Silver Nanoparticles - Science topic

Hi, can you help me with this? What should I consider first before I start formulating a method to form a gel extract to cure oral ulcers?

- I want to test different concentrations of the marine-based extract with the silver nanoparticles, and Carbopol 940 as gelling agents (in vivo)

if using probe sonication method for green synthesis of silver nanoparticle what should be the optimized frequency and timing of probe sonication to get silver nanoparticle peak in range of 400-500nm and also what to do if the plant extract is acidic in nature.

what should be added dropwise plant extract to silver nitrate or silver nitrate to plant extract and also suggest me the optimized concentration of plant extract and silver nitrate ?

I am trying to synthesize silver nanoparticles from a plant extract. But lower concentration of AgNO3 solution is not enough to obtain my desire UV spectrum for the characterization. So I want to increase the concentration of AgNO3 solution (5 mM). Generally 1 mM of AGNO3 solution has been recommended for in vivo test. May I try 5 mM of AGNO3 solution for my in vivo experiment?

I'm working on developing a fungal suspension for pest control. I've come across many research papers utilizing silver nanoparticles (AgNPs). Could someone explain why AgNPs are so commonly used in pest management? Considering their high cost, are there any effective and affordable alternatives?

The photo is for a thin film obtained from Ag-MC-nanocomposite. The nanocomposite was prepared using silver nitrate as a precursor, methyl cellulose as a capping agent, and plant extract as a reducing agent. This is the second time I see this amazing shape of my dried film. The shape looks like tree branches formed in a regular manner. .Does this indicate, for example, the crystalline properties or purity of AgNPs? Please, I want an explanation for this shape, especially this is the second time I see this shape after drying the film but using a different plant.

I am working on green synthesis of silver nanoparticles. I am getting particle size beween 100 to 150 nm. I have increased the stirring speed up to 2000 rpm. Still I am unable to get particle size below 100 nm.

Please find my recently published paper at the following link: https://rdcu.be/d41UP

Silver Nanoparticles docking with alpha amylase and alpha glusidase .

I want to study the Genotoxicity of Silver Nanoparticles using the Ames Test. But, I am unable to procure the Tester strains of Salmonella Typhimurium. Can someone help me out in procuring the strain in India?

I am trying to synthesize silver nanoparticles from aloe vera gel extract prepared by cold extraction method of equal ratio of gel and water and i have prepared silver salt stock solution of 50ml. can anyone expert tell me which concentration of silver salt from stock and gel extract and distill water should i use for making silver nanoparticles. it will be very helpful.

What is the role of silver nanoparticles and quantum materials in predictive analytics for healthcare and solar cells?

Hi all,

Please, who knows why EDX of biosynthesised silver nanoparticles is showing some weight percent for zirconium and niobium after biosynthesis? I did not use both in the process. Any idea, please?

Hello, I am studying on the green synthesis of silver nanoparticles using a plant extract and a bacterial component. I am currently in the optimization stage and the UV spectra appeared short and broad. Additionally, the spectra doesn't progress smoothly. Besides, there are two peaks; the peak at 420 nm which is characteristic of silver nanoparticles and a peak around 300 nm also appears. I need help about how to eliminate these issues and optimize the best concentrations. Thanks in advance,

Best regards

What is the method of preparing silver nanoparticles?

dispersion of silver nanoparticle powder

Good day! We are undergraduate students conducting research on the biosynthesis of silver nanoparticles using cauliflower leaf extract. May we ask on why the absorbance peaks of our synthesized nanoparticles are not converging with literature values. What could be the potential solution to this step? Thank you for your time and we hope to hear a prompt response.

I am working on the synthesis of silver nanoparticles, I am getting a peak at 408 nm but it is not a sharp peak and the absorbance is very low at 0.6, after running the reaction for 24 hours. How do I solve this issue? Can I use that for further characterization?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

After finish the synthesis of nanoparticles.and nanoplates of silver how to take out those as solid/powder form from the solution. Please give a solution of u already done that and succeeded by that

swelling release mechanism of polymer undergo diffusion for silver release

I'm trying to produce silver nanoparticles using plant extract, but I didn't observe the expected peak in the UV-Vis spectrum between 380-420 nm, where silver nanoparticles typically appear. After centrifugation, I obtained pellets suspected to contain AgNPs. Based on the provided UV-Vis spectrum, can it be inferred that AgNPs have indeed formed? Where might the AgNP peak be located, and is it possible that it's shifted outside the usual range?

Additionally, both my extract and the silver nanoparticles have a pH of 4-5. I'm curious about how I can adjust the conditions to make them more alkaline and optimize my "green" synthesis.

I would greatly appreciate any insights or advice on these questions. Thank you in advance for your help.

Is the high concentration of AgNO3 solution like 0.05 used for synthesizing green silver nanoparticles effect in synthesis of green Ag NPs using plant extract as a RA?

The as the solution were mixed together, there was no color change, and then the UV-vis was read every 10min but barely any absorbance was observed. The solutions were 2,1mM of NaBH4 and 0,07g AgNO3 in 400ml of H2O. The Mixture of both was 30ml of sodium borohydride and 1ml of silver nitrate, and was placed in a ice bath. Then we expected the color to change but it never did

1. Which form of plant extracts shows the greatest potential for green synthesis of silver nanoparticle:

- direct homogenization with deionized water followed by filtration or centrifugation, or

- initial maceration with ethanol to form a semi-solid macerate later dissolved to a certain concentration with deionized water and filtered? Or can both methods yield effective results?

2. Should I dissolve the AgNO₃ and plant extract in deionized water, or can distilled water or even ethanol be used in the synthesis procedure?

I would greatly appreciate any insights or advice on these questions. Thank you in advance for your help.

I have used 50 ml 1 mM silver nitrate solution and 5 ml extract and 30 microliters of base

I have synthesized AgNP prepared from a plant leaf extract and AgNO₃ solution. It underwent optimization by applying different parameters (reaction time, pH, concentration, etc). The solid NPs were collected after centrifugation and drying. Now, I want to collect and compare the absorbance spectra of the different sample trials, along with the plant extract, and AgNO₃. Kindly help how should the preparation of the solid AgNPs for the UV-Vis. (what should be the ratio between the amount of solid AgNP and solvent to be dissolved in?)

Initially, I conducted maceration with a 70% ethanol solvent for 3x24 hours to obtain the thick extract.

For the AgNP synthesis, the thick plant extract needs to be dissolved using deionized water as a solvent. However, upon dissolution, a significant amount of precipitate forms. I sonicated it for 20 minutes to aid dissolution, yet there was still precipitate present. Subsequently, I filtered it, resulting in a clear extract solution.

However, the resulting clear solution is unstable, even after storing it for only a day in the refrigerator, as precipitate forms again despite initially being a clear, filtered solution.

Are there any suggestions regarding storage or procedures for preparing the extract? Is it okay to filter the extract? Are there any suggestions regarding which filter paper to use?

*I do not use water as a solvent during maceration to ensure obtaining a thick extract so it will not be hard to determine the final extract concentration.

I would greatly appreciate any insights or advice on these questions. Thank you in advance for your help.

The silver nanoparticles were synthesized using plant extract as the reducing and capping agent.

Is it more effective to optimize the ratio of plant extract to AgNO₃, or should I adjust the final concentration of AgNO₃ in a fixed concentration of plant extract, considering the optimization of both AgNO₃ and plant extract concentrations?

Many references suggest that the formation of AgNPs is characterized by a single peak at lambda 380-420 nm. Additionally, are there any guidelines regarding the timing of characterization? I've come across a paper stating that UV-Vis characterization is typically conducted 24 hours after AgNP synthesis to ensure the presence of a peak around 400 nm.

I would greatly appreciate any insights or advice on these questions. Thank you in advance for your help.

Best regards,

In the green synthesis approaches of silver nanoparticles, do plant extracts solely influence secondary metabolite compounds that act as reducing agents and antioxidants? Or do the antibacterial, antifungal, and other biological activities of the plant extract also impact the formation of silver nanoparticles?

I am planning to synthesize AgNP using plant extract as a bioreductor.

I have come across several papers recommending a neutral to alkaline pH range (pH 7-8) to achieve silver nanoparticles with characteristics such as small size (<100 nm), uniformity, and stability. In green synthesis approaches, is it acceptable to use chemical reagents to adjust the pH, or are there alternative methods available?"

I would greatly appreciate any insights or advice on these questions. Thank you in advance for your help.

Best regards,

I am planning to synthesize AgNP using plant extract as a bioreductor. In the green synthesis approaches of silver nanoparticles, should I focus solely on preventing the degradation temperature of reducing metabolites such as phenolic compounds?

I would greatly appreciate any insights or advice on these questions. Thank you in advance for your help.

Best regards,

I have come across suggestions for optimizing the biosynthesis of silver nanoparticles to achieve smaller particles (<100 nm), indicating that the preparation of AgNO₃ can influence the size of AgNP. What is the correct method for preparing a 1 mM AgNO₃ solution, and are there any specific considerations I should be mindful of ?

I would greatly appreciate any insights or advice on these questions. Thank you in advance for your help.

Best regards,

I am currently undertaking the synthesis of AgNP utilizing plant extract as a bioreductor. The synthesis procedure involved reacting the plant extract with 1 mM AgNO3 while optimizing the ratio. Various ratios of AgNO₃ to plant extract were explored, including 5:5, 6:4, 7:3, 8:2, and 9:1. Subsequently, UV-Vis characterization was performed to identify the ratio that yields a single peak in the range of 380-420 nm with the highest absorbance. Upon determining the optimal ratio, the synthesis was scaled up to a total volume of 250 mL. However, post-centrifugation at room temperature, only a minimal pellet was obtained, with the colloidal solution predominantly adhering to the bottom of a small 15 mL tube.

1. If I air dry the sample overnight, how should I prepare it for UV-Vis, FTIR, DLS, and SEM/TEM characterization?

2. Do I need to add a buffer to maintain sample solubility? Should the characterization be conducted immediately afterward?

3. In UV-Vis spectrophotometry, is it acceptable to check the colloidal solution before centrifugation and washing with deionized water? If I dilute the sample with a certain ratio because the crude AgNP colloidal solution is not within the range of 0.2-3, is that acceptable?

I would greatly appreciate any insights or advice on these questions. Thank you in advance for your help.

Best regards,

Hello Everyone..

I am trying to synthesise silver nanoparticles to encapsulate a chemical drug "X" using sodium citrate or NaBH4 as a reducing agent. I also have cited few publications doing the similar work. Now, I have encountered few articles where the drug "X" is used as a reducing agent to form Ag NPs.

Now, my question is kindly explain me that whether nanoformulations can encapsulate their own reducing agent?

Help me with the theory behind the answer.

Best Regards

I need refractive index of silver nanoparticles for zeta potential and zeta sizer (edited) analysis. I would be grateful if you could suggest it

Hello

I prepared silver nanoparticles by various method but every time peak disappeared, as the peak of silver nitrate salt at 300nm and it disappeared when I reduced it chemically. Now suggest me whether I put these peak as it is or I find out other method for silver nanoparticles synthesis because in literature their peak appear at 350 to 400nm .

Kindly suggest the best way.

AToI

I have prepared naked silver nanoparticles by using sodium borohydride reductant. I have dried the sol in oven but the dried particles were like a dot on glass vial and yield was too low. I have also centrifuged the solution at 5000 rpm for 20 min but get nothing. Please guide, how can i get the powdered form for XRD and SEM.

I'll be very thankful in this regard.

Hello every body,

Can you please help me

I synthesized silver nanoparticles using the green synthesis method, but its absorption peak showed 350, while it should be around 430 nm. I also observed the light conditions.

What should I do so that absorption is within the normal range?

The attached table below shows the zone of inhibition (ZOI) in mm of:

1. Rutin

2. Heliomycin

3. AgNPs(silver nanoparticles)

4. AgNPs + Rutin

5. AgNPs + Heliomycin

6. Tetracycline (positive ctrl)

Hence, my question is how to know if there's an interaction effect (additive, synergistic, or antagonistic) antimicrobial effect of AgNPs + Rutin & AgNPs + Heliomycin when referring to the table:

I am working on the biosynthesis of silver nanoparticles, before that I want to Phytochemical screening the particular plants, for analyzed the antibacterial compounds, which technique is suitable for for analysis, I mention the Thin layer chromatography (TLC) and Gas chromatography–mass spectrometry (GC-MS) techniques in my research proposal.

For Example: Nano-silver, these particles serve as antibacterial and antifungal agents for industrial purposes, in water treatment. Nanosilver destroys microbes primarily by releasing silver ions which are harmful to many microbes but if its speed is not controlled then it may cause severe damage.

I tried to make AgNP by mixing AgNO3 and 1% sodium citrate two times.

In the first trial, there was no problem to make AgNP, but in the second trial

there were some floating matters like eraser debris on the surface of the AgNO3 & Sodium citrate mixture in the beaker. Also the yield of AgNP is lower than the first trial. There was no difference between the first and second trial about the protocol.

(I used the same beaker of the first trial for the second trial after washing)

Who can teach me why the floating matters occurs sometimes when mixing AgNO3 and sodium citrate?

(Sorry for my poor english)

Has anyone used FRITSCH ANALYSETTE 22 NeXT Nano for green synthesized silver nanoparticles?

I am having trouble setting the parameters (%pump, beam obscuration, absorption index, etc) to get accurate beam obscuration and readings. I am working with solutions prepared with plant extracts and AgNO3 solutions, the UV spectra make us believe that we have good synthesis since characteristics maximums appear in the range of 420-470 nm, and a change of colours is also observed. However, when using the Fritsh equipment we are not getting a signal. At the moment we do not have other equipment to measure.

I am currently working on green synthesis of silver nanoparticles from plant extract and their antioxidant and antibacterial activity. I obtained the pellets after centrifugation and lyophilization. But when I tried to resuspend them in DMSO/Water it didn't dissolve fully. In which solvent should I dissolve the silver nanoparticle for DPPH, well diffusion and microdilution assay?

I've been synthesizing AgNP for antibacterial effects by mixing AgNO3 & sodium citrate.

After making AgNP I measured the size using DLS, the size of AgNP is about 200~400 nm.

For my experiment I expected that the size is under 100 nm, so I need to make it smaller.

So, is there any method to make it smaller?

(I heard that hydrothermal reactor can reduce the size, but I can't find any reference about reducing the AgNP size by using this)

Actually I am done biosorption assay, heavy metals removal using silver nanoparticles.

I performed FTIR analysis of silver nanoparticles before adsorption, and FTIR analysis was also done after adsorption assay.

After adsorption I got a different FTIR spectra, some peaks shifts and peak intensity differences, these could be definitely due to metal ions.

Here I am confused how to interpret results, that what happened between nanoparticles and metal ions which caused spectral changes.

Kindly guide me on this.

Thank you.

Is pH condition change after synthesis of chitosan coated silver nanoparticle?

Hello everybody.

I wondered if you have an idea that increasing the cuvette (sample) temperature in UV-vis measurement increases the general absorption of silver nanoparticles.

If yes, I would be grateful if you introduce a reference for that.

With kindest regards.

Mohammad.

In the methodology of biosynthesis of silver nanoparticles

Hello dear colleagues! I would greatly appreciate it if you could recommend a protocol for a release assay of silver nanoparticles from a hydrogel membrane. I would like to know what amount of silver nanoparticles is eluted at different time points. Is it possible to run this test with uv-vis?

is it possible for sublimation by the help of lyophilizer ? or hot air oven?

I got xrd result intensity vs two theta...now plot scarrer equation and got result 0.34 nm, is it possible?? I don't know...

Thiol-modified oligos are commonly supplied in the oxidized disulfide form by vendors. Is reducing the dithiolated oligos with TCEP or DTT to -SH compulsory before immobilizing them on Gold or Silver nanoparticles? Has anyone tried the immobilization with and without pretreatment of the oligos? Kindly share the differences in the immobilization result between the one with pretreatment and without pretreatment.

When silver nanoparticle used as test compound for cytotoxicity studies (MTT), the results showed like the compound is toxic upto a concentration, then onwards increased cell viability with increasing concentration of the drug. Is it possible?

I used untreated cotton fabric,treated fabric with silk and silver mixture,silver nano particle, silk nano particle

Here, s4b(1,2,3) are untreated cotton fabric,s3b(1,2,3) are treated fabric with silk and silver mixture,s(21,22,23) are silver nanoparticle and s(11,12,13) are silk nanoparticle

Please anyone interpret the SEM analysis result

what is purpose from adding polyvinylpyrrolidone (PVP) when doing the silver nanoparticles ?

I am a scholar and want to perform antimicrobial activity of silver nanoparticles prepared from mango plant. Do I have to perform MIC for this or antimicrobial activity can be performed directly?

We use \citep for citing multiple authors from reference bibtex. And it should come as (first author et al.,year). But for me...for some references to cite multiple authors, et al is not coming. All the authors are displaying. How can i get et al in the text?and et al shouldnot come in references.

Eg: That affects things which is described in \citep(Ahmed2020silver).

The below shows my bibtex file.

@article{ahmed2020silver,

title={Silver nanoparticles synthesized by using Bacillus cereus SZT1 ameliorated the damage of bacterial leaf blight pathogen in rice},

author={Ahmed,T and Shahid,M and Noman,M and Niazi,MBK and Mahmood,F and Manzoor,I and Zhang,Y and Li,B and Yang,Y and Yan,Chengqi},

journal={Pathogens},

volume={9},

number={3},

pages={160--178},

year={2020},

publisher={MDPI}

}

Result displayed in the generated pdf :(Ahmed,Shahid,Noman,Mahmood,Manzoor,Zhang,Yang and Yan,2020).

In xrd test I got two values like Intensity and two theta ....I calculated in scarrer equation & attached some file...Please anyone can help me and also recalculated the nanoparticle size in Nanometer...

I have synthesized Ag nanoparticles in DI water. Now I want to use the Ag nanoparticles with a flexible polymer to make a thin film. I tried centrifuging at 5000 rpm for 10 mins. However, the particles have settled down and on the tube walls.

Please help me how do I successfully obtain Ag NPs in dry form.

I have synthesized Ag-Go composite using chemical reduction method using NaBH4 as reducing agent. When I took the UV -VIS of the sample, I can observe only the characteristic peak of silver nanoparticle at around 420 nm. But, I couldn't observe any peak that determines the presence of Graphene oxide. Why is it so?

I have synthesized silver nanoparticles from leaf extract of a plant (green synthesized silver nanoparticles). I have tested its antimicrobial activity for E.coli. But, zone of inhibition was not recorded. What could be the reason ?

Does transverse and longitudinal plasmons fall under localized surface plasmons? What is the significant difference between them? At what level will this affect the fabricated silver nanoparticle based electronic devices? Is surface plasmon propagation different from transverse and longitudinal plasmons?

can i directly take SERS of the protein sample after incubating it for 24 hrs with silver nanoparticles? Or is there any other optimization protocol for taking SERS spectra of protein sample?

Silver nanoparticles can be positively, negatively or neutrally charged depending on the method of synthesis. However, I used commercial silver nitrate. How can I know the charge of silver nanoparticles?

Any idea or suggestions?

Thank you:)

I want to conduct molecular docking and dynamics of silver nanoparticle (AgNP) with protein. Is it possible to make a silver nanoparticle model using ChemDraw? Do you have any suggestions to do that? Thank you.

I have some silver nanoparticle powder that I want to convert into silver nanowires. Does anyone know how I can do this?

I have synthesized silver nanoparticles using electrochemical reduction of silver nitrate solution. I also added citric acid as a stabilizing agent. Please say how I can conclude that all my Ag+ is reduced to Ag0.

I prepared the 5 colloidal solutions of silver nanoparticles by using different concentrations of silver nitrate. I also have their UV spectroscopy results. Now I want to find their concentration. Can you please share with me the detailed method to find concentration?

I have synthesized silver nanoparticles using electrochemical reduction of silver nitrate solution. please say how I can conclude that all my Ag+ is reduced to silver nanoparticles

I've been using sodium dithionite as a reducing agent for silver nanoparticle generation.

It also appears to work for copper.

However, I am trying to cap the nanoparticles with various molecules and it occurred to me that oxidation products of the dithionite might be interfering.

Has any had any experience with using dithionite for this?

I synthesize silver nanoparticles. its liquid form and I used water. it is tough to dry and make powder form. please suggest to prepared a sample for SEM.

I am trying to synthesize drug-coated or loaded chitosan silver nanoparticles. I try reaction with 100% drug DMSO is not form.

i am working on optical properties of silver nanoparticle.