear due to daily joint use causes some damage to the knee joint which is not followed by the repair process. Sp
what is the physiological pathway which is protecting the knee joint in normal people? and what is the pathophysiological pathway which leads to damage of cartilage in the knee Joint in knee OA?
I was analysing the literature on stress levels of different professional categories. Unfortunately, I found that the category I am most interested in are sampled for salivary cortisol; the other, which I hoped to use as a benchmark, are sampled for blood or urine cortisol. Is there any way of making findings with these different techniques comparable with each other?
Thank you in advance
I want a scale to measure the current state of stress of a subject in an experiment in which, i will differentiate the level of stress before and after the activity (duration one hour) of the the subject. kindly help me. thanks.
I am looking for an answer to a rather systemic biology question - is there a 1:1 relationship between HPA axis activation and stress response (in terms of the whole body, I mean not the cellular or tissue stress). I mean could an experimental model without functional HPA axis experience stress in its proper sense of word? I know that it depends on what how we actually define stress, but still, is there any possibility of generalized stress response without HPA axis activation? Will be very grateful for any suggestions, Julie
I am planning to assess stress level in HIV infected couples but get confused as many scores are available. Which is the best score for Indian subcontinent?
in the case of broilers, to prove the state of animals stress, i don't know if i should mesurate the cortisol or corticosterone levels?
We are in the planning phases of a sleep and memory consolidation study using a napping paradigm. We are a bit concerned about the differences in cortisol levels for early morning naps (i.e. 9:30-10:30am) relative to naps in the afternoon (i.e. 3:30 to 4:30pm).
I am aware that naps can reduce cortisol levels, but I am having trouble locating a paper that describes absolute levels of cortisol in morning vs. evening naps.
My hunch at the moment is that morning naps do have more cortisol as they occur closer in time to the cortisol awakening response associated with the preceding night of sleep, but a definitive answer on this issue would be great (bonus points for pointing me to a publication!). Thanks for your time.
Hello to everyone!!!
I am working in the field of stress physiology, my work is about i will stress to mother during pregnancy and will check cognitive functions of its offspring.. I will using 40 days old pups for further experimental studies,, will it differ maternal stress effect and cognitive functions of the offspring before and after puberty period of the animal...
Muscle pain, shortness of breath, and digestive problems are symptoms of anxiety and depression that can be attributed in part to chronic muscle tension. I was wondering if anyone has information regarding studies measuring range of motion in depression and/or anxious people.
Can someone explain differential screening of subtractive cDNA library with illustration?
I want to spray Kaolin on hazelnut trees exposed to drought stress in order to decline negative effects of stress on physiological aspects. I knew that kaolin uses for decrease the negative effects of sunlight. The orchard where hazelnut trees are cultured is a sunny place with approximately 30-35 °C temperature (in summer).
Do you think it (kaolin) can be useful for decreasing the negative influences of drought stress on hazelnut trees?
I saw a poster at a conference that presented data suggesting that by altering the pH and/or the volume of 4% PFA during transcardial perfusion it is possible to tailor the fix for optimal labeling with individual antibodies. Does anyone have any experience with this?
An ABA-insensitive arabidopsis mutant (screened by myself) is that which can produce green cotyledons on MS supplemented with 3, 5 and 10 uM ABA.
4-week old soil grown arabidopsis plants were brought under abiotic stress (salt and drought) for 2 weeks. Less accumulation of MDA and H2O2 was observed in mutants as compared to wild type. What would be the possible reason and mechanism for less accumulation of MDA and H2O2 under stresses?
GSR has been used to determine stress levels, cognitive dilemma and response inhibition during tasks. My subjects completed brief surveys and a belief questionnaire while skin conductance levels were collected every .05 seconds. With my software, I am only given the GSR levels and timing as well as a simple visual line graph.
I would like to know how to measure overall fluctuation during the task. While the graph shows me "significant differences". Averages and standard deviations are "insignificant." I'm assuming there is a definite way to measure overall fluctuation in the entire process and would greatly appreciate some advice and guidance.
I am searching for different adoptive physiological and anatomical changes in halophytes which enable them to tolerate salinity.
We are generally monitoring the stress conditions by hormones and it is very interesting to see the physiology of stress.
We are making "open field", but as I know it is for emotional estimation of animals and do someone know the connection with stress?
Also, what kind of tests are more useful?
We are investigating the cortisol concentration before and after a stress-inducing task (Cyberball) and we’d like to know if there’s a problem if subjects have a dry mouth after the task (which we experience quite a few time with our subjects) or if the salivary flow is increased because of the task. We received a suggestion from a supposed expert, that the contribution of cortisol to saliva is continuous which may be a problem if there’s a different amount of saliva produced but the hormone contribution stays the same. This would mean that the concentration of cortisol is primarily influenced by the salivary flow and not by the actual release of stress induced cortisol.
Is this actually the case or not???
What we would need in this case is some kind of reference-values that can tell us how much saliva has been produced in a certain time.
Does anybody know if there is this salivary influence and do you have any suggestions for a reference or how to handle this problem?!
And does anybody know the influence of salivary flow on testosterone, too?
All suggestions are welcome!!! And in advance - thanks a lot for all answers!
I plan to investigate whether the salivary cortisol levels can explain the clinical responses in patients with low back pain (LBP) following a conservative treatment. Specifically, I want to see if patients with higher stress level (higher salivary cortisol concentrations) will have poorer post-treatment clinical outcomes. I initially plan to collect a saliva sample from each participant at 30 minutes after waking. However, an expert suggested me to take at least 3 samples from each participant because of the high variability in intra-individual salivary cortisol concentrations. Apart from the variability, I find that salivary cortisol concentrations may be affected by depression, age, menstrual cycle, oral contraceptives or menopause. It implies that I need to adjust for many factors before I can meaningfully interpret the relation between salivary cortisol levels and LBP outcomes. In summary, I may need to either exclude participants taking oral contraceptives/reaching menopause or adjust for all confounders in my 32 participants. However, if I want to put all the confounders into the final regression model to explain the relation between LBP outcomes and cortisol, I need more participants. Any suggestions? Have I missed any other potential confounders?
I am investigating the stress of capture and confinement on wild animals. Plasma aptoglobin levels are an indicator of chronic stress and handling procedures in cattle and pigs. This new ELISA is supposed to be deer specific, but is rather expensive so I need affirmation that it really works.