Science topics: PhysicsCondensed Matter PhysicsScintillation
Scintillation - Science topic
Explore the latest questions and answers in Scintillation, and find Scintillation experts.
Questions related to Scintillation
Hello All! I am working on a project that uses stable isotope analysis (C and N) to look at the diets of California reef fishes. I am going to be collecting Liver and White muscle tissue. It was suggested to me to use 20ml borosilicate glass scintillation vials (urea caps with polyurethane lined caps/not foil lined) for my tissues. I will be freezing the tissue samples in the vials and drying them in a 65C drying oven in them as well. The issue I am running into is that every brand of vials are back ordered for about 4 months no matter where I look.
So I wanted to see if 1. Anyone in the southern California/greater LA Area had vials I could buy off of them to use. Or 2. If anyone knew of a substitute I could use. It has been suggested that I could hand make aluminum foil packets, pre combust them, and store/dry the tissue in those. However, I would prefer the glass vials for both, organization/storage sake as well as I will eventual be grinding the powder into a powder and vials would be less likely to fail with the powder.
Thank you in advance for any advice!
I have for many years been measuring bacterial production using 3H-Thymidine and 14C-leucine. For these measurements I have used the Scintillation Cocktail “Ultima Gold”, but this cocktail has become rather expensive and I am therefore looking for an alternative.
Does anyone has experience using the “Cytoscint scintillation cocktail”? It is around half the price, but the question is, does it work ok?
Thanks for your help.
I'm using 0.1N NaOH because I am performing liquid scintillation counting to detect tritiated glutamate and I need to normalize. NaoH is compatible with the scintillation liquid but the light blue color I see with BCA makes me wonder if NaOH is compatible with the BCA reagents. Any thoughts or alternative lysis buffers would be appreciated.Thanks!
I'd like to find a lab where I can pay someone to determine the percentage of pollution in a biological sample based upon the amount of carbon 14 present in the sample. The approach was originally used by Ogawa. See attached paper.
As early as the 1960s, lithium glass scintillators have been developed for neutron detection, but now the main commercial scintillator materials are concentrated in scintillation crystals. Why did this phenomenon occur, and what caused this result? Compared with scintillation crystals, what is the difference between glass scintillators?
the experimental protocol for measuring the light output for a gamma scintillation detector
I was able to find data on vacuum ultra violet (VUV) light attenuation in gaseous and liquid argon (GAr, LAr) and in liquid xenon (LXe), but unfortunately not in gaseous xenon (GXe).
I would like to know two things:
1) Does an answer to the question exist? Or has there not (yet) been data published?
2) If there is data, could you please provide a reference to it?
Thanks a lot!
Ga2O3 has shown 3 direct allowed transitions of different energy in the a,b and c-axes. When measuring the bandgap with polarized light, each of these transitions can be probed so you can observe different bandgaps with a Tauc plot ~ 4.2-5.12 eV. My question is if these different direct-allowed transitions could produce different scintillation emissions from either photoluminescence or cathodoluminescence?
Used in radiation measurements
I am making a simulation of energy deposited of some radioactive sources in scintillation detector. I have some results, but I don't have the same distribution energy between experimental spectra and simulated spectra. I do not know why.
For instance, the spectra of Cs-137 is shown. However, it does not have the same resolution.
Someone who can help me how to improve the simulation.
The scintillation detector is of NaI:Tl.
We have analyses from vendors (XRF), onsite XRF, ICP-MS and ICP-LA. We've had problems with complete dissolution/digestion, and one wildly different set of data. These determinations are critical for bringing raw materials in through US customs. I'm years away from my studies of radiation, and am hoping experts will help me here. Could we use a scintillation detector and roughly calculate ppm content of Th232 and U238 ( at less than 1%)?
I intended to insert a sensitive photodiode instead of the photomultiplier in the nuclear scintillation detector.
selection criteria of scintillation materials suitable for FAST neutron detectors
I am working with a Septrino PolaRxs 5S GPS receiver which receives the data from GPS satellites and after some initial processing stores it in .ismr format (ISMR= Ionospheric Scintillation Monitoring Records).
Though I am able to open the data file in a notepad I don't exactly know how to read the data in Matlab. Is there any program or algorithm available in Matlab which can help me in reading the ismr files?
I am trying to simulate the electroluminescence process in noble gases (argon and xenon) through Geant4 but I haven't found any documentation on how to add this process to the physics list.
Is there a standard physics list that already has this process? Or a simple way by which I can define the secondary scintillation phenomena within the simulation?
When alpha particle interact with a scintillator materials such as ZnS(Ag), it generates electron hole pairs in the medium due to ionization. When these electrons combine at luminescent center it generate light.
My questions is whether these primary electrons are capable of generating secondary electrons or not? if yes how to quantify them and what is their contribution is radio-luminescence/ scintillation?
As we know that at high energy, the response of NaI(Tl) scintillator decreases due to less photo electric probability at high energy. I want to know what happens with plastic scintilltor at high energy?
Please suggest and attach some reference if possible.
Zugzwang is a German word which translates to "compulsion to move." There comes a time in chess, when moving your piece is fatal to your game, and, not-to-move is not an option that you might otherwise exercise.
Has the Gordian knot of data -- and the compulsion to add more data, sans reflection, sans a salient matrix, sans logic, sans common sense, sans clinical judgment, sans practically everything except unthinking tribute to mathematical numbers and statistics, in the pursuit of a puzzle wrapped in a mystery and placed inside an intellectual riddle besides a scintillating/shimmering mirage -- that is pathognomonic of migraine research, sealed the fate of migraine and its researchers both to zugzwang?
In medicine, and medical research, how you start determines where you will end!! Sometimes, you start in African safari, stare at huge turds, and end-up with your name linked to lymphomas for perpetuity--not in a laboratory.
Is it too late already? Will data indeed overwhelm logic and common sense quintessential to genius? Or will inspiration clear the deck and ring the bell?
After our building performed a switch from grid to generator power and back again, our liquid scintillation counter has been stuck "loading" (on machine display) for days. Have tried restarting, resetting, unplugging, checking software settings on the PC program (MultiCalc), switching floppy disks, etc. Nothing will make the machine un-freeze. Any advice would be appreciated!
Has anybody some experience doing this kind of experiments? We are trying to quantify glutamate uptake by human astrocytes using the method followed by Pines and Kanner in 1990, with tritiated glutamate.
We have some doubts regarding the methods used to quantify radiation. Is it better to use a scintillation counter or a microbeta? Does someone have a specific protocol to do so?
Thank you in advance!
The any one refer decay times of scintillators which are actually used in scintillation detectors?
Hi! Can anyone give me the composition and/or preparation protocol of xylol base Scintillation fluid?
1. Can Compton Scattering like scattering happens for K shell electron like it happens for valence shell electron for X and Gamma Ray?
2. If yes, probability is more for K or Valence Shell electron? And more importantly why its high (either K or valence shell)?
I am working with the system which included PMT, scintillation detector, Pre-amp, amp, and MCA. I get the gamma spectrum from MAESTRO software but i don't know how to analyze and calculate the efficiency of the detector. If you have any paper or references about this, Please send to me. Thank you so much.
This is one of the toughest questions in migraine pathophysiology.
I have described my own scintillating scotoma in the attached file.
I am currently in the process of submitting an exhaustive review on CSD following the commissioning of the article by Editor-in-Chief of Journal of Neuroscience, having performed an exhaustive search of the scientific literature.
To be homonymous, the SS must be binocular and distributed in both right or both left halves of the visual fields of both eyes. I have not found any such case of SS distributed in a binocular fashion.
I have also described the basis for retinal origin of SS in the attached article. No in vivo proof can ever be obtained of mechanically-triggered SS at the level of the retina.
Please, how do I convert intensity to counts in a NaI detector? For example, If I calculated Intensity of 6mR/hr at 1m, and I need to use the NaI detector to measure the same sample at 1m, the detector returns the reading as counts per minute. Please, how do I relate this to intensity? Convert from counts to the intensity and vice versa?
Can anyone help with technical information on the Co-60 calibration for a gamma spectrometer? (NaI detector)
Basically I have now two proteins that catalyze the same reaction, but with different affinity for their substrate. The first protein has a lower Km since is enough to visualize the reaction (using a scintillation counter) if I use a concentration around 10-50 nM. When I use the second protein I have to add at least 400-800 nM to be able to visualize the reaction and calculate the different slopes varying the substrate concentration to finally obtain both Km.
As far as I know the concentration of the protein is not relevant to calculate the Km (you only have use a concentration much lower than the substrate of the reaction in a Michaelis Menten-like case). Since my knowledge about enzymology is not very deep, I would like to hear of you that is perfectly possible to compare both Km even if I had to use different protein concentrations to perform the assay.
Thank you very much!
I'm currently optimizing my [35S]GTPγS assay in whole rat brain homogenates and I'm only getting 50% stimulation over basal with 10uM DAMGO. Another thing is that the difference in efficacy between DAMGO and morphine is rather small (148 vs. 125%). Thus, DAMGO doesn't seem to act as a full agonist in my system. When I was running the assay (in a 1 ml format) in another lab Emax values were 190% for DAMGO and 140% for morphine and I could clearly see the difference between the efficacy of both drugs.
My binding buffer composition is as follows: 50mM Tris-HCl (pH 7.4), 3 mM MgCl2, 100 mM NaCl, 1mM EGTA and 30uM GDP. The assay is run in a 250 ul format with 0.05 nM [35S]GTPγS and 15ug/ml of added protein for 1h on a shaker set to 30oC. The samples are filtered with the FilterMate Harvester onto GF/B Unifilter plates and dried for 2 h at 50oC. The scintillation fluid I'm using is Microscint-20 (45ul on each filter). I've already tried different protein, GDP and Mg2+ concentrations with no improvement in efficacy. I'll be running another experiment with different Na+ concentrations next week, but I doubt that'll bring a breakthrough though. Did anyone of you encounter such a problem with your assay? If you have some tips please share.
We use the liquid scintillation detector with Si detector to detection UV radiation (generate from laser), this source is a vary the input power and number of. Pulses. The shape of pulse (rise time) is related with this two parameters. Expectation the increase of input optical power is lead to decay of the rise time and the increase of number of pulses no effective.
Hi, my question is
What is the best way to quantify radiolabeled RNA?
I have a plan to T7 run off transcription and 5' end labeling. After that how can I determine molarity? I thought about using beckman scintillation counter check radioactivity and back calculate RNA concentration. And I also found I can use phosphorimager to quantify my radiolabeled RNA. What will be the best way to quantify my radiolabeled RNA?
Please share any idea. Thank you.
I have never worked with this technique and need help with some of the basics. If anyone known to this field has any information I can follow up, I would really appreciate it!
The experiment involves Enzyme X which was incubated with a substrate solution containing a known concentration of Phospholipid and Cholesterol. 180 000 Counts/min 3H labelled cholesterol was added to trace the reaction . Aliquots were collected every 2 min for 10 min. TLC coupled with scintillation detection was used to extract and analyse the lipid content. Cholesterol and Cholesteryl ester were seperated and quantified using HPLC-UV.
I want to know how to calculate the basics such as % Recovery, % Cholesterol conversion and Concentration of the ester in mM, given the different times, and for each time the analytes (Cholesterol and Cholesteryl ester), their 3H counts and the total count.
As well as how to calculate the enzyme activity of enzyme X in terms of Cholesterol ester produced.
If anyone could share any links to articles/books which explain the above I would be greatful!
I am doing experiments where I am feeding Arabidopsis plants with radioactive phosphate (33P) to measure rate of Pi uptake. We will measure the uptake by scintillation counter which will give the data in counts per minute. How to convert counts per minute data into nanomoles phosphate per mg of plant tissues? Please let me know. Thanks.
Climate change issues are baffling the growing uncertainities of agriculture worldover, with aridity and salinization as two premier issues to address at. There are still many issues, we have yet not made the desired inroads in terms of raising the sustainability of arid agriculture . In this background , i propose following set of questions to our learned colleagues for your scintillating responses as usual:
* How are salinity and aridity related to each other?
* How are crops or cropping sequence selected in arid zone ?
* Whether or not , such selection criteria differ from annual crops to perennial crops?
* What are the set of soil suitability criteria frequently used for annual crops versus perennial crops?
* How are crop management strategies different from annual crops to perennial crops?
* What are the performance indicators of arid agriculture ?
Does anyone know that : How to measure the decay time of scintillator material? I would like to measure the decay time and afterglow of scintillators which are used for X-ray detection (particularly with synchrotron source).
Why do electrons produce more scintillation light than heavy charged particles?
I have a Bicron BaF2 detector (model number- 1M.5BaF2/2) lying around in my lab. There is no manual with it and Bicron, I suppose, is closed now.
I want to know if anybody has any information on what type of PMT this detector has?
I want to fire up the detector and its base says- negative voltage. We do timing spectroscopy and its timing is horrible. So, I want to change the voltage divider base, as thats the easiest thing to do. The scintillator and PMT are hermetically sealed with some kind of epoxy/soft rubbery material.
By the looks of the PMT pin - 20 pins- it seems to be a XP 2020Q.
Is there a way you can confirm this, please? I can always put a VD 124KT type base and see how it performs.
Does anyone know that : How to calculate or measure stopping power of scintillator material? I expect to obtain a percentage vs thickness result for a certain keV energy.
I don't know whether I can calculate it by using bethe equation
This number is very low but is not reported in literature. It depends on the system, obviously, but an order of magnitude in at least one case could be useful.
I have a pair of semi-annular NaI detectors for flow gas in pipe,but,the energy resolution is terrible.Does anyone know how energy resolution affected by shape？
Or.anyone give some advice to slove the problem.The spectrum for the annular detector with the source Co-60 has no peaks.Who can explain this?
Any suggestions will be appreciated.
I want find out the concentration of metal ion in organic phase as well as in aqueous phase in extraction studies.
I'm using the Quantulus Liquid Scintillation Spectrometer with the Perkin Elmer Filter Count scintillation cocktail in a plastic vial to measure the activity of Am243 and Am241 (separately) in two different conditions: radionuclide dissolved in an homogeneous solution and radionuclide deposited on a borosilicate glass filter immersed into the scintillation cocktail into the vial.
When I measure the Am243's CPM (counts per minute) I always obtain a 180-200% efficiency (CPM/activity[Bq/min]). When I measure the Am241's CPM, instead, I have an averaged efficiency of 86% in the homogeneous solution and an averaged efficiency of 120% with the radionuclide deposited on a borosilicate glass filter.
With respect to the Am243 I know that the Am243 (100 % alpha emitter - Qalfa=5.438 MeV - Half life= 7370 years) decays in Np239 (100% beta emitter - Qbeta=722 keV - Half life=2.3days) so I thought that the 200% efficiency was due to the secular equilibrium of the Np239 even if the Quantulus should discriminate the beta emissions and the alpha emissions. The problem is that the instrument doesn't show the presence of Np239 in the scintillation spectrum. Is this consideration acceptable in your opinion?
Regarding the Am241(alpha emitter - Qalfa=5.958 MeV - Half life= 432 years), it decays (alpha) in Np237 (100 % alpha emitter - Qalfa=5.438 MeV - Half life= 2.144*10^6 years) that is in transient equilibrium with the parent element. So I think that the presence of Np237 in the scintillation spectrum is negligible. In this case I can't explain the averaged efficiency of 86% in the homogeneous solution and the averaged efficiency of 120% with the radionuclide deposited on a borosilicate glass filter. Could anyone help me with this?
I have access to a Panalytical MPD materials Research diffractometer with a scintillation detector, a Euler cradle and divergent optics in the incident path, but I need help with the way to perform the analysis, how to position the sample height, how to optimize the difference angles, Phi, Psi...
In a first look, both are fluorescence, giving immediate light emission on irradiation. Mechanism wise both are similar. If both are same then why different terminology? Or is there any technical difference?
I want to replace a normal scintillation counter with a high-speed and high-sensitivity semiconductor X-ray detector in my Rigaku Ultima IV powder XRD system. One possibility I found is D/teX-25 from Rigaku. Are there better systems that are compatible with the Rigaku Ultima IV XRD available?
Especially for activators used in scintillation materials.
A factor of 40 boost of BaF2 scintillation light yield after electron radiation was reported in this paper. The experiment was done long time along. Is this observation confirmed by anyone else?
Until recently Whatman sold white UNIFILTER 0.45um polypropylene filter plates (cat no 7700-3305), however these plates have been discontinued and I am looking for alternatives. We use them in an assay where we trap precipitated radiolabeled compounds on the filter, then add scintillation fluid and use the filter plate for scintillation counting. There are a lot of filter plates available from different suppliers however we are specifically looking for white plates to minimize “cross-talk” in the scintillation counting process. Any suggestions?
Is there anyone here who is familiar with calculation of enzyme activity using a radio-enzymatic assay? I am working on enzymes inhibitory activity and I am planning to use radioisotopes. I am getting count per five minutes data in the end after counting the product form by a liquid scintillation counter. Any biochem expert here. need help
This is the link: http://www.ortec-online.com/download/Compton-Scattering.pdf.
I have tried so far, but it is difficult in the coincidence of Plastic scintillator and NaI scintillator. I use a 5mCi 137Cs and the diameter of the collimator is just 2mm. Is this diameter too small? If anyone has already done this experiment successfully, please give me some suggestions for the diameter of collimator.
Please suggest papers if there are any or your views on it.
Recently, we developed new scintillator materials based on bulk nanostructured materials. What other materials do you know of this type?
The conventional materials perform downconversion of 1 photon from a shorter to a longer wavelength. There are some materials that upconvert IR light to UV, on the basis of two or more photon absorption. However, I am interested as to whether there is material that can absorb blue photons (450nm) and emits UVC photons (280 nm)?