Science topics: Biological ScienceBotanySeedling
Seedling - Science topic
Very young plant after GERMINATION of SEEDS.
Questions related to Seedling
I need the protocols for tagging tropical mangrove seedings. They need to be numbered and last for at least 10 years (3-4 year censuses). The only tapes I've been able to find are Dymo tapes and I can control the length and punch a hole for securing it to the seedling. We were looking for something to secure it to the mangrove seedling like zip ties or something similar. We also need to purchase over 1000 of them, so price is a key issue. Any suggestion would be greatly appreciated. If you have experience with long term mangrove seedlings surveys, other suggestions would be welcomed too.
Thanks to all,
both the positive and negative effects of shade trees planted along side young cocoa seedlings on the field.
We are working on extraction of apoplast from 2-week-old Arabidopsis seedlings.
We use vacuum infiltration-centrifugation technique.
Unfortunately, we have not been able to isolate apoplast without cytosolic contamination!
When we run SDS-PAGE, Rubisco bands are found!
Do you have any suggestions for improving our apoplast isolation without cytosolic contamination?
Does anyone have any successful experience with apoplast isolation from Arabidopsis?
Seedweeds are often a nuisance to sandy beaches, where tourist activities, water sports and swimming are conducted. Tonnes of seaweeds that had failed on the beach are collected and discarded. These biomaterials have immense potential for use in agriculture. Seaweed-based composts are manufactured and used for crop production.
The question articulates around how to use the macroalgae composts, alone or amended by the use of other growing media like perlite, coir etc...
I need to find out days 75% germination for research of my colleague. I have record of germination count of number of wheat seedling every 2 days from 5th to 15 th days. Is there is any formula that I can apply in the excel sheet or formula to calculate the days to 75% germination?
Or any other method to calculate the days to 75% germination?
I am designing the experiment on application of Boron (H3BO3) on seedlings and checking if there's any result in blind plants.
Most teams use units kg/ha, only few mg/L.
I would like to convert the data from kg/ha to mg/L, is it possible? Can you please help me?
Hi, we are conducting assays on the ubiquitination modification of a protein expressed in wheat seedling leaves. It is difficult to infiltrate the proteasome inhibitor MG132 into the the thin wheat seedling leaves. We want to know if treatment can be conducted by adding MG132 into the seedling culture solution. Can MG132 be absorbed by roots and transported into leaf tissues?
I have collected several host-specific ecto-mycorrhizal samples from the field and I am willing to inoculate them (Spore suspension) into the same host seedlings to observe growth variation against controlled ones. I am having difficulties comprehending how to inoculate them? I have prepared spore suspension using distilled water dissolving some portion of the fruiting body (crushed). and stored them in refrigerator for later usage. Is my process of preparation good to go or there are more better ways?
Thanks In Advance
I have isolated a fungi species from the trunk of a tree species and I want to test whether this is the causal pathogen that created a canker on that tree stem. I am working on polybag seedlings and is confused about how to inoculate the seedlings.
What are the Methods available for non-destructive biomass estimation of young Miyawaki plantations (young mixed plantations of tree seedlings with DBH less than 5 cm) in tropical condition?
I have grown these sunflower seedling in bubble tray and I want to get suggestions, at how many days after showing, I need to transplant them in the field.
Saudi Arabia has announced plans to plant 10 billion native trees at a rate of one million per week. That's about 50 million trees a year. Is this really possible in the dry desert of Saudi Arabia? has the water requirement of these trees been calculated? In Iran and Khuzestan, which have more suitable conditions, in the warmer months, seedlings need to be irrigated 3 to 4 times a month. Almost every seedling needs about 1 cubic meter (1000 lit) of water in a year. Thus, 50 million trees in a year need 50 million cubic meters of water. If the amount of water needed to produce seedlings in the Nursery is added to this amount of water, a very large volume of water is needed each year. This amount will double for the second year and triple for the third year. Will this volume of water be easily supplied in the dry desert of Saudi Arabia? It seams, this is impossible with such a large scale! . We have to wait for the result. I hope and pray they succeed. for more information you can see the bellow : https://www.al-monitor.com/originals/2021/05/saudi-arabia-plans-plant-10-billion-trees-desert
A Mango growing publication from University of Florida recommends a total of 1.5-3 lb of 8-3-9-2 fertilizer for example. (680-1360 g)
My growing season is about 6-7 months (~26 weeks) and my fertilizer strength is about twice of the mentioned one 14-6-20-4 so let's just assume that I would need about half of the amount which is 340-680g
I prefer to fertilize every time I water a seedling, which is around 10L each week.
When we do the math we get a dose of 13-26g in each watering which is 1.3-2.6g/L.
Should I be worried about that much fertilizer in the irrigation water? (I'm using the most expensive fertilizers with no chloride)
I have very calcareous sandy soil mostly poor in almost everything.
I am trying to grow rice in a hydroponics system containing 5 % Hoagland's Basal 2 medium (Himedia). My seedlings are turning yellow after a few days of transfer to the nutrient medium. What could be the possible reason for this and how it can be resolved?
I am searching for a method that allows to estimate the effect of exposure to PAR on establishment and growth of tree seedlings below a forest canopy, in a context where completely overcast conditions are hard to find.
I want to conduct a shade tolerance - drought tolerance experiment for tree seedlings from seasonally dry tropical forests of northern Western Ghats
I am using hydroponics to study seedling salt-tolerance of rice genotypes but my 15-days old seedlings are showing this symptom. Can anyone please guide me which nutrient deficiency or disease it can be? The symptom is showing in majority of plants grown in both control and stress...
Im having an issue while transferring germinated seeds to MS/2 medium + phytagel.
Seeds were germinated in H2Od, these (3d old) were sterilized with 0.05% NaClO before transfer to nutritive medium (described above)... and having problems with contamination. Should I try higher concentration of NaClO to sterilize germinated seeds or use anothery type of sterilizing solution? (want to avoid damage to seedling while avoiding contamination)
(seeds were immersed in 70% ethanol and 2% NaClO prior to germination)
Has anyone else dealt with static electricity on Arabidopsis plates? The static causes the seedlings to root upwards. Passing your finger along the top of the plate causes the upward root to follow your finger and move. Sometimes the entire seedling will lift off of the media and stick to the lid of the plate. Since the seedling is not rooting properly, it makes drug selection difficult.
Media: 1/2MS + 0.6% agar + BASTA
Good day Scholars,
Studies have it that soluble sugars contents are more accumulated in the K+-deficient leaves, compared to the moderate or high K+ medium. Contrary to this, we found reduced soluble content in K+-deficient leaves (seedling stage).
What could be the possible reasons for such a contrary report?
Comments and suggestions are welcomed.
Under the microscope, we can see that there are many red cells on the leaves of cotton seedlings. What kind of cells is it? What kind of function does it have?
The permitted range of element concentrations in cowpea seeds using ICPMS can be found in the literature. However, there is no such range in the case of seedlings. Please let me know the range of concentration of major elements.
Effect of indole acetic acid (IAA) on the germination and seedling growth of crop plants
How many times greater will the elastic modulus of the trunks of mature urban street trees be than that of their seedlings? For example, Acacia confusa. Thank you for your generous help and suggestions.
I have always had trouble using square plates with media kept vertically. The sugar in the media leaks out in a few days even when thoroughly sealed with parafilm. due to which I am unable to study the morphology of seedlings. please suggest alternatives.
We generally use 50 X 50 m or 100 X 100 m quadrats to calculate the basic ecological parameters within a hectare of land. Within 50 X 50 m or 100 X 100 m quadrats, we used to laid 10 X 10 m quadrats for sampling tree species, 5 X 5 m for shrubs & seedlings, and 1x 1 m for herbs.
In the soil seed bank germination experiment, the plant seedlings died before being identified. How to reduce this phenomenon?
There is too much water in the germination tray in the greenhouse in winter, which affects the germination of seeds and the growth of seedlings. How to control the water well?
Which method will yield effective result? Is it the inoculation of the seeds or seedlings with PGPB?
I need detailed methods on how to inoculate the seeds as well as the seedlings in greenhouse and field trials.
PDFs will be appreciated. Thanks.
I'm particularly interested in identification of temperate forest plant species. Thanks!
I'm conducting a research on the effect of fire on mimetes fimbrifolius plant.. the challenge I have is to get a method to use to count the seedlings.. what constitutes a seedling in this regard ..
I need recommendations for some North American native trees that produce very deep root systems ( probably more than 2 feet deep) during its seedling or young stage of life.
We're trying to conduct a study on the effects of charcoal ash contaminated soil on the plant growth using Chinese cabbage as samples. Since we're in the middle of a pandemic, the conduction of the experiment is restricted to households and no laboratories are available. Any suggestions on what areas we should study that wouldn't require to travel to other places? Thank you.
Some farmers from Jhalawar, Rajasthan, India are facing the problem of wilting/withering of black cumin seedlings in patches (Images are attached). Soil of the region is medium black with around 8 pH.
I need to give salt stress treatment to Abelmoschus esculentus (okra).
The seeds are grown in soil and I am planning to subject the seedling to salt stress at two weeks old.
I am to stress before vegetative stage.
The treatment will be given using Nacl solution in concentration of 25mM, 50mM and 75mM.
Is it okay to commence the treatment on a two week old seedlings?
Will the Nacl solution be added as irrigation water or a foliar application?
How many days interval should I give for the treatment?
The treatment is expected to last for a period of two weeks.
I really need a guide and recommendation on how to go about the experiment.
Thanks in advance.
I want to screen fenugreek germplasm for early seedling growth, particularly biomass. If biomass of seedling, during early stages like 7-21 days, is associated with seed weight or size, then I can avoid evaluating certain lines based on test weight or size of seed. Or these traits unrelated. Pulse crops may be useful reference.
Dear all, I have a limited supply of Ginkgo biloba seeds from Japan. My purpose is to germinate the seeds and hopefully establish some G. biloba specimens in Costa Rica. What could be the best treatment to trigger germination and subsequently favor the seedling establishment and survival? I have access to these elevations for this purpose: 600, 1200, and 2300 masl. I have been told that the highlands (> 2000 masl) might be best. If you know a few tricks of the trade that you could share, that will save me a lot of time and effort, and some seeds too. The tropics could be challenging for temperate gymnosperms.
I'm trying to isolate plant-growth promoting rhizobacteria (PGPR) that can promote growth in two conifer seedlings (Pinus ponderosa and Pseudotsuga menziesii). Like most PGPR isolation research, I'm screening bacterial colonies for their ability to produce plant hormones (indole-3-acetic acid-IAA, gibberellic acid-GA, and cytokinin-CK). Once I quantify the concentration of IAA, GA, and CK produced, how am I supposed to determine which isolate has the best potential to promote growth? I know high levels of IAA can have inhibitory effects, and too low of concentrations will have no effect. What concentrations of IAA, GA, and CK are physiologically relevant to conifer species and have been found to promote seedling growth? I can't seem to find a clear answer through literature review.
I am interested in identifying seedlings in the forest floor but I am currently concerned about the difficulty of the taxonomy in this life stage of plants. Is there any ID key or manual ? Is it hard or easy ? Thank you!
In our recent work we realized that In root outer cell layers there were Fulvic acid compounds bound to heavy metals. It’s really weird to see such a compound in root tissues of rice seedlings growing under hydroponic culture! I hope if any expert could explain this result. Everyone knows that plant systems are unable to form Fulvic acid or at least no one has yet observed or claimed this in the literature. Our XANES result showed that this compound doesn’t carry any carboxylic group and is very much similar to FA structure. It would be appreciated if anyone of you could explain this finding.
Okra seedlings were raised.
Pathogen inoculation was done during sowing.
This was followed by biological control.
Dickson quality index (DQI) = total dry mass / (Shoot:Root ratio + SQ)
Where, Sturdiness quotient (sq) = plant height / root collar diameter
How effective is it to calculate DQI when pathogen inoculation was done while seed-sowing, followed by biological control (Trichoderma/ Pseudomonas/ Botanicals application)?
The formula for the Seedling vigour index is given as:
Seedling vigour index I = Germination (%) × mean seedling length
Seedling vigour index II = Germination (%) × mean seedling dry mass
In the paper quoted below, The seedling length was taken on the 7th day.
Kumar, B., Verma, S. K., Ram, G., & Singh, H. P. (2012). Temperature relations for seed germination potential and seedling vigor in Palmarosa (Cymbopogon martinii). Journal of Crop Improvement, 26(6), 791-801.
But, we, in our research did the thinning out on the 7th day and raised only one model plant per replication (of particular treatment) for the next 9 days i.e. 16 DAS. We did destructive sampling of the model plants on the 16th day. So we don't have the length and weight data of the sample plants on 7th day but only on the 16th day.
Shall we proceed to calculate the Seedling vigour index of thinned out seedlings only (on day 7th)? Or combine both data directly (of 7th and 16th day)?
I'm working with germination of various species of a genus, while analizyng the data I have found a correlation:
The bigger the seed of a species, the range of time the seeds germinate is wider.
The smaller the seed of a species, the range of time the seeds germinate is shorter.
Trying to explain this, i have a tought... This could be 'cause a bigger seed have more chances to resist dormancy and so, so they can have the "option" to germinate in diferent times, so the seedlings don;t compete between each other.
Does this make sense? Any one have a peper or some reference that back up this tought?
I'll love to hear your toughts and your help with the literature.
I have had some success germinating Ginkgo seeds in Costa Rica. I would like to know recommendations to secure seedling establishment. Right now they are in 1.5 L plastic bags on organic soil at field capacity. Do they need a fertilization supplement? What could be the best tips to share to secure seedling growth and establishment? How long could they take to reach a size amenable for transplantation into the field?
I think to improve the growth of pomegranate seedlings when they planted in the orchard soil because some of seedlings may be died or growth weakly. The rooting stimulants were applied to get out more roots of cuttings, therefore I hope the ability of these stimulants benefit the transplants when were planting.
I have few young mango seedlings that I had moved to the ground way too early. My soil is calcareous and very alkaline. I have been trying several combinations of fertilizers but I apparently made things worse.
This time I'm interested in finding out the deficiencies that cause new buds to cease growing and freeze. Giving a balanced fertilizer (18-18-18 with chelated micros didn't help).
Looking at the nutrients responsible for cell generation/elongation made me focus on Boron/Calcium/P maybe/Zn?
Could it be that some seedlings just won't respond well in very alkaline soils?
The soil as the test shows is mostly calcium but I would take that with a grain of salt, as multiple fertilizer applications/irrigations and the layers of vegetable compost must have changed a lot since then.
Can you think of anything else please? More precisely the nutrients that are crucial for cell generation and elongation. Also the ones that are more likely to suffer when overfeeding NPK.
I find this one very tricky as it seems to be an extra silent deficiency where symptoms are hardly visible.
Let me know your thoughts please. Thanks
Here is my soil test. As you can see Calcium is an issue and pH is quite high.
Let's not focus on micronutrients for now please. I'm trying to help very young mango seedlings get established, and I'm worried about P fixation.
What would you pick as an NPK of choice. Suppose we start from a balanced ratio like 18-18-18 for example, would you rather prefer more N, more P or more K or any other combinations of those?
Again, the goal is to help very young seedlings get established, they're actually up to 1 year old from planting the seed.
Does anyone have any advice on how to measure soil moisture or hydraulic conductivity in a microbial greenhouse experiment? Seedlings will be grown in D40 cones and half of the sample will be given a drought treatment using drip emitters.
What is the most efficient and reliable way to measure soil moisture or hydraulic conductance in dry soil in a greenhouse setting?
Hi. I'm growing Arabidopsis thaliana on ágar medium (0.8%), aiming to extract some enzymes of the seedlings 120 hours after germination (just 5 days). Do I need to prepare the MS medium? Or can I just grow the seeds in the agar medium without the MS medium?
I used Agrobacterium tumefaciens to transform Arabidopsis thaliana, and the vectors were constructed on pCAMBIA1301 and pCAMBIA1302 with a hygromycin resistance gene on them.
I have harvested T0 seeds, and planted them on a selective medium (MS agar + 50mg/L hygromycin B), but after more than a week, all the seedlings looked the same as the negative control (col-0). (Attach pictures)
Even though I repeated the transformation experiment and harvested the seeds (To), used freshly prepared MS medium, and used freshly prepared hygromycin (bought a new one),
So even the second time the results were the same as the first time, all the seedlings looked the same as the negative control (col-0).
Has anyone encountered this kind of problem?
What should I do when I choose existing transformed seedlings?
Thank you very much.
300 accessions of Dolichos lablab have been screened for drought tolerance in the green-house based on Augmented block design.
In this screening project, two consecutive experiments were conducted to involve 10 weeks each. In each experiment, the project involved drought inducing period by withholding water as well as water supplying period to induce plant recovery.
Both quantitative and qualitative data were collected during germination and seedling stage of the plants. The data included chlorophyll content, height of the plants, number of leaves, wilting%, overall wilting scales, recovery%, shifting and folding of leaves etc.
I would like therefore to request for your advice on the best approach on data analysis so that I can determine the best drought tolerant genotypes.
The attached images are stably expressing GFP fused target proteins in Brassica rapa young seedlings (Note: leaves were treated with Clearsee solution prior to imaging, to remove autofluorescence). I have three questions to ask.
1. In figure A, what are those strong signalled round shapes (different in numbers) found inside the cells?
2. In figure B, Is it ok to have GFP signals in stomata pores or those are artefacts?
3. In figure C, what are those fluorescence holes on the cell surface?
Can anyone help me in this regard?
Thanks in advance!
When transplanting tropical seedlings, it's very common for the taproot to get damaged. I was wondering if there is any information on the effect of the taproot on the resistance of seedlings, and whether the plant would be able to regrow a replacement root that goes deep in the soil. Thanks
Hi! It’s been weeks since I try to figure out how to manage missing value in SPSS and R. what I have read is that there are types of missing value and few approaches to manage the missing value. But I was unable to find the right answers.
My experiment is, there are two factors which are spacing (plot) and fertilizer (subplot) treatment for Terminalia seedlings. The experiment has three replications, each subplot has nine seedlings. The experimental design is RCBD. Unfortunately, there are some seedlings that are died in the experiment.
So, my questions are the dead seedlings is count as missing value? Do I have to exclude the dead seedlings data from the dataset for further analysis such as normality test, ANOVA, Post Hoc and Regression?
I have read that in SPSS there are options for missing values but I just wondering is that the correct method to manage the dead seedlings data. Are there any references to manage data of dead seedlings in the experiment? I try to find it but had no luck.
Please help me.
Dear colleagues, fellow scientists and students,
I am working on establishing of protocol for BioID in plants. I am aware of already existing protocols such as cell culture, the transient transformation of tobaccos and root culture. For our purposes, we will need to work with seedlings and I was wondering if someone ever tried to perhaps transfer the seedling onto solid MS media enhanced with biotin. Could that work? What would be a good concentration to start with? I'd be grateful for any insights or links to relevant literature backing up the yes-no argument.
Thank you and all the best in your research!
We have sufficient seeds of 120 rice genotypes. We want to evaluate the genetic parameters related to early seedling vigour through replicated trials of these 120 genotypes. We didn't want to use Augmented design. Can anyone suggest us the appropriate experimental design for the same? I shall be highly grateful.
Most of the farmers are broadcasting Urea at the time of puddling. Is this is the best time to apply urea just before transplantion? Actually, urea is not persists for longer time, roots are also not well developed in this stage, and seedlings are not also properly established in the soil to uptake nitrogen from urea. So, could please suggest me which is the best time to apply urea, at puddling time or after 1 week of transplantation ???
The Arabidopsis wild type plants transformed with the transporter gene construct (gene + PBI121) has given me the T0 seeds. The T0 seedlings selected with kanamycin (50 mg/L) when placed in soil, grow up to the silique stage after which the plants are getting wilted and eventually die. Anyone could help me with this?
I am performing research on tomato seedling for which I need to measure the seedling height ( root length + shoot length ) and diameter. Is there any procedure we should follow to measure the root length of seedling and also from which point should we measure the seedling diameter?
We use the standard method with TRI Reagent and chloroform. The ratio of RNA from leaves is about 1.6-1.7, but from roots and hypocotyl it is only 0.8-0.9. Do you know any efficient system for purifying the isolate from undesirable compounds?
I am working on the micropropagation of Tea, I need to speed up the growth of my seedlings in a short period of time. I need to know which kind of hormones I should apply in my growth medium. I was very thankful you are considering my problem.
I am trying to interpret foliar analyses of sugar maple tree and seedlings using Diagnosis and Recommendation Integrated System (DRIS) approach. To do this you compare observed:optimal ratios of foliar nutrients. I am looking for literature on optimal ratios for sugar maples.
I isolated the pathogen from okra seedling infected by damping off and observed macro conidia under microscope but could not know the species.
Hi I am trying to establish a germination assay for Arabidopsis. I grew Arabidopsis seedling in MS medium and medium with Gibberellic acid (GA) as a positive control
However I didn't see any difference. What do you think I could change?
Medium: Murashige and skoog (4.4 g/l ) Sacarose (10 g/l) Agar (8 g/l)
GA Concentrations: 1 µM and 100µ M
I prepared a stock solution of 10 mM in water and I added to adjust the pH (NaOH 50%)
I sterilized the seedling and placed them in the medium immediately. I checked them after 72 h
Thank you :)
We have to study the root anatomy of almost 480 plants, 80 plants after every 2 months for 1 year. Kindly suggest whether should we plant the seedlings in pot in greenhouse or in experimental plots in natural conditions? Which will give us better results.
I am planning to induce the different osmotic stress using PEG-6000 and plants will be grown in hydroponics till 14-15 days to study the root's parameter.
But while inducing the stress using PEG-6000, do I need to germinate the seeds first and then transfer to the hydroponics ? or Can I directly germinate the seeds under stress from the very beginning (i.e. putting the sterilized seeds on the media)?
I wish to mimic the field conditions for the study since I will use the selected genotypes for field experiments.
I'm doing MDA (malondialdehyde) experiment with Arabidopsis seedlings under different salinity stress and the reaction turns yellow, the absorbances are within the range, but I still have the doubt of the veracity of my data. Is it normal to take this color and not pink or red on plants?
In the early non-bearing years, if we measure the average leaf area (not the LAI!), can we screen the the seedling population for fruit size?
This layer normally maintained 1.5-2.5 cm thick. If it become less than 1.5 cm and around 1.0 cm, whats is happen?
I have an experiment in which the root-tip angles of 6 lines of Arabidopsis seedlings have been measured every 2h from 0-14h.
How do I compare the change in root angle along the time course of the experiment between groups so that I can see which specific lines have a significant difference in root angle and at what time this occurs.
I have tried a repeated-measures two-way ANOVA using SPSS but cannot work out how to see which specific lines differ from one another at each timepoint - any help would be much appreciated!
I have conducted a repeated-measures ANOVA and a Tukey post hoc test. The data I have is for 3 seedling mutant lines, I am comparing their root growth over a timecourse of 19 hours. Within each group, there are 35 seedlings measured at each time point. The Tukey test gives an indication of significance between the seedling lines, however does not show at what time this significance occurs, therefore I have been trying to find this out using a one-way ANOVA at each time point, which doesn't make sense as each seedling is repeatedly measured.
I have been doing work on seedling stomata. the old images cannot be measured in um but can in pixel. so I need to convert the measurement to um.