Staining - Science method

Background The purpose of this scoping review is to understand the etiological, clinical characteristics and treatment of chromogenic staining of teeth and the various management strategies reported in literature. This SR was performed in accordance with the PRISMA 2022 guidelines and was registered in the PROSPERO database (CRD42024565446). Metho...

Elastofibromatous changes of the oral mucosa, such as an elastofibroma (EF) or an elastofibromatous lesion (EFL), are not well recognized, and the second such case in Japan is reported. A 72-year-old man wearing a complete maxillary denture presented with a small nodule on the hard palate. Histopathological examination showed abundant fibrous tissu...

We propose an imaging system and methodology for mapping soft-tissue samples in three dimensions, with micron-scale and isotropic spatial resolution, with low-concentrations as well as in the absence of heavy metal staining. We used hard x-ray phase-contrast imaging for the x-ray ability to nondestructively probe the internal structure of opaque sp...

Metallic sandwich panels featuring cellular metal cores are widely utilized across various sectors due to their exceptional load‐bearing efficiency and design versatility. However, their application is often limited by the challenge of shaping complex geometries. This study introduces a novel thin sandwich panel incorporating stainless steel wire m...

Background As in numerous cancers, the connection between the Ki-67 proliferation index and response to treatment in cellular breakdown in the lungs is underlined. The purpose of this study was to investigate the connection between the Ki-67 proliferation index and radiotherapy’s therapeutic and survival effects in small cell lung cancer. Methods...

Form-function relationships often have tradeoffs: if a material is tough, it is often inflexible, and vice versa. This is particularly relevant for the elephant trunk, where the skin should be protective yet elastic. To investigate how this is achieved, we used classical histochemical staining and second harmonic generation microscopy to describe t...

Objective: To establish the frost damage to the stem and root nodules and protection degree of products with antifreeze potential, during the V2, V3, R6, and R7 phenological stages of bean. Design/Methodology/Approach: Black beans with a type II indeterminate bushy habit were collected. Antifreeze (An), amino acids (Am), gibberellins (Gib) and thei...

Background By simultaneously staining multiple immunomarkers on a single tissue section, multiplexed immunohistochemistry (mIHC) enhances the amount of information that can be observed in a single tissue section and thus can be a powerful tool to visualise cellular interactions directly in the tumour microenvironment. Performing mIHC remains techni...

Subcellular Spatial Transcriptomics (SST) represents an innovative technology enabling researchers to investigate gene expression at the subcellular level within tissues. To comprehend the spatial architecture of a given tissue, cell segmentation plays a crucial role in attributing the measured transcripts to individual cells. However, existing cel...

Objectives To address the lack of nerve and blood supply after labial salivary gland transplantation (LSGT) resulting in glandular atrophy. We designed a modified LSGT, called insular infraorbital neurovascular pedicle LSGT, and evaluated the postoperative efficacy. Design This is a prospective, single-centre, self-contained study. Setting The re...

Background The most frequent type of non‐Hodgkin lymphoma (NHL) is diffuse large B‐cell lymphoma (DLBCL). Although lymph nodes are the most commonly affected organs compromising 70% of DLBCLs, only 5% of extranodal lymphomas represent skeletal muscle involvement. Specifically, abdominal wall muscle involvement is rare and there are only a few repor...

In nature, filamentous fungi interact with plants. These fungi are characterized by rapid growth in numerous substrates and under minimal nutrient requirements. Investigating the interaction of these fungi with their plant hosts under controlled conditions is of importance for many researchers aiming to proceed with molecular or microscopical inves...

Background Overexpression of tear matrix metalloproteinases-9 (MMP-9) on the ocular surface tissues has been reported to result in ocular surface damage. MMP-9 levels in tears have been listed as one of many tools for confirming dry eye disease (DED). Objective This investigation aimed to compare MMP-9 levels and ocular surface parameters in diabe...

With the advancement of computer technology, dynamic detection of damage to tire in-service has become feasible. However, current methods often struggle with accuracy limitations when confronted with specific working conditions and external factors. To address this challenge, we propose an intelligent recognition system for in-service tire damage d...

The cost implication of high iron (III) content in borehole water for domestic use is a common problem globally. Iron (III) is the major cause of the reddish colour in water which gives bad sight as it stains bright surfaces, clothes etc., in homes and other places. For this reason, several methods to reduce it have been proposed. This work employe...

Background Pancreatic cancer (PC) is a highly aggressive malignancy with a poor prognosis due to its late-stage diagnosis and limited treatment options. Objectives This study aimed to elucidate the molecular mechanisms underlying PC progression and identify potential molecular targets for its diagnosis and treatment. Methods DAZAP1 expression in...

The micronucleus test is one of the most popular genotoxicity assays. In order to avoid underestimation of micronuclei frequencies by counting non-replicating cells, the cytokinesis-blocked micronucleus test has been developed. In this technique, only binucleated cells are scored. One underestimated problem is the potential difficulty in discrimina...

Bloodstain Pattern Analysis (BPA) helps us understand how bloodstains form, with a focus on their size, shape, and distribution. This aids in crime scene reconstruction and provides insight into victim positions and crime investigation. One challenge in BPA is distinguishing between different types of bloodstains, such as those from firearms, impac...

Objective Rapid on-site evaluation (ROSE) of respiratory cytology specimens is a critical technique for accurate and timely diagnosis of lung cancer. However, in China, limited familiarity with the Diff-Quik staining method and a shortage of trained cytopathologists hamper utilization of ROSE. Therefore, developing an improved deep learning model t...

This study explores an advanced approach to automatic breast cancer characterization by leveraging convolutional neural networks (CNNs) trained on 224x224 pixel patches from whole-slide images (WSIs). I compared two architectures, VGG-16 and WRN-4-2, across two classification problems: benign versus cancer, and benign, DCIS, versus IDC. Results ind...

Background/Aim Karyopherin alpha 2 (KPNA2) has been reported to be associated with cancer aggressiveness and treatment resistance via transporting several cargo proteins into the nucleus, such as cancer-promoting E2F and DNA repair-related MRN complex. Recent studies have highlighted the KPNA2 functions in tumorigenesis and the progression of vario...

This report describes an atypical case of canine acanthomatous ameloblastoma (CAA) with mucinous glandular differentiation in a 12-year-old male Chihuahua with a right mandibular mass. Initially diagnosed as CAA by biopsy, computed tomography revealed bone lysis, prompting surgical excision. Histological examination showed lobular growth with two d...

The aim of this research is to determine the calculation of the cost of production costs incurred, the accounting treatment of damaged products and the results of analysis after calculating damaged products. The research was conducted at CV ABC Bekasi. The research uses quantitative research methods with a qualitative descriptive method approach. T...

English language has become unavoidable being a global language in the present era. Whether it is a field of business, technology or education, its significance can’t be denied. Thus, majority of the world is learning and speaking English owing to its dire need. Unfortunately, despite staining through different reforms, the orthography of English l...

Enamel microcracks are often apparent in the teeth of elderly individuals. However, accurate clinical diagnosis of enamel microcracks is very difficult. The purpose of this study was to examine the utility of inspections for enamel microcracks using a near-infrared light transillumination device with fluorescence staining. Human upper incisors with...

The endoplasmic reticulum and the internal nuclear compartments are intrinsically connected through the nuclear membrane, pores and lamina. High resolution imaging of each of these cellular features concurrently remains a significant challenge. To that end we have developed a new molecular nuclear membrane-endoplasmic reticulum (NM-ER) staining flu...

Background: Hepatocellular carcinoma (HCC) is one of the deadliest types of tumors. MicroRNA (miRNA) MTCO3P38 is a novel miRNA derived from the pseudogene MTCO3P38 with 18 nucleotides in length. Methods: The target genes of miR-MTCO3P38 were predicted by Targetscan, RNAhybrid and PITA. Transwell assays verified the effects of miR-MTCO3P38 and its t...

A man in his 70s was referred to our department for the first time with a complaint of staining of his underwear for the past 3 months. He was diagnosed with anal fistula (type IILS). Preoperative MRI revealed mucus accumulation in the fistula duct on T2-weighted images, and it was determined that hemorrhoidal anal fistula cancer could not be ruled...

Breast cancer is the most prevalent form of the disease and the primary cause of cancer-related deaths among women globally. Early detection plays a pivotal role in substantially diminishing both the morbidity and mortality rates associated with this disease in women. Consequently, the development of an automated diagnostic system holds promise in...

We investigated risk factors for neonatal morbidity based on a database of 19,268 singleton maternal deliveries atPattani Hospital during the period from 1 October 1996 to 30 September 2005 inclusive. This database includesdemographics of the mother and delivery outcomes including birth weight, one- and five-minute Apgar scores, and atmost one comp...

Background: The current study was aimed to detect and localized of human endometrium stem cells at different age, through using CD105 and CD90 as stem cell markers. Materials and methods: Fifty specimens of female adult endometrium from different age groups, including 20, 30, 40, 50, and 60, years were prepared for histological technique. Samples o...

SYBR Cell staining in MICO lab

Dry Eye Disease (DED) is a prevalent ophthalmological condition characterized by tear film instability and ocular surface damage, leading to eye discomfort, visual disturbances, and inflammation. Objective: To analyze the age-dependent response to topical lubrication in patients with DED over a period from May 2023 to October 2023 in a cohort of 90...

Hepatic hemosiderosis is a common nutritional disease affecting numerous bird species. The aim of this study was to validate the use of pinch hepatic biopsies to quantify the hepatic iron in Asian glossy starlings (AGS) and compare clinical measures usable for antemortem disease monitoring. Following euthanasia, serum, liver pinch biopsies, and who...

Background/objectives: The MTHFR677C>T gene variant results in a thermolabile MTHFR enzyme associated with elevated plasma homocysteine in TT individuals. Health risks associated with the TT genotype may be modified by dietary and supplemental folate intake. Supplementation with methyltetrahydrofolate (methylTHF) may be preferable to folic acid be...

The importance of collagen and elastin remains incompletely understood concerning tumor immunity in cancer tissues. This study explored the clinical significance of collagen and elastin deposition on tumor immunity in advanced colorectal cancer patients. The collagen and elastin contents were assessed simultaneously using elastic van Gieson (EVG) h...

Background The involvement of microRNA-668 (miR-668) in the onset and progression of renal fibrosis remains unclear. To this end, we aimed to explore the relevant mechanism of miR-668 in renal fibrosis. Methods C57BL/6 J male mice were randomly divided into sham-operated, unilateral ureteral obstruction (UUO), and UUO-fenofibrate groups. Based on...

To address the challenges of low fixation and poor resistance to staining on white ground of reactive dyes in the dyeing and printing industry, two new polyfunctional reactive dyes (MFA-1 and MFA-2) were developed. These dyes, which contained m-phenylenediamine derivatives with sulfonic and methyl groups as bridge groups, demonstrated high solubili...

Background: The presence of meconium in amniotic fluid is indicated one of the foetal distress sign. The objective of the study was to see the association of meconium stained liquor and Apgar score of neonate. Methods: Women admitted in labour room were classified as those with clear liquor or meconium stained. Labour was monitored and Apgar score...

Introduction Small cell lung cancer (SCLC) is known for its high proliferative rate and poor prognosis. Although Delta‐like ligand 3 (DLL3) is specifically expressed on the surface of SCLC, the association of DLL3 with prognosis in SCLC remains uncertain. Hence, we aimed to evaluate prognostic role of DLL3 in extensive stage of SCLC treated with fi...

Geological assessment of rock lithologies of the fourteen deer stones at the Uushigiin Uvur deer stone complex, west of Mürün in north-western Mongolia, indicated that natural jointing a product o regional geological stresses in the insitu rock-was an important component in stone selection by the deer stone fabricators. Near surface exfoliation fra...

Maravalia echinulata (Niessl ex Rabenh.) Ono has been rediscovered from West Bengal, India, after its last report in 1931. Sections of infected host leaves were cut, stained in cotton blue, and mounted in lactophenol. All five spore forms of this macrocyclic autoecious rust fungus are described in detail, with notes on its world distribution, distr...

This study assesses a locally formulated Liquid-Based Cytology (LBC) transport medium in comparison to conventional cytology, aiming to enhance cellular preservation and cytological accuracy. The formulation process involved careful selection of reagents based on their compatibility with cytological applications, yielding a unique medium tailored t...

Germination test for papaya seeds demands thirty days to obtain the results, which is a long period for decision-making. Thus, rapid tests become essential tools to evaluate seed viability, and the tetrazolium test is an alternative for this purpose. Hence, this study aimed to establish the adequate procedure to apply this test, in order to reduce...

Background Urothelial carcinoma (UC) has been considered the most frequent bladder tumor. Although the degree of bladder wall invasion and grade of the tumor are the essential prognostic factors in bladder carcinoma, there is no dependable parameter predicting patients’ outcomes. Objectives To assess the frequency of CD10 expression (in tumor cell...

Microbial contamination of drinking water poses a health threat for man and animals. The aim of this study was to detect Escherichia coli (E. Coli) and Salmonella in drinking water from the Sher-e-Bangla Agricultural University (SrAU) campus and its surrounding areas between January 2021 and August 2021. A total of 60 water samples were collected f...

The Postmasburg manganese ore field currently stands as the second largest in the world, featuring a widespread distribution of mining areas. Remote sensing technology possesses distinct advantages in interpreting geological information at a macro level. To further expand the scope of the Postmasburg manganese ore field and identify new target mini...

The purposes of this research were (1) to design at least 5 styles of straw rope furniture by macramé techniques for housing decorations; (2) to evaluate the straw rope furniture prototypes produced by macramé techniques based on the community products standard criteria. Development stages were employed as the research processes; which are developm...

The purpose of this study was to explore the inhibitory effect of andrographolide on the expression of key regulatory genes involved in the biofilm formation of Staphylococcus epidermidis (SE). Taking the film-producing strain Staphylococcus epidermidis SE1457 as the research object, the effect of andrographolide on the formation of Staphylococcus...

Whole slide imaging (WSI) provides tissue visualization at the cellular level, thereby enhancing the effectiveness of computer-aided diagnostic systems. High-precision autofocusing methods are essential for ensuring the quality of WSI. However, the accuracy of existing autofocusing techniques can be notably affected by variations in staining and sa...

Introduction: Action bitters, commonly used in traditional medicine, have an unclear safety profile and potential effects on lung, liver, and kidney tissues. This experimental study investigated the histological effects of action bitters on the lungs, liver, and kidney tissues of adult male Wistar rats. Understanding these effects is crucial, as it...

Background Ameloblastoma is a benign odontogenic neoplasm with a high recurrence rate. Identifying cellular and molecular changes in this neoplasm may help predict the recurrence risk. Bcl-2 and galectin-3 are anti-apoptotic proteins associated with the prognosis of many neoplasms. However, there are a few studies focusing on the association betwee...

The aim of the present work is to study the atmospheric corrosion behavior of metals exposed to both urban (Milan, IT-Lombardia) and marine (Bonassola, IT-Liguria) atmospheres in Italy. A number of coupons (100 × 150 mm) of carbon steel (CS), hot-dip galvanized steel (GS) and different grades of stainless steel (SS) were exposed. At fixed periods o...

This study introduces a federated learning-based approach to predict HER2 status from hematoxylin and eosin (HE)-stained whole slide images (WSIs), reducing costs and speeding up treatment decisions. To address label imbalance and feature representation challenges in multisite datasets, a point transformer is proposed, incorporating dynamic label d...

Clear cell renal cell carcinoma (ccRCC) is a highly lethal urinary malignancy with poor overall survival (OS) rates. Integrating computer vision and machine learning in pathomics analysis offers potential for enhancing classification, prognosis, and treatment strategies for ccRCC. This study aims to create a pathomics model to predict OS in ccRCC p...

Objectives: to evaluate the risk of malignant potential through toluidine blue staining and dysplastic grading of verrucous papillary lesions of the oral cavity (VPLO). Materials and methods: patients with VPLO located in the oral cavity were identified to whom toluidine blue was applied and dysplastic changes were subsequently evaluated and grade...

Pronunciation plays a vital role in effective communication, ensuring that a speaker's message is clearly conveyed to the listener. This research explores the challenges faced by EFL students in pronouncing assimilation in English connected speech, particularly focusing on difficulties with various types of assimilation, including /t/, /d/, /n/, an...

Soil hydraulic properties are mainly governed by the soil’s heterogeneity, anisotropy, and discontinuous structural characteristics, primarily when connected soil macropores characterize the structures. Therefore, researchers must document reliable hydrological models to elucidate how the soil medium affects the movement of soil water. This study,...