Questions related to Shoulder
I applied the real-time product on 2.5% agarose gel and only targeted the specific band, but even with changes in primer concentration, cDNA, and temperature gradient, these shoulder are still present.
The melting curve file has been uploaded.
Eri Mountbatten-O'Malley's question: "why don’t we see young people as citizens in their own right?" seems to be on more and more minds. Academics, practitioners - sometimes they are both - are adding weight to the longstanding argument for young peoples' (including children's) enfranchisement.
I have not read a convincing argument about why young people should not be enfranchised. As John Wall recently wrote: the onus should not be on young peoples and their allies to demonstrate why they should gain the vote but rather that onus should fall on the shoulders of the people who do not want to enfranchise them.
Both so very much evidence and ethics side with young peoples enfranchisement. Is this "the" suffragist movement of our times?
I am working on active targeting in nano drug delivery field. I want to have idea about width of folic acid shoulder in UV-Vis spectroscopy. Regards Nabeela Jabeen
Recently, a paragraph was forwarded and went viral online, causing great panic for a time: “Lancet, a leading medical journal, recently published an article and wrote that lumbago has become a major global challenge.” The data showed that the patients of lumbar spondylosis in China had exceeded 200 million, and the disease becomes prevalent among young people.
But why the adult labor force in China 40 years ago could carry a hundred pounds on their shoulders, but now lumbar spondylosis prevails among young people in China?
Through 6 years of unremitting efforts, I have made progress in the research on the traditional shoulder-carrying culture and spine of china, the world medical science confused the Oriental spine with the standard spine in Western medicine textbooks, yet there are stark differences between them. Research in this academic field is still blank in the world. Occasionally, the scholar (it's me) becomes the first discoverer and researcher in this field. It is the historical mission of China to sort out its national heritage, and it is the duty and obligation of every Chinese to fulfill the historical mission.
The traditional Chinese spine was born under the traditional shoulder-carrying culture of the Chinese nation, and will disappear with its demise. Today, shoulder-carrying skills can be called an intangible cultural heritage. The skill may disappear within a decade and is now on the verge of extinction. Only those elderly people have maintained the shoulder-carrying tradition, meaning that fewer and fewer people have the traditional Chinese spine. Unfortunately, the modern medical community has little or no knowledge of the structure, physiology and kinematics of the spine, leading to huge losses.
I think the study of Chinese traditional shoulder-carrying culture and spine can solve the mystery of the epidemic of lumbar disease.I have the answer, but I do not have strong scientific research ability to write it out. Time is urgent, so I put forward my opinion through the website of the researchgate hoping that more people in the world will know about this and participate in the research.
There is no standard ratio in FSW process, but i think it might be existed a logic ratio between these 4 parameters for each material.
for instance, depending on the pip outer diameter and its thickness, which are making limitation in diameter of the shoulder if extend the shoulder’s diameter to provide enough temperature due to complete plunging, extra flash will occurred.
To prevent flashes to get an optimum quality of weld, less influx will be logical decision which lead to insufficient touch and friction between the shoulder and pip.
this is a challenging!
The author's name is Gao Chengyuan
- The pole is the oldest, most important, simplest and most wonderful achievement of Chinese civilization. The Great Wall and the canal are all picked out by the shoulder pole. What about the rammed earth foundation site of the Xia and Shang in Erlitou? Only the Chinese use the pole, and the Korean culture is very close to the middle Earth, but in the old days, they all use the big basin on their heads. India and Africa are much the same.Take a comparison of two pictures (the illustration of this article is used to demonstrate the function) : the African can only use the top ten bricks, two hands to support; The Chinese migrant worker carried forty bricks on a pole and walk across the diving board. The old "razor picker" consisted of the whole barbershop apparatus, including a fire, and the picker used his free hands to rattle to attract customers.
The author is nearly 90 years old, and he is a friend of mine. I hope that some researchers can contact us, and we can exchange information together.
Is there any standard to perform landform classification based on curvature and slope using specific values for small agricultural areas (10-20ha)? For instance, to define the following hillslope positions: summit, shoulder, backslope, footslope, and toeslope. Thanks.
Hello to everyone
1- project : I am modeling fiction stir welding in direct couple analysis. this process is transient analysis. to aim this problem I utilized ANSYS mechanical apdl.I already modeled FSW using the simplified sample that is available in ANSYS 17 ( by excluding Pin and temperature dependent plasticity model ). Now by regarding a complete tool (shoulder and pin )and temperature dependent plasticity model I want to simulate FSW
2- the arisen problem: I made a log file carefully including all parameters ,loading ( plunge, dwell,welding), boundary conditions, material property etc. are required for the process. but in dwell stage after a penetration of 3.3mm some errors occur ( thermal loading and high distortion of element). it should be noted for starting of processes a penetration of 5.6mm is needed ( pin length is 5.5mm). I manipulated contact options and decrease load steps many times but the issue still remains, 3- note: some methods like NLADM( non linear adaptive meshing) suggested but this methods are capable of static analysis or we have some limitations in used element types. for me analysis type is transient and element type is solid226. solving this problem is so vital and crucial for me. any comment or suggestion about solving these arisen errors ( thermal loading and convergence)which has been attached, would be appreciated. Regards
I'm trying to know how can I investigate about the muscle functions in these three object?
To whom may have a solid background in X-ray photoelectron spectroscopy,
The technical deconvoluting way is not an issue for me, I wonder how I can decide the number of shoulder peaks (or hidden peaks underneath). For example, this old research (A.P. Grosvenor et al. / Surface Science 600 (2006) 1771–1779) tells us they deconvoluted the spectrum in 4 Ni-related peaks and 2 satellites, without any reasoning or previous step. I found many similar cases in other outstanding research, it seems they assume that most of us already understood/or agreed with the number of possible oxidation states.
I appreciate your answer in advance.
what were the main texts and scripts for knowledge transfer from Egypt, Mesopotamia, Persia, and India to Ancient Greece?
Or was it Alexander the Great?
What were unique Ancient Greek contributions to wisdom and philosophy and not standing on the shoulder of giants?
Share of creator/imitator content?
Cherish your ideas.
I read the paper "Zn-alloyed MAPbBr3 crystals with improved thermoelectric and photocatalytic properties"
MAPbBr3 microcrystal was fabricated using hydrothermal method
"MAPbBr3 precursors were transferred to stainless-steel Teflon-lined autoclave at 160oC for 6 h. It was cooled down to RT and dried in a vacuum oven at 50oC for 12 h."
I'm wondering why this MAPbBr3 showed (100) XRD shoulder peaks although they insisted tetragonal and cubic phase coexist at RT.
As far as I know MAPbBr3 only exist in stable Cubic phase at RT not tetragonal.
Are these shoulder peaks related to the unequilibrium thermodynamics during solvothermal methods?
How can we explain these peaks?
I also checked other MAPbBr3 papers, fabricated by similar solvothermal methods and they just showed clear cubic xrd peaks.
I uploaded XRD peaks for MAPbBr3 paper and related references also.
The uv spectrum of beta carotene have a shoulder band close to maximum absorption. what does it indicates?
In my last research, I investigate whether patients with musculoskeletal disorders had increased susceptibility to SARS-CoV2 infection or developed more severe forms of COVID-19; as well as whether COVID-19 affected the underlying disease.
Results showed that the frequency of COVID-19 was low and statistically nonsignificant, but that led to a worsening of the underlying disease.
What are your clinical impressions, ie do you have similar research results?
I wanted to ask your inputs/ideas for a problem we are currently encountering in liquid chromatography.
We have developed a method for the analysis of a peptide at 1 mg/mL having a size of about 40 amino acids. The method is quite conventional, with the use of a Waters BEH C18 peptide column, together with a mobile phase composed of ACN and water + 0.1%TFA in both solvents. Obviously, we are working under gradient conditions.
We have performed more than 500 injections on the column without any issue. However, after this number of injections, we have begun to observe a strange peak distortion. Therefore, we believed that the column was too old, and we decided to order a new one.
With the new column, we have performed less than 20 injections of the sample without special problem, but after these few injections, the problem came back and similar peak distortion was observed. You can see the enclosed picture to have an idea of the very strange peak distortion observed.
Therefore, in order to ensure that there was no issue with the column batch, we have re-ordered a new column of the same type, but the problem came back after only 10 injections.
To be honest, the peak shape looks very special, and to the best of my knowledge, I have scarcely seen such shouldered peak shapes in liquid chromatography.
If you have already observed such a behaviour during your career or if you have an idea of what could be the reason for this peak distortion, please don’t hesitate to share your thoughts.
It is sometimes great to think outside the box, and this is why I have posted this message here.
Many thanks in advance for your help.
It is being observed that some surgeons prefer to do reverse shoulder replacement rather than attempting a rotator cuff repair in the elderly patients giving justification that the results of rotator cuff repair are not 100%.
Is it scientifically correct to offer reverse shoulder arthroplasty for roator cuff tear?
I am trying to investigate the coherence between two shoulder muscles at 0 - 90Hz. Precious studies used various cuff-off for their band pass filter, e.g. 1–400 Hz(Chen, 2012), 5-300Hz(Boonstra,2008), 3-1000Hz (Kattla, 2010). How do I determine the most appropriate filter for my analysis?
Also, we measured both surface and intramuscular EMG, but some intramuscular EMG signal are not good. Is it acceptable if I downsample the intramuscular EMG of one muscle, and calculate the coherence between it and the surface EMG of another muscle?
The material is a biopolymer (biomass) The broad peak I think is an OH peak suggesting an intermolecular hydrogen bonding interaction normally observed in homopolymers and copolymers. but the shoulder around 3249 I got no explanation.
Hi all - I am testing some probe/primers assays to see if I can multiplex one of my ref gene (PPIA) with a target gene '(Tas1R2).
This is a typical amplification curve obtained - I have diluted the ref gene assay 1:2 to reduce possible inhibitory competition with target gene.
Still the target gene multiplex amplification curve show a shoulder that I do not see in the singleplex amplification curve. Any idea of what causes this ? Dimers between assays ?
Many thanks for your suggestions !
We have been consistently observing shoulders in FCS correlation curves on monodisperse and stable solutions of nanoparticles. We have shielded detectors from stray light and the microscopes are on optical tables that should dampen vibrations. Thought at first it might be due to a 60 Hz wall power fluctuation but even on extra-grounded outlets we see this in the curves. Also tried adjusting the sample slide to minimize any reflections that may be present in the chamber slides.
Thoughts on what this is / how to prevent it?
I am using a HPGe detector to measure the activity of a neutron-activated foil sample (InAl), which can be used in neutron spectrum unfolding.
A problem showed up is that the gamma spectrum recoreded by HPGe now shows full peaks with a "shoulder" on high energy side, as shown in the attached figure labeled with In-115(n,gamma)In-116m.
Other irradiated samples are meausred using this HPGe, but the aforementioned phenomenon in gamma spectrum doesn't occur, as shown in the attached figure labeled with La-139(n,gamma)La-140.
My collegue had suggested a possible cause that the unstable voltage supply during the measurement. But it has been excluded after the installation of uninterruptible power supply.
What will be the cause of the "shoulder" in the full-energy peak of certain sample? And, how to cope with it?
Editted at 2021-May-27 19:51
Dear Dr. Gerhard Martens,
Here is the complete spectrum and its .Spe file. The decay gamma of In-116m is specified in red with corresponding annotation (fitted energy).
Also specified on the spectrum are the detector live time, real time and dead time. The Gross/Net count rate are 86.87 / 77.26 cps for the 1292 keV peak.
The lower level discrimination is set 50 which equals to 17.33 keV. However, the HPGe recorded spectrum starts from channel 132 (45.74 keV) when measuring the In-116m foil sample.
The set-up is remained the same when measuring different samples. None of the collimator, shielings and distances are changed.
The detector model is Canberra GC2520 coaxial HPGe.
I aim to extract the value of valence band maximum of XPS spectra. Some papers suggest to extract the value by taking the intersection value of conventional linear extrapolation and the baseline. Besides, there is also another possible solution, i.e. by deconvoluting the tail feature with several small width gaussian peaks. I am done with the linear extrapolation and I would like to compare them with deconvolution method. I have tried that with origin by using multiple peaks analyzer and picked several points to be deconvoluted, however, origin did not show a good deconvolution (no gaussian peaks were observed).
Regarding to that issue, does any one have any recommendation out of origin? or perhaps still with origin but different menu option?
Thank you very much in advance.
In Ti 2p spectrum I have an intense Ti 2p 3/2 peak at 458.5 eV (corresponding to Ti4+) with additional shoulder peak at higher binding eneregy, I couldnot consider it as Ti3+ satellite because there is no shouldering at lower binding energy (which might be the actual Ti3+ peak). Please help me to interpret this.
I am using genomic DNA from sperm as the template, try to do sex-ratio relative quantification, when I ran the qPCR, it shows the shoulder in the melt curve, When I ran a gel for the qPCR products, it shows one band, but above the bands, there are some none specific thing shown up.
I am using a autosomal primer for reference gene, but either using autosomal primer or GAPDH, it gives me the shoulder in the melt curve.
1) the primers are optimized for this PCR 2) there is no primer dimers.
Can this be a problem when running the gDNA as template? Or using ROX and SYBR will give the problem?
Is this a good way to do the sex-ratio determination? When I did the calibration curve, more input of my template, did not increase the CT value (improve the CT value).
I need pure hexahedral mesh for complex geometries of human body parts such as human skull, femur, shoulder etc. I have made an attempt with ANSYS ICEM and IA-FEMesh softwares using multi-blocking techniques, but I am unable to produce Hex mesh. I am attaching snaps of geometries alongwith.
Can anyone have an idea how to generate such mesh on complex geometries?
Currently we are doing a project (metanalysis) in Rotator cuff repair and multiple authors have used different scoring system. Is there any way we compare the outcomes to determine which intervention is better than others?or way of reporting these outcomes
This is a question asked by a reviewer. But I am not good at FSW.
The original question is : Was tool tilt an important factor of your study? If not, how did you overcome shoulder plunge ?
In my study, the tilt angle was 0° during the welding process. But I have no idea how to response to his question. If any of you know anything about it, please tell me. Thank you very much.
Increases or Decreases The Grain Size, Tensile Strength and Microhardness with Increase in Shoulder Diameter.
I've recently used a pure spectra plate from Thermo to calibrate my QPCR. Upon review of the raw data, I noticed that the SYBR spectra had 2 peaks (well a peak and a shoulder peak). Is this typical? The 'shoulder' peak falls in the range of another dye so would this affect quantification of other dyes if multiplexed?
Thanks in advance
ps - picture of spectra shown
Dear RG community!
I am obtaining very weird peaks on the XRD pattern. There is a shoulder on the left side of the peak looking like a step. Arrow on the picture. It is about 2-3% of the Ka1 peak intensity. It appears before every peak with constant 2-3% of relative intensity on the constant distance. And I have no idea what it could be. It doesn't fit with any known Cu Ka satellite spectrum. Doesn't match with potential W emission from the tube.
This is perfectly flat LaB6 powder standard confirmed on the other diffractometer. Al2O3 standard gives the same artifact. The XRD is Bruker D8. Optics is in reflection geometry with copper X-ray tube, no monochromator, 0.2 mm motorized divergence slit, incident and receiving 2.5 degree Soller slits, Ni filter, PSD detector.
Any ideas what is the origin of this shoulder? Or what more important, how to eliminate it?
-The question is clear enough.
- I would like to follow that research project.
- Thanks (in advance) for all added replies. Marc.
Discoverer of the period-luminosity law in stellar distance measurement, she was the giant the likes of Hertzsprung, Russell, Shapley, and then eventually, Hubble, shoulders they stood on. For once the idea and natural observation matched with some numerical entity, the competitive numbers people built it out. I'd like more data - if available - relative to her personal life.
Recently, I changed a gas cell of my FT-IR spectrometer (MIDAC), and background spectrums collected from the new cell are a little bit strange.
What is the peak at 3200 cm-1 in the singlebeam spectra?
And why is the shoulder peak at 2200~2000 cm-1 too rounded?
Is there any problem with my new cell such as contamination or misalignment?
Or, is it okay to use this?
I was working on a pavement model in ANSYS containing asphalt bottom layer with concrete slab panels on top of it. The slabs are tied to an asphalt shoulder at one side as well. The pavement is sitting on an elastic foundation and is subjected to wheel loads. After meshing has been done, I was trying to connect the adjacent slabs for load transfer between them, with springs in longitudinal and transverse direction in ANSYS. I need some idea on how can I do that so that the rigid body movement is restrained effectively when subjected to loads. I am attaching a screenshot of my model for your kind consideration.
I performed a qRT-PCR reaction for the CDC20 gene on drug-treated cells and non-drug treated cells and obtained these melting plots and I am just wondering how to explain the shoulders on the end. Would these be indicative of non-specific primer binding? And if so, could that have to do with the volume of primer added (a pipetting issue)? Not everyone in this laboratory course had the same issue, and I am trying to explain my results
I am working on various trimetallic catalysts of Pd and two other metals for ethanol oxidation.
All of them have resulted in one metallic phase upon analysis by X-ray diffraction. That means no individual metal peaks are separate from one another.
However, I, some times, see overlapping peaks with one main peak and another shoulder peak. I think this is not the same thing as the asymmetric peaks that is sometimes found in XRD even in one metal system. I am saying this because I also prepare each metal individually and compare the trimeallic peaks with single metal ones.
For example, in the attached graph is the (111) peak of trimetallic PdRhIr vs single Pd, Ir, and Rh. You can see the main peak in the trimetallic is close to that of single Pd while the shoulder is close to that of Ir. This drives another question about how to deconvolute those peaks? I have tried originlap using both Peak Analyser and Multipeak but both have not given a satisfying result.
The main question, however, having those overlapping peaks; how could I understand them and evaluate the alloy formation potential of the three metals?
Any idea or suggestion will be deeply appreciated.
Have a great day!
I have prepared libraries for RNA-seq using the Takara SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian. The input RNA was extracted from FFPE tissue. Some of the final libraries have a shoulder or peak at around 160bp, as shown in the Bioanalyzer trace I have attached. I was wondering if anyone has had a similar issue, or could advise on the cause of this peak? Could it be due to adapter-dimers?
The compound is isolated from a plant extract and there are two possible positional isomers. Their peaks are very close that sometimes it looks like a single peak with a small shoulder. I have tried separating using binary solvent systems ( Acetonitrile in water, acetonitrile in methanol). The elution happens at higher percentage of acetonitrile.
Will three solvent systems of the above mentioned solvents work? Or could someone suggest me a better alternative?
I need to record the head and shoulders of a person speaking, and then reconstruct that person in 3D, so that it can be played back statically (not real-time) in a virtual environment.
I am interested in any software or methods there are out there for reconstruction of a recording in medium to high resolution.
I am trying to synthesize ultra small 2nm gold nanoparticles by NaBH4 reduction (Brust Method). What should be the characteristic UV-Vis spectrum of 2nm size gold nanoparticles. Should we or not see a shoulder or hump at 510-520nm ? I am not seeing a shoulder at 510-520nm. I am attaching the image of spectrum for your reference. What does it say about the quality of the nanoparticles ? I have repeated three times and the spectra remains the same for all the batches.
As a P.T., focused and interested on orthopedics (such as, back, knee, shoulder, etc., pain and dysfunctions), my main concern is: how cognition and the capacity to search and obtain information, and convert it to knowledge, can affect our patients pain and rehabilitation, specially in low income areas?
Thinking about focusing on (regarding future researches):
- Health literacy
- Information/knowledge sharing
- Group therapy intervention/prevention
Are those topics of interest? If "yes", which groups and researchers should i dig deeper to get the foundations?
Any suggestions are more than welcome
As seen on the attached image, we intended to produce Au nanobricks by EBL. The resist was PMMA 120 nm, metallization parameters are: Cr 0.5 nm, Au 40 nm. The SEM image of the produced nanobricks reveals some rim or shoulder at the edges of the bricks, which appear as brigth lines around them on the SEM image. AFM measurement approved the height difference at the edges. Does anyone have an idea how to avoid this effect and create flat surface bricks?
Is solar geoengineering frightening science fiction or bona fide scientific research that someday might provide the tools for staving off catastrophic climate change? Does humanity still have a choice between such extreme and risky schemes and reduction of emission? Are we willing to shoulder the economic burden now, or are we going to wait until we have no alternative to such strategies?
I am getting crazy about this. I am having less than perfect melting curves, with different primers. The funny thing is that while primers give a main peak at different temperatures, as it is to be expected, the shoulders associated to the main peaks appear always at the same temperature, around 71. I tried with a second different master-mix, and I got even taller shoulders, still melting at a constant temperature, this time always at 76. NTC are clear. Gel 2% does not shows any unspecific band, but amplicon is 74, so it could be difficult to resolve 2 distinct bands.
- 375.19 KBRPLP0_65C_annealingT_fluorescence curve_gradient.png
- 219.94 KBEfficiency.png
- 965.75 KBShoulder.png
- 233.15 KBRPLP0_Melt curve_Dilution Series.png
- 213.89 KBRPLP0_Melt Peak_Dilution Series.png
- 188.68 KBRPLP0_NTC_Melt curve_dilution series.png
- 161.62 KBRPLP0_54C_annealingT_fluorescence curve_gradient.png
- 366.84 KBRPLP0_NTC_Melt peak_dilution series.png
- 132.41 KBRPLP0_54C_annealingT_Melt curve.png
- 180.73 KBRPLP0_54C_annealingT_Melt Peak.png
- 167.98 KBRPLP0_65C_annealingT_Melt curve.png
- 207.78 KBRPLP0_65C_annealingT_Melt Peak.png
- 179.58 KBRPLP0_NTC_Melt curve_gradient.png
- 126.49 KBRPLP0_NTC_Melt peak_gradient.png
What is the reason to get 5-fluorouracil peak shouldering by using C18 column with 0.02 % aqueous orthophosphoric acid? When we take standard plot we got good peaks. Further, when we take accuracy and precision, it started giving peak shouldering. We washed column very well with 0.2mL/min flow rate at 20:80 ACN:WATER ratio and then with 35:65 ACN:WATER ratio. Please suggest us to avoid shouldering of peak.
In shoulder press and lats pulldown there is the "breaking the bar" principle to limit injury. Is this correct, and/or are there other options?
This to create external rotation.
Is there any other aspect opposed to breaking the bar? Maybe single arm movements? Seems so difficult to "break the bar".
Fracture dislocation of shoulder is challenging case for novice and veterans alike. Inviting tips, tricks and valuable opinions for one such case with no associated injuries.
What are the social/cultural factors that influence compassion fatigue (not burnout)? Any writing specific on that? We often tend to put all the responsibilities of compassion fatigue on the worker’s shoulders and neglect the social part of it. I'd like to have more information about that. Any readings, videos, etc. Thanks
I am actually working on a Master dissertation about kinematics applied to a human body, and I would like to know if one of you knows where I could find the average rotation values of the body joints such as :
The ankle foot joint,
The knee leg joint,
The pelvis leg joint,
The shoulder arm joint,
The shoulder knee joint,
The wrist hand joint,
which percentage count as an anterior-posterior shoulder imbalance?
I measure the strength of flexion, extension, abduction, adduction, horizontal abduction and horizontal adduction with hand-held dynamometers.
I want to evaluate their anterior and posterior shoulder imbalance.
So what is the number for the normal shoulder?