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Liquid storage is the primary preservation method in the swine breeding industry because of its advantages over cryopreservation. Calcium (Ca²⁺), a key regulator of cell physiology, plays a crucial role during liquid preservation. Sarcoplasmic/Endoplasmic Reticulum Ca²⁺ ATPases (SERCA) belong to a family of P-type ATPases that regulate Ca²⁺ homeost...
Article
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Background To the best of our knowledge, there is a gap in the review of literature about the most suitable varicocelectomy technique in isolating and ligating adherent periarterial vein(s). Consequently, leaving the artery intact or ligating it together with the adherent vein may pose a challenge. We conducted a comparative prospective study to as...
Article
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Species coexistence is shaped by a range of biotic and abiotic factors. Beyond predation, parasitism and competition, one species may interfere with another's reproduction to induce sexual exclusion from a habitat. Here, we test for reproductive interference from inter‐species mating between sympatric nematodes Caenorhabditis macrosperma and C. nou...
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The main objective of this study was to investigate boar-to-boar variations in the quality characteristics of sperm from the sperm-rich fractions (SRFs) and whole ejaculates (WEs) following freezing–thawing. Several sperm attributes, such as motility patterns analyzed by the computer-assisted sperm analysis (CASA) system, mitochondrial function, me...
Article
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Objective To evaluate the impact of vitamin E supplementation on sperm analysis results in patients post-varicocelectomy. Martials and method This single-center, triple-blind, randomized controlled trial was conducted at Imam Reza Hospital, Mashhad, Iran. Ninety male patients, aged 15–25 years, with infertility and varicocele grade 2 or 3, were ra...
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This article delves into the legal developments, bioethical nuances, and societal perspectives surrounding posthumous sperm use, particularly in the context of soldiers falling during their service. It analyzes the Israeli example during The Swords of Iron War, where the bioethical dilemma is decided based on national solidarity, beyond the “clean”...
Article
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Nonhuman primates (NHPs) are in increasing demand as models for preclinical research. The availability of a highly efficient cryopreservation protocol for use with NHP sperm has potential to significantly alleviate this demand and contribute to rapid increases in overall number of NHPs available for biomedical research. Thus, this systematic review...
Article
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Objective To explore the correlation between antinuclear antibodies (ANAs) and pregnancy loss (PL), and to observe its impact on the pregnancy outcomes of in vitro fertilization/intracytoplasmic sperm injection‐embryo transfer (IVF/ICSI‐ET) in recurrent PL (RPL) patients. Methods In this retrospective study, patients first seen at the hospital bet...
Article
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Infertility is one of the most common diseases in the world in last period of all types, whether it is due to (asthenospermia,oligoasthenospermia,teratospermia,hormonal imbalance). Study aims to reveal negative effect of sperm DNA fragmentation on fertilization, this study was conducted in the Al-Jazeera Specialized Laboratory in the city of Kut fo...
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Sperm navigation through the complex microarchitecture of the fallopian tube is essential for the successful fertilization. Spatiotemporal structural alteration due to folded epithelium or the muscle contractions in the fallopian...
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External female genital mutilation (EFGM) is a type of traumatic mating in which males damage female genitalia, resulting in the loss of female re-mating ability. This study examined whether sexual conflict underlies EFGM by examining the possible female reproductive costs from the decreased number of matings in spider, Cyclosa argenteoalba. The fe...
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Background: Zinc (Zn) is an essential metal that plays a critical role in normal testicular development, spermatogenesis, prevention of sperm degradation, and overall male fertility. This review aims to offer a comprehensive and current overview of seminal plasma Zn levels in fertile men worldwide. It also aims to compare Zn levels in seminal plasm...
Article
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Poor male fertility significantly affects dairy production, primarily due to low conception rates (CR) in bulls, even when cows are inseminated with morphologically normal sperm. Seminal plasma is a key factor in evaluating the fertilizing ability of bull semen. The extracellular vesicles (EVs) in seminal plasma contain fertility-associated protein...
Article
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Human papillomavirus (HPV) infection is one of the most common sexually transmitted infections in all genders worldwide. Its association with male infertility is deeply investigated, although there are conflicting data on the role of the virus in the impairment of semen quality and reduced reproductive outcomes. In this study, we considered 335 sem...
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To assess whether infertile women’s psychological conditions, such as depression, anxiety, and stress, are associated with in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) outcomes, we systematically searched for relevant articles from January 1, 2004, to March 29, 2024, in five databases, including PubMed, Web of Science, E...
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The effects of chronically stressing male mice can be transmitted across generations by stress-specific changes in their sperm miRNA content, which induce stress-specific phenotypes in their offspring. However, how each stress paradigm alters the levels of distinct sets of sperm miRNAs is not known. We showed previously that exposure of male mice t...
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Islam is a diverse religion that fosters and accommodates technological advances including in the field of medicine, but with certain limitations and constraints. This article examines the intersection of motherhood, Islamic law (Shari’ah), and Assisted Reproductive Technologies (ART) through a comprehensive jurisprudential analysis. With the advan...
Preprint
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Genetic engineering, robotics engineering, cryopreservation and spacecraft with nuclear fuel can help us to send human civilization from the earth to exoplanets. Af first, we have select of habitable exoplanets. Then we have to preserve sperm and eggs (or preserved tissue of human body) of human by cryopreservation in a spacecraft (used nuclear fue...
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The neurotransmitter, 5-hydroxytriptamine (5-HT), is well known. Furthermore, it enhances the acrosome reaction, hyperactivation, and in vitro fertilization (IVF) success in hamsters and mice. In the present study, we examined whether 5-HT enhances hyperactivation and increases IVF success in rats. When rat sperm was exposed to 5-HT, hyperactivatio...
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Limited trace mineral research has focused on the impacts of maternal trace mineral supplementation on offspring fertility. We hypothesized that maternal injectable mineral (INJ) administration during mid/late gestation would improve overall growth and reproductive parameters in offspring. Two-hundred and seventy-eight cows were enrolled in the stu...
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Objective Abstinence time has been associated with semen quality, but the results remain controversial. Methods This study recruited 3052 men undergoing fertility evaluation. Abstinence time (AT) was categorized as short (0-1 day), WHO-recommended (2-7 days) and long (>7 days). Semen parameters including volume, sperm concentration, progressive mo...
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Introduction Vasectomy is a widely used, safe, effective method of permanent contraception and contributes to healthy sexuality. Aims We have conducted a 3-step observational clinical study to develop a vasectomy regret risk score and guide patients and clinicians when discussing a vasectomy. Methods A 3-step approach has been followed. First, ex...
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Background: Varicocele (Vx) which is the most treatable cause of male infertility, is also associated with low sperm count, decreased sperm motility and increased sperm abnormal morphology. We aimed in the current study to evaluate the correlation between seminal α-Glycerylphosphorylcholine (αGPC) and semen parameters in infertile patients pre- and...
Preprint
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A protein complex formed by a sperm protein (SPE-11) and and oocyte protein (OOPS-1) functions as an actin regulator to control multiple post-fertilization developmental events, including the completion of meiosis, the block to polyspermy, and egg activation.
Conference Paper
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The hypo-osmotic swelling test (HOST) is used to evaluate the integrity of the plasma membrane for human and other domestic animal spermatozoa. This study was conducted on spermatozoa of three species, including pigs, rabbits, and goats. Three experiments were conducted on three animal species (pigs, rabbits, and goats) to investigate the factors a...
Article
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Introduction Cryopreservation of poultry sperm is crucial for preserving genetic diversity and protecting endangered breeds. Rooster sperm is highly sensitive to cryopreservation due to its high polyunsaturated fatty acid content, making it prone to damage during freezing and thawing. This study evaluated the effects of thawing temperatures and sto...
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In plants, sperm cell formation involves two rounds of pollen mitoses, in which the microspore initiates the first pollen mitosis (PMI) to produce a vegetative cell and a generative cell, then the generative cell continues the second mitosis (PMII) to produce two sperm cells. DUO1, a R2R3 Myb transcription factor, is activated in the generative cel...
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The research and development of a matrix of Addressable Nanoliter Containers (ANLCs) is the focus of this work. ANLCs introduce a novel approach for cryopreserving single sperm cells. A significant increase in sperm cell mortality was observed after cryopreserving nanoliter-scale cell suspensions, attributed to the diffusion of water from the aqueo...
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Introduction Recurrent pregnancy loss (RPL), defined as two or more consecutive pregnancy losses before 24 weeks of gestation, affects up to 1%–2% of couples. Aim of this retrospective cohort study was to report the main causes and pregnancy outcomes of a cohort of women with RPL and the efficacy of a personalized work‐up and treatment in terms of...
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Ensuring boar sperm quality before insemination is crucial for maximizing field fertility and efficient pig production. The computer-assisted sperm analysis (CASA) and fluorescence probes combined with flow cytometry (FC) are commonly used techniques for evaluating sperm kinematics and functions, closely related to animal fertility. However, their...
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Purpose To investigate the therapeutic potential of Acrorus tatarinowii in oligoasthenozoospermia and its related mechanism through modulation of the BCL2/Bax/Caspase3 signaling pathway. Methods Initially, using the TCMSP and Disgenet databases, active ingredients of Acrorus tatarinowii were identified and their target genes associated with sperm-...
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The aim of this work is to improve the reproductive performance of grasscutters in captivity. Thirty (30) grasscutters (Thryonomys swinderianus) aged seven (7) months were selected. They were divided into (two) 2 batches (batch 1 and batch 2) of fifteen individuals in order to receive distinctly (two) 2 types of food. Batch 1 and batch 2 received f...
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Conventional microscopy‐driven sperm health monitoring systems suffer from high infrastructural costs and complex protocols. Here, a simple, economical sperm motility assessment system is proposed. Based on a cantilever‐deflection‐based direct force measurement system capable of detecting wetting forces in the range of ≈µN, it is found that the adh...
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Reproduction in males is one of the complicated processes that is mediated by many environmental factors, as well as by diet (e.g. supplements, nutritional value). The parameters of reproduction like sperm cells, hormones and testicular histology are highly prone to oxidative stress due to influence of factors on reproductive processes. Polyphenols...
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Over the past two decades, the study of sperm-borne small non-coding RNAs (sncRNAs) has garnered substantial growth. Once considered mere byproducts during germ cell maturation, these sncRNAs have now been recognized as crucial carriers of epigenetic information, playing a significant role in transmitting acquired traits from paternal to offspring,...
Article
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Residents of the oil rich city of Port Harcourt and its environs have been inundated since November 2016 by a fresh wave of pollution plumes of thick black soot in the air capable of impacting health and wellbeing of the people. The current study investigated the relationship between exposure to environmental black soot and some reproductive and te...
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Background In the cryopreservation process, rooster spermatozoa are vastly sensitive to reactive oxygen species (ROS). This study aimed to investigate the effects of Lake extender supplemented via Cysteamine (CYS) on the quality and fertility characteristics of rooster semen during the cryopreservation process. Methods Semen samples were collected...
Preprint
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The combination of different imaging modalities into single imaging platforms has a strong potential in biomedical sciences since it permits the analysis of complementary properties of the target sample. Here, we report on an extremely simple, cost-effective, and compact microscope platform for achieving simultaneous fluorescence and quantitative p...
Article
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Maintenance and breeding of experimental organisms are fundamental to life sciences, but both initial and running costs, and hands-on zootechnical demands can be challenging for many laboratories. Here, we first aimed to further develop a simple protocol for reliable inland culture of tunicate model species of the Ciona genus. We cultured both Cion...
Conference Paper
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Buffalo sperm are sensitive to peroxidation of lipids because buffalo spermatozoa contain highly polyunsaturated free fatty acid (PUFA) which reduces the bull fertility following cryopreservation compared to other species. The current study was carried out to determine the effect of vitamin K supplementation in tris extender on Iraqi buffalo semen...
Article
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Sertoli cells (SCs), as the somatic cells in the testis of male mammals, play a crucial role in the close association with germ cells. The blood-testicular barrier (BTB), established by their tight junctions, provides immune protection to germ cells, leading to their characterization as "sentinel" cells. Moreover, the physiological process of testi...
Article
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In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are essential techniques in assisted reproductive technology (ART), yet poor ovarian response remains a significant challenge. For patients with poor ovarian reserve, traditional stimulation protocols often lead to suboptimal outcomes. The Shanghai Protocol, which involves dou...
Article
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Objective To explore the effects of donor sperm on reproductive quality of life, sleep quality and erectile function in patients with severe oligoasthenospermia after the failure of intracytoplasmic sperm injection (ICSI), and to provide targeted reference for intervention. Methods From January 2021 to December 2023, patients with severe oligoasth...
Article
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Preserving fertility is important in men under radiation therapy because healthy cells are also affected by radiation. Supplementation with antioxidants is a controversial issue in this process. Designing a biocompatible delivery system containing hydrophobic antioxidants to release control may solve these disagreements. Herein, a bifunctional peri...
Article
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Acute lymphoblastic leukemia (ALL) is a malignant condition of lymphoid progenitor cells that primarily affects the pediatric population, but also adults. The 5-year survival rate is 90% in children and approximately 40% in adults, with survival increasing through the use of peripheral stem cell allotransplantation (SCT). The relapse rate after ste...
Article
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Semen quality determines the fertilizing ability of cocks and other male animal's semen. The goal of this study was to ascertain how ecotype, collection week, cock, and their interactions affected semen quality. The study also estimated the repeatability of some semen quality traits. A total of forty (40) cocks made up of 20 heavy ecotypes and 20 l...
Article
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Copulation is essential for transferring sperm from males to females in most animals. During copulation, males and females are often static and thus prone to predation or other threats. Its duration should therefore be reduced to minimize costs but sufficient to ensure the fertilization of eggs. Here, we investigated reproductive behavior and succe...
Article
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Introduction Usually, patients with sY84 or sY86 deficiency present with azoospermia, but recent studies have shown that some males with partial AZFa deletions, including sY84 or sY86, exhibit normal fertility. Here, we reported a rare case of AZF deletion in a family, where both father and son exhibited a deletion at the sY86 site in the AZFa regi...
Article
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The amphibian chytrid fungus, Batrachochytrium dendrobatidis (Bd), has been implicated as an agent of acute declines in amphibian populations worldwide. East Asian amphibians have been coexisting with Bd for long periods and thus are considered resistant; among the many is the Japanese tree frog, Dryophytes japonicus. Our study focused Bd infection...
Article
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Multiple mating is a behaviour observed across various polyandrous insect species. It is suggested that, in ladybirds, this strategy of multiple copulations is used to enhance fecundity and fertility through sperm replenishment. Studies on sperm depletion need to correlate fertility with the presence of spermatozoa in the spermatheca. This study in...
Article
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In animals where males engage in multiple matings, sperm depletion can substantially reduce the reproductive success of both sexes. However, little is known about how successive matings affect sperm depletion, fertilization rates and mating behaviour. Here, we investigated this phenomenon under laboratory conditions. Medaka (Oryzias latipes), an ex...
Article
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Infertility affects ≈15% of couples worldwide, with ≈45% of these cases involving male factors. Semen analysis and sperm selection are critical and routine steps in achieving successful assisted reproductive outcomes. Conventional methods, which are widely used in clinics, are manual, subjective, time‐consuming, and simply not sufficient for the hi...
Article
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Objective Several male factors have been reported to play a role in recurrent pregnancy loss (RPL). The aim of this study is to explore the relationship between semen parameters, sperm DNA fragmentation index (DFI) and RPL. Method A total of 1485 participants were recruited from a university hospital between April 2020 and August 2022. Six hundred...
Article
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Infertility affects around 8%–12% of reproductive-aged couples and is a major health concern. Both genetic and environmental factors influence male infertility. SEPTIN12 is a crucial testis-specific gene essential for the final differentiation of male germ cells and is strongly linked to male infertility due to numerous detected mutations. The pres...
Article
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Arabidopsis MYB transcription factor, AtDUO1 regulates generative cell body (GC) morphogenesis from round to semi and fully elongated forms before pollen mitosis-II (PM II). It was hypothesised that DUO1 might regulate morphogenesis through any of its direct target genes or components of the DUO1-DAZ1 network. The developmental analysis of plants h...
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Missing pixel imputation is a critical task in image processing, where the presence of high percentages of missing pixels can significantly degrade the performance of downstream tasks such as image segmentation and object detection. This paper introduces a novel approach for missing pixel imputation based on Generative Adversarial Networks (GANs)....
Article
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During copulation male insects transfer sperm and seminal fluids, including accessory gland proteins (Acps) to females, produced in the accessory glands (AGs). These Acps influence female behavior and physiology, inhibiting sexual receptivity, promoting ovulation and/or oviposition. The theory of ejaculate allocation postulates that production is c...
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Maximizing the life-long reproductive output would lead to the prediction that short-lived and fast aging species would undergo no – if any – reproductive senescence. Turquoise killifish ( Nothobranchius furzeri ) are naturally short-lived teleosts, and undergo extensive somatic aging, characterized by molecular, cellular, and organ dysfunction fol...
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A Wireless Sensor Network (WSN) is a significant technological advancement that might contribute to the industrial revolution. The sensor nodes that are part of WSNs are battery-powered. Energy is the most crucial resource for WSNs since batteries cannot be changed or refilled. Since WSNs are a finite resource, several techniques have been devised...