Science topics: GynaecologyScreening
Science topic

Screening - Science topic

Screening, in medicine, is a strategy used in a population to detect a disease in individuals without signs or symptoms of that disease. Unlike what generally happens in medicine, screening tests are performed on persons without any clinical sign of disease.
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Dear professors,
I am trying to do a spatial panel data analysis for Turkey Level 3 regions, but when I save the files from data.humdata.org in Stata, the error appears on the screen that the files are not compatible with Stata format. Stata does not accept these files with an out-of-format warning. Where do I need to upload these files in the program?
Kind regards
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Did you check what Stata says about this?
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We are researchers from University of Illinois. We are recruiting adults who have NOT used any crypto wallets (e.g., MetaMask) to evaluate the user experience of a crypto wallet. There is a $30 compensation! Please fill out this short screening survey: https://ischoolillinois.az1.qualtrics.com/jfe/form/SV_3t0IdsCLPRxfFlk
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Participation in research should be voluntary. Payment may influence the outcomes, impair your judgement and misrepresent the population.
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  • I am currently working on Mycobacterium diagnosis in bovines. I have tested clinical samples via conventional PCR screening with genus (16S rDNA gene) and MTB complex (IS0 1081 gene). I found the results that some of the samples were positive for the genus but negative for the MTB complex (having nine members including M. bovis, M. tuberculosis, M. orygis etc.) and vice versa. why it should happen that the genus primer is negative but MTB complex gives positive results? please explain.
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I Agree with Paul Rutland, you need to clarify your questions. It is conflicting
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Hey all, I'm an undergrad REU student at the University of Minnesota who's working on an independent project with the floral microbiome of Clarkia xantiana. I'm interested in the microbial diversity of the floral microbiome across a spatial range (i.e how different the floral microbiome of clarkia is from subpopulation to subpopulation) and whether a recently diverged subspecies of clarkia we're putting out in the field is picking up bacteria from the natural populations through some means (pollinators would be the most interesting, hybridization data is coming later so I might be able to see whether Clarkia xantiana ssp. parviflora we put out in the field are getting bacteria present on natural Clarkia xantiana after receiving natural Clarkia xantiana pollen and hybridizing). I've put this project together with the goal of characterizing the microbiome of flowers I have collected with the original workflow going something like this: Sample collection > sequencing > microbe species identification + measures of evenness and diversity of microbial species present on flowers. However, sequencing will not be possible by the end of the summer (I'd have to pool my samples with a grad student who is also doing sequencing after the summer is over to save money), and I'm looking at alternate methods of microbial characterization. One of these would be to develop a screening procedure with many different kinds of differential media, to determine what bacterial species are present in my samples. There are an almost infinite number of media to choose from, and without some preliminary sequencing data I'm a little lost as to what bacteria to even look for with my media. Acinetobacter would possibly be present (maybe Acinetobacter pollinis) and presence of saporophytes could indicate degradation or growth while samples were frozen in glycerine (failed preservation method). Staph or pseudomonas could also be a contaminant to screen for.
How might one go about devising a screening suite of differential media to inoculate with bacteria from my environmental samples? Should I say to hell with differential media, throw my samples in with the sequencing runs and be content with a smaller dataset to analyze at a later date (the 'success' of my experiment is not so important, except for my own personal satisfaction)? Or should I do more research into other methods of identification, like MALDI-TOF? Could really use the help!
Thanks,
Declan
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You need the selective media for Gram negative rods, Gram anaerobic rods, Gram positive rods, Gram positive cocci, Yeast and molds, Lactobacillus.
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I am planning a systematic review. For my last review, I searched different databases and uploaded the hits to my profile on covidence.org. However, their free trial now only allows 500 citations to be uploaded. I loved covidence's interface for very quick manual screening on titles, however, it's too expensive. Does anyone know of a free alternative, which is equally efficient for fast screening? Thanks in advance.
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Rayyan
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Q1. We have screened 120 cotton hybrids under two summer temperature regimes and recorded data for around 30 traits including morphological, physiological and quality traits, further, I want to study the behavioural changes of these traits which are ultimately responsible for the yield difference across two summer temperature regimes. Please help me to compare the two sets of means for 30 traits with suitable statistical analysis.
Q2. We also have data for 22 parents (Varieties) and their 120 hybrids, kindly suggest me a suitable statistical analysis for comparing parents and hybrids for 30 traits.
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This really sounds like one of those projects where your question should have been asked at the beginning of the year rather than after harvesting.
I'm guessing a simple t-test won't do, so you want some sort of multivariate analysis to deal with co-related variables (total leaf area is probably related to total leaf number for example).
But you haven't specified how many replicates you've taken for this experiment, or if you've only measured your variables once or repeatedly over the entire growing period. What is meant by "behavioural": during this year's growth only, or repeated years at the same conditions?
You might want to look at some sort of heritability analysis between parents and hybrids, with h2 and H2 calculations, but with only 120 offspring from 22 parents, that might be a stretch for even the most optimistic of statistical packages.
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I have basic idea of handling Vivado IDE but I need a resource from where I can understand those features which I generally don't use even it often comes on the screen. So please suggest for the same(other than Xilinx docs).
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Thanks Robert.
I didn’t mean to say that Xilinx docs have anything lacking. It meant for a compiled version of those documentation. Is there any ?
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screening for herbicide resistance and identify genomic regions associated with it in maize
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I am not expert in this field but I expect that even experts would need to know considerably more about your plans to be able to answer meaningfully.
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wanna check the group differences in several category variables using chi-square; the differences were all significant, which shall not be the case as to the data screening
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How would you define 'reliable' specifically for the issue at stake?
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Evaluation of PPV and NPV of a screening test
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With the numbers for PPV and NPV you show, the disease prevalence must but larger than 12.5% and it seems likely that it is much larger than that.
That does not seem like a screening test to me.
May I suggest that the question whether to call a test statistic high or moderate is not terribly relevant, but the the question should be: is it good to use this test compared to the alternative?
The alternative is perhaps a gold-standard test that you may not want to use because it is costly. Then the question can be parsed into: What are the disadvantages of a false negative and of a false positive result, respectively? And are those disadvantages small enough to decide to save costs by not using the gold-standard test?
The alternative may be something else than a gold-standard test but it still seems best to focus on the advantages and disadvantages (incl. costs, if you will) rather then whether to call something arbitrarily high or moderate.
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I want to rank my screened molecules based on the MM-GBSA and GLIDE scores.
But I am confused about which score is good -ve MM-GBSA score or the +ve MM-GBSA score. Please help me understand the MM-GBSA score better.
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MM-GBSA score is an aggregate score of the macromolecular generalized born and surface area solvation score. That is, it measures the amount of free energy involved in the particular interaction set (Protein-Ligand) in question. And obviously the higher the free energy, the better the interaction profile and stability of the complex. The more negative the number - the higher the free energy released in complex formation. So, if there are multiple sets of such numbers for different compounds in different poses. Arrange the set in ascending order - the most negative score is the best complex-forming set.
Glide Score shows the free Gibbs energy of the ligands as an aggregate of its own various poses.
Ideally, the rank the molecules - one should correlate the glide and mm-gbsa scores and find the most overlapping one for furthering studies.
Additionally, mm-pbsa score would be a better fit than the mm-gbsa score as that allows for a Poisson-Boltzmann surface area calculation.
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As stated, I would like to know what is the difference between NCBI blast and EzBioCloud?
I have sequences of multiple unknown bacteria and am trying to do the first identification on them using only the 16s sequence by sanger sequencing. Once they have been screened, I will pick the one that looks interested to move forward with the characterization.
However, when I tried to use these two services, I have gotten a bit different results.
For example, in NCBI blast, the sample I have generated more than 10 hits that have < 99% identity. However, in EzBioCloud, I can only get one hit up to 98.99% of similarity and it doesn't have a strain name or taxon name. The highest one with strain name and taxon name only has 97.91% similarity. Therefore, I don't know if this one can be considered a potential novel strain or if someone might already have isolated and identity it.
I was wondering what's the difference? Thank you.
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1. May I ask When I should click "type materials only" ? or should I do this every time I want to identify the sample?
-> If you want to know exact species name, should click “type materials only” but want to know distribution of similar strains do not click.
2. I just changed it and it turned out the best hit was at 98.66% similarity!! -> It’s on the border, however, this means need to confirm by genome indices such as ANI or AAI. Usually that value resulted new species.
3. I am also wondering how do I know if these sequences are misidentified? In the original result (without clicking the type materials only) the top 2 hits were new isolates from one paper that were published just last year. so I'm not sure how do I determine if this is reliable or not even when this sequence result was from one of the published papers? -> If top 2 hits have new name, need to think more but have previous species name but newly isolated strain, don’t mind it. Species is determined by comparison with type species.
4. In terms of EzBioClouds, how do you know it is an uncultured type? is it because it doesn't have a taxon name?-> Only NCBI accession numbers, its uncultured type.
Also circling back to Blast, the hit strain name of the first hit on EzbioClouds happen to be the first hit on Blast when I clicked "type materials only". Does this mean this one has been cultured but EzbioClouds has not updated its database? I quickly searched for the name of this first hit and it was only recently been proposed to a new species. So I guess that is why. But please let me know if anything I say doesn't make sense. Thank you!
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Religious and sociocultural implications of relating the discussions of HPV and pre-cancer screening by parents to adolescents and young girls in an African Setting
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Yes, parent can and they should. Although in the context of sub-sahara Africa, this is difficult because parents find it improper and hard to discuss sex with their children, as this is perceived to be encouraging them to engage in sex. However, with the new generation of parents and with more awareness and sensitization, as well as, inclusion of comprehensive sexuality education in the school curriculum, parents and adolescents are more likely to be more enlightened than in the past
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IDRS as an effective screening tool for diabetes at community level uses four parameters Age , physical activity , Abdominal circumference , Family history, with sensitivity of 95.12% and specificity of 28.95% in predicting the risk of diabetes with score more than 60.Are there are other community level screening tools in different countries . What are the parameters used in them?
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India is diabetic capital of world, with maximum number of diabetic patients. There is large burden of undetected diabetic cases in community. There is increasing risk of diabetes in urban slum, because of illiteracy, lack of awareness, low socioeconomic status and unhealthy life style. Madras Diabetes Research Foundation (MDRF) has developed Indian Diabetes Risk Score (IDRS) to detect undiagnosed Type 2 diabetes.
IDRS tool comprising of two modifiable (waist circumference, physical activity) and two non-modifiable risk factors (age, family history) for diabetes.
IDRS is a cost effective, simple, non-invasive and fairly accurate tool for screening of diabetes in the community.
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Most experts agree that adults should limit screen time to less than two hours per day outside of work-related activities. Excessive screen time not only produces its own negative effects like eye strain and headaches, but it also steals time from more healthful pursuits like forging real-world connections and exercise.
I usually spend 3-4 hours on screen besides work related activities and I am trying to reduce it.
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Dear Dr. Ghulam,
I am spending on phone, computer and tv about 3-4 hours daily, especially in the night.
With kind regards.
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I am part of a lab doing flow cytometry on blood samples from mice inflicted with TBI. Does anyone have a protocol for this? Also, any suggestions on markers/antigens to screen for?
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Dear Leroy,
In the paper cited below are some markers demonstrating the activation state of neutrophils. This is a study in human patients but you can easily adopt the protocol to mice. CD16 and CD18 are rapidly upregulated upon stimulation of the neutrophils. The systemic inflammation after TBI should be enough to induce the upregulation of those receptors.
It is Importen that work consequently unter cold conditions. The heparinized blond sample should be immediately coole down to 4 degree Celsius. Start the ytaining procedure within 20 minutes. Add 5 microliter antibody (for example cd16 fitc) to 50 microliter coole blood, mix carefully and incubate 20 min at 4 degrees in the dark. And 2 ml cold hypotonic erythrocyte lysing solution and incubate for Addition 10 min AT 4 degrees and in dark. Centrifuge 5 min AT 4 degree Celsius (1500 RPM with Standard Lab centrifuge, Rotor diameter 14 to 15 cm). Discard supernatant, wash once with cold PBS w/o Ca,Mg. Discard supefnatant and fix with 1 % Formaldehyde in PBS w/o. Wash once again with PBS w/o and resuspend the Pellet in 250 microliter PBS w/o and start measurement as soon as possible.
Best
Dirk
Holzer K, Konietzny P, Wilhelm K, Encke A, Henrich D. Phagocytosis by emigrated, intra-abdominal neutrophils is depressed during human secondary peritonitis. Eur Surg Res. 2002 Jul-Aug;34(4):275-84. doi: 10.1159/000063071. PMID: 12145553.
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Hi,
I am looking for someone who has done research using EEG and who would be interested in collaborating with me on a project about reading on screen. Do you conduct research using EEG? Are you looking for opportunities to collaborate?
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Turkan Ocal sure, let me know more about it.
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Hi everyone,
Later this year I will be conducting a repeated measures longitudinal experiment designed to quantify the long-term impact of Mixed Reality displays as user interfaces for viewing flight procedures/checlists on drone piloting performance.
Prior to testing, participants will receive training from instructors, and over the course of this training period, will execute 2 different drone flights (as shown in the image attached as 'Position Flight' and Traverse Flight').
Participants will be randomly assigned into 1 of 2 groups - the 'Hololens First' group, or the 'Screen First' group (an LCD screen is the control display condition). I will also be ensuring that an equal number of participants are assigned to each group.
Following the completion of the training flights, participants will perform three subsequent test flights:
  • Test Session 1 will involve participants executing 2 different flights not seen in training (shown in the image attached as 'Orbit Flight' and 'Recon Flight'), and will take place roughly 5 minutes after the completion of training.
  • Test Session 2 involves participants returning 10 days after Test Session 1 to conduct the same flights they executed in Test Session 1 (Orbit Flight and Recon Flight).
  • Test Session 3 involves participants returning 180 days after Test Session 1 to conduct the same flights they executed in Test Session 1 (Orbit Flight and Recon Flight).
My question, therefore, is do I need to randomise my participants into the Hololens First group or the Screen First group prior to each test (i.e. randomise participants before Test Session 1, randomise them again before Test Session 2, and finally, randomise before Test Session 3), or if it is okay to randomly assign participants to one of these groups prior to their training flights commence - with participants remaining in either the Hololens First Group or the Screen First Group for the entire duration of the experiment?
Thanks a lot in advance for your help - it is much appreciated!
Cian
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It looks like you want to quantify the long term effects of 'Hololens' and 'Screen' relative to each other, and not so much relative to no 'mixed reality display'.
In that case, I think that you need to cross over from 'Hololens' to 'Screen' or v.v. only once: after the third test run. Because if you cross-over after each test run, you'd measure the long term effects of both type of devices at the same time and cannot well contrast the results. And that implies that you can randomize only once.
Does that answer your question?
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I have designed the optimization experiment using Box-Behnken approach.
What should I do if any of the factors combination fails, for example because the aggregation occurs.
Should I review whole optimization or is there any method to skip the particular factors combination?
And if I need to review the whole experiment, what method should I use to evaluate boundary factors values? Screening methods I have seen require at least 6 factors to be screened.
Any help is appreciated.
Greetings.
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If particular factor combinations are leading to "loss of data" because you can not evaluate it, then you can set so-called constraints in your future DOE. As a result, you will work with D- (in screening) or I-Optimal (in optimization) Designs. You need these computer generated designs in your case, because the design is not orthogonal so classical factorial designs are not appropriate.
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My blot sometimes produces such results. Could someone perhaps explain why this is happening?
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restart the program
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Hi all,
I am currently doing a reaction where the reagent releases the Cl+ to generate an active species. So this liberated Cl+ is killing some of my starting material by doing the unwanted N-Chlorination. I was thinking of screening some potential halogen scavenger reagents & screened some of them (trimethoxybenezene, mesitylene, phenol, aniline, indole, pyrrole, etc.) but have still not been able to complete suppress this side reaction. But the data shows that it is working to some extent. So could you all advise some halogen+ scavenger reagents to screen further?
Thank you in advance.
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How about sodium bisulfite or sodium thiosulfate?
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It is my observation that the RG Score has encouraged members to contribute expert scientific answers on the discussion threads, because Answers can raise one's RG Score each week, while posting scientific research Articles carries less weight, because the non-discussion thread portion of one's RG Score depends quite heavily on the number of Reads and Recommendations which any posted article in a specialized field may receive. Time is also a factor, because the discussion threads are more readily accessible so that one can participate and contribute one's expert scientific opinions everyday and for as long as one is able, but researching and writing a scientific paper requires a significant amount of time, not to mention the wait-time during the submission, editorial screening, peer review, proofreading, press run and distribution processes.
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Well, Prof. Nancy Ann Watanabe, there were several ways how to increase scores in RG, but when the scores rating is going to be eliminated in July, then still there is a possibility to comment on papers and preprints & I guess in threads as well, But that those do not reflect more than a pure interacting work.
Rarely this is done on unique scientific criteria, it is a mixture of respect and happiness for other members works as well, recommendations and reading of articles and preprints will be left for those members, and editorials willing to share the material, and there will be a growth in the research interest score and the Citations and the Readings. Advisable in addition, to post preprints if it is allowed so by journals.
arXIv for example now has added now a DOI and the traditional bibcode that leads to NASA ADS. RG has a DOI but it is not indexed always. I guess questions and discussion will remain open and we will have private statistics on those. Mentions in RRSS such as Twitter also are now starting to appear, I got one, let us see if I get another one, they are more difficult to track than citations.
Projects are the other options, although I do not know if they increase any scores, they are more to showcase with nice figures and reference our studies and findings. They can be shared on LinkedIn. Following the same matter of projects, a button should be implemented by the RG AI platform asking us if we want to be added to a particular project by creators (researchers that we don't know, I apologize but this is not a "star trek borg community" to be conquered by any project, since we are added without concern and that leads to misunderstandings sometimes. So do not only score that is under evaluation, projects memberships inclusions should be part of a discusiion as well, even if that is a rule in RG, scores were also a rule.
We are living in very difficult times and we do not always seek opinions to be part of projects outside our fields or particular interest.
Summarizing:
  • Research Interest, citations, and h index will be publicity mentioned in our profiles with a modern statistics curve on the citations.
  • Mentions (Privately) and Readings (Externally) will be left to see as well.
Best Regards, thanks for asking. Hasta Luego.
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Hello! I am looking for a yeast-2-hybrid kit to buy for testing protein-protein interaction. By kit I mean something that comes with the vectors, the controls as well as the yeast strains. I have been using the LexA and ACT2.2 vectors and L40 yeast strain but since I'm facing some issues with the system, I was planning to buy a similar kit from a trusted source/company. I want to mention that I'm not doing library screens but just want to test protein-protein interactions between different proteins.
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takara or clontech is good (the matchmaker system)
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Zones which are greater use for quantitative screening. And don't know how to measure it's size.
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In addition, measuring the zone of inhibition could be trick. I have carried out some methods using ruler and you have to be careful your starting line.
If you measure across the zone, your answer should be divided by two.
Please not that the clear zone is only taken as the inhibition zone.
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We need your expertise & opinion!
Please fill in this questionnaire:
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Is best to prevention also to treatment.
The best test option for screening of CRC is FIT test and consecutively colonoscopy.
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Hi,
I am a newbie with Hybridoma fusion and selection via ELISA and would be greatful for some tips for a screening problem I have.
I completed the fusion successfully last week and there were colonies growing in a lot of the wells. I started the screening yesterday with the first 100 clones via ELISA for my target protein.
I have done this ELISA multiple times with mouse sera and it works fine- no background etc. But in screening my hybridoma supernatants i got a signal in all of the wells... I block with 5% FCS overnight at 4°C (minimum 16 hours). I guess I have a high concentration of antibodies from the supernatant binding the plate, but I don't know what would be the best option to stop this considering blocking is already quite long?
There were about 3 wells that were particularly positive in the ELISA and I decided to expand these in the hopes these were positive above the background of the plate. However, when i check these wells, there are clearly other adherent fusiform cells/ contamination? growing in these in all 3 wells (see attached pics). Can anyone suggest what this contamination is? and why it would give such a strong signal in the ELISA?
Thanks in advance
Hannah
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Thanks for the suggestions Nick.
There are no differences between the sera and supernatant assays, all antibodies/development is exactly the same. I wash with 0.05% Tween in PBS for at least 1 minute (soaking), 4 times between steps and 6 times between the addition of supernatant and the secondary. Pre immune sera and naive mouse sera is negative.
I have tried with just supernatant from myeloma cells and this well is negative so I am unsure whether it is an issue with the secondary/FCS. Neverthless I am trying a BSA block now and will increase washing before addition of substrate to see if things improve.
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Hi all, I am a PhD student trying to look at molecular changes in neuronal cultures using WB. We have had some issues in getting results and oftentimes we just get a grey screen with no bands at all. However, using the same antibodies and protocol other times we see the bands. We have changed the buffers, membrane type, protein samples, primary and secondary Ab, ECL, skim milk to BSA…and still sometimes we get just a grey screen. Does anyone have any idea?
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Hi, thanks for your answer, we can definitely try this today. But the antibodies bind to our protein because sometimes we see the grey screen plus the bands
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How many transformants have to be screened for expression to get a hit in Pichia pastoris (i.e to see a band on SDS page)
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Vanita Uppada Hi, actually I'm also trying to express my clone in PichiapPICZAplha A and B, in X-33) I am not getting even minimal expression. For transformation, I'have used the suggested method by Invitrogen and also the DTT and sodium Acetate method. I have plated transformed colonies on -600/ml zeocin for screening. Can anyone suggest what should I do? I'm still waiting for the answer it will be very helpful.
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working on find the new potent lead compound and to work on anti cancer activity
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Hello San, if you want to screen a particular linrary against the extracted feature to find the top lead compounds, MOE provides a wonderful toolkit to do that. I hope it will help at some point.
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I work with transgenic plants that we transform using agrobacterium. When calli produce tissues, I screen the tissues using PCR. Lately, I have been using thermofisher's Direct PCR. Through the past year, we have had no issues with the kit or with our positive controls.
I have been trying to screen plant shoots since April. These plants were transformed using agro with a PHA vector. There are five separate PHA vectors. For a positive control, we use the appropriate PHA plasmid (i.e. if the calli transformed with PHA 231 produces shoots and we are screening them, I would use the PHA 231 plasmid as the positive control). The plasmid stocks worked well the last time I used them, so confirm PHA vector insertion through a triparental mating protocol. My positive controls were bright and consistent in my agarose gels! This was in October 2021 and November 2021.
Since I have started screening plant shoots, the plasmids are not consistently producing bands. These are the same plasmid extractions used in October 2021 and November 2021. We have extracted new plasmids from E.coli plates and they are not working either. I have prepared reactions for separate PCR machines to determine if there is a problem with the machine. I have run other reactions (with different samples, primers, and positive controls) to see if there is a problem with the machine or my technique. These reactions work fine without contamination. The positive controls show up fine.
Sometimes, the PHA plasmids will produce positive bands. Sometimes, the same plasmid will not produce a band.. I don't know what the problem is.. Any suggestions would be greatly appreciated. I can provide more information or gel photos if needed.
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I agree with Paul Rutland. It sounds like you've ruled out everything EXCEPT the primers. It's not the thermalcycler, it's not the DNA extracts, it's not the PCR kit components....what else could it be? It would make sense to get inconsistent results if your primers have started to go downhill. One thing you could try is to double your primer concentration during PCR. If there are still active copies of primer, this might produce more reliable results OR at least give you evidence that primers are the issue.
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Project Title: Exploring the properties of the Accessible AQ in children.
Dear all,
I am currently recruiting participants to take part in my final year dissertation research project. I am investigating the properties of an accessible version of an autism screening tool (the Autism Quotient) when used with children.
The study has received ethical approval from Northumbria University (Ref:44763) and all data will be kept secure and anonymous.
We are looking for participants that:
  • • are aged 18 years or over;
  • • are able to give informed consent;
  • • have a child aged between 6 and 17;
  • Your child does not have to have a diagnosis of autism to take part.
The study will involve:
  • • Completing a few questions asking for demographic information about you and your child, such as age and gender.
  • • Completion of two questionnaires about your child. The first is a screening tool (the AQ) that measures autistic like traits, such as preferring set routines. These traits can be found among the general population to different degrees. The second is a screening tool for learning disability. These will take approximately 10 minutes to complete.
  • • Your child will be asked to complete two short questionnaires that are ‘easy read’ versions of the AQ.
If you have any further questions regarding the study, please contact sally.e.lamb@northumbria.ac.uk
To find out more information about the study and to take part, please go to: https://nupsych.qualtrics.com/jfe/form/SV_8zYLleeCRtFvKWG
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Dear Sally, the offered link is not available. Regardless, I wish you all the best during your research!
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I know I am looking for an MOI of 0.3, but I am trying to figure out how one can ensure this.
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this is not in my field of knowledge.
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A 27 year-old married woman was admitted due to pain in lower abdomen for 7 days. USG revealed a multilobbed and multiloculated cyst in the left lower quadrant. During laparotomy, the parietal peritoneum was found thick. It was opened. A cystic lesion containing straw colored fluid was found. loculi were broken. Biopsy was taken. She had amenorrhoea for last one year. 
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I am trying to screen HEK293T cells by immunofluorescence and even though I coat the 96 well plates with poly-l-lysine (pll)after multipe washes (being very careful) there are few cells left. Has anyone fround anything better than pll?
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Malcolm Nobre thanks for your reply. I have no problem with cell detachement when culturing cellsthe cells detach after fixation and many washes during immunofluorescent staining (even when really careful. I am going to try Cellbind plates and poly-D-lysine. thanks again
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Objective: Identify the best metal complex and reaction condition for getting the target product with maximum yield.
Question: While optimizing the reaction conditions for a particular reaction, catalyzed by synthesized transition metal complex, what kind of order would be better?
a. Fix a metal complex and screen all other conditions initially (like solvent, oxidant, time, etc.,). Then finally screen the 10 different metal complexes?
(or)
b. Fix all the reagents (provided the reaction is happening) and screen all the metal complexes first. Then screen the different reagents?
Note: The no. of synthesized complexes = 10
With a model reaction condition based on the literature, the catalytic activity of the first complex is confirmed.
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Hello Premkumar,
thank you for posting this very interesting technical question on RG. We are synthetic inorganic chemists, so that I'm not really a specist in catalysis. However, I know that the investigation of catalyzed reactions often involves trial and error, and to the best of my knowledge there is no general rule on how to proceed. For example, when you say "Fix a metal complex initially and screen all other conditions (like solvent, oxidant, time, etc.,)" it is the question: How much time do you have? Can you afford to spend weeks or months varying all the reaction conditions?
On the other hand, when you ask "Fix all the reagents initially (provided the reaction is happening) and then screen the metal complexes?" my questions would be: How many metal complexes do you have available in your lab for screening? Can you afford buying very expensive rhodium or platinum complexes?
In general, I would strongly suggest to first read a review article about your envisaged reaction and do a literature search first to find out which metal complexes and reaction conditions are suitable in your case.
Good luck with your work!
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Hi,
I was working on a thesis project on a portable ECG device, I checked these
So it was basically biopotential->Amplifier->logging->displaying using Teensy(Programmable chip), Adafruit(Bluetooth Module), SPI LCD Screen, and resistors and capacitors.
I developed and assembled all except Teensy, adafruit, SPI LCD screen, as I don't have circuit on how to connect output to these 3 devices.
Can anyone share some other resources it would be very much valuable.
Thank You
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Dear sir, I guess the Teensy is used to acquire the signal (use one of the analog inputs; for instance, pins for 14 to 23 Analog). The signal is of course converted (Analog to Digital) inside the Teensy. Then, you can display what you read using SPI (pins 10 to 13 of Teens) on SPI LCD Screen. For the Bluetooth device, I don't know what is it used for; maybe to save the data on an SD card and send the data (acquired ECG) to a remote station (computer I guess). You can use it. It is not difficult to assemble that and programs exist for each task: 1. Acquire analog signal using Teensy; 2. Display a value, figure using SPI of Teensy on an SPI LDC display; 3. Send data using a Bluetooth device.
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Hi all,
I'm working on docking with Autodock Vina in Chimera. When I try to reopen a session I saved with .py or .pyc extensions, the type selection screen is opened for the designation of file type.
Which file type should I choose? Or is there something I need to do to avoid this screen?
I really appreciate your interest and help.
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I don't know about MOL2 but PDB will save everything for you (except for colorings).
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I need to device an experiment with the n-Back Pointing task without any hardware complement; e.g., no tablet to execute the reaching movements or computer touch screen to select the presumed correct items. The paradigm should be consistent with an ambulatory setting, as a traditional neuropsychological test.
Thanks in advance.
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I need to device an experiment with the n-Back Pointing task without any hardware complement; e.g., no tablet to execute the reaching movements or ...
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I performed a study where i screened for a certain rare disease in an animal population. The number of animals screened was 503, and the test I used had a sensitivity of 99%. I was asked, what disease prevalence my study could detect given the sensitivity of this test and the number of animals i have screened. I am struggling with this question, and appreciate if anyone can help with it. Thanks!
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rare disease should be screening from a high-risk population.prevelance is more among high risk people .so if 503 animal from high risk group, prevalence is more . if we don't do screening from all group of population , sample size should be more
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Dear community of researchers,
I am currently working on a small research project that will explore community- and patient-led strategies for increasing referral of diabetes and hypertension and raising awareness of these two diseases in Mozambique, a highly resource-constrained country.
I would like to ask:
- does anyone have knowledge on patient-led referral strategies and advocacy activities? If so, could you please share any relevant links and/or are you aware of any recommendations on this from international health organisations?
- do you believe that involving patients in such activities would be ethically appropriate? Why/ why not?
Thank you in advance for any replies.
Regards,
Chiara
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Thank you very much Jehan for sharing your perspective, that is very helpful.
To be more clear, with "referral" I referred to the identification by T2D patients of individuals with risk factors for T2D, such as being overweight and having excessive thirst or urination, and their referral to healthcare professionals.
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I myself have experience only with: https://www.rayyan.ai/
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You'll find a wide range of tools to carry out a systematic review and meta-analysis.
For the screening phase particularly, I've used abstrackr (http://abstrackr.cebm.brown.edu/account/login), revtools (https://revtools.net/), and screenatron (https://sr-accelerator.com/#/screenatron), all highly recommended!
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I will be grateful if you could share with me the screening tool that you use in your diabetes clinic to screen for any mental health problems among children and young people live with T1DM.
Thanking you in advance
All the best
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I recommend that you read the recent publication by Duffus et al. Published in Diebetes Spectrum in 2019:
"Mental Health and Behavioral Screening in Pediatric Type 1 Diabetes"
Diabetes Spectr. 2019; 32(2):171–175.
Good luck!
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I am doing research (Master Thesis) on a new topic, which is: “Blockchain, Accounting, and Auditing”. Where the topic will be addressed in its broad form and does not specialize in a specific part only. And I found that the previous research is more than 100 research. Do I show it in its entirety in the Literature Review or are there certain conditions for screening?
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Skimming is the best solution!
Just go through the titles of all the literatures available and find out the related articles that matches your title &/ objectives.
I hope that the answer will be helpful for you.
Regards,
Dr. Mahmuda
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How many chances of acceptance of a Manuscript, if editor send it our for peer review after preliminary screening?
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I would add that there' a lot of variation from one journal to another. Much better in some, less good in some others. The acceptance rate is often shown on the website of the journal, after their IF... some journals show their so-called "quality" by showing they have such a strict policy and a high rejection rate.
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I did a qualitative screening of 2 plants extracts. And both plants showed a negative result for polyphenol but positive for flavonoids. I know flavonoids are a class of polyphenols so I'm a bit confused on how to interpret that. And take into account that the quantitative screening for TPC (total phenolic content) shows results.
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Hi everyone,
My supervisors have asked me to seek advice from external experts in the field of human factors, augmented reality, psychology etc. to see if the wording of my hypotheses makes sense prior to commencing data collection in the coming weeks.
In my experiment, I will be training participants to execute drone flights (using the National Institute of Standards and Technology (NIST) Open Test Lane) while viewing flight procedures on a nearby screen (Condition 1), on a Microsoft Hololens 2 (Condition 2), and having them read aloud by a non-flying co-pilot while wearing an EPSON Moverio BT-300 headset (Condition 3).
On Training/Testing Day 1, participants will perform a series of training flights to familiarise themselves with the drone controls, and will also acquaint themselves with operating a drone under each of the three conditions mentioned above. Immediately following the completion of training, they will execute three different drone flights (1 flight per condition) and their baseline performance recorded.
This is a repeated-measures, within-subject experiment design, so participants will execute the same flight procedures (in a randomised order to facilitate counterbalancing and eliminate any learning/practice effect) under the exact same conditions as the test flights on Testing Day 1.
We will be measuring participants total elapsed flight time, as well as the number of errors they make on each flight. Thus, we expect that participants will execute the drone flights significantly faster and with significantly fewer errors while using the Moverio compared to when they use Hololens and the digital screen.
In addition to this, participants will return 4 days later to perform the same flights, and one final time 6 months later which will allow us to see how participant performance compares over time across all three conditions.
Therefore, my core hypotheses are as follows:
1. We hypothesise that using a heads-up, head-worn AR display (i.e. EPSON Moverio) to view drone flight procedures would result in better long-term retention of a newly acquired motor skill than using a head-worn AR display, or an LCD screen.
2. We hypothesise that using a heads-up, head-worn AR display (i.e. EPSON Moverio) to view drone flight procedures following a training-performance interval of approximately 6 months would result in participants making significantly fewer errors in the execution of a newly acquired motor skill than using a head-worn AR display, or an LCD screen.
I am attaching a series of images visualising and hopefully clarifying all of what I have said above. However, if you have any comments or queries of your own please make them know here in your responses and I will be happy to share more information.
Thank you all in advance for taking the time to help me out, it's very much appreciated.
Kind regards,
Cian
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Thank you very much for your advice Béatrice Marianne Ewalds-Kvist - your insights are very appreciated! Thanks for taking the time to help us out.
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COVID-19 is mainly a respiratory disease that affects the lung, although other organ structures with endothelium seems to be affected too.
When should we do imaging?
What is the aim of the imaging?
How can it help with management?
Do you agree with the following consensus statement?
How will you adjust your own practice and difficulties encountered? Why?
Ref:
The Role of Chest Imaging in Patient Management during the COVID-19 Pandemic: A Multinational Consensus Statement from the Fleischner Society. Chest. 2020 Apr 07.
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I personally believe that imaging examinations in covid should be rapid, simple and executable at the patient's bedside and therefore I believe that the most useful is LUS.
Unfortunately still today is used the chest X-ray that has been proven useless.
The purpose of LUS is to stage the pathology in order to predict its evolution, unfavorable or favorable. With LUS and blood gas analysis we can determine which patients should be discharged at home in a period when bed meals are scarce in all hospitals.
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I am currenctly trying to crystalize a protein with the His tag. My protein has precipitated after buffer exchange but my supervisor said it is ok. After a screen gave us some hits, I tried to range the pH and the PEG concentration in a new 24-well plate. Some drops look very oily-like, like it has been liquid-liquid phase separaration, while others seem to have clusters/aggregates of the protein. The thing is that I took protein precipitate from the Eppendorf in all cases. Anyone has any idea what happens or how I can resolve this?
#crystallography #drop #screening
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First of all, I would not try to crystalize an aggregated protein. Is this aggregation reversible? BTW, what explanation your supervisor gave to convince you about using precipitated protein in a crystallographic assay?
The best would be to find a buffer/pH condition where your protein is soluble. Maybe adding 5-10% glycerol to your buffer can help.
Moreover, from my experience, His-tagged proteins can aggregate more easily than the proteins without tag.
Don't worry, oily drops can be pretty common.
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Good day we have a serological ELISA test we commonly use for screening herds for a viral infection with an established cutoff for use in individual animals. We wish to investigate the AUC of the test when we mix equal volumes of 1 positive test and 2 negatives (pool of 3) and 1 positive and 4 negatives (pool of 5). I am willing to recalculate the sample size after the initial attempt and add more if necessary. Just wondered what the equation is for a non-pooled AUC and then pooled if there is one? R package if there is one?
Thank you.
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Why W = 0.1?
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Hey,
Your computer was infected with my malware, RAT (Remote Administration Tool), your browser wasn't updated / patched, in such case it's enough to just visit some website where my iframe is placed to get automatically infected, if you want to find out more - Google: "Drive-by exploit".
My malware gave me full access and control over your computer, meaning, I got access to all your accounts (see password above) and I can see everything on your screen, turn on your camera or microphone and you won't even notice about it. I collected all your private data and I RECORDED YOU (through your webcam) SATISFYING YOURSELF! After that I removed my malware to not leave any traces. I can send the video to all your contacts, post it on social network, publish it on the whole web, including the darknet, where the sick people are, I can publish all I found on your computer everywhere! Only you can prevent me from doing this and only I can help you out in this situation. Transfer exactly 500$ with the current bitcoin (BTC) price to my bitcoin address. It's a very good offer, compared to all that horrible shit that will happen if I publish everything!
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Definitely malware
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Hi all,
I am doing research on varietal screening of rice germplasm against brown plant hopper and white-backed plant hopper under field condition. According to IRRI standard evaluation system for rice, the screening of rice germplam against these pests based on its symptom i.e. hopper burn. Therefore, my question is how can differentiate the symptom "Hooper burn" whether it is caused by BPH or WBPH.
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BPH infested field = Circular patch is seen at severe infestation
WBPH infested field = Patch observed in the field is Spindle shaped
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For doing LCMS analysis of metabolites from crude samples of seeds, what is the procedure for fixing internal standards? Do we do multiple screenings until we get a detectable concentration peak of the standard and then use that for further analysis?
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Please, cosider, also the following attachments, associated with reference [1], shown in my preceding posting:
(i) MTZ-urine-equ.tiff: It contains our innovative basic equations (1) and (2);
equation (3) is according to Arrhenius's theory;
and
(ii) MTZ-urine-dqc.tiff: It contains diffusion coefficients of MTZ in urine according to reference [1] and equations (2) and (3). As can be seen, there is excellent correlation between our theory and Arrenius's one.
Once, when there are assigned MS ions to corresponding 3D molecular structures, there is not needed employment in internal standard.
There is, in actual fact, excellent-to-exact liner correlation between two completely independent theories: Our stochastic dynamic formulas, mentioned above, and the Arrhenius's one.
Thus, 172.072 belongs to N3+-cation of MTZ, while MS peak at m/z 172.041 is assigned to N+O-cation. MS peak at m/z 171 belongs to cation-radical of MTZ.
The shown equations describe completely and absolutely mass spectrometric phenomena toward (a) quantitative and (b) 3D structural analyses of analytes.
In other words, even in complex matrix like urine, there is completely able not only to determine analyte quantitatively, but also to determine its 3D molecular structure; furthermore, exactly in chemometric terms.
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For my current research project, a rapid scoping review, I am looking at different software tools aiding the systematic review process. After having read a systematic review on the topic: and some smaller reviews by librarians I am not really sure if the effort to set it up will bring any benefit to the review process and the research. In this project I predict easily more than 50 full-text screens, hence I would really appreciate some real life user experiences and opinions.
with kind regards
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Hi fellow pioneers,
I was wondering if there is a good strategy for designing a set of experiments to find the factors with the most effect on the response, which is nominal (yes/no, pass/fail type of response) instead of the typical continuous response? While for nominal response a logistic regression can be performed on available data, I doubt the usual factorial/fractional factorial design still works in this case (since they are meant for continuous response). What would be a suitable approach in this case? Kindly point me to any relevant terms/theories if anything comes to mind.
Thanks in advance.
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Wrong. Factorial design will work here if I understand your description correctly. See Montgomery Design and Analysis of Experiments available in the z-library for download. Thinking in terms of regression rather than ANOVA should be of help to you. Best wishes David Booth PS follow up with Response surface methods to see if you can optimize your design.
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I am trying to obtain crystals of proteins and after screening small individual aggregated precipitates appear in different conditions, does anyone know how to optimize further.
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iCristallization is not so rational, you need a lot of luck to obtain good cristals and sometimes the results are not prefictable
However is difficult to give you any suggestion if we do not know the conditions that you have already test:
I suppose that you already tested:
- different protein concentrations
- a large panel of screening buffer
- different temperature
- did you check in some way the folding of your protein?
eg, NMR, DSF, functional assay?
- do you know if your protein may bind some cofaction that may stabilize it?
- do you know if your protein at the C.- or N-term may contain some unstrctured regions?
best regards
MAnuele
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Dear all, could you please explain to me the reason why it's not possible to screen via western blotting?
Thank you!
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Mario Romani Denis Komarov Thank you Dr Mario and Dr Denis for clarifying my doubts!
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I face the following error:
Undefined function 'Screen' for input arguments of type 'char'. Error in IB_BaselineAction (line 29) Screen('Preference', 'VisualDebuglevel', 0);
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% initializing PTB screen
Screen('Preference', 'VisualDebuglevel', 0);
[won, rect_window]=Screen('OpenWindow',0, colourbackground, ScreenSizeInPixels);
flipInterval=Screen('GetFlipInterval', won);
HideCursor;
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I am screening soybean genotypes for drought tolerance and vulnerability in a screen house. Some literature suggests 3 weeks and others suggest 4 weeks of drought imposition.
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Hello,
I can play video using PTB on MATLAB. However, as you can see by Simplemoviedemo, the movie frame is quite small. How can I play it on the whole screen?
I would appreciate for you help.
Thanks
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In our daily drug analysis. We note sometimes the screening results are higher than the confirmation. For example, THC is in most time the screening concentration is higher than the confirmation.
Any answer or comment is really appreciated.
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Depends on what test you are using. In my opinion quantifying urinary THC (or carboxy THC) concentrations provides no clinically useful information. Virtually all POC tests just say positive or negative. Because THC is sequestered in fatty tissue and can be present for a long time, it certainly does not tell you how much, nor when the cannabis was smoked/ingested.
Is this important for research purposes?
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Currently, I am studying the working on the OLED screens in detail. I have come across various layers of systems, for example, HIL, HTL, ETL, EIL and EML. I have studied that each layer has energy difference in it. Why that is? is it necessary? why can't we directly connect the Emissive layer to the Anode and Cathode? what is the role of the energy barrier in it? I am adding one image with this question.
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Dear Prerna,
Indeed one of the first OLEDs was an anthracene crystal connected to an anode and cathode. However, these simple OLEDs were not very efficient, due to the low EL and light extraction efficiency. Therefore, muiltilayer OLEDs were invented to improve the efficiency of radiative recombination of charge carriers. This also implies the use of aligned electronic states to foster the charge carrier flow. Appended you will find a chapter of my lecture, which might help a bit.
All the best,
Thomas
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Hello,
Basically just what the questions asks. Are there any programs developed for this aspect? I will have individuals screening/eliminating articles and I was curious what the best fashion would be to extract them and then to screen for abstract/full text where everyone could access the files. I used Zotero in the past but this next one I am to embark upon will have a much steeper load in terms of articles extracted. Thank you for your time!
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I used Covidence for my last scoping review and it enables a very structured methodology and it's also very helpfull for team process. Good luck with your work :)
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*Question for psychtoolbox users*
I using one screen. The function "flip" flips all screen. Can i divided the screen to two areas of "flip"? I have two stimuli with different time display flipping one of them flips both of them. Any suggestions how to solve this?
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I am looking for information self-administered cognitive screening test that have been used for online studies without direct contact with participants due to the need for completely anonymised data (meaning, no interviews required).
I'd be very grateful for your help!
Lucas
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Just general cognitive decline, I would need this general screening as an admission procedure for participants in a study. Older adults without cognitive impairments would be the target population.
I could not find anything used in online studies, just some that could be adapted to be used in such way, but have not been validated (SIS, AMT, 6CIT).
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Is it possible to obtain a value different to zero when we evaluate a phenolic content in a plant extract which phytochemical screening didn't show it? If yes, what can be the reason for that ?
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Yes it is possible. In addition to Floyd answer above, recall that phytochemical sreening is a preliminary and not a confirmatory test. There is possibility of false positive and false negative result. Again there are different classes of phenolic compounds, one or two of them may not sensitive to your phytochemical screening depending on your test reagent. That is why we have more than five reagent to test for flavonoids for example due to different classes of flavonoids. Also, you have to prepare your phytpchemical screening sample well to allow you detect colour change and ppt present even at small concentration of the phenolic constituents. Try and use control when performing the test so that you can detect minor change in colour by comparism.
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The sample was subjected to centrifugatiin at 9500 rpm for 10 min before being subjected to analysis through a UV spectro
Attached is the screen shot for the same with the bottom most spectra for blank sample and top most after 30 min degradation
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Probably the increase in absorption that you noticed is caused by the generation of by-products, due to the reaction of bisphenol A with i.e. hydroxyl radicals or other oxidising species. It means that the by-products show a larger absorbance than bisphenol A, because of a different extinction coefficient.
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How many chances of acceptance of a research paper if journals editor send it to review .
Especially for top journal indexed
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If it has passed editorial assessment than the research article has some scope which is required by the journal. After review you may get two options major or minor revision. But here the chances are 50:50 depending on the reviewers comments.
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I am looking for X-ray baggage screening dataset for Anomaly detection in X-ray security screening systems.
Thanks for your help.
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see
chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/viewer.html?pdfurl=https%3A%2F%2Farxiv.org%2Fpdf%2F2001.01293.pdf&clen=2380896&chunk=true
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Dear All,
I've been struggling with assembling a 30 kb (20 kb vector, AmpR + 10 kb PCR product) construct. Any helpful tips would be much much appreciated!
I have tried traditional RE (MluI/FseI) cloning, Gibson assembly (60 bp overlaps), and both strategies failed. I've tried electroporation with ElectroMAX DH10B, Stbl4, 10-beta from NEB for higher transformation efficiency. To give it one last try, I'm using NEBuilder HiFi DNA assembly, but it doesn't look promising. To improve screening (lots of AmpR false positives earlier), I also cloned a Neo/Kan resistance into my 10kb fragment in advance, and screened for colonies that are Kanamycin resistant.
As of now, I'm seriously considering subcontracting this project to a pair of more capable/experienced Molbio hands. Does anyone know a company that have expertises in making tailored large constructs, and have good experiences with it?
Best,
Hui-Min
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Sounds good. Although I would double check the vector backbone. The intermediate size seems ok, however, when you go bigger than that you will require backbones that have an active partition systems like in BACs.
Best of luck!
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We have screened the phytochemicals of Tinospora cardifolia and curcuma longa downloaded from Imppat database and Dr. Dukes database and targeted TGFbeta-R1 crystal structure using docking study and found one best compound from each quercetin from c.longa and tembetarine from T.cardifolia , Inorder ro test the compound to test invitro & invivo, we wish to purchase from chemical vendors but we found the compounds source from other plant species (Apis mellifera) but structure remains same for both cases. Is it a valid study if we conduct a study based on this
link : Quercetin ≥95% (HPLC), solid | 117-39-5 (sigmaaldrich.com)
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I think would be the same as suggested by other colleagues too. But, I think you must have to cite the source you taking the structure (s).
Thanks!
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As part of a research project for the early detection of the risk of post-/long-Covid, we would like to find suitable biomediators with high predictive power for screening diagnostics. For this purpose, a longer-term observational study in Covid patients is planned. A key objective of the planned study is to clarify an early indication for the rehabilitation treatment of Post-Covid syndromes.
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the test are largely based on four different analysis: reverse transcription polymerase chain reaction (RT-PCR), standart test for covid-19. ii, loop-mediated isothermal amplification (lamp). iii. lateral low- hand-held single use assays providing results for an individual patient in as short 15 minutes. iv. enzyme-linked ELISA immunosorbent assay.
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I need to buy Rhodamine B for lipase producing bacteria screening. They are available from Sigma-Aldrich in different forms such as HPLC, for fluorescence and standard analytical. Which one should I use?
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