Science topics: ObstetricsScreening
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Screening - Science topic

Screening, in medicine, is a strategy used in a population to detect a disease in individuals without signs or symptoms of that disease. Unlike what generally happens in medicine, screening tests are performed on persons without any clinical sign of disease.
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I am screening soybean genotypes for drought tolerance and vulnerability in a screen house. Some literature suggests 3 weeks and others suggest 4 weeks of drought imposition.
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Is there a way to perform the blue white screening using plates without the IPTG? Can I replace it with another substance?
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You can substitute IPTG with:
  • Lactose (e.g. 0.1–1% w/v in agar or liquid): natural inducer, cheaper (but gets metabolized so timing matters).
  • TMG (methyl-β‐thiogalactoside, ~1 mM): a non-hydrolyzable lactose analog that provides sustained induction.
  • Auto-induction media (e.g. ZYM-5052): contains glycerol, glucose (to repress early induction) and lactose (to auto-induce later), eliminating the need to add any inducer manually. In all cases, include X-gal on your plates as the colorimetric substrate for blue/white screening.
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join me
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AI has shown potential to outperform traditional screening tools in identifying depression, anxiety, and PTSD by analyzing complex patterns in patient data—such as speech, text, facial expressions, and biometric signals. Machine learning models can detect subtle signs often missed in standard assessments, enabling earlier and more accurate diagnoses. However, ethical use, transparency, and clinical validation remain essential for safe and effective implementation.
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I'm carrying out a qualitative systematic review on the barriers to screening uptake. Following my search and screening for primary studies to be included, I ended up with 5 primary studies. 3 of these studies stated that they used quantitative cross-sectional study designs, however after reading these three studies, I have realized that they provide qualitative evidence, that is, they discuss experiences and barriers to screening which is relevant to my review. Given that my review is qualitative, can I use the qualitative data from these quantitative studies, provided I state this in my selection criteria?
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Yes, you can use the qualitative data from those quantitative studies. In your inclusion criteria, state that you’ll include studies with qualitative insights, even if the overall design is quantitative. For example, mention: "Studies were included if they provided qualitative data on screening barriers, regardless of study design."
When extracting data, focus only on the qualitative parts (e.g., participant experiences). In your review, explain your approach clearly, such as: "Although three studies were quantitative, they provided qualitative data on barriers to screening, which was extracted for this review."
This ensures clarity and transparency in your methodology.
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I am planning to screen multiple GPCRs with different well-studied ligands. As a first check, I want to see if my GPCRs are expressing properly and are getting activated. Is there a quick and dirty way to test if it is doing what it is supposed to?
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Suthara,
For the proper expression in the plasma membrane an N-terminal tag would be the best with mAb you can buy.
For the activation, a working idea is Galpha16 or proper alternative and then looking for cytoplasmic Ca2+ signals, but you could also try a GTPgamma-35S assay, which directly shows you the activation of any GPCR.
Best, karoly
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How can we actively reduce children's screen time in our tech-driven society to promote healthier habits and better well-being?
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Establish clear guidelines, support offline activities, arrange screen-free zones, model healthy screen habits, and encourage interactive play and family time to balance children's digital screen time.
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I need to use the AutoDock program, but I tried both versions 1.5.6 and 1.5.7. I had no problem loading, my only problem is that when I open the program, the loading remains around 9% in the window that opens. Then the window closes with the command page that opens. It will be more understandable when I add screen shots. I am using Win11 operating system, I have an HP brand laptop, I really can't figure out where the problem is. In the screenshot I attached, it stays as it is. The program closes directly.
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I am having the same issue. It doesn't support windows 11?
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Nowadays, we are encountering with screen addiction in children especially during lockdown. I want to know any guidelines for screen control?
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Playing children with traditional paper-based games offers a valuable opportunity to promote educational development while simultaneously minimizing their use of digital screens.
One effective example is “Spot It! A Mathematical Approach to Offline Games,” which emphasizes the potential benefits of such activities in decreasing sedentary behavior and enhancing cognitive development in children.
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Hello, I'm facing a conundrum and was hoping to get some advice.
We have 12 cryo tubes (all replicates of the same clone) of HEK293T cells preserved in our -80 that have supposedly had a plasmid cassette inserted into the AAVS1 safe harbor locus site (I can provide more info on this if needed). I say supposedly because we're trying to verify this using various means, including PCR.
One of the replicates is showing promise in our PCR screenings. It should match one of the 12 replicates in the freezer. The problem is, I don't know which one. The genomic DNA we've been doing PCR screening with was labeled with well numbers, while the preserved cells only have the cell count and the clone number (they're all clone 1). Obviously this was an oversight we're now paying for.
I'm discussing with my PI how to go about screening them via western blot, which would involve thawing them all out, taking a portion for whole cell extract and another portion to re-preserve.
The preserved tubes range in cell count from ~3 x 10^6 cells/mL to just 1.55 x 10^4 cells/mL. For the tubes with lower counts, can they still be thawed in a T25 or do I need to use something smaller?
I just don't see any other way to go about this other than thawing them all out, but if I'm missing something, I'm open to suggestions! Thanks!
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If you know for certain that they're the same clone, then why do you need to screen them all? A clone by definition means they're genetically identical.
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whether journal editors are using any review AI tool for checking the paper quality for forwarding the decision to review committee or rejection of paper in first screening by the editorial team.
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AI detectors are poor western blot classifiers: a study of accuracy and predictive values
"The recent rise of generative artificial intelligence (AI) capable of creating scientific images presents a challenge in the fight against academic fraud. This study evaluates the efficacy of three free web-based AI detectors in identifying AI-generated images of western blots, which is a very common technique in biology. We tested these detectors on AI-generated western blot images (n = 48, created using ChatGPT 4) and on authentic western blots (n = 48, from articles published before the rise of generative AI). Each detector returned a very different sensitivity (Is It AI?: 0.9583; Hive Moderation: 0.1875; and Illuminarty: 0.7083) and specificity (Is It AI?: 0.5417; Hive Moderation: 0.8750; and Illuminarty: 0.4167), and the predicted positive predictive value (PPV) for each was low. This suggests significant challenges in confidently determining image authenticity based solely on the current free AI detectors. Reducing the size of western blots reduced the sensitivity, increased the specificity, and did not markedly affect the accuracy of the three detectors, and only slightly improved the PPV of one detector (Is It AI?). These findings highlight the risks of relying on generic, freely available detectors that lack sufficient reliability, and demonstrate the urgent need for more robust detectors that are specifically trained on scientific contents such as western blot images."
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Hi I am doing a systematic review and completed my title and abstract screen in March 2024. After that I took some break and want to continue that work. Please guide
1. Do I need to remove all the previous work and start with fresh one?
2. Can I add up new searches for last one year by applying Filter?
3. Can I search again and merge the two(old one and new)?
Thanks
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You can just update the search with the filter starting from the end of your initial search. (you can add a 6months overlap since some publications can be delayed in their apparition in some web search).
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I want to study the effects of a pharmacological treatment (antidepressants) related to quality of life in oncologic patients. Apart from a depression diagnosis that would be a prerequisite for administering the treatment, i need another screening tool that could confirm the patient's ability to be functioning enough to give me true and valid answers later in the main tests. For this reason I am looking for a validated tool in clinical setting that could detect any cognitive impairment due to a psychiatric condition or substance induced (es. high doses of morphine).
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Hello, I'm developing a tool that can help with that, it works with id and color pigmentation areas, one of the tool's modules can help with that. I'll be happy to help.
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I am currently conducting research for my master's dissertation, focusing on studying stress levels in poets. Therefore, I require a validated screening instrument to differentiate my target sample of poets from the general population. Please contact me, if there are any journals/research that you know of, that has this. Thankyou.
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Every person alive is a poet. The only criteria for being a poet is being alive.
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I can’t reset my
Password. Research Gate refuses them all. When it gives me a chance to reset them by pushing a button, the page won’t come up—only some letters to the left of the screen. Will you please fix this problem?
Ellie Ragland
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I have the same problem. Is it due to using browser on phone?
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I want to reset my password
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sorry. Dont know
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I prepped a cDNA library from ovarian cancer cell line RNA (all RIN scores were 9+ on TapeStation 4200) using a KAPA HyperPrep mRNA kit and UDI adapters for Illumina sequencing. When we ran the TapeStation (D1000 HS screen tapes) on the prepped libraries, we saw extra peaks around 230-250bp. On some samples it was a more pronounced peak, but others it was more of a shoulder. We are baffled on what this could be from. We did a QC sequencing run on an illumina nextseq P2 100 cycle and are wondering if our inner distance plot could look like this due to the smaller fragments we see on the tape station? I've had some people suggest the extra peak on the tapestation a bubble peak from over amplification of the library, but bubble peaks would appear larger, not smaller. Our anticipated library size was 200-300bp. For most samples the average size was 350bp, so including the adapter sequences this seems right. We just have no idea what the smaller peak would be that appear in nearly every sample. I've included images of a couple electropherograms and gel images from the tapestation and the inner distance plot. You can see these smaller extra peaks appear as bands on the gel images and how they slightly vary in size from sample to sample.
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Hi! It looks like an adapter dimer peak at 150 bp. Improve clean-up after adaptor ligation. Use x0.8 of beads, fresh-prepared 80% ethanol, pipette 5-10 times up-down during ethanol cleaning step.
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The intersection of biometric screening and artificial intelligence (AI) is a rapidly evolving field, with significant potential for innovation and improvement. Biometric screening involves the use of physical or behavioral characteristics, such as fingerprints, facial recognition, or voice recognition, to identify individuals. AI, including machine learning (ML) and deep learning (DL), can enhance biometric screening by:
1. *Improving accuracy*: AI can analyze large datasets to improve the accuracy of biometric matching, reducing false positives and false negatives.
2. *Enhancing security*: AI-powered biometric screening can detect and prevent spoofing attacks, such as using fake fingerprints or faces.
3. *Increasing efficiency*: AI can automate the biometric screening process, reducing the need for human intervention and increasing throughput.
4. *Enabling multimodal biometrics*: AI can combine multiple biometric modalities, such as face and voice recognition, to create more robust and secure identification systems.
Machine learning and deep learning are key technologies driving the advancement of biometric screening. ML can be used to develop algorithms that learn from data and improve over time, while DL can be used to analyze complex patterns in biometric data. Some examples of AI-powered biometric screening include:
- *Facial recognition*: DL-based facial recognition systems can accurately identify individuals in real-time.
- *Voice recognition*: ML-based voice recognition systems can authenticate individuals based on their unique voice patterns.
- *Fingerprint recognition*: AI-powered fingerprint recognition systems can improve the accuracy and speed of fingerprint matching.
Overall, the intersection of biometric screening and AI has the potential to revolutionize the way we authenticate and identify individuals, with significant implications for security, convenience, and privacy.
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The term ‘biometrics’ refers to the distinctive physical or behaviouralcharacteristics of an individual that can be exploited for the purpose of electronicidentification and authentication. Among the many types of biometric identifiers,some examples are fingerprints, face traits, speech patterns, and handwritingspeed. Each one of these identities can be used independently as a uniqueidentifier for a certain individual, and they can also be used in conjunction withone another to increase the reliability of identification. Today, biometrics arefinding an increasing number of applications across a wide range of industries.The utilisation of artificial intelligence (AI) in the field of biometrics israpidly developing, with the objective of enhancing the dependability, efficiency,and safety of biometric identification systems. (PDF) AI Based Advancements in Biometrics and its Applications. Available from: https://www.researchgate.net/publication/383551254_AI_Based_Advancements_in_Biometrics_and_its_Applications [accessed Jan 09 2025].
Regards,
Shafagat
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This question explores the intersection of computer vision and AI with physical game design. It seeks insights into using these technologies to create interactive, screen-free games that improve children's engagement, cognitive development, and social interaction while reducing reliance on digital screens.
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Computer vision and artificial intelligence technology can play a pivotal role in creating engaging physical games. Through image recognition by artificial intelligence that enables dynamic gameplay. By taking advantage of computer vision algorithms, I personally created games especially for my nephews using an Arduino controller and through the books I wrote that contain actual experiences in this field, I recommend reading that.
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I need clarification on the following: If we use ASRIVIEW Lab tool for screening of Articles for conduting Systemtic Literature Review, should we mention the tool name in the manuscript?
Thanks
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Yes all means used for achive ing the study should be mentioned
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Hi all, I plant to creat a yeast mutant with two gene knockout. I want to use homologous recombination method. I found lots people will knock out one gene first, then continue with the second one. Could I knock out these two genes with kanMX and natMX at same time, and screen the positive colonies in media containg G418 and nourseothricin?Thanks.
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I agree with Michael J. Benedik, the sequential KOs will be far easier to obtain. Also, won't you want the singles as comparisons in your assays?
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Hi everyone,
During my lab work, I sometimes have to look for the existence of some specific X-nucleus containing components and for this, I perform X{1H} NMR screening experiments (e.g 31P{1H} or 15N{1H}...). However I noticed that ''sometimes'' I don't get any X nucleus signal while when I use 2D [1H-X] HSQC or HMBC experiments on the same sample I do get signals. Has anyone of you encountered this issue before ? could it be hardware a related problem, knowing that all the performance control tests on the spectrometer, gave good results ?. Also, the SW window is always set wide enough to look for any potential signal.
Thank you.
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Dear Salem, for 15N direct observe experiments there are other factors to consider in addition to just sensitivity. The two major issues are heternuclear NOE and relaxation. Some nitrogen atoms can have very long relaxation times. In addition to acquiring a lot of scans you also have to use long relaxation delays. The other factor is heteronuclear NOE. 15N has a negative gyromagnetic ratio, therefor the maximum NOE can be -4. It is possible that the NOE is -1 and that leads to cancellation of the signal. For this reason it is often advised to use inverse gated decoupling (zgig) again with long d1's. Both 15N and 31P have a large shift ranges and the signal might not be where you expect it. If you have seen it in HSQC or HMBC you should know where to look.
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If we delay the arrival time of one of the two quantum entangled electrons from the monitoring screen, what will happen is that normally the two electrons must coincide (the arrival of the two electrons) at the same time to the monitoring screen. But what happened is that we delayed one of the two electrons, so what will happen is that it will happen, a contraction in the fabric of space-time so that the distance between the two electrons is equal. Therefore, the lagging electron passes through the contracting fabric of space-time between it and the monitoring screen, so that the distance between the two electrons is equal when they are observed. But because of that contraction, the delayed electron exceeds the speed of light and is quantum entangled with the other electron. Therefore, it will be in the past, and this indicates that if the electron is detected, it will affect the results of the experiment.
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In Entangled systems, time has stopped evolving. Meaning literally and clearly, time is ‘frozen’ for an entangled system, regardless of scope and scale.
That is the Ryu-Takayanagi Path selection as it is defined Figuratively “below” the AdS Horizon Surface: the Path (small) l connects points Alice and Bob, who are both at time zero. Meaning, Alice sends a message to Bob at time zero, the Information takes Ryu-Takayanagi Path l, however, the end of that Path l is connected to Bob, who is also and indefinitely at time zero.
Although Ryu-Takayanagi did not originally intend for that to be the case, that is the case. Time is Frozen in the AdS/CFT model for an Entangled system.
Meaning, there is no sequitur description of a “delay” in a system where time is not evolving.
Trying to explain this clearly observable thing with unobservable ‘contraction’ is primitive.
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Dear colleagues,
We are conducting a systematic review and meta-analysis of cross-sectional data, and I am looking for a suitable workflow management software. Those that I have worked with (RevMan) or explored (Covidence) seem to be focusing on intervention studies that do require a comparator group. We don't have this in prevalence data. Does anyone know of a software that can do the job? If open source, even better. Note that I am not looking for data analysis packages, data analysis will be done in R. What I am looking for is a program to upload the references from the search results for title screening, abstract screening, full text screening and eventually, data extraction. Thereafter, we will export to R.
Thank you in advance for your help, best, Fabian
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I think maybeVOSviewer?
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Zones which are greater use for quantitative screening. And don't know how to measure it's size.
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May I ask what kind of microorganisms are those on the photo ?
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We need to decrease the grain size of the mill scale in industrial scale to about 80mesh. for screening, which one is better vibration screen or rotary screens? also, about crushing machine which one is better, hammer crusher or ball mill?
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You are basically right and jaw crushers are used for coarser grinding and this is true, for example, when crushing stones. However, scale is a material that is ground very easily, produces a lot of dust and when ground in a ball mill will immediately produce a very large amount of too fine powder.
If the task is to obtain mainly particles of 100-200 microns, then a ball mill will cope poorly with this task. In addition, a ball mill is much more expensive than a jaw crusher of the same capacity.
On the other hand, there are small but productive jaw crushers that are designed for crushing to sizes less than 1 mm. When crushing scale in such a crusher, the output of particles of 100-200 microns will be large, and the amount of dust will be much less than after a ball mill. Using a combination of a jaw crusher plus a vibrating screen, you will get a good output of the desired fraction with high productivity and low costs.
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Dear research community What will happen if we rotate the slit in terms of the pattern on the screen? Will it remain the same or will it change?
Thanks
P.S The picture must be misleading What was meant to be asked was a kind of pattern that the screen will give if the plane is rotating and not just stays in a steady position
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Şekil doğru. Işık ekrana çarptığında güçlü olan foton parçacığı ekranda şekli oluşturur. Baskın olan rengin oluşturduğu şekil daha net ve önde görünür. Herhangi bir desenin iki merkez atoma sahip olması diğer dört atomun oluşmasına sebep olur. İlk cevapta anlatılan fırlatılan birinci foton veya elektron diğer şekillerin oluşmasına ortam hazırlar. İkinci foton ise gönderildiği zaman kuvvetli bir bağ oluşur. Bu nedenle kırmızı renk en önde ve daha net olur. Diğer renkler de aynı şekilde kırmızı renk sayesinde birbirine bağlanır. Bu durum gökyüzü olaylarında da olabilir. Örnek yıldızlar ve gezegenler vb. Bu sistem oluşurken artık merkez atom kararlı olur ve istediği gibi hareket edebilir. Sönümleme zamanında renkler zayıfladığı zaman oluşan küp veya dikdörtgen şekiller sırayla görevini yaparak kaybolur. Bu esnada kararlı olan atom en son ayrılır. En zayıf renk desenin üzerinde en son kalır ve sönerek kaybolur. Bu dikkatli incelendiğinde leke bıraktığı görülür.
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Dear Colleagues,
I am trying to identify a pathway in cellular system and I need to do a kinase inhibitory screen. The one from selleckchem is very expensive. Do you have experience with working with inhibitor library from other companies? Or have you tried other methods to identify (kinase) pathways? Thank you in advance, #Kinase #inhibitory_screen #High_content_screening #pathway #inhibitory_screen
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Hello Ehsan, you could have a try on the Kinase Inhibitor Library from TargetMol which containing 2720 kinase inhibitors/regulators. The price is very competitive. It can be used for research in chemical genomics, pharmacological study, and drug screening for related diseases.
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Dear colleagues
I have a GeneRead QIACube station which is specified for no longer supported Qiagen NGS GeneReader workflow (emulsion technology). It looks pretty much the same as casual QIACube, but has other worktable and screen. I just can't put a standard reagent tray into the device.
Is it possible to convert GeneRead QIACube (NGS sample preparation) into a QIACube for NA isolation?
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Hi …
Here are some considerations:
Potential Options
_Contact Qiagen:The best approach would be to contact Qiagen directly. They can provide specific guidance on whether any modifications can be made or if there are any recommended workflows for your device.
_Modification: If you're technically inclined, you might explore whether you can modify the reagent tray or worktable to fit standard trays. However, this could void warranties or cause operational issues.
_Alternative Solutions: If conversion is not feasible, consider using the GeneRead QIACube for its intended purpose or investing in a standard QIACube designed specifically for nucleic acid isolation.
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I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes (positively selected) but not the negatively selected ones. On the terminal, I get this error:
INFO  @ Mon, 29 Jul 2024 11:17:57:   Error in plot.window(...) : Logarithmic axis must have positive limits
INFO  @ Mon, 29 Jul 2024 11:17:57:   Calls: plotrankedvalues -> plot -> plot.default -> localWindow -> plot.window
INFO  @ Mon, 29 Jul 2024 11:17:57:   In addition: Warning message:
INFO  @ Mon, 29 Jul 2024 11:17:57:   In xy.coords(x, y, xlabel, ylabel, log) :
INFO  @ Mon, 29 Jul 2024 11:17:57:     1 y value <= 0 omitted from logarithmic plot
INFO  @ Mon, 29 Jul 2024 11:17:57:   Execution halted
I would be very thankful if someone with a similar experience could help solve this issue.
Thank you,
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I found the solution to the issue. The problem was caused by a small formatting error in the gRNA list file. Specifically, there was a space between the gene name "septin" and the number "5" in the entry for "septin 5". It should be "septin5" without a space. This minor formatting error prevented the data from being processed correctly, leading to the plotting error. It took a long time to identify this issue, but it's resolved now. I'm sharing this in case someone else runs into the same error.
Best,
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Hi, I'm going to use ReFinder to screen housekeeping genes, but I can't access to the Blooge Refinder or the (https://localhost/RefFinder-master/index.php) via XAMPP.
Does anyone has ReFinder access resourses that can be shared, please?
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I am doing a gene editing experiment and I want to edit two sites at the same time, so there are two resistance genes, can I add two antibiotics to the cell after transfection 72 hours or do I add one antibiotic to the cell first and then add the other antibiotic to the screen? Is it possible that screening after two weeks of transfection has no effect?
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You can use two antibiotics at the same time although depending upon the antibiotics there is some synergistic stress incurred by the cells due to having two drugs. So just be aware.
However do you need to both edits at the same time? It might be easier to do them sequentially if possible? Because depending upon efficiency of your experiments the frequency of getting both is certainly going to be lower than getting one.
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Dear all,
We are producing activated carbon from hazelnut shells but have an issue about the dust of the a.c. granules also known as the dust film on granules. Linear vibrating seives doesn't do the job and granules are still dusty after seiving even multiple times. Dusty granules darken the water which is undesirable for water treatment applications. Can you please suggest any machine or a solution?
Thanks.
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Emil Aliyev its a nice thoughtful question where yes you are absolutely correct about the activated carbon granules can accumulate dust and impurities over time, reducing their effectiveness. To dedust activated carbon granules, follow these steps:
first can be Sieving: Pass the granules through a mesh sieve or a fine-mesh strainer to remove any lumps and large particles.
Next it can be Air blowing: Gently blow compressed air through the granules to loosen and remove surface dust.
Similarly after airblowing it can be Vibration: Place the granules in a container and vibrate it gently using a vibrating screen or a plate vibrator to dislodge dust particles.
followed by Centrifugation: Spin the granules in a centrifuge to separate dust and impurities from the granules.
and this can also be removed by Rinsing with water: If the activated carbon is water-resistant, rinse it with distilled water to remove any remaining impurities.
and finally one has to look into the Drying: Dry the granules in a low-temperature oven (150°F - 200°F) or under sunlight to remove any moisture.
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Covidence seems to have have very strong relevance in the medical and health sciences when doing a literature review. I am currently working on Students well-being in higher education using covidence for the texts screening. I have not been able to figure out the relevance of the quality assessment template in my data extraction stage.
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The quality assessment template in Covidence, a tool used for systematic reviews and research synthesis, may have limitations in humanities and education domains research. While Covidence is widely used in healthcare and medicine, its quality assessment template might not fully capture the complexities and nuances of research in humanities and education.
Humanities and education research often involves qualitative studies, mixed methods, and diverse methodologies, which may not fit neatly into the template's categories. Additionally, the template's focus on bias and risk of bias might not be as relevant in humanities and education research, where context, interpretation, and perspective are crucial.
Researchers in these fields might need to adapt or modify the template to suit their specific needs or use alternative quality assessment tools that better align with their research paradigms.
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I have tried to screen various phytochemicals of different plants leaves sample with antimicrobial property. I have tried aqueous extract of plants. But i have doubt whether sample is actually showing all of phytochemicals present in plant sample. How to screen whole of alkaloids, flavonoids, terpenoids or phenolics present in the plant sample ?
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first and foremost, the phytochemical screening of a plant varies depending on the type of extraction carried out. if you are using the same plant, for example, you expect to have the same constituent depending on the solvent being used. You said you used an aqueous extract of plants, right? Which solvent did you use to prepare the aqueous extract? Please note that Aqueous extract usually contains higher polar solvents because, in the preparation of the solution for extraction, you are adding water to a certain ratio of solvent to water. Mostly, the ideal ratio is 70:30, i.e., solvent 70 and water 30 %. When it comes to the issue of alkaloids, flavonoids, terpenoids, and phenolics, using standard methods such as the Evans and Tresa method, you can check if these phytochemicals are present or not; this is qualitative phytochemical screening. Sometimes we get false positives when we screen for alkaloids in a plant material, so it is best to do a specific extraction for alkaloids and then carry out qualitative phytochemical screening on it to confirm if they are present. I can refer you to one or two of my articles in which I carried out qualitative and quantitative phytochemical screening of a plant.
-Dauda G, Ali BH, Muhammed SY, Muhammad MG, Ismail AM, Muhammad MA, Hassan HS. 2023. Qualitative and quantitative phytochemical profiling of ethnomedicinal folklore plant-Globimetula oreophila. J. Curr. Biomed. Res. 3, 1407-1426.
-Dauda G, Haruna AK, Musa AM, Hassan B, Mohammed IM, Magaji MG. 2016. In-vivo antimalarial activity of ethanol leaf extract of Globimetula oreophila (Hook. F) Danser Azadiracchta Indica. Biol. Environ. Sci. J. Trop. 13, 55–59.
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The protein was expressed using the insect cell system, and the recombinant plasmid was successfully constructed as verified by sequencing, and the recombinant plasmid was transformed into DH10 receptor cells for screening of blue and white spots, and the selection of white spots was verified by PCR reaction, and agarose electrophoresis showed that all of them were negative.
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The white spots selected were all untransposed.
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I am trying to find the Hansen solubility parameters for screening miscible substances. Could anyone suggest an open-source online tool to calculate the Hansen Solubility parameter or any other equivalent solubility parameters?
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kindly have you cross the solubility parameter, did you get it ? if yes help me so, thanks
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Dear scientists,
Faced now with the rediscovery of already known compounds, which natural source do you think would be best for screening and isolating new bioactive molecules?
Nice day!
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Some very interesting antibiotics have been discovered being produced by unculturable soil microorganisms and previously unstudied microbiomes. See, for example, work from the lab of Kim Lewis at Northeastern University.
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We have two quant studio 3 real time pcr machines, no service contracts. One of the machines can run an experiment and the touch screen does work; however, none of the touch screen features work. This means we cannot open the drawer for the 96 well plate.
Has anyone had this issue or have troubleshooting experience with this?
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Sometimes, there is a communication issue between the machine and the laptop and you may need to reset the PCR machine.
However, if it is your touch screen on the pcr machine, we have found you can connect a usb mouse to the front port and use the screen functions that way.
Hope this helps!
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I have conducted phytochemical screening followed by FTIR for an aqueous plant extract and I want to know how to interpret the FTIR results to determine which phytochemicals are present based on the functional groups that have been determined/predicted by FTIR. Is there any other way to interpret the results?
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As you will be aware, plants are a very rich source of phytochemicals and this will be reflected in your aqueous extract. The IR spectra of your extract will be complex, containing the overlapping spectra of every compound. You will not be able to identify any individual compound with any degree of certainty.
If you want to identify the components of your extract then you need to resort to a Metabolomics type experiment. You need to add in several chromatographic steps and then resort to GC-MS (headspace for volatiles), after appropriate derivatisation, and to LC-MS. The spectra can be searched against various libraries. Even then you will be left with many unknowns.
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Search for article.
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found that males displayed larger head and neck flexion angles than females, which were associated with the amount of computer use.
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Hello I'm an engineer new to structural biology and helping to develop a cloud docking tool for screening compounds, similar to Swissdock but with mass throughput and GPU optimizations.
Specifically we're helping researchers repurpose existing drugs against protein structures simulated from the novel coronavirus genome.
I'm planning to use GPU optimized AutoDock-GPU , which takes in <protein>.maps.fld
I know you can use autogrid to select the bounding box and generate the .maps.fld, but I've been unable to figure out the workflow. Also for preliminary screening I want to search the whole protein without specifying a bounding box. Is there a script for converting protein.pdb to .maps.fld?
Thanks!
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sorry to bother you but i have tried a lot and i didn't find the answer yet
I'm trying to use autodock GPU on colab but just for one ligand and and receptor
i find that my protein should be in .maps.fld format
but mine is in PDB or PDBqt could you help me if you find a way ?
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Professors, much like artists or musicians, wield chalk as a tool of expression and creativity in the classroom. In the act of lecturing, the chalk becomes their paintbrush or instrument, allowing them to dynamically articulate ideas and illustrate concepts on the canvas of the blackboard. The visual composition created through the arrangement of text, diagrams, and illustrations resembles the work of artists or the musical score of a symphony.
Lecturing with chalk is akin to a dynamic performance, where professors move fluidly, engaging students in a live and interactive manner. This performance aspect adds a sense of dynamism to the learning environment, much like a musician on stage captivating their audience. The tactile engagement with chalk further deepens the connection between the educator and the material, reminiscent of the intimate relationship between a musician and their instrument.
Professors, in their creative interpretation of subject matter on the chalkboard, make choices akin to artists interpreting a theme or musicians interpreting a score. The use of colors, emphasis on certain points, and the improvisational nature of the lecture contribute to a unique and creative interpretation of the content. The interaction with students during a lecture mirrors the engagement between musicians and their audience, creating a collaborative atmosphere that enhances the educational experience.
Beyond the practicality, the act of using chalk on a blackboard carries symbolic significance, connecting professors to a tradition deeply rooted in the history of education. This symbolic gesture aligns them with a legacy of knowledge dissemination, much like the choice of specific tools by artists for their symbolic value. In essence, professors wielding chalk as an artistic or musical tool enrich the teaching and learning experience, infusing it with creativity, expression, and a sense of tradition.
Chalk and blackboards have been a traditional and iconic combination in education for centuries. Some unique features that make them stand out as tools of trade, particularly for teachers and educators are:
  1. Versatility: Chalk is a versatile medium that can easily create both bold and fine lines. It allows for quick writing and drawing, making it suitable for a variety of purposes, from writing notes to illustrating diagrams.
  2. Interactivity: Blackboards provide a dynamic and interactive platform. Teachers can write, erase, and modify content in real-time during a lesson, engaging students actively in the learning process. This interactive aspect is crucial for classroom communication and participation.
  3. Cost-effectiveness: Chalk and blackboards are relatively inexpensive compared to modern digital alternatives. They are affordable for educational institutions, making them accessible in a wide range of settings, including schools with limited resources.
  4. Tactile Experience: The tactile experience of writing with chalk on a textured blackboard provides a sensory element to the learning process. Some educators believe that the physical act of writing helps reinforce memory and understanding.
  5. Visibility: The contrast between the white chalk and the dark blackboard background enhances visibility, making it easier for students to read and follow along. This clarity is important for effective communication in a classroom setting.
  6. Durability: Chalk and blackboards are durable and have a long lifespan when compared to some digital devices. They don't require electricity, and with proper care, blackboards can last for many years.
  7. No Technical Barriers: Unlike digital tools that may require technical expertise or face technical issues, using chalk and blackboards is straightforward. They don't rely on power sources, software updates, or other technical considerations.
  8. Nostalgia and Tradition: Chalkboards evoke a sense of nostalgia and tradition. Many people associate them with their school days and consider them an enduring symbol of education.
But subjectively speaking, professors, like artists and musicians, often develop a unique relationship with the tools of their trade, and for educators, chalk and blackboards hold a special place in their pedagogical approach. Here are some subjective perspectives on how professors might relate to these tools:
  1. Expressive Medium: Just like artists express themselves through various mediums, professors see chalk as a tool that allows them to convey their thoughts and ideas dynamically. The act of writing on a blackboard becomes a form of expression and communication, enabling them to illustrate concepts vividly.
  2. Creative Freedom: Professors may appreciate the creative freedom that comes with using chalk and blackboards. The ability to draw diagrams, sketch illustrations, and emphasize key points in real-time provides a level of spontaneity and creativity that can be challenging to replicate in a more structured digital environment.
  3. Personal Connection: Some educators feel a personal connection to the tactile experience of writing with chalk. The physicality of the process, the sound of chalk against the board, and the immediate feedback from students contribute to a unique teaching experience that fosters a personal connection between the professor and the material.
  4. Engagement and Presence: Just as musicians connect with their instruments, professors may feel a sense of engagement and presence when using chalk and blackboards. The ability to stand in front of the class, interact with the board, and gauge student reactions in real-time can enhance the overall teaching experience.
  5. Time-Honored Tradition: Professors may appreciate the time-honored tradition associated with chalk and blackboards. It can evoke a sense of continuity with the past, connecting them to generations of educators who have used the same tools to impart knowledge.
  6. Intimate Classroom Atmosphere: Some professors prefer the intimate atmosphere created by a traditional chalkboard setup. The physical proximity to the board and the ability to move around the classroom contribute to a more intimate and interactive teaching environment.
  7. Adaptability: Chalkboards allow for quick adaptability and improvisation during a lecture. Professors can easily erase and modify content based on student questions or the flow of the discussion, similar to how a musician might improvise during a performance.
  8. Symbolic Meaning: Chalk and blackboards can carry symbolic meaning for educators. The act of writing and erasing can represent the iterative process of learning, and the visible presence of the board serves as a constant reminder of the ongoing exchange of ideas in the classroom.
While technology continues to play a significant role in education, the subjective experiences and preferences of professors contribute to the enduring appeal of chalk and blackboards in many academic settings.
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Tieu-Tieu Le Phung , it is about art.
"On some level, theorists at a blackboard resemble magicians, philosophers, and athletes. They also have something in common with artists, producing intellectual artworks that seem designed for Instagram feeds. As you walk through the London Institute’s exhibition of blackboards, you’re struck by their austere beauty..."
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While performing STD screening I noticed that I have signals in my difference spectras, that are constant accross all samples, also if there is no ligand and only protein.
The protein was purified via size exclusion and is in deuterated PBS.
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Are the signals from the protein itself or from something else?
The subtraction doesn't always remove the protein signals completely, and in that case you can use one of the pulse sequences that uses a spin lock to destroy the protein signals. On Bruker systems these are the std pulse sequences ending in .3
If the signals are from something else, you could try to run a cpmg experiment or similar to see what they might be.
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Hello
I m in need of protocol how to generate database in phase flr screening? The help from anyone is appreciated.
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Please use Phase database creation module of the Maestro to geneerate phase databases from the downloaded database.
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help me to cite pls. for our research purpose
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1. define excessive. 2. screen time = caregiver doesn't want to deal with kid. If parent is poisonous, all screentime is good and vice versa.
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I have the data on the DBH and the age of the trees. The farmers provided the ages, and some of them are not related to the reality of the DBH. Is there any scientific method that I can use to remove some ages that I can consider false data or outliers? Methodology: I can use DBH and ages to screen some data.
Thank you
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To screen data using Diameter at Breast Height (DBH) and age, begin by collecting precise measurements of tree DBH and age requires at least 3 years of data. Establish criteria based on your analysis goals, such as specific age ranges or DBH thresholds. Apply these criteria to filter the dataset, selecting trees that meet the specified conditions. Utilize visualizations like scatter plots to explore the relationship between DBH and age. Conduct statistical analyses, such as correlation or regression, to uncover patterns in the data. Perform quality control checks to ensure accuracy, identifying and addressing outliers. Finally, interpret the results in the context of your research objectives, considering how the relationship between DBH and age aligns with expectations or hypotheses. Adjust these steps according to the unique goals and characteristics of your study.
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I am conducting a research on mental health issues and help seeking behaviour of postpartum mothers with babies in Premature baby units and neonatal issues. I would like to know of a tool helpful to screen these mothers.
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You could use the Edinburgh Postnatal Depression Scale (EPDS)- https://med.stanford.edu/content/dam/sm/ppc/documents/DBP/EDPS_text_added.pdf
Or if you'd like to just check their subjective wellbeing you could use the Subjective Wellbeing Scale developed by the World Health Organization. I have published an article where I have used this scale.
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please help......I need this infromation ASAP
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Here are some notable advancements along with their pros and cons:
1. Dual-Energy X-ray Absorptiometry (DXA):
- Pros: DXA is considered the gold standard for measuring bone mineral density (BMD) and diagnosing osteoporosis. It is widely available, relatively low-cost, and exposes patients to low radiation doses.
- Cons: DXA primarily measures BMD and may not fully capture bone quality and fracture risk. It also has limitations in distinguishing between trabecular and cortical bone.
2. Quantitative Computed Tomography (QCT):
- Pros: QCT provides volumetric BMD measurements and can differentiate between trabecular and cortical bone. It is useful for assessing bone strength and fracture risk prediction.
- Cons: QCT involves higher radiation doses compared to DXA. It is less widely available and more expensive. Additionally, QCT scans may have lower spatial resolution compared to DXA.
3. High-Resolution Peripheral Quantitative Computed Tomography (HR-pQCT):
- Pros: HR-pQCT provides detailed three-dimensional imaging of bone microarchitecture. It allows for assessment of trabecular and cortical bone compartments, and evaluation of bone strength and fracture risk.
- Cons: HR-pQCT is limited to peripheral skeletal sites (e.g., wrist or ankle) and is not suitable for central skeletal assessment. It is relatively expensive and less accessible than DXA.
4. Trabecular Bone Score (TBS):
- Pros: TBS is a texture analysis technique applied to DXA scans that provides information about bone microarchitecture. It complements BMD measurements in assessing fracture risk.
- Cons: TBS is an indirect measure of bone microarchitecture and relies on DXA scans. It may not be as informative in certain clinical conditions or when DXA scans are of low quality.
5. Biochemical Markers of Bone Turnover:
- Pros: Measurement of bone turnover markers (e.g., serum levels of osteocalcin or CTX) can provide insights into bone metabolism and response to treatment. They are useful for monitoring bone health and treatment efficacy.
- Cons: Bone turnover markers are influenced by various factors and may not provide a direct assessment of bone strength or fracture risk. Their clinical utility is more limited compared to imaging-based techniques.
Hope it helps: credit AI
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Dear colleagues,
A research team is conducting a study to evaluate emotions that have been automatically generated by using GANS, through a small survey. The survey presents 20 works of art in four different versions, each aimed at evoking one of the following emotions: amusement, delight, dread, and melancholy.
Your opinion is highly valued. Kindly access the form provided via the link and indicate the emotion you perceive each one of the 20 works of art to evoke. We suggest increasing the screen brightness to have a better view of the images.
Thank you for participating in this research. Your responses will be greatly appreciated. Feel free to share with your contacts.
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I'm not sure, as we will need to analyse the data and write the paper over the next year.
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I am trying to find any research related to mass health screening of general populations anywhere around the world.
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Probably the largest screening programmes worldwide are the cervical cancer screening programmes. There is a wealth of information about the screening programmes and I would start with national programmes such as the UK Cervical Cancer Screening Programme:
as this has a variety of information on the programme and how it works as well as links to recommendations and the research which backs it up. There are also links to the National Screening Committee which makes the recommendations and through that archives of all the research and reports that show how the programme works. Ireland has a similar, if slightly younger, programme which is probably worth a look too, on the basis of your location, and has worked closely with the UK in its development.
There is a wealth of data on cervical screening and it is one of the oldest programmes going and has a screening population of many millions in a country like the UK with a universal healthcare system.
Hope this helps.
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Protocol for Screening Low-Density Polyethylene (LDPE)–Degrading Soil Fungi Isolated from Urban Waste Dumping Sites | SpringerLink
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When aligning the objective aperture on a F30 in diffraction mode, does the electron beam need to be in the center of the screen? For example look at the picture I have shared. The beam is off centered wen in diffraction mode. Do I need to put the beam in the middle or is it okay if it's not?
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Var St. Jeor Thank you. Just to clarify, the microscope that I am using is the FEI Tecnai G2 F30 Field Emission Gun Transmission Electron Microscope
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Is there different solutions and new technologies or research on how we can help on this matter.
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In developed countries, a variety of safety measures ensures a low risk of transfusion-transmitted infections. These safety measures include donor selection (limiting imported and window infections); skin disinfection and diversion bags (limiting bacterial contamination during blood donation); the screening of donations (enabling timely detection of HBV, HCV, HIV, and Treponema pallidum); specific processing (such as leukodepletion, pathogen reduction, and inactivation, which remove or kill certain pathogens); quarantine plasma (preventing window infections); bacterial culturing (detecting contaminated platelets); and post-donation and post-transfusion notification (respectively, enabling donor- and recipient-triggered look-back procedures, which identify infected recipients).
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I have a highly conserved pocket in my enzyme that currently has no known function. I'm interested to see whether it binds a particular molecule. I'm wondering if anyone knows of an AI or software that can screen the pocket and identify molecules that are likely to bind there.
Thanks!
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Please check this "Protein-protein binding site identification by enumerating the configurations"
which may help you.
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Hello researchers,
I am a student at the SOMT, university of Physical Therapy(The Netherlands) and I am studying for a master degree in Geriatric Physical therapy.
Currently I am working on a school assignment where I want to look further in differentiating sarcopenia (screening) in a nursing home. In a lot of literature I found that the Ishii screening tool is highly
recommended to use. Unfortunately I can't find this tool? This is maybe a bold question but can you maybe help me by telling me where I can find this exact tool?
It would really help me with my assignment :)
Thank you- anyway and also thank you for all your research and very interesting studies.
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Hi,
You can find it on the following link :)
Hope that helps!
Best of luck from India!
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Is it more acceptable or tolerable of false positive or false negative in suicide screening for a suicide screening tool?
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I think goo more tolerate false positive than tolerating false negatives. Tolerating false negative could be dangerous.
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UNKNOWN DRUGS AND METABOLITES SCREENING USING LCMS
FROM SUSPECTED SAMPLES
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First establish a method (HPLC, then LC-MS) which follows good fundamentals for a series of expected known drugs and metabolites. *You can not develop a method for "unknowns".
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I want to develop an understanding for conducting Meta-Analysis studies in Psychology. how many studies to select, how to screen them, any tools which are used for the purpose. kindly explain with references how to conduct a meta-analysis
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Kindly refer my articles for conducting meta analysis.
I have collected articles in ten electronic databases - Springer, Elsevier, taylor and francis..etc., and used PRISMA guidelines.
refer this article
Shamseer, L., Moher, D., Clarke, M., Ghersi, D., Liberati, A., Petticrew, M., ... & Stewart, L. A. (2015). Preferred reporting items for systematic review and meta-analysis protocols (PRISMA-P) 2015: elaboration and explanation. Bmj, 349.
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I need a set of images of emotional faces (angry or sad; neutral; happy) of infants. I would like to frontally present these faces on a screen in a computer experiment. If infant images were matched in size, luminance, position, etc, with other images of adults, it would be great! Yhank you
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Hi, I do realise this is an incredibly late reply but if you are still looking for resources, I have used the CAFE database.
LoBue, V. & Thrasher, C. (2015). The Child Affective Facial Expression (CAFE) Set: Validity and Reliability from Untrained Adults. Frontiers in Emotion Science, 5.
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My lab has the NEB Gibson Assembly kit (unfortunately not the Hifi kit) and I have been struggling for months to assemble various different constructs. I'm wondering if anyone has used this kit specifically and if there are any specific things/tricks you do or use?
I have tried to insert 3 fragments (ranging between 500 bp - 2 kb) into a vector backbone, as well as overlap PCRs to insert 1 fragment into the vector. All fragments have between 30-40 bp homology regions. I PCR amplify all of my pieces (including backbone) and gel extract them because we don't have DpnI. I can get the overlap PCR working well, so I thought the primers/homology regions were designed ok. Yet, I can't get a simple 1 insert into 1 vector reaction working - I consistently get zero colonies (occasionally I've gotten an absurdly low number like 2 or 4, but screening them they're false positives).
I've used NEB's positive control to make sure the Gibson mix is working and got many colonies, but unfortunately they don't provide much information on the contents other than it's 2 DNA fragments so I'm not sure how they designed the two fragments to be ligated.
I have typically been adding 50 ng vector and tried both equimolar amounts of insert and 2-3 fold molar excess of insert. Is there a concern that there is too much salt contamination carried over from the gel extraction? I was curious about the positive control NEB provides and nanodrop read quite comparable A260:A230 ratio between that and my fragment mix. I've also tried adding 5% DMSO into the reaction in case secondary structures were preventing efficient assembly, as well as transforming 50 uL of NEB10b with 2uL vs. 10uL of the Gibson reaction. And nothing seems to stick.
Sidenote I tried IVA once and didn't work for me, but if you have any specific recommendations with that I'd also be willing to try troubleshoot that method if I continue to have issues with Gibson.
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This is a terrible kit, the hi-fi builder is much better at the job. Gibson kit requires more than 6 hrs or even overnight to get the proper assembly done while cloning multiple fragments. Since you are getting assembly products using the overlap extension PCR you can do some things:
1) amplify the fused overlap PCR product using 5' phosphorylated primers/or phosphorylate them after PCR using T4 PNK and then use it for blunt end cloning.
2) try to amplify the vector and. fused product with homology to each other and gel purify/Dpn1 treat them and then directly transform in dh5a, the e coli joins the homology ends in a recA independent homologous recombination reaction within. (One of the easiest and fastest cloning methods I have used to date.)
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According to an article entitled "Blood Transfusion Safety, current status and challenges in Nigeria" (2017), achieving blood transfusion safety in Nigeria (and likely the rest of Sub-Saharan Africa) remains a difficult task due to a number of factors, including a lack of blood, inadequate implementation of blood transfusion guidelines, insufficient infrastructure, and a high prevalence of transfusion-transmissible infections (TTIs), particularly hepatitis and human immunization. Even though there has been a slight improvement, particularly in the area of screening donor blood for common TTIs, significant efforts are still required in the form of effective public education campaigns (on blood donation) and ongoing system improvement to move the nation's current transfusion practices in the direction of safety and self-sustenance.
Source: Aneke JC, Okocha CE. Blood transfusion safety; current status and challenges in Nigeria. Asian J Transfus Sci. 2017 Jan-Jun;11(1):1-5. doi: 10.4103/0973-6247.200781. PMID: 28316432; PMCID: PMC5345273
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The following procedures must be followed when handling serum in order to guarantee the highest level of safety for blood transfusions:
A. Donor and recipient ABO and D groupings
- Both the donor and the recipient should have this determined precisely.
B. Extended donor and recipient red cell phenotyping
- In addition to ABO and D antigens, appropriate anti-sera are used to identify other antigens that can lead to immunological sensitizations and reactions, such as: C, E, c, e, K, k, etc.
C. Receipt serum antibody detection and screening
- Standard cell panels should include group O red cells that have been carefully phenotyped and contain all of the antigens that are known to cause transfusion reactions in the area, including D, C, E, C, E, K, K, etc. Additionally, the panels need to contain both homozygous and heterozygous cells for the phenotyped antigens. To demonstrate the dose impact during the antibody identification method, this is required.
Reference:
Ahmed, S. G. (2022). Transfusion services in tropical Africa: Challenges and prospects from the Nigerian perspective. Niger J Haematol, 3, 1-17.
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Given the disproportionately high risk provided by blood-borne pathogens, regular monitoring of blood transfusions is required to avoid disease transmission. ELISA and Rapid diagnostic immunochromatographic technique are the two most extensively used methods for detecting HBV, HCV, and HIV infection in blood donors. Although ELISA are considered more accurate worldwide. With this, what are the drawbacks of using the rapid immunochromatographic kits for screening blood donors compared to ELISA?
Source: Al-Matary, A. M., & Al Gashaa, F. A. S. (2022, November). Comparison of different rapid screening tests and Elisa for HBV, HCV, and HIV among healthy blood donors and recipients at Jibla University Hospital yemen. Journal of medicine and life. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9762378/#:~:text=This%20study%20showed%20a%20significant,infectious%20markers%20for%20blood%20donors.
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The use of rapid diagnostic immunochromatographic technique (ICT) methods are prevalent in developing countries, while ELISA and molecular testing are recognized as more widely accepted for their enhanced accuracy. Rapid diagnostic tests for viral infections have been available since the 1990s, primarily designed for emergency diagnostics, home-based testing, and field surveys. Furthermore, in several deprived regions, rapid diagnostic tests are used as a means to identify infections caused by the hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV). This method serves as a solution to address the scarcity of financial resources and medical equipment. Nevertheless, a significant issue regarding the utilization of rapid screening tests revolves around the necessity for these tests to demonstrate an acceptable level of sensitivity and a sufficient level of specificity to reduce the occurrence of false results.
Reference: Al-Matary, A. M., & Al Gashaa, F. A. S. (2022). Comparison of different rapid screening tests and Elisa for HBV, HCV, and HIV among healthy blood donors and recipients at Jibla University Hospital Yemen. Journal of Medicine and Life. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9762378/
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In responding to a request for a text I've always clicked on "View Request" and then get a screen where I can choose to respond or not. I don't get that anymore -- it's just an abstract of the paper they've requested. I can find no way to send the text. Please advise. Thanks.
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I remember that several users had problems with this at the time you asked. Has the problem been fixed meanwhile?
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I have try to establish a CRISPR screen in primary T cells,sgRNA transduce with
lentivirus and Cas9 protein delivered by electroporation (SLICE). But in the pilot experiment, we found that the efficiency of knocking out CD45 through this system is very low. The same experiment on Jurkat also failed.
Then I tried to change the experimental conditions, for example, increasing the amount of Cas9 protein or using Cas9 mRNA, or change CD45 sgRNAs.
None of these attempts have improved CD45 knockout efficiency.
I would appreciate it if you could give me some good advice!
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Thanks for your advice!
Because our library is a single sgRNA in lentiGuide-puro plasmid, so we just try the same backbone with a single CD45 sgRNA, but if there is a library containing 2 sgRNA per plasmid, we could try it.
For your second advice, in fact, before proceeding with electroporation Cas9 protein, we performed a drug screening with puromycin.
Thanks again for your valuable comments and look forward to your reply!
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Oral cancer has a high morbidity and mortality rate and clinical examination has resulted in late diagnosis. What reason has led to a delay in the implementation of a complementary screening test to the conventional clinical test?
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Dear Malcolm Nobre I really appreciate every response lead and I'm sorry not to lead a debate so as not to influence new responses. Biomarkers are very interesting, really, but would they allow us to say where the cancer actually is in a molecular stage, let's say (on the tongue? on the soft palate? on the floor?) and how to identify the real location at a stage that biomarkers theoretically allow to identify , at the molecular level or would we have to know that we have a probability of oral cancer and then wait for clinical evidence later to locate the tumor? . Westra explains this issue well. Do other methods in other areas really have great sensitivity or specificity or would it be the approach that facilitates, for example, being able to remove the entire tissue where, in the mouth, we do not have such an approach to remove the entire oral cavity.I really appreciate every direct response about oral cancer screening. who knows, one more question: could it be that many studies no longer confuse screening with diagnosis and brilliant works are lost by a mere confusion of basic concepts? very grateful
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covidence a screening software, and for full text screening if i want to keep article for my supporting document but not include in study how should i flag it?
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for such problems, it's important to contact the covidence support team for assistance.
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Some papers refer to calculate FRAP value as
FRAP value = = [(A1 − A0)/(Ac − A0)] × 2, where Ac is the absorbance of the positive control, A1 is the absorbance of the sample, and A0 is the absorbance of the blank.
Some other papers refer to calculate FRAP value as
FRAP value will be Trolox equivalent using Y=mx+c equation.
Please let me know the original process for the FRAP value calculation.
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I have conducted the second method in my research. You may refer to my paper
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Hello, everyone!
I am looking for a dataset where people recorded the simplest possible reaction time task. Something like pressing a button as soon as the screen turns red. Or maybe any variations that are not too far from such an experiment.
I know research on reaction time has been going on for the past ~40 years. But somehow I can not find a dataset for it. Wonder if any of you have seen it?
Thank you in advance!
Vitalii.
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I'd be emailing the corresponding author of whichever paper describes the sort of dataset that's most relevant to you - for example, someone who has published on "intention to move" vs. muscular response time.
Many journals nowadays require that data be made available upon reasonable request, and I'm sure there are many researchers out there who'd be happy to help you?
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I need to screen the toxicity of several compounds that will be applied locally to an open wound of bone and muscle injury, which human cell lines (good for several passages) would be most appropriate for this experiment beside hMSC?
Thanks
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for high throughput screening, how about "Establishing cell line" using bone marrow-derived mesenchymal stem cells since they possess both myogenic and osteoblast potential?
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I have already used the Capture SELEX method for the screen of small molecules.
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Aptamers have a wide range of targets, and the size and solubility of the targets are different. Therefore, the application of SELEX technology to screen nucleic acid aptamers can adopt different specific operation methods. Centrifugal precipitation is often used for aptamer screening of cells, bacteria and viruses, and solid-phase adsorption and elution technology can be used for aptamer screening of soluble small molecules. Specific methods for aptamer screening include the following aspects.
Screening methods based on different fixation media. Nitrocellulose membrane filtration is a commonly used method for protein aptamer screening.
Joshi et al. designed a lateral flow chromatographic device based on nitrocellulose membrane. Combining the device with SELEX screening technology, the authors obtained a high concentration of the outer membrane protein (Omp) of Salmonella typhimurium after 7 rounds of positive screening and 3 rounds of reverse screening. Affinity aptamer 33 and 45 sequences with a detection limit of 10-40 cfu/mL. Gel column is also a common immobilization medium for aptamer screening.
Tang et al. used SELEX technology to screen aptamers with high specificity and high affinity for red bean toxin based on agarose gel resin, and their dissociation constants were as low as several nmol. In recent years, the use of microwell plates as immobilization media to screen aptamers has been widely used. Wang Lifeng et al. screened and obtained a high-affinity aptamer sequence that can specifically bind to carcinoembryonic antigen (CEA) based on microwell plate technology. Studies have shown that this aptamer plays an important role in the early diagnosis, monitoring and treatment of tumors.
Fluorescent magnetic beads SELEX (FluMag-SELEX) technology. FluMag-SELEX is a method for screening fluorescent aptamers by combining the application of magnetic beads and SELEX technology. This method requires a small amount of targets and can directly measure the amount of binding ligands through fluorescence. Kim et al. applied FluMag-SELEX technology to screen five specific aptamers of ibuprofen, a broad-spectrum anti-inflammatory drug. Three of them are specific aptamers for racemic ibuprofen, and the other two can specifically interact with racemic and meso ibuprofen. The ibuprofen aptamer has strong specificity and has no binding effect with ibuprofen analogues and oxytetracycline. Xu et al. used FluMag-SELEX technology to screen specific aptamers for PCBs, with dissociation constants at the micromolar level and good linearity in the range of 0.1 to 100 ng/mL.
Capillary Electrophoresis SELEX Technology (CE-SELEX). There is a certain difference in the charge-to-mass ratio between different components, resulting in a difference in the electrophoretic mobility of the substance, thereby achieving the separation of different components. CE-SELEX can realize the screening of high-affinity aptamers within 2-4 rounds, and is often used to screen macromolecular substances such as proteins, lipopolysaccharides, and polypeptides. For the first time, Yang et al. used CE-SELEX to screen the aptamers of the small molecule substance methylmorpholine. After three rounds of screening, eight high-affinity aptamers were obtained, with dissociation constants ranging from several hundred nM to several uM, two of the sequences can catalyze the metal insertion reaction of mesoporphyrin, and the catalytic strength is 1.7 times and 2 times, respectively. Aiming at the problem of dynamic dissociation equilibrium between aptamers with weaker affinity and target systems, researchers have developed non-equilibrium capillary electrophoresis (NECEEM) of equilibrium mixtures, equilibrium capillary electrophoresis of equilibrium mixtures (ECEEM) and Non-SELEX capillary electrophoresis Electrophoresis technology.
Ashley et al. used Non-SELEX technology to screen the aptamers of catalase. The authors characterized their affinity and specificity by using a fluorescence spectrometer and capillary affinity electrophoresis. The affinity of the protein is as high as 100 times, which also shows its high specificity. The aptamer can be applied to biosensor, immunoblotting and biomarker identification. Ashley et al. screened the aptamers of human leptin protein in a free three-dimensional space environment based on NECEEM and SELEX technology, and its dissociation constant was several hundred nM.
Cell SELEX (Cell-SELEX) technology. The main target substances of this method are cells, bacteria or viruses, etc. During the operation, centrifugation and precipitation methods are used to separate and remove unbound suitable ligands, and then specific aptamer sequences are obtained through thermal dissociation or enzyme digestion.
Liang et al. used Cell-SELEX to obtain 5 DNA aptamers after 35 rounds of repeated screening for living cells infected with rabies virus. Virus titer determination and real-time quantitative reverse transcription PCR experiments showed that the five aptamers screened could inhibit the replication of rabies virus, which provided a possibility for the treatment of rabies infection.
Ninomiya et al. used Cell-SELEX to obtain 12 high-affinity aptamers that specifically bind to human liver cancer cell HepG2 after 11 rounds of repeated screening, with dissociation constants ranging from 19-450 nM. The author analyzed and obtained the secondary structure shared by 12 aptamers, which is closely related to the recognition of HepG2. This method can be screened when the nature of the target is unclear and the binding site is not determined, and it does not require a complicated process for preparing the target.tamer screening of cells, bacteria and viruses, and solid-phase adsorption and elution technology can be used for aptamer screening of soluble small molecules.
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I want to screen for antimicrobial activities of spices extract against fungal isolates from food. what method /methods is best for it?
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There are plenty of relevant articles on Google Scholar - e.g. https://academicjournals.org/journal/AJMR/article-full-text-pdf/15879FE11467.pdf
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For example QTL (x) is identified for disease screening in the mapping population where the parents are A and B. Can this QTL (x) be used as marker to screen the another population where its parents are C and D for the disease resistance?
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Keep in mind that a QTL is detected specifically when the offspring are segregating for different alleles of a gene affecting the trait. E.g. if parents A and B are fixed for functionally different alleles, their F2 offspring will segregate for AA, AB and BB combinations of alleles at a QTL with different degrees of disease resistance. When you cross parents C and D, of both parents are homozygous for a resistance allele or if both are lacking a resistance allele, there will be no QTL effect.
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E coli strain JM109(DE3) has its genotype listed as endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ Δ(lac-proAB) e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+) (λDE3).
It seems like it should be suitable for blue-white screening because of the lacZΔM15 deletion and but Promega states it isn't suitable. Another supplier, IntactGenomics says it is good for blue-white screening.
Anyone have an explanation? Is it just that it's an expression strain and not a cloning strain?
Thanks!
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I agree with Robert Adolf Brinzer that JM109 is (in theory) suitable for blue white screening. I have used the non-DE3 version often in the past. However it can lose the F' which carries the lacIq lacZΔM15 fairly easily so you may wish to steak the strain on minimal medium without any amino acids. The F' is maintained because it carries the proAB genes to complement the proAB deletion on the chromosome.
Another issue is that while you might need to add IPTG to really produce good color, in doing so you are doing to induce T7 polymerase expression from the DE3 prophage. So this may actually counter select against the colonies you want if you are cloning into a T7 expression system. So be cautious that you may be specifically selecting against the clones you want. That is probably why Promega says to not use it that way.
You would be better off to do the initial blue-white screening in a non-DE3 strain and then when you find the correct plasmid to retransform the DNA. However if you don't have any non-DE3 strains then you might try with just X-gal and no IPTG and you might still be able to see blue/white but more faintly.
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Antibiotic resistance , enzyme production are types of screening method . Both have advantages and disadvantages so , in these two which method is more preferred to insert in vector and provides maximum results . Are there any considerations of these methods while performing gene cloning?
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Hi there,
Your question is a bit of unclear to me... When you mention enzyme production, do you mean the enzyme produced by the gene conferring resistance or do you mean the product of the gene of interest possibly cloned into the vector?
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I am conducting a systematic review and after screening have come up with 9 included studies 6 of which are systematic reviews. Am I able to use these in my report and discussion? As the articles the reviews utilise / mention are outside of our date range?
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No,it is already shows polled effect
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In the REDIAL project funded by Kidney research UK We are looking for a dynamic and talented early career researcher in the field of Computational Materials Science with a focus on Separation and Biomedical applications, who can effectively exploit Artificial Intelligence tools for the design of functional Materials and Sustainable Processes in the SusProM Group. https://www.suspromgroup.eng.ed.ac.uk/
The Opportunity:
  • To work in a highly interdisciplinary Research Team with Chemical Engineers, Bioengineers an Experts in Artificial Intelligence and undertake research on the computational simulation and screening of materials for the miniaturisation and circularisation of the hemodialysis process.
  • To exploit the research results to improve the life of kidney patients, in collaboration with Kidney Research UK and frequent contacts with patients, nephrologists and biologists during National events.
  • To deal mostly with computational work, but willing to carry out experimental validation in the lab if needed.
Your skills and attributes for success:
  • Computational material science skills (Molecular Simulations, Macroscopic models and Machine Learning)
  • Strong fundamental knowledge of materials for separation theory
  • Ability to apply fundamental skills to problem solving
  • Coding skills (Python preferred, Pytorch/tensorflow desirable)
  • Basic experience in the lab.
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when will you be submitting your PhD?
you can apply
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I wanna use it in TSA medium to screen some characteristics of bacteria. I used 0.25g PbCl2 with 0.3ml HCl 12M, but this did not dissolve PbCl2
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as you see, it's insoluble
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Metabolomics has emerged as an invaluable tool for prognostic and diagnostic purposes, last in the cascade of others OMICS -genomics, transcriptomics, and proteomics. Omics training usually covers experiment design, data generation, and collection, data preparation, data analysis, and the last but not the least - data interpretation.
At the end of this meticulous energy, time, and financial-consuming path, it might be totally none sense to fail to put your results into the broader biological context.
For those like me that have never been trained to interpret metabolomics data, how can we make sure to not miss important points? Is Basic knowledge in Biochemistry, Physiology, or physiopathology of the disease of your interest, enough to harness the full potential of metabolomics technologies for biomarker screening u.a?
I would like to discuss with experts out there, the most important assets for a right and successful data interpretation of metabolomics data.
Thank you for sharing your experience in the Metabolomics journey as well.
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Only advice I can give is to have a background in chemistry so you can identify what chemical groups make sense and to be able to investigate related chemical families or to predict parent compounds. For example there may very little on a glycoside but if you look up the parent compound as an alcohol or methoxy ether you can often find a trove of relevant literature. Read all the papers you can about the annotation for the compound as there can often be non-enzymatic routes to the formation of compounds (especially when ROS is involved). Look up other synonyms for the compound as these are not standardized, especially in older literature. Use KEGG to help visualize the pathways where possible and always doubt the automatic annotations. I have seen nature papers where their "marker" for a cancer type is actually a tropical plant alkaloid only produced in one family of trees from southeast Asia (the patient samples from that study were not even from that region of the world). Also if you think the metabolite is important you must always do an alternative assay to verify that it is the correct annotation.
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I just established a cell line after long-term term culturing and would like to screen for contamination. I only have Hoechst 33342, and I would like to know whether it can be used for mycoplasma screening.
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Hello Xi Su
Yes, you may use Hoechst 33342 for mycoplasma screening.
Hoechst 33342 is a cell permeable fluorescent compound that is able to stain the DNA of eukaryotic as well as prokaryotic cells by binding with high affinity to the minor groove of AT-rich DNA sequences. Both Hoechst 33258 and Hoechst 33342 are very closely related bis-benzimides dyes, and are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescence light around an emission maximum at 461 nm. The key difference between them is that the additional ethyl group of Hoechst 33342 renders it more lipophilic, and thus more able to cross intact cell membranes. Hoechst 33258 is significantly less permeant.
You may use Hoechst 33342 for mycoplasma screening. Please refer to the link below. See page 28.
Hoechst 33342 is commonly used as a DNA binding dye to determine cell cycle status and apoptosis assays.
Best.
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For example, if a biparental mapping population is screened for abiotic stress tolerance (drought/salt) with physiological parameters.
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Hi Nikolay, many thanks for your input. I highly appreciate your time and effort.
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Hello everyone
I have done simulation of 100 ns by using gromacs.. and the following is hbond plot but its different i dont understand that every thing is fine but the data formate in .xvg file is different, its look like mixed up .. here i am attaching two screen shots but if you want to check the .xvg file then i will send you
Please someone help me i dont know the solution
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i have use this command but the same problem
gmx hbond -s md_0_1.tpr -f md_0_1_center.xtc -n index.ndx -num hbond.xvg
i dont know what is the main problem
can you please help me to sort it out.
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Hello,
I'm looking to implement a fast method to screen anti-inflammatory products. Avoiding protein denaturation is linked to anti-inflammatory properties.
There is a nice number of papers describes a rapid and low cost screening method based on an incubation of the tests compound and a BSA solution (about 1 to 5% w:w) at higher temperature (usually 72°C) for a given time (between 5-20 min) buffered to ph6.4-6.6. Reading is done by spectrophotometry (usually 660nm). The method seems simple but when I try to implement, my reading is zero. I noticed that each laboratory introduces a slight change on the most cited protocol. I have introduced the major changes reported without any reading. Papers reporting the raw data indicates that after incubation the negative control (no test product of anti-inflammatory drug) DO is about 0.3-0.4.
Notice that all weightings, control and technicians have been investigated to try to find the root cause, unsuccessfully. Do anyone have any experience on this that can give me any advice?
Thanks
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Dear Adam,
Thanks for your comments but my DO reading is close to zero. No turbidity, no nothing a clear crystal solution.
Thanks again.
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Are preoperative (3D printed) models, and drug testing/ screening models classified as medical devices? What category of devices do they fall under?
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Dear Colleagues,
I wish to perform a genotype-phenotype correlation study in a family with a known mutation in RPGR-ORF15.
However, the RPGR-ORF15 region is very tricky because it contains GC repeats and thus its not possible to amplify with standard PCR conditions.
Special protocols are needed for both PCR amplification as well as during Sanger sequencing.
Therefore, I would greatly appreciate if anyone could suggest me a commercial facility where I can send my samples for screening the specific mutation located in the RPGR-ORF15 region?
Number of samples to be screened = 05
Many thanks,
Atta
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There are several commercial facilities that offer specialized services for PCR amplification and Sanger sequencing of difficult regions like the RPGR-ORF15. Here are a few options:
  1. GeneWiz: They offer Sanger sequencing services for difficult templates, including those with GC-rich regions. They also have a team of experts who can assist with assay design and optimization.
  2. Eurofins: They offer custom PCR and sequencing services for challenging templates, and have experience with GC-rich regions. They also have a team of molecular biologists who can assist with assay design.
  3. GENEWAVE: They offer PCR amplification and Sanger sequencing services for difficult templates, and have experience with GC-rich regions. They also offer customized solutions for specific projects.
It is recommended to contact these companies and discuss the specifics of your project with them to determine which one is the best fit for your needs.
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For example, when searching over the PubMed database, we get 125,000. But PubMed has some limitation that just allow us to screen until 1000 results. Do anyone come across this situation, and what you do to solve this issue, or anyway to report the results?
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PubMed allows you to export the first 10,000 results to a citation manager - click on the 'send to' button at the top of the search results. There are also other options to for export the results that may be more relevant to you.
However, you have to bear in mind the time it takes to screen the results. If you screen one result per minute, that is 420 results in a standard 7hr workday. Meaning to screen 125,000 would take 298 days. Obviously, this is not feasible.
Use more search terms, Boolean operators (AND, NOT, OR), and search filters to get your results to about 1000. That is reasonable for a systematic review.
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Hi everyone, I wanted to know if there is a method that allows us to screen bioactive molecules with protective power against UV rays. For example, exposing a support containing the molecules to UV rays, and then we observe directly (visually) a character that allows us to select the protective molecules. Thank you in advance
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Can I directly evaluate the effect of the organic extract (ethyl acetate) for its protective power against UV rays?
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Field screening of rice genotypes against brown plant hopper was done on the basis of hopper population/hill/week. The genotypes are to be categorized under different levels of resistance but there is slight confusion in choosing the standard scale for BPH evaluation under field conditions. Please help verify the two scales attached in JPG format or share any article or web link regarding my query.
Thank you
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Look this Rice-standart-evaluation-system from IRRI on 20 page. Hope it helps you.
The best regards Liane
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I am working on the degradation capacity of naphthol by 6 bacteria isolated from wastewater which are : Pseudomonas aeruginosa , Pseudomonas Putida , pseudomonas fluorescens , Bacillus thuringiensis , Citrobacter freundii and Escherichia coli but I want to know which is the best composition of the minimum medium for the screening of these bacteria .
Thank you in advance.
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Yes , for utilization of naphthol as a carbon source .
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Hi all,
I wonder whether there is a way to find the original natural product from the modified structure? It is very common to use natural product derivatives in HTS/HCS/in silicon screening to find more potent structures and/or SAR. Once, we have a candidate structure, is there any database where we can do search (based on structure similarity/substructure) and find the originated structure and the source (which plant, microorganism etc.)? Thanks
Best,
Burak
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Transformed bacterial screeening
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Thanks all!
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Hi everyone,
I am currently trying to screen the behavioral effects of the 6-OHDA unilateral injection in the striatum of Wistar male rats to confirm a Parkinson's animal model by apomorphine-induced turning behavior test (or rotameter test).
Although the dose of apomorphine is prepared according to the articles and is injected intraperitoneally to the rats, but instead of turning against the injection site of the neurotoxin, the rats become extremely relaxed and even fall asleep, and it is not possible for me to check the behavior.
I have request from dear students, professors and scientists that help me to find out what the main problem is and share the tips of rotameter test with me.
Please feel free to have contact with me.
Thank you for reading and helping!
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Please find the detailed protocol attached.
Thanks
Samir
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I have National Cancer Institute screening results of my compounds. Upon submission, I am asked by the reviewers to provide standard deviations, what kind of statistical analysis was performed, and its significance. I have scourged the NCI methodology section and many publications throughout the years and couldn't find anyone who met these criteria.
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Emmanuel Curis The main problem is that NCI doesn't provide individual data of each experiment. Instead, they provide you with the mean. for every cell line.
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I used 0.5% Aq. AcOH solution to dissolve chitosan and derivative to evaluate their antimicrobial activity and performed anti-bacterial activity and antifungal activity using the broth dilution technique in 96-well micro-trays (NCCLS, 1993) and the antifungal screening method (NCCLS M27-A2 protocol, respectively. The cytotoxicity tests of prepared chitosan derivatives were assessed by MTT assay.
Now reviewer have following comment.
kindly help me in replying the comments of reviewers
comment of reviewer: if the derivates are still not soluble in water how are they be used in the kind of treatment proposed? What it's the implication of using acidic media in antimicrobial or cytotoxic treatment ?
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The reviewer is apparently concerned that the solution is not useful for clinical application due to the acetic acid - that one can not for example inject acetic acid internally. You need to defend the safe use of acetic acid internally AND that chitosan remains solubilized when delivered to the tumor in the body.
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Hi all..
I am doing the in silico and in vitro anti-tuberculosis screening. Any one engaged in finding anti-tuberculosis compounds or related screening work. Hope someone engaged in the area can help me.
Thankyou in advance.
Shefin
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Dear Dr Kholis,
Yes it's possible
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I have a bit of a semantic question in relation to a paper I am writing.
Recently, I had a discussion with my supervisor on Type-II errors. In my recent statistic course, the type-II errror was defined as falsely not rejecting H0. A lack of power is often described as a cause of a type II error. As such, I was under the assumption that a type-II error is mostly used in the context of statistical tests.
However, my supervisor explained that "type-II error" could just as well be used describe any false negative result, also when it relates to methodological problems e.g. using the wrong measurement instrument for your aims and falsely not finding any effect.
I cannot find anything on pubmed on this matter. I screened some papers mentioning the Type-II error but all used it in a statistical context, not more general.
As such, my question is whether 'type-II error' only relates to a statistical problem or also to a broader definition of false negative outomes?
Thank you.
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A Type II error is a decision error made about the data. Additionally, there must be a hypothesis of some kind present. A measure, instrument, or procedure does not contain a hypothesis.
Perhaps your advisor is getting at something like this: If a study has a flawed methodology, this will affect the data, which could result in a Type II error (miss). So, one's methodology could indirectly lead to a Type II error, but the error itself is about the data - not the measure or procedure used to collect the data.
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any technical input for PCR confirmation
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Hpt confers hygromycin resistance so can be used as a marker gene. Use hygromycin selection and all surviving plants should be what you want. If you really need to PCR your plasmid genes as a positive control you can just use the plasmid sequence to design primers and then BLAST them against the genome of your plant.
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I have read various articles in which plastic degraders are screened using PEG-containing MSM media. But which PEG is used is not mentioned. So, I used PEG-6000 as the sole Carbon source to isolate the plastic degraders.
I want to get suggestions about the best solid medium for plastic degraders.
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Kindly review it will help you out.
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I need to screen my plant extract for all the compounds present in it. So i want to know which technique (without using standards) will give me the results
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At first, the objectives of the experiment, the type of secondary metabolite, the appropriate plant organ and the sample preparation processes should be considered and determined. Then, according to the available literature reviews, the effective isolation and identification techniques should be used to achieve the desired result.
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I would like to create peptidomemetics based on a certain known peptide in silico.
For this I am asking if there can be any good references on this topic. (in silico specifically)
What I Want to do is; to create a small library of these peptidomemetics, then to screen their ability to dock a certain active site, then to run a molecular dynamics simulation to check the stability of the complex and the affinity to each of them.
Potential inquiries are;
Which software to use for building?
Which software for docking?
Which software to get parameters for these peptidomemetic to run the Mds? Or is there a preferred forcefield for these compounds?
Or
Should I deal with them in the same way as small molecules (this can be an answer that reliefs me at the end)
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The Molecular Modeling Tool Kit (MMTK) is a user-friendly software package for building and simulating peptidomimetics. It includes tools for building and manipulating peptide structures, and it can be used to generate a variety of peptidomimetic structures, such as small molecules and peptidomimetics. The PyMOL Molecular Graphics System is a powerful open source software package for creating peptidomimetics. It includes tools for building and manipulating peptide structures as well as visualizing them. For docking, the AutoDock Vina software is a widely used open source program for protein-ligand docking. It is a powerful tool for predicting the binding affinity of peptidomimetics to the target protein.
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Is there any maximum number for studies that can be included in scoping review?
This is Homa Arshadi, Ph.D. Candidate of medical library and information sciences. I need some help with the following question. Could you please kindly advise me.
I'm doing a scoping review on text mining topic. The number of records identified after searching databases are about 10,000. After screening of 60 percent of these records, about 500 of papers are eligible, which must be read by full text. Is it reasonable to perform such review? What is the maximum number of studies to be included in a scoping review?
Many thanks in advance!
Kind Regards,
Homa
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Hi Homa, I don’t believe there is a recommended maximum of studies to be included in a scoping review (not that ive come across any way). You seem to be getting a large number of results so it could be worth revising your research question with you supervisor and/or search strategy with a librarian to narrow your focus (if you haven’t already). Id recommend Covidencr software. I found it useful for managing the data.