Skin Cancer - Science topic

I am trying to culture NTERT immortalized human keratinocyte cells.

I found several suggested medias:

Keratinocyte serum-free medium (K-sfm) (GIBCO/BRL) plus 30 μg of bovine pituitary extract per ml, 0.1 ng of EGF per ml and additional CaCl2 to raise the [Ca2+] to 0.4 mM 

or

3:1 DMEM-Ham's F12 supplemented with 10% FBS, 10 ng/ml mouse epidermal growth factor (Serotec), 1 ng/ml cholera toxin (Sigma), 400 ng/ml hydrocortisone (Sigma), 5 mg/ml insulin (Sigma), 5 mg/ml transferrin (Sigma), 13 ng/ml liothyronine (Sigma), and 2 mM l-glutamine (Invitrogen)

or 

KGM-Gold complete medium from Lonza

Anyone who has them in culture and can suggest the best medium?

Thanks!

Hi everyone, I'm working on skin cancer classification and I've extracted the feature from three pre-trained CNN models and concatenated all the features. Finally a dense layer with softmax function was used for classification.

But I encountered constant validation accuracy with lower training accuracy.

How can I solve this problem please. Despite this, I have tried many optimizer functions with different learning rate, regularizer, early_stopping, dropout, and also I used image augmentation

What's the reason for the chosen choice of inferential statistics?

I am currently performing co-culture experiments with murine CD8 T cells isolated from mouse spleens and with skin cancer cells (PDV) in a 24-well plate. I first isolate the CD8 T cells from spleen which are around 70% alive at time of isolation, mix them together with CD3/CD28 T Cell activation beads to activate the CD8 T cells, and plate them 2x10^5 T cells on top of 2x10^5 skin cancer cells that were plated the night before. The cancer cells are treated with mitomycin C prior to plating the T cells in order to halt cancer cell proliferation. Then I let them incubate in a tissue culture incubator with standard parameters (37 degrees Celsius and 5% CO2) for 5 days. In addition to the wells where I co-culture the T cells with the plate cancer cells, I also plate some of the T cells with the activation beads in an empty well to act as a control. I then assess the viability of the T cells by staining with a live dead stain and running flow cytometry. However the results of the flow cytometry show show that most of my T cells are dead (<40% viability). The most problematic issue is that the T cell-only wells come up only 1% viability which render the rest of the experiment null since I would not have a T cell control to compare the T cell viability from the T cell/cancer cell co-culture wells.

The interesting observation is that the T cells co-cultured with cancer cells have greater viability compared to the control T cells cultured by themselves which makes me think that the cancer cells are stimulating the T cells and improving T cell viability that way. I was wondering if there are any adjustments I could make to improve the viability of my T cells especially for the T cells in the T cell only control wells?

Some adjustments I have made already:

1. Increase the speed of the T cell isolation from the spleen to improve baseline viability prior to seeding on the cancer cells

2. Utilize wide bore tips when pipetting T cells to decrease the shear force on the T cells

Thank you for any suggestions and apologies for the length of this question.

Hello everyone, I want to enhanced the medical image of skin cancer as well as feed the enhancement image to CNN for classification.

I wonder if generative adversarial network (GAN) is the best method? And how can I use GAN for the purpose of enhancement instead of generate a fake image.

there any study connected between dermal filler and skin cancer .

I am updating my earlier review on the role of Fluoride doped Hydroxyapatite in Cancer and my current focus is on Psammoma Bodies which have been found, and identifed as high risk, in a very wide range of Cancers. These include Cancers of the Bone, Spine, Brain, Choroid Plexus, Dura Mater, Gliofibroma, Medulloblastoma, Meningioma, Cervix and Endometrium, Ovary, Kidney, Lung, Mesothelioma, Pancreas, Skin, Hemangioendothelioma, Olfactory Neuroblastoma, Duodenal Somatostatinoma, Stomach and Thyroid. Early studies did not have the benefit of advanced analytical techniques, or did not even consider the Fluoride content or composition of the mineralization. Can anyone help by supplying analytical data based on Raman spectroscopy, neutron activation, x-ray or wet analysis?

Can anyone please share Skin cancer(melanoma) dataset please.

I am working on a deep learning-based analysis to detect Melanoma Cancer. I want to use all types of algorithms to get the best possible solution.

Basal cell carcinoma (BCC) is the most common form of skin cancer. An estimated 4.3 million cases of BCC are diagnosed in the U.S. each year. Squamous cell carcinoma (SCC) is the second most common form of skin cancer.

actually I searched the related web sites, but the quantity of samples are maximum 20 or 30 pictures, but I saw in articles that there are datasets from theses 2 sources with enough number of samples (more than 100).!!!

I am doing a research on Skin Cancer Detection. I have read a lot of redundant research papers about the problems which related to the same topic. Therefore, I wish to know the recent research directions of skin cancer detection.

Hi, is there any dataset about occupational skin diseases (especially skin cancers) that contains skin lesions images (simple or dermoscopic or histologic) ,integrated with clinical data and occupational history (e.g. to see if they have had occupations with carcinogenic exposures particularly UV)

I want to use it to make a research on the contributions of these exposures in the development of skin cancers and the possibility of early detection of the malignancy of these lesions by data mining and image processing considering occupational exposure as an important parameter

any suggestion is appreciated

I am working on skin cancer on female swiss albino mice. I am inducing skin cancer by topical treatment of DMBA and the other group DMBA+ cationic peptide treatment (Cationic peptide is given sc) I will be studying mainly the expression of apoptosis proteins bcl2,bax and cytochrome c. Want to know how can I preserve that for about 1 month this skin cancer tissues by liquid nitrogen and keeping in -20 degree deep freeze. whether it will be fine to preserve that way. plz help 

How can we assess the presence and amount of T-cells in the freshly removed skin tissue after surgery or punch biopsy? Also, if we freeze the skin tissue at -20C for a long time, after thawing to warm PBS or water, how can we make sure we have T-cells especially CD4 and CD8 T cells in the extracted frozen skin tissue? Does skin have to go in a specific solution or it has to be assessed for the specific markers ?

A prospective study has been published by Wen-Qing Li (JAMA Intern Med. doi:10.1001/jamainternmed.2014.594, online April 7, 2014) raising an intriguing question, stating that Sildenafil use for ED showed a statistically significant elevated risk of melanoma. We´re really concerned about this. Any insight regarding this important subject?

I am doing research on melanoma detection for dark skin people. so it's very difficult to identify the dark skin images, please tell me the idea to get the dark skins.

This question is defined in greater detail in my blogpost 

I am working on ISIC skin cancer image segmentation in my project i got high dice coefficient score, high recall, precision but when i evaluate the ROC and PR curve i do not get good result can any one explain this.

Thanks very much

Skin bleaching ( whitening ) creams have a negative impact on the users, including the prospect of causing cancer, skin diseases ( dermatitis ) and even secondary bacterial infectiins. Did you agree? If yes what chemical contents in the creams cause the above mentioned impacts.

i can't find ground truth image for HAM10000 dataset.

The question my project aims to answer includes ...Does providing skin cancer risk assessment questionnaires as an intake process before a patient examination, increase skin-related interventions by primary care providers? Patient questionnaires will be available for 30 clinical days with an estimated sample of a minimum of 50 completed questionnaires. The questions include the generic including; age, gender , and ethnicity as well as about ten yes/no questions with corresponding points to determine if a patient is 1) high-risk or 2)low-risk. The questions range from eye color, hair color, to history of sunburn. The main outcome I want to identify is if by conveying their risk status to their PCP, I want to determine if their risk status lead to corresponding interventions which will also be a check box with answers such as referral to dermatologies or provided full body skin assessment. There will be no comparison of provider interventions without the questionnaire nor will there be any pre or post tests. Any help would be beneficial.

Papers related to Melanoma Skin cancer using Image processing and the latest methodology being Incorporated to identify the Cancerous cells?

I have faced a strange results in my previous experiment.

I had Melanoma cells in culture and after 4 days, I wondered when I saw that the melanoma cells were morphologically changed to a neuron like cells or more likely to melanoblast cells.

The most exciting part of this morphological changes, that prevent me to ignore, was that their growth have been stopped or significantly decreased without applying any specific/new chemicals.

So, I would like to know about any non-specific reason behind this phenomena.

Considering that, this morphological and metabolic changes were happened for a malignant/invasive type of skin cancer cells, the answer may results in a way to step forward in cancer treatment.

Best Regards

Behnaz Ghaemi

I want to find out if skin tatoo has any effect in skin cancer

Hello, 

I am planning to start a project on UVB induced skin cancer. For that I read articles and found the different methods used by the researchers for UVB, but I am unable to identify the design of the apparatus. It will be highly appreciable, if someone can guide me to the design of the chamber.

- low dose ASA is in discussion to increase skin cancer in male adults.

- ASA showed significant decreased thymus-size in animal studies.

- Should we create studies to clear this question ?

Is their any data in neonates or children after longterm low dose ASA in pregnancy?

In Germany we observe an uncritical use of low dose ASA in reproductive medicine and every kind of previous pregnancy complication.

Melanomas and sun related skin damages occur exponentially in the last decades. Primary care providers are the perfect professional group to screen the skin of patients.

Recently, i've studied skin cancer. I used A431 and SK-Mel-28 both skin cancer cell line.

When i transfected "A" gene into two cell lines, but cell proliferation and migration were totally different.

I searched on the web what makes different results between two types of skin cancer.

Many papers showed effect of gene over-expression and knockdown in both skin cancer is almost the same. I did not found different effect on non-melanoma and melanoma.

Could you give me introduce some papers or explain why this results produced?

Anti-pollution cosmetics is gaining lot of attention worldwide. The pollution can be from sunlight, air and electromagnetic or optical devices. Pollution can affect health particularly the skin, hairs and eye.

Pollution creates excess free radicals which can damages the skin microstructure and components such as lipids and initiate inflammation, hyperpigmentation, aging process and skin cancers.

Many cosmetic products claiming anti-pollution properties with some data. However, we do not know what are legit techniques and gold standards. As such regulatory agencies have so far quite on this issue and are not providing any guidance to consumers and cosmetic companies. This is dangerous as many un-safe cosmetics can be sold in the market. Therefore, we need to work together to find gold standards and help consumers. Hope this initiative will trigger more interest and participation worldwide. Thank you.

Where can i find a good dataset of at least 200 images of melanoma

Hi,

I'm looking for average skin tone data by country. I found one on target map but I couldn't find its resource. Does anyone have an idea?

Thanks

Dror

          I am researching the potential benefits and decreased health risks of providing man-made, cultivated, and/or natural shade for public playgrounds. Benefits may include increased participation in play and socialization, and decreased risks may include decreased exposure to excess sun and heat at outdoor public playgrounds, including: dehydration; heat injuries, including heat cramps, heat exhaustion, and heat stroke; thermal burns from overheated surfaces and equipment, including plastics and ground cover; and injuries from exposure to ultraviolet rays of the sun, including sunburn and skin cancers such as melanoma. 

          This question is related my Capstone for my Master in Occupational Therapy degree. I appreciate any feedback regarding search methods, existing research, and guidelines for pursuing the question further.

I am developing a medical device (orthopedic prosthetic), during application I want to use UV curable adhesive for fixing and cavity filling. for that I am planing to use High power UV led light having peak wavelength of 365 nm and intensity is ~100mw/cm2. I coulden't find any confirmed data on safety study over skin. exposure is one time and it would last for 3-4 minutes.

Should I go ahead with above UV LED light ?

Would you please help me to find safety study for this ?

Hello, I plan to start inducing skin cancer in Wistar rats, but since we do not have an absorbent hood in our lab, so I would like to know, please, if i can use a chemical mask that i bougth online from this site;

(N° 3 cartridge are Normally impermeable to organic chemical vapors such as acetone and several other organic chemicals)? and can I use safely this mask and work a dorsal applications of DMBA in outdoors so in the open air?

If this is not feasible, is the application of 1% DMBA / 1% croton oil on rats capable to causing skin cancer in wistar rats because I did not found a scientific article 'paper' that talks about?

thank you very mutch 

Thanks in advance for your replies.

It is quite easy to find databases with images of skin cancerous, non-typical moles. It is however, quite difficult to find images with normal moles.

Obviously you need both types to train your classifier.

Any suggestions are highly welcome.

This question is specifically asked within the Project called "Treatment of Melanoma Brain Metastases."

Because neurological tissues as well as many forms of skin malignancies will tend to express at least some significant CB1 and CB2 receptors, perhaps using moderate dosing of a safe agonist like Delta 9-THC may be useful?  And consider including evaluation of equivalent to 100-300 mg or oral CBD for humans as well as this is becoming very common among patients.  

I work with both brain cancer patients (mostly Glioblastoma) and many breast cancer patients and they had already chosen to integrate cannabis extracts into their therapies. Because CBD and THC cross the BBB, and does not appear toxic to healthy normal cells, it may be reasonable to consider exploring this with research. I do have one interesting patient who reported using these compounds to treat her ER-PR-HER2+ brain metastases with tremendous success in only 3 months. The Herceptin and Perjeta she was on are too large to cross the BBB, so the situation is dire for her otherwise. Best wishes and thank you for your project!

I am trying to download the ISIC2017 training and testing data but I didn't find any link. I tried the ISIC2016 I found the Training data but there is no TEST data.

Here is link: https://challenge.kitware.com/#phase/5840f53ccad3a51cc66c8dab

Regards,

I would like to analyse proapoptotic activity of a compound to be used on Actinic keratosis. Is there any cell line that might represent early phases of photocarcinogenesis?

Dear Scientific Community :)

A colleague recently tested his melanoma (ME1402) cells for mycoplasma and obtained some strange images of rod-like structures - quite different from the 'dot-like' beads on a string look of mycoplasma. Please a look at the attached image and provide some insight.

TIA

I am extracting RNA from skin cancer samples. The pipette was contaminated with crystal violet (the undergrad might have sucked in crystal violet while working with it). 

Now I am facing a problem which is a less intense 28S band on the agarose gel for my samples. Is that because of crystal violet? and do you think this crystal violet intercalation with RNA would affect the RT step and PCR?

Thanks

I need melanoma skin cancer images dataset, kindly help me out, suggestions will be appreciated. 

Regards,

Hi,

I was performing MTT assay in my lab hood. Accidentally, I did not turn off germicidal lamp (UV-C 254 nm) in the hood and I worked for about 25 minutes. I was wearing latex gloves and my hands and arms were completely covered in my labcoat and also my winter coat. My face however was uncovered and I was wearing my glasses. There was hood glass between the lamp and myself and the distance between my face and lamp was as usual as in normal airflow hoods. 

This happened 4 days from writing this post. After overexposure, I did not see any symptoms like burns in my cornea or erythema so far. I am really scared as to what fate awaits me after 6-12 months as they say this can lead to skin cancer. 

I would be really grateful if anyone could suggest on what I should do and what fate awaits me in the next one or two years.

Regards,

Pratik

Is there any benefit in irradiating a skin tumor that has necrosed after cetuximab and shows a solution of continuity with the subcutaneous cellular tissue, although it is still present (partial response) and it is associated with local lymphadenopathies? Will the wound ever heal?

can I have the reference as well?

Or does cobimetinib not work any more if a patient does not answer to vemurafenib/shows progresssive disease? Would you switch to a PD1 inhibitor?

The European union is hearing evidence on whether the lower the amount of topical vitamin A that may be used in a cosmetic.   Vitamin A is almost completely locked in the skin because there are no transport mechanisms to take it form tissues into the blood stream so topical vitamin A can never be absorbed into the  blood and poses no risks, even at high topical doses.  We need to show that higher levels of topical vitamin A reduce skin cancers, and make skin healthy.  High doses of oral vitamin A may well reduce alzheimers.  Vitamin A in conjunction with vitamin D keeps bones healthy.  I have used 50,000 i.u. per day for 22 years and my bones are better than average for 26 year old people on scanning.  We know already that certain leukemias respond to vitamin A.    I am collecting evidence and would appreciate as much feedback as possible.

Hi everyone,

does anybody work with the melanoma cell line RPM-MC and knows whether they contain mutations in either BRAF (e.g. V600E) or NRAS (e.g. Q61R/K)?

Thanks a lot in advance!

   

Dear All,

I'm using DMBA/TPA to induce the skin cancer in Balb/C mice, I would like to know how does my compound of interest in inhibiting the growth of tumors. What are the mechanisms that are involved in the inhibition of the cancer in the skin of Mice.

Please help me to get a clear concept of this phenomenon.

Thank You

The etiology of skin cancer is different in Black skin compared to White skin, and excess sun exposure is not the only risk factor. Black Africans do experience sunburn and cataracts, so a public health communication tool relevant to dark skin may be important.

Hello to everybody,

 I want to isolate all the leukocyte populations (CD45) from B16F10 tumors (injected in mice subcutaneously). Can someone recommend a good dissociation media (like PBS or complete RPMI)? In particular, which enzymes would you use? I know that some of them can degrade surface antigens.

Thank you very much,

Michele

Is U.S. public health policy on sun protection based entirely on epidemiological evidence? Has there ever been an RCT on sun protection and skin cancer?

As far as I am concerned, the standard coverage 50x for the analysis of highly heterogeneous tumor samples such as melanoma is insufficient and scientific literature recommends base coverage around 500x or even 1000x. However, with that coverage the cost of analysis increase enormously.

Hi Guys! i will appreciate if you can answer the following questions:

1- which cell line(s) is used to study keratinocytes and their potential UV-induced malignancy formation?

2- is there a mouse model for that? ✓

3- is there mouse line in which UV radiation causes visibil skin damage? 

Many thanks in advance.

Cheers!

Peter

Hi. Recently I used GeneChip® miRNA 3.0 Array - Part #902018 (Affymetrix) to make a microRNA profile of skin cancer cells. First of all, I transfected my cells with hs-miRNA-34-5p, and the transfection increased 327- fold change when compared to non-transfected cells. How can I find information about level of microRNA transfection? I want to know if this concentration of microRNA are toxic to cells, if inhibits another endogenous microRNA processing, or if this fold change is good.

All miRNAs found in high or low levels will be validated by qRT-PCR. However, I wonder if I could use some miRNA microarray database to compare my results with other cells types. Like in silico screening of frequency alteration of microRNAs expression in skin cancer lines. Could someone suggest a database to do this search? I really need help with Bioinformatics.

Background: Preventive and health promotion projects aim to induce healthy habits in children through education about sun exposure in order to protect against and reduce the incidence of skin cancer. Education about the prevention of skin cancer is most effective when it is taught as part of a health education curriculum.

Our program was named as "Sol, Amigo da Infância - Sun, Friend of Childhood". This program was established on January 2013 by SBD-RESP board. The program partner schools received materials to facilitate the classroom instruction, and the program also encouraged the implementation of structural measures (such

as planting trees) and individual measures (such as encouraging the use of hats, appropriated clothing, sunglasses and sunscreen).

The program is composed by flexible elements that can be used independently or as supplements to the curriculum of existing health promotion in school.

Aim: The program "Sun, Friend of Childhood" is a school health education and disease prevention project directed to instruct children and educators through activities in school that aim to encourage the adoption and maintenance of behaviours that ensure proper sun protection and the consequent prevention of skin cancer.

A total of 194,000 children and 800 teaching coordinators of public and private schools received lessons and activities about our Educational program on photoprotection between 2013 and 2014. HQ´s comics, DVD movie and teachers involvement have been showed effective to disseminate these informations.

Hi everyone,

I am doing spheroids experiment in 96 ultra low attachment well plates. Unfortunately, I faced several problems when I use 96 low attachment plates, so I decided to go with the gel experiment. So how I can do gel experiment for 3D spheroid in skin cancer cell lines.

Thank you so much.

I am trying to compare x ray with UV radiation and was wondering if someone knows how much UV C is necessary to have the same cell killing as 1 Gy x ray? I searched the net but it wasn't really fruitful so far...

Recent experiments revealed that both IR stress and DMBA treatment increase the stem cell factor (SCF) expression level at the secondary hair germ and dermal papillae. However, IR stress causes hair graying due to the damaged melanocytic stem cells (McSCs), while DMBA treatment causes the hyperpigmentation of coat hair in the dorsal skin. This result provokes two important questions;

1) SCF/c-KIT signal is the only signal pathway to maintain the differentiation of immature melanocytes also referred to as melanoblasts or McSCs?

2) What is responsible for the paradoxical phenotype between enhanced SCF expression level and coat hair color?

there are a lots of micro RNAs effect in skin cancer but I need to know the most one that change in skin cancer.

See above

Hi, Is it possible to induce keloid in skin of lab animal? If yes, Could you please introduce me any papers?

can someone tell me best Methods to transfect MCC cell lines. I tried Lipofacamine but it did not work .

Hi! I wanted to know if melan-a cell lines are immortalized with hTERT and if they carry any other mutation (p53, CDK4...)? Thank you very much.

I'm looking for murine malignant melanoma cell lines which are BRAF wt and BRAF mut. We already have B16F10 (BRAF wt) in the lab and are in the process of procuring SM1 (BRAF mut.), but I would like to have at least one more BRAF wt and one BRAF mut. if possible. Does anyone know of any good cell lines? I've found it hard to find any commonly used murine cell lines in the literature with the exception of B16.

Thanks for the help!

I am working on hpv5 e6 oncoprotein expression in skin cancer biopsy  by RT PCR (not real time) and I get only smear bands, how could I ensure that my results are negative or do I have pcr problem

I would like to read some pubblications about melanomapt1a prognosis and risk of recurrance

Can anyone tell me one good buffer which works best to isolate type I collagen from murine skin samples to detect it through western blots?

I have homogenized skin tissue and lysed in NP40 (normal lysis buffer) and also boiled directly the skin homegenate in BSB, in both the cases i could not see Type I collagen bands. Please suggest.

In such cases, one questions himself about his ability of early recognition of melanoma, which is of great importance in the right on time removing of the nevus (or melanoma).

In your opinion, is it more a question of ignorance (you do not see, because you do not know) or could it be attributable to the nature of the tumor that can appear so suddenly and abruptly?

An elderly woman who complained of pain and progressive erosive lesion measuring 5cms * 4cms eroding the ear lobe. Duration 2-3 years. Complained of pain down the neck. urface was rough with erosion at places, margins elevated uniform with a distinct line of black color, soft to touch, tenderness was present.

No lymph nodes were palpable, hearing was good, no vertigo, no diabetes, mild hypertension was told.

History of moderate sun exposure noted.

Probable diagnosis of basal cell carcinoma was made.

She is poor, and single.

Can I get some inputs regarding the case? Open for any questions

les

I am using 5 FU liposomes for transdermal delivery but I am confused a little bit regarding how much formulation must be loaded into donor compartment of the Franz Diffusion Cells for 5 FU liposomes which must be cytotoxic to cancerous cells. I browsed the internet for weeks but didn't find a satisfactory answer since all topical available formulations of 5 FU state to "apply twice a day". But no answer to the question of exactly how much 5 FU must be applied.

Can anyone guide and help me to promote the growth of human melanoma cell line (SK-MEL-2) and murine melanoma cell line (B16). We bought both of them directly from ATCC 2 months ago. At begining they were grow very well (DMEM media + 10% FBS+ 1% antibiotic and antimycotic) and we prepared about 10 stocks from each. Now we want to thaw and regrow again, but we can not. The cells are not growing at all. TQ 

Estimation cyclooxygenases directly or by its products?

In order to cultivate keratinocytes with an air-liqiuid interface you have to de-epidermize human skin flaps in one step of the procedures. This is described by Prunieras M, Regneir M, and Woodley D.

To de-epidermize skin flaps you have to maintain the flaps in PBS at 37º for several days.

The PBS I used consists of NaCl, KCl, NaH2PO4, KH2PO4, Mg Ca Free. X1 concentration.

My wife died less than three months ago due to complications in melanoma. Truly a sad day for my family. I wish there was some reliable way to detect it early since it progresses rather fast. And could anybody share some insights on what triggers it? All I could find are hormonal changes, etc... but could anybody more specific?

Thanks guys.

There is no current evidence to support the use of aqueous cream versus any other moisturising brand or indeed using nothing whilst undergoing radiotherapy treatment. Has anyone looked into doing any form of trials to determine which works best?

I want to do FISH for two regions of chromosomes. One region can be variable in length, and I want to measure its length and length variation. Other region should be least prone to chromosomal rearrangements, so I could analyse fluorescent signal intensities ratio to estimate length of the region of interest. Which region is the least prone to chromosomal rearrangements in melanoma? Is using D2Z okay? What other region would you suggest, and which probe would you use?

I am interested in polarimetry imaging of skin. I would like to use fuzzy logic on polarized images to extract information about skin cancer and its diagnosis.