Questions related to Seed Germination
Normally F1 plants suppose to be uniform both genetically and phenotypically and start segregating at F2, but I notice some differences in seed germination and seedling vigor (all things being equal) WHY?
How to breaking dormancy Eucalyptus deglupta ? some time ago I bought the seeds Eucalyptus deglupta, I tried to germinate the seeds, but after one week none of the seeds germinated , wheter the seeds is dormant? and how to break dormancy Euclyptus deglupta?
I am working on tissue culture of an epiphytic orchid. The asymbiotic seed germination was screened in different basal media (MS, 1/2 MS, K-C, G-B5, Mitra, etc.) and later sub-cultured in best basal medium in combination with different PGRs (Auxins, Cytokinins, Chitosans, etc.) However, the growth of in-vitro cultures are very slow. What could be the reason for slow growth?
I've received seeds of Arabidopsis col-0 and ws mutants for nitrate uptake, and they don't germinate.
They're as old as 13 years and were kept in airtight plastic bag at room temperature.
I sterilised them by shaking them for 5 minutes in a water solution 50% EtOH (v/v), 1.2% NaOCl (v/v, bleach), then rinsed them 3 times with EtOH 99%, and 3 times with autoclaved milliQ dH2O and left them dry on autoclaved filter paper under sterile hood. The floating seeds are removed during rinsing.
I sowed them on MS/2 in Petri dishes and sterilised soil.
The ones on MS/2 were stratified at 4°C for 4 days, a second batch for 1 week.
Most genotypews have not germinated on either MS/2 nor soil.
I tried to add GA4+7 10 uM to the MS/2, but didn't induce germination.
Could you suggest any idea to try induce germination and flowering?
Most of the articles recommend an ex-situ approach in which we count a particular number of seeds and place them on a filter paper inside a petri dish. Next, we monitor the growth and germination of the seeds inside the petri dish. But I wonder if there is any in-situ method that allows us to directly determine the percentage of seed germination in Nursery beds.
The main issue is storing seeds of tropical tree species is pathogens attacks, mainly insects. Which chemicals can be added to the seeds to decrease the amount of predated seeds?
I finished my experiment RWC with 12-day-old transgenic soybean in normal treatment (watering). There was nothing wrong with sample collection, all plant was not dirty and I cleaned out the water left with tissue paper before the FRESH WEIGHT. After that, I sink deeply all plants in distilled water for 24 hours. And then, the TURGID WEIGHT was larger than FRESH WEIGHT, resulted that the RWC was larger than 100% (110-230%). I used the same electrical scale for this experiment.
I'm wondering why this can happen and I don't know why?
Hello everyone. I am curious whether it is possible to grow terrestrial orchids hydroponically under normal conditions, not sterile. Does anyone have such knowledge? I would wonder if Calanthe could be cultured that way after seed germination in sterile conditions. With a small scale culture system, hopefully we may be able to avoid plant viruses and promote growth.
The use of natural products is an alternative for the control of pathogens associated with seeds, with the advantage of cost reduction and absence of environmental impact caused by pesticides.
I took some samples of a plant and their seeds and I leave in germination. when I make this process this plant start to make a smell like onions or I don´t know sulfur odor. I need to know the name of this plant, I can´t find the relationship of odor with this type plant.
We are planning to study how plant growth promoting bacteria are affecting various plants (eg. wheat, barley, etc.). We would like to coat the seeds with bacteria. We prefer film coating. I can't find any proportions of seed, inoculum, carrier materials and sticking agents.
Could you please share the recipe you are using for your research? We prefer using cellulose or lime as a carrier material.
Thank you in advance.
I'm working with germination of various species of a genus, while analizyng the data I have found a correlation:
The bigger the seed of a species, the range of time the seeds germinate is wider.
The smaller the seed of a species, the range of time the seeds germinate is shorter.
Trying to explain this, i have a tought... This could be 'cause a bigger seed have more chances to resist dormancy and so, so they can have the "option" to germinate in diferent times, so the seedlings don;t compete between each other.
Does this make sense? Any one have a peper or some reference that back up this tought?
I'll love to hear your toughts and your help with the literature.
For coexpression assay, i need a temporal transcriptome for the seed germination process of Arabidopsis thaliana.
Thank in advance for every help.
During cryopreservation protocol for ultra dried seeds we determine contents of peroxide and superoxide. However, there are information related with hypothesis that the molecular mobility is very low almost cero.
Hii, I'm working on the seed germination assay of Coriander using the Petri dish method. I want to know what are the various factors that impact in seed germination of coriander and how many days it takes to germinate. If there is any protocol kindly share it with me.
I need to evaluate the increase/decrease of seeds' viability with tetrazolium test, if these seeds were previously exposed to nanoparticles. The species of my study is Capsicum annuum. Please, if you would be so kind, send me scientific articles that support your answer. Thanks a lot!
Plant beneficial microbes can boost plant immunity and growth.
They are usually applied to young plants, but I want to apply them to seeds.
With tomato seeds its easy to apply fungal spores to the seeds. Vortex. Dry. Plant
With small Arabidopsis seeds (col 0) , its a bit more tricky. They are smaller, have less surface area and weigh around 20mg for 1000 seeds.
What techniques have you used to treat Arabidopsis seeds or other small seeds with microbial agents?
We are working to determine the possible effect (if any) that different environmental parameters might have on seed germination and behaviour? I would like to know how I can go about doing this in an ex situ system.
I am looking for a recipe for a type of clear soil to see roots formation, but that would also resist/slightly resist fungal/bacterial contamination. It is a project for kids at a science center/museum.
I tried agar (no nutrient/bacteriological grade, 1% gel) with iba-k indole butyric acid, but the result is not clear enough to see through when we pour into a glass cup (it gets more translucent than clear). I tried Carbopol 940 with iba-k indole butyric acid, but this gives a very thick gel that cannot be poured, and traps a lot of huge bubbles when mixing/making it, making roots not very easy to see, although it is a very clear medium.
Any plant specialist out there have a suggestion?
Thank you very much!
Hi I am trying to establish a germination assay for Arabidopsis. I grew Arabidopsis seedling in MS medium and medium with Gibberellic acid (GA) as a positive control
However I didn't see any difference. What do you think I could change?
Medium: Murashige and skoog (4.4 g/l ) Sacarose (10 g/l) Agar (8 g/l)
GA Concentrations: 1 µM and 100µ M
I prepared a stock solution of 10 mM in water and I added to adjust the pH (NaOH 50%)
I sterilized the seedling and placed them in the medium immediately. I checked them after 72 h
Thank you :)
We are going to do a seed germination experiment but found that most of the previous research used distilled water as a control solution for seed germination. As distilled water is devoid of any nutrients then plasmolysis can occur during seed germination. Can anyone suggest what should be the perfect composition for the seed germination solution as an experimental control?
I have 14 species, 6 temperature settings and two treatments to examine germination responsiveness over 59 days. I want to start with some basic info for plotting (t50, Gmax etc). I want to compare species/populations and have installed R germinationmetrics Package but am having difficulty with the best set up to import my data....for example do I put all the 6 different temperatures in the one excel column alongside germination counts and treatments? I have 4 replicates per species per treatment per temperature. Can I compare or do I need to plot/analyses each species/pop individually?
All feedback received with thanks!
Hydrogen gas has effects on a range of physiological events in plants. It has been shown to have effects on seed germination, plant growth, and development. It has also been found to be involved in plant stress responses and to be protective against pathological abiotic stress challenges. Similarly, it also has beneficial effects during the post-harvest storage of crops. Therefore, its use in the agricultural setting has great potential as it appears to be safe, with no toxicity or harm to the environment.
This Special Issue aims to bring together a body of papers that focus on the current state-of-play of the molecular biology and possible uses of molecular hydrogen with plants. It is hoped that this Special Issue will highlight the future work which may be undertaken in this field and help to encourage researchers to investigate this exciting field further.
For more information please follow the URL, below.
Hello to all dear Researcher and scientist hope you are fine.
1.i need some precious suggestion that which parameters i select for my seed germination experiment.?
2.how to compare the different species seed germination rate.?
3. what are the methods of soaking time and sterilization of seeds.
need to recommend some good articles, methodology
positive response will be highly appreciated
In Jamun there is no phenotypic differences in the growth of both nucellar and zygotic seedling also the point of initiation of these seedling are also same.
I want to know the scientific characterization of seed germination when I sow seeds in soil and I cant measure radicle length.
hello everyone, I want to know the relationship of seed oil contents (Gadoleic acid, erucic acid, thioglycoside) with seed germination and drought stress in plants?
First , I have an experiment with 24 pots where 10 quinoa seeds were swoon in each pot , unfortunately in the pots that were irrigated 10ds/m-1 and 15ds/m-1 their were low germination and in 15ds/m-1 nothing germinated . I was wondering what are the factors that might have affected my experiment , soil salinity wasn't above what quinoa can tolerate , soil PH is also at the level quinoa can tolerate
I repeated the experiment , in the same pots that were irrigated with saline water , first I added fresh water for 7 days and then 7 days with saline water , 2 pots out of 6 germinated in 10ds/m-1 even with fresh water irrigation first , nothing germinated in 15 NaCl , I thought if it was the salinity all pots won't germinate but they have almost the same level of soil salinity , irrigated with 200 mL each , so I don't know why the won't germinate or why only 2 pots out of 6 germinated ? what other factors that can affect quinoa seed germination ?
As nanoparticles are actually toxic for human beings in many aspects then why are finding their application in medical biology if we can't take them as a drug or by any other mean.
In the same way, nanoparticles show very good effect on seed germination, but it is always a concern to use that cultivation as an intake by human beings, as scientist says these nanoparticles are not safe for us.
Does soil temperature affect seed germination , germination rate or percentage ?
Provide two scientific articles to support ur answer (Just the link)
Does seed quality affect seed germination , germination rate or percentage ?
Provide two scientific articles to support ur answer (Just the link)
I have Q5 Quinoa seeds that had 60% germination rate in petri dishes , how much will be the expected germination rate in pots ? will it decrease and if it decrease how much is the percent of decrease ?
If you can provide me with scientific papers or sources that support the answer please
Hi, I'm grateful to someone who helps me.
Salvia multicaulis seeds treatmented in 500 ppm GA3 for one night and then placed in the germinator, also the seeds placed in the MS and B5 medium with 0.5 g / l GA3, after 5 days no germination was observed.
I have a question related to embryo rescue. We conducted experiment on wild Cucumis species.When we cultured the half-seed contained embryo, after 3-4 weeks, some seeds germinated but looked abnormal which means we got the plants either formed root or formed shoot . And a white part is always appeared (Please see the attached photos). Then we continued sub-culturing but could not get the normal plants. We think during embryogenesis duration, auxin might be insufficient. We cultured on MS medium without hormone.
Should we try with medium supplemented with auxin?
I am looking forward your comments and advice.
Thank you so much.
Seed Germination and Seedling Survival Percentage of Shorea robusta Gaertn.f. in Buffer Areas of Similipal Biosphere Reserve, Odisha, India.
Normally, the success of seed germination through in vitro culture is influenced by several factors such as medium composition, seed origin (plant species), seed maturity...
The success to ensure normal germination and development from in vitro cultured seeds of perennial lignified plants is very limited. Why??
Tree phenology generally refer to calendar of events taking place in life history of a tree. It includes from leaves emergence to fruit or seed setting all for a silviculturist. I want to focus on phenology of flowering and fruiting of trees vis and vis seed quality, determination of seed maturity indices and finally impact on viability/ seed germination. Is there any such studies or corelation has been develop for tropical trees specially Anthocephalus cadamba syn. Neolamarckia cadamba . If such studies carried out any other tree species are also welcome to share.
We launched a study on germination of cork oak (Quercus suber). Our tests failed because of contamination, despite the sterilization of the acorns by the bleach solution. How to eliminate these contaminations that block germination? What is the best way to germinate acorns? and if you have any documents on this subject. Thank you in advance for all your help.
I have tried using ethanol and bleach at different concentrations and in combinations and separate as well. But the germination is affected (reduced to around 10%) and sometimes kills the seed. The seed coat soaks up water very quickly and breaks which might be resulting in poor germination. Dry seeds germinate quicker than wet soybean seeds. Can anybody please suggest an efficient way to surface sterilize the soybean seeds without decreasing the germination rate?
I did a germination study with different crops like Radish, Corn and Basil. Can someone suggest me how to analyze the Germination Index (GI) data statistically? I currently have access to JMP software. I referred this formula to calculate GI
GI= (10*n1)+(9*n2)....+(1*n10) (Kader, 2005)
where n1 through n10 is the number of seeds germinated on the first to 10th days and
10,9,8,7 are weights given to the number of seeds germinated on each day.
Thank you :)
In most articles, sulfuric acid or GA3 were used to break the dormancy and improve germination of cactus (Opuntia) seeds. What other materials can be used to increase germination of cactus seeds?
I work on plant extract for seed germination assay. However, I face some issues on dissolving my plant extract. Initially, I try to use distilled water to dissolve it but my plant extract is not dissolved completely. So, I am planning to use DMSO and dilute DMSO at 1% using distilled water to dissolve my sample extract. May I know is this concentration toxic to seed germination? Or dissolve sample using pure DMSO then only do serial dilution? Any suggestion? Thanks in advance!
- I found the fungi was growing when the Brachypodium seed was germination. I got these two brachypodium phenotypes, anybody know it is fungi pathogen cause this phenotype? The attachment is the phenotype, the tip of the leaves were shrivel, and the botten of the leaves had some spot.
Practical aspects of planting mahogany by seed
Patricia Negreros Castillo email@example.com
Carl Mize firstname.lastname@example.org
We have found that mahogany (Swietenia macrophylla), one of the most valuable timber trees in the world, seed germinate and the resulting seedlings do well when sown in a slash and burn field during the last cropping year before abandonment (fallowing) of the field (1). As mahogany seed require about 30 days to emerge, a high percentage of seed would be predated between the sowing date and the time of emergence (2) As a result, many seed would need to be sown to get a reasonable number of established seedlings, and their distribution would likely be very irregular.
A solution would be to reduce the number of days between sowing and emergence. Planting seedlings would be a solution, but the fields are often a considerable distance from any road, and 2 bagged seedlings weigh about 1 kg which is the weight of about 2000 mahogany seed. We think that by dampening the seed for some days before sowing, the days to emergence after sowing would be reduced and predation reduced.
We would like to communicate with individuals who know about planting seed that have been treated for extended periods of time before seeding and individuals with suggestions about what we might do. We know that shading and moisture improve emergence. (3)
1 – Negreros-Castillo, P., I. Martinez-Salazar, C. Alvarez Aquino, A. Navarro Martinez, C.W. Mize. 2018. Survival and growth of Swietenia macrophylla seedlings from seeds sown into slash and burn fields in Quintana Roo, Mexico. Bois Et Forets Des Tropiques 336. 10 p.
2 – Negreros-Castillo, P., I. Martinez-Salazar, K.F. Kellner, C.W. Mize, R.K. Swihart, M.A. Navarro-Martinez. 2016. Bois et Forets des Tropiques 329. 10 p.
3 – Morris, M., P. Negreros-Castillo, and C. Mize. 2000. Sowing date, shade, and irrigation affect big-leaf mahogany (Swietenia macrophylla King). Forest Ecology and Management 132:173-181.
I need information about biostimulators for seed germination, plantlet growth and plant development of following medicinal plants:
1. Nigella sativa,
2. Trigonella foenum,
3. Coriandrum sativum,
4. Rhus coriaria.
Also, I would like to have some papers about development of stress tolerance of these plants both in vitro and in vivo conditions.
Hi colleagues, I have Arabidopsis thaliana wild type (Columbia) seeds. Seeds germinate well and their seedlings with first leaves (green) develop 1-2 cm long. The first leaves of seedlings are beautiful and green. However, seedlings do not develop after this stage and they remain very weak. Although I have tried many ways (Rockwool, hydroponics, soil, Petri dishes with Hoagland or MS), the seedlings do not grow and are the same even after 21 days.
I wonder what the problem is.
Thank you in advance for your contribution
I now try to writing research proposal about germination of seed from parasitic plant (in this case, i try to germinate the rafflesia seeds). If anyone here have some experiences in germination of parasitic plant seeds especially holoparasitic plant like rafflesia, i would like to get some advice and maybe technique to do it, and if already have protocol to do it, would you like to share with me.
thank you in advance. hope advice, comment and suggestion from all of you will help me to improve this research and i can start working with this project.
Suppose we are checking the effect of salinity on seed germination and we are measuring the effect by observing the lengths of shoot and root after a certain no. of days.
In order to see the salt concentration which gives the most significant difference between the control and the test samples, should we consider a non-parametric or a parametric test?
I want to germinate transgenic rice seeds (T2 generation) in hygromycin solution to see the segregation ratio of 7 events. Did anyone tried this before??
Will appreciate any comments on this.
I am performing seed germination study of fenugreek in controlled environment(lab).I want to use to germination chamber for the experiment. I want to know the temperature and relative humidity required for germination experiment of fenugreek.
I am looking for studies or key words that would help me explore the body of literature that tests whether seed germination is an inherently social or solitary activity in a plant species. Any advice or leads would be appreciated
I need these concentrations to prime my seeds for germination and emergence improvement.
we have done red cadamba seed germination in vitro by treating with fungicide and double sterilization with 50 % Clorox and denatured alcohol and inoculate it in MS media with hormone but it shows low germination rate even after a month .
please advice me on how to increase the germination rate and reduce the contamination rate
I found some grey/black spots on a filter paper when plant seeds were germinated. It is weird that the spots are not close to the seeds. They should not be derived from the deionized water because I set a control without seeds and spots did not appear there. What shuld be the reasons and how to deal with it? Thanks!
I am working on Okra tissue culture but i am facing problem about okra seed germination , for seed treatment i am using HgCl2 0.1% for 3 minutes and after washing with dd water use 75 % Ethanol for 15 seconds and washed with dd water for 5 times and soaked in water for one night and next day put in 1/2 MS media ( 15 g sucrose, 8 g agar and 2.37 g MS powder media for 1 litter ) and place in dark for 1 to 2 days until the plumes come out and then placed in florescent light . i have tried many times, i have changed seed from different companies but some time good germination and next time 0%. can anybody help me about this problem ?
This specie of family Arecaceae, known as vegetal ivory, is derived from a hardness of its seeds. Seeds germinate approximately 8 months after sowing.
In scientific resources is noted that the seed coat mucilage only physical barrier to seed germination (preventing the diffusion of oxygen).
Is it not possible to create a new chemical compound between oxygen and mucilage that inhibits of seed germination and embryo growth?
It's not possible?
hello every one,
Hope reading my question will all be in good health, can some one tell me why my seeds not germinate? actually i planted my alfalfa seeds on 12 June 2018 in sole cropping and inter-cropping with maize but the maize are now at 4-5 leaves stage and the alfalfa are not yet germinate, after two weeks then i reseeded alfalfa but again after three days did not notice any sign of germination could you please help me what are the key problems and their solutions.
I found from an literature that ft-1 mutant has reduced germination rate, while the insoluble tannin content in the seeds coat is high, so why is that?
Hello, can I get help on the rate of ethrel to use in peanuts to break seed dormancy, Is it the best way to soak or just spray the seeds prior sowing?. Is convenient to use ethrel only or it works well with other growth regulators?
I am working on propagation of Gmelina arborea ( Gamar/ sivan). The species is widely explored for agroforestry purposes but quality planting material is still a constraint. I not come across much seed and seed propagation studies in the species. Can anybody suggest/ provide some references/ article on Gmelina arborea seed and seed/ vegetative propagation studies.
We evaluated the response of tree seeds germination to application of something like "growth stimulator". Control, Low, Medium and High concentrations were selected. The thing is, that Low and High concentration of chemical significantly affected seed germination, while Medium concentration did not. Does anybody encountered similar behavior of some kind of chemicals/drugs/something?
Thanks a lot.
I am working with Festuca rubra and Agrostis capilaris growing in contaminated soils, but the seeds have very low germination rate. Currently I’m comparing germination and growth of this seeds vs healthy seeds. However it would be interesting to compare seeds vs clonal reproduction of the same genotype, but I haven’t found any paper a