Science topics: ObstetricsScreening
Science topic
Screening - Science topic
Screening, in medicine, is a strategy used in a population to detect a disease in individuals without signs or symptoms of that disease. Unlike what generally happens in medicine, screening tests are performed on persons without any clinical sign of disease.
Questions related to Screening
I am screening soybean genotypes for drought tolerance and vulnerability in a screen house. Some literature suggests 3 weeks and others suggest 4 weeks of drought imposition.
Is there a way to perform the blue white screening using plates without the IPTG? Can I replace it with another substance?
I'm carrying out a qualitative systematic review on the barriers to screening uptake. Following my search and screening for primary studies to be included, I ended up with 5 primary studies. 3 of these studies stated that they used quantitative cross-sectional study designs, however after reading these three studies, I have realized that they provide qualitative evidence, that is, they discuss experiences and barriers to screening which is relevant to my review. Given that my review is qualitative, can I use the qualitative data from these quantitative studies, provided I state this in my selection criteria?
I am planning to screen multiple GPCRs with different well-studied ligands. As a first check, I want to see if my GPCRs are expressing properly and are getting activated. Is there a quick and dirty way to test if it is doing what it is supposed to?
How can we actively reduce children's screen time in our tech-driven society to promote healthier habits and better well-being?
I need to use the AutoDock program, but I tried both versions 1.5.6 and 1.5.7. I had no problem loading, my only problem is that when I open the program, the loading remains around 9% in the window that opens. Then the window closes with the command page that opens. It will be more understandable when I add screen shots. I am using Win11 operating system, I have an HP brand laptop, I really can't figure out where the problem is. In the screenshot I attached, it stays as it is. The program closes directly.

Nowadays, we are encountering with screen addiction in children especially during lockdown. I want to know any guidelines for screen control?
Hello, I'm facing a conundrum and was hoping to get some advice.
We have 12 cryo tubes (all replicates of the same clone) of HEK293T cells preserved in our -80 that have supposedly had a plasmid cassette inserted into the AAVS1 safe harbor locus site (I can provide more info on this if needed). I say supposedly because we're trying to verify this using various means, including PCR.
One of the replicates is showing promise in our PCR screenings. It should match one of the 12 replicates in the freezer. The problem is, I don't know which one. The genomic DNA we've been doing PCR screening with was labeled with well numbers, while the preserved cells only have the cell count and the clone number (they're all clone 1). Obviously this was an oversight we're now paying for.
I'm discussing with my PI how to go about screening them via western blot, which would involve thawing them all out, taking a portion for whole cell extract and another portion to re-preserve.
The preserved tubes range in cell count from ~3 x 10^6 cells/mL to just 1.55 x 10^4 cells/mL. For the tubes with lower counts, can they still be thawed in a T25 or do I need to use something smaller?
I just don't see any other way to go about this other than thawing them all out, but if I'm missing something, I'm open to suggestions! Thanks!
whether journal editors are using any review AI tool for checking the paper quality for forwarding the decision to review committee or rejection of paper in first screening by the editorial team.
Hi I am doing a systematic review and completed my title and abstract screen in March 2024. After that I took some break and want to continue that work. Please guide
1. Do I need to remove all the previous work and start with fresh one?
2. Can I add up new searches for last one year by applying Filter?
3. Can I search again and merge the two(old one and new)?
Thanks
I want to study the effects of a pharmacological treatment (antidepressants) related to quality of life in oncologic patients. Apart from a depression diagnosis that would be a prerequisite for administering the treatment, i need another screening tool that could confirm the patient's ability to be functioning enough to give me true and valid answers later in the main tests. For this reason I am looking for a validated tool in clinical setting that could detect any cognitive impairment due to a psychiatric condition or substance induced (es. high doses of morphine).
I am currently conducting research for my master's dissertation, focusing on studying stress levels in poets. Therefore, I require a validated screening instrument to differentiate my target sample of poets from the general population. Please contact me, if there are any journals/research that you know of, that has this. Thankyou.
I can’t reset my
Password. Research Gate refuses them all. When it gives me a chance to reset them by pushing a button, the page won’t come up—only some letters to the left of the screen. Will you please fix this problem?
Ellie Ragland
I want to reset my password
I prepped a cDNA library from ovarian cancer cell line RNA (all RIN scores were 9+ on TapeStation 4200) using a KAPA HyperPrep mRNA kit and UDI adapters for Illumina sequencing. When we ran the TapeStation (D1000 HS screen tapes) on the prepped libraries, we saw extra peaks around 230-250bp. On some samples it was a more pronounced peak, but others it was more of a shoulder. We are baffled on what this could be from. We did a QC sequencing run on an illumina nextseq P2 100 cycle and are wondering if our inner distance plot could look like this due to the smaller fragments we see on the tape station? I've had some people suggest the extra peak on the tapestation a bubble peak from over amplification of the library, but bubble peaks would appear larger, not smaller. Our anticipated library size was 200-300bp. For most samples the average size was 350bp, so including the adapter sequences this seems right. We just have no idea what the smaller peak would be that appear in nearly every sample. I've included images of a couple electropherograms and gel images from the tapestation and the inner distance plot. You can see these smaller extra peaks appear as bands on the gel images and how they slightly vary in size from sample to sample.





The intersection of biometric screening and artificial intelligence (AI) is a rapidly evolving field, with significant potential for innovation and improvement. Biometric screening involves the use of physical or behavioral characteristics, such as fingerprints, facial recognition, or voice recognition, to identify individuals. AI, including machine learning (ML) and deep learning (DL), can enhance biometric screening by:
1. *Improving accuracy*: AI can analyze large datasets to improve the accuracy of biometric matching, reducing false positives and false negatives.
2. *Enhancing security*: AI-powered biometric screening can detect and prevent spoofing attacks, such as using fake fingerprints or faces.
3. *Increasing efficiency*: AI can automate the biometric screening process, reducing the need for human intervention and increasing throughput.
4. *Enabling multimodal biometrics*: AI can combine multiple biometric modalities, such as face and voice recognition, to create more robust and secure identification systems.
Machine learning and deep learning are key technologies driving the advancement of biometric screening. ML can be used to develop algorithms that learn from data and improve over time, while DL can be used to analyze complex patterns in biometric data. Some examples of AI-powered biometric screening include:
- *Facial recognition*: DL-based facial recognition systems can accurately identify individuals in real-time.
- *Voice recognition*: ML-based voice recognition systems can authenticate individuals based on their unique voice patterns.
- *Fingerprint recognition*: AI-powered fingerprint recognition systems can improve the accuracy and speed of fingerprint matching.
Overall, the intersection of biometric screening and AI has the potential to revolutionize the way we authenticate and identify individuals, with significant implications for security, convenience, and privacy.
This question explores the intersection of computer vision and AI with physical game design. It seeks insights into using these technologies to create interactive, screen-free games that improve children's engagement, cognitive development, and social interaction while reducing reliance on digital screens.
I need clarification on the following: If we use ASRIVIEW Lab tool for screening of Articles for conduting Systemtic Literature Review, should we mention the tool name in the manuscript?
Thanks
Hi all, I plant to creat a yeast mutant with two gene knockout. I want to use homologous recombination method. I found lots people will knock out one gene first, then continue with the second one. Could I knock out these two genes with kanMX and natMX at same time, and screen the positive colonies in media containg G418 and nourseothricin?Thanks.
Hi everyone,
During my lab work, I sometimes have to look for the existence of some specific X-nucleus containing components and for this, I perform X{1H} NMR screening experiments (e.g 31P{1H} or 15N{1H}...). However I noticed that ''sometimes'' I don't get any X nucleus signal while when I use 2D [1H-X] HSQC or HMBC experiments on the same sample I do get signals. Has anyone of you encountered this issue before ? could it be hardware a related problem, knowing that all the performance control tests on the spectrometer, gave good results ?. Also, the SW window is always set wide enough to look for any potential signal.
Thank you.
If we delay the arrival time of one of the two quantum entangled electrons from the monitoring screen, what will happen is that normally the two electrons must coincide (the arrival of the two electrons) at the same time to the monitoring screen. But what happened is that we delayed one of the two electrons, so what will happen is that it will happen, a contraction in the fabric of space-time so that the distance between the two electrons is equal. Therefore, the lagging electron passes through the contracting fabric of space-time between it and the monitoring screen, so that the distance between the two electrons is equal when they are observed. But because of that contraction, the delayed electron exceeds the speed of light and is quantum entangled with the other electron. Therefore, it will be in the past, and this indicates that if the electron is detected, it will affect the results of the experiment.
Dear colleagues,
We are conducting a systematic review and meta-analysis of cross-sectional data, and I am looking for a suitable workflow management software. Those that I have worked with (RevMan) or explored (Covidence) seem to be focusing on intervention studies that do require a comparator group. We don't have this in prevalence data. Does anyone know of a software that can do the job? If open source, even better. Note that I am not looking for data analysis packages, data analysis will be done in R. What I am looking for is a program to upload the references from the search results for title screening, abstract screening, full text screening and eventually, data extraction. Thereafter, we will export to R.
Thank you in advance for your help, best, Fabian
Zones which are greater use for quantitative screening. And don't know how to measure it's size.


We need to decrease the grain size of the mill scale in industrial scale to about 80mesh. for screening, which one is better vibration screen or rotary screens? also, about crushing machine which one is better, hammer crusher or ball mill?
Dear research community What will happen if we rotate the slit in terms of the pattern on the screen? Will it remain the same or will it change?
Thanks
P.S The picture must be misleading What was meant to be asked was a kind of pattern that the screen will give if the plane is rotating and not just stays in a steady position

Dear Colleagues,
I am trying to identify a pathway in cellular system and I need to do a kinase inhibitory screen. The one from selleckchem is very expensive. Do you have experience with working with inhibitor library from other companies? Or have you tried other methods to identify (kinase) pathways? Thank you in advance, #Kinase #inhibitory_screen #High_content_screening #pathway #inhibitory_screen
Dear colleagues
I have a GeneRead QIACube station which is specified for no longer supported Qiagen NGS GeneReader workflow (emulsion technology). It looks pretty much the same as casual QIACube, but has other worktable and screen. I just can't put a standard reagent tray into the device.
Is it possible to convert GeneRead QIACube (NGS sample preparation) into a QIACube for NA isolation?
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes (positively selected) but not the negatively selected ones. On the terminal, I get this error:
INFO  @ Mon, 29 Jul 2024 11:17:57:   Error in plot.window(...) : Logarithmic axis must have positive limits
INFO  @ Mon, 29 Jul 2024 11:17:57:   Calls: plotrankedvalues -> plot -> plot.default -> localWindow -> plot.window
INFO  @ Mon, 29 Jul 2024 11:17:57:   In addition: Warning message:
INFO  @ Mon, 29 Jul 2024 11:17:57:   In xy.coords(x, y, xlabel, ylabel, log) :
INFO  @ Mon, 29 Jul 2024 11:17:57:     1 y value <= 0 omitted from logarithmic plot
INFO  @ Mon, 29 Jul 2024 11:17:57:   Execution halted
I would be very thankful if someone with a similar experience could help solve this issue.
Thank you,

Hi, I'm going to use ReFinder to screen housekeeping genes, but I can't access to the Blooge Refinder or the (https://localhost/RefFinder-master/index.php) via XAMPP.
Does anyone has ReFinder access resourses that can be shared, please?
I am doing a gene editing experiment and I want to edit two sites at the same time, so there are two resistance genes, can I add two antibiotics to the cell after transfection 72 hours or do I add one antibiotic to the cell first and then add the other antibiotic to the screen? Is it possible that screening after two weeks of transfection has no effect?
Dear all,
We are producing activated carbon from hazelnut shells but have an issue about the dust of the a.c. granules also known as the dust film on granules. Linear vibrating seives doesn't do the job and granules are still dusty after seiving even multiple times. Dusty granules darken the water which is undesirable for water treatment applications. Can you please suggest any machine or a solution?
Thanks.
Covidence seems to have have very strong relevance in the medical and health sciences when doing a literature review. I am currently working on Students well-being in higher education using covidence for the texts screening. I have not been able to figure out the relevance of the quality assessment template in my data extraction stage.
I have tried to screen various phytochemicals of different plants leaves sample with antimicrobial property. I have tried aqueous extract of plants. But i have doubt whether sample is actually showing all of phytochemicals present in plant sample. How to screen whole of alkaloids, flavonoids, terpenoids or phenolics present in the plant sample ?
The protein was expressed using the insect cell system, and the recombinant plasmid was successfully constructed as verified by sequencing, and the recombinant plasmid was transformed into DH10 receptor cells for screening of blue and white spots, and the selection of white spots was verified by PCR reaction, and agarose electrophoresis showed that all of them were negative.
I am trying to find the Hansen solubility parameters for screening miscible substances. Could anyone suggest an open-source online tool to calculate the Hansen Solubility parameter or any other equivalent solubility parameters?
Dear scientists,
Faced now with the rediscovery of already known compounds, which natural source do you think would be best for screening and isolating new bioactive molecules?
Nice day!
We have two quant studio 3 real time pcr machines, no service contracts. One of the machines can run an experiment and the touch screen does work; however, none of the touch screen features work. This means we cannot open the drawer for the 96 well plate.
Has anyone had this issue or have troubleshooting experience with this?
I have conducted phytochemical screening followed by FTIR for an aqueous plant extract and I want to know how to interpret the FTIR results to determine which phytochemicals are present based on the functional groups that have been determined/predicted by FTIR. Is there any other way to interpret the results?
Hello I'm an engineer new to structural biology and helping to develop a cloud docking tool for screening compounds, similar to Swissdock but with mass throughput and GPU optimizations.
Specifically we're helping researchers repurpose existing drugs against protein structures simulated from the novel coronavirus genome.
I'm planning to use GPU optimized AutoDock-GPU , which takes in <protein>.maps.fld
I know you can use autogrid to select the bounding box and generate the .maps.fld, but I've been unable to figure out the workflow.
Also for preliminary screening I want to search the whole protein without specifying a bounding box.
Is there a script for converting protein.pdb to .maps.fld?
Thanks!
Professors, much like artists or musicians, wield chalk as a tool of expression and creativity in the classroom. In the act of lecturing, the chalk becomes their paintbrush or instrument, allowing them to dynamically articulate ideas and illustrate concepts on the canvas of the blackboard. The visual composition created through the arrangement of text, diagrams, and illustrations resembles the work of artists or the musical score of a symphony.
Lecturing with chalk is akin to a dynamic performance, where professors move fluidly, engaging students in a live and interactive manner. This performance aspect adds a sense of dynamism to the learning environment, much like a musician on stage captivating their audience. The tactile engagement with chalk further deepens the connection between the educator and the material, reminiscent of the intimate relationship between a musician and their instrument.
Professors, in their creative interpretation of subject matter on the chalkboard, make choices akin to artists interpreting a theme or musicians interpreting a score. The use of colors, emphasis on certain points, and the improvisational nature of the lecture contribute to a unique and creative interpretation of the content. The interaction with students during a lecture mirrors the engagement between musicians and their audience, creating a collaborative atmosphere that enhances the educational experience.
Beyond the practicality, the act of using chalk on a blackboard carries symbolic significance, connecting professors to a tradition deeply rooted in the history of education. This symbolic gesture aligns them with a legacy of knowledge dissemination, much like the choice of specific tools by artists for their symbolic value. In essence, professors wielding chalk as an artistic or musical tool enrich the teaching and learning experience, infusing it with creativity, expression, and a sense of tradition.
Chalk and blackboards have been a traditional and iconic combination in education for centuries. Some unique features that make them stand out as tools of trade, particularly for teachers and educators are:
- Versatility: Chalk is a versatile medium that can easily create both bold and fine lines. It allows for quick writing and drawing, making it suitable for a variety of purposes, from writing notes to illustrating diagrams.
- Interactivity: Blackboards provide a dynamic and interactive platform. Teachers can write, erase, and modify content in real-time during a lesson, engaging students actively in the learning process. This interactive aspect is crucial for classroom communication and participation.
- Cost-effectiveness: Chalk and blackboards are relatively inexpensive compared to modern digital alternatives. They are affordable for educational institutions, making them accessible in a wide range of settings, including schools with limited resources.
- Tactile Experience: The tactile experience of writing with chalk on a textured blackboard provides a sensory element to the learning process. Some educators believe that the physical act of writing helps reinforce memory and understanding.
- Visibility: The contrast between the white chalk and the dark blackboard background enhances visibility, making it easier for students to read and follow along. This clarity is important for effective communication in a classroom setting.
- Durability: Chalk and blackboards are durable and have a long lifespan when compared to some digital devices. They don't require electricity, and with proper care, blackboards can last for many years.
- No Technical Barriers: Unlike digital tools that may require technical expertise or face technical issues, using chalk and blackboards is straightforward. They don't rely on power sources, software updates, or other technical considerations.
- Nostalgia and Tradition: Chalkboards evoke a sense of nostalgia and tradition. Many people associate them with their school days and consider them an enduring symbol of education.
But subjectively speaking, professors, like artists and musicians, often develop a unique relationship with the tools of their trade, and for educators, chalk and blackboards hold a special place in their pedagogical approach. Here are some subjective perspectives on how professors might relate to these tools:
- Expressive Medium: Just like artists express themselves through various mediums, professors see chalk as a tool that allows them to convey their thoughts and ideas dynamically. The act of writing on a blackboard becomes a form of expression and communication, enabling them to illustrate concepts vividly.
- Creative Freedom: Professors may appreciate the creative freedom that comes with using chalk and blackboards. The ability to draw diagrams, sketch illustrations, and emphasize key points in real-time provides a level of spontaneity and creativity that can be challenging to replicate in a more structured digital environment.
- Personal Connection: Some educators feel a personal connection to the tactile experience of writing with chalk. The physicality of the process, the sound of chalk against the board, and the immediate feedback from students contribute to a unique teaching experience that fosters a personal connection between the professor and the material.
- Engagement and Presence: Just as musicians connect with their instruments, professors may feel a sense of engagement and presence when using chalk and blackboards. The ability to stand in front of the class, interact with the board, and gauge student reactions in real-time can enhance the overall teaching experience.
- Time-Honored Tradition: Professors may appreciate the time-honored tradition associated with chalk and blackboards. It can evoke a sense of continuity with the past, connecting them to generations of educators who have used the same tools to impart knowledge.
- Intimate Classroom Atmosphere: Some professors prefer the intimate atmosphere created by a traditional chalkboard setup. The physical proximity to the board and the ability to move around the classroom contribute to a more intimate and interactive teaching environment.
- Adaptability: Chalkboards allow for quick adaptability and improvisation during a lecture. Professors can easily erase and modify content based on student questions or the flow of the discussion, similar to how a musician might improvise during a performance.
- Symbolic Meaning: Chalk and blackboards can carry symbolic meaning for educators. The act of writing and erasing can represent the iterative process of learning, and the visible presence of the board serves as a constant reminder of the ongoing exchange of ideas in the classroom.
While technology continues to play a significant role in education, the subjective experiences and preferences of professors contribute to the enduring appeal of chalk and blackboards in many academic settings.

While performing STD screening I noticed that I have signals in my difference spectras, that are constant accross all samples, also if there is no ligand and only protein.
The protein was purified via size exclusion and is in deuterated PBS.
Hello
I m in need of protocol how to generate database in phase flr screening? The help from anyone is appreciated.
help me to cite pls. for our research purpose
I have the data on the DBH and the age of the trees. The farmers provided the ages, and some of them are not related to the reality of the DBH. Is there any scientific method that I can use to remove some ages that I can consider false data or outliers? Methodology: I can use DBH and ages to screen some data.
Thank you
I am conducting a research on mental health issues and help seeking behaviour of postpartum mothers with babies in Premature baby units and neonatal issues. I would like to know of a tool helpful to screen these mothers.
Dear colleagues,
A research team is conducting a study to evaluate emotions that have been automatically generated by using GANS, through a small survey. The survey presents 20 works of art in four different versions, each aimed at evoking one of the following emotions: amusement, delight, dread, and melancholy.
Your opinion is highly valued. Kindly access the form provided via the link and indicate the emotion you perceive each one of the 20 works of art to evoke. We suggest increasing the screen brightness to have a better view of the images.
Thank you for participating in this research. Your responses will be greatly appreciated. Feel free to share with your contacts.
I am trying to find any research related to mass health screening of general populations anywhere around the world.
Protocol for Screening Low-Density Polyethylene (LDPE)–Degrading Soil Fungi Isolated from Urban Waste Dumping Sites | SpringerLink
When aligning the objective aperture on a F30 in diffraction mode, does the electron beam need to be in the center of the screen? For example look at the picture I have shared. The beam is off centered wen in diffraction mode. Do I need to put the beam in the middle or is it okay if it's not?

Is there different solutions and new technologies or research on how we can help on this matter.
I have a highly conserved pocket in my enzyme that currently has no known function. I'm interested to see whether it binds a particular molecule. I'm wondering if anyone knows of an AI or software that can screen the pocket and identify molecules that are likely to bind there.
Thanks!
Hello researchers,
I am a student at the SOMT, university of Physical Therapy(The Netherlands) and I am studying for a master degree in Geriatric Physical therapy.
Currently I am working on a school assignment where I want to look further in differentiating sarcopenia (screening) in a nursing home. In a lot of literature I found that the Ishii screening tool is highly
recommended to use. Unfortunately I can't find this tool? This is maybe a bold question but can you maybe help me by telling me where I can find this exact tool?
It would really help me with my assignment :)
Thank you- anyway and also thank you for all your research and very interesting studies.
Is it more acceptable or tolerable of false positive or false negative in suicide screening for a suicide screening tool?
UNKNOWN DRUGS AND METABOLITES SCREENING USING LCMS
FROM SUSPECTED SAMPLES
I want to develop an understanding for conducting Meta-Analysis studies in Psychology. how many studies to select, how to screen them, any tools which are used for the purpose. kindly explain with references how to conduct a meta-analysis
I need a set of images of emotional faces (angry or sad; neutral; happy) of infants. I would like to frontally present these faces on a screen in a computer experiment. If infant images were matched in size, luminance, position, etc, with other images of adults, it would be great! Yhank you
My lab has the NEB Gibson Assembly kit (unfortunately not the Hifi kit) and I have been struggling for months to assemble various different constructs. I'm wondering if anyone has used this kit specifically and if there are any specific things/tricks you do or use?
I have tried to insert 3 fragments (ranging between 500 bp - 2 kb) into a vector backbone, as well as overlap PCRs to insert 1 fragment into the vector. All fragments have between 30-40 bp homology regions. I PCR amplify all of my pieces (including backbone) and gel extract them because we don't have DpnI. I can get the overlap PCR working well, so I thought the primers/homology regions were designed ok. Yet, I can't get a simple 1 insert into 1 vector reaction working - I consistently get zero colonies (occasionally I've gotten an absurdly low number like 2 or 4, but screening them they're false positives).
I've used NEB's positive control to make sure the Gibson mix is working and got many colonies, but unfortunately they don't provide much information on the contents other than it's 2 DNA fragments so I'm not sure how they designed the two fragments to be ligated.
I have typically been adding 50 ng vector and tried both equimolar amounts of insert and 2-3 fold molar excess of insert. Is there a concern that there is too much salt contamination carried over from the gel extraction? I was curious about the positive control NEB provides and nanodrop read quite comparable A260:A230 ratio between that and my fragment mix. I've also tried adding 5% DMSO into the reaction in case secondary structures were preventing efficient assembly, as well as transforming 50 uL of NEB10b with 2uL vs. 10uL of the Gibson reaction. And nothing seems to stick.
Sidenote I tried IVA once and didn't work for me, but if you have any specific recommendations with that I'd also be willing to try troubleshoot that method if I continue to have issues with Gibson.
According to an article entitled "Blood Transfusion Safety, current status and challenges in Nigeria" (2017), achieving blood transfusion safety in Nigeria (and likely the rest of Sub-Saharan Africa) remains a difficult task due to a number of factors, including a lack of blood, inadequate implementation of blood transfusion guidelines, insufficient infrastructure, and a high prevalence of transfusion-transmissible infections (TTIs), particularly hepatitis and human immunization. Even though there has been a slight improvement, particularly in the area of screening donor blood for common TTIs, significant efforts are still required in the form of effective public education campaigns (on blood donation) and ongoing system improvement to move the nation's current transfusion practices in the direction of safety and self-sustenance.
Source: Aneke JC, Okocha CE. Blood transfusion safety; current status and challenges in Nigeria. Asian J Transfus Sci. 2017 Jan-Jun;11(1):1-5. doi: 10.4103/0973-6247.200781. PMID: 28316432; PMCID: PMC5345273
Given the disproportionately high risk provided by blood-borne pathogens, regular monitoring of blood transfusions is required to avoid disease transmission. ELISA and Rapid diagnostic immunochromatographic technique are the two most extensively used methods for detecting HBV, HCV, and HIV infection in blood donors. Although ELISA are considered more accurate worldwide. With this, what are the drawbacks of using the rapid immunochromatographic kits for screening blood donors compared to ELISA?
Source: Al-Matary, A. M., & Al Gashaa, F. A. S. (2022, November). Comparison of different rapid screening tests and Elisa for HBV, HCV, and HIV among healthy blood donors and recipients at Jibla University Hospital yemen. Journal of medicine and life. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9762378/#:~:text=This%20study%20showed%20a%20significant,infectious%20markers%20for%20blood%20donors.
In responding to a request for a text I've always clicked on "View Request" and then get a screen where I can choose to respond or not. I don't get that anymore -- it's just an abstract of the paper they've requested. I can find no way to send the text. Please advise. Thanks.
I have try to establish a CRISPR screen in primary T cells,sgRNA transduce with
lentivirus and Cas9 protein delivered by electroporation (SLICE). But in the pilot experiment, we found that the efficiency of knocking out CD45 through this system is very low. The same experiment on Jurkat also failed.
Then I tried to change the experimental conditions, for example, increasing the amount of Cas9 protein or using Cas9 mRNA, or change CD45 sgRNAs.
None of these attempts have improved CD45 knockout efficiency.
I would appreciate it if you could give me some good advice!
Oral cancer has a high morbidity and mortality rate and clinical examination has resulted in late diagnosis. What reason has led to a delay in the implementation of a complementary screening test to the conventional clinical test?
covidence a screening software, and for full text screening if i want to keep article for my supporting document but not include in study how should i flag it?
Some papers refer to calculate FRAP value as
FRAP value = = [(A1 − A0)/(Ac − A0)] × 2, where Ac is the absorbance of the positive control, A1 is the absorbance of the sample, and A0 is the absorbance of the blank.
Some other papers refer to calculate FRAP value as
FRAP value will be Trolox equivalent using Y=mx+c equation.
Please let me know the original process for the FRAP value calculation.
Hello, everyone!
I am looking for a dataset where people recorded the simplest possible reaction time task. Something like pressing a button as soon as the screen turns red. Or maybe any variations that are not too far from such an experiment.
I know research on reaction time has been going on for the past ~40 years. But somehow I can not find a dataset for it. Wonder if any of you have seen it?
Thank you in advance!
Vitalii.
I need to screen the toxicity of several compounds that will be applied locally to an open wound of bone and muscle injury, which human cell lines (good for several passages) would be most appropriate for this experiment beside hMSC?
Thanks
I have already used the Capture SELEX method for the screen of small molecules.
I want to screen for antimicrobial activities of spices extract against fungal isolates from food. what method /methods is best for it?
For example QTL (x) is identified for disease screening in the mapping population where the parents are A and B. Can this QTL (x) be used as marker to screen the another population where its parents are C and D for the disease resistance?
E coli strain JM109(DE3) has its genotype listed as endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ Δ(lac-proAB) e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+) (λDE3).
It seems like it should be suitable for blue-white screening because of the lacZΔM15 deletion and but Promega states it isn't suitable. Another supplier, IntactGenomics says it is good for blue-white screening.
Anyone have an explanation? Is it just that it's an expression strain and not a cloning strain?
Thanks!
Antibiotic resistance , enzyme production are types of screening method . Both have advantages and disadvantages so , in these two which method is more preferred to insert in vector and provides maximum results . Are there any considerations of these methods while performing gene cloning?
I am conducting a systematic review and after screening have come up with 9 included studies 6 of which are systematic reviews. Am I able to use these in my report and discussion? As the articles the reviews utilise / mention are outside of our date range?
In the REDIAL project funded by Kidney research UK We are looking for a dynamic and talented early career researcher in the field of Computational Materials Science with a focus on Separation and Biomedical applications, who can effectively exploit Artificial Intelligence tools for the design of functional Materials and Sustainable Processes in the SusProM Group. https://www.suspromgroup.eng.ed.ac.uk/
The Opportunity:
- To work in a highly interdisciplinary Research Team with Chemical Engineers, Bioengineers an Experts in Artificial Intelligence and undertake research on the computational simulation and screening of materials for the miniaturisation and circularisation of the hemodialysis process.
- To exploit the research results to improve the life of kidney patients, in collaboration with Kidney Research UK and frequent contacts with patients, nephrologists and biologists during National events.
- To deal mostly with computational work, but willing to carry out experimental validation in the lab if needed.
Your skills and attributes for success:
- Computational material science skills (Molecular Simulations, Macroscopic models and Machine Learning)
- Strong fundamental knowledge of materials for separation theory
- Ability to apply fundamental skills to problem solving
- Coding skills (Python preferred, Pytorch/tensorflow desirable)
- Basic experience in the lab.
I wanna use it in TSA medium to screen some characteristics of bacteria. I used 0.25g PbCl2 with 0.3ml HCl 12M, but this did not dissolve PbCl2
Metabolomics has emerged as an invaluable tool for prognostic and diagnostic purposes, last in the cascade of others OMICS -genomics, transcriptomics, and proteomics. Omics training usually covers experiment design, data generation, and collection, data preparation, data analysis, and the last but not the least - data interpretation.
At the end of this meticulous energy, time, and financial-consuming path, it might be totally none sense to fail to put your results into the broader biological context.
For those like me that have never been trained to interpret metabolomics data, how can we make sure to not miss important points? Is Basic knowledge in Biochemistry, Physiology, or physiopathology of the disease of your interest, enough to harness the full potential of metabolomics technologies for biomarker screening u.a?
I would like to discuss with experts out there, the most important assets for a right and successful data interpretation of metabolomics data.
Thank you for sharing your experience in the Metabolomics journey as well.
I just established a cell line after long-term term culturing and would like to screen for contamination. I only have Hoechst 33342, and I would like to know whether it can be used for mycoplasma screening.
For example, if a biparental mapping population is screened for abiotic stress tolerance (drought/salt) with physiological parameters.
Hello everyone
I have done simulation of 100 ns by using gromacs.. and the following is hbond plot but its different i dont understand that every thing is fine but the data formate in .xvg file is different, its look like mixed up .. here i am attaching two screen shots but if you want to check the .xvg file then i will send you
Please someone help me i dont know the solution


Hello,
I'm looking to implement a fast method to screen anti-inflammatory products. Avoiding protein denaturation is linked to anti-inflammatory properties.
There is a nice number of papers describes a rapid and low cost screening method based on an incubation of the tests compound and a BSA solution (about 1 to 5% w:w) at higher temperature (usually 72°C) for a given time (between 5-20 min) buffered to ph6.4-6.6. Reading is done by spectrophotometry (usually 660nm). The method seems simple but when I try to implement, my reading is zero. I noticed that each laboratory introduces a slight change on the most cited protocol. I have introduced the major changes reported without any reading. Papers reporting the raw data indicates that after incubation the negative control (no test product of anti-inflammatory drug) DO is about 0.3-0.4.
Notice that all weightings, control and technicians have been investigated to try to find the root cause, unsuccessfully. Do anyone have any experience on this that can give me any advice?
Thanks
Are preoperative (3D printed) models, and drug testing/ screening models classified as medical devices?
What category of devices do they fall under?
Dear Colleagues,
I wish to perform a genotype-phenotype correlation study in a family with a known mutation in RPGR-ORF15.
However, the RPGR-ORF15 region is very tricky because it contains GC repeats and thus its not possible to amplify with standard PCR conditions.
Special protocols are needed for both PCR amplification as well as during Sanger sequencing.
Therefore, I would greatly appreciate if anyone could suggest me a commercial facility where I can send my samples for screening the specific mutation located in the RPGR-ORF15 region?
Number of samples to be screened = 05
Many thanks,
Atta
For example, when searching over the PubMed database, we get 125,000. But PubMed has some limitation that just allow us to screen until 1000 results. Do anyone come across this situation, and what you do to solve this issue, or anyway to report the results?
Hi everyone, I wanted to know if there is a method that allows us to screen bioactive molecules with protective power against UV rays. For example, exposing a support containing the molecules to UV rays, and then we observe directly (visually) a character that allows us to select the protective molecules. Thank you in advance
Field screening of rice genotypes against brown plant hopper was done on the basis of hopper population/hill/week. The genotypes are to be categorized under different levels of resistance but there is slight confusion in choosing the standard scale for BPH evaluation under field conditions. Please help verify the two scales attached in JPG format or share any article or web link regarding my query.
Thank you

I am working on the degradation capacity of naphthol by 6 bacteria isolated from wastewater which are : Pseudomonas aeruginosa , Pseudomonas Putida , pseudomonas fluorescens , Bacillus thuringiensis , Citrobacter freundii and Escherichia coli but I want to know which is the best composition of the minimum medium for the screening of these bacteria .
Thank you in advance.
Hi all,
I wonder whether there is a way to find the original natural product from the modified structure? It is very common to use natural product derivatives in HTS/HCS/in silicon screening to find more potent structures and/or SAR. Once, we have a candidate structure, is there any database where we can do search (based on structure similarity/substructure) and find the originated structure and the source (which plant, microorganism etc.)? Thanks
Best,
Burak
Transformed bacterial screeening
Hi everyone,
I am currently trying to screen the behavioral effects of the 6-OHDA unilateral injection in the striatum of Wistar male rats to confirm a Parkinson's animal model by apomorphine-induced turning behavior test (or rotameter test).
Although the dose of apomorphine is prepared according to the articles and is injected intraperitoneally to the rats, but instead of turning against the injection site of the neurotoxin, the rats become extremely relaxed and even fall asleep, and it is not possible for me to check the behavior.
I have request from dear students, professors and scientists that help me to find out what the main problem is and share the tips of rotameter test with me.
Please feel free to have contact with me.
Thank you for reading and helping!
I have National Cancer Institute screening results of my compounds. Upon submission, I am asked by the reviewers to provide standard deviations, what kind of statistical analysis was performed, and its significance. I have scourged the NCI methodology section and many publications throughout the years and couldn't find anyone who met these criteria.
I used 0.5% Aq. AcOH solution to dissolve chitosan and derivative to evaluate their antimicrobial activity and performed anti-bacterial activity and antifungal activity using the broth dilution technique in 96-well micro-trays (NCCLS, 1993) and the antifungal screening method (NCCLS M27-A2 protocol, respectively. The cytotoxicity tests of prepared chitosan derivatives were assessed by MTT assay.
Now reviewer have following comment.
kindly help me in replying the comments of reviewers
comment of reviewer: if the derivates are still not soluble in water how are they be used in the kind of treatment proposed? What it's the implication of using acidic media in antimicrobial or cytotoxic treatment ?
Hi all..
I am doing the in silico and in vitro anti-tuberculosis screening. Any one engaged in finding anti-tuberculosis compounds or related screening work. Hope someone engaged in the area can help me.
Thankyou in advance.
Shefin
I have a bit of a semantic question in relation to a paper I am writing.
Recently, I had a discussion with my supervisor on Type-II errors. In my recent statistic course, the type-II errror was defined as falsely not rejecting H0. A lack of power is often described as a cause of a type II error. As such, I was under the assumption that a type-II error is mostly used in the context of statistical tests.
However, my supervisor explained that "type-II error" could just as well be used describe any false negative result, also when it relates to methodological problems e.g. using the wrong measurement instrument for your aims and falsely not finding any effect.
I cannot find anything on pubmed on this matter. I screened some papers mentioning the Type-II error but all used it in a statistical context, not more general.
As such, my question is whether 'type-II error' only relates to a statistical problem or also to a broader definition of false negative outomes?
Thank you.
any technical input for PCR confirmation
I have read various articles in which plastic degraders are screened using PEG-containing MSM media. But which PEG is used is not mentioned. So, I used PEG-6000 as the sole Carbon source to isolate the plastic degraders.
I want to get suggestions about the best solid medium for plastic degraders.
I need to screen my plant extract for all the compounds present in it. So i want to know which technique (without using standards) will give me the results
I would like to create peptidomemetics based on a certain known peptide in silico.
For this I am asking if there can be any good references on this topic. (in silico specifically)
What I Want to do is; to create a small library of these peptidomemetics, then to screen their ability to dock a certain active site, then to run a molecular dynamics simulation to check the stability of the complex and the affinity to each of them.
Potential inquiries are;
Which software to use for building?
Which software for docking?
Which software to get parameters for these peptidomemetic to run the Mds? Or is there a preferred forcefield for these compounds?
Or
Should I deal with them in the same way as small molecules (this can be an answer that reliefs me at the end)
Is there any maximum number for studies that can be included in scoping review?
This is Homa Arshadi, Ph.D. Candidate of medical library and information sciences. I need some help with the following question. Could you please kindly advise me.
I'm doing a scoping review on text mining topic. The number of records identified after searching databases are about 10,000. After screening of 60 percent of these records, about 500 of papers are eligible, which must be read by full text. Is it reasonable to perform such review? What is the maximum number of studies to be included in a scoping review?
Many thanks in advance!
Kind Regards,
Homa