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Is there any protocol for staing thick speciments?
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Hello Kartarzyna, it depends on the kind of your tissue. If you are work with compact tissue like brain, liver, kidney, spleen you can treat 20 to 50µm wtih a immunhistochmical protocol. Even here it depends on the protein you like to detect. The free floating method is very commen in neuroscience.
Here a short protocol:
use microtiter plates with 24 wells; each row of the plate is one working step.
1. Rinsing 3 x 10 min in PBS/TBS
2.Blockin of endogenous peroxidase (ony with Peroxidase containing detection systems).
3. Rinsing 3 x 10 min in PBS/TBS
4. Blocking of unspecific antibody binding (with normal serum or BSA) -- 90 min
5. Primary antibody (dilution has to be testet at first) -- overnight
6. Rinsing 3 x10 min PBS/TBS
7. secondary antibody biotiniylated or with Peroxidase or with fluorophores labeled -- 90 min
8.Rinsing 3 x10 min PBS/TBS
9.. StreptAvidin peroxidase or Fluorophore labeled -- 90 min
10. Rinsing 3 x10 min PBS/TBS
12. DAB-Staining for brightfield analysis 5-10 min
13. Rinsing 3 x10 min PBS/TBS
14. mounting on gelatine coated slides
13 dehydrating, clearing and mounting in a xylene soluble mounting medium (DAB)
or mounting with a water solubel mounting medium with or without Dapi for immunfluorescence
your dilution medium should contain 02.% Triton-X 100 and 1 % normal serum
The reaction should be processed on a rocking-table or shaker at room
temperatur. You can put your plate in the fridge but depending on the antibodies you will need higher concentrations.
Every antibody concentration has to be tested before use.
Good luck!
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Does anyone know how to solve AbaqusExecutionError('Abaqus/Explicit Packager', -1073741511) , there are no any .sta or .msg files generated .for, .inp and .dat files are fine. [*1] I can easily run all subroutines listed in https://lnkd.in/dMqZNyCm [*2] Abaqus verification shows pass on user subroutine with standard and explicit. Abaqus2020 vs2022 inteloneapi 2023 [*3]
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Intel 2017 compiler with visual studio 2015 and Abaqus 2019, solved the issue. Now the subroutine runs.
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Hi everyone
I have a tooling simulation .sta file format which looks like it is Abaqus file format. I don't know with which software it has been made but it contains object's mesh coordinates.
Now I want to use it with ANSYS and even conevrt it to more conventional formats like STEP.
Can anyone help me in this regard?
Thanks
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You can import/open it in Altair Hyperworks.
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I am using NetSim 802.11 n / ac library and wanted to know how to vary CW Min and CW Max. More specifically, how do I
make (i) a global CW change that affects all APs/STAs (ii) a local CW change that affects only some APs/STAs
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I want to stain some bouine fixed paraffin embedded mouse brains from more than 30 years ago for Nissl (Cresyl Violet) staining, but should remove Picric Acid first (there was no staining at all). I found in re-hydration steps, I should use 70% EtOH with Lithium Carbonate (saturated), does anyone know how much lithium carbonate should I add to how much 70% EtOH? And for how long I should keep my tissue in 70% EtOH+Lithium Carbonate?
Any other effective methods for removing Picric Acid?
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Thank you Daniel for your advise.
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I would like to know if there is any seismological processing of local data by python. (MSEED format)
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Thanks,
For while, I am looking for STA/LTA program, but I am doing one and I am using STA/LTA obpsy tutorial.
I am using the recursive_sta_lta (obspy.signal.trigger) and attributed a cft as the tutorial. After the index will be the sampling_rate time to cut the signal for analysis.
When I finish the simple code I will make it available.
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The black curve corresponds to the DTA analysis and the blue curve for the TG analysis. Regarding my previous calculations and the melting temperature of the elements of this alloy, I can guess that the peak available at a temperature of 1080 degree Celsius is the melting point of the high entropy alloy. Is there any person who can guide me about the present peak in the temperature range of 600-800 degree Celsius ?Which analysis, references and papers can i use to more information?
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Sehr geehrte Frau Mamnooni,
Die Legierung kann bei dieser Temperatur oxidiert werden, weil die Masse während dieser Temperaturspanne auch steigt.
Besten Grüßen
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I am working on numerical simulation of pressure wave on metal target. the pressure wave duration is 120 ns, and i am giving simulation for 1 sec. simulation run normal till 1 e-5 sec, then it aborted due to error:
forrtl: The pipe has been ended.
forrtl: severe (38): error during write, unit -1, file CONOUT$
forrtl: severe (38): error during write, unit -1, file CONOUT$
forrtl: The pipe has been ended.
forrtl: severe (38): error during write, unit -1, file CONOUT$
---- error analysis -----
25-04-2021 03:06:53
Begin State File Translator
25-04-2021 03:06:53
Run state.exe
25-04-2021 03:06:56
End State File Translator
Begin Selected Results Merger
25-04-2021 03:06:56
Run mergeSel.exe
25-04-2021 03:06:57
End Selected Results Merger
Abaqus Error: The executable explicit.exe
aborted with system error code 3.
Please check the .dat, .msg, and .sta files for error messages if the files
exist. If there are no error messages and you cannot resolve the problem,
please run the command "abaqus job=support information=support" to report and
save your system information. Use the same command to run Abaqus that you
used when the problem occurred. Please contact your local Abaqus support
office and send them the input file, the file support.log which you just
created, the executable name, and the error code.
Abaqus/Analysis exited with errors
when i check ( .dat, .msg, and .sta) these files
i didn't find any information...
please let me know how to solve this issue
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Seyed Morteza Salimi Thank you very much for your support, i am able to identify the error part.
The solution is:
While defining a job, if we prefer multiple processor for parallel computing, we have to change multiprocessing mode from default to threads.
Thank you very much
i have one more issue, if possible help me
I am working on numerical simulation of pressure wave on metal target. the pressure wave duration is 120 ns. simulation ran for 1E-5 Sec, i was trying to analyze residual stresses, but the stress value i got different for each frame (Every time increment), i thing its due to inertia effect, can you help me to understand its physics?
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I was working on thermogravimetric analysis for some materials, however, because of the pandmy I stoped using the STA TG DTA-DSC 1600 for like 6 months, when I was able to work again with it, the temperature measurement system seems not to be working, now when I connect the software with the analyzer the frunance sensor marks 1740°C all the time, even without heating and the external sensor marks 0°C. Can somebody tell me what the problem might be or any sugestion on how solving it?
Thanks in advance.
BTW: The software I'm using is Calisto v. 1.36.
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The problem was solved, the temperature shown as 1740°C means the thermocpule either is broken or unplugged, this causes the furnance to get locked being unable to lift it, this may happen if one of the wires conected to te furnance breaks, normally because of the movements (up and down) the furnance has to do when getting ready an experiment, any copper wire may work for soldering this part, you can notice which wire is broken by opening the furnance case and looking at the wires behind the furnance, there is a plug that holds like 5 cables, by moving the furnance up and down and having the tga turned on in stand by you can notice that the temperature change when you see that stop the movment and tight them to se if the temperature changes, then you will know which wire you need to fix.
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The executable standard.exe aborted with system error code 1073740791. Please check the .dat, .msg, and .sta files for error messages if the files exist. If there are no error messages and you cannot resolve the problem, please run the command "abaqus job=support information=support" to report and save your system information. Use the same command to run Abaqus that you used when the problem occurred. Please contact your local Abaqus support office and send them the input file, the file support.log which you just created, the executable name, and the error code.
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Mayank Agrawal rename the mkl_avx2.dll in (C:\SIMULIA\Abaqus\6.13-x\code\bin) to mkl_avx2.dll.11.0.0.1 to resolve.
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I am trying to specify 'general contact' in abaqus/standard while modelling honeycomb structures to prevent the honeycombs from penetrating one another while deforming and abaqus crashes with the error message The executable standard.exe aborted with system error code 1073741819. Please check the .dat, .msg, and .sta files for error messages if the files exist. If there are no error messages and you cannot resolve the problem, please run the command "abaqus job=support information=support" to report and save your system information. Use the same command to run Abaqus that you used when the problem occurred. Please contact your local Abaqus support office and send them the input file, the file support.log which you just created, the executable name, and the error code.
Job 30_11_Trial3 aborted due to errors."  I am not using any UEL or UMAT.   Please find the image of model attached.  Thanks  a lot.
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I have same error in my analysis. It starts running but after 2 hours later shows this errror. First I defined the 'general contact' between the brick infill and the frame. I had results then I checked the contact properties additional contact for bed joint behavior was deleted by itself. I think the error apperars because of that. Do you have any idea how I can solve this error or am I doing something wrong?
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Hello everyone,
I'm doing a sheet metal forming simulation on Abaqus and it's my first time working on this software. After submitting the job on Abaqus several times via command prompt and CAE, the job is never completed. In the .sta file it just stops running and it doesn't show errors. The maximum ODB Field Frame Number of all my submitting attempts was 11 of 20 requested intervals, which is not enough.
Does anyone know what could be the reason? I'd be very thankful for any advice.
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Hi Seif,
I don't have much experience of Abaqus/Explicit. However, I've done a calculation using the information above. There seems to be 55151 increments between increments 2302740 and 2357891. It looks like the period analysed was 0.0099 sec, so each increment was 1.795(-7) sec. However, the file shows that the stable increment at element 68 was 1.648(-7). Can you try the following option:
*DYNAMIC, EXPLICIT, ELEMENT BY ELEMENT, SCALE FACTOR=0.9
Regards,
Simon
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The question is what remains in the urban planning and in our professional and intellectual experiences of Giuseppe Campos Venuti and Federico Oliva? What about discipline now?
They reasoned and worked together, they proposed urban plans and organized technical offices, and now they are gone, but their legacy lies in the contributions they have spread throughout plans, conferences, magazines and books.
Campos Venuti and Oliva worked on planning, believing in social services, ecology, infrastructures as tools to build towns without suburbs, towns made up of centralities. Those who, therefore, have found in their words and actions the stimulus and strength to operate as planners and to engage in politics with the conviction of being able to make a contribution to freedom, equality and brotherhood in the territory, are the witnesses aware of their thinking.
La domanda è cosa resta nell'urbanistica e nelle nostre esperienze professionali e intellettuali di Giuseppe Campos Venuti e Federico Oliva? E la disciplina adesso?
Hanno ragionato e lavorato insieme, hanno proposto piani urbanistici e organizzato uffici tecnici, e ora non ci sono più, ma la loro eredità sta nei contributi che hanno diffuso attraverso piani, conferenze, riviste e libri.
Campos Venuti e Oliva hanno lavorato sulla pianificazione, credendo nei servizi sociali, nell'ecologia, nelle infrastrutture come strumenti per costruire città senza periferia, città fatte di centralità. Coloro che, dunque, hanno trovato nelle loro parole e nelle loro azioni lo stimolo e la forza per operare come urbanisti e per impegnarsi in politica con la convinzione di poter dare un contributo alla libertà, all'uguaglianza e alla fratellanza sul territorio, sono i testimoni consapevoli del loro pensiero.
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In the Spanish case, the influence of Campos Venuti (and his Urbanistica e austerità) was huge in the Spanish municipal plans of the 1980s, undertaken by the first democratic city councils for cities that had to correct enormous deficits of public facilities, infrastructure, urbanisation or housing, inherited from the dictatorship. In the 1985 Madrid Plan he was directly involved. In other cases, like Valencia, Málaga or Santiago de Compostela, his ideas had a strong influence.
A book on this Plan of Madrid was recently published. Three articles, contained in this book, written by Joan Roger Goncé, Beatriz Fernández Águeda and Sonia De Gregorio Hurtado clarify several aspects about the relationship between Campos Venuti and Spain.
Otherwise, Federico Oliva colaborated for many years in the journal Ciudad y Territorio of the Spanish Ministry of Public Works and Urbanism.
The relation between Italian and Spanish urbanism was very intense and fruitful since those years. Proof of this are the different tributes celebrated in the Club de Debates Urbanos de Madrid to figures like Francesco Indovina or Bernardo Secchi.
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Hi,
I am trying to use the ABAQUS/standard solver to simulate the single crystal deformation behaviour with DAMASK framework. The materials is BCC ferrite with hooke elasticity and phenopowerlaw plasticity. The model is a 2D cube with a mesh element type CPE8. Boundary condition: left U1 constrained,, bottom U2 constrained, and top with minus V2.
The job ran without errors, but there are many 'position not found when parsing line' warning in the .dat file and 'THE SYSTEM MATRIX HAS xxx NEGATIVE EIGENVALUES. ' in the .msg files. What is the question?
I am new to DAMASK and have insufficient experience. Any suggestion would be greatly appreciated.
Attached are the INP, MSG, DAT and STA files.
Thanks.
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Hi,
Have you solved this problem?
Could you tell me what cause this problem ?
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Hello there,
We have found CO2-Peaks on different aged clay ceramics by STA/DTA-FTIR analysis at approx. 300-400°C and 500-600°C. We found no Carbonates with the XRD. Does anyone have an idea here?
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We dont find any MgO/CaO in the XRD. Maybe it is thinkable that recarbonization takes place in aged / hydroxylated clay ceramics?
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I’m trying to evaluate the thermal behavior of geopolymers in the range of 25 – 1000 °C by means of a LINSEIS STA PT 1600 - Simultaneous Thermal Analysis (TGA+DSC), which use the default software (Tawin L81PT type S). However, the data evaluation module provides the peaks areas values in µVs/mg. Is there any way to convert these values in mW/mg?
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I have a a simulation using mininet-wifi where I want to measure loss, delay and bandwidth of the stations at different distances and received transmission power from associated APs but unfortunately the powers of the AP received to the STAs do not change and I dont know how do I get loss and delays
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The attached document could be of use to you.
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Dear community,
I have a simple (but quite heavy) 3D Abaqus/Standard Model implemented with a UMAT that stops converging when 80% of the step is completed (please refer to the .sta and .msg files). The model loads a short beam in three point bending. I have tried both Newton-Rapson and Quasi-Newton methods with faster performance in the latter. Would you kindly have any suggestion to improve the convergence performance?
Thank you in advance for your help
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you can reduce the minimum allowable time increment less than 1e-6 as stated in Model.sta file. Also you can impose a displacement load in the BC instead of applying the direct load.
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Dear all,
We want to model a pressure vessel using WCM (Wound Composite Modeler). We have built a model  by following  the instructions in user manual and examples. For post processing, UVARM subroutine was generated by WCM Plug-in. Then we have run our input file with UVARM subroutine; however, we have encountered an error as expressed below;
" Abaqus Error: The executable Standard with system error aborted with system error "illegal memory reference". (signal 11). please check the .dat, .msg, and .sta files for error messages if the files exist." 
Since we have no internet connection at our computer where input files are run, we can not generate system information to report. When we search the error on the internet, common offer for solving problem is avoiding parallelization but we have tried it with one cpu and we have encountered with same problem again. 
By the way, to compile and run our model, we made some changes in UVARM subroutine file. It is that logical definitions were changed into integer definitions (e.g. false->0 and true->1) because we use gfortran as compiler.
Could you please inform us about the possible solutions to our problem? We're looking forward to your reply. Thank you in advance.
Best Regards,
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Hi, does this error persist if you use another compiler?
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Abaqus Error: The executable package.exe
aborted with system error code 1073741819.
Please check the .dat, .msg, and .sta files for error messages if the files
exist. If there are no error messages and you cannot resolve the problem,
please run the command "abaqus job=support information=support" to report and
save your system information. Use the same command to run Abaqus that you
used when the problem occurred. Please contact your local Abaqus support
office and send them the input file, the file support.log which you just
created, the executable name, and the error code.
Abaqus/Analysis exited with errors
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The subroutine did compile successfully on HPC as it was giving problems on local desktop.
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Biolegend Cell Surface Immunofluorescence Staining Protocol uses 15 ml of cell staing buffer to resuspend the cell. Is it necessary? Here is the link: https://www.biolegend.com/protocols/cell-surface-immunofluorescence-staining-protocol/4283/
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Dear Dr. Di Yang ,
Don't bother about 15 ml. Based on the amount of cell suspension you have, add 5 times more PBS + 2% FBS and wash . It works beautifully. I have done >1000 times. Feel free to knock me for further assistance @ mdbabumia777@gmail.com
BR,
Babu M.
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I am doing O2/CO2 research on Combustion and for that I recently need to run some tests on TGA machine model STA 449-F3. My query concerns the selection of Purge gas 1 on the software. This TGA allows me to select different gases only on Purge 1 while Purge 2 is fixed for a very few options. In my case, I have to use a mix of Oxygen and CO2. I have used 2 controller and a flow meters to mix the the gases in the required composition and then attaching to the input of Purge 1.
Now, my question is, does the selection of a Purge gas 1 on the software, has any effect on my results, where if the input mix remains the same?
like if I choose Oxygen as P1 on the software and input mix in actuality is 30% O2/CO2?
Secondly if I choose a previously defined selection option, in the software, of 30% O2/CO2 having density of 1.82 (as shown by the software) and still the input is the same as before, 30% O2/CO2, will the software change the results depending upon which P1 I have selected?
Are the results in this particular TGA, as per received cases?
Does any of the above assumptions and changes have effect on FTIR results?
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According to the manual:
The STA 449 F3 features three different gas inlets which are named purge 1, purge 2 and protective.
The protective gas, as the same implies, is connected to the balance system and protects it against critical sample out-gassing products. The selection for the protective gas is limited to mainly inert gases (N2, Ar, He), Air and dry CO2.
The purge gases can be understood as "sample gases" as they are only in contact with the sample room. Based on your instrument version (corrosive or non-corrosive) the allowed gas selection is different. A detailed list is provided in the manual. However, it includes far more gases than the protective inlet.
Regarding the software it is slightly different. The in-build mass controller is working based on a defined calibration and the physical property of the selected gas mixture. So the system could only provide you the correct flow values as long as you select the correct gas mixture attached to the system. If your mixture is different, the system will control the gas flow but the absolute flow valve isn't correct due to the use of the wrong calibration factor.
This means, you can perform repeatable measurements with the same flow even by selecting the wrong gas mixture within the software. However, you should be aware that in this case the displayed flow values are not the ones which you will have inside of your device.
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Hello all,
I am using FRIC Subroutine and I get the following Error. Anyone has an idea about that?
"The executable standard.exe aborted with system error code 1073741819. Please check the .dat, .msg, and .sta files for error messages if the files exist. If there are no error messages and you cannot resolve the problem, please run the command "abaqus job=support information=support" to report and save your system information. Use the same command to run Abaqus that you used when the problem occurred. Please contact your local Abaqus support office and send them the input file, the file support.log which you just created, the executable name, and the error code."
I changed the file name "mkl_avx2.dll" into " mkl_avx2.dll.11.0.0.1". But it does not still working. Moreover, it is not related to the compiler and so on because it works for the other subroutine codes properly.
Many thanks for your comments.
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Hi
Many thanks to all. I already found the solution. There are two state variable in subroutine so I should have defined in material property " depvar" and given the number as equal as state variable, that is, two.
It was solved.
Regards
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Hello, i'm trying to implement the Paris law in Abaqus. My model consist in a clamped-clamped beam loaded in the middle by a concentrated force. In order to simulate the crack propagation I create an initial crack and i want to propagate the crack trough XFEM and with a low cycle fatigue analysis (direct cyclic). I implement the Paris law in the keyword editor but when i run the model i get these errors at the end of the first cycle:
The system error in std_findcutshape3d8_xfem -- nodal level set values might not be correct for element 509 instance part-2-2
The executable standard.exe aborted with system error code 1073741819. Please check the .dat, .msg, and .sta files for error messages if the files exist. If there are no error messages and you cannot resolve the problem, please run the command "abaqus job=support information=support" to report and save your system information. Use the same command to run Abaqus that you used when the problem occurred. Please contact your local Abaqus support office and send them the input file, the file support.log which you just created, the executable name, and the error code.
Someone know how to fix this error?
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Hi Mohammad, thank you for your suggestions. I try to renamed mkl_avx2.dll to mkl_avx2.dll.11.0.0.1 but the error remain. I realized that when the mesh is less finer the error disappear but the problem is that i need a finer mesh. Did you have any suggestions about mesh size of XFEM? thank you in advance.
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La simulazione nella formazione del personale medico, paramedico ed in genere delle figure che operano a contatto con i pazienti sta acquisendo un ruolo sempre maggiore nella formazione medica, sotto la spinta della maggiore disponibilità di strumenti e ambienti sempre più realistici supportate delle più moderne tecnologie e dell’identificazione di metodi efficaci per il loro uso. Non vi è disciplina o livello della formazione medica universitaria o post-universitaria che non possa trarre giovamento dall’uso delle più moderni strumenti di simulazione, a patto che siano scelte in base a specifici bisogni e non per il gusto della tecnologia. Purtroppo nel nostro paese manca una vera e propria cultura nell’utilizzo di tali strumenti per cui l’introduzione di queste attività dovrebbe essere promossa fino dai primi stadi della formazione medica. La complessità della pratica chirurgica possono ad esempio rappresentare un’ardua sfida per i progetti di formazione e training medico scientifico; le simulazioni interattive ed immersive di fatto accelerano il processo di apprendimento, innovando i modelli di formazione sino ad oggi definiti come tradizionali. Oggi più che mai si sente sempre più parlare di realtà virtuale che applicata alla medicina è in grado di consentire il trasferimento di know-how tra un medico ed il suo allievo, offrendo un elevato grado d’interazione più immediato. La realtà virtuale di fatto rappresenta l’ultima frontiera dei nuovi media, permette di elevare la comunicazione a un livello esperienziale, trasformando l’osservatore nel protagonista della scena. Questa tecnologia si sta facendo varco come applicazione e metodologia pratica in tutti quei contesti in cui l’immedesimazione, il realismo e l’immersione dell’utilizzatore nell’ambiente virtuale rappresenta un valore aggiunto poiché capace di fornire stimoli molto simili a quelli naturali definiti dalla realtà. Stimoli ai quali il medico apprendista tenderà a rispondere parimenti in maniera “naturale”. Tutto ciò rende questa tecnologia lo strumento più potente messo a disposizione per tutto quello che riguarda la simulazione della realtà e la formazione esperienziale specialistica, in cui l’esperienza e pratica devono integrarsi con la parte teorica. I moderni visori indossabili consentono oggi di realizzare prodotti audiovisivi con un livello di realismo senza precedenti, capaci di immergerci al punto da ingannare i nostri stessi sensi. L’integrazione con i sistemi di motion capture, amplificano il senso di presenza durante l’apprendimento, permettendo di interagire con l’ambiente virtuale in modalità naturale e intuitiva. E’ proprio per queste ragioni la realtà virtuale ha come campi applicativi la formazione e la simulazione in campo medico. Inoltre rappresenta uno strumento con grandissime potenzialità per tutto quello che concerne la valutazione di prototipi di protesi e la loro simulazione di ambienti e scenari artificiali. Trasponendo in realtà virtuale modelli e progetti, non solo il medico, quanto il paziente stesso potranno avere una chiara percezione della loro caratteristiche di forma, del loro funzionamento e della loro contestualizzazione in scenari di vita quotidiani come se fossero stati realmente e fisicamente realizzati. A livello mondiale in campo medico la realtà virtuale è ancora in corso di sperimentazione essenzialmente con le applicazioni di training on the job e la cura dei disturbi di ansia e delle fobie. Nel primo caso tipicamente si adopera una telecamera 3D installata in sala operatoria e/o sul medico che sta operando per consentire ad altri di assistere in maniera immersiva all’intervento stesso. Nel secondo, si sfrutta invece il realismo offerto dalla realtà virtuale per ricreare alla presenza di un medico situazioni e scenari capaci d’innescare fobie, attacchi d’ansia, o patologie più gravi quale ad esempio l’autismo, al fine di poter essere meglio interpretate le azioni da intraprendere. La realtà virtuale permetterà ad ogni studente di avventurarsi nell’attività da apprendere, senza una partecipazione distaccata ma pienamente immersiva nel ruolo che dovrà ricoprire. Non si parla solo di visori, infatti, ma di ulteriori dispositivi come guanti speciali che offriranno stimoli tattili permettendo di apprezzare la concretezza dell’intervento, il contatto con un corpo e tutto l’impegno richiesto. Nel prossimo futuro l’economicità del sistema renderà inoltre possibile trasmettere queste esperienze in streaming a migliaia di utenti dovunque nel mondo. Un’opportunità di equità ed omogeneità per la formazione dei professionisti sanitari di tutto il mondo. L’obiettivo del prossimo futuro sarà quello di sviluppare progetti a supporto dell’acquisizione e trasferimento della conoscenza capace di realizzare esperienze fuori dal comune, con un approccio pragmatico ed efficacie. Intellisystem Technologies intende partecipare attivamente alla definizione di nuovi metodi e strumenti per valorizzare queste tecnologie avvalendosi del prezioso supporto e collaborazione delle Università Italiane ed estere.
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Pienamente d'accordo.
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The executable standard.exe aborted with system error code 1073741819. Please check the .dat, .msg, and .sta files for error messages if the files exist. If there are no error messages and you cannot resolve the problem, please run the command "abaqus job=support information=support" to report and save your system information. Use the same command to run Abaqus that you used when the problem occurred. Please contact your local Abaqus support office and send them the input file, the file support.log which you just created, the executable name, and the error code. CAE file is attached below
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Hello
There are some problems which cause this error.
If you have this error in all different models ( you have to check it, first)
The first and common is because of memory. In order to solve it, you can increase the memory in Job modulus ( the default is based on percentage so you can use more than 95% of memory.
Also, in some cases the problem is on the grounds of hardware of system, so you need to run it in another computer ( sometimes MPI and Threads have problem to do analysis based on CPU, memory, etc and cannot parallel )
The last thing may be for the version of ABAQUS you use or its license.
hope it can be useful
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FNAC is an assay which has high sensitivities when optimally selected is efficient and one of the most accurate frontline method for the diagnosis of Tuberculous Lymphadenitis. It is effectively supplemented by Ziehl-Neelsen Staining which is the most simple, quicker, reliable and relatively cheap diagnostic tool .
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@ Dr. Chandan Poddar: I did not want to 'teach' you anyway....
@Prof. Deepthi Nair: thank you for your friendly assessment of my comments /contribution to the matter. I have to admit that I have not studied 'microbiology' as a subject of my university studies long ago... but due to my former profession I found those critters 'next to my eyes' on a TEM (transmission electron microscopy) screen at 3,000 - 20,000 times magnification, when they occured in my human tissue sections to be observed for a diagnostic purposes... at least at that time point I had to learn by myself how to determine and to name those tiny roundish structures... (and therefore I learned a bit about safe and successful collection, as well as about localization techniques (classical, as well as histochemical and Immunohistochemical stainings, PCR, etc...).
Last but not least: I am no Professor and quite informal person (retiree) (just the son of a 98 years old "schoolmistress" and therefore exhibiting 85% of genetic predisposition to indoctrinate others (:-)) .... Best regards, W.M.
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After SNL i harvested the spinal cord segments, Using Trizol reagent i isolated RNA then Protein extraction, measured protein concentration by BCA and had a relatively good concentration. Used the protein for WB. Primary antibody was Iba1, i couldnt get any band. but i had very clear bands when i used GFAP=astrocytes.
On ther hand, after perfusion fixation with 4% PFA, harvested the spinal segment, overnight in PFA, followed by 10,15,20% sucrose. cryopreserved the sections. cut 30um sections and tried to staing Microglia, using the same Iba1, but failed.
Has anyone encountered a similer difficulty in both WB and IHC-F?
What did you do to solve the problem?
Will changing the company help?
Thank you.
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I have had similar problems with imunos using Iba1. I ended up opting to study different microglia subpopulations using specific markers.
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I am starting to write and test VUMAT for my Abaqus simulation. Since mine is a linux system and I can't buy a licensed Intel Visual Fortran Compiler, I am using the default gfortran compiler available in linux. I tested a single material VUMAT and it got compiled. Abaqus was able to read it and use it for analysis.
The problem arises for a multi-material VUMAT. It shows no compilation errors. But when I submit it along with my Abaqus job, Abaqus gives the following error. The .msg file is not formed and the .sta file is empty. 
'Illegal memory reference' (signal 11).
The same VUMAT gets compiled and the input file gets executed when compiled using Intel Visual Fortran compiler on another system.
Please let me know any changes to be made in the gfortran compiler or if additional information is needed.
Thank you.
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Hi Sri Krishna,
I am trying to run a VUMAT subroutine with abaqus 6.14-5 and gfortran as compiler, but I am not succeeding. I have plenty of error messages like:
Error: Non-numeric character in statement label at (1)
...
I just read that you have gfortran working at least when the VUMAT subroutine handles only one material. Do you have any suggestions to solve my problem?
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In our lab, we have a PerkinElmer STA 6000 Thermal analyser. I would like to know, how to analyse our sample using weight percentage measurement of the 'vapourising species'. Kindly give me some examples.
Thank you
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For TGA, it is always the weight loss % vs temperature. So, to know for what the % weight loss corresponds to you need to know your material very well. for eg M(CxHyOz). 2H2O.2 EtOH this is some complex so when you will get first weight loss it is due to 2 ethanol molecules then 2 water molecules and then due to some organic moiety loss. This I can conclude because I already know the temperature where water will go and where ethanol molecules should go. So, for this you need to know the range of temperature where you expect the decomposition of the reactants/ solvents you used. Then only you will be able to analyze your TGA data.
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I am basically doing some experimentation (adsorption in a resin and effect of temperature) using Simultaneous thermal analyser. It can be used for thermogravimetry analysis as well as differential scanning calorimetry. STA gives by default DSC results and i dont know how to evaluate heat of adsorption.
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using DSC, the specific reaction heat (whatever exo- or endothermic reaction this might be) can typically be obtained by subtracting a porpper measured baseline and normalizing of the date by dividing the heat flow signal (in mW) with sample mass (in mg) and scanning rate in K/s - the resulting unit is J/(g*K). Integration as function of temperature of the peak area then results in the specific reaction heat in J/g. The tricky thing might be to find the propper zero level for integration. Good luck!
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I am modeling PZT patch in ABAQUS 6.14 involving explicit-implicit co-simulation? The error shown is:
Error in job Co-execution-1-Explicit: The executable explicit_dp.exe aborted with system error code 3. Please check the .dat, .msg, and .sta files for error messages if the files exist. If there are no error messages and you cannot resolve the problem, please run the command "abaqus job=support information=support" to report and save your system information. Use the same command to run Abaqus that you used when the problem occurred. Please contact your local Abaqus support office and send them the input file, the file support.log which you just created, the executable name, and the error code.
Job Co-execution-1-Explicit aborted due to errors.
ERROR in job messaging system: Error in connection to analysis
Error in job Co-execution-1-Implicit: Abaqus/Standard Analysis exited with an error - Please see the message file for possible error messages if the file exists.
Job Co-execution-1-Implicit aborted due to errors.
Can anyone help me with it?
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The system error codes are error codes from windows.
error code 3 refer to PATH_NOT_FOUND
I think some of your paths in your co simulation are not correct. Sometimes Abaqus has problems if two processes try to get or write data in the same folder. Especially if you run your simulation with multiple cores.
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for example HCl (g) + Al (S,powder). STA or Online GC method?
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Thank you very much for answers but how used of Calorimetry for this system?
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I am doing research on TGA and DSC but i cannot seem to find the advantages and disadvantages of Simultaneous Thermal Analysis.
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Advantages of TGA-DSC/DTA(STA): 
  • Your temperature profile, atmosphere, sample, etc are identical for both data sets - it eliminates uncertainty associated with differences in sample preparation, sampling, differing instruments and furnace materials, etc. 
  • By employing STA, you get two experiments with the time and sample of one - this is very attractive for researchers into carbon allotropes and noble metals, where 1g of sample can be very expensive, and for those researchers who have difficult-to-prepare samples. 
  • Because both data sets are collected on the same sample with the same ramp in the same furnace with the same crucibles under the same gas flow -i.e., because all possible confounders are controlled for - STA allows better correlation of time/heat flow and time/mass change phenomena in time-sensitive operations. It therefore would be my technique of choice for studying kinetic phenomena, like protein folding, among others. 
Disadvantages of TGA-DSC/DTA
  • Very often you sacrifice sample size (and consequently, sensitivity to small mass loss effects). Exception is in the case of systems which employ 3D (Calvet) DSC sensors which may accommodate relatively large DSC samples, like the SETARAM Sensys Evolution. But in the vast majority of cases, a TG-DSC system cannot accommodate as large a sample as a standalone TGA. 
  • Likewise (especially in TG-DTA systems) you often sacrifice heat flow sensitivity (and thus the ability to observe small heat effects). Some systems do not have to sacrifice this, but you pay for the added sensitivity in higher capital cost - but for those who need it, the investment is worth it, and one high-end STA system typically costs less than a separate TGA and DSC of similar sensitivity. 
  • Finally, there is the issue of putting all of one's thermochemical eggs in a single instrument basket (please forgive me if you're not familiar with that English idiom) - compared with having a separate TGA and DSC, if your STA instrument breaks down, you're in big trouble because you can't do either TGA or DSC. For most people, however, the versatility of doing both more than makes up for this risk. 
To use a simile: The difference between STA and standalone TGA and DSC is rather like the difference between having a multitool and a standalone pair of pliers and screwdriver. If you want a multitool that will be pliers and screwdriver as well as a standalone pair of pliers and screwdriver, it's going to cost a lot of money. A low end one will do both, but it will do neither as well as a separate pair of pliers and screwdriver would. That said, for some, the versatility of doing both makes up for the poorer performance. And, of course, you can always pay a lot of money if you want one that will do both very well. And you're always running the risk of the fact that if you depend only on your multitool, you might be in trouble if it breaks. 
Does that help?
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I have a question on how to calculate IC50 of drug treatment on cell survival.
I did three independent experiments in 96--well plates using the MTT method. After putting the OD values into Prisms one experiment at a time, I got three different IC50s. From these I got an averaged IC50. However, when you want to present the fitted survival curve, you can only choose one representative curve from the three, right?
In order to show a curve which will incorporate all data from the three independent experiments, I did the following and I want to know if this is acceptable. For experiment 1, drug concentration 1, I averaged the OD values from triplicate wells and normalized it to control (100% survival), and got about 10% survival. I did the same thing for exp. 2 & 3, calculated their survivals at this drug concentration, say 11%, 12% and similarly calculated survival for all the other drug concentrations. This way, the data input for Prism would be: Drug Conc. 1, 10%, 11%, 12%; (each is the average from one independent exp.) Drug Conc. 2: 20%, 21%, 22%, etc. Now, Prism will only return one fitted curve and one IC50 value. Is this data manipulation acceptable? Thanks!!
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The notion that you cherry-pick the best data is not a robust scientifc method! Unless you have good reason to discard an experimental data set as flawed, the data should be included in your final analysis. The weighted mean approach is mathematically weighted towards the means with the lowest standard error to account for biological variation.