Science topic

Sex Ratio - Science topic

The number of males per 100 females.
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When to breed buffaloes to produce more female calves? ज्यादा कटड़ी पैदा करने के लिए भैंसों में प्रजनन कब कराएं? https://azad-azadindia.blogspot.com/2023/12/when-to-breed-buffaloes-to-produce-more.html
After breeding your buffalo, you wait about 10 months (305-320 days) to see whether your buffalo calved a male or female calf. However, data analysis revealed that you may enhance the chances of having more female calves by timing the breeding of your buffaloes. After analyzing buffalo birth data retrieved from Annual reports of ICAR-Central Institute for Research on Buffalo (CIRB), Hissar (2019-2022, four years), it was interesting to note that the probability of birth of female Murrah buffalo calves was twice (Odds Ratio >2; CI95, 1.02-2.290) than the birth of male calves in July and August. This means you should breed your Murrah buffalo in October and November to have more female calves. At Nabha, Punjab Centre of CIRB Nili-Ravi buffalo breeding is done. The birth data of Nili-Ravi buffalo also indicated two months with much higher possibility of birth of female calves (Odds ratio 4.5 in April, CI99, 1.02-19.5; Odds ratio 2.3 in September, CI99, 1.04-5.08) than male calves, that means you should breed Nili-Ravi buffalo either in July (for calving in April) or in December (for calving in September). This analysis is based on four-year data only (2019 to 2022), in the period a total of 632 Murrah calves and 532 Nili-Ravi calves were borne. The bigger data analysis may yield better statistics and may guide the buffalo farmers to breed the buffaloes profitably. The data needs to be handled carefully as if the climate of the month of breeding may affect the sex ratio then certainly, the best months of breeding may be different in different regions of the country. Therefore, calving data at different breeding centers needs to be relooked for the benefit of livestock farmers.
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Modulating the sex ratio in buffalo through the selection of breeding time (period) is a practice commonly employed in animal husbandry. Estrus synchronization and timed mating techniques are utilized to coordinate the breeding cycles of female buffaloes, facilitating more precise control over the timing of pregnancies. While this approach may influence the overall sex ratio of the offspring, it is essential to note that biological factors, genetics, and environmental conditions also play significant roles.
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Especially in favor of females in this case.
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In reviewing research on Australian species (Hyriidae), I’ve encountered many examples where sex ratios are clearly biased towards males or females. These often are large samples, numbering in the hundreds, and most of the biases are statistically significant. I know that age and population density are implicated in research on species of Margaritiferidae and Unionidae, and that there may be differential mortality associated with dissolved oxygen levels (in that situation, brooding females are more vulnerable to hypoxia than males). The incidence of hermaphrodites may also need to be factored in.
In our Australian examples, the biases for any one species may differ between different times or places. There is little possibility of biased sampling, as these species are not sexually dimorphic.
Is anyone aware of published studies that shed more light on these seemingly haphazard shifts in favour of one sex or the other?  
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Great question! I have also observed this in freshwater mussels, and hypothesise that it may be due to castrating parasites in our recent article here:
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Hello!
A question. Does anyone happen to know of any studies on the influence of surrounding land use (natural habitat, mass-flowering crops, other agriculture, etc.) on the sex ratio of the offspring produced by solitary bees (possibly specifically mason bees but not necessarily)?
To date, I have only found studies that indicate that larger individuals produce more females and that the proportion of females decreases through the season.
Thanks in advance!
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I would like to determine live sex ratio of sperm cells in ejaculated semen samples. I wonder whether staining with Hoechst 33342 and propidium iodide and flow cytometric analysis has enough accuracy and resolution to differentiate and quantity the live X- and Y-bearing spermatozoa. Does it need to be done by a cell sorter machine or could be done by a regular flow cytometer which is able to read both stains?
Thank you in advance.
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Yes - it's done comercially to produce sex sorted semen, largely in cattle. This process uses sorters, but if you're just after characterisation rather than collection of separated populations, a regular analytical flow would work.
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I am using genomic DNA from sperm as the template, try to do sex-ratio relative quantification, when I ran the qPCR, it shows the shoulder in the melt curve, When I ran a gel for the qPCR products, it shows one band, but above the bands, there are some none specific thing shown up.
I am using a autosomal primer for reference gene, but either using autosomal primer or GAPDH, it gives me the shoulder in the melt curve.
1) the primers are optimized for this PCR 2) there is no primer dimers.
Can this be a problem when running the gDNA as template? Or using ROX and SYBR will give the problem?
Is this a good way to do the sex-ratio determination? When I did the calibration curve, more input of my template, did not increase the CT value (improve the CT value).
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Hi Delong, a substantial shoulder peak reflects the presence of low complexity regions in amplicon, which causes non-uniform melting, but it could also be due to longer amplicons, resulted by less specific primers as now it has multiple melting domains with varying GC content and hence tend to dissociate at different Tms, giving rise to shoulder peak of varying heights. So primer specificity and optimal primer concentration can solve this problem.
Regards.....
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I want to employ Occupancy and Detection probabilitiy estimates for a large vertebrate species. Do you recommend using and running different models with presence-absence matrices by sex (i have robust data on the sex ratio in my study population), or using sex as a covariate (i.e. using the typical species presence-absence matrix) of occupancy/ detection? The rest of the covariates will be from the habitat type and the species community at my study sites.
Thanks.
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Yes, sex is a common covariate used in occupancy models. If you haven't used it already, I would highly recommend the "secr" package in R. You can then compile a capture history element and combine it with covariates to run your models and analyses.
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According to the 2011 census of India the socio-economic, political and educationally developed districts of Odisha are found huge differences of sex ratio.
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Hampton Gaddy
Thank you for your response.
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India's sex ratio (about 1070 males per 1,000 females) is much higher than the global sex ratio (about 1012 males per 1,000 females). What may be its possible reasons? In India, only in Kerala, with large Christian population, females are more than males (about 923 males per 1,000 females). What may be the reason of this exceptional ratio?
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Kerala has less Female foeticide and maternal mortality ratio compared other states
Maternal Mortality Ratio
Female foeticide in India
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I am currently planning to work on the Study of breeding biology of Glossogobius guris (Freshwater Goby) in particular reservoir (Rajdhala Beel, NetraKona) in Bangladesh for my MS thesis. I am interested to know how this study of breeding biology of Freshwater Goby in this reservoir can have socio-economic contribution. I will determine the GSI, Fecundity and Gonadal Histology, sex ratios, etc. of the particular population from the samples . Some past studies demonstrated that this species has good abundance in the reservoir. But no one worked on the breeding biology of this species in this area. How I can explain the socio-economic contributions of this study? I have already made some points of myself but additionally I need some strong points from the experts to make the objectives of the study more acceptable and more convenient in terms of benefit of people and the fisheries sector. Thanks in advanced for any kind of response anyone might provide.
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Any new observation may make species management more convenient.
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In general, sex ratio is expressed as the ratio of males to females in a population, ie number of males/number of females (*100). However, when the number of females in (sub)population is 0 (zero), how to express the sex ratio?
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In fact, the term "sex-ratio" is misleading, and the formula "males/females" should be avoided. Only a minority of authors use this formula.
Practically, most authors use the formula "males/(males+females)". This can be expressed as a probability (range: 0-1) or as a percentage (*100). It should be properly called "male proportion" but the (misleading) term "sex ratio" is traditional.
Best,
L
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article is quite interesting, but in my colony tall mothers have more male children
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Why sex ratio studies in wild natural population are limited?
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Hello Benoy;
     For several years I studied a small population of Croton californica which is facultatively dioecious. It is a short-lived perennial that occurs in Coastal Sage Scrub communities in the Mediterranean climeat of Southern California.
     Individually marked plants may change sex from one year to the next. Females outnumber males in high-quality microhabitats and they shift to male-dominated groups in dry years. As Pegman noted the cost of being female is higher than being male. It is also true that being a member of the rarer sex is a reproductive advantage.
     Thus, the answer to your question depends on the local conditions at the time the costs and sex ratios are assessed by the plants.
     Long-lived perennials probably sense the local conditions of their microhabitats differently from Croton and from annual plants.
     The problem is a wonderfully interesting one. It is certainly worth your effort.
Best regards, Jim des Lauriers
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We are working on gene expression in chicken shell gland and would like to assess how much of expression may occurred in the erythrocyte and non-erythrocyte. Therefore, a rough proportion would provide us a clue. Answers are very much appreciated.
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Hello
You can visit this website & contact with the Poultry Cooperative Research Centre  - university of New England , may help you .
Good luck
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guard family, bitter gourd, monoecious, gynoecious line, sex forms and sex ratio
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Please go through  the articles
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Since 4 years, our association studies the demography of a European pond turtle population (Emys orbicularis), located on a wet area (about 7 ha) in southeastern France. This population is totally isolated. There is no road nearby, not limiting (apparent) factors that may cause high adult mortality. Laying areas are located near water areas in relatively well preserved terrestrial habitats. However, a significant reduction of the water surface is observed.
We found a growing imbalance in sex ratio, a very low survival and a large decline in the number of breeding males over time.
Do you know of a similar case?
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IF there are any ways to reduce the bias of the traps by using different collection methods that might provide a different sex ratio. Try using different bait types (or rotating bait types), more intensive sampling, drift fences (fike-nets) into the traps, use of decoys, etc. 
What was your recapture rate? If your male recapture rates are low, you may just be sampling them poorly. 
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I did a statistical analysis by spss the chi -square test for the sex ratio I found values X2 = 856,717a Is it normal?
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What means "normal"?
Under the null hypothesis this value has a chi-squared distribution. This distribution is qualified by the "degrees of freedom" which in turn follows from the dimension of your contingency table. For rather small tables (the usual case), such a large value would be extremely unexpected under the null hypothesis.
NB: Since this value depends on the sample size, it does not tell you if the sex ratios are relevantly different. You can get such a result with large sample sizes even for tiny and irrelevant differences.
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I'm working on a database of patients (all diagnosed for cancer)  included in a clinical trial.  I'm trying to perform a comparison between those patients and patients not included in clinical trials to be treated.
In general population you can easily calculate the sex ratio of patient in the dataset by using incidence tables (to take into account age and sex repartition in general population).
But for a clinical trial, i don't know how i can perform a such thing.
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There is no exact formula to calculate the sex ratio of the patients included in a sample for a clinical trial, in a simple way one could say that if the study population the proportion of men and women is similar, for example 50% 50%, and the rate of morbidity (incidence) by sex and age group is not known, you can use the tables of incidence of the general population.
But if the cancer has an incidence (rate, frequency) higher in women (including breast) or men (such as prostate), or higher in children (renal Wilms tumor) must be set the number according to the frequency of cancer by sex and age group.
The cancer statistics (tables by type of cancer, sex and age distribution) of your country, or the National Institutes of Health US, you may find useful.
regards
Dr. Jose Luis Garcia Vigil
PS: If the sample (M) under consideration is a "captive universe," no need to calculate the size of M. It will only be necessary to make the distribution by sex, age and type of cancer (according to tables frequency). In addition, the number of patients remaining in each sub-group or cell, are sufficient to implement appropriate sample size (small or large) and variable (parametric or non-parametric) statistical tests.
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i have conducted an experiment aimed at investigating the effect of aloe on sex ratio in tilapia. I found out that aloe at low inclusion level presented a significant difference compared to the control, the following inclusion levels did not present any significant difference when compared to aloe unsupplemented fish, however, significant different was again present at the highest dosage. How do i explain this pattern of data in this field. literatures are also welcome.
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Yes, additives can have different effects at  different inclusion levels. I think it might be related to receptor saturation (when talking  about organ response) and effects on intestinal microbiota (when used as a feed additive for improving growth)
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It is proved that stopping rule behaviour does not have an effect on sex ratio at birth but it is still believed that it has effect on sex ratio at last birth. How to correct the sex ratio at last birth? What are the policy options? Just focusing on sex selective abortions through legal means may not help the developing countries like India? What are alternative options? Will correcting sex ratio of last birth contribute to balance the sex ratio? The last birth comprising 20% to 30% of total births in a country with TFR of 2.6, so there is a need to encourage the parents that even they can stop the family size with a female child. What kind of incentives need to be announced for them? India is already offering conditional cash transfer but not exactly to the girl child who is last birth of the parents. Is it acceptable if the government offers incentives for parents who have a female child as last birth with parents having only two children?
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It is dependent to ones norms and the State policies pl.
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In other words, it is possible that the sex ratio of one given insect varies from year to year with light trapping?
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Based on my personal 1,176,000 light trap hours of operation here in Louisiana, USA, you cannot in any way base the actual natural sex ratio of any moth species based upon light trap captures.  There are some species of moths which I capture 100 to 200 males for every single female captured by light traps.  Certain moth species are more or less attracted to UV light and likewise some sexes are more or less attracted to light.
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There are many references about bias in sex ratio in chicken , but no one performed direct selection on the trait , I designed my study to do selection on dam for this traits , but I need references about previous studies to avoid any replicate mistakes 
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hi dr khalid,
pl. follow population ganetics written by cavalli sforza and bodmer and /or genes in population by spiess for selection in animals and calculations
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What are the implications of biased sex ratios in hummingbirds? Do we have 1:1 sex ratio? Is that the norm? Or does it vary with the intensity of sexual dimorphism? Any good references on this question?
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Hi Mala, Thank you for your quick response and references, all very useful. I really like the field guide for hummingbirds. All the best, GA
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Dear Colleagues
What is the sex ratio in carabids?
As you all know pitfall trapping gives a bias estimate of abundance due to various reasons. Therefore higher abundance of males or females in the trap does not have to relate to their abundance. Do you know sex ratio of ground beetle populations obtained with more reliable methods than Barbet traps? Do you have some unpublished data on this topic?
With kind regards
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Hi Marcin
We did some hand sampling in alluvial areas of Aare and Bünz. At each site we have cought Carabids in fourty parcels three time periods spring, summer, late summer, 20 minutes per parcel and time period.  See below the pooled species and the sex ratios. I will not test it yet but I guess the results endorse your 1:1 sex ratio for most species and the total too.
 
 
 
Art (lateinisch)
Male
Female
Acupalpus meridianus
1
2
Agonum micans 
6
8
Agonum muelleri 
12
19
Agonum sexpunctatum 
 
1
Agonum viduum 
2
4
Amara aenea 
12
15
Amara familiaris
 
1
Amara ovata 
5
5
Amara schimperi 
4
2
Amara similata 
3
1
Anchomenus dorsalis 
4
6
Anisodactylus signatus
3
1
Anisodactylus signatus 
4
3
Asaphidion pallipes
1
1
Badister lacertosus
 
1
Bembidion articulatum 
 
2
Bembidion ascendens 
7
20
Bembidion atrocaeruleum 
78
63
Bembidion decoratum 
1
1
Bembidion decorum 
52
46
Bembidion fasciolatum 
14
21
Bembidion femoratum 
46
44
Bembidion genei illigeri 
1
 
Bembidion lampros
3
2
Bembidion milleri 
 
1
Bembidion prasinum 
12
6
Bembidion properans  
9
5
Bembidion pseudascendens 
9
12
Bembidion punctulatum 
22
19
Bembidion pygmaeum 
1
 
Bembidion quadrimaculatum 
1
1
Bembidion schueppeli 
1
 
Bembidion semipunctatum 
1
2
Bembidion testaceum 
31
25
Bembidion tetracolum 
47
63
Bembidion tibiale 
1
 
Bembidion varicolor 
5
3
Brachinus explodens 
3
 
Bradycellus verbasci
 
1
Carabus granulatus 
 
1
Chlaenius tibialis 
8
7
Chlaenius vestitus 
4
5
Clivina collaris 
9
5
Clivina fossor 
2
2
Demetrias monostigma 
1
 
Diachromus germanus 
9
10
Elaphrus aureus
 
3
Harpalus affinis 
35
24
Harpalus distinguendus 
14
8
Harpalus luteicornis
 
1
Harpalus progrediens
1
 
Harpalus rubripes    
3
3
Harpalus rufipes 
4
8
Harpalus signaticornis 
1
1
Lionychus quadrillum 
41
42
Loricera pilicornis
4
2
Nebria brevicollis 
2
6
Nebria picicornis 
1
5
Notiophilus palustris
1
1
Oodes helopioides
 
1
Ophonus ardosiacus
3
2
Ophonus azureus 
9
6
Ophonus puncticeps
2
 
Oxypselaphus obscurus
1
 
Panagaeus cruxmajor 
2
2
Paranchus albipes 
30
25
Parophonus maculicornis
1
 
Patrobus atrorufus 
2
2
Platynus assimilis 
4
3
Poecilus cupreus 
8
13
Pterostichus anthracinus 
1
1
Pterostichus melanarius
1
1
Pterostichus nigrita
 
1
Pterostichus vernalis 
 
1
Stenolophus teutonus
8
8
T. parvula
4
4
Tachys bistriatus 
1
 
Tachys micros 
4
4
Tachyura quadrisignata 
59
67
Tachyura sexstriata
3
3
Thalassophilus longicornis
1
 
Trechus obtusus 
1
2
Trechus quadristriatus 
2
2
Trechus secalis 
1
2
Total
690
691
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Taking several confounding factors, such as residence, SES, mothers age, populations, inbreeding etc how can we get an adjusted value and risk analysis (odds ratio). Which software is best for such analysis?
The reproductive features are: Fertility, mortality, sex ratio, selection intensity. For each of the reproductive feature please suggest an appropriate statistical model. 
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Hi. There are free and for purchasing options. Among the free options, I suggest you EpiInfo from www.cdc.gov it could be easy for you. R is a powerful free software, but it is complex. Among purchasing options, I think Stata is a balanced option between performance and cost. Best regards.
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What are the list of statistical tests we can do for Sex Ratio, to know whether the sex ratio is significant or not in Tribal and in Non tribal
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Under what circumstances does a female skewed sex-ratio in a population become desirable or detrimental to the local population of a species?
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Hi Azubuike,
The first thing that comes to mind following your previous comment is the skew in sex ratios in turtle and crocodile hatchlings in response to increased environmental temperatures. In turtles, higher temperatures during egg incubation lead to the production of more females, but in crocodiles and alligators, increased incubation temperatures lead to more male hatchlings being produced. Given the various environmental warming predictions that have been generated, this could lead to problems for these taxa in the future. Both turtles and crocodilians would likely show numerical responses to any warming scenario, but I think that crocodilians would be the taxa which become oocyte-limited due to the increase in production of male hatchlings.
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A fundamental question to evolution theory is: why is sex so common? Specifically, in multicellular animals it is common to have equal sex ratios of females and males. Imagine in such a population a female that asexually produces only female offspring that again produce only female offspring: compared to the rest of the population they would have a doubled fitness (cf. Red-Queen-Hypothesis).
Thus one would ask: why is sex so common? But is it? Humans have about 10^14 cells, which requires at least 46 mitotic cell cycles to grow. Thus, when considering a multicellular animal as a colony of cells, sexual reproduction is still quite rare, in our case only once in at least 46 generations! I wouldn't call that often.
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Dear Marc de Lussanet:
If you think that “the concept of biological species is irrelevant here”, you are far from understanding the situation. Emergence of sexual reproduction and transition to biological species are just the same evolutionary event. Essence of the sexual reproduction is formation of progeny genome by tailoring it from two parental genomes, not by simple copying. The tailoring includes two steps: meiosis is cutting out, fertilization is sewing together. Individual genomes are not reproduced. Gene pool is reproduced instead. It is a qualitative evolutionary leap. Speaking in short, the matter is that large genomes cannot be reproduced with adequate accuracy. Your question was “is sex common?” My answer is yes, if you mean eukaryotes. I would even say that it is obligatory. There are some rare exceptions among most simple eukaryotes that need special explanation. And the relevant people persistently search (and find) such special explanations. Some species retain ability to reproduce asexually yet they obligatory include sexual reproduction in their life cycle. All prokaryotes are asexual. Numerically, they exceed far the eukaryotes, so you may say that sex is not common. I would like to state that sex and sexual reproduction ontologically quite different things. Prokaryotes have sex but not sexual reproduction. Biological meaning of the various genetic exchanges between asexual organisms is quite different from that of sexual reproduction.
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How many rats do I need to determine the sex ratio? How many animals should I use to ensure that the result is statistically significant?
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It depends upon the amount of error you are willing to accept in your estimate of sex ratio. The wikipedia page for "sample size determination" gives a formula for your situation, or search google books for the same. Doing it this way, with an a priori minimum sample size, you're going to need to sex a few hundred individuals to get a good estimate.
Alternatively you could just keep sampling more individuals until the parameter estimate stabilizes.That approach was used in this paper for example: http://dx.doi.org/10.1017/S0031182000061412