Seed Germination - Science topic

how much cost

First, you need to make sure all of the seeds are viable. You can use the flotation test for this (viable seeds will sink in water). Then you place the viable seeds (at least 30) on your medium (pot or plate), using at least 6 of these (these are your subsamples). You replicate this whole process at least 3 times, but each replicate must be distant from each other. Environmental conditions must be the same for all replicates and the pots/plates must be rotated within each replicate to ensure equal microclimate conditions. So 30 seeds on each of 6 plates/pots, replicated 3 times = 18 plates/pots :)

Simply work out the cumulative germination percent from daily count data so you can easily find the time interval when it cross 75.

Melkamu Dugassa Erena Auxin-producing bacteria in vermicompost can stimulate primary roots development and it will delay plant emergence. You can measure root length in control and in pots treated with vermicompost

Yes, sterilization of the nanoparticle solution is typically required before adding to Murashige and Skoog (MS) media for seed germination to maintain aseptic conditions. Here's a short protocol:

1. Sterilisation: Filter-sterilize the nanoparticle solution using a syringe filter (typically 0.22 µm pore size) to remove any microbial contaminants.

2. Preparation of Media: Prepare and autoclave the MS media according to standard protocols.

3. Cooling: Allow the autoclaved MS media to cool down to approximately 45-50°C.

4. Addition of Nanoparticles: Add the sterilised nanoparticle solution to the cooled MS media under aseptic conditions, usually in a laminar flow hood.

5. Mixing: Mix gently but thoroughly to ensure even distribution of the nanoparticles in the media.

Ensure that the concentration of nanoparticles and the volume added do not alter the desired pH or osmotic balance of the MS media.

yes

Not sure if there is anything unique about ABA that would preclude this, but this is how my lab does chemical treatments in media:

For the 100x100mm square plates that my lab uses, 50 mL of media makes two plates. So for a 1uM ABA condition, I would add 50uL to 50 mL (using C1V1=C2V2 formula) of media and pour two plates. If you are making lots of plates, your could consider mixing the ABA into a bigger volume of media in a bottle, but this is how we make relatively few plates.

Also many times it is written without any unit means only value is written but yes seed germination, seedling vigour I, and seedling vigour II comes under germination potential of any seed/crop which can be mentioned in percentage. For a better understanding, you can read a few papers and get some idea of how to write the results for SVI and SVII. hereby I have also attached a paper for your kind reference.

Dear Kaushik Shandilya. I sincerely thank you for your recommendations.

@Kaushik Shandilya

Many seeds need light to trigger their germination. The deposition of these seeds at increased depth is likely to favor dormancy over germination.

Leave the mixture in an ultralow temperature freezer for 24 hours. The ethanol will separate the soluble components of the extract during this soaking period. Then apply aquades. Or simply remove the non-soluble elements by filtration since not all plant material will dissolve in ethanol :)

Andrew Paul McKenzie Pegman Hi! thanks for replying. I earlier read about mucilage and as I understand mucilage is the collective term used for the secretion by seed during germination. I am looking for specific term for these cells in image. These are single free floating round cells and these are viable even when they are away from the seed coat.

Rivera JD. et al 2022. Determination of gibberellic acid and abscisic acid in (Zea mays L.) (ICA-V305) seeds germinated using dynamic sonication assisted solvent extraction and maceration. MethodsX, 9.

https://doi.org/10.1016/j.mex.2022.101821.

Dear Pablo Reguera

If you are having issues with fungal contamination you maintain the aseptic condition. Sterilize everything that is being used in the experiment, like filter paper, Petri plates, and distilled water, and carry out your experiment in a laminar. Sterilization of seeds with a 30-45 seconds dip in 1% NaOCl and 3 successive 30 seconds of wash on sterilized distilled water is a must to prevent any contamination.

Kind regards

Jiwan

Cleber Juliano Neves Chaves I have not found in literature any direct comparison of seed germination rate on water agar/paper and MS, but plantlets survival rate on MS agar under different stress was higher comparing to hydroponics.

Alginate-encapsulated vegetative buds have grown better on MS comparing to water.

You simply record the day when 75% of seeds have germinated, assuming all seeds are viable (which can be tested prior to the experiment using the flotation test) :)

Try different concentrations and then you will have superior data. But use viable seeds, or seeds that sink on the flotation test :)

Dear Aminu Moriki Ladan it depends of the number of parents involved in mating and their genotype. If either female and male parents are clones, F1 should be genetically uniform, at least external factors occur (mutations, contamination,...). However, if parents aren't clones, variations must be expected.

Visit kindly the following useful RG link:

Firstly, test seeds viability via tetrazalium.

The following links are also very useful: https://www.rimolgreenhouses.com/blog/pre-sprouting-seeds-101

Perhaps this article can be of your interest:

The answer to your question lies in the integrity of the cell wall. I just finished an experiment with one treatment having RWC > 100% and also high electrolyte leakage. In this case, FW > Sat weight and therefore, an RWC > 100%.

Terrestrial Orchids are well-suited for hydroponic growing, as they grow in moist, loose soil and need constant food and moisture to thrive, which is supplied with a hydroponic growing method. Selecting varieties suitable for the growing conditions in hydroponics improves chances of success.

Please see this.

Speed of germination= å x/N

i.e. X1/n1 + x2/n2 + x3/n3............. xn/Nn

Where, X = no. of normal seedling; N = no. of days

L. Marian Gonzàlez

This plant is either Albizia carbonaria (https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:8016-2) or Albizia julibrissin var. julibrissin (https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:77208306-1)

Thanks!

Agreed with Sajid Khan

Seed size and sometimes seed color can have a great variation in seed germination.

Thank you very much Elisa

Do not add zinc to soil since it inhibits seed germination :)

The question is very interesting

Coriander seeds usually atkes 2-3 weeks , depending upon quality . Most important is to ensure that they are not aged seeds and not for usage as spices...

Tetrazolium test very specifically mentions about the seed viability indications. depending upon the seed selected, your lab environment and tetrazolium test applied, you may like to develop your own protocol for your specific research

Description below may be useful:

i. Seed roll technique: Seeds may be rolled on 7-10 days old culture of fungi on the culture Petri plate, and Petri plate rotated in both clockwise and anticlockwise so that seed surface get covered by spore. The pathogen coated seeds are kept for germination on blotter paper, and then are kept in an incubator at 28- 30oC for about ten days.

ii. Seed soaking technique: This method is followed as paper towel technique. The fungus is cultured on Potato Dextrose Broth (PDB). Twenty ml of PDB is poured into 100 ml conical flasks and sterilized. The flasks are then inoculated and incubated for around ten days. The mycelial mat from the flask is removed and macerated in a warring blender along with distilled water for a minute. The inoculum is later collected in a beaker. In the meantime, seeds are immersed completely in the inoculum. These seeds are then placed side by side on a blotter paper (45cmx25cm with one fold) and are folded. The folded blotter papers are then placed in trays, and kept in an incubator at 28-30oC for around ten days.

For support: http://medcraveonline.com/APAR/APAR-06-00198.pdf

I do suggest the same mentioned by Do Bozena

Try with Agar 0.5% gel and a phosphate buffer pH 7.2.

GA is not a control; it is a treatment. You need to set up different concentrations of GA :)

Dear Paul Chandra Sekhar Chandra Sekhar Paul according to the ista "International Seed Testing Association" procedure distilled water is used for seed germination

I am interested , hope we will get some waiver for processing charges as MDPI are open access

i agree with Nitisha Srivastava

I think , this is the easiest method followed in all polyembryonic crops . Use of molecular markers is really difficult , where such mass scale production is required....

Zero tillage/Conservation agriculture

Dear Moaed,

If you are assessing the effect of soil on seed germination, so you should delicately dig and put the seed back quickly.

Do you mean MGT (Mean germination Time)?

Dear Ali,

I think this paper could guide you.

i had some experiment in salinity condition, probably in some pot, because of evaporation, the salinity deeply increased especially in light pot. also, in country we saw seed in the autumn( cold condition).

I agree with you, however cytotoxic analysis of certain nanoparticles on human cell line proved they're safe to be used, in addition some are effective in treating cancer cells

Temperature affects germination speed , but rate or percentage is affected by seed viability

Dear Noor,

Please look at this pdf:

Dear Noora,

It will decrease, may be by 50%. Soil characteristics is the main factor.

Sorry, no paper on comparative effect (pots/petri dish).

Regards

First, a simple seed germination test and be sure of the seed vigour and seed viability.

Thanks astawus

It depends on your seed species. Sometimes, cold is important to germination. Generally, seeds need cold temperature to reduce the effect of abscisic acid and enhance seed germination.

Dear Mr. Isam Al-madhagi,

Thank you for your answer. Our target is to obtain the plant from culturing the immature embryo. We can not extract only embryo from the seed that is the reason why we try to culture the half-seed. Our problem is that even we got the abnormal germination but after 3-4 times of subculture they will be brown and die.

You need to check for yourself :)

Please take a look at the following PDF attachments.

Anup Kumar Sarkar , you have pleinty of documents just make a research on google. Good luck

Dear Joel,

Thanks so much for your infromation!

Well dear Mohamad Issaoui , of course the need is to clone any of these plant species. However, in the starting question, there is no information as to WHY germinate seeds under in vitro conditions. To do what? They don’t germinate at all under in vivo conditions? Species rescue?

However, if ever it is to clone them, the worst thing that one can do is to germinate them. And that’s where the difference between Angiosperms and Gymnosperms come in the discussion. Any Angiosperm sp can do something after germination, what is not the case for Gymnosperms. And ontogeny has nothing to do in the story. It is a question of physiological age. Yes, physiology is the expression of genes through biochemistry, cytology, morphology, etc. Nothing new! Except for geneticists!

What is the point?

I think that Phenological studies in trees must be done every season by Foretsry department. The data could indicate several informations about climate change, impact of environmental conditions...

Freshly seeds can germinate easily. After a long time storage > 2 months, embryonic dormancy will settle and the seeds will be difficult to germinate.

25°C and you can go to 30°C for a fast germination.

Try only one minute in Sodium Hypochlorite at 0.5% followed by a goodd washing in sterilized water.

It depends on plant species. Just follow the links of María Beatriz Espinosa , ISTA gives a detailed procedure for each genus and species.

Regards

Dear Ashish, try to check the seeds viability using Tetrazolium.

t test if the data is normally distributed or chi squared test if it is not

Opuntia seeds imbibe water without disrupting the seed coat, therefore water soaking, although being an easy try, might not help by itself. I would try to remove a tiny fraction of the point of the seed (nail clipper might help) before puting them in a moistened substrate. You should also watch the temperature, it should not be too warm.

you can use the DMSO in higher concentration up to even 10% but it depend on your test.be careful about control test.

if your problem did not solve you can use DMF as mentioned above.

I appreciate your answers, please if you have chance read the explanation of the question.

From other you means which one ??

Abstracts:

1- Effect of diluted sea water on growth, yield, essential oil productivity and chemical composition of coriander (Coriandrum sativum L.) plants.

2- Efficiency of chemical, biological fertilizers and gibberellin on Coriandrum sativum L. under the condition of salinity and calcareous soil.

Apart from other environmental cues, its important to prevent exposure to relative humidity lower than 50% and lack of air exchange also prevent development of good leaves. We grown many of Arabidopsis accessions in small pots and prevented direct contact of leaves with the growing substrate using a black plastic sheet. When I grow them in rockwool or in vitro, the size of the leaf was considerably decreased. Have a look on the following paper:

Aliniaeifard S, van Meeteren U. 2014. Natural variation in stomatal response to closing stimuli among Arabidopsis thaliana accessions after exposure to low VPD as a tool to recognize the mechanism of disturbed stomatal functioning. Journal of Experimental Botany 65, 6529-6542.

see Daws, M.I., H.W. Pritchard, and J. Van Staden. 2008. Butenolide from plant-derived smoke functions as a strigolatone analogue: Evidence from parasitic weed germination. South African Journal of Botany 74:116-120

Not the plant holoparasitic family you are working with but may be of use