Seedling - Science topic

I need help with a process and protocol to measure chlorophyll content in Arabidopsis seedlings grown in (-N) media. I also need guidance on how to perform the calculation.

Thinking of nursing Mansonia altisima seedlings

I am looking for some suggestions on appllying PAW to the vegetable seedlings for 2 weeks and compare the results with DI water?

Hello.

For a research project, I need the following information:

1. What is the most appropriate time of day and season to measure the light intensity of the forest floor? Does ambient temperature interfere with recording light intensity?

2. What quadrature size should we use for sampling plants and seedlings?

3. What software do you recommend for statistical analysis of the interaction of vegetation, light intensity, soil factors, litter depth, and soil organic and mineral elements?

Dear colleagues

To do my own research, I need the following two articles. Can any of my colleagues send me these two articles?

Soltani, A., Zeinali, E., Galeshi, S., Latifi, N., 2001. Genetic variation for and interrelationships among seed vigor traits in wheat from the Caspian Sea coast of Iran. Seed Sci. Technol. 29, 653–662.

Soltani, A., Galeshi, S., Zeinali, E., Latifi, N., 2002. Germination, seed reserve utilization and seedling growth of chickpea as affected by salinity and seed size. Seed Sci. Technol. 30, 51–60.

Hello everyone,

I am currently doing my research on herbicide resistance as part of my Ph.D. studies and plan to use the Chenopodium album as my model plant. However, I have encountered significant challenges with its germination.

Here is a summary of the problem:

I would greatly appreciate any advice, protocols, or suggestions from anyone who has experience working with Chenopodium album or other species with challenging germination requirements. Are there specific pre-treatments or conditions that have proven effective for germinating these seeds?

Thank you in advance for your help!

Best regards, Mona

What is the source of energy for plants in the seed and seedling stages, prior to photosynthesis? The seeds have lipid, but also substantial carbohydrate. Do they use carbohydrate during germination? Also, general sources say the fatty acids are converted to glucose (using the glyoxylate cycle) to provide an energy source. But that would also require energy; why not just oxidize the fatty acid? The sequence of utilization must be known. My own area is mammailan metaboism, but I just finished reading "The terroir of whiskey" and became interested in how and when grants utilize their energy sources.

Dear community,

One of our manuscripts has been waiting with the editor for over a year because of the "lack of reviewers." Sadly, the journal editor has repeatedly asked me for reviewer advice. By my heart, I believe in blind peer review, but I'm hopeless at this point. I want to share my manuscript's abstract and ask if anyone is interested in reviewing or advising a reviewer. Thank you in advance!

"Vigorous seedlings guarantee satisfactory production in the forward stages of the vegetation period. This study aimed to evaluate the impact of bio-based rearing water of two mosquito species (Culiseta sp. and Culex sp.) on tomato germination, emergence, and seedling quality. For this purpose, two distinct larval-rearing waters (LRW) (with diverse larval densities), and fry food-applied water (FFW) were used as bio-priming agents. The findings revealed that using bio-based rearing water could enhance the vigor of tomato seeds. When compared to the control group, all Culex sp. derived LRWs had a shorter mean time of germination (MTG). One Culex sp. derived LRW treatment resulted in the shortest MTG (4.35 days), whereas one Culiseta sp. derived LRW treatment resulted in the longest (6.20 days). There were no statistically significant differences in stem length but significant differences in plant length. Plant length was shorter in LRW and FFW than in the control. The stem diameters of plants primed with LRW were generally wider than the control. According to analyses of the plant length, stem length, and stem diameter measurements, the LRW and FFW treatments may have had a reductive influence on plant length but provided significant support for more thick seedlings, which are more beneficial for seedlings. Other germination and growth characteristics (vigor index of germination, emergence percentage, mean time of emergence, vigor index of emergence, plant length, stem length, leaf width, leaf length, stem fresh weight, stem dry weight, root dry weight) did not show significant variation among treatments. The application of LRWs may offer a novel way to improve seedling establishment and tomato growth."

We want to study the growth and development of plant roots under different soil moisture contents. Considering the advantages and disadvantages of several commonly used water control methods, we would like to seek your help to explore a relatively simple and accurate water control method for potted seedlings.

1. Weighing method. A commonly used method is to determine the amount of lost water through weighing and replenish it in a timely manner. But once there are too many biological replicates in our treatment group, it is time-consuming and labor-intensive, and we spend a long time in the weighing and hydration process. On the other hand, as plants grow, their own weight also changes, making the addition of water to each weighing method unscientific.

2. Moisture monitoring instrument. According to the moisture monitoring instrument, the soil moisture content was detected, but according to the several moisture detection instruments we purchased, it was found that there were accuracy issues. At the same time, the measurement value largely depends on the placement of the sensor probe and the influence of the watering position

3. PEG simulation. We would like to know how long PEG can be maintained, i.e. the validity period during treatment. We may consider combining short-term and long-term water control to determine if long-term testing is feasible.

4. After watering, control the water naturally. It can be used for short-term water control, stopping water supply after initial watering, and observing the plant's response during gradual water loss. But we believe that in this situation, there may not be significant differences in the root system, and a long-term water control situation is needed to make the root system different from the control group.

We sincerely invite everyone to help provide a scientifically feasible method for controlling water in potted seedlings. We are extremely grateful for this.

I'm wondering how one handles demographic data that are incomplete to estimate status and trend. For example, consider a 5-stage plant population with stages 1) Seed bank, 2) Seedlings 3) Juvenile 4) Vegetative adult 5) Reproductive adult. For several years one might have yearly counts on all of these, but suppose in recent years, only seed bank data were gathered, or only reproductive adult data were gathered, or both. How does one make use of the full set of incomplete data to estimate status and trend for such a population? I had a plant species in mind, but this is a general question that could pertain to any plant or animal species with incomplete yearly stage, size, or age demographic data.

I am a researcher of Soil science department. 5 months ago I had planted wheat for my research. The research design was split plot design. it has 3 replications, each replication has 2 main plot treatment: Farm yard manure @20t/ha and Biochar @ 20t/ha, and each main plot had 5 treatments:

T1: no N fertilizer,

T2: 100% recommended dose of Prilled urea

T3: 50% recommended dose of Prilled urea

T4: 100% recommended dose of Neem coated urea

T5: 50% recommended dose of Neem Coated Urea

after harvesting of wheat crops, there were wheat crop stubbles left 20 cm above the ground level. The wheat crop residues were not removed and incorporated in the soil after harvesting in April 12. Now in April 20 I had planted Mungbean in the same research trial, and no external fertilizers are used and is grown under residual nutrients of previous planting. The temperature here is 40 degree celcius during sowing of mungbean. I had been thinking to use mungbean as a green manure to increase soil fertility and ground cover in irrigated condition. analysing this prepare suitable research topics that best suits for my research trial.

now after 10 days of sowing there is not enough seedling emergence and i have few seeds remaining that cannot cover all research plots homogenously. i have to take data of biomass of 1m2 from each plot and my re,aining seeds can cover 1m2 area only of each plot out of 12 m2. can i sow seedds to 1 m2 only for the plots that havenot germinated enough seedlings.

I have collected a quarterly data of height and girth for 2 years of the Melia seedlings planted . The data of initial height and Girth of each seedling was also collected. The trial was established in RBD with treatments of VC, FYM and Control. Total 48 seedlings were planted, the trial was divided in 4 blocks and each block contains 12 seedling. (4 seedlings of each treatment). I want to assess the growth performance of seedling under different treatment during course of time. how should I proceed for my analysis . Whether Mixed design ANOVA will be useful here. please suggest

hello everyone, I am new in chip-seq. Recently I want to try arabidopsis thaliana chip-seq by following this protolcol:

I have applied 2 g of A. thaliana seedling to follow the steps describe above, and I used Qsonica Q125 sonicator instead of Sanyo Soniprep 150. However, I failed to shear DNA into <1000bp, most of the genomic DNA has not been broken (first picture), even after 10 minutes of sonication (70% amplitude, 20s/20s on/off, total on time= 10 minutes)

Actually, before I strated to perform this experiment, I tried to use ~7kb plasmid to test the sonication efficiency, and the result was pretty good (second picture).

what should I do? why my chromatin shearing is not working?

I need to get information about the number of chromosomes or DNA content.

I have seedlings from in vitro that were frozen at -20 degrees after liquidation of the culture. They are seedlings from controlled crosses between species.

Classical methods of counting metaphase chromosomes on slides did not work out.

We are considering flow cytometry, although we are concerned that frozen tissue will not be good enough for this.

Do you know any other methods, perhaps more molecular ?

We would appreciate any suggestions for useful protocols.

As part of my PhD project I am using sterile micro-propagated potato plants for experiments.

I do this by cutting nodes from a mother plant, growing in full strength MS media supplemented with 2% sucrose, adjusted to pH 6,4 and 0,8% noble agar.

The plants are then grown in the same media in a closed container with a breathable membrane in the lid.

The plants grow fine, but I get a lot of variation in the size of the new seedlings both within each container but especially between containers. Its seems like for some reason plants in some containers are just growing way faster and some are barely growing.

Anyone has experience with how to get homogeneously sized seedlings?

Thanks in advance!

I have seedlings grown

I want to know how we can avoid the effects of climatic changes during stem cutting in crops. I will cut the mature plants' stems from ground level to get new plantlets, but weather changes may affect the growth of new plantlets. Looking for scientific suggestions.

Dear all,

What are the best methodologies to find out the ectomycorrhizal colonization on roots? I am looking for a publishable methodology to have a tuber sp. percentages of my seedlings.

I need the protocols for tagging tropical mangrove seedings. They need to be numbered and last for at least 10 years (3-4 year censuses).  The only tapes I've been able to find are Dymo tapes and I can control the length and punch a hole for securing it to the seedling.  We were looking for something to secure it to the mangrove seedling like zip ties or something similar.  We also need to purchase over 1000 of them, so price is a key issue.  Any suggestion would be greatly appreciated. If you have experience with long term mangrove seedlings surveys, other suggestions would be welcomed too.

Thanks to all,

Alia.

both the positive and negative effects of shade trees planted along side young cocoa seedlings on the field.

We are working on extraction of apoplast from 2-week-old Arabidopsis seedlings.

We use vacuum infiltration-centrifugation technique.

Unfortunately, we have not been able to isolate apoplast without cytosolic contamination!

When we run SDS-PAGE, Rubisco bands are found!

Do you have any suggestions for improving our apoplast isolation without cytosolic contamination?

Does anyone have any successful experience with apoplast isolation from Arabidopsis?

Thanks

Seedweeds are often a nuisance to sandy beaches, where tourist activities, water sports and swimming are conducted. Tonnes of seaweeds that had failed on the beach are collected and discarded. These biomaterials have immense potential for use in agriculture. Seaweed-based composts are manufactured and used for crop production.

The question articulates around how to use the macroalgae composts, alone or amended by the use of other growing media like perlite, coir etc...

I need to find out days 75% germination for research of my colleague. I have record of germination count of number of wheat seedling every 2 days from 5th to 15 th days. Is there is any formula that I can apply in the excel sheet or formula to calculate the days to 75% germination?

Or any other method to calculate the days to 75% germination?

Hello,

I am designing the experiment on application of Boron (H3BO3) on seedlings and checking if there's any result in blind plants.

Most teams use units kg/ha, only few mg/L.

I would like to convert the data from kg/ha to mg/L, is it possible? Can you please help me?

Hi, we are conducting assays on the ubiquitination modification of a protein expressed in wheat seedling leaves. It is difficult to infiltrate the proteasome inhibitor MG132 into the the thin wheat seedling leaves. We want to know if treatment can be conducted by adding MG132 into the seedling culture solution. Can MG132 be absorbed by roots and transported into leaf tissues?

I have collected several host-specific ecto-mycorrhizal samples from the field and I am willing to inoculate them (Spore suspension) into the same host seedlings to observe growth variation against controlled ones. I am having difficulties comprehending how to inoculate them? I have prepared spore suspension using distilled water dissolving some portion of the fruiting body (crushed). and stored them in refrigerator for later usage. Is my process of preparation good to go or there are more better ways?

Thanks In Advance

I have isolated a fungi species from the trunk of a tree species and I want to test whether this is the causal pathogen that created a canker on that tree stem. I am working on polybag seedlings and is confused about how to inoculate the seedlings.

What are the Methods available for non-destructive biomass estimation of young Miyawaki plantations (young mixed plantations of tree seedlings with DBH less than 5 cm) in tropical condition?

I have grown these sunflower seedling in bubble tray and I want to get suggestions, at how many days after showing, I need to transplant them in the field.

Saudi Arabia has announced plans to plant 10 billion native trees at a rate of one million per week. That's about 50 million trees a year. Is this really possible in the dry desert of Saudi Arabia? has the water requirement of these trees been calculated? In Iran and Khuzestan, which have more suitable conditions, in the warmer months, seedlings need to be irrigated 3 to 4 times a month. Almost every seedling needs about 1 cubic meter (1000 lit) of water in a year. Thus, 50 million trees in a year need 50 million cubic meters of water. If the amount of water needed to produce seedlings in the Nursery is added to this amount of water, a very large volume of water is needed each year. This amount will double for the second year and triple for the third year. Will this volume of water be easily supplied in the dry desert of Saudi Arabia? It seams, this is impossible with such a large scale! . We have to wait for the result. I hope and pray they succeed. for more information you can see the bellow : https://www.al-monitor.com/originals/2021/05/saudi-arabia-plans-plant-10-billion-trees-desert

A Mango growing publication from University of Florida recommends a total of 1.5-3 lb of 8-3-9-2 fertilizer for example. (680-1360 g)

My growing season is about 6-7 months (~26 weeks) and my fertilizer strength is about twice of the mentioned one 14-6-20-4 so let's just assume that I would need about half of the amount which is 340-680g

I prefer to fertilize every time I water a seedling, which is around 10L each week.

When we do the math we get a dose of 13-26g in each watering which is 1.3-2.6g/L.

Should I be worried about that much fertilizer in the irrigation water? (I'm using the most expensive fertilizers with no chloride)

I have very calcareous sandy soil mostly poor in almost everything.

Other than the dead heart?

I am trying to grow rice in a hydroponics system containing 5 % Hoagland's Basal 2 medium (Himedia). My seedlings are turning yellow after a few days of transfer to the nutrient medium. What could be the possible reason for this and how it can be resolved?

I am searching for a method that allows to estimate the effect of exposure to PAR on establishment and growth of tree seedlings below a forest canopy, in a context where completely overcast conditions are hard to find.

I want to conduct a shade tolerance - drought tolerance experiment for tree seedlings from seasonally dry tropical forests of northern Western Ghats

I am using hydroponics to study seedling salt-tolerance of rice genotypes but my 15-days old seedlings are showing this symptom. Can anyone please guide me which nutrient deficiency or disease it can be? The symptom is showing in majority of plants grown in both control and stress...

Has anyone else dealt with static electricity on Arabidopsis plates? The static causes the seedlings to root upwards. Passing your finger along the top of the plate causes the upward root to follow your finger and move. Sometimes the entire seedling will lift off of the media and stick to the lid of the plate. Since the seedling is not rooting properly, it makes drug selection difficult.

Media: 1/2MS + 0.6% agar + BASTA

Good day Scholars,

Studies have it that soluble sugars contents are more accumulated in the K+-deficient leaves, compared to the moderate or high K+ medium. Contrary to this, we found reduced soluble content in K+-deficient leaves (seedling stage).

What could be the possible reasons for such a contrary report?

Comments and suggestions are welcomed.

Thanks

Under the microscope, we can see that there are many red cells on the leaves of cotton seedlings. What kind of cells is it? What kind of function does it have?

The permitted range of element concentrations in cowpea seeds using ICPMS can be found in the literature. However, there is no such range in the case of seedlings. Please let me know the range of concentration of major elements.

How many times greater will the elastic modulus of the trunks of mature urban street trees be than that of their seedlings? For example, Acacia confusa. Thank you for your generous help and suggestions.

I have always had trouble using square plates with media kept vertically. The sugar in the media leaks out in a few days even when thoroughly sealed with parafilm. due to which I am unable to study the morphology of seedlings. please suggest alternatives.

In the soil seed bank germination experiment, the plant seedlings died before being identified. How to reduce this phenomenon?

There is too much water in the germination tray in the greenhouse in winter, which affects the germination of seeds and the growth of seedlings. How to control the water well?

Which method will yield effective result? Is it the inoculation of the seeds or seedlings with PGPB?

I need detailed methods on how to inoculate the seeds as well as the seedlings in greenhouse and field trials.

PDFs will be appreciated. Thanks.

I'm conducting a research on the effect of fire on mimetes fimbrifolius plant.. the challenge I have is to get a method to use to count the seedlings.. what constitutes a seedling in this regard ..

I need recommendations for some North American native trees that produce very deep root systems ( probably more than 2 feet deep) during its seedling or young stage of life.

We're trying to conduct a study on the effects of charcoal ash contaminated soil on the plant growth using Chinese cabbage as samples. Since we're in the middle of a pandemic, the conduction of the experiment is restricted to households and no laboratories are available. Any suggestions on what areas we should study that wouldn't require to travel to other places? Thank you.

Some farmers from Jhalawar, Rajasthan, India are facing the problem of wilting/withering of black cumin seedlings in patches (Images are attached). Soil of the region is medium black with around 8 pH.

I need to give salt stress treatment to Abelmoschus esculentus (okra).

The seeds are grown in soil and I am planning to subject the seedling to salt stress at two weeks old.

I am to stress before vegetative stage.

The treatment will be given using Nacl solution in concentration of 25mM, 50mM and 75mM.

Is it okay to commence the treatment on a two week old seedlings?

Will the Nacl solution be added as irrigation water or a foliar application?

How many days interval should I give for the treatment?

The treatment is expected to last for a period of two weeks.

I really need a guide and recommendation on how to go about the experiment.

Thanks in advance.

Regards,

Adedoyin

I want to screen fenugreek germplasm for early seedling growth, particularly biomass. If biomass of seedling, during early stages like 7-21 days, is associated with seed weight or size, then I can avoid evaluating certain lines based on test weight or size of seed. Or these traits unrelated. Pulse crops may be useful reference.

Dear all, I have a limited supply of Ginkgo biloba seeds from Japan. My purpose is to germinate the seeds and hopefully establish some G. biloba specimens in Costa Rica. What could be the best treatment to trigger germination and subsequently favor the seedling establishment and survival? I have access to these elevations for this purpose: 600, 1200, and 2300 masl. I have been told that the highlands (> 2000 masl) might be best. If you know a few tricks of the trade that you could share, that will save me a lot of time and effort, and some seeds too. The tropics could be challenging for temperate gymnosperms.

I'm trying to isolate plant-growth promoting rhizobacteria (PGPR) that can promote growth in two conifer seedlings (Pinus ponderosa and Pseudotsuga menziesii). Like most PGPR isolation research, I'm screening bacterial colonies for their ability to produce plant hormones (indole-3-acetic acid-IAA, gibberellic acid-GA, and cytokinin-CK). Once I quantify the concentration of IAA, GA, and CK produced, how am I supposed to determine which isolate has the best potential to promote growth? I know high levels of IAA can have inhibitory effects, and too low of concentrations will have no effect. What concentrations of IAA, GA, and CK are physiologically relevant to conifer species and have been found to promote seedling growth? I can't seem to find a clear answer through literature review.

I am interested in identifying seedlings in the forest floor but I am currently concerned about the difficulty of the taxonomy in this life stage of plants. Is there any ID key or manual ? Is it hard or easy ? Thank you!

In our recent work we realized that In root outer cell layers there were Fulvic acid compounds bound to heavy metals. It’s really weird to see such a compound in root tissues of rice seedlings growing under hydroponic culture! I hope if any expert could explain this result. Everyone knows that plant systems are unable to form Fulvic acid or at least no one has yet observed or claimed this in the literature. Our XANES result showed that this compound doesn’t carry any carboxylic group and is very much similar to FA structure. It would be appreciated if anyone of you could explain this finding.

UV-C water treatments

Okra seedlings were raised.

Pathogen inoculation was done during sowing.

This was followed by biological control.

Dickson quality index (DQI) = total dry mass / (Shoot:Root ratio + SQ)

Where, Sturdiness quotient (sq) = plant height / root collar diameter

How effective is it to calculate DQI when pathogen inoculation was done while seed-sowing, followed by biological control (Trichoderma/ Pseudomonas/ Botanicals application)?

Thanks!

The formula for the Seedling vigour index is given as:

Kumar, B., Verma, S. K., Ram, G., & Singh, H. P. (2012). Temperature relations for seed germination potential and seedling vigor in Palmarosa (Cymbopogon martinii). Journal of Crop Improvement, 26(6), 791-801.

But, we, in our research did the thinning out on the 7th day and raised only one model plant per replication (of particular treatment) for the next 9 days i.e. 16 DAS. We did destructive sampling of the model plants on the 16th day. So we don't have the length and weight data of the sample plants on 7th day but only on the 16th day.

Shall we proceed to calculate the Seedling vigour index of thinned out seedlings only (on day 7th)? Or combine both data directly (of 7th and 16th day)?

Hello.

I'm working with germination of various species of a genus, while analizyng the data I have found a correlation:

The bigger the seed of a species, the range of time the seeds germinate is wider.

The smaller the seed of a species, the range of time the seeds germinate is shorter.

Trying to explain this, i have a tought... This could be 'cause a bigger seed have more chances to resist dormancy and so, so they can have the "option" to germinate in diferent times, so the seedlings don;t compete between each other.

Does this make sense? Any one have a peper or some reference that back up this tought?

I'll love to hear your toughts and your help with the literature.

I have had some success germinating Ginkgo seeds in Costa Rica. I would like to know recommendations to secure seedling establishment. Right now they are in 1.5 L plastic bags on organic soil at field capacity. Do they need a fertilization supplement? What could be the best tips to share to secure seedling growth and establishment? How long could they take to reach a size amenable for transplantation into the field?

We generally use 50 X 50 m or 100 X 100 m quadrats to calculate the basic ecological parameters within a hectare of land. Within 50 X 50 m or 100 X 100 m quadrats, we used to laid 10 X 10 m quadrats for sampling tree species, 5 X 5 m for shrubs & seedlings, and 1x 1 m for herbs.

I think to improve the growth of pomegranate seedlings when they planted in the orchard soil because some of seedlings may be died or growth weakly. The rooting stimulants were applied to get out more roots of cuttings, therefore I hope the ability of these stimulants benefit the transplants when were planting.

I have few young mango seedlings that I had moved to the ground way too early. My soil is calcareous and very alkaline. I have been trying several combinations of fertilizers but I apparently made things worse.

This time I'm interested in finding out the deficiencies that cause new buds to cease growing and freeze. Giving a balanced fertilizer (18-18-18 with chelated micros didn't help).

Looking at the nutrients responsible for cell generation/elongation made me focus on Boron/Calcium/P maybe/Zn?

Could it be that some seedlings just won't respond well in very alkaline soils?

The soil as the test shows is mostly calcium but I would take that with a grain of salt, as multiple fertilizer applications/irrigations and the layers of vegetable compost must have changed a lot since then.

Can you think of anything else please? More precisely the nutrients that are crucial for cell generation and elongation. Also the ones that are more likely to suffer when overfeeding NPK.

I find this one very tricky as it seems to be an extra silent deficiency where symptoms are hardly visible.

Let me know your thoughts please. Thanks

Here is my soil test. As you can see Calcium is an issue and pH is quite high.

Let's not focus on micronutrients for now please. I'm trying to help very young mango seedlings get established, and I'm worried about P fixation.

What would you pick as an NPK of choice. Suppose we start from a balanced ratio like 18-18-18 for example, would you rather prefer more N, more P or more K or any other combinations of those?

Again, the goal is to help very young seedlings get established, they're actually up to 1 year old from planting the seed.

Thanks

Hello,

Does anyone have any advice on how to measure soil moisture or hydraulic conductivity in a microbial greenhouse experiment? Seedlings will be grown in D40 cones and half of the sample will be given a drought treatment using drip emitters.

What is the most efficient and reliable way to measure soil moisture or hydraulic conductance in dry soil in a greenhouse setting?

Thanks.

Dear Researchers!

The attached images are stably expressing GFP fused target proteins in Brassica rapa young seedlings (Note: leaves were treated with Clearsee solution prior to imaging, to remove autofluorescence). I have three questions to ask.

1. In figure A, what are those strong signalled round shapes (different in numbers) found inside the cells?

2. In figure B, Is it ok to have GFP signals in stomata pores or those are artefacts?

3. In figure C, what are those fluorescence holes on the cell surface?

Can anyone help me in this regard?

Thanks in advance!

Hi. I'm growing Arabidopsis thaliana on ágar medium (0.8%), aiming to extract some enzymes of the seedlings 120 hours after germination (just 5 days). Do I need to prepare the MS medium? Or can I just grow the seeds in the agar medium without the MS medium?

Dear colleagues,

300 accessions of Dolichos lablab have been screened for drought tolerance in the green-house based on Augmented block design.

In this screening project, two consecutive experiments were conducted to involve 10 weeks each. In each experiment, the project involved drought inducing period by withholding water as well as water supplying period to induce plant recovery.

Both quantitative and qualitative data were collected during germination and seedling stage of the plants. The data included chlorophyll content, height of the plants, number of leaves, wilting%, overall wilting scales, recovery%, shifting and folding of leaves etc.

I would like therefore to request for your advice on the best approach on data analysis so that I can determine the best drought tolerant genotypes.

When transplanting tropical seedlings, it's very common for the taproot to get damaged. I was wondering if there is any information on the effect of the taproot on the resistance of seedlings, and whether the plant would be able to regrow a replacement root that goes deep in the soil. Thanks

Hi! It’s been weeks since I try to figure out how to manage missing value in SPSS and R. what I have read is that there are types of missing value and few approaches to manage the missing value. But I was unable to find the right answers.

My experiment is, there are two factors which are spacing (plot) and fertilizer (subplot) treatment for Terminalia seedlings. The experiment has three replications, each subplot has nine seedlings. The experimental design is RCBD. Unfortunately, there are some seedlings that are died in the experiment.

So, my questions are the dead seedlings is count as missing value? Do I have to exclude the dead seedlings data from the dataset for further analysis such as normality test, ANOVA, Post Hoc and Regression?

I have read that in SPSS there are options for missing values but I just wondering is that the correct method to manage the dead seedlings data. Are there any references to manage data of dead seedlings in the experiment? I try to find it but had no luck.

Please help me.

Dear colleagues, fellow scientists and students,

I am working on establishing of protocol for BioID in plants. I am aware of already existing protocols such as cell culture, the transient transformation of tobaccos and root culture. For our purposes, we will need to work with seedlings and I was wondering if someone ever tried to perhaps transfer the seedling onto solid MS media enhanced with biotin. Could that work? What would be a good concentration to start with? I'd be grateful for any insights or links to relevant literature backing up the yes-no argument.

Thank you and all the best in your research!

We have sufficient seeds of 120 rice genotypes. We want to evaluate the genetic parameters related to early seedling vigour through replicated trials of these 120 genotypes. We didn't want to use Augmented design. Can anyone suggest us the appropriate experimental design for the same? I shall be highly grateful.

Most of the farmers are broadcasting Urea at the time of puddling. Is this is the best time to apply urea just before transplantion? Actually, urea is not persists for longer time, roots are also not well developed in this stage, and seedlings are not also properly established in the soil to uptake nitrogen from urea. So, could please suggest me which is the best time to apply urea, at puddling time or after 1 week of transplantation ???

The Arabidopsis wild type plants transformed with the transporter gene construct (gene + PBI121) has given me the T0 seeds. The T0 seedlings selected with kanamycin (50 mg/L) when placed in soil, grow up to the silique stage after which the plants are getting wilted and eventually die. Anyone could help me with this?

I am working on the micropropagation of Tea, I need to speed up the growth of my seedlings in a short period of time. I need to know which kind of hormones I should apply in my growth medium. I was very thankful you are considering my problem.

I am performing research on tomato seedling for which I need to measure the seedling height ( root length + shoot length ) and diameter. Is there any procedure we should follow to measure the root length of seedling and also from which point should we measure the seedling diameter?