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Seed Germination - Science topic

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Normally F1 plants suppose to be uniform both genetically and phenotypically and start segregating at F2, but I notice some differences in seed germination and seedling vigor (all things being equal) WHY?
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Dear Aminu Moriki Ladan it depends of the number of parents involved in mating and their genotype. If either female and male parents are clones, F1 should be genetically uniform, at least external factors occur (mutations, contamination,...). However, if parents aren't clones, variations must be expected.
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How to breaking dormancy Eucalyptus deglupta ? some time ago I bought the seeds Eucalyptus deglupta, I tried to germinate the seeds, but after one week none of the seeds germinated , wheter the seeds is dormant? and how to break dormancy Euclyptus deglupta?
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I am working on tissue culture of an epiphytic orchid. The asymbiotic seed germination was screened in different basal media (MS, 1/2 MS, K-C, G-B5, Mitra, etc.) and later sub-cultured in best basal medium in combination with different PGRs (Auxins, Cytokinins, Chitosans, etc.) However, the growth of in-vitro cultures are very slow. What could be the reason for slow growth?
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Visit kindly the following useful RG link:
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I've received seeds of Arabidopsis col-0 and ws mutants for nitrate uptake, and they don't germinate.
Seed conditions
They're as old as 13 years and were kept in airtight plastic bag at room temperature.
Sterilisation
I sterilised them by shaking them for 5 minutes in a water solution 50% EtOH (v/v), 1.2% NaOCl (v/v, bleach), then rinsed them 3 times with EtOH 99%, and 3 times with autoclaved milliQ dH2O and left them dry on autoclaved filter paper under sterile hood. The floating seeds are removed during rinsing.
Sowing
I sowed them on MS/2 in Petri dishes and sterilised soil.
The ones on MS/2 were stratified at 4°C for 4 days, a second batch for 1 week.
Most genotypews have not germinated on either MS/2 nor soil.
I tried to add GA4+7 10 uM to the MS/2, but didn't induce germination.
Could you suggest any idea to try induce germination and flowering?
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Firstly, test seeds viability via tetrazalium.
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Most of the articles recommend an ex-situ approach in which we count a particular number of seeds and place them on a filter paper inside a petri dish. Next, we monitor the growth and germination of the seeds inside the petri dish. But I wonder if there is any in-situ method that allows us to directly determine the percentage of seed germination in Nursery beds.
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The main issue is storing seeds of tropical tree species is pathogens attacks, mainly insects. Which chemicals can be added to the seeds to decrease the amount of predated seeds?
Many thanks
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Perhaps this article can be of your interest:
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I finished my experiment RWC with 12-day-old transgenic soybean in normal treatment (watering). There was nothing wrong with sample collection, all plant was not dirty and I cleaned out the water left with tissue paper before the FRESH WEIGHT. After that, I sink deeply all plants in distilled water for 24 hours. And then, the TURGID WEIGHT was larger than FRESH WEIGHT, resulted that the RWC was larger than 100% (110-230%). I used the same electrical scale for this experiment. 
I'm wondering why this can happen and I don't know why? 
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The answer to your question lies in the integrity of the cell wall. I just finished an experiment with one treatment having RWC > 100% and also high electrolyte leakage. In this case, FW > Sat weight and therefore, an RWC > 100%.
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Hello everyone. I am curious whether it is possible to grow terrestrial orchids hydroponically under normal conditions, not sterile. Does anyone have such knowledge? I would wonder if Calanthe could be cultured that way after seed germination in sterile conditions. With a small scale culture system, hopefully we may be able to avoid plant viruses and promote growth.
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Terrestrial Orchids are well-suited for hydroponic growing, as they grow in moist, loose soil and need constant food and moisture to thrive, which is supplied with a hydroponic growing method. Selecting varieties suitable for the growing conditions in hydroponics improves chances of success.
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The use of natural products is an alternative for the control of pathogens associated with seeds, with the advantage of cost reduction and absence of environmental impact caused by pesticides.
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Seed germination
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Speed of germination= å x/N
i.e. X1/n1 + x2/n2 + x3/n3............. xn/Nn
Where, X = no. of normal seedling; N = no. of days
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I took some samples of a plant and their seeds and I leave in germination. when I make this process this plant start to make a smell like onions or I don´t know sulfur odor. I need to know the name of this plant, I can´t find the relationship of odor with this type plant.
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Hi all professor. Could you please tell me what cause this problem on melon. as you notice, some seeds germinated inside fruits. why?
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Agreed with Sajid Khan
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Hello everyone,
We are planning to study how plant growth promoting bacteria are affecting various plants (eg. wheat, barley, etc.). We would like to coat the seeds with bacteria. We prefer film coating. I can't find any proportions of seed, inoculum, carrier materials and sticking agents.
Could you please share the recipe you are using for your research? We prefer using cellulose or lime as a carrier material.
Thank you in advance.
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Hello.
I'm working with germination of various species of a genus, while analizyng the data I have found a correlation:
The bigger the seed of a species, the range of time the seeds germinate is wider.
The smaller the seed of a species, the range of time the seeds germinate is shorter.
Trying to explain this, i have a tought... This could be 'cause a bigger seed have more chances to resist dormancy and so, so they can have the "option" to germinate in diferent times, so the seedlings don;t compete between each other.
Does this make sense? Any one have a peper or some reference that back up this tought?
I'll love to hear your toughts and your help with the literature.
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Seed size and sometimes seed color can have a great variation in seed germination.
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For coexpression assay, i need a temporal transcriptome for the seed germination process of Arabidopsis thaliana.
Thank in advance for every help.
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Thank you very much Elisa
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What's the advantage and limitation of this method. I want to know more about the method about spike.
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Do not add zinc to soil since it inhibits seed germination :)
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During cryopreservation protocol for ultra dried seeds we determine contents of peroxide and superoxide. However, there are information related with hypothesis that the molecular mobility is very low almost cero.
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The question is very interesting
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Hii, I'm working on the seed germination assay of Coriander using the Petri dish method. I want to know what are the various factors that impact in seed germination of coriander and how many days it takes to germinate. If there is any protocol kindly share it with me.
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Coriander seeds usually atkes 2-3 weeks , depending upon quality . Most important is to ensure that they are not aged seeds and not for usage as spices...
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Hello, everyone!
I need to evaluate the increase/decrease of seeds' viability with tetrazolium test, if these seeds were previously exposed to nanoparticles. The species of my study is Capsicum annuum. Please, if you would be so kind, send me scientific articles that support your answer. Thanks a lot!
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Tetrazolium test very specifically mentions about the seed viability indications. depending upon the seed selected, your lab environment and tetrazolium test applied, you may like to develop your own protocol for your specific research
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Plant beneficial microbes can boost plant immunity and growth.
They are usually applied to young plants, but I want to apply them to seeds.
With tomato seeds its easy to apply fungal spores to the seeds. Vortex. Dry. Plant
With small Arabidopsis seeds (col 0) , its a bit more tricky. They are smaller, have less surface area and weigh around 20mg for 1000 seeds.
What techniques have you used to treat Arabidopsis seeds or other small seeds with microbial agents?
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Description below may be useful:
i. Seed roll technique: Seeds may be rolled on 7-10 days old culture of fungi on the culture Petri plate, and Petri plate rotated in both clockwise and anticlockwise so that seed surface get covered by spore. The pathogen coated seeds are kept for germination on blotter paper, and then are kept in an incubator at 28- 30oC for about ten days.
ii. Seed soaking technique: This method is followed as paper towel technique. The fungus is cultured on Potato Dextrose Broth (PDB). Twenty ml of PDB is poured into 100 ml conical flasks and sterilized. The flasks are then inoculated and incubated for around ten days. The mycelial mat from the flask is removed and macerated in a warring blender along with distilled water for a minute. The inoculum is later collected in a beaker. In the meantime, seeds are immersed completely in the inoculum. These seeds are then placed side by side on a blotter paper (45cmx25cm with one fold) and are folded. The folded blotter papers are then placed in trays, and kept in an incubator at 28-30oC for around ten days.
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We are working to determine the possible effect (if any) that different environmental parameters might have on seed germination and behaviour? I would like to know how I can go about doing this in an ex situ system.
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I do suggest the same mentioned by Do Bozena
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Hi!
I am looking for a recipe for a type of clear soil to see roots formation, but that would also resist/slightly resist fungal/bacterial contamination. It is a project for kids at a science center/museum.
I tried agar (no nutrient/bacteriological grade, 1% gel) with iba-k indole butyric acid, but the result is not clear enough to see through when we pour into a glass cup (it gets more translucent than clear). I tried Carbopol 940 with iba-k indole butyric acid, but this gives a very thick gel that cannot be poured, and traps a lot of huge bubbles when mixing/making it, making roots not very easy to see, although it is a very clear medium.
Any plant specialist out there have a suggestion?
Thank you very much!
Helene
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Try with Agar 0.5% gel and a phosphate buffer pH 7.2.
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Hi I am trying to establish a germination assay for Arabidopsis. I grew Arabidopsis seedling in MS medium and medium with Gibberellic acid (GA) as a positive control
However I didn't see any difference. What do you think I could change?
Medium: Murashige and skoog (4.4 g/l ) Sacarose (10 g/l) Agar (8 g/l)
GA Concentrations: 1 µM and 100µ M
I prepared a stock solution of 10 mM in water and I added to adjust the pH (NaOH 50%)
I sterilized the seedling and placed them in the medium immediately. I checked them after 72 h
Thank you :)
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GA is not a control; it is a treatment. You need to set up different concentrations of GA :)
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We are going to do a seed germination experiment but found that most of the previous research used distilled water as a control solution for seed germination. As distilled water is devoid of any nutrients then plasmolysis can occur during seed germination. Can anyone suggest what should be the perfect composition for the seed germination solution as an experimental control?
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Dear Paul Chandra Sekhar Chandra Sekhar Paul according to the ista "International Seed Testing Association" procedure distilled water is used for seed germination
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I have 14 species, 6 temperature settings and two treatments to examine germination responsiveness over 59 days. I want to start with some basic info for plotting (t50, Gmax etc). I want to compare species/populations and have installed R germinationmetrics Package but am having difficulty with the best set up to import my data....for example do I put all the 6 different temperatures in the one excel column alongside germination counts and treatments? I have 4 replicates per species per treatment per temperature. Can I compare or do I need to plot/analyses each species/pop individually?
All feedback received with thanks!
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I spent ages trying to figure out a way to do it! Happy to give you a hand if you need it, my email is johodges@csu.edu.au
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Hydrogen gas has effects on a range of physiological events in plants. It has been shown to have effects on seed germination, plant growth, and development. It has also been found to be involved in plant stress responses and to be protective against pathological abiotic stress challenges. Similarly, it also has beneficial effects during the post-harvest storage of crops. Therefore, its use in the agricultural setting has great potential as it appears to be safe, with no toxicity or harm to the environment.
This Special Issue aims to bring together a body of papers that focus on the current state-of-play of the molecular biology and possible uses of molecular hydrogen with plants. It is hoped that this Special Issue will highlight the future work which may be undertaken in this field and help to encourage researchers to investigate this exciting field further.
For more information please follow the URL, below.
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I am interested , hope we will get some waiver for processing charges as MDPI are open access
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Hello to all dear Researcher and scientist hope you are fine.
1.i need some precious suggestion that which parameters i select for my seed germination experiment.?
2.how to compare the different species seed germination rate.?
3. what are the methods of soaking time and sterilization of seeds.
need to recommend some good articles, methodology
positive response will be highly appreciated
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i agree with Nitisha Srivastava
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In Jamun there is no phenotypic differences in the growth of both nucellar and zygotic seedling also the point of initiation of these seedling are also same.
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I think , this is the easiest method followed in all polyembryonic crops . Use of molecular markers is really difficult , where such mass scale production is required....
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Crop seeds are sometimes exposed to drought stress
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Zero tillage/Conservation agriculture
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I want to know the scientific characterization of seed germination when I sow seeds in soil and I cant measure radicle length.
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Dear Moaed,
If you are assessing the effect of soil on seed germination, so you should delicately dig and put the seed back quickly.
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How we can calculate Dn?
Average duration required for germination :
ADG=Dn/n
Where, ADG= Average duration required for germination
n= number of seeds
Dn= number of seeds germinated in days
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Do you mean MGT (Mean germination Time)?
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hello everyone, I want to know the relationship of seed oil contents (Gadoleic acid, erucic acid, thioglycoside) with seed germination and drought stress in plants?
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Dear Ali,
I think this paper could guide you.
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First , I have an experiment with 24 pots where 10 quinoa seeds were swoon in each pot , unfortunately in the pots that were irrigated 10ds/m-1 and 15ds/m-1 their were low germination and in 15ds/m-1 nothing germinated . I was wondering what are the factors that might have affected my experiment , soil salinity wasn't above what quinoa can tolerate , soil PH is also at the level quinoa can tolerate
I repeated the experiment , in the same pots that were irrigated with saline water , first I added fresh water for 7 days and then 7 days with saline water , 2 pots out of 6 germinated in 10ds/m-1 even with fresh water irrigation first , nothing germinated in 15 NaCl , I thought if it was the salinity all pots won't germinate but they have almost the same level of soil salinity , irrigated with 200 mL each , so I don't know why the won't germinate or why only 2 pots out of 6 germinated ? what other factors that can affect quinoa seed germination ?
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i had some experiment in salinity condition, probably in some pot, because of evaporation, the salinity deeply increased especially in light pot. also, in country we saw seed in the autumn( cold condition).
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As nanoparticles are actually toxic for human beings in many aspects then why are finding their application in medical biology if we can't take them as a drug or by any other mean.
In the same way, nanoparticles show very good effect on seed germination, but it is always a concern to use that cultivation as an intake by human beings, as scientist says these nanoparticles are not safe for us.
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I agree with you, however cytotoxic analysis of certain nanoparticles on human cell line proved they're safe to be used, in addition some are effective in treating cancer cells
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Does soil temperature affect seed germination , germination rate or percentage ?
Provide two scientific articles to support ur answer (Just the link)
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Temperature affects germination speed , but rate or percentage is affected by seed viability
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Does seed quality affect seed germination , germination rate or percentage ?
Provide two scientific articles to support ur answer (Just the link)
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I have Q5 Quinoa seeds that had 60% germination rate in petri dishes , how much will be the expected germination rate in pots ? will it decrease and if it decrease how much is the percent of decrease ?
If you can provide me with scientific papers or sources that support the answer please
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Dear Noora,
It will decrease, may be by 50%. Soil characteristics is the main factor.
Sorry, no paper on comparative effect (pots/petri dish).
Regards
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Hi, I'm grateful to someone who helps me.
Salvia multicaulis seeds treatmented in 500 ppm GA3 for one night and then placed in the germinator, also the seeds placed in the MS and B5 medium with 0.5 g / l GA3, after 5 days no germination was observed.
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First, a simple seed germination test and be sure of the seed vigour and seed viability.
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When compared to control do primmed seeds show late germination...if so what is the reason??? Please reply
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Thanks astawus
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What are the methods of reducing the negative impact of cold stress on seed germination?
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It depends on your seed species. Sometimes, cold is important to germination. Generally, seeds need cold temperature to reduce the effect of abscisic acid and enhance seed germination.
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Dear all,
I have a question related to embryo rescue. We conducted experiment on wild Cucumis species.When we cultured the half-seed contained embryo, after 3-4 weeks, some seeds germinated but looked abnormal which means we got the plants either formed root or formed shoot . And a white part is always appeared (Please see the attached photos). Then we continued sub-culturing but could not get the normal plants. We think during embryogenesis duration, auxin might be insufficient. We cultured on MS medium without hormone.
Should we try with medium supplemented with auxin?
I am looking forward your comments and advice.
Thank you so much.
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Dear Mr. Isam Al-madhagi,
Thank you for your answer. Our target is to obtain the plant from culturing the immature embryo. We can not extract only embryo from the seed that is the reason why we try to culture the half-seed. Our problem is that even we got the abnormal germination but after 3-4 times of subculture they will be brown and die.
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Seed Germination and Seedling Survival Percentage of Shorea robusta Gaertn.f. in Buffer Areas of Similipal Biosphere Reserve, Odisha, India.
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You need to check for yourself :)
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Could anyone help me to improve Citrullus colocynthis (Bitter apple) seeds germination or break its seed dormancy?
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Please take a look at the following PDF attachments.
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Salt stress during seed germination.
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Anup Kumar Sarkar , you have pleinty of documents just make a research on google. Good luck
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Have you any information about seed germination of Gundelia ssp in Astraceae Family?
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Dear Joel,
Thanks so much for your infromation!
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Hi,
Normally, the success of seed germination through in vitro culture is influenced by several factors such as medium composition, seed origin (plant species), seed maturity...
The success to ensure normal germination and development from in vitro cultured seeds of perennial lignified plants is very limited. Why??
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Well dear Mohamad Issaoui , of course the need is to clone any of these plant species. However, in the starting question, there is no information as to WHY germinate seeds under in vitro conditions. To do what? They don’t germinate at all under in vivo conditions? Species rescue?
However, if ever it is to clone them, the worst thing that one can do is to germinate them. And that’s where the difference between Angiosperms and Gymnosperms come in the discussion. Any Angiosperm sp can do something after germination, what is not the case for Gymnosperms. And ontogeny has nothing to do in the story. It is a question of physiological age. Yes, physiology is the expression of genes through biochemistry, cytology, morphology, etc. Nothing new! Except for geneticists!
What is the point?
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Tree phenology generally refer to calendar of events taking place in life history of a tree. It includes from leaves emergence to fruit or seed setting all for a silviculturist. I want to focus on phenology of flowering and fruiting of trees vis and vis seed quality, determination of seed maturity indices and finally impact on viability/ seed germination. Is there any such studies or corelation has been develop for tropical trees specially Anthocephalus cadamba syn. Neolamarckia cadamba . If such studies carried out any other tree species are also welcome to share.
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I think that Phenological studies in trees must be done every season by Foretsry department. The data could indicate several informations about climate change, impact of environmental conditions...
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We launched a study on germination of cork oak (Quercus suber). Our tests failed because of contamination, despite the sterilization of the acorns by the bleach solution. How to eliminate these contaminations that block germination? What is the best way to germinate acorns? and if you have any documents on this subject. Thank you in advance for all your help.
Benmahioul
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Freshly seeds can germinate easily. After a long time storage > 2 months, embryonic dormancy will settle and the seeds will be difficult to germinate.
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FOR GROWING OF CHICKPEA KABULI VARIETY.
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25°C and you can go to 30°C for a fast germination.
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I have tried using ethanol and bleach at different concentrations and in combinations and separate as well. But the germination is affected (reduced to around 10%) and sometimes kills the seed. The seed coat soaks up water very quickly and breaks which might be resulting in poor germination. Dry seeds germinate quicker than wet soybean seeds. Can anybody please suggest an efficient way to surface sterilize the soybean seeds without decreasing the germination rate?
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Try only one minute in Sodium Hypochlorite at 0.5% followed by a goodd washing in sterilized water.
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Paper for carrying out germination test.
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It depends on plant species. Just follow the links of María Beatriz Espinosa , ISTA gives a detailed procedure for each genus and species.
Regards
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I have problem in germinating elephant apple seed because of hard seed cover. Please suggest me how can i germinate it? Seeds are mucilaginous in nature and have hair on its surface.
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Dear Ashish, try to check the seeds viability using Tetrazolium.
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I did a germination study with different crops like Radish, Corn and Basil. Can someone suggest me how to analyze the Germination Index (GI) data statistically? I currently have access to JMP software. I referred this formula to calculate GI
GI= (10*n1)+(9*n2)....+(1*n10) (Kader, 2005)
where n1 through n10 is the number of seeds germinated on the first to 10th days and
10,9,8,7 are weights given to the number of seeds germinated on each day.
Thank you :)
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t test if the data is normally distributed or chi squared test if it is not
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Dear colleagues
In most articles, sulfuric acid or GA3 were used to break the dormancy and improve germination of cactus (Opuntia) seeds. What other materials can be used to increase germination of cactus seeds?
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Opuntia seeds imbibe water without disrupting the seed coat, therefore water soaking, although being an easy try, might not help by itself. I would try to remove a tiny fraction of the point of the seed (nail clipper might help) before puting them in a moistened substrate. You should also watch the temperature, it should not be too warm.
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I work on plant extract for seed germination assay. However, I face some issues on dissolving my plant extract. Initially, I try to use distilled water to dissolve it but my plant extract is not dissolved completely. So, I am planning to use DMSO and dilute DMSO at 1% using distilled water to dissolve my sample extract. May I know is this concentration toxic to seed germination? Or dissolve sample using pure DMSO then only do serial dilution? Any suggestion? Thanks in advance!
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you can use the DMSO in higher concentration up to even 10% but it depend on your test.be careful about control test.
if your problem did not solve you can use DMF as mentioned above.
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  • I found the fungi was growing when the Brachypodium seed was germination. I got these two brachypodium phenotypes, anybody know it is fungi pathogen cause this phenotype? The attachment is the phenotype, the tip of the leaves were shrivel, and the botten of the leaves had some spot.
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Practical aspects of planting mahogany by seed
Patricia Negreros Castillo patri_nc@yahoo.com
We have found that mahogany (Swietenia macrophylla), one of the most valuable timber trees in the world, seed germinate and the resulting seedlings do well when sown in a slash and burn field during the last cropping year before abandonment (fallowing) of the field (1). As mahogany seed require about 30 days to emerge, a high percentage of seed would be predated between the sowing date and the time of emergence (2) As a result, many seed would need to be sown to get a reasonable number of established seedlings, and their distribution would likely be very irregular.
A solution would be to reduce the number of days between sowing and emergence. Planting seedlings would be a solution, but the fields are often a considerable distance from any road, and 2 bagged seedlings weigh about 1 kg which is the weight of about 2000 mahogany seed. We think that by dampening the seed for some days before sowing, the days to emergence after sowing would be reduced and predation reduced.
We would like to communicate with individuals who know about planting seed that have been treated for extended periods of time before seeding and individuals with suggestions about what we might do. We know that shading and moisture improve emergence. (3)
1 – Negreros-Castillo, P., I. Martinez-Salazar, C. Alvarez Aquino, A. Navarro Martinez, C.W. Mize. 2018. Survival and growth of Swietenia macrophylla seedlings from seeds sown into slash and burn fields in Quintana Roo, Mexico. Bois Et Forets Des Tropiques 336. 10 p.
2 – Negreros-Castillo, P., I. Martinez-Salazar, K.F. Kellner, C.W. Mize, R.K. Swihart, M.A. Navarro-Martinez. 2016. Bois et Forets des Tropiques 329. 10 p.
3 – Morris, M., P. Negreros-Castillo, and C. Mize. 2000. Sowing date, shade, and irrigation affect big-leaf mahogany (Swietenia macrophylla King). Forest Ecology and Management 132:173-181.
____________________________________
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I appreciate your answers, please if you have chance read the explanation of the question.
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Nanoparticles toxicity on seed germination
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Nanoparticles toxicity on seed germination
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From other you means which one ??
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I need information about biostimulators for seed germination, plantlet growth and plant development of following medicinal plants:
1. Nigella sativa,
2. Trigonella foenum,
3. Coriandrum sativum,
4. Rhus coriaria.
Also, I would like to have some papers about development of stress tolerance of these plants both in vitro and in vivo conditions.
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Abstracts:
1- Effect of diluted sea water on growth, yield, essential oil productivity and chemical composition of coriander (Coriandrum sativum L.) plants.
2- Efficiency of chemical, biological fertilizers and gibberellin on Coriandrum sativum L. under the condition of salinity and calcareous soil.
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Hi colleagues, I have Arabidopsis thaliana wild type (Columbia) seeds. Seeds germinate well and their seedlings with first leaves (green) develop 1-2 cm long. The first leaves of seedlings are beautiful and green. However, seedlings do not develop after this stage and they remain very weak. Although I have tried many ways (Rockwool, hydroponics, soil, Petri dishes with Hoagland or MS), the seedlings do not grow and are the same even after 21 days.
I wonder what the problem is.
Thank you in advance for your contribution
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Apart from other environmental cues, its important to prevent exposure to relative humidity lower than 50% and lack of air exchange also prevent development of good leaves. We grown many of Arabidopsis accessions in small pots and prevented direct contact of leaves with the growing substrate using a black plastic sheet. When I grow them in rockwool or in vitro, the size of the leaf was considerably decreased. Have a look on the following paper:
Aliniaeifard S, van Meeteren U. 2014. Natural variation in stomatal response to closing stimuli among Arabidopsis thaliana accessions after exposure to low VPD as a tool to recognize the mechanism of disturbed stomatal functioning. Journal of Experimental Botany 65, 6529-6542.
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I now try to writing research proposal about germination of seed from parasitic plant (in this case, i try to germinate the rafflesia seeds). If anyone here have some experiences in germination of parasitic plant seeds especially holoparasitic plant like rafflesia, i would like to get some advice and maybe technique to do it, and if already have protocol to do it, would you like to share with me.
thank you in advance. hope advice, comment and suggestion from all of you will help me to improve this research and i can start working with this project.
best wishes,
idris
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see Daws, M.I., H.W. Pritchard, and J. Van Staden. 2008. Butenolide from plant-derived smoke functions as a strigolatone analogue: Evidence from parasitic weed germination. South African Journal of Botany 74:116-120
Not the plant holoparasitic family you are working with but may be of use
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Suppose we are checking the effect of salinity on seed germination and we are measuring the effect by observing the lengths of shoot and root after a certain no. of days.
In order to see the salt concentration which gives the most significant difference between the control and the test samples, should we consider a non-parametric or a parametric test?
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Hi,
I want to germinate transgenic rice seeds (T2 generation) in hygromycin solution to see the segregation ratio of 7 events. Did anyone tried this before??
Will appreciate any comments on this.
thanks
Danny
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Dear Hossein
thanks for the link, and comments, I am testing for homozygocity and no other analysis
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I am performing seed germination study of fenugreek in controlled environment(lab).I want to use to germination chamber for the experiment. I want to know the temperature and relative humidity required for germination experiment of fenugreek.
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To my mind, Temp. in the range of 25 to 35 degree is congenial for majority of the seeds to germinate since, metabolic activities of the cell is triggered at this temperature. humidity more than 70 % be beneficial. Personally suggesting variable temperature for day and night.
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I am looking for studies or key words that would help me explore the body of literature that tests whether seed germination is an inherently social or solitary activity in a plant species. Any advice or leads would be appreciated
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No tengo experiencias personales a su inquietud.No obstante pienso que la germinación de una semillgerminaciona va a estar determinada en en su vialidad,poder de germinación del lote y las condiciones agroclimaticas que perduren en la región que se siembren.Es importante proteger las semillas antes de sembrar con productos naturales o químicos para protegerlos del ataque de plagas y enfermedades
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I need these concentrations to prime my seeds for germination and emergence improvement.
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Dear Promise
I have sent three files.
You can use Excel file for calculating water potentials with PEG 8000 AND 6000.
Best regards
Farshid
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seeds will treat with  KNO3 for 24hr .can we grow these seeds in ms media zero?
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No problem, you can do it. After priming it is better to grow the seeds in 1/2 MS media so this media will promote the root growth and you will have finaly more powerful plants. Good luck.
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we have done red cadamba seed germination in vitro by treating with fungicide and double sterilization with 50 % Clorox and denatured alcohol and inoculate it in MS media with hormone but it shows low germination rate even after a month .
please advice me on how to increase the germination rate and reduce the contamination rate
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Hello
Are your seeds alive ? Which hormone and which fungicide are you using ? Why using MS medium rather gelled water or moisted filter paper ? Is there any seed dormancy for this tropical tree ?
Regards
MJ
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I found some grey/black spots on a filter paper when plant seeds were germinated. It is weird that the spots are not close to the seeds. They should not be derived from the deionized water because I set a control without seeds and spots did not appear there. What shuld be the reasons and how to deal with it? Thanks!
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Dear Mr.Zhou,
I deem the sterilization protocol too mild.
I seal the filter paper in a bag and autoclave it for 20' at 121°C reaching 300 kPa.
I shake the seeds for 8' in 400-1000 uL of a solution 12% sodium hypochlorite + Triton X-100 in proportion 1000:1, then centrifuge, remove the solution using 10 uL tip on a 1000 uL one in order to avoid taking the seeds.
Then I wash them with dH2O five times before spreading them on the filter paper with the last washing water and let them dry before use.
All done under under laminar air flow hood.
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Maize sterilization
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70% ethanol (1 min.) followed by 5% sodium hypochlorite (5 min.) and rinse several times with sterile water before sowing for germination
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I am working on Okra tissue culture but i am facing problem about okra seed germination , for seed treatment i am using HgCl2 0.1% for 3 minutes and after washing with dd water use 75 % Ethanol for 15 seconds and washed with dd water for 5 times and soaked in water for one night and next day put in 1/2 MS media ( 15 g sucrose, 8 g agar and 2.37 g MS powder media for 1 litter ) and place in dark for 1 to 2 days until the plumes come out and then placed in florescent light . i have tried many times, i have changed seed from different companies but some time good germination and next time 0%. can anybody help me about this problem ?
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Dear Hafiz
In addition to the toxicity of mercury chloride, okra shows a particular kind of seed dormancy, called delayed permeability, caused by the structure of the seed coat and particularly by the chalazal plug. The combination of temperature and time (40°C for 4 days and 100% RH) were pointed out to solve the dormancy.
Nada, E., Lotito, S. and Quagliotti, L. (1994). SEED TREATMENTS AGAINST DORMANCY IN OKRA (ABELMOSCHUS ESCULENTUS (L.) MOENCH).. Acta Hortic. 362, 133-140
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This specie of family Arecaceae, known as vegetal ivory, is derived from a hardness of its seeds. Seeds germinate approximately 8 months after sowing.
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Dear Marcus,
Please check this PDF attachment.
Good luck!
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In scientific resources is noted that the seed coat mucilage only physical barrier to seed germination (preventing the diffusion of oxygen).
Is it not possible to create a new chemical compound between oxygen and mucilage that inhibits of seed germination and embryo growth?
It's not possible?
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Dear Maria:
Thank you for the answer
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hello every one,
Hope reading my question will all be in good health, can some one tell me why my seeds not germinate? actually i planted my alfalfa seeds on 12 June 2018 in sole cropping and inter-cropping with maize but the maize are now at 4-5 leaves stage and the alfalfa are not yet germinate, after two weeks then i reseeded alfalfa but again after three days did not notice any sign of germination could you please help me what are the key problems and their solutions.
Thank you
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Intact seeds of Cistaceae , Malvaceae, Fabaceae and Convolvulaceae species generally have physical dormancy. The treatments such as mechanical scarification, acid scarification and dry/wet heat can be used to break physical dormancy.
For more details, see below articles.
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I found from an literature that ft-1 mutant has reduced germination rate, while the insoluble tannin content in the seeds coat is high, so why is that?
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Tannin content is known to be correlated with low seed germination. For example, see:
doi:10.2134/agronj1970.00021962006200060051x
Influence of Tannin Content on Preharvest seed Germination in Sorghum
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Hello, can I get help on the rate of ethrel to use in peanuts to break seed dormancy, Is it the best way to soak or just spray the seeds prior sowing?. Is convenient to use ethrel only or it works well with other growth regulators?
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Thank you all. The best application rate and application method in my experiment was 0.05%(w/v) ethrel and 0.625ml/l tebuconazole-carbendazim fungicide mixture. When sprayed directly into seeds in the furrow and immediately cover with soil, this displayed high efficiency compared to other methods I have tested. Once more I thank you all for taking time to help me out.
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I am working on propagation of Gmelina arborea ( Gamar/ sivan). The species is widely explored for agroforestry purposes but quality planting material is still a constraint. I not come across much seed and seed propagation studies in the species. Can anybody suggest/ provide some references/ article on  Gmelina arborea seed and seed/ vegetative  propagation studies.
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A study was attempted to evolve method for mass multiplication of mature ortets of G. arborea involving rooting of miniature bud sprouts.
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We evaluated the response of tree seeds germination to application of something like "growth stimulator". Control, Low, Medium and High concentrations were selected. The thing is, that Low and High concentration of chemical significantly affected seed germination, while Medium concentration did not. Does anybody encountered similar behavior of some kind of chemicals/drugs/something?
Thanks a lot. 
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This phenomenon is related to interactions of species and the concentration of growth regulator. In high or low concentrations, growth regulator effects may be inhibitors or stimulants.
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I am working with Festuca rubra and Agrostis capilaris growing in contaminated soils, but the seeds have very low germination rate. Currently I’m comparing germination and growth of this seeds vs healthy seeds. However it would be interesting to compare seeds vs clonal reproduction of the same genotype, but I haven’t found any paper a