Serologic Tests - Science topic

Hi,

We are collecting blood from COVID-19 positive patients for future serology testing.

What would be the best method to preserve the antibodies?

What tube?

Any additional procedures before freezing?

Is negative 20 sufficient or negative 80 required?

Thanks!

why The most definitive way to diagnose a covid is 19 molecular tests and tests

Serology does not have the certainty necessary for diagnosis?

Serology tests are not recommended for identification of COVID-19 infections?

It takes time for the body’s immune system to generate antibodies against a specific pathogen, antibody tests may be negative early in the course of infection; also antibody tests sometimes have trouble distinguishing between infections from closely related pathogens.

Hi All,

I am working on the development of an indirect ELISA in which a certain antigen is coated to the plate in order to detect serum antibodies against that antigen. I am in the process of optimizing the concentration of the antigen coating and the detection antibody concentration using a chessboard approach. I would like to select these concentrations in such a way that a plateau is reached of about 2.0 (OD). Currently I have performed two experiments in which I have titrated both the coating antigen as well as the detection antibody, I also included a titration a higly positive and negative serum as well. The problem I now have is that at low serum dilutions of my positive serum (1/10; 1/50; 1/250) the OD signal is higher than the machine is able to measure (>3; signal overflow), even at 1/80.000 dilution of my detection antibody and a coating concentration of 0.125 ug/ml. Thus, I am not able to reach a plateau within the measuring range of the machine. I am wondering what I could do to reduce the OD signal so that it comes within the dynamic range of the machine. I could try to dilute coating and/or antibody even more, but wouldn't I then risk to lose sensitivity with serum samples that are less positive compared to the high antibody titer serum sample I am using now for optimization purposes? Or might it help to change to a less sensitive TMB substrate? Or reduce the TMB incubation time?

Any advice is much appreciated. Thanks!

I am searching for a good source to read about lateral flow immunoassay manufacturing, which source do you recommend?

The pregnant patient has Behcet's disease and also Rubella IgM positivity.  False positive IgM results may appear because of B cell activation. Can Behcet's disease cause such a response?

I'm planning to design a diagnostic tool for detecting the said parasite. If you have any, please send journals on Paragonimiasis. 

I have collect some samples for rice and I am planning to do the serological test ELISA to detect rice dwarf virus (RDV). The problem I have not found the ELISA kit specified for this virus. So, if anyone could tell me from where I can find it, I will be grateful.

Is vortexing safe to disrupt bacteria in suspensions for antigen preparations?

How important are biobank facilities for your particular research? If you request biomaterial: do you have to pay any access fees?