Questions related to Squamous Cell Carcinoma
Does anyone here have a protocol for the isolation and culture of primary head and neck squamous cell carcinoma cells?
I am working with the SCC-15 (CRL-1623) cell line that I purchased from ATCC. I carefully follow the protocol recommended at ATCC . However, the cells are growing very slowly. Is growth always slow? How can I accelerate growth?
I have identified some over-expressed genes associated with head and neck squamous cell carcinoma by using bioinformatics analysis. Now, I want to validate those genes at protein levels by showing IHC results available on the Human Protein Atlas database. The problem is that I can find cancerous tissues, but not normal tissues to compare with. For example, the IHC results for the GBP1 gene shows just for tumor tissues. If anyone has experience with that, please reply. Thank you.
My present project is related to oral carcinoma cell line, for which I wanted to inquire about any lab or institute that might be working on this cell line presently and if somehow I can get the working flask or cryovial for the same.
I have to start a study about a comparison between the metabolism of Human TONGUE Epithelial Cells (healthy cells) and human tongue squamous carcinoma cell line, after treatments.
I have the human tongue squamous carcinoma cell line but I need to buy Human TONGUE Epithelial Cells (healthy cells). Can you suggest a company where it is possible to find them? I am in Italy.
Thank you so much for your support
Basal cell carcinoma (BCC) is the most common form of skin cancer. An estimated 4.3 million cases of BCC are diagnosed in the U.S. each year. Squamous cell carcinoma (SCC) is the second most common form of skin cancer.
I am planning to do an MTT assay for the cytotoxicity evaluation of several plant extracts on A431 cell line (human epidermoid carcinoma). For this I need to use a positive standard. In the past I have used staurosporine, but it is very expensive (I am PhD Student :) ). Are there other substances that I can use that have proven to have a cytotoxic effect on this cell line? Thanks and best regards! Iolanda
i want to buy Human Squamous Cell Carcinoma Cell Lines for my PhD research .?
One place is NCCS Pune...Can some give more suggestions..? Humble Regards
A CASE OF recurrent sqamous cell carcinoma lower third esophagus. She received radiochemo. in the 1st presentation 3 years ago. Does she need a 2nd neoadjuvant therapy prior to surgery?
What is meant by complete response to radiochemotherapy and how many biopsies are needed to prove this? and is there any role for surgery in non complicted complete responders to radio or chemo?
I am in a situation where I need to freeze down freshly dissociated primary human squamous cell carcinoma cells for later use. Does anyone have any reccomendations on what media/solutions I can use for freezing to maximise cell viability and minimise cell loss when I thaw them out in the future?
Assume there is a patient with oral tongue SCC (pT3N1) underwent surgery (R0) and postop con. ChemoRT with weekly cisplatin. One month after completion a reccurence emerges in the radiated field. You want to start palliative chemo. Do you consider this patient as resistant to Cisplatin and choose other non-cis drugs? or Add something to Cis?
Has anyone transfected anf of these cell lines and can suggest a protocol? We need a cell line that expresses a certain gene to try our fluorescent construct on, so we don´t mind which type of cell line it is as long as it expresses the gene we need. We already checked that, they all express the gene, but now we would like to know which one should we try first, which one is easier to transfect and more efficient. If you could suggest protocols or good cell lines in this list that would be awesome.
- IMR-32 Human neuroblastoma cell line Neuroblast Brain.
- HEK 293 Human embrionic kidney cell line Epithelial Kidney.
- HT29 Human colon adenocarcinoma cell line Epithelial Colon
- A-549 Human adenocarcinomic alveolar basal epithelial cell line Epithelial Lung
- Hep G2 Human hepatocellular carcinoma cell line Epithelial Liver
- Hepa RG
- JURKAT Human T cell lymphoblast-like cell line Lymphoblast T lymphocyt
- A-431 Human epidermoid carcinoma cell line Epithelial Skin
- HCT116 Human colorectal carcinoma Epithelial Colon
Cancer of the esophagus is a fatal cancer. There are two histological types of esophageal cancer, namely; adeno carcinoma and squamous cell carcinoma (SCC). The incidence of SCC esophagus is common in the Asian continent and in the North Eastern part of India. Radiotherapy is the standard modality of treatment in unresectable SCC of the esophagus and chemotherapy is administered as an adjuvant or concurrent to radiotherapy. Concurrent chemo-radiotherapy (CRT) is ideal for patients with resectable SCC of the esophagus who refuse surgical resection, and also in locally advanced unresectable cases . The treatment outcomes of SCC esophagus show a high degree of residual diseases after radiotherapy alone or after CRT. However, a sub-group of esophageal SCC responds very well to a standard 50-60Gray of external beam radiotherapy with chemotherapy, and many a times without chemotherapy as well. It is seen that those malignancies that responded well to the radiotherapy initially, when they recurred after a period of two to three years, it was radio-resistant later !! Why does this happen ??
In a patient, squamous cell carcinoma with metastasis in the region of S1 S3. What can be the solution for the treatment of pain in the leg, the sacrum in the back.
Thanks for your time for me. My query is on the radiation therapy process for a cancer patient.
How radiation simulation and marking process is done prior to radiation therapy for a cancer patient?
Any kind of explanatory video /animation/ documents from the basic level is best for me to understand it as I have absolute zero knowledge about the whole process.
In treatment of non-small cell carcinoma differentiating adeno from squamous cell carcinoma is essential.When morphological diagnosis is difficult IHC is done and sometimes we get adeno and squamous cell marker co-expression by same group of cells.
Preferably using Matrigel as the matrix to be invaded. Time of incubation in serum starved condition, time of culture and concentration of Matrigel bed is what I am looking for.
The paper from Boonstra et al. 2007 (DOI: 10.1158/0008-5472.CAN-07-2064) showed that several human esophageal carcinoma cell lines were misidentifyed.
His conclusion was that TE-2, TE-3, TE-7, TE-12, and TE-13 should be regarded as one single squamous cell carcinoma cell line, however, he did not rename them.
We have been working with TE-7 and TE-13, and confirmed his results that they are genetically identical.
We have been looking for information on these cells in several Cell Banks, but they were either absent or still named as separate cell lines. Anyone knows how these cell lines have been named?
I am searching an appropriate primer for diagnosis of vaginal cancers in female dromedary camels. I encountered some cases with adenocarcinoma, squamous cell carcinoma and some other types of vaginal and cervical cancers. From literature (About half of vaginal cancers in humans is associated with human papillomavirus (HPV) detection, in contrast to cervix cancer which is essentially all associated with HPV). Papillomaviruses have been detected in camels. I tried to test two samples by Prof Robert D Burk (Albert Einstein College of Medicine - NY-USA), but I had a problem in sample transportation. Now, I would like to test the other cases in home, but I do not know what is the appropriate primer that should be used.
In my institution, no routinely assessment of Human Papillomavirus (HPV) is performed in the histological examination. In relation to recent knowledge within the pathogenic role of HPV in the developing of oral squamous cell carcinoma (OSCC) and even in its prognosis, one should ask if it should be mandatory in all post-op histollogical reports, in order to administer or not complimentary treatment. What is your experience and/or thinking on it?
I received reasonable number of photos on breast from our colleagues in Research Gate.
I am looking for following as well, please see whether you can contribute. You will be acknowledged.
1. Nipple or breast piercing/ and their complications
2. tattooing in breast skin
3. breast implants and complications
4. developmental defects in breast
5. psoriasis, hydradenitis in submammary area/ breast skin
This baby was born by elective LSCS and detected to have mild tachypnoea, which improved within 48hrs. X ray was showing a mediastinal mass and was referred to us. He was diagnosed to have mediastinal neuroblastoma (Biopsy proven), N myc negative, Stage III.
I have been generating spheroids from HNSCC cell lines. During spheroid formation, some of the cells may not form spheroids. So how can we seperate these single cells and collect only spheroids for further experiments? Can we do differential centrifugation? If yes, what is the speed required?
I would like to analyse proapoptotic activity of a compound to be used on Actinic keratosis. Is there any cell line that might represent early phases of photocarcinogenesis?
what is the media too, because i found two different medias one with growth factors and the other with FBS?
working on SCC12 cell line
where i can get the Matrigel?
In my present study, I am studying the expression of three genes (a,b,c) in NSCLC cases. My goal is to find out if I can classify NSCLC into adenocarcinoma and squamous cell carcinoma based on the Ct value.
1. First I have normalized the Ct value by subtracting the Ct value of endogenous control.
2. I have used a formula to get a score (s) for each case for each gene.
1. Should I compare original Ct value or normalized Ct value to understand if there were any significant difference between adenocarcinoma or sqouamous carcinoma for each gene? Which test should I perform (t test or mann-whitney test)?
2. Also, I want to investigate that, if there were any significant difference in Score (S) between adeno and squamous carcinoma for each gene. (Score is derieved from normalised Ct). (Again, should I use t test or mann-whitney test)?
I am working on chemoresistance of human squamous cell carcinoma (SCC12) in the context of tumor microenvrionment, CAFs specifically.
I have already done experiments with different concentrations of each drug (cisplatin, docetaxel and 5-FU) ranging from 0-120uM for cisplatin, 0-100uM for docetaxel and 0-500uM for 5-FU.
I am wondering does anybody have experience with these drugs to tell me what are the physiological concentrations in patients? I have checked for concentration in plasma but I could not find a consistent answer.
I would like my results to reflect the conditions in patients.
Does anyone have knowledge or experience dealing with the effects of testosterone, estrogen and/or aromatase inhibitors on squamous cell cancer of the thymus. We are involved in a case where the liquid biopsy tested positive for both estrogen and androgen receptors.and are trying to understand the possible connection and therapeutic implication
i read about tumor marker and enjoy about its very important role in early diagnosis and help in assessment of therapy and recurrence probability,so i need to know if there is specific tumor marker for SCC effecting oral mucosa?
I try to find CAFs of squamous cell carcinoma of the head and neck or lung. I found one company in the US selling CAFs of lunch squamous cell carcinoma but they don't deliver them in Europe. Do you know if it is possible to obtain such CAFs from other research institutes or commercially?
Many thanks! Have a nice WE,
We have a quick (in vivo) biopsy to lab transfer time, we wash three times, use multiple types of medium with different concentrations of antibiotic, EGF, cholera toxin etc. Others in the lab have also used irradiated 3T3 feeder layers.
Any tips or is it just a matter of needing large numbers? We must have explanted 50 different patient samples by now.
How do you handle growth delay data from mice that develop ulceration over their SC tumors following rapid growth rate; include data until exclusion or totally exclude data?
I'm looking for a rat oral cancer cell line to produce tumor in the oral cavity (preferably in the tongue) of healthy (immunocompetent) SD or Wistar rats. For Eg: oral squamous cell carcinoma cells.
It would be helpful if you can answer any or all of the following:
1. Do you have/know a cell line which I can use.
2. Can a cell line derived from a different strain used? For Eg: A cell line derived from Wistar rat can be used to produce tumor in SD rats?
3. Any method to transform healthy cells to tumor cells and use them for syngeneic tumor development?
4. Mention any special points / precautions to be considered to develop this type of syngeneic tumor model.
30 year old gentleman, squamous cell carcinoma tongue,
excised at a peripheral hospital 3 month back; no neck dissection done.
HER - pT1, RIGHT lateral border, tumor thickness 0.2 cm, all margins close.
No adjuvant therapy received.
Now patient has LEFT level 1, hard, fixed node; RIGHT neck - no nodes; Tongue - NO LESION FELT.
FNAC of RIGHT neck node -SCC
MRI tongue - ill defined lesion 0.5 cm along LEFT lateral border; to co relate clinically.
How will you proceed?
IN recent times.i.e. in the last ten years, there have been numerous case reports on Head and Neck Squamous Cell Carcinoma which may have HPV as one of the contributing/causative agents.
I have also seen one patient with OSCC wherein there was no history of tobacco usage., and/or trauma/ sharp teeth etc.
I want inputs from all those doctors/researchers/health care workers/caregivers who have seen similar cases.
I am attempting to me a RDEB patient SCC cell line from a frozen tissue sample. I have a couple of protocols saved but none of them are specific to SCC or RDEB.
Thank you for any help you can provide!
In many Arab countries, obstructive uropathy constitutes a major cause (40%) of end stage renal disease mostly due to renal calculi and schistosomiasis. Schistosomiasis, due to S. mansoni and S. haematobium, is an endemic common disease in a number of Arab countries. Both types have their negative impact on the kidneys. While obstructive uropathy is a common complication of S. haematobium, distinct patterns of glomerular lesions, including membranoproliferative glomerulonephritis and focal segmental glomerulosclerosis, are associated with infection by S. mansoni or S. japonicum. However, with the widespread use of praziquantel, the prevalence of Schistosomiasis decreased over time. In Egypt, the incidence of squamous cell carcinoma of the bladder decreased while that of transitional cell carcinoma increased after mass treatment of Schistosomiasis. In South America, a change in the distribution of glomerulopathies associated with nephrotic syndrome was observed along with a decline in the occurrence of severe forms of schistosomiasis.
Can anyone tell me about positive control tissue of c-fos in Immunohistochemistry for human oral squamous cell carcinoma. Please help me.
I am looking for squamous metaplasia in human tissue. I wonder if anybody used a specific marker (antibody) to identify metaplastic squamous cells by immunohistochemistry.
Is it possible to define any relationship between cutaneous squamous cell ca and atrial myxoma? We have seen both tumors in the same patient. We haven' t seen any report about this simultaneous occurance. However there are some reports about the simultaneous occurance of the squamous cell ca of the lung and myxoma.
In very severe psoriasis patient with currently diagnosed squamous cell carcinoma,
should ignore the dermatological problem? because PUVA side effect is SCC and that patient is already resistant to other topical treatment.
I am doing research for my EBP question . Any suggestion please.
Three patients with pulmonary squamous cells carcinomas submitted to pneumonectomy had relapses in single cervical lymphnodes respectively after three, nine and twelve months after the operation. We undertook radical monolateral cervical lymphadenectomies and the three years follow up didn't detect any relapse in all the cases.
Five-year survival of oral cancer varies from 81% for patients with localized disease to 42% for those with regional disease and to 17% if distant metastases are present. Generally, due to late-stage diagnosis, fewer than 50% of patients with oral and pharyngeal cancers survive more than 5 years. This rate has remained disappointingly low and relatively constant during the last few decades. Therefore there is need to create awareness for oral cancer screening so that the deadly disease can be diagnosed at an early stage.
I am doing research in oral cancer. I need any specific marker present for Oral squamous cell carcinoma. Please kindly send me these details.
Based on the surface area of about 1.9 m2 and 3.5 million new cancers per year (Squamous cell carcinoma and basal cell carcinoma) the ectocervix has a very high cancer rate per m2 surface area: about 0.00075 m2 and about 12000 new cases per year. If you oversimplify and calculate the ratio of tumors/m2 in the USA you get enormous differences. Do these differences represent a higher susceptibility of the ectocervix or cervix compared to epidermis; or a different exposure to carcinogens (in case of the ectocervix HPV)?
Within the skin, are melanoma rare or more common than epidermal tumors taken into account the low number of melanocytes compared to keratinocytes (they at least are exposed to the same carcinogen)?
I want to down-regulate gene of interest in SCC cells but this cells are hard to transfect. I have tried using Xtreme gene as well but got 10-15% transfection.
Seeing only some early esophageal Ca cryotherapy publications. Also, who is the best current manufacturer of the device? Any recommendations?
After primary culture from one tumor sample (of squamous cell carcinoma of cattle ) I found a lot of small round shining apparently nucleated (fine adjusted inverted microscope) cells partly in suspension & partly attached to collagen coated t-25 flasks on the consecutive 3rd & 4th day. Before processing the primary culture the tumor tissue was well detached from vascular tissues. These cells are also increasing in number. When I performed percoll gradient differentiation of cells collected from the flask at different gradient (30%,40,60,80,90%) the cells took the gradient in between 30-40%. Can these be stem cells? Or rather should I ask how to get stem cells from this type of sample? Seeded them in gelatin coated flasks.
Most of these are squamous cell carcinomas, and very well-differentiated, with lymph nodes of neck also involved.
There is always a boy-friend or girl-friend in the picture.