Questions related to Shrimp
Does the presence of other aquatic animal in wetland ecosystem affect the spread of parasite in the freshwater shrimp? Is it possible that the parasite of freshwater shrimp can be ingested by fish? Hoping for positive feedback. Many thanks.
What connections exist between rotifers and freshwater shrimp? and what advantages might they have for one another? Are there any studies that support the reasons?
What are the possible conditions that would have prevented the parasite from being present in freshwater shrimp? Anyone have a suggestion? I'm trying to find a rational article that will explain why freshwater shrimp don't have parasites. Your help is greatly appreciated.
I would be very glad, if you be able to point me on some research on the impact of change in mesh size in static gears on fish and invertebrate catchability. Something like “increase in mesh size by 50% will reduce catches by 30% and fish/shrimp size would increase by 10%.” Information from trawls also would be useful.
Combining feed enzymes, and minerals with probiotic microbes will affect the efficiency of microbes??
How to make the Composition by mixing the 3 of them?. (Probiotic, Feed enzymes, Minerals)
Hi all! I am looking for help on the best fix brine shrimp (Artemia salina) in 4% PFA.
Does anyone have experience with this?
Since these shrimp have an exoskeleton, how long should I fix them for at 4 degree C?
Any literature or suggestions on how to perform this fixation would be extremely helpful! Thank you in advance!
My Research Data Contains Area, Production and Productivity of Shrimp culture. I want to apply the Hazel decomposition model to my data.
Hazell’s (1982) decomposition model, which decomposed the sources of change in the average of production and change in production variance into four (4) and ten (10) components.
Many researchers have used these models for their research and published them.
Can someone explain me how to do hazel decomposition model calculations?
Would you please guide me how to go about, how to calculate the component change in mean production and component change in variance production.
Would you mind helping me develop this model, or recommending a researcher who can do it, and I will give you proper citation for it and also authorship also?
This is my mail id. firstname.lastname@example.org.
Here with iam attaching the my data set
We intend to detect WSD (white spot disease) in infected shrimps (post larva and broodstock). We need to use accurate, very sensitive, rapid detection and of course cost effective kit. So can anyone know which kit/kits are suitable for the detection? Does anyone have recommendations for that?
Thanks in advance.
Dear colleagues! We plan to isolate mitochondria from freshwater amphipods, but didn't find any methods in literature - the closest found was the method of isolation from whiteleg shrimp Litopenaeus vannamei.
The problem is - the amphipods are quite small - around 1 cm long, so it's hard to isolate the gut before mitochondria isolation.
Will it work if we use just the sample of 10 g (or is that too much?) of amphipods and blender to homogenize it in isolation medium? Or it is crucial to select only some parts - for example only the amphipods legs and antennas?
P.S.: we do not have chitinase, nor the chance to get it in time.
I would want to cultivate EHP from shrimp to study its life cycle. However, Im unsure of the protocol on cultivating EHP in shrimp cell line. Can anyone help me on this?
The results of my study on the parasitic fauna of economically important crustaceans in the Liguasan marsh are not encouraging; it seems that phytoplankton predominates over parasite in most cases. This is significant since the content of my paper may change based on my findings. Can someone suggest relevant studies on this subject?
During the examination of gills, I've been seeing the presence of eggs however it is not yet identified since I can't find any studies that have the same result. The situation of my sampling site is that their comfort room is not properly built the feces will directly go down into the water. Is it possible to detect an egg in the parasitological examination of freshwater shrimp? Can you recommend me any studies?
- size of holding tank = 41M2
- The average sieze of shrimps= 2grams
- shrimp type = Litopenaeus vannamei
What is your opinion on the ongoing discussion regarding the taxonomy of the genus Penaeus?
As someone that is not a taxonomist, when I began working with shrimp I was not aware of it and simply used Litopenaeus because it was the name that I mostly read in recent publications. Today I came upon a recent article published in Aquaculture "Making sense of the taxonomy of the most commercially important shrimps Penaeus Fabricius, 1798 s. l. (Crustacea: Decapoda: Penaeidae), a way forward" that drew my attention to it. There is also an older article by Tim Flegel that deals with this (See below). I am considering using his recommendation of placing the sub-genus in parenthesis, e.g., Penaeus (Litopenaeus) vannamei, because I find his arguments reasonable and what the Yang et al. (2023) study found, but I am concerned because it seems that the use of the sub-genera as genera is very prevalent already.
The photographs below show what I saw when conducting my undergraduate thesis. My issue is that I haven't been able to find any research that have produced results that are comparable to mine. In order for me to begin my statistics, could someone please assist me identify what I have found or confirm that they are parasites. Many thanks
Hi everyone. I'm planning on determining MP presence, size, color, shape, etc., in other words, in doing a visual sorting/characterization of MP accumulated in penaeid shrimp abdominal muscle. Nevertheless, visual sorting becomes more difficult as particle size get smaller, and is time-consuming and is more likely to fall into misidentification errors. Generally, it is recommended to do visual sorting with plastics no less than 500 microns, but I'm anticipating that any plastic embebed in the abdomen is much smaller than that. I was planning to try alcali tissue digestion with KOH and fiber glass microfilters of 2 microns of pore size, and my intention was to observe the filters under a stereoscopic microscope of a minimum of 45X of magnification. But still I'm going to obtain small plastic particles, if any (spoiler: there will be). So my question is if you have any recommendation or alternative method?... observe the filters under a fluorescent microscope using Nile red to facilitate MP discrimination? analyze another tissue? use a greater pore size filter? change the organism... or maybe it is possible to do the job. Espectroscopy methods are not allowed, since it is part of another stage of the project, I just wanna perform visual sorting/characterization.
Thank you very much for your attention.
Red cherry shrimp grow well with algae based diets. However I am confused on which commercial feed to select for feeding the cherry shrimps.
I have been using folmer primer for the amplication of CO I gene in Caridean shrimps. But even though the DNA quantity is good, I'm not getting bands in AGE after doing PCR. I tried with dilution of DNA in 1/10 and 1/20 and put it on a gradient temperature PCR at an annealing temperature range of 47-55 degrees. Can anyone help me with this problem?
I'm doing an evaluation of microplastics in several coastal species tissue samples, and what I want to know is what is the volume ratio of solvent:tissue to be used in the digestion process. The articles I read (not all literature, my bad.. my bad) are somewhat cryptic about that. They did mention volumes of solvent mixtures to then put them on tissues, but I'm interested in knowing, for instance, how much volume of KOH or H2O2 is necessary for achieve the digestion of shrimp tissue (such as the abdomen). Some papers mention 10 ml of 10%KOH but seems like is too little, and I found another that mentioned 150 ml. So, is there a precise volume? or you just simply add the solvent until covering the tissue. Or, because the incubation period, even a small volume of solvent is enough for the digestion purpose. Thanks for your time and (hopefully) answers.
In shrimp hatchery, usually water exchange takes place after animal reached postlarvae stage. So how to retain the probiotic microbiome again quickly in order to avoid pathogenic bacteria's bloom???
Suppose in Brine shrimp lethality tes of phytochemicals, no death of shrimps observed at all tested concentrations. What would be the LC50 of the sample?
Hello, I am working as a PhD student in the field of marine biology. I am preparing for a sampling procedure of live shrimps via scuba diving in a remote area for aquaculture purposes. Could someone provide me with a suitable protocol for marine habitats? Thanks.
Following haemolymp extraction from prawns/shrimps, and after mixing haemolymph with an anticoagulant solution and 4% formaldehyde, do you recommend to centrifuge sample to concentrate haemocytes at the bottom of the tube? If recommended, what are the protocols used for that centrifugation (speed and time).
And how long are the haemocytes preserved with formaldehyde? Will it be possible to recount again after some time? Considering the samples are at 4 °C.
Can anyone suggest to me any reference on the method used for the isolation of Vibrio parahaemolyticus from whiteleg shrimp (Litopenaeus vannamei)?
Mantis shrimp are known to have up to 16 different types of cones, polarized vision, and are the only animals known to detect circularly polarized light. I would be interested in hearing from anybody doing research on how their vision works and more importantly -why?
Anybody with an experience of farming of both Penaues monodon and Penaeus vannamei together. What will be the economics ?? Require expert opinions!!!
Thanks in advance.
For part of my research I am attempting to assess the abundance and diversity of crustaceans in an aquatic habitat. I intend to take picture of the specimens once collected before they are preserved and lose their colour. I mainly wanted to know if there were any specific guideline to taking taxonomic photographs of shrimp e.g. how it should positioned/oriented, should the appendages be positioned in a specific way as well?
I am going to establish a biosecured SPF black tiger shrimp hatchery. Regarding this I need a operation manual on SPF black tiger shrimp hatchery management.
My experiment is about M.rosenbergii, my shrimp lenght is about 20 mm. But I do not find out shrimp day from hatching. Please help me. Thank you for reading. Best regards.
Biofloc culture is recent promising and sustainable technology for shrimp/fish production. Using nitrification process converting waste as productive nutrients with zero water exchange.
Is it possible, biofloc culture in earthen ponds?
Is it possible, without using HDPE sheets or cement tanks?
Vertical aeration (without disturbing soil) in earthen ponds and what is the sludge impact on earthen ponds?
Is it possible to zero water exchange in earthen ponds?
The shrimps (7 specimens, 10 - 20 mm) were caught at Tista Estuary, Halden, SE Norway, close to the shore, depth 0,5 m. The salinity at the site was 4,8 ppt.
The species has all the characters of the genus Athanas, and according to Holthuis & Fransen: Costal Shrimps and Prawns, the species should be Athanas nitescens, except for one character which is not in accordance with the description: the rostrum is not straight, but pointing upward.
Thanks for help!
what can be the probable relationship of copepod and shrimps? symbiotic or parasitic ?
specially Clausidium species of copepod with ghost shrimp .
I collected this skeleton shrimp from some algae and have not been able to match it to typical mediterranean endemic and invasive species. Have considered: Caprella acanthifera, C. dilatata, C. equilibra., C. septentrionalis, C. scaura, and Paracaprella pusilla. Would love some expert opinions! He is now living in my self-sustaining jarrarium. I have more images, so just let me know if there's a specific area I could focus on.
We know that in order to grow shrimp (Black tiger shrimp/Fresh-water prawn/white leg shrimp) need to molt. When shrimp leave exoskeleton, they become very week. Since shrimp shows cannibalistic behavior, the stronger one may attack the recently molted one. So, in biofloc system, do we need to place artificial substrates to protect this problem? How can we place the substrates in biofloc tank?
I am currently working on a project using Gammarus Pulex (Amphipod). Can anybody recommend at least three solvents that are not harmful to freshwater shrimps (Gammarus pulex), it should also be a good analytical solvent and to be as little complex as possible (i.e. not to contain too much noise).
I have a project that research toxicity of PCBs 153 and 101 congeners on Macrobrachium rosenbergii (Post_larvae, 9-10 mm). But I don't have a trusted LC50 and EC50 of these chemicals on Macrobrachium rosenbergii or shrimp.
So I need your helps to write my experiment procedure.
Thanks for your helps and best regards.
Dear M. Hunte,
I'm writing for my colleague Marie ROBERT, we work for the National park of Guadeloupe (French West indies) on the rivers and the aquatic macrofauna. Since 2016, the project "Guad3E" is studiyng the environmentalDNA method to inventory fishes and shrimps of guadeloupe (http://www.guadeloupe-parcnational.fr/fr/des-actions/les-projets/projet-guad3e/congres-internationaux). During this study, we discovered the DNA of Atya lanipes (source Genbank) in the Ziotte river: a river located on the north of the island (see map attached). The taxonomic description found in the scientific paper does not give enough details to dissociate Atya lanipes from Atya innocous.
Do you have, and could you provide us some pictures of Atya lanipes to help us in the determination of these both species ? Is there any way to easily differentiate them ?
Thanks in advance,
the gut related diseases like White feaces, white muscle, white gut are doing much damage to shrimp by retarding growth and increasing FCR and eventually heavy losses. is there any medicine or cure available now apart from preventive measures?
Industrial processing of shell fish for human consumption yields around 80% by-products and only 20% edible flesh. The processing of shell fish viz., shrimps, crabs, prawns, squilla etc. results in an immense quantity of by-products which include cephalothorax, shell etc. Every year, thousands of tons of fish by-products of high nutrient content are dumped or discarded by fish processing plants throughout the world.
Traditionally isolation of chitin from crustacean shell waste consists of three basic steps: demineralization, deproteinization, and decolorization. The subsequent conversion of chitin to chitosan (deacetylation) is generally achieved by treatment with concentrated sodium hydroxide solution at 100 ºC or higher to remove some or all of acetyl group from the chitin.
Zoea 2 syndrome is currently the greatest loss of shrimp larvae across worldwide. By searching various articles and the discussions had with experts in aquaculture, the reasons behind Zoea 2 syndrome was not clear and the experts say that it may be due to the algal quality, nauplii quality and water quality of which the exact factor was not know, can anyone elaborate on the exact causative agent of zoea 2 syndrome in vannamei and the best control measures to prevent it apart form best bio-security practices would be highly appreciated and if any articles pertaining to zoea 2 syndrome in vannamei would be highly appreciated.
Thanks & Regards
Dr Vishnu Kiran Manam
Total nitrogen was calculated with Kjeldahl method, I found different equations and factors
''Total nitrogen content of shrimp waste was determined using the Kjeldahl method. Separately, pure chitin was prepared to determine its nitrogen contribution allowing to estimate the crude protein content by multiplying nitrogen content attributed to proteins by the factor of 6.25''
''Total nitrogen and chitin nitrogen were estimated by the Kjeldahl method.Corrected protein was obtained by subtracting CN from TN and multiplying by 6.25, the Kjeldahl conversion factor for meat protein, assuming that protein has 16% nitrogen.''
(TN-CN)*6.25=11.25 protein content
‘’Total nitrogen present in shrimp shell protein hydrolysate was determined using the Kjeldahl method. Total protein was estimated by multiplying total nitrogen content in chitin by the factor of 6.25.’’
TP=TNC*6.25=10.625 protein content
protein (N 4.38) according to Crisan and Sands (1978),
When protein is determined on the basis of total nitrogen content, a Kjeldahl factor of N x 4.38 (FAO, 1972) is commonly used (Levai, 1989), also on other edible fungi (Vetter and Rimoczi, 1993). The correction of the Kjeldahl factor from 6.25 to 4.38, however, is not based on strict analytical data but on common agreement.
’Protein%=(N total % - C% x N% chitin) x f
where N Kjledahl is the total nitrogen content in the sample, C chitin is the chitin content in the sample, Nchitin is the nitrogen content in the chitin at complete acetylation (6.33%), and f is the remaining nitrogen to protein conversion factor (6.25)’’
P=(3.7-C x 1.7)x 6.25
Would anyone be willing to start a collaboration by sampling freshwater atyid shrimps in Egypt, in particular in the Faiyum Oasis?
In an integrative taxonomic approach combining morphological and molecular data, this would help me to delineate species.
For a class I am looking at the effects of ocean acidification, and within this although I am finding that there are species that are both negatively (coral, oysters, etc.) affected and positively affected (blue crabs, shrimp) by ocean acidification; I want to know if there are a species that do not seem to be affected by acidification and can convert carbonic acid into bicarbonate, therefore limiting the effects and maintaining a controlled pH level.
If not, is there anything that could be created to do so, and is there any research on that?
I have a ciliate problem in my aquaculture system and would like to ID two ciliates that I believe feed on mollusk? I have photomicrographs...
Are there any ciliate experts out there who might help?
I am looking for a chemical method for Extraction of Chitin and Chitosan from shells of crab, crayfish and shrimp, and i want some suggestions for encapsulation with chitosan.Would appreciate if any one can share protocols or literature for this procedure
This query relates with countries having no marine coastal areas.Shrimp breeding facilities are usually linked with coastal line ares.
I'm performing a length-weight relationship in two populations of caridean shrimps (they are from two distinct seasonal periods), the pooled data was divided into males, females, non-ovigerous females and ovigerous females. I performed the equation and obtained the b slopes of each sub-sample analyzed, but now I want to compare those slopes to know if there is any different between them. I read that I could do an ANCOVA to compare, but I'm not sure if there is another methodology. Thank you.
We are looking for the suitability of fishing live P. longirostris for a study on the energetics of this species. We are aware of the bottlenecks of depth as a significant factor in their survival, but any onformation on fishing gears and/or techniques will be highly appreciated
How can I explain no significant difference in oxygen consumption rate, but a significant ammonia excretion reduction in shrimps exposed to different anesthetic concentrations?
Thank you in advance,
We have a shrimp sample from IAEA for proficiency test and as per the requirement of IAEA we want to identify and quantify radioactivity in that sample. We have taken a sample count for 90000 sec.
Suppose we got the gross count for K-40 (1460.822 keV) is 1.03E+04 with an area uncertainty of about 121.69.
Before the measurement of the sample, the gamma background at laboratory site was determined with an identical empty plastic container used in the sample measurement.
The background gross count for 1460.822 keV was found as 8.71E+03 with an area uncertainty of about 111.49. We can easily calculate the net count rate from the mentioned information.
But my question is how to calculate Net count rate uncertainty.
Hi. I would like to ask you.
We typically fix fish tissue with 4% PFA and 30% sugar for FISH or IHC.
Recently, however, the failure of frozen section for FISH or IHC using shrimp, a crustacean, has been continuing.
Does shrimp have to be fixed in other ways?
I have a question about cloning and gene expression of chitinase enzymes from shrimp.
Is the expression of shrimp chitinase gene in what kind of bacterial strain?
Thank you for your answers
Dear all, I am currently experimenting whiteleg shrimps in a biofloc system. After rearing the juveniles for few weeks, the water in my tanks started to have a lot of brownish foams. These foams begin to cover the water surface, obstructing my observation. I measured the water quality parameters (TAN, nitrite and nitrate) and are within the optimum range. I have read about possibility of biofloc transition that will cause the large amount of forming.
But what are the possible reasons that will cause the large foaming like this? Is it bad if it continues to bloom? Any alternative methods to reduce it?
The specimen is under the genus Heterocarpus obtained from Indian coast between the depth range of 250-350. I would like to know the species level identification, based on rostrum deformed nature character of the Heterocarpus, it is doubtful. I Kindly request to identify the species.
I have sets of data on the shrimp's weight and length measured on weekly basis. I was able to calculate the mean and SD of the shrimp weight and length for my 30 days culture. But to calculate for specific growth rate, weight gain percentage, how to calculate the SD for these parameters based on the my data? Should I calculate and tabulate these parameters in weekly basis based on the weight and length data, and from that I calculate the SD of the specific growth rate and weight gain % for 30 days culture. Need help on understanding the statistics.
Thank you very much.
By the way, I am currently using excel to measure the growth performance parameters. Please recommend any good software for ANOVA analysis, specially for growth performance parameters.
Please note that the fish size is small, so we will not enough blood for exosome isolation.