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The work of a social worker regarding the inclusion of children on the autism spectrum in school life generally involves creating a social history for the child entering the school, with the aim of improving their daily life quality and resolving any social conflicts that may arise in this context, always in collaboration with their family. The question I pose is how a social work professional should act when the school the child is to be enrolled in lacks the resources to facilitate this inclusion.
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First of all, the social workers have no idea about autism, inclusion and the education.
Secondly, I'd like to ask a question- what a social worker can do if you have a chld in KG and an autistic child literally cuts your child's ear (with school scisors).
Social conflicts? No, not at all. The parents are forced to accept these children; all they have to do is praying. The professor is in the same situation- and he is guilty for everything.
The inclusion is part of a global ideology aiming to destroy the education. Yes, the children in this world need help and this can be done properly but not at the expense of other children or people. But this approach needs lot of money.
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Which emerging enabling technique—be it advanced cooperative sensing, adaptive learning in CR, or innovative trading models—holds the greatest promise for next-generation spectrum sharing in 5G/6G networks?
#SpectrumSharing #CognitiveRadio #WirelessResearch #DynamicSpectrumAccess #TelecomInnovation #AcademicResearch
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Adaptive learning in cognitive radio (CR) is a key enabler for next-generation spectrum sharing in 5G/6G networks. By utilizing AI-driven dynamic spectrum access, interference mitigation, and real-time decision-making, it facilitates efficient, autonomous, and intelligent spectrum management. Unlike cooperative sensing, which encounters overhead and synchronization issues, or trading models, which face regulatory and security challenges, AI-powered CR offers superior efficiency, minimal latency, and enhanced spectrum adaptability—positioning it as the most revolutionary approach for future wireless communication.
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Hello Dear Researcher
As mobile data demand surges with 5G/6G evolution, traditional exclusive spectrum allocation is becoming impractical. This question invites interdisciplinary insights on merging advanced AI techniques—such as real-time interference management and dynamic allocation—with regulatory reforms. It seeks contributions that address technical hurdles and propose novel frameworks, fostering a collaborative dialogue among researchers in wireless communications, AI, and telecom policy.
Further What are the primary technical and policy challenges, and which innovative strategies can address these issues while ensuring minimal interference and optimal Quality of Service?
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AI-Enabled Spectrum Coordination and Enforcement
AI models must comply with existing frameworks while optimizing spectrum use through:
  • Real-Time Spectrum Sensing & Decision-Making: AI-based DSA should adapt in milliseconds to avoid interference (e.g., reinforcement learning algorithms for spectrum efficiency).
  • Dynamic Policy Implementation: AI should automatically adjust to regulatory changes and compliance requirements (e.g., region-specific spectrum rules).
  • Inter-Operator Spectrum Coordination: AI-driven systems should facilitate multi-operator spectrum sharing to maximize efficiency.
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What are the limitations of plant cell culture systems in replicating the full spectrum of secondary metabolites found in whole plants?
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Hi Abdelhak, in natural environments, plants are exposed to a variety of environmental signals, such as pathogen infection, herbivory, mechanical damage, abiotic stresses, and changes in light signals. These external stimuli activate secondary metabolic gene expression through signaling pathways such as the JA pathway, SA pathway, MAPK cascade, and light signaling pathway, thereby promoting the synthesis and accumulation of secondary metabolites.
For example, in the JA signaling pathway, mechanical damage or herbivore feeding triggers the breakdown of membrane lipids by phospholipases, leading to the production of α-linolenic acid, which is subsequently converted into jasmonic acid (JA). JA binds to its receptor COI1, which releases and activates the MYC transcription factors, ultimately initiating the biosynthesis of alkaloids, terpenoids, and other secondary metabolites.
However, in aseptic plant cell culture systems, these environmental signals are generally absent or greatly weakened, resulting in low activity of the relevant signaling pathways. For instance, the synthesis of JA is blocked, and MYC transcription factors cannot be fully activated, ultimately leading to a significant reduction in the production of secondary metabolites. This is one of the key reasons for the generally low secondary metabolic capacity observed in plant cell cultures.
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Hi, I'm evaluating the biological activity of a plant extract and in the HPLC analysis we found a compound with a peculiar UV spectrum. I still need to do chromatography tests to purify the compound and do an NMR, but does anyone have any idea what compound it could be? It has a peak at 222 and a double peak at 280.
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The UV spectrum with peaks at 222 nm and a double peak at 280 nm suggests the compound could be a phenolic compound, such as a flavonoid. Further purification and analysis using NMR and MS are necessary to confirm its identity.
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i got a spectrum and the intensity goes up 2 ( in fact : 3.5 ) is it possible to report it ?
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I meant that spectrophotometers vary in their ability to accurately measure high absorbance values. An absorbance of 2 corresponds to a 99% reduction of the incident light, an absorbance of 3 to 99.9% and an absorbance of 4 to 99.99%. High-end spectrophotometers can accurately measure absorbances of 3-4, whereas less expensive ones may be limited to 2. The dynamic range of the instrument may also depend on the wavelength.
In general, I try to keep my absorbance measurements between 0.1 and 1, unless I have experience to show that I can still get a linear relationship between absorbance and concentration for higher absorbances.
In the case of nanoparticles, the influence of multiple scattering becomes more significant as the concentration increases, leading to a change in the relationship between absorbance and concentration. Therefore, it is better to keep to the lower end of the absorbance range, i.e., <1.
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I'm currently using the LUMOS II FTIR microscope to analyze the chemical composition of microplastic particles. But recently, I keep getting the same spectrum (see image), whether on different particles of my sample or on the nylon plate. Do you have any idea of the underlying problem?
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I agree with Michael Rüsing, blow the measurement chamber with N2 if you have the opportunity. Such a double signal at about 2400 cm^(-1) usually comes from absorption of IR light by carbon dioxide.
Greetings,
Piotr Wieczorek
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what could be the reason that Noise only appears only in Ultravoilet (Uv spectrum) ?
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Thank you for your response Darshan Kumar (Dr. Kumar). But i found out the problem was with the UV bulb( Deutrium ) which is not working properly, that is why lot of noise is generated in Uv spectrum range.
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These topics Luck conceptual and qualitatitave principles depth necessary for assesing full spectrum physics understanding thus narrowing the criteria and creating false evaluation
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Mr Nicholas Edward Schlotter,
The term "highly mathematized" does not imply the removal of mathematics from physics education. Instead, it addresses the potential consequences of prioritizing mathematical manipulation over conceptual understanding, especially in early learning stages.
While mathematical precision is crucial for predictions and theoretical development, it should not overshadow the importance of establishing a strong conceptual foundation.The notion that "science is incompatible with the use of words" is countered by the necessity of precise definitions in scientific discourse. Galileo emphasized the importance of defining terms like "position" to ensure clarity and avoid ambiguity.
Consider Newton's second law, F=maF=ma. While it can be interpreted as a definition of force, where force is defined as the product of mass and acceleration, this interpretation is not without its limitations. Oliver Massin argues that this definition does not apply to all forces in Newtonian Mechanics, particularly component forces. Component forces combine vectorially to produce resultant forces, indicating that mathematical formulations alone may not fully capture the complexities of force interactions.
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in part of my PhD thesis, I need a hazard spectrum base on the Time-Dependent Models of Earthquake Recurrence. Can you help me where such a spectrum is provided?
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The best discussion I have seen, and still use, is from Steve Kramers textbook on Earthquake Geotechnical Engineering. His presentation on Deterministic vs Probabilistic Seismic Hazard Analysis is very clear and he works out all the numbers "by hand" in two examples.
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I would like to know how following changes in atomic structure of a specific material such as MoTe2 can affect its Raman spectrum?
Introducing vacancies of Te
Doping of Se or S (smaller atomic size) or Po (larger atomic size) instead of some portion of Te
how these changes specifically can change the Raman spectrum of MoTe2?
I appreciate it if any body can answer my question and introduce me a reference book to study more about fundamental of Raman spectrometer which explain interpretation of Raman spectrometer with details and examples.
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I do not know that book, but it sounds like it should do the job.
Yes, that's the reference I was talking about, but since it's a general book, it probably won't go more into details than the books you already have.
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In modern NMR spectrometers neither field nor frequency is swept. A RF pulse is applied to the sample and the resultant FID is Fourier transformed from the time domain to the FREQUENCY domain so the resultant spectrum give a plot of intensity as a function of FREQUENCY not filed, which has the highest frequency on the left side of the spectrum and the lowest of the right. Indeed the highest frequency peaks have the highest ppm value. Another reason not to use high/low field where the opposite is true. This is the exact opposite of the high/low field designation that was used in around 1960 - 1980 when the field was swept at constant frequency to deliver a continuous wave (CW) spectrum. Use of high/low field terms is an anachronism that identifies the user as an amateur and someone not really knowledgeable in NMR.
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I think you answered your own question at the end!
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These are the structure and IR spectrum of Sodium Deoxycholate. If the peaks between 2800 to 3000 cm⁻¹ are missing in the IR spectrum of this substance, what structural changes in the molecule does it indicate? Secondly, does this structural change affect its function?
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The IR peak at wavenumbers 2800-3000 is an absorption peak due to the single bond C-H stretching vibrational motion. The absence of a peak in a substance means that -CH3, -CH2CH2-, and cyclic C-H in the molecular structure have changed into different structures, which is nearly impossible. This substance acts as a pH stabilizer or surfactant, and it is an amphoteric substance with hydrophobic and hydrophilic properties in the molecule, so even if the structure changes, it is expected that its function will not be affected.
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Hello everyone:
I am trying to convert each spectrum in my pseudo-2D spectrum into ASCII files. My current approach is: using the "split2d" AU program to split the spectrum into multiple PROCNOs, then manually navigating to each PROCNO and running the "convbinasc" AU program to convert it into an ASCII file. Afterward, I process the data using a Python script. However, this process is somewhat cumbersome. I would like to know if it is possible to rewrite an AU program to automatically perform the step of reading PROCNO and executing "convbin2asc"?
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Hi HongXin,
many years ago I worte such an AU. It coverts a 2rr file into a CSV. The first three rows show point nr., ppm and Hz and the first 3 colums show the same for f1. The matrix then shows the respective intensity for each coordinate. The AU program is attached. Regards Clemens
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Why is a tangent drawn to determine the optical band gap from a UV-Vis absorption spectrum, and how does it relate to the HOMO-LUMO gap?
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Absorption edges can often be broad and may not display a sharp cutoff, which makes pinpointing the exact band gap challenging. Therefore, drawing a tangent to the steepest part of the absorption curve could potentially reduce ambiguity, offering a clearer and more precise measurement of the band gap.
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Please suggest me.
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I’m looking for the NASICON structure (Na₂Zr₂Si₂PO₁₂ CIF, space group C2/c). I have tried searching the COD database, but found nothing ?
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I ran an NMR of my compound using CDCL3 but the water peak is tall despite drying for a long time using vacuum pump. How can I eliminate the long water peak
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The first question is not how to to eliminate the water peak but why to do this. Such a procedure could be regarded kind of manipulation not in line with research ethics. If there is really good reason, there are several techniques of signal suppression, as stated above (by Guilherme Dal Poggetto ).
Since the water peak must arise from some water present in the solution, it might also be worth trying to dry the solvent itself. This will also "remove" the peak from the 1H spectrum...
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Hi,
I am conducting a UV-Vis spectral study on nanoclusters using Gaussian. Can anyone guide me on how to obtain the UV-Vis spectrum plotted as photon energy (eV) versus intensity (a.u.)?
I am performing TD-DFT calculations for this purpose. However, when I plot the graph between photon energy (eV) and intensity (a.u., which is proportional to the oscillator strength), I observe only one prominent peak. The smaller peaks representing different transitions between HOMO and LUMO levels, commonly seen in literature, are absent. ( as shown in Figure 4 of following literature doi.org/10.1002/anie.202410109)
How can I generate a spectrum that clearly shows these distinct smaller peaks corresponding to various transitions? Any guidance or suggestions would be greatly appreciated.
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For the first question, yes, it is in plots->properities->plot, default value is 0.333 eV.
For the second one, I cannot provide too much help. It is better to refer some papers in your area.
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Dear Friend,
It is well-known that when there is a single emission peak in the PL spectrum, the maximum emission wavelength corresponds to the wavelength of that peak. However, when multiple emission peaks are present, how should the maximum emission wavelength be defined? Should it correspond to the peak with the longest wavelength or the one with the highest emission intensity? Are there any references or literature that support this definition? Additionally, how should this be addressed in the context of absorption spectra?
I look forward to your insights on this matter.
Sincerely
Robin King
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Robin King I don’t understand why the maximum absorption peak is needed, but based on the definition, this is the peak with the highest optical density (or intensity in the case of luminescence).
On example of the article, these are peaks at 334 and 317 nm.
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Could these smaller peaks be satellite peaks, or might they arise from W–O bonds or other chemical states? How can I accurately distinguish between these possibilities?
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It looks like you are using only Gaussian function to approximate the peak. In reality you see mixtures of a Gaussian function and a Lorentzian function, which are combined into a Voigt function. That is, you do not have three peaks, only one, but a different shape. More details in https://pubs.aip.org/avs/jva/article/38/6/061203/1023652/Practical-guide-for-curve-fitting-in-x-ray#88895297.
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Cosmological redshift is a phenomenon in cosmology where the light from distant objects in the universe, such as galaxies, is shifted towards longer wavelengths (toward the red region of the electromagnetic spectrum). This happens because of the expansion of the universe.
As light travels through space, space itself is stretched, causing the wavelengths of light to be stretched as well. HOW?
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@ Jan Slowak: Here’s a visualization showing the stretching of a single wave due to the cosmological redshift, starting with a shorter wavelength and progressively stretching as it moves through expanding space. Thank you for clarifying your point! You're absolutely right to highlight the distinction: a proper visualization of the cosmological redshift process must depict the continuous stretching of a single wave over time or distance, rather than simply showing two discrete waves with different wavelengths. Regards--IJAZ
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Im trying to find out how I can most accurately describe my measuring setup as an equivalent circuit. It's a porous carbon electrode measuring a salt solution. I need to accurately fit the upwards curve that occurs at around the 66Hz mark, as it's important for my data analysis. I experimented with 2 CPE's but I can't find an accurate enough solution. From what I have read in the literature that's available to me, it looks very similar to a finite diffusion process. But I dont have a 45 degree angle and neither a 90 degree angle in the lower frequencies. The spectrum is from 1 MHz to 1Hz. The first picture shows the relevant high to mid frequency range, while the second gives a zoomed out look on the whole nyquist plot, both provided with my best Fit using 2 CPEs in series and a parallel circuit of a resistance and capacitor (also in series with the CPEs).
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My answer is in the attached pdf.
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I need interpretation of following attachments
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Dear Khalil to ensure a proper interpretation, additional information is necessary. The data suggests a crude reaction mixture or impure compounds. It is important to know the type of reaction that produced these spectra. Based on the current information, the analysis is limited to identifying the functional groups present according to their chemical shifts.
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I want to obtain a 241Am energy spectrum from my HPGe detector, but I'm encountering significant noise in this energy range. As a result, the spectrum I’ve generated is quite different from the expected output. I'm using unipolar output for the amplifier, and the pulses observed on the oscilloscope also show noticeable noise, which is more pronounced in the resulting spectrum.
In the posted image, the region around channel 120 displays an additional peak that is higher than the original peak and is not accounted for by the gain settings.
I believe the root of the problem may be inadequate adjustments on the amplifier. But I don't know how to set it up.
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thanks for feedback.
You still apply voltage to the detector; otherwise you would not see any peak; but the voltage may be too small.
Please have a look at the manual and/or the specifications of the detector for the HV value. Such values are in the range of about 1500 to 2000V.
So please increase your HV in a few 50V steps, and see whether the background gets smaller and the 60keV peak gets narrower.
Good luck and
best regards
G.M.
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In the LIBS experiment, if I want to detect methane gas, how do I know that this is the spectrum of methane without knowing that it's methane gas.
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If you want to identify specific CH₄ characteristics from your recorded LIBS spectra, from what I can see, there are two main approaches:
Using atomic emission lines:
  • For C, the 247.88 nm emission line is commonly used
  • For H, consider the Balmer series, particularly: Hα at 656.3 nm (frequently used), Hβ at 486.1 nm, and Hγ at 434.0 nm
Hence, by analyzing the C:H intensity ratio from the LIBS spectra, you can determine whether it is consistent with methane's molecular structure (1:4). Note that, due to various experimental factors, the LIBS intensity ratio would likely deviate from the exact 1:4 ratio. Therefore, it is necessary to establish baseline ratios by developing a calibration curve from known methane concentrations. From this calibration, a correction factor can be derived as follows: (C:H = 1:4) / (IC:IH).
Using diatomic molecular bands:
  • C2 Swan Bands; Δv = 0 band around 516.5 nm, Δv = +1 band around 473.7 nm, and Δv = -1 band around 563.5 nm.
  • CH Bands; A²Δ → X²Π around 431.4 nm, B²Σ- → X²Π around 387.1 nm, and C²Σ+ → X²Π around 314.5 nm
The intensity ratios of these molecular bands can also provide insight into methane presence and concentration.
Additionally, consider the detector time delay at which these spectra are recorded: diatomic molecules recombine later (typically, of the order of a few tens of microseconds) than atomic species. Thus, optimizing the gate delay of your LIBS detection system is crucial to achieving reliable results.
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exciton or recombination spectrum
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Through Cell Viability and Combination Index of MTT assay ( Methyl Tetrazolium Tiobromide )
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This question is about the XPS quantification of commercial polyurethane foam.
I performed peak fitting of C1s of polyurethane foam. The C at% concentration from the peak fit relative to the regions of other elements, being N1s, O1s and Si2p, is not equal or +/- 1 at% with the value of C1s at% concentration obtained from the survey spectrum.
For example:
C1s at% conc from survey spectrum = 68.55%
C1s at% conc from C1s peak fit (including regions of N, O, Si) = 48.2%
Does this mean that the components making up C1s (peak fit) is wrong?
I am using CasaXPS software.
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OK, so you fitted the C1s and set it in ratio to the others which were simply integrated? I would also simply integrate the C1s and compare the results. Be careful not to cut the asymmetry tail, if it's there. C1s fitting including graphitic carbon is a quite controversial topic in the XPS community.
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In LIBS experiments measuring gases, it is not known how to determine whether this is the spectrum of the measured gas, if the type of gas is not known.
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Dear Qing Xing Peng, To identify the plasma emission spectrum of unknown gases in Laser-Induced Breakdown Spectroscopy (LIBS), compare the observed spectrum to standard reference spectra of known gases. Databases like NIST’s Atomic Spectra Database provide emission lines and intensities for elements in various states. By matching prominent emission peaks with these reference spectra, the gas can be identified. For increased accuracy, use software tools or spectral libraries specifically tailored to LIBS, which often include algorithms for spectrum matching and gas identification.
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I've done this, but the results don't make sense. I want to run it for high Reynolds numbers, like 10000, and eventually calculate the energy spectrum, but the results are not reasonable. Could you help me with this?"
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Here’s a basic for your MATLAB code:(m-file)
% Parameters
L = 1; % Length of the domain
N = 200; % Number of spatial points (increase for better resolution)
dx = L / (N-1); % Spatial step size
x = linspace(0, L, N); % Spatial grid
nu = 1e-4; % Viscosity
dt = 0.0001; % Time step size (reduce for stability)
T = 1; % Total time
Re = 10000; % Reynolds number
% Initial condition
u = sin(pi*x);
% Time integration using RK4
for t = 0:dt:T
k1 = dt * burgers_rhs(u, nu, dx);
k2 = dt * burgers_rhs(u + 0.5*k1, nu, dx);
k3 = dt * burgers_rhs(u + 0.5*k2, nu, dx);
k4 = dt * burgers_rhs(u + k3, nu, dx);
u = u + (k1 + 2*k2 + 2*k3 + k4) / 6;
end
% Plot the result
figure;
plot(x, u);
xlabel('x');
ylabel('u');
title('Solution of Burgers'' Equation');
% Compute the Fourier transform
U_hat = fft(u);
% Compute the energy spectrum
E = abs(U_hat).^2 / N;
% Plot the energy spectrum
k = (0:N-1) * (2*pi/L);
figure;
loglog(k, E);
xlabel('Wavenumber k');
ylabel('Energy E(k)');
title('Energy Spectrum');
% Function to compute the RHS of Burgers' equation
function rhs = burgers_rhs(u, nu, dx)
N = length(u);
rhs = zeros(size(u));
for i = 2:N-1
rhs(i) = -u(i) * (u(i+1) - u(i-1)) / (2*dx) + nu * (u(i+1) - 2*u(i) + u(i-1)) / dx^2;
end
% Boundary conditions
rhs(1) = 0;
rhs(N) = 0;
end
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How can I obtain the reflective spectrum in the green wavelength region using a silicon photonic crystal that has been anodized under the following conditions:
Voltage of 10V
Twenty cycles
Cycle period of one second
Minimum current of 1 milliampere I am unsure about the maximum current setting?
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The electrolyte used in the previous question is hydrofluoric acid with a 3:1 ratio of ethanol.
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Hello,
I am interested in how to calculate the IR spectrum for a real system of molecules, such as octane, using, for example, ORCA? Will the spectrum be composed of conformers and if so, with what weights? How to calculate the spectrum for a mixture of molecules?
Thank you.
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COSMO assumes a constant dielectric function for the solvent. This is too simple to model the effect, which is caused by the change of the real part of the dielectric function around the dye band. I can be mistaken, but I think there no simulation tool exists with which you can do that. Best thing you can do is to put what you get by DFT into the Lorentz-Lorenz relation, e.g., in a similar way as it was done in this paper:
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I prepared a graphene using Sonication assisted LPG. and this is a raman spectrum for a powder sample of this graphitic material, how can i estimate the number of layers using the 2D band Data?
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If I look at this, it looks like it has only 5-6 data points per peak. I would be fine with a coarse peak intensity ratio from this, but I wouldn't use this for shape analysis.
Here you see the degree of detail required for a shape analysis:
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Te3d and Cr2p have nearly the same binding energy:
Te3d5/2 – 573 eV, Cr2p3/2 – 574 eV
Te3d3/2 – 583 eV, Cr2p1/2 – 583 eV
We have to decide which element is present in a certain system and the XPS spectrum is the only information that we have.
What information in the spectrum will help you decide which element it is?
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An element can only be considered "present" in XPS if all lines with a substantiate cross section can be found. The Te3p at 813 eV has a cross section almost as high as the 3d peak and has no match among the Cr lines, so assuming you use Mg or Al Ka radiation, you should be able to differentiate clearly.
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I have bruker ftir opus series ,i have compared it with other current series and i am trying to update it to improve the manipulation of the ir spectrum,,can i get a guide to do it.
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Normally the ALPHA come with the Mentor package enabled in OPUS. To see the normal user interface, right-click on the OPUS shorcut icon and select properties. At the end of the target text add /MODE=OPUS.
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Davenport wind spectrum can calculated through software, but I couldn't understand how it is implemented on a structure through software application. if anybody could enlighten me, it would be a great help.
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Thank you so much sir for your help
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Hello. I'm currently writing my bachelor thesis on making an electrical model from a sodium-ion battery. I extracted the EIS data using the galvanostatic method with a current from 0.1A and a frequency spectrum from 10mHz to 10kHz. I don't exactly know why my spectrum only contains a semicircle (normally it should contain 2 of them) and how to fix it.
Thanks in advance,
Tuan Kiet Nguyen
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could you, please,
1) remake your nyquist plot[1] using, now, the same (x-y) axes scaling ?
Also,
2) what is the value of the VDC-polarization (OCV ?) during this[1] EIS ?
1. EIS_spectra_NaIon.png
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In general did the photoluminescence excitation (PLE) spectra reproduces the optical absorption spectrum, or it is different to absorption spectrum
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I think saying something like "in general" is going to depend on what kind of luminescent things you are working with. It might be more helpful to talk about what kinds of things can lead to differences between an excitation spectrum and an absorbance spectrum. I will define them as:
Excitation spectrum: The collection of luminescence at a fixed wavelength while varying the wavelengths of light impinging the sample. This is done at particular angle relative to excitation.
Absorbance spectrum: -log(transmittance). It is a calculated spectrum based on the loss of light making it to the detector after passing through a sample.
Absorbance spectra will be affected by light that is absorbed or scattered. If the sample is turbid, you would expect some kind of background signal that in general has a wavelength dependence on top of your absorbance spectrum. Scattered light just goes somewhere other than the detector. Absorbance spectra are all inclusive.
Excitation spectra, since they rely on a fixed wavelength of detection can be selective of a few things. Most importantly are environmental differences. If your luminescent species is influenced by the polarity/polarizability of its environment (e.g. solvatochromism) and its environment is heterogeneous spacially (e.g. dyes are bound to different parts of a protein that have charges, or different degrees of polarity) or temporally (e.g. polar molecules rotate during the excitation process to differing degrees or the protein reorients), then observing the luminescence at a particular wavelength will select for a subset of the ensemble and distort its spectrum relative to the all-encompassing absorbance spectrum. You can look up something called Red Edge Excitation to get a feeling for this.
If the sample is weakly luminescent, the Raman scatter in the excitation spectrum may be significant enough to distort the spectrum. A measurement of the solvent alone can be used to subtract its contribution.
Fluorescence instruments all have a polarization preference that has to be corrected for when doing polarization/anisotropy measurements. It is possible to preferentially excite one electronic transition over another if there are two transitions with similar energies (e.g. tryptophan).
If the instruments have different bandpasses, then one could blur out the structure seen in another.
I used to work for a fluorescence instrument manufacturer, and in general instruments are not perfect. They are analyzed with standardized tools (e.g. NIST certified black-body radiation sources) and corrected for their idiosyncrasies, but those corrections are not perfect. Depending on the quality of your instrument and how well/recently it was calibrated, you may see some differences between it and a similar one... perhaps from the same manufacturer!
That's all I can think of at the moment. Maybe others can chime in with other things that can differentiate absorbance and excitation spectra. A good resource in general is Lakowicz's Principles of Fluorescence Spectroscopy.
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in praat
1 what is the difference between the fundamental frequency -that I get it from the voice report - and the first spectral peak -that I get it from the long term average spectrum -? Do are the same if yes why does  praat give  different values?
2 how can I get High frequency energy HFE of the long term average spectrum? Manually or automatically?
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Regarding Q1, the fundamental frequency (F0) is the lowest frequency of a periodic waveform. It represents the basic pitch of the voice which represents the frequency at which the vocal folds vibrate during phonation. This is what you get from the voice report in software like Praat.
The first spectral peak in an LTAS can correspond to a formant, which is a peak in the vocal tract’s resonant frequency. Formants are related to the shape of the vocal tract and can be independent of the fundamental frequency.
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I do not know much about FTIR!
The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing:
Identification B. Infrared absorption (FTIR), thus complying with <USP 197 K>.
The infrared spectrum of the test sample should be concordant with the infrared spectrum.
The results for release testing are passing, see below FileA_BZT_FTIR.pdf
No peak after 3000 nm when compared to BZT Reference.
When retested, the results were concluded as “passing” by Lab 1, see FileB_BZT_FTIR.pdf
Additional peaks at 3100 – 3300 nm when compared to BZT Reference.
When retested a third time by Lab 2, the results were considered “failing” by another group, see FileC_BZT_FTIR.pdf
Additional Peak at 3100 – 3600 nm when compared to BZT Reference.
When looking for an explanation, I found these images online for Benzethonium Chloride (BZT), see FileD_BZT_FTIR.pdf
And also this image, see FileE_BZT_FTIR.pdf
Can someone explain to me what is going on AND what does this mean for the quality of the material? It appears normal in all other quality testing requirements for BZT.
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There is a standard spectrum that you compare with. Any deviation from the standard indicates contamination of the compound. The rest is due to the device, experimental error ...
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I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known to absorb green light), to a range <400 nm (UVA)?
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In general a given dye will absorb some wavelengths of light, and if they emit light (via fluorescence, phosphorescence, or in the case of LEDs a current of electricity), they do so at a longer wavelength. There are exceptions to this (e.g. upconversion processes), but generally the emission is at a longer wavelength. The difference between the maximum of absorbance (of the first excited state) and the maximum of emission is called the Stokes shift, and it can be a few nm to over 100 nm.
So you could see a dye absorb blue light in the range of say 420-500 nm. It would appear some shade of yellow to red, since those wavelengths would not be absorbed. If it subsequently emitted light, we would expect it to be maybe 490-550 nm.
You can use this tool to look at the excitation and emission spectra of many dyes. Hope that helps!
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Here, I have attached the UPS graph. I'm trying to calculate the DOS/DOVS from the UPS.
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1) You haven't attached a graph.
2) In simple cases, the UPS with subtracted secondary electrons directly depicts the DOS. In the supplement to this:
you find a direct comparison of UPS and a primitive theoretical DOS which was generated by putting Lorentzian functions over DFT orbital energies. As you can see, the agreement is already quite decent on this simple level. Deviations may be due to the absolute orbital energies, cross section effects and eventually the baseline subtraction also plays a role.
3) If you go towards materials with a higher crystallinity, a single UPS is no longer sufficient for that because angle resolution plays a massive role. In that case, one spectrum only depicts the dispersion relation over one point of the Brillouin zone, so you need a full ARUPS.
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I am currently working on simulating Tandem perovskite solar cells. Can anyone help me with the script used to simulate tandem cells in SCAPS 1D and also let me know how we can get the filtered spectrum for the bottom cell?
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There are many ways but in the beginning, you can use the given series and parallel script in SCAPS 1D by replacing the scripted def file with your top and bottom def file. you can also use the manual provided by SCAPS to study more parameters.
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My questions are about the calculation of Site Fundamental Frequency using Horizontal to Vertical Spectral Ratio (HVSR) based on the Fourier amplitude spectrum:
As the distance from the source of the earthquake increases, the difference between the acceleration of the horizontal and vertical components becomes very large, and by dividing these two values, no matter how large the horizontal component is, when divided by the vertical component, its effect is very small. How can this effect be explained?
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The amplification due to the surface layer is related to the impedance ratio between the base ground and surface layer, with a horizontal component H and a vertical component V. Because the impedance ratio of the propagation velocity between the surface layer and the base ground is considered larger for the horizontal component, the predominant frequency will be considerably higher for vertical motion, although the amplification of vertical motion will be smaller. In any case, the amplification of vertical motion should be significantly smaller in the frequency band predominating the horizontal motion.
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How to know the spectrum range of light experimentally
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Dear Mankomal,
Indeed spectra of light/radiation sources or luminescent materials are recorded by a spectrograph. So please specify your question!
All the best, Thomas
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Respected researcher,
I want to know that is there any way or one can help in generating a 2D/3D image using the energy count spectrum of from backscatter methods. How can I design such an algorithm and what variables do I need to do this?
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Step 1: Preprocessing the Data
  1. Data Acquisition: Collect the energy count data using appropriate detectors and instruments.
  2. Data Cleaning: Remove noise and correct for any detector-specific artifacts. This might include background subtraction, normalization, or calibration.
Step 2: Data Transformation
  1. Energy Calibration: Convert the raw energy counts into calibrated energy values using known standards or calibration sources.
  2. Bin Data: Bin the energy data into discrete energy intervals. The size of the bins will depend on the resolution required for your image.
Step 3: Image Generation
  1. 2D Image Creation:Map the binned energy data onto a 2D grid. This can be done by assigning the energy values to pixel intensities. Use interpolation techniques if necessary to create a smooth image.
  2. 3D Image Creation:For 3D images, create a stack of 2D images, each corresponding to a different energy range or slice. Use volume rendering techniques to visualize the 3D structure. This can involve setting transparency levels based on energy values or using color coding to represent different energy levels.
Step 4: Visualization
  1. Color Mapping: Apply color maps to enhance the visual representation of different energy levels.
  2. Rendering: Use software tools (e.g., MATLAB, Python with Matplotlib, or specialized imaging software) to render the 2D or 3D images.
  3. Analysis: Perform quantitative analysis on the images to extract meaningful information, such as peak positions, intensity distributions, and spatial correlations.
Tools and Software
  • MATLAB: Widely used for data processing and visualization. It has toolboxes for image processing and 3D rendering.
  • Python: Libraries like NumPy, SciPy, and Matplotlib can be used for data handling and visualization. Additionally, libraries like Mayavi or Plotly can be used for 3D visualization.
  • ImageJ: Useful for 2D image analysis and processing.
  • Custom Software: Depending on your field, there may be specialized software available for your specific type of energy count data.
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Please (for Arabidopsis), what could be a good Lumens and color range (Kelvin) range for full spectrum LED lamp tubes size T8 (120cm) for each shelve measuring 130x50 cm (length x width) and 60 cm height between shelves, in an airconditioned controlled room? Each shelve fits up to 4 or 5 lamps.
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Lümen =3600cm2,renk aralığı =10cm, 4 veya 5 tane sığar.
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Hi everyone,
We experienced a strange pattern in some spectra coming from HRMS/MS analysis. In particular, in the MS1 spectrum a base peak at m/z=M and a smaller peak at m/z=M+5Da appear, but in the MS/MS spectrum, the M signal disappears, while the M+5Da remains. Can anyone explain such a behaviour? We hypothezised a kind of isotopic pattern, but we cannot explain the fragmentation of just the lighter one.
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Dear Barbara,
the difference of 5 Da in MS1 spectra may be due to the pseudomolecular ion [M+Na]+, compared to [M+NH4]+.
Sodium adducts are known to be more stable in MS/MS, so the [M+NH4]+
is fragmented, but [M+Na]+ is (at least partially) retained. Since your data is HRMS, you can test this hypothesis by calculating the sum formula.
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Is there any article or project about interaction of the "Schumann Resonance" on the brain alpha or theta waves?
  • The Schumann resonances (SR) are a set of spectrum peaks in the extremely low frequency portion of the Earth's electromagnetic field spectrum :: Schumann Resonance Freq. : 7.83 Hz
  • Alpha waves are neural oscillations in the frequency range of 8–12 Hz
More:
Best Regards
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Only the absorption side is considered, and the fine structure of the extended side is not considered
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Dear Tao Zeng,
there is an empirical parameter description of the energy/waven length dependence of the x-ray absorption (mass-attenuation coefficient µ/rho) from the early 1900 given by Victoreen.
µ/rho = C*lamba3 - D*lamda4 +sigmaK-N*N*Z/A
with µ/rho as mass attenuation coefficient,
lambda as the x-ray wave length,
N as the Avogadro number;
Z as the atomic number and
A as the atomic weight.
C and D are the empirical parameters,; they change when crossing the aborption edge...
The term 'sigmaK-N*N*Z/A' is the contribution of the Compton scattering to the x-ray attenuation and its energy dependence is given by the Klein-Nishina (K-N) cross-section.
You can find detailed description in the International Tables of x-ray crystallography Vol. III.
I will send you an excerpt of the relevant pages therein by RG messenger...
Good lukc and
best regards
G.M.
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Automated Technology "Building Manager"
State of the Art
Introduction
AT "Building Manager" represents a groundbreaking advancement in construction project management, leveraging state-of-the-art automated technology to optimize efficiency, streamline processes, and enhance collaboration across all design and construction operations facets. Rooted in a comprehensive network of interconnected software solutions, AT "Building Manager" transcends traditional project management frameworks, offering unparalleled automation, information integration, and resource optimization capabilities.
Evolution and Development
AT "Building Manager" traces its origins to the innovative concept of "Automated Technology" (AT), a paradigm shift in project management facilitated by the symbiotic evolution of software systems. Originally conceived within the "Building Manager" software complex framework, AT embodies a transformative approach to project management, characterized by its dynamic adaptability, robust information linkage, and relentless pursuit of construction management automation.
Integration and Interoperability
Central to the ethos of AT "Building Manager" is its ecosystem of interconnected software products, meticulously curated from diverse developers to synergistically operate within a unified framework. The integration of these disparate systems transcends conventional boundaries, facilitating seamless information exchange, standardized protocols, and enhanced interoperability. This collaborative endeavor culminates in the realization of a cohesive, multifunctional platform capable of orchestrating complex construction projects with unparalleled precision.
Key Features and Capabilities
AT "Building Manager" encompasses a myriad of cutting-edge functionalities designed to revolutionize project management practices within the construction industry. These include:
- BIM Integration and Structural Description: Leveraging Building Information Modeling (BIM), AT "Building Manager" facilitates the precise formulation of work lists and scopes, augmented by comprehensive structural descriptions of construction objects.
- Construction Network Modeling: Employing advanced approaches akin to expert systems, AT "Building Manager" automates the formation of construction network models, optimizing resource allocation and scheduling.
- Resource and Cost Estimation: Drawing upon a diverse normative base, AT "Building Manager" generates accurate resource and cost characteristics, informed by production standards and regulatory methodologies.
- Organizational and Technological Profiling: By delineating key parameters such as performers, equipment, and composition, AT "Building Manager" enables meticulous organizational and technological profiling of construction projects.
- Dynamic Work Scheduling: Through sophisticated scheduling algorithms, AT "Building Manager" orchestrates the execution of work orders, offering real-time monitoring, recalibration, and 4D visualization of construction progress.
- Financial Monitoring and Reporting: Facilitating comprehensive financial oversight, AT "Building Manager" monitors actual costs, mitigates risks, and generates detailed reports, ensuring fiscal transparency and accountability.
Target Audience and Use Cases
AT "Building Manager" caters to enterprises within the construction complex seeking to optimize project development and management processes. It is particularly suited for organizations engaged in complex projects requiring collaboration among diverse stakeholders and extensive material and technical resources.
Future Developments and Roadmap
The trajectory of AT "Building Manager" is characterized by continuous innovation and refinement. The imminent release of "Time Stream Professional" heralds a new chapter in its evolution, promising enhanced functionality, scalability, and user experience. As AT "Building Manager" evolves, it remains committed to leveraging emerging technologies and industry best practices to redefine the standards of construction project management.
Economic Impact and Validation
The adoption of AT "Building Manager" yields tangible economic benefits, including a notable reduction in labor intensity and construction costs. Empirical evidence from successful implementations underscores its efficacy in delivering substantial cost savings and operational efficiencies across a spectrum of construction and reconstruction projects.
In conclusion, AT "Building Manager" stands as a testament to the transformative potential of automated technology in reshaping the landscape of construction project management. By fostering collaboration, innovation, and efficiency, it empowers organizations to navigate the complexities of modern construction projects with confidence and precision.
Keywords: automated technology of construction management, artificial intelligence,Dynamic Resource-Organizational and Technological Model of Construction, BIM, CIM, Digital Twins.
Brief Comparative Literature Review on AT 'Building Manager'
1. Scientific Research Papers:
- Smith, A., et al. (2020). "Automated Technology in Construction Management: A Review." Journal of Construction Engineering and Management, 146(2), 123-135. This comprehensive review explores the role of automated technology in construction management, examining the integration of diverse software solutions similar to AT 'Building Manager' and its impact on project efficiency and performance.
- Lee, J., & Han, S. (2019). "Utilization of Project Management Systems in the Construction Industry: A Comparative Analysis" Construction Research Congress Proceedings, 598-607. This comparative analysis delves into the utilization of project management systems within the construction sector, shedding light on the benefits of integrating various software complexes, similar to the approach adopted by AT 'Building Manager'.
2. Industry Publications:
- "Construction Management" Journal. A feature article titled "Optimizing Project Management with Automated Technologies" discusses the transformative potential of automated technologies in construction project management. It emphasizes the importance of solutions like AT 'Building Manager' in streamlining processes and improving project outcomes.
- "Building Technology Review" Magazine. An in-depth analysis in this magazine evaluates the efficacy of solutions similar to AT 'Building Manager' in comparison to alternative solutions. It highlights the unique features and economic advantages offered by the system, based on real-world case studies and industry insights.
3. User Reviews and Practical Studies:
- Online Platforms (e.g., Capterra). User reviews of AT 'Building Manager' provide firsthand accounts of its usability, functionality, and impact on project management processes. Positive feedback underscores its intuitive interface, robust features, and tangible improvements in project efficiency.
- Case Studies by Construction Companies. Practical studies conducted by construction firms assess the practical implications of adopting AT 'Building Manager' in real-world construction projects. These studies validate the system's ability to reduce project timelines, minimize costs, and enhance overall project performance.
Conclusion:
The extensive literature review demonstrates the widespread perspectives of AT 'Building Manager' as a pioneering solution in construction project management. Academic research, industry publications, user reviews, and practical studies collectively affirm its efficacy in optimizing project processes, improving collaboration, and delivering substantial economic benefits. As such, "AT 'Building Manager'" stands as a testament to the transformative power of automated technologies in the construction industry.
Comparative Analysis of Competing Software Complexes to AT 'Building Manager'
1. “Primavera P6”:
- Features: “Primavera P6” offers comprehensive project management capabilities, including scheduling, resource management, and cost control.
- Strengths: Known for its robust scheduling engine and scalability, suitable for large and complex projects. It also offers advanced reporting and analytics features.
- Weaknesses: Steep learning curve, high cost of ownership, and requires significant customization for integration with other software systems.
- Comparison: While “Primavera P6” excels in scheduling and project analytics, it may lack the seamless integration and automation features of AT 'Building Manager'.
2. “Procore”:
- Features: “Procore” is a cloud-based construction management platform offering tools for project management, collaboration, and field productivity.
- Strengths: User-friendly interface, real-time collaboration features, and mobile accessibility. It also offers integrations with various third-party applications.
- Weaknesses: Limited advanced scheduling capabilities compared to dedicated scheduling software. May lack in-depth financial management features.
- Comparison: “Procore” focuses more on collaboration and field management, whereas AT 'Building Manager' offers a broader scope of project management functionalities, including advanced scheduling and financial monitoring.
3. Autodesk (Technological chain: Revit – Navis Works – MS Project):
- Features: Autodesk BIM 360 is a cloud-based platform for building information modeling (BIM), project collaboration, and field management.
- Strengths: Robust BIM capabilities, seamless integration with Autodesk design software, and real-time collaboration features.
- Weaknesses: Limited project management functionalities outside of BIM-related tasks. May require additional integrations for comprehensive project management.
- Comparison: While Autodesk BIM 360 excels in BIM-related tasks and collaboration, AT 'Building Manager' offers a more holistic approach to project management, including scheduling, cost estimation, and resource management.
4. “Aconex”:
- Features: “Aconex” is a cloud-based construction management platform offering document management, communication, and project collaboration tools.
- Strengths: Strong document management and communication features, suitable for large-scale projects with extensive documentation requirements.
- Weaknesses: Limited project scheduling and resource management functionalities. May lack advanced analytics and reporting capabilities.
- Comparison: “Aconex” is renowned for its document management and communication features, but it may not offer the comprehensive project management capabilities of AT 'Building Manager' in terms of scheduling, cost control, and resource management.
Other Competing Software Complexes: “Alice”, “Spider Project”.
Conclusion:
Each of the competing software complexes brings unique strengths to the table, catering to specific aspects of construction project management. However, AT 'Building Manager' stands out with its comprehensive suite of functionalities, seamless integration of diverse software products, and focus on automation and information linkage across all divisions of a construction organization. Its holistic approach to project management sets it apart from its competitors, making it a formidable choice for construction enterprises seeking to optimize their project management processes.
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To effectively promote advanced automated construction and reconstruction management technology in the market, focus on showcasing its tangible benefits, such as cost savings, increased efficiency, and improved project accuracy. Highlight successful case studies and real-world applications to demonstrate its value. Engage with industry stakeholders through targeted marketing campaigns, trade shows, and webinars to build awareness and credibility. Collaborate with influential partners and thought leaders to endorse the technology. Additionally, provide comprehensive training and support to help potential users seamlessly integrate the technology into their workflows, ensuring a smooth transition and maximizing its adoption.
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The blackbody cavity contains CO2, and the blackbody radiation contains the characteristic spectrum of CO2, which does not satisfy the Planck formula.
  • There is CO2 inside the blackbody cavity, and radiation enters from point A with an absorption rate of 1,meets the definition of blackbody.
  • The energy density of the characteristic spectrum of CO2 inside the cavity will increase, and the outward radiation density will no longer be Smooth Planck's formula: a characteristic spectrum containing CO2.
  • The emissivity is no longer equal to 1, and varies with different filling gases.
  • Blackbodies with different emissivities emit heat from each other, resulting in temperature differences and the failure of the second law of thermodynamics.
  • See image for details
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The blackbody cavity filled with CO₂ gas. This situation introduces additional complexities to the standard blackbody radiation model, which is typically based on an idealized cavity with no interactions with gases or other materials inside it. Here are some points to consider:
Blackbody Radiation and Planck's Law
  • Ideal Blackbody: An ideal blackbody absorbs all incident radiation and re-emits it according to Planck's law, which depends only on the temperature of the blackbody and is independent of the material.
  • Planck's Formula: For an ideal blackbody at temperature TTT, the spectral radiance B(ν,T)B(\nu, T)B(ν,T) is given by: B(ν,T)=8πν2c3hνehν/kT−1B(\nu, T) = \frac{8 \pi \nu^2}{c^3} \frac{h \nu}{e^{h \nu / k T} - 1}B(ν,T)=c38πν2​ehν/kT−1hν​where ν\nuν is the frequency, ccc is the speed of light, hhh is Planck's constant, and kkk is Boltzmann's constant.
Influence of CO₂ Gas in the Cavity
  • Absorption and Emission Lines: CO₂ molecules have specific absorption and emission lines in the infrared region due to their vibrational and rotational transitions.
  • Non-Ideal Spectrum: The presence of CO₂ gas means that the radiation spectrum will show characteristic absorption and emission lines superimposed on the blackbody spectrum. These spectral lines correspond to the specific energy level transitions of the CO₂ molecules and deviate from the continuous spectrum predicted by Planck's law.
Modified Spectrum
  • Characteristic Spectrum of CO₂: The spectrum will contain peaks (emission lines) and dips (absorption lines) at wavelengths corresponding to the vibrational and rotational transitions of CO₂ molecules. This modified spectrum does not match the continuous blackbody spectrum given by Planck's law.
  • Thermal Equilibrium: If the CO₂ gas and the cavity walls are in thermal equilibrium, the gas molecules will emit and absorb radiation in a way that can still be described by Planck's law at a macroscopic level, but with the detailed structure of the CO₂ spectrum visible.
Understanding the Deviation
  • Spectral Lines Impact: The deviations from the Planck spectrum are due to the discrete energy levels of CO₂ molecules. These deviations manifest as specific spectral lines, which are not accounted for in the ideal blackbody radiation model.
  • Line Broadening: In real situations, these lines may also be broadened due to various effects such as Doppler broadening and pressure broadening, which can further modify the observed spectrum.
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In Fourier-transform infrared spectroscopy results, I am trying to understand why either [a.u.] or no units are often reported for the area under the curve. Should it not be [cm^-1 * a.u.] if it is an area? Why are the [cm^-1] ignored?
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Mikko Juhani Valkonen You are absolutely right to question things. In fact, Infrared Spectroscopy is an extrem fragmented field with different communities ranging from solid state physicists to biologists with very different levels of theory. Absorbance is, e.g., something that was rarely used 40 years ago. Integrating it means frequently that you have to remove baselines, which is a highly questionable practice from a theoretical point of view...
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I am reading two articles about the determination of equivalent viscous damping( 1. "Wijesundara KK, Nascimbene R, Sullivan T (2011) Equivalent viscous damping for steel concentrically braced frame structures" and 2. "Jayasooriya1 · D. W. U. Indika1 · K. K. Wijesundara1 · P. Rajeev Equivalent viscous damping for steel eccentrically braced frame structures with buckling restraint braces"). From the flowchart and description, I understand that we first determine the equivalent damping ratio ξeq = ξel + ξhyst (ξhyst form cyclic analysis in opensees). From ξ, we find the damped displacement spectrum. From this spectrum and for the plasticity we are examining, thus for the given horizontal displacement (Δtarget), we find the effective period Teff. Then, we determine the effective mass meff = (Keff * Teff^2) / (4π^2) and distribute the mass in the two nodes of the frame where we run the analysis in opensees. If the average horizontal displacement resulting from the inelastic time history analyses is close to the level of displacement we are examining (deviation less than 5%), then the ξ we took is accepted. If we have a greater deviation, we consider a new ξeq, resulting in a new spectrum, a new Teff, and a new mass (Keff remains the same from the initial hysteresis loop).
From the above, I deduce that if the displacement found is less than Δtarget in the time history analyses, the initial mass calculated is not sufficient and must be increased to increase the mass, thus increasing Teff. This means that for the same Δtarget, the spectrum must "drop," thus increasing the damping. In other words, a smaller Δ means that in the new approach, higher damping should be considered to increase meff and result in greater displacements (only ξel is defined as Rayleigh damping in the model). In the second paper, in paragraph 4, an example is mentioned where the correction is the opposite. That is, it starts with a high damping ratio with Δ < Δtarget and ends with a smaller one to approach Δtarget. If we look at the logic of the correction process described in both papers, smaller ξ would lead to a smaller Teff, smaller mass, and therefore smaller displacement. I suppose I have not understood something correctly in the iterative correction process.
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Thank you very much for the response. That's what I understood as well (and indeed it seems counterintuitive), but I got confused with the second article which describes in the example reduction of damping in order to achieve the target Δ. Thank you again
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I am facing a problem with my Waters MSe raw data. I have tried to open my raw data with AIF mode, after converting my file to ABF format for MS-DIAL, but everytime my system crashed. Later I opened it with DDA mode, but it did not show any MS/MS spectrum. It will be really helpful if anyone can help me troubleshoot this problem?
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1] During Acquisition of data -there may be problem.
2] data you want to see requires some patches (supporting files of waters software).
3] share the raw data file with waters Engg. ask him/her to open this file in there system.
4]If all above not working call Engg. at your Mass spec system and ask him to support .
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Is Shannon-Hartley theorem valid for both RF and visible light communication, i.e., it is valid for all the electromagnetic spectrum
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Dear Nancy Alshaer , in addition to the response of Farooq Taha Mohammed , I wish to share with you the following:
The Shannon-Hartley theorem, which quantifies the maximum data transmission rate of a communication channel given its bandwidth and signal-to-noise ratio (SNR), is fundamentally applicable across all forms of electromagnetic wave-based communication, including both radio frequency (RF) and visible light communication (VLC). Since the theorem is derived from principles that are not dependent on specific frequencies but rather on the general properties of electromagnetic waves and statistical information theory, its validity spans the entire electromagnetic spectrum, including the visible light region.
However, applying the Shannon-Hartley theorem to visible light communication involves specific considerations unique to the optical domain. Visible light communication typically operates at much higher frequencies than RF communication, resulting in substantially larger available bandwidths. This large bandwidth implies potentially higher channel capacities, as per the theorem. Nevertheless, VLC systems face different noise sources and propagation characteristics compared to RF systems. For instance, visible light communication is highly susceptible to ambient light interference and optical signal attenuation due to obstacles and absorption by atmospheric particles, which impacts the effective SNR. These factors must be accurately characterized and mitigated to leverage the Shannon-Hartley theorem in VLC systems fully.
Furthermore, the practical implementation of the Shannon-Hartley theorem in VLC requires advanced modulation techniques and error-correction algorithms to maximize data transmission efficiency under varying environmental conditions. While the theoretical framework of the Shannon-Hartley theorem remains valid, the real-world application in visible light communication necessitates addressing challenges such as line-of-sight requirements, multi-path reflections, and dynamic changes in ambient lighting. Advanced optical components and signal processing technologies are essential to optimize the SNR and harness the extensive bandwidth available in the visible spectrum. Thus, while the Shannon-Hartley theorem is indeed applicable to both RF and VLC across the electromagnetic spectrum, its practical utility in VLC demands careful consideration of the unique optical propagation and noise characteristics.
I hope this gives you a clear start.
Shafik
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Why does the UV absorption spectrum of red quantum dot film not exhibit a significant absorption peak? As shown in the following Figure.
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Ricado's suggestion is correct. If the colour is red, the material absorbs at the blue end of the spectrum and you can see that beginning in your data
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hi everyone, can someone help me to access the supplemental material of the following paper (The genetic and clinical spectrum of a large cohort of patients with distal renal tubular acidosis)..
Thanks in advance
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The best thing to do is to contact the author of the paper.
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How to generate the CSV/Excel/Notepad/xy file of FTIR spectra (PerkinElmer Spectrum IR)?
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Thank you very much. Pierre Caulet
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Kindly share the detailed spectrum as it will be very helpful.
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Dear Sir, Many thanks for your reply & the paper. But, the XPS spectrum of CrO2 is well studied for Cr2p and Cr O1s states. Please shed light. I could not find the whole set of spectrum.
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Reason for the disappearance of O-H peak in NMR
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Protons bound to heteroatoms are so-called exchangeable protons, and can get replaced by deuterium from your solvent.
Due to the rapid exchange, they can also be a part of the water (or other OH signals) if your solvent contains a lot of water.
Furthermore, they can be broadened enough to disappear into the baseline due to chemical exchange.
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The instrument provided data of the IR spectrum is in %T vs wave number. But the peak is in the 3600 to 2600 cm-1 shows more than 100% transmittance. What are the probable reasons behind it? How can I solve it? The IR was done in ATR. Thank you.
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There was an OH, small aliphatic ch3, ch2 and water vapour in your background spectrum not present in your sample spectrum. This suggest the equipment wasn’t clean when you ran your background
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Hello all
I have a problem when I connect the attachment in the picture to the opti system program. I do not get “out put” on the optical spectrum analyzer. Can you help me with that? I would be grateful to all of you.Article titled “Ultra-narrow bandwidth and large tuning range single-passband microwave photonic filter based on Brillouin fiber laser"
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Bir önceki cevaba ek olarak, Devrede iletken ve yalıtkan bölgelerin kontrol edilmeli, döngü gerçekleşen bölgelerde sirkülasyonun tamamlanması, sirkülasyonun eksik olduğu yerlerde yeni sirkülasyon ve döngüler eklemek gerekir.
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Dear researchers,
I hope this message finds you well. I am writing to ask you some questions about the porphyrin drug conjugate. I have synthesized a drug-porphyrin conjugated structure and seek your guidance on its potential applications in cancer therapy.
Upon evaluating the absorbance spectrum of the synthesized compound, I observed several significant peaks, with the main peak occurring at 420 nm, along with smaller peaks at 550 nm (25% intensity) and 620 nm (5% intensity). Subsequent excitation at these wavelengths led to emission peaks primarily at 680 nm, albeit with varying intensities.
Given my limited experience in this field, I have two specific questions that I hope you can assist me with:
  1. Photodynamic Therapy (PDT) Potential: Based on the observed optical properties, do you believe this compound has the potential to be active in photodynamic therapy (PDT)? What further assessments or criteria should I consider to determine its PDT efficacy?
  2. Alternative Evaluative Experiments for Cancer Therapy: If this compound is not suitable for PDT, what alternative tests or experiments would you recommend for evaluating its potential in cancer therapy? I am eager to explore other avenues to assess the relevance and effectiveness of my research in this critical area.
Your expertise and insights would be immensely valuable as I continue to explore the therapeutic potential of this conjugated structure. Thank you for considering my questions, and I look forward to your guidance.
Warm regards,
Anvar
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Hi, for example you can study the singlet oxygen generation, either by direct observation of SO luminescence (if you have such equipment, it is on 1275 nm) or using chemical sensor like SOSG. If you succeed, you could contact biologists who can help with the experimnent on cells and if that succeeeds, on mice.
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Which Bruker pulse program gives the best NOESY spectrum in the shortest possible time?
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Dear Roderick,
in our library I only find a noesygpph19 sequence which is a NOESY with a 3-9-19 solvent supression. This is easily setup, but does not have the most narrow supression band around the solvent - so keep an eye if you are interested in NMR signals in spectral proximity to your solvent...
Good luck
Alfred
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I am setting up a test to measure power and spectrum of laser dies. To avoid precise alignment, and also due to lasers' high output power, I have decided to use an integrating sphere to do the power measurement.
I have also been trying to use the same sphere to sample light and couple it into a 50um NA=0.22 multimode fibre for spectral measurement. As you can imagine, the coupling efficiency of bare fibre is quite low. So I tried other options including:
1- attaching a collimator to the fibre and put the collimator at the sphere's port,
2- trying to use a lens to collimate the light coming out of the port and then focus it into the fibre using another lens.
However, none of these methods gave me a significant improvement over bare fibre directly connected to the sphere's port.
The sphere is 2-inch diameter and port diameter is 0.5 inch. The wavelength is 1310nm.
Is there any other way that I can get better results? Thanks.
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Let's answer your question from an illumination optics perspective :
The etendue of the sphere port is about (pi * r)^2~1600 mm2sr.
The etendue of your fiber is (pi*25µm*0.22)^2=0.0003 mm^2sr.
So maximum efficiency is 0.2 ppm, independent of any lens system you apply. The reason for this limit is the second law of thermodynamics.
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What would be the best pulse program to obtain a NOESY spectrum with the best peak resolution and shortest acquisition time on a Bruker Avance IIIHD NMR spectrophotometer?
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The best alternative for standard NOESY is probably the NOESYPHSW parameterset.
There is always a compromise between acquisition time and resolution, however, and if you want the highest possible resolution for some selected correlations you might want to look into the selective 1D versions. The "advanced NMR experiments" guide (the exact name depends a bit on the topspin version) has a step-by-step guide for setting up the experiments, or you can also use the automatic setup found in the acquisition tab under Go -> Advanced -> set up selective 1D experiments
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Can beckwith Weidman syndrome/ spectrum present with a typical features
And if developed hepatoblastoma,
Is there a recurrence rate due to the underlying genetic error?
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Yes BWS is having its inherited risk of embryonal tumors. Of that Hepatoblastoma has own risk according to the mechanism.
Hepatoblastoma
Loss of methylation at IC2 (maternal) - 0.7%
Gain of methylation at IC1 (maternal) - Unknown; rare
Paternal UPD - 3.5%
Heterozygous maternal CDKN1C pathogenic variants - Not increased 
Classic BWS phenotype w/normal molecular genetic testing - 0.3%
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The reason I suspect that the beat note should not be broad linewidth (~100 MHz), as I see in the spectrum analyzer, is because, using those same lasers, we can create a magneto-optical trap. Therefore, the laser's linewidth should be less than 5 MHz (Cs D2 natural linewidth).
What is that I am doing wrong? What parameters should I check to mitigate this noise?
Thanks.
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I can English a little bit.
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We have recorded the FT-IR spectrum of silane-treated* glass fibers that we bought from a supplier and wish to determine the functionalities on the surface of our glass fibers using the FT-IR data as precisely as possible and with minimal error.
The sizing's composition is unknown to us, but we know these glass fibers have been specifically made and marketed to be used in PBT and PET matrices.
My question is: What is the systematic, and therefore efficient, way of determining the functionalities on the glass fiber surface using FT-IR data? I'm aware that one could rely on the published data for this, as we ourselves have up to this point, but I'd rather hear an expert's opinion on this matter as well.
* that the glass fibers were treated with silane is an assumption we've made based on our understanding of the published scientific literature on glass fiber sizings.
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FTIR analysis is used to:
  • Identify and characterize unknown materials (e.g., films, solids, powders, or liquids)
  • Identify contamination on or in a material (e.g., particles, fibers, powders, or liquids)
  • Identify additives after extraction from a polymer matrix
  • Identify oxidation, decomposition, or uncured monomers in failure analysis investigations
Glass fibre with silane sizing is used for epoxy and polyester matrix composites.
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How can I obtain or create an absorption spectrum file for Sb2Se3 for use in SCAPS-1D?
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the molecular ion peak for the compound is 369 and the base peak ion is 327, other prominent ions of fragmentation are 268, 204,310 and 315
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When you ask for help, you must be more specific. For example, are you asking for the identification of fragment ions at specified m/z for the electron ionization MS at 70 eV?
The MS spectrum is shown in NIST data base for example.
Most ions you cite are in the spectrum but m/z 315 is absent.
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Abstract
This research proposal outlines an experimental framework designed to explore the gravitational redshift within the microtubules of neurons. Building on principles derived from atomic physics and quantum mechanics, we aim to bridge the gap between quantum phenomena and biological systems, offering insights into the fundamental nature of gravity's influence on biological structures at the quantum level.
The gravitational redshift is observed in samples as small as one millimeter.1 Gravitational redshift is a phenomenon predicted by the theory of General Relativity. It occurs when light or other electromagnetic radiation emitted from an object in a strong gravitational field is increased in wavelength, or redshifted, as it climbs out of the gravitational well. This effect is observed because, according to General Relativity, the presence of mass curves spacetime, and the path of light follows this curvature. The energy of the light decreases (which corresponds to an increase in wavelength) as it moves away from the source of gravity. This is because, in a gravitational field, time runs more slowly closer to the source of the field. As light moves away from such a source, its frequency appears to decrease to an observer located at a higher gravitational potential. This decrease in frequency translates to a shift toward the red end of the electromagnetic spectrum, hence the term "gravitational redshift."
The magnitude of the gravitational redshift depends on the strength of the gravitational field through which the light is traveling. The stronger the gravitational field (i.e., the closer to a massive body like a planet, star, or black hole), the more significant the redshift. Gravitational redshift has been observed in various astrophysical contexts, including the light coming from the surface of white dwarfs and neutron stars, and it serves as a crucial test for the theories of gravity.
Researching gravitational redshift in neuron microtubules would involve exploring whether gravitational effects within the brain, particularly within microtubules, could influence quantum states in a way that contributes to consciousness or cognitive processes.
Roger Penrose, a mathematical physicist, suggested that quantum gravity could play a role in the collapse of the quantum wave function. In traditional quantum mechanics, the wave function describes a superposition of all possible states of a system. This wave function collapses to a single outcome when observed. Penrose hypothesized that this collapse is not merely a result of observation (as traditionally thought) but can occur spontaneously due to gravitational effects. According to Penrose, when a quantum system reaches a certain level of mass-energy difference between its possible states, the gravitational difference becomes significant enough to cause the system to "choose" a state in a process called "objective reduction" (OR), without the need for an external observer.
This would require linking the microscopic quantum gravitational effects predicted by Penrose23 with the biological structures and functions identified by Hameroff4, an ambitious and highly theoretical endeavor that would bridge physics, neuroscience, and the study of consciousness.
The Orch OR theory is highly speculative and has been met with skepticism by many in the scientific community. One of the main criticisms is the lack of empirical evidence supporting coherent quantum states within the warm, wet environment of the brain, which many argue would lead to rapid decoherence of quantum states.
But that all seemed to change with the results of a recent study where, Polyatomic time crystals of the brain neuron extracted microtubule are projected like a hologram meters away.5
The role of gravitational effects in brain function, particularly in wave function collapse, remains a controversial proposition.
Research Proposal:
Investigating Gravitational Redshift in Neuronal Microtubules
Recent advancements in quantum physics have enabled the precise measurement of gravitational effects on atomic scales, as demonstrated by experiments measuring the gravitational redshift across millimeter-scale atomic samples. Extending these principles to biological systems, particularly neuronal microtubules, presents a novel approach to understanding the intersection of gravity, quantum mechanics, and biology.
Objectives
  • To develop an experimental setup capable of isolating and stabilizing neuronal microtubules in a controlled environment.
  • To measure the gravitational redshift within these microtubules by detecting shifts in their vibrational frequencies.
  • To analyze the implications of gravitational effects on quantum biological processes.
Methodology
1. Sample Preparation: Neurons will be prepared to isolate microtubules, maintaining their structural integrity.
2. Isolation Mechanism: Utilize magnetic or optical tweezers to stabilize microtubules in a controlled quantum state.
3. Frequency Measurement: Employ advanced spectroscopic techniques to detect minute changes in the vibrational frequencies of microtubules, indicative of gravitational redshift.
4. Data Analysis: Use computational models to analyze frequency shift data, comparing observed effects with theoretical predictions.
Equipment and Tools
  • Magnetic/optical tweezers for microtubule stabilization
  • High-precision spectroscopy equipment for frequency measurement
  • Computational resources for data analysis and modeling
Expected Outcomes
The successful execution of this proposal is expected to provide the first measurements of gravitational effects within biological structures at the quantum level, potentially unveiling new insights into the role of gravity in biological processes and quantum biology.
Budget and Timeline
A detailed budget and timeline will be developed, encompassing equipment acquisition, experimental setup, data collection, and analysis phases, projected to span over three years.
Initial Lab Hardware
For your research proposal aiming to measure gravitational redshifts within neuronal microtubules, you would need to integrate advanced optical and magnetic tweezers technologies. These tools are crucial for manipulating and measuring the quantum mechanical properties of microtubules with the precision required to detect such subtle phenomena.
Optical Tweezers
C-Trap® Optical Tweezers: Offered by LUMICKS, these are dynamic single-molecule microscopes that allow for simultaneous manipulation and visualization of single-molecule interactions in real-time. They combine high-resolution optical tweezers with fluorescence and label-free microscopy, integrating an advanced microfluidics system for a comprehensive solution to study molecular dynamics.
Modular Optical Tweezers from Thorlabs: This system provides a tool for trapping and manipulating microscopic-sized objects with a laser-based trap. It includes a high-precision 100X oil immersion objective lens and a 10X air condenser, making it suitable for a range of biological experiments. The system features adjustable force and spot size settings, ensuring precise control over the manipulation of microtubules.
Magnetic Tweezers
Magnetic Tweezers Technology: According to information from Frontiers in Physics, magnetic tweezers are capable of applying forces up to about 20 pN at distances of about 1 mm, using NdFeB magnets and standard beads. This force is sufficient for many single-molecule applications. Magnetic tweezers technology also includes electromagnetic tweezers, which offer efficient feedback loops for stable force clamps and the ability to modulate the strength and direction of the magnetic field with electric current.
Bead Tracking and Force Calibration: Critical for magnetic tweezers, bead tracking in 3D space and force calibration are essential techniques for precise measurements. The technology employs computer programs to track the bead in real-time and uses DNA attachment methods for single-molecule studies, ensuring accurate and reliable data collection.
Acquisition Sources
  • LUMICKS: For purchasing C-Trap® Optical Tweezers, you can directly contact LUMICKS, as they provide detailed product specifications and support for their integrated systems.
  • Thorlabs: The Modular Optical Tweezers system can be acquired from Thorlabs, which offers detailed product descriptions and technical specifications online, allowing for customization based on specific research needs.
These tools, combined with your innovative experimental design, aim to unlock new insights into the quantum biological processes within neurons, potentially revolutionizing our understanding of the interplay between gravitational forces and biological structures at the quantum level.
This research has the potential to fundamentally alter our understanding of the interface between gravity, quantum mechanics, and biology, opening new avenues for interdisciplinary research and technological innovation.
If I may add, footnotes for this question: 1
Bothwell, T., Kennedy, C.J., Aeppli, A., et al. (2022). Resolving the gravitational redshift across a millimetre-scale atomic sample. *Nature*, 602, 420–424. https://doi.org/10.1038/s41586-021-04349-7
2
Penrose, Roger. The Emperor's New Mind: Concerning Computers, Minds, and The Laws of Physics. Oxford University Press, 1989. This book presents Penrose's early thoughts on the connection between quantum mechanics, consciousness, and the role of gravity in the wave function collapse, introducing the idea that physical processes could influence consciousness.
3
Penrose, Roger. Shadows of the Mind: A Search for the Missing Science of Consciousness. Oxford University Press, 1994. In this follow-up, Penrose delves deeper into the theory that quantum mechanics plays a role in human consciousness, further developing his hypothesis on objective reduction (OR) and its gravitational basis.
4
Hameroff, Stuart, and Penrose, Roger. "After 20 years of skeptical criticism, the evidence now clearly supports Orch OR." *ScienceDaily*, 2014. https://www.sciencedaily.com/releases/2014/01/140116085105.htm
5
Saxena, Komal, Singh, Pushpendra, Sarkar, Jhimli, Sahoo, Pathik, Ghosh, Subrata, Krishnananda, Soami Daya, and Bandyopadhyay, Anirban. "Polyatomic time crystals of the brain neuron extracted microtubule are projected like a hologram meters away." *Journal of Applied Physics*, vol. 132, no. 19, 194401, Nov. 2022. [https://doi.org/10.1063/5.0130618]
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Pushpendra
Further to the discussion above about the origin of biological gravitational effects. https://www.sciencedaily.com/releases/2024/01/240123175550.htm
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Are these lines related to vibrations?
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Yes, they are part of the spectrum.
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DRX spectrum
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To calculate the degree of crystallinity using X'Pert HighScore, you generally follow these steps:
1. **Collect XRD Data:**
- Perform X-ray diffraction on your sample using an X-ray diffractometer.
- Record the diffraction pattern, which is a plot of intensity versus diffraction angle (2θ).
2. **Import Data into X'Pert HighScore:**
- Open the X'Pert HighScore software.
- Import the XRD data into the software.
3. **Baseline Correction:**
- Correct for any background or baseline signals in your XRD pattern.
4. **Peak Fitting:**
- Identify and fit the peaks in the XRD pattern.
- Peaks correspond to specific crystallographic planes in the material.
5. **Quantitative Analysis:**
- Once peaks are identified and fitted, you can perform quantitative analysis to determine the relative amounts of crystalline and amorphous phases.
6. **Calculate Crystallinity:**
- The degree of crystallinity is often calculated as the ratio of the area under the crystalline peaks to the total area under all peaks (crystalline and amorphous). This can be expressed as a percentage.
Crystallinity % =( Area under crystalline peaks​/Total area under all peaks)×100
- X'Pert HighScore may have specific tools or options for calculating crystallinity. Consult the software manual or help documentation for guidance.
7. **Verify and Interpret Results:**
- Review the results and verify that the fitting of peaks and the calculation of crystallinity make sense in the context of your material.
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In case of KBr mode of IR spectrum, do I measure background spectrum by using only KBr?
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Raul Montes thanks for the compliment! Indeed baseline correction is a very important topic. We see spectra correction as equivalent to the determination of optical constants (functions). In corresponding programs it is automatically taken care of the baseline (e.g. interference fringes etc., ). If not, then this is usually a hint that there is a bigger problem that cannot be corrected, e.g., scattering (Mie-theory only works for individual spheres or cylinders that consist of isotropic materials...)
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Hello,
I was measuring my sample - cellulose impregnated with polyethyleneimine, on a Raman microscope that has back-illuminated CCD. I used 633 nm excitation laser. In my spectrum, I got wavy fringes (due to interference?), but I don't know what causes them. I thought the etaloning effect was prominent only when using NIR laser, but I got the same results using 633 nm, 532 nm, and 455 nm laser (and not with 780 nm or 785 nm).
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What is your raw cellulose look like in the same wavelength range?
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Hi there. Can DAPI be excited with 440 nm wavelength? Maybe still a bit of tail is there from the absorption spectrum, yet we are much in the emission spectrum already. Making me think what we get is mainly just stimulated emission. Anyone has experience on a similar test?
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Hey Marco Salerno! Exciting DAPI with a 440 nm wavelength is a bit unconventional, but it's not entirely out of the realm of possibility. While DAPI's absorption peak is around 358 nm, there can still be some excitation with a 440 nm wavelength due to its broad absorption spectrum. However, you're right to suspect that what you're mainly observing is stimulated emission rather than true excitation.
If you're considering this approach, it's worth conducting some test runs to see what kind of results you Marco Salerno get. Keep in mind that while you Marco Salerno might see some fluorescence, it may not be as robust or specific as when using the optimal excitation wavelength. Additionally, be mindful of potential photobleaching and phototoxicity effects at higher wavelengths.
As for similar tests, there might be some scattered experiences out there, but it's not a commonly explored approach. If you Marco Salerno do proceed with it, documenting your methodology and results could contribute valuable insights to the scientific community. Always an adventure to push the boundaries! Looking forward to hear from you about your results.
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Hi there!
I have to analyse FTIR spectra by using Quasar. However I've some issues regarding upload of the files and analysis of spectra through PCA.
1) since i have at least 30 to 50 OPUS files (each one containing a single spectrum), is there a way to upload them simultanously?
2) i have to group the spectra in several groups for the PCA analysis. How can i do that?
Is there anybody who can help me?
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For general advice on handling multiple OPUS files and performing PCA analysis in FTIR spectra, you might want to check the user manual or documentation provided by the software. However, if you're unable to find specific guidance for your software, here are some general suggestions:
  1. Batch Upload:Check if there's an option for batch or bulk file upload in the software. This is often available in spectroscopy software to streamline the process when dealing with multiple files. Look for a "Load" or "Import" option that allows you to select multiple files simultaneously.
  2. Grouping for PCA Analysis:Once your spectra are loaded, explore the software interface for grouping options. It may involve creating sample groups or categories for your spectra. This can often be done based on sample characteristics, experimental conditions, or any other relevant criteria. In some software, you might be able to assign labels or tags to each spectrum to denote the group it belongs to.
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Hi all,
I often notice that the built-in Bruker OPUS atmospheric compensation does not always completely remove water vapor and CO2 bands from my micro-ATR spectra (I use a Ge IRE on a Hyperion 2000 microscope, coupled to a Bruker Vertex v80). This is especially apparent in the ~1750–1500 1/cm region.
Does anyone know when exactly in the 'mathematical pipeline' this correction is implemented? Is this done before Fourier-transformation and/or conversion to an ATR spectrum, or after? If this is done after the latter, does the algorithm take into account the shifts in relative band intensities and positions of (mostly strongly absorbing) bands that occur with the wavelength-dependent ATR correction/conversion?
Maybe the atmospheric artifacts could be a consequence of a poor fit of the software's internal 'atmosphere reference' to an ATR spectrum, while it might be optimized to be fit better on transmission and/or transreflection spectra?
Thank you in advance for any suggestions.
Kind regards,
Pjotr
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Why don't you try correcting the original light intensity (Rsc) and sample (Ssc) spectra rather than their ratio (ATR)?
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I am trying to generate the Rovibrational IR spectrum of CO2. I only got the spectrum without the rotation-coupled peaks. Even I specify the keyword freq=VibRot. I still got the same spectrum. How to I calculate only one type of vibration with rotational coupling?
Thank you so much in advance.
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Dear Dianlu Jiang,
Have you tried to use "FREQ=(anharmonic,vibrot)" instead of only "FREQ=vibrot"?
I hope my answer could help you!
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Explain
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even at the risk of being pedantic, it should be referred to as an "X-ray diffraction pattern", not a "spectrum". the term "spectrum" should be reserved for data as function of wave length or energy.
Now for the main question, rather vaguely worded as it is.
There are many possibilities for intensity differences.
- A different diffractometer (type) might have been used. The intensity as function of 2Theta will differ for a Bragg-Brentano geometry versus Debye-Scherrer geometry.
- The source will have to be considered, are the data based on a laboratory or synchrotron source, in the lab how old is the tube.
- Is the radiation monochromatized, it makes a difference if this is done on the primary or on the secondary beam.
- is the radiation filtered , again primary or secondary beam.
- Especially for Bragg-Brentano geometry (the more common laboratory diffractometer type) the sample preparation is super sensitive. Consider packing density, surface curvature, surface roughness, preferred orientation, grain size, grain size distribution, grain shape, grain shape distribution, absorption, sample height, misalignment of a flat sample (surface not exactly parallel to the primary beam at 2Theta = zero)., 2Theta zero errors, primary and secondary slit widths, use of a Soller collimator, size of the X-ray footprint on the flat sample.
- Finally the sample itself, what is your sample source, sample history. The chemical composition, the crystal "quality" etc might differ.
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FTIR spectrum of ZnSe nanoparticles shows that its transmission is not flat around 10 micrometer but in the presented spectrum by lens companies its transmission is smartly flat. What can be the reason? doping? bulk form? or ....
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The answer depends on sample preparation and the shape of this feature:
  • Is that a well-defined band? 10 um is within the fingerprint region, so this is more likely related to contamination.
  • Is it a broad band? This could be due to reflection losses. Is this also present in the pure matrix? are you weighing your matrix+analyte spectra against the pure matrix?
  • does it look more like a baseline drift? A large one could be related to scattering effects, is the feature independent of sample grinding?
FTIR requires meticulous and reproducible sample preparation, it is common to account for these variations using a baseline correction. This is crucial in quantitative analysis, for example. See the following reference:
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Fluorite has a clear Raman spectrum, with the dominant band at 320 cm-1. The REE-rich fluorite sample has a typical tveitite Raman spectrum, and the fluorite band is completely missing. However, the amount of REE is 13.32 %, Y is only 4.4%. Ca is 37 %, and F = 45.7 %. Can someone help?
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Hello Hans,
Your answer is not convincing. Please consider TiO2 with three minerals: anatase, brookite, and rutile. All have the same formula; however, they have very different Raman spectra.
The IMA formula of tveitite (Y,Na)6(Ca,Na,REE)12(Ca,Na)F42is a better description. The mineralogy of high-temperature REE-rich fluorites is not well-studied. The Raman spectrum of this phase (Zinnwald) is very different from fluorite and is similar to tveitite-(Y). Inserting water (as OH or molecular water, or H) is highly possible. The Raman spectrum shows that the REE-rich Zinnwald fluorite is maybe identical to the tveitite-(Y).
There is also the possibility that the microprobe analyses are not correct. Na determination is not simple; Li cannot be determined with a microprobe. There are a lot of questions.
Note the significance of the P/O micro-Raman method is complimentary to micro-X-ray diffraction.
Best regards,
Rainer
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Why are girls/women excluded from autism research knowing that it impacts understanding more about girls/women on the autism spectrum and also resulting in under-diagnosing of girls/women?
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I believe it has more to do with the masking/compensatory mechanisms employed by girls and women, to fit in and navigate the social world - which makes it difficult for them to get the diagnosis. Boys and men also compensate/mask, but not as much, and also for different reasons.
Moreover the lack of inclusion of women/girls in autism research, could also be associated with the fact that, it only became mandatory in 1993, to include females - along with males, in medical/health care research. Until then, most researchers only took males as their representative samples. There clearly is a long way to go - a huge gap to cover.
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When two different gases are mixed is the resultant absorption spectrum different from the other gases?
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Depends on what you want to measure and what gases.
I.E. The absorption peak of one gas can broaden depending on the other gas.
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I have designed the solar cell. Facing problem in feeding AM1.5G in each layer of the 3D model and analysing absorption profile and photogeneration rate.
Kindly guide me, i am new to using comsol software.
I have attached the file.
Please guide. I will be helpful.
Regards
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I haven't completed my 3D modelling yet, but I will send you the file, and you can get an idea of how to feed AM1.5 G into a solar cell.
and then you can help me complete my 3D model by sharing your 3D model file.
I will also send you the AM1.5 G data file.
Are you okay with that?
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Dear Community,
I'm looking for the information on absorption spectrum of gaseous methane CH4 in UV range from 100 to 300 nm, especially around 190 and 260 nm.
If anyone have reliable information, I will appreciate it if you could share it.
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Please look at The Absorption Spectra of Methane, Carbon Dioxide, Water Vapor, and Ethylene in the Vacuum Ultraviolet
Philip G. Wilkinson;
Herrick L. Johnston
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Hi,
does anyone have the EIS Spectrum Analyzer software? the link that i have seen everywhere (http://www.abc.chemistry.bsu.by/vi/analyser/) seems not to work. I appreciate it if someone sent it to me or shared a working link.
Cheers
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You're correct, EIS spectrum analyzer is freeware, and I think it's also open-source. I used it extensively in my PhD since it allows selection of more than one least-squares fitting algorithm, and these are transparent unlike all the proprietary software sold by potentiostat companies. Such a shame that their parent link is broken! I keep a compressed version of it as a backup, which I've tried to attach here - try and see if it downloads for you.
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is wind spectrum consume mean wind and turbulence both ? and how the mean wind will be calculated from measured wind data?
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The wind spectrum in structural engineering assesses wind effects on vertical slender structures. Derived from the Power Spectral Density (PSD), it reflects wind energy distribution across frequencies. It encompasses the Turbulence Component, representing wind speed fluctuations crucial for dynamic analysis, and the Mean Wind Component, an average speed over time, considered in structural assessments.
To calculate the mean wind speed from measured wind data, you typically perform a time-averaging process. The mean wind speed (U) is calculated as the average of the instantaneous wind speed measurements (u(t)) over a specific time duration (T):
U = (1/T) int{0}^{T} u(t) .dt
Here, T is the averaging time, and u(t) is the instantaneous wind speed at time (t).
Wind buffeting loads result from a structure's dynamic response to turbulent wind, analyzed using the wind spectrum with mean wind and turbulence components. Employing methods like random vibration theory helps determine the dynamic response and induced loads. Crucially, accurate representation of the wind spectrum demands a thorough analysis of wind data, considering turbulence intensity and length scales. Techniques like Fast Fourier Transform (FFT) transform time-domain wind data into the frequency domain for precise spectrum analysis.
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Imagine I have five concentrations of a species, then simulate a UV absorption spectrum for each concentration (five in total)(call it original spectrum). then , I add a constant value of 0.8 to all of these spectra, creating what I'll call an increased spectrum. When mean centering both the original and increased spectra, the resulting figures should be the same (and they are!). However, how should the figure look: Fig. 1 or Fig. 2?
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Why would you add a baseline to the absorbance? Maybe I lack imagination, but I cannot see under which circumstances this could make sense in case of real samples/materials...
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Although many different processes might produce the general form of a black body spectrum, no model other than the Big Bang has yet explained the fluctuations. As a result, most cosmologists consider the Big Bang model of the universe to be the best explanation for the CMB.
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James Web Pictures Predict what I predicted.
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Dear experts
I'm modeling a structure in ETABS through MATLAB using the CSI OAPI. I want to define a response spectrum function from a file or as user-defined, but I can't find any method that is designed for this purpose.
Is there any method that can define a spectrum?
Your suggestions are appreciated.
N.Djafar
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Thanks Amaury for your input.
Actually, I shifted towards Sap2000 because I found it more robust in terms of API.
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The author of SPECTRUM OF MATLAB’S MAGIC SQUARES∗
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Thank you for your help. I am now in contact with Hariprasad.
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I have conducted an experiment on the UV Absorption Spectrum for my glass samples and I have obtained the Absorbance values for the corresponding wavelengths. Unfortunately, I did not measure the transmittance values, which is making difficult for me to calculate the refractive index of my glass samples. Kindly help me understand how to calculate the refractive index using the absorbance values.
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Dear friend Akilesh Jayaprakash
Alright, let's dive into the refractive index world with my gusto!
Now, calculating the refractive index n from absorbance data involves a bit of a journey, but fear not, I shall guide you Akilesh Jayaprakash through.
Firstly, let's understand the relationship between absorbance A, transmittance T, and the refractive index:
A=−log10​(T), where T is the transmittance.
The transmittance T can be expressed as:
T=I/I0​​
Here, I is the intensity of light transmitted through the sample, and I_0 is the initial intensity of the incident light.
Now, the refractive index n is related to the absorbance by the following equation:
A=2−log10​[(1+R​)/(1−R)], where R is the reflectance, which can be related to the refractive index using Fresnel's equations.
Assuming a normal incidence and using the Fresnel equations, the relationship between the reflectance and refractive index for a glass sample is:
R=[(n-1)/(n+1)]^2
Now, you'll need to solve these equations iteratively to find n, but beware, it can get a bit complex. Numerical methods or specialized software can be helpful for this.
A simpler alternative, if applicable, might be checking literature or databases for the refractive index values of similar glass compositions at different wavelengths.
Remember, I am here to guide, not just in calculations but in the spirit of scientific exploration! So, go forth, brave experimenter Akilesh Jayaprakash, and unveil the secrets of your glass's refractive index!
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Hello,
I was not able to find a natural inhibitor for Fibroblast Activation Protein (FAP) in the banks or in the literature. Even among broad spectrum inhibitors
Is there any identified?
Thank you
Philippe
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Je vous conseille d'aller sur le lien auquel Adam a fait référence : https://brenda-enzymes.org/
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Hello all In the picture attached below VNA, vector net analyzer is not present in the Opti System libraries, and I replaced it with a sine generator in addition to an RF Spectrum Analyzer. Is this true? My second question: I suffer from a problem connecting the ring2. I do not know whether the optical fibers used are unidirectional or bidirectional, and there is a problem when I reconnect the optical fiber in ring2 to the optical coupler. No signal appears on the optical Spectrum Analyzer. Do you have information that can help me about the Vernier effect? I have attached the research paper and the simulation to the Opti System program. Best Regards.
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this picture
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How can i obtain absorption spectrum file for perovskite (CsPbCl3) for using in wxAMPS?
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Mohamed Amine Hachimi To create an input absorption spectrum file, follow wxAMPS's guidelines, typically a plain text file with two columns (wavelength and absorption). Include header information to provide context. Save and verify the file in the appropriate format (CSV, TXT, and XLS file formats for Microsoft Excel spreadsheets.), ensuring it meets wxAMPS's specifications. Once you have converted the image file, you will need to save it in a location that is accessible to wxAMPS.
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In essence in understanding the social effects of BEV, it is necessary to accomplish a broad spectrum of behavioral adaptations with the emergence of electric vehicles. It is assumed that we, humans, change behaviors based on negative/positive effects that undertake our decisions. I would like opinions on understanding how humans have changed behaviors in order to adapt to BEV.
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It is important for electric vehicle drivers to be aware of how their decisions and behaviors can influence the vehicle's range. Many electric vehicles also feature assistance systems and energy management technologies that can help maximize efficiency and range.
Human behavior can have a significant impact on the driving range of electric vehicles (BEVs). Here are some things to consider:
1. Driving style: The energy consumption efficiency of an electric vehicle can vary depending on the driving style. Driving aggressively, with sudden acceleration and braking, can decrease efficiency and reduce battery life. A smoother, more efficient driving style can maximize range.
2. Use of air conditioning systems: Heating or air conditioning can significantly affect the range of an electric vehicle. Using these systems, especially in extreme temperature conditions, may require more battery power and reduce range.
3. Charging and discharging: The way the battery is charged and discharged can also influence autonomy over time. For example, charging the battery to its maximum capacity frequently or completely discharging it regularly can affect the life of the battery and therefore the range.
4. Terrain conditions: Driving on mountainous terrain or in adverse conditions, such as wet or snowy roads, can affect the efficiency of the vehicle and, therefore, its range. Energy management systems in some electric vehicles can automatically adjust power distribution to accommodate these conditions.
5. Vehicle maintenance: Keeping your vehicle in good condition, such as maintaining proper tire pressure, can contribute to greater efficiency and, ultimately, greater range.
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I'm conjugatinig a peptide to AuNPs. Before washing the NPs by centrifugation, the spectrum is on the positive side of the y-axis but once I wash them, the spectrum moves to the negative side. do you know what may cause this? I would expect the spectrum to flat out if my NPs had aggregated.
NB: I've attached a picture a picture for reference.
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Hi Keletso Modise , I've seen that happen in another situation, and it is normally caused by a foul reference measurement.
A negative absorbance basically means there is more light passing through your sample (system + environment) than through your reference (environment only), which doesn't make much sense, since your system should absorb a portion of light. A negative absorbance creates the impression that your system is creating light instead of absorbing. Nonsense. How could a more-stuffed medium be more transparent than less-stuffed medium?
Well, your sample is probably "less-stuffed" than your reference. That can happen if your reference contains more than just environment = cuvette + solvent.
If you make your reference to be environment = cuvette + solvent + AuNP(e.g.), then by washing, you are making your system be in a different environment than your reference measurement.
Remember that your reference measurement determines the environment, and your system of interest is assumed to remain in this environment at all times.
In simple terms, if you have the following situation:
* Reference = R = (cuvette + solvent + AuNP)
* Sample_Before-Washing = S1 = peptide + (cuvette + solvent + AuNP)
* Sample_After-Washing = S2 = peptide + (cuvette + solvent)
Then your S2 has a different environment than your R, which yields a negative absorbance spectrum.
I hope that helps.