Seed Germination - Science topic

Dear Aminu Moriki Ladan it depends of the number of parents involved in mating and their genotype. If either female and male parents are clones, F1 should be genetically uniform, at least external factors occur (mutations, contamination,...). However, if parents aren't clones, variations must be expected.

Visit kindly the following useful RG link:

Firstly, test seeds viability via tetrazalium.

The following links are also very useful: https://www.rimolgreenhouses.com/blog/pre-sprouting-seeds-101

Perhaps this article can be of your interest:

The answer to your question lies in the integrity of the cell wall. I just finished an experiment with one treatment having RWC > 100% and also high electrolyte leakage. In this case, FW > Sat weight and therefore, an RWC > 100%.

Terrestrial Orchids are well-suited for hydroponic growing, as they grow in moist, loose soil and need constant food and moisture to thrive, which is supplied with a hydroponic growing method. Selecting varieties suitable for the growing conditions in hydroponics improves chances of success.

Please see this.

Speed of germination= å x/N

i.e. X1/n1 + x2/n2 + x3/n3............. xn/Nn

Where, X = no. of normal seedling; N = no. of days

L. Marian Gonzàlez

This plant is either Albizia carbonaria (https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:8016-2) or Albizia julibrissin var. julibrissin (https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:77208306-1)

Thanks!

Agreed with Sajid Khan

Seed size and sometimes seed color can have a great variation in seed germination.

Thank you very much Elisa

Do not add zinc to soil since it inhibits seed germination :)

The question is very interesting

Coriander seeds usually atkes 2-3 weeks , depending upon quality . Most important is to ensure that they are not aged seeds and not for usage as spices...

Tetrazolium test very specifically mentions about the seed viability indications. depending upon the seed selected, your lab environment and tetrazolium test applied, you may like to develop your own protocol for your specific research

Description below may be useful:

i. Seed roll technique: Seeds may be rolled on 7-10 days old culture of fungi on the culture Petri plate, and Petri plate rotated in both clockwise and anticlockwise so that seed surface get covered by spore. The pathogen coated seeds are kept for germination on blotter paper, and then are kept in an incubator at 28- 30oC for about ten days.

ii. Seed soaking technique: This method is followed as paper towel technique. The fungus is cultured on Potato Dextrose Broth (PDB). Twenty ml of PDB is poured into 100 ml conical flasks and sterilized. The flasks are then inoculated and incubated for around ten days. The mycelial mat from the flask is removed and macerated in a warring blender along with distilled water for a minute. The inoculum is later collected in a beaker. In the meantime, seeds are immersed completely in the inoculum. These seeds are then placed side by side on a blotter paper (45cmx25cm with one fold) and are folded. The folded blotter papers are then placed in trays, and kept in an incubator at 28-30oC for around ten days.

For support: http://medcraveonline.com/APAR/APAR-06-00198.pdf

I do suggest the same mentioned by Do Bozena

Try with Agar 0.5% gel and a phosphate buffer pH 7.2.

GA is not a control; it is a treatment. You need to set up different concentrations of GA :)

Dear Paul Chandra Sekhar Chandra Sekhar Paul according to the ista "International Seed Testing Association" procedure distilled water is used for seed germination

I am interested , hope we will get some waiver for processing charges as MDPI are open access

i agree with Nitisha Srivastava

I think , this is the easiest method followed in all polyembryonic crops . Use of molecular markers is really difficult , where such mass scale production is required....

Zero tillage/Conservation agriculture

Dear Moaed,

If you are assessing the effect of soil on seed germination, so you should delicately dig and put the seed back quickly.

Do you mean MGT (Mean germination Time)?

Dear Ali,

I think this paper could guide you.

i had some experiment in salinity condition, probably in some pot, because of evaporation, the salinity deeply increased especially in light pot. also, in country we saw seed in the autumn( cold condition).

I agree with you, however cytotoxic analysis of certain nanoparticles on human cell line proved they're safe to be used, in addition some are effective in treating cancer cells

Temperature affects germination speed , but rate or percentage is affected by seed viability

Dear Noor,

Please look at this pdf:

Dear Noora,

It will decrease, may be by 50%. Soil characteristics is the main factor.

Sorry, no paper on comparative effect (pots/petri dish).

Regards

First, a simple seed germination test and be sure of the seed vigour and seed viability.

Thanks astawus

It depends on your seed species. Sometimes, cold is important to germination. Generally, seeds need cold temperature to reduce the effect of abscisic acid and enhance seed germination.

Dear Mr. Isam Al-madhagi,

Thank you for your answer. Our target is to obtain the plant from culturing the immature embryo. We can not extract only embryo from the seed that is the reason why we try to culture the half-seed. Our problem is that even we got the abnormal germination but after 3-4 times of subculture they will be brown and die.

You need to check for yourself :)

Please take a look at the following PDF attachments.

Anup Kumar Sarkar , you have pleinty of documents just make a research on google. Good luck

Dear Joel,

Thanks so much for your infromation!

Well dear Mohamad Issaoui , of course the need is to clone any of these plant species. However, in the starting question, there is no information as to WHY germinate seeds under in vitro conditions. To do what? They don’t germinate at all under in vivo conditions? Species rescue?

However, if ever it is to clone them, the worst thing that one can do is to germinate them. And that’s where the difference between Angiosperms and Gymnosperms come in the discussion. Any Angiosperm sp can do something after germination, what is not the case for Gymnosperms. And ontogeny has nothing to do in the story. It is a question of physiological age. Yes, physiology is the expression of genes through biochemistry, cytology, morphology, etc. Nothing new! Except for geneticists!

What is the point?

I think that Phenological studies in trees must be done every season by Foretsry department. The data could indicate several informations about climate change, impact of environmental conditions...

Freshly seeds can germinate easily. After a long time storage > 2 months, embryonic dormancy will settle and the seeds will be difficult to germinate.

25°C and you can go to 30°C for a fast germination.

Try only one minute in Sodium Hypochlorite at 0.5% followed by a goodd washing in sterilized water.

It depends on plant species. Just follow the links of María Beatriz Espinosa , ISTA gives a detailed procedure for each genus and species.

Regards

Dear Ashish, try to check the seeds viability using Tetrazolium.

t test if the data is normally distributed or chi squared test if it is not

Opuntia seeds imbibe water without disrupting the seed coat, therefore water soaking, although being an easy try, might not help by itself. I would try to remove a tiny fraction of the point of the seed (nail clipper might help) before puting them in a moistened substrate. You should also watch the temperature, it should not be too warm.

you can use the DMSO in higher concentration up to even 10% but it depend on your test.be careful about control test.

if your problem did not solve you can use DMF as mentioned above.

I appreciate your answers, please if you have chance read the explanation of the question.

From other you means which one ??

Abstracts:

1- Effect of diluted sea water on growth, yield, essential oil productivity and chemical composition of coriander (Coriandrum sativum L.) plants.

2- Efficiency of chemical, biological fertilizers and gibberellin on Coriandrum sativum L. under the condition of salinity and calcareous soil.

Apart from other environmental cues, its important to prevent exposure to relative humidity lower than 50% and lack of air exchange also prevent development of good leaves. We grown many of Arabidopsis accessions in small pots and prevented direct contact of leaves with the growing substrate using a black plastic sheet. When I grow them in rockwool or in vitro, the size of the leaf was considerably decreased. Have a look on the following paper:

Aliniaeifard S, van Meeteren U. 2014. Natural variation in stomatal response to closing stimuli among Arabidopsis thaliana accessions after exposure to low VPD as a tool to recognize the mechanism of disturbed stomatal functioning. Journal of Experimental Botany 65, 6529-6542.

see Daws, M.I., H.W. Pritchard, and J. Van Staden. 2008. Butenolide from plant-derived smoke functions as a strigolatone analogue: Evidence from parasitic weed germination. South African Journal of Botany 74:116-120

Not the plant holoparasitic family you are working with but may be of use

The following articles may be helpful for you.

Best...

Dear Hossein

thanks for the link, and comments, I am testing for homozygocity and no other analysis

To my mind, Temp. in the range of 25 to 35 degree is congenial for majority of the seeds to germinate since, metabolic activities of the cell is triggered at this temperature. humidity more than 70 % be beneficial. Personally suggesting variable temperature for day and night.

No tengo experiencias personales a su inquietud.No obstante pienso que la germinación de una semillgerminaciona va a estar determinada en en su vialidad,poder de germinación del lote y las condiciones agroclimaticas que perduren en la región que se siembren.Es importante proteger las semillas antes de sembrar con productos naturales o químicos para protegerlos del ataque de plagas y enfermedades

Dear Promise

I have sent three files.

You can use Excel file for calculating water potentials with PEG 8000 AND 6000.

Best regards

Farshid

No problem, you can do it. After priming it is better to grow the seeds in 1/2 MS media so this media will promote the root growth and you will have finaly more powerful plants. Good luck.

Hello

Are your seeds alive ? Which hormone and which fungicide are you using ? Why using MS medium rather gelled water or moisted filter paper ? Is there any seed dormancy for this tropical tree ?

Regards

MJ

Dear Mr.Zhou,

I deem the sterilization protocol too mild.

I seal the filter paper in a bag and autoclave it for 20' at 121°C reaching 300 kPa.

I shake the seeds for 8' in 400-1000 uL of a solution 12% sodium hypochlorite + Triton X-100 in proportion 1000:1, then centrifuge, remove the solution using 10 uL tip on a 1000 uL one in order to avoid taking the seeds.

Then I wash them with dH2O five times before spreading them on the filter paper with the last washing water and let them dry before use.

All done under under laminar air flow hood.

70% ethanol (1 min.) followed by 5% sodium hypochlorite (5 min.) and rinse several times with sterile water before sowing for germination

Dear Hafiz

In addition to the toxicity of mercury chloride, okra shows a particular kind of seed dormancy, called delayed permeability, caused by the structure of the seed coat and particularly by the chalazal plug. The combination of temperature and time (40°C for 4 days and 100% RH) were pointed out to solve the dormancy.

Nada, E., Lotito, S. and Quagliotti, L. (1994). SEED TREATMENTS AGAINST DORMANCY IN OKRA (ABELMOSCHUS ESCULENTUS (L.) MOENCH).. Acta Hortic. 362, 133-140

Dear Marcus,

Please check this PDF attachment.

Good luck!

Dear Maria:

Thank you for the answer

Intact seeds of Cistaceae , Malvaceae, Fabaceae and Convolvulaceae species generally have physical dormancy. The treatments such as mechanical scarification, acid scarification and dry/wet heat can be used to break physical dormancy.

For more details, see below articles.

Tannin content is known to be correlated with low seed germination. For example, see:

doi:10.2134/agronj1970.00021962006200060051x

Influence of Tannin Content on Preharvest seed Germination in Sorghum

Thank you all. The best application rate and application method in my experiment was 0.05%(w/v) ethrel and 0.625ml/l tebuconazole-carbendazim fungicide mixture. When sprayed directly into seeds in the furrow and immediately cover with soil, this displayed high efficiency compared to other methods I have tested. Once more I thank you all for taking time to help me out.

A study was attempted to evolve method for mass multiplication of mature ortets of G. arborea involving rooting of miniature bud sprouts.

This phenomenon is related to interactions of species and the concentration of growth regulator. In high or low concentrations, growth regulator effects may be inhibitors or stimulants.