Science topic

Salts - Science topic

Substances produced from the reaction between acids and bases; compounds consisting of a metal (positive) and nonmetal (negative) radical. (Grant & Hackh's Chemical Dictionary, 5th ed)
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I am interrsested these days in the extraction of variable salts from Rejecter water and EOR , second stage waste water.
I hope, I find a feasibility studdy of this project and how much it will cost
the second quistion is about Lithium and Bormine extraction from EOR waste water, how mglL concintration is required to them?
thX #Oil #Wastewater #wastemanagement #EnhancedOilRecovery
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YES
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Is a[100] dislocation equal to a[010] and a[001] dislocation in rock salt crystal?
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I assume that you are referring to the Burgers vector as being either [100], [010], or [001]. Because rock salt has cubic symmetry, <100> directions are equivalent in that structure.
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Do you confirm this is a salt or protein crystal? i have sodium chloride 80 mM, Tris 25mM (pH7.6) in buffer. So i wonder if it is protein crystal or sodium chloride crystal.
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So you need to define what is happening here.
Normally, when you talk about buffer, this is the solution your protein was in prior to setting up trays.
Crystallisation condition is a better term, or mother liqour.
80mM NaCl is low, assuming your protein buffer is lowish NaCl too (~125mm), this is overall a low NaCl concentration. From experience, it would be unlikely that the crystal are salt, but it us always possible.
Whilst the Izzit dye is good, if you have limited crystals it's always better just to mount and shoot one without cryo to establish if these are salt as the diffraction will tell you straight away.
With the other crystals in the well, use a variety of cryoprotectants and shoot those, to find out which is the best.
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I am trying to synthesize silver nanoparticles from aloe vera gel extract prepared by cold extraction method of equal ratio of gel and water and i have prepared silver salt stock solution of 50ml. can anyone expert tell me which concentration of silver salt from stock and gel extract and distill water should i use for making silver nanoparticles. it will be very helpful.
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Take the concentrations, silver salts, which we took in our articles. The concentration of reducing agents in your gel is unknown. Therefore, you will have to select and check by UV-visible spectrum.
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Salinity intrusion is a major problem affecting the agricultural activities and posing health risk to coastal communities. There has been numerous studies highlighting its negative impacts both at national and global scale. Studies also exist regarding the mapping and accurately detecting salt water intruded areas in historical period. But, can the salinity intrusion be modelled with time?
Any insightful suggestions and techniques to do this from the vast research community of this platform will be highly appreciated!
Thanks!
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Krasimir Hristov Trendafilov thank you for your detailed answer. I appreciate it.
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My question is if I had a 10 kg soil in a pot. How much NaCl is to be added in the soil to maintain an EC of 5 dSm-1?
How many times we need to add the salt? to maintain the stress level?
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I suspect it's dependent on the type of the soils because they are complicated and various substances. Some ions would be adsorbed to be insolubilised upon specific soils. The general answer for this question would be DO experiments: adding several conditions of amount of NaCl to the subjective soil and measure the EC and decide the suitable NaCl amount.
If I dare to say, Na is less likely to be adsorbed. Thus, when you prepare the soil at 5 dSm-1 of EC NaCl, first dehydrate and totally dry out the soil (maybe using an oven), and then add the liquid media or water containing NaCl at 5 dSm-1 of EC to the dessicated soil. The required amount of media is dependent on your purpose. Evaporation of water will increase the NaCl concentration so you have to water with DW and maintain the total weight unchanged, or drain well and flow through the pot with the media of the exact EC level.
If the EC level has to be accurately controlled, the usage of sand as soil would be recommended, or water culture would be more suitable.
I'm sorry my answer is no so much simple but it would help your project.
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Hi, so i found out that there is a crystal formation on my triethylamine bottle. The crystals are light and will fall off the bottle with slight wind. The bottle are pretty much sealed im not really sure whats happening. All i can think is that someone in the lab recently changed the bottle storage from the solvent cabinet to the acid cabinet. Ive read somewhere on reddit that triethylamine cannot be stored together with acid as it can induce some kind of salt formation manifested as crystals. Ive wiped the salt and change its storage place however i wonder what is the salt, is it dangerous? Can i still use the solvent or no.
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I hope you're doing well! I wanted to touch base regarding the triethylamine storage situation. As you Fatin Safiudin know, triethylamine is a strong organic base, and keeping it next to acids is definitely not the best practice. It’s hygroscopic and can react with acidic vapors, especially in confined spaces, leading to the formation of triethylammonium salts on the bottle's exterior. The crystals you’re seeing are likely triethylammonium chloride if it’s near hydrochloric acid vapors, or a similar salt depending on the acid present.
First off, those crystals are generally benign, but it’s a good idea to clean them off to prevent any contamination. If you’ve moved the bottle back to the correct storage away from acids and wiped off the crystals, you’re on the right track! Just remember to use proper ventilation and gloves when handling it to avoid any skin or respiratory irritation.
As for the solvent itself, if the bottle is well-sealed, it should be safe to use. However, keep an eye out for any changes in odor, color, or clarity. If you Fatin Safiudin notice anything unusual, it might be best to discard it to avoid any internal contamination that could affect your reactions.
In short, clean it up, store it properly, and monitor the bottle’s integrity. That should keep everything in check!
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Hi all,
I am trying to identify a diatom that is fairly common in salt marsh samples from coastal Oregon (USA). I believe it is a species of Parlibellus, but I am unable to find a reference to it. It's typically about 40-48 µm long, and 8-10 µm wide. Striae are fine, 18-20 ITM, and more widely spaced at the middle. The central raphe endings are moderately distant and slightly deflected, and the central area is consistently oval shaped.
Thanks --
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Dear Sir, I am reaching out to other diatom experts on Research Gate to give me suggestions. I have already searched all of the available descriptions of Parlibellus that I have been able to find, descriptions that could be appropriate to the region. No luck so far. CALLING ALL DIATOMISTS! Any suggestions?
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Hi All,
I am currently using GROMACS to simulate high salt concentrations but I am running into an issue with gmx genion. If I have a 30x30x30nm box and want to use -conc to bring it to say 4M, then I encounter the error: Not enough replaceable solvent molecules! Any thoughts or adivice are greatly appreciated. Thank you.
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Adding an "-rmin xx " to the genion command also works.
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I am looking for proven scientific methods (or any national/International rules and guidelines) that explicitly mentions treatment / disposal of dewatered sludge residues / salts from Industrial wastewater
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The disposal and treatment of dewatered sludge residues and salts from industrial wastewater are governed by various scientific methods and regulatory frameworks to ensure environmental safety and compliance. Here are some key approaches and guidelines:
1. **Treatment Methods**:
- **Incineration**: This method involves burning the dewatered sludge to reduce its volume. The process converts organic materials to gases and leaves behind inorganic ash. This is particularly favored due to the increasing difficulty in finding landfill sites【36†source】.
- **Land Application**: Treated sludge can be used as a soil amendment, but this requires thorough analysis to ensure it is free from toxic compounds and heavy metals. The suitability of this method depends on soil characteristics, crop type, and local regulations【36†source】【37†source】.
- **Landfilling**: This remains a common disposal method, but it requires careful management to prevent groundwater contamination. Many regulations mandate the use of impermeable liners and leachate collection systems in landfills【36†source】.
2. **Regulatory Frameworks**:
- In the **United States**, the **Resource Conservation and Recovery Act (RCRA)** and the **Clean Water Act (CWA)** provide overarching guidelines for waste management, including the treatment and disposal of sludge【36†source】. The **EPA** has specific regulations under **40 CFR Part 503**, which set standards for the use and disposal of sewage sludge (biosolids), addressing pollutant limits and pathogen reduction【37†source】.
- Various states have additional regulations. For example, California's **Water Quality Order No. 2004-12-DWQ** builds on federal standards but also includes state-specific requirements for biosolid reuse【37†source】.
3. **Best Practices**:
- **Dewatering Technologies**: Techniques like filter presses and drying beds are used to reduce the water content of sludge before disposal【36†source】. The choice of technology can affect the subsequent treatment or disposal options available.
- **Reclamation Opportunities**: Evaluating sludge for potential recovery of materials (like metals or energy) can reduce disposal needs and enhance sustainability【36†source】.
For detailed guidance on specific disposal methods and regulatory requirements, you may refer to comprehensive resources such as the **EPA's guidelines** and regional water quality control boards, which provide localized information tailored to different states and municipalities【37†source】.
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The GTP that I am using is trisodium salt obtained from sigma. To prepare the GTP solution, I am dissolving it in 50mM Tris, pH adjusted to 7.5. The solution is sterile filtered, aliquotted and flash freezzed.
Please do confirm if it is a proper way of preparing GTP solution and If not then what is the better way or any other recommendation. I checked the phosphate level of water as well as buffer used for the assay. The value comes less than 0.2, which according to protocol is ok.
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620nm.
Another problem that I am seeing is the green color intensity keeps on increasing though it should not as the malachite dye itself should inactivate the enzyme
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Researchers have performed different corrosion tests such as potentiodynamic, electrochemical impedance spectroscopy, immersion and salt spray to explore the corrosion rate.
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Dear Mr Rahul Patil
In addition to the methods you mentioned, the Gravimetric (Weight Loss) Test method can also be used. This test method measures how much weight loss the corrosion causes on the material over a certain period of time. It is a simple and effective method to understand the long-term effects of corrosion on Al-Si based composites. The weight loss of the material can be measured by corroding the samples for a certain period of time and then cleaning the corrosion products.
I wish you good work.
Kind Regards.
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Hi everyone,
I noticed that there are different LB broth (lennox, miller) which are different in their sodium chloride concentration. As I am working with E. coli strain DH5α, will the choice of a higher or lower salt concentration (lennox vs miller) impact the yield of my experiment? thank you.
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Hi, DH5alpha is not affected by salt concentration, so either formulation is fine.
From my experience, the low salt LB is used for work with antibiotics zeocin, which is salt-sensitive. Of course you could have also some osmotic-sensitive strains of E. coli, but that's not the case of DH5alpha.
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Most attributes are used to delineate the external boundaries of salt. I know that the low coherence and amplitude and chaotic nature of salt make it difficult for seismic to study the inside. Most research is also dedicated to marking the external boundaries. Any ideas?
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The shear dipole sonic log can measure compressional and shear wave values as well as Stoneley slowness that can see approximately 150-250 beyond an open-hole wellbore. A Stoneley wave is a boundary wave that can propagate along a solid-solid interface. Hence, they can be used to identify salt fractures that might intersect a wellbore or be in proximity to a wellbore.
Near-wellbore features can also be interpreted using enhanced data-processing methods. Refer to the following paper "Processing acoustic logging data to image near‐borehole geological structures" X. M. Tang, Y. Zheng, D. Patterson
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1. I am trying to separate quarternery ammonium salt which is forming during the workup of my product, the product has high solubility in water. Tried extraction with different solvents & water combinations but does not help much, the product along with the salt is coming to the water part.
2. The salt formation could have been avoided in the first place if extra triethylamine (TEA) had been removed from the reaction mixture without the addition of acid, which resulted in salt formation.
Already tried alternatives such as
a. Kept under high vacuum at 60 deg C as the boiling point of TEA is 89 deg C
b. formed different azeotropes but TEA remains.
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I would attempt to neutralize the liquid to slightly >7.0 pH followed by a solvent extraction. Choice of solvent would depend on the organic species. If successful, step "a" from your previous work.
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What are the main causes of salts in groundwater?
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Hello! Groundwater salinity can be geogenic or anthropogenic in origin. Perhaps the following review article might be of some assistance to you:
The Anthropogenic Salt Cycle (KAUSHAL et al., 2023)
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Hi. I am doing a protein that has hydrophobic interactions with itself. I want to test the effects of a peptide which is a truncated form of this protein. That means I need first to open the interactions of these proteins protein (dimer) while adding the peptide, then recover the hydrophobic interactions to check if the peptide can bind to the protein. I already check pH, salt, and temperature. None of them works. Does anybody know any good detergents and methods to achieve this? Thanks!!
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You need to click the consultant's last name, otherwise the robot does not send a letter to the consultant.
If you have two identical proteins interacting and you replace one of them with a shorter one, then you do not block the hydrophobic interaction, but only reduce the negative Gibbs energy of this interaction.
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I want to calculate the electrical conductivity of Polyaniline (ES) powder and I want to know the best way to get the best results
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The electrical conductivity of polyaniline salt (ES) nanoparticles can be calculated using DC conductivity measurements by Ohmic Al electrodes at temperatures ranging from 303-423 K. Electrical characteristic study by using the two-probe manner, the samples had ohmic plots in which high linear coefficients.
FT-IR spectra can be used to determine the conductivity level and measuring methods of polyaniline emeraldine salt (PANI-ES) samples, the changes taking place were follow-up by the following measurements: FESEM, FT-IR, I-V character.
Field scanning electron microscopy revealed significant differences among the samples in terms of morphology. Morphology of polymer polyaniline by FESEM analyzing showed that the particle size of polymer is inside the micro-scale with the existence of acid. The activation energy of doped polymer PANi-ES has been deduced using the expression Arrhenius Equation, (Ea) is the energy level that the reactant molecules must overcome before an interaction can happen.
The electrical conductivity of polyaniline salt (ES) nanoparticles is measured by the molar ratio of DBSA to aniline. In an acid solution as DBSA under the influence of irradiation, the product will be in an Emeraldine Salt (ES) form of PANI, which pushes the reaction forward, with the presence of DBSA in the feed this could cause over oxidized which leads to form PANI nanoparticles in its Emeraldine Salt (ES) form, which is the conductive form of PANI.
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My teammates and I measured the conductivity of salt over time and with those results we determined TDS of what we think is NaCl as a product (ions Na+ and Cl-) using TDS=conductivity * 0.64 in which 0.64 is the conversion factor but we don't know how to find the initial concentracion of NaCl. We know the volume of water, the conductivity, the amount of salt added, density and the total time of dissolution. Should we consider that same TDS as the initial concentration? If say so, NaCl as a reactant shouldn't be decreasing over time?
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And how can we find the concentrations of both ions Na+ and Cl- as separate products? Because at some point if we consider TDS as those concentrations of ions, the initial concentration we obtained using the method you recommend us is lower than what we have in TDS. For example, we obtained 47.67 mol NaCl/m^3 as our initial concentration but at minute 3 we already have 51.46 mol Cl-/m^3, like can we have values that are greater than the initial concentration?
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Hi there!
Im working with hydrogels of agave lignin crosslinked with PEGDGE in alkali media but i need to wash them to quit the excess of reagents, when i wash them on water they swell and lose its structure, i tried on acid but the salt formation seems to be dehydrating the gels causing hornification, in which media could i wash my materials?
Thank you!
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I dissolved the 5 Kg of sample in 40 liters of water and left it for 24 hours. Then I used a pump to collect the supernatant liquid without disturbing the sediment and then filtered it for any impurities.
I repeatedly evaporated half of the water(40->20->10->5) and decanted the solution after sedimentation with the help of a transfer pump four times. After that, with further evaporation followed by sedimentation, some grainy crystals of salt(table salt size) were formed, and the supernatant liquid was removed with the help of a pipette (filtration could not be done due to the viscosity of the solution). I found that this salt was KCl and it was present in good amount in the solution (though there could be other salts too) which made the resin salty (but the resin tastes bitter, not salty). In the end, I evaporated most of the water and kept the solution for 12 hours, small grainy crystals(smaller than table salt size) of KCl formed in the solution, and the consistency of the solution was such that the solution could flow slowly. Then I centrifuged the solution for 10 minutes at 1200 rpm in a 50 ml tube and found that the viscosity of the solution was low at the top and increased up to 15ml mark in the tube, after that the semi-hard settled salt present in the bottom of the tube. but the taste of the solution is still salty.
Is there any method by which we can completely remove the salt from the resin ?
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Hey there,
As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions and basis sets to use for calculating the HOMO and LUMO. If possible, it would be incredibly helpful to receive links to papers or DOI numbers for further reference. Thank you very much for your valuable advice.
Regards,
Ilavarasan V
Project Fellow
Jain university
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Instead of reading the clever ones, I think you should have a look at the following link:
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After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a peptide; although: 1 amide bond). What would you recommend to either -preferably- generate the ‚free‘ zwitterion or to generate the HCl salt. Will diluting in 1M HCl suffice to protonate the TFacetate to evaporate it off and be left with the HCl salt?
Thanks!
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Another option, if hydrogenolysis conditions are compatible with your route, could be using a benzyl ester instead of a tert-butyl ester as protecting group. This way, you would be able to deprotect your carboxylic acid with H2 and Pd/C, and thus avoid the possibility of formation of TFA salts, as the isolation of your target compound upon completion of the hydrogenolysis should only require filtration on a Celite pad followed by the evaporation of your solvent.
I hope this helps,
Best regards
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Salts usually remain unmixed in the solutions, even after vigorous mixing, while preparing buffers for DNA isolation. Is there a method to it?
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Usually, to dissolve a salt, we need a particular pH. So, check the pH requirement for the salt you are using. By adjusting the pH, you can dissolve that particular salt. Additionally, some salts need a specific temperature to dissolve. After dissolution, you can store the solution at room temperature or the temperature required to store that particular solution. So, whenever you want to prepare a solution, you should first check its dissolution parameters. Then, you can start preparing the solution. Wish you all the best!
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Accrording to "Hydrolysis of Nylon, Bossert, Ohio Wesleyan University(1949)", we can degrade down Nylon 6,6 by hydrolysis using sulfuric acid. After hydrolysis reaction, adipic acid can be isolated by simply cooling down the temperature because it crystallizes well in acid solution. However, the free hexamethylenediamine is highly soluble in aqueous solution. So, this paper suggest recrystallization of hexamethylenediamine into dibenzoyl derivatives.
But, I want to isolate degraded-monomers (hexamethylenediamine) in its original form, not in the form of dibenzoyl derivatives. How can I isolate this basic organic compound from sulfate salt of hexamethylenediamine solution?
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Hi Lee, did found a solution for this problem, thank you
I work on the same reaction
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I need to do TEM analysis for my Polyaniline emeraldine salt form. May I know which solvent is appropriate for preparing TEM sample?
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Preparing Polyaniline Emeraldine Salt (PANI-ES) Sample for TEM Analysis
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I need to do TEM analysis for my Polyaniline emeraldine salt form. May I know which solvent is appropriate for preparing the TEM sample?
Answer
Preparing a Polyaniline Emeraldine Salt (PANI-ES) sample for TEM analysis involves several steps to ensure proper dispersion and sample quality. Below is a detailed guide on how to achieve this:
Materials Needed
  1. Polyaniline Emeraldine Salt (PANI-ES)
  2. Appropriate solvent (e.g., N-Methyl-2-pyrrolidone (NMP), Dimethyl sulfoxide (DMSO), or Methanol)
  3. Ultrasonic bath or probe sonicator
  4. TEM grids (e.g., carbon-coated copper grids)
  5. Pipette or micro-syringe
  6. Filter (optional, depending on solvent and particulate size)
Steps to Prepare the TEM Sample
  1. Choose an Appropriate Solvent: The solvent should be able to disperse PANI-ES effectively. Common solvents include:N-Methyl-2-pyrrolidone (NMP): Known for its strong solvating ability for conductive polymers. Dimethyl sulfoxide (DMSO): Another excellent solvent for PANI-ES. Methanol: Can be used but may not be as effective as NMP or DMSO for dispersion. Note: The choice of solvent might depend on the specific requirements of your analysis and availability.
  2. Prepare the PANI-ES Dispersion:Weigh a small amount of PANI-ES (typically a few milligrams, depending on the desired concentration). Add the solvent: Transfer the weighed PANI-ES into a clean container and add a small volume of the chosen solvent (a few milliliters). Sonicate the mixture: Use an ultrasonic bath or probe sonicator to disperse the PANI-ES in the solvent. Sonicate for about 30 minutes to ensure thorough dispersion.
  3. Prepare TEM Grids:Clean the TEM grids: If necessary, clean the TEM grids by rinsing with solvent and drying them using a gentle nitrogen flow. Drop-cast the dispersion: Using a pipette or micro-syringe, carefully drop a small amount (a few microliters) of the PANI-ES dispersion onto the carbon-coated side of the TEM grid. Dry the sample: Allow the solvent to evaporate at room temperature. This can be done in a clean, dust-free environment to prevent contamination.
  4. Optional Step - Filtration:If the dispersion contains large aggregates or particulate matter, you might need to filter it using a small pore-size filter (e.g., 0.2 µm) before drop-casting onto the TEM grid.
  5. Inspect the Sample:Once the sample is dry, inspect it under an optical microscope (if available) to ensure a uniform dispersion of PANI-ES on the grid.
  6. TEM Analysis:The prepared TEM grid is now ready for TEM analysis. Carefully load the grid into the TEM holder and proceed with the imaging.
Tips and Considerations
  • Concentration: Adjust the concentration of the PANI-ES dispersion according to the requirements of your TEM analysis. A very dilute solution might not deposit enough material, while a highly concentrated solution might result in aggregation.
  • Solvent Choice: The solvent should not react with PANI-ES or leave residues after evaporation that could interfere with TEM imaging.
  • Sonication Time: Be cautious with sonication time and power to avoid degrading the PANI-ES structure.
  • Storage: If the dispersion needs to be stored before use, ensure it is kept in a sealed container to prevent solvent evaporation and potential changes in dispersion quality.
By following these steps, you can prepare high-quality PANI-ES samples suitable for TEM analysis, enabling you to obtain clear and detailed images of your material.
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How to make buildings resistant to earthquakes?
Now in Iran, according to my suggestion, Unilit roof is used in the roofs of residential and office buildings, which is very light. I took this suggestion in an article for the seismological organization in Tehran and gave 14 suggestions to prevent the Tehran earthquake, including 2 They implemented it. One of them removed the bricks from the roof of residential and office buildings and put unilite and poured concrete on top of it, which is very resistant because there is a round rod inside the bits and it was mixed with concrete, and I also said that in metal buildings from 7 or 8 should be used next to the walls because it makes the Masguni houses stronger and also 2 parking spaces should be used under the buildings, like palm trees or dates, which have deep roots and will not fall during an earthquake. Buildings must have deep roots and also in the science of retrofitting structures, divergence is used, that is, natural or artificial rubber is used under the pillars of the houses, and steel springs are used in the middle, so that during an earthquake, the building, like a car or A car that has a spring and the springs play, the building goes up and down but does not fall, and this is a building engineering science that makes buildings resistant to earthquakes and natural disasters. And secondly, through the injection of water and salt solution, the energy of the faults can be removed. Because it comes from the earth's core, which has 6000 degrees Celsius of heat. At any moment, this heat transfers to the surface of the earth. Therefore, the energy inside the earth must be removed, and by transferring the water and salt solution that all the oil extraction companies have, which is known as the injection of water and salt solution, like a tiny needle that is inserted into a balloon so that the balloon does not burst, we humans can create an artificial earthquake. Let's prevent the earthquake explosion and create an artificial earthquake ourselves and release the pressure inside the earth. And 3, we should not build residential or office buildings where there is a fault line, because the buildings are heavy and the taller and bigger they are, the more pressure is placed on the faults. So either we have to build a single floor or not at all to prevent an earthquake from happening.
Wisam Fawzi added an answer
I saw that this technique is used in most Iranian structures and my personal opinion is a successful technique.
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Did you used technics of Ioannis Lymperis ?
Regards,
Laszlo
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How to make buildings resistant to earthquakes?
Now in Iran, according to my suggestion, Unilit roof is used in the roofs of residential and office buildings, which is very light. I took this suggestion in an article for the seismological organization in Tehran and gave 14 suggestions to prevent the Tehran earthquake, including 2 They implemented it. One of them removed the bricks from the roof of residential and office buildings and put unilite and poured concrete on top of it, which is very resistant because there is a round rod inside the bits and it was mixed with concrete, and I also said that in metal buildings from 7 or 8 should be used next to the walls because it makes the Masguni houses stronger and also 2 parking spaces should be used under the buildings, like palm trees or dates, which have deep roots and will not fall during an earthquake. Buildings must have deep roots and also in the science of retrofitting structures, divergence is used, that is, natural or artificial rubber is used under the pillars of the houses, and steel springs are used in the middle, so that during an earthquake, the building, like a car or A car that has a spring and the springs play, the building goes up and down but does not fall, and this is a building engineering science that makes buildings resistant to earthquakes and natural disasters. And secondly, through the injection of water and salt solution, the energy of the faults can be removed. Because it comes from the earth's core, which has 6000 degrees Celsius of heat. At any moment, this heat transfers to the surface of the earth. Therefore, the energy inside the earth must be removed, and by transferring the water and salt solution that all the oil extraction companies have, which is known as the injection of water and salt solution, like a tiny needle that is inserted into a balloon so that the balloon does not burst, we humans can create an artificial earthquake. Let's prevent the earthquake explosion and create an artificial earthquake ourselves and release the pressure inside the earth. And 3, we should not build residential or office buildings where there is a fault line, because the buildings are heavy and the taller and bigger they are, the more pressure is placed on the faults. So either we have to build a single floor or not at all to prevent an earthquake from happening.
How to make buildings resistant to earthquakes?
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I saw that this technique is used in most Iranian structures and my personal opinion is a successful technique.
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I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately after elution. After testing various pH levels and salt concentrations, I have been able to stabilize it in MES buffer at pH 5.5 with 1M salt and 5% glycerol, by immediately diluting it after collection. The highest concentration I achieved was approximately 6.67 mg/mL, which was only possible by adding 50 mM EDTA post-elution. This addition seemed to stabilize the protein, but I am uncertain if this approach is optimal, and replicating the results has been challenging.
However, when I try to concentrate it using a concentrator, its concentration rapidly decreases after buffer exchange. I have tested the flow-through, and the protein is not present there. I have also tried flushing the concentrator membrane with buffer, but there is no protein stuck to the membrane either. Only a negligible amount is precipitated. I am unable to determine what is happening to the protein. My eventual goal is to crystallize the protein.
Thank you.
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Have you tried including a substrate during and after the purification to help with stability? Keeping the protein concentration as low as practical should help reduce precipitation. For crystallization, it may not be necessary to have the concentration very high, since the protein seems to be prone to self-associate.
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I need the calculation procedure for this
Thank You
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  • Determine the formula weight of KNO3, which is approximately 101.1 g/mol.
  • Since the atomic weight of nitrogen (N) in KNO3 is approximately 14 g/mol, 100 mg of KNO3 contains 14 mg of N.
  • To prepare a 1000 ppm NO3 solution, you would need 1000 mg of NO3 per liter of solution.
  • Therefore, you would need to dissolve 1000 mg of KNO3 in 1 liter of water to obtain a 1000 ppm NO3 solution
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I am researching on azo dye degradation by bacteria. To analyze FTIR, how should I prepare my samples? Do I need to centrifuge, add anything or subtract anything? I am using minimal salt medium with 0.25% glucose.
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Depends on the FTIR method you are using, I guess you are using a configuration suitable for measuring liquids. First thing I would do is measure the media-bacteria-dye solution spectrum and media-bacteria solution spectrum. First check that it is correct spectra (absorption intensity, presence of relevant peaks...) and then see if there is any characteristic band corresponding to the dye. If there is, you can quantify dye degradation using that peak, if there is not... try processing the sample so you get a nicer spectrum, maybe.
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I am already using BL21(DE3)pLysS cells but I am still getting leaky expression in pre-expression samples before IPTG is added. LB broth is tryptone, salt and yeast.
Thanks :)
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Add to 0.2% glucose.
Glucose has to be filtered sterilized not autoclaved.
If this does not work, you may want to reduced the plasmid copy number by introducing the pncB mutation (described by Jon Beckwith).
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I need to a salt without any nitrogen in it
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Adam's Fusion, also known as the alkaline fusion method, is typically used for the determination of halogens, sulfur, and other elements in organic compounds. Sodium nitrate (NaNO₃) is commonly used as an oxidizing agent in this procedure. If you're looking for an alternative to NaNO₃, there are several other oxidizing agents you might consider, each with its own benefits and considerations.
Some Alternative here
1. Potassium Nitrate (KNO₃):
- Potassium nitrate is a strong oxidizer similar to sodium nitrate and can often be used interchangeably in chemical reactions.
- It provides good oxidation and can be readily available.
2. Sodium Peroxide (Na₂O₂):
- Sodium peroxide is a powerful oxidizing agent and can be used in fusion processes.
- It provides a strong oxidizing environment but must be handled with care due to its highly reactive nature.
3. Potassium Peroxide (K₂O₂):
- Similar to sodium peroxide, potassium peroxide can be used as a strong oxidizer in fusion methods.
- It also requires careful handling and storage due to its reactivity.
4. Sodium Persulfate (Na₂S₂O₈):
- Sodium persulfate is another strong oxidizer that can be used in place of sodium nitrate.
- It decomposes to produce oxygen and sulfate radicals, providing a strong oxidizing environment.
5. Potassium Persulfate (K₂S₂O₈):
- Like sodium persulfate, potassium persulfate is a strong oxidizing agent that can be used in fusion reactions.
- It is effective but requires proper storage to prevent decomposition.
6. Sodium Perborate (NaBO₃):
Sodium perborate can serve as an alternative oxidizer, particularly in less demanding oxidation reactions.
It is less aggressive than peroxides but still provides an oxidative environment.
Considerations When Choosing an Alternative:
Oxidizing Strength: Ensure that the alternative oxidizer is strong enough to achieve the desired oxidation in your specific fusion reaction.
Handling and Safety: Some oxidizers (like peroxides) are more reactive and require careful handling, storage, and disposal procedures.
Reaction Conditions: Consider the compatibility of the oxidizer with other reagents and the reaction conditions (temperature, solvent, etc.).
Availability and Cost: Choose an oxidizer that is readily available and cost-effective for your laboratory or industrial application.
When selecting an alternative to NaNO₃, it's crucial to consider the specific requirements of your Adam's Fusion process and the properties of the alternative oxidizer to ensure a successful and safe reaction.
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Hi everyone!
I currently have the issue that my proteins are all eluting at the salt wash that I apply during my IMAC (HisTrap 5ml HP column).
My proteins are all His-SUMO-tagged. They are followed by a highly charged domain that should have a structure (alpha-helical but with a small unstructured region as well) and is very positively charged. After that, there is a domain that binds the cell wall of bacteria and another domain that should be cleaving the cell wall of bacteria.
I have been working on this type of protein for the last two months, and the only thing that is different among all constructs is just the positively charged domain between the N-term His-SUMO-tag and the other two domains on the C-term.
Usually, I load the sample in 20mM HEPES pH 7.4, 300mM NaCl, 1mM DTT, 5% Glycerol, 20mM Imidazole on the column, perform a wash with the same buffer with 50mM Imidazole and then another wash with 1M NaCl containing 20mM Imidazole. My first constructs were totally fine with this procedure and did not elute in the salt wash (just in the actual elution with 300mM Imidazole).
Then, after I tested different constructs that differed in the charged domain, I started seeing that every construct I test, is eluting in the salt wash. The only thing that changed, besides that one domain, between those constructs is that I started expressing the latter ones in TB autoinduction (25°C, 1.5% Lactose) due to time reasons (just too much constructs to express and test). Before, where I saw no issues, I was expressing in LB at 16°C with 0.1mM IPTG.
Might this be one reason that these constructs now behave so strange? The expression in TB always looks perfect. A lot of protein. But might this be the issue? Is it too much protein? Are they aggregating already? Does this change anything concerning electrostatic interactions? Does anyone have experience with this issue?
I have to mention that we started implementing a high salt wash in every E. coli purification since we always have a high 260nm signal. So, my idea would be to simply perform a normal IMAC without a salt wash in the future. Then, to remove the DNA in the flow through, I could perform a cation exchange (constructs are all around pI 10). Or would you rather actively bind the DNA with an anion exchange and collect the protein of interest in the flow through? Then, I cleave the protein with SUMO protease and remove the tag via size exclusion.
Alternatively, I heard that PEI could be used to initially precipitate in the lysate DNA? I just know this reagent from using it as a transfection reagent.
Benzonase is in our lysis buffer anyway, but it doesn't seem to help the removal of nucleotides in our sample.
Again, I am happy for insights, suggestions and fruitful discussions.
All the best!
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If the wall binding domain has a positive charge and the Histag has a negative charge (at pH 7.4), they may bind to one another. Raising NaCl content (0.5M? 1M?) from the loading step may reduce electrostatic interactions and allow the Histag to bind the column.
You can also increase the imidazole gradient up to 0.5M, just in case the Histag gives you more trouble.
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Standard reagents (H2SO4, antimony, molybdate then ascorbic acid). Normal samples (water washed biochar, which would remove some salts plus a few nutrients, thus the PO4 test). Instant reaction turning the mixture white. I have done P analysis on thousands of aqueous samples of all kinds of sources and never seen this. Any suggestions are appreciated.
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Hi Paul, thanks very much for the thinking on this. The vexing thing is that we prepared 8 samples, all basically the same way (10g biochar with 20ml DI water, shaken overnight), the only difference being the source of biomass and the firing temp of the char. Only two samples had this milky white response to the reagent. I took the pH of all the samples, and the anomalous one was actually the lowest pH (range was 10 to 12), coming in at 10. It sure looks like a precipitate, but the confusing part is that none of the other samples behave like this, and I have done thousands of samples in my career and never seen this. I suppose I could acidify the sample, catch and dry the precipitate and run it on XRD.
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Hello!
I am doing batch adsorption studies for removal of lead. I am using lead nitrate salt for this purpose. When I adjust the initial pH of solution to 5 or above it precipitates. However, in literature researchers reported initial pH of solution to 9 as well performing batch adsorption studies. Is because of salt or something else?
Thanks for your guidance.
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Depends on a reagent that you use for adjusting pH.
Phreeqc modeling shows that pH of 1 m Pb(NO3)2 solution is close to 5.4.
Adjusting pH to higher values requires addition of base and can cause precipitation of Pb(OH)2.
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Hi.
I'm working on annealing of Al5083 and need to anneal that in salt bath. The temperature range of my experiments are between 200-500. I'd be appreciate to receive the name of proper salt for this alloy.
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For annealing the aluminum alloy 5083, the proper salt to use is typically sodium chloride (NaCl), or common table salt.
Aluminum alloy 5083 is a non-heat-treatable alloy, meaning its mechanical properties cannot be enhanced through precipitation heat treatment like some other aluminum alloys. However, it can benefit from annealing, which is a heat treatment process that softens and increases the ductility of the alloy.
The annealing process for 5083 involves heating the alloy to a temperature around 650-775°F (343-413°C) and holding it at that temperature for a period of time, followed by controlled cooling. This heat treatment helps to relieve internal stresses, refine the grain structure, and improve formability.
To prevent surface oxidation during the high-temperature annealing process, the 5083 alloy is often immersed in a molten salt bath. Sodium chloride (NaCl) is the most commonly used salt for this purpose due to its low cost, availability, and suitable melting point.
The molten salt bath provides a protective atmosphere around the aluminum alloy, preventing atmospheric oxidation and allowing the annealing process to occur effectively. After annealing, the salt is removed from the surface of the alloy through a cleaning process.
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We are trying to find the best solvent concentration and storage conditions.
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Respected Mariana Colaço
To aliquot polymyxin B sulfate salt (P4932-1 MU) from Sigma-Aldrich, begin by preparing a stock solution of the desired concentration, following the instructions provided by Sigma-Aldrich or referencing the product datasheet. Once the stock solution is prepared, ensure proper sterilization techniques are employed to maintain purity and prevent contamination. Using an aseptic technique, dispense the desired volume of the stock solution into sterile aliquot tubes, taking care to avoid introducing air bubbles. Seal the aliquot tubes securely to prevent evaporation or contamination during storage. Label each aliquot tube with the date of preparation, concentration, and other relevant information. Store the aliquots at the recommended temperature and conditions specified by Sigma-Aldrich to maintain stability and functionality.
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protecting Secondary amine with acid halide in presence of NEt3 especially in Flow sytem?
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You can use a more polar solvent e.c., DMF where TEA hydrochloride dissolves fairly well. Alternatively, you can use Hunig's base (N-ethyl-N,N-diisopropylamine) in place of triethylamine. Hydrochlorides of Hunig's base are more readily soluble in organic solvents.
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Should I centrifuge the the crude microalgal sample first and then analyze it or Should I dry it first. Please Help me out to solve this.
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You should centrifigate the samples then dry them to prepare for TS
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Hello, I am currently conducting my Bachelor on a organic synthesis. Here, I first form an acid chloride by the addition of thionyl chloride and Dimethylformamide (which together form the Vilsmeier-Haack reagent) to my starting product (4-hydroxyphenylacetic acid) at room temperature. After, I add Triethylamine (a proton scavenger) and a dimethylamine HCl salt, the latter of which should react with the acid chloride in a amidation reaction. However, upon addition of the DMA.HCl nothing seems to occur and a IR-spec analysis of the final product shows a molecule reminiscent of polyethylene terephthalate (PET). This finding makes me think that there might be a polymerization reaction going on in which the acid chloride reacts with the hydroxy group of 4-hydroxyphenylacetic acid. This would also be able to explain as to why the DMA.HCl would not react in the mixture as all the acid chlorides would have polymerized before the amidation could occur.
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Dear Tom,
Maybe you should protect the hydroxyl group before amidation? Otherwise, the starting material 4-hydroxyphenylacetic acid will undergo esterification with itself.
Or you can use EDCI/DMAP/Et3N to amidate them instead?
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pls suggest process to extract nicotine from tobacco leaf to make nicotine salt
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Вакуумно-паровая экстракция из сырья завода и оборудование «Альфа-Эфир-Вакуум» для получения вакуумных экстрактов.
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Currently, I'm preparation a homopolymer sodium salt using Sodium Persulfate as initiator and sodium hydpophospoite as CTA. But,my polymer that I've got have high molecular weight. I need your advise how to decrease my polymer molecular weight besides add more volume of water in the initial of the reaction (before polymerization) because I know homopolymer is a solution polymerization so I think they need more solvent so that the molecular weight is lower than before
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Dear all, there are many possibilities to get the required MW. If you have the kinetics curve of the polymerization reaction, i. e., MW vs time or MW vs %conversion, then all to do is to quench the reaction at the time or % conversion corresponding to the targeted MW. The second solution is to do dialysis fractionation with a memebrane with the specific MW cut-off. The third possibility is to do mechanical degradation by shearing either by high speed agitator or passing through low porosity sieves.
If you want to avoid these extra work, choose a solvent with high transfer constant, and reduce the polymerization temperature. Starve feeding of the monomer may also help to reach a moderate MW. My Regards
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When mixing the Algal Trace Elements Solution for COMBO medium, I had the problem that there was insoluable precipitation before and after autoclaving (in several trials). (Re)heating and stirring could not do anything.
I am afraid the same will happen again as there are particles floating around after adding metal solutions before autoclaving. But I really do not know what I did wrong. I dissolved NaEDTA well in MiliQ water before adding FeCl3 to it, dissolving it als well and I carefully put in the metal salt solutions in given order, everything like I did before. Before I try it next again, I might need to change something... What do you think? Should I decrease the concentration of metals by a certain factor (maybe 10) and put in more of that in the final medium for the right end concentration?
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I am having a similar problem to this now. Did you ever figure out why? Right not we are trying stirring.
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Hi.....I am working on a science project with my grandkids identifying crystals and have hit a real puzzler. We took some brine from a store-bought sour cabbage to see what might crystallize from the brine as it evaporated. In making the sour cabbage the producer said they added only 2% by weight Kosher salt and let the cabbage ferment for 2 weeks. The cabbage was not pasteurized.
We expected to see cubic NaCl crystals but, along with the normal salt crystals, was odd looking crystalline material as shown in the attached images taken with a binocular mic. The crystal morphology varies from snow-like to serrated laths to a cross-hatch fabric.
My guess is the material might be a product of fermentation. Any help would be greatly appreciated.
thank you
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Congratulations on your idea, creativity, and practice exercises with the younger generation!! I believe in previously advises. Consider doing X-ray diffraction measurement to confirm it.
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Salt water does not evaporate faster than fresh water; in fact, fresh water always evaporates faster than salt water. This is because of the difference between the salt and water molecules. Water is a slightly volatile substance, meaning that it is capable of evaporation, while salt is a nonvolatile substance, so it is not as capable of evaporation.
Evaporation takes place on the surface of a substance. Fresh water has only water molecules on its surface area, which lets it evaporate easily. Salt water, on the other hand, has both salt and water molecules on its surface area. The salt molecules take up part of the surface area and prevent the water molecules from evaporating as quickly, which is why salt water always evaporates more slowly than fresh water.
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Salt water does not evaporate faster than fresh water; in fact, fresh water always evaporates faster than salt water. This is because of the difference between the salt and water molecules. Water is a slightly volatile substance, meaning that it is capable of evaporation, while salt is a nonvolatile substance, so it is not as capable of evaporation.
Evaporation takes place on the surface of a substance. Fresh water has only water molecules on its surface area, which lets it evaporate easily. Salt water, on the other hand, has both salt and water molecules on its surface area. The salt molecules take up part of the surface area and prevent the water molecules from evaporating as quickly, which is why salt water always evaporates more slowly than fresh water.
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I’m currently using a protein (36 kDa) which needs to be unfolded during a labelling reaction. unfortunately the protein precipitates completely upon denaturation with EDTA which chelates the zinc ions holding its structure together. I’ve tried the reaction at 37, 40, and 55 degrees Celsius all with the same issue.
The exact same protocol (37 degrees, same edta:zinc ratio, time course) works for smaller constructs of the protein. I typically unfold, reduce, and then add the labelling reagent sequentially for 50 minutes each (total 2.5 hours shaking at 37). My protein concentrations have been between 20 and 200 micromolar, and the pH is maintained at 7.8 in 100 mM ammonium bicarbonate buffer (no salt) for optimal labelling.
I need the protein to remain in solution for downstream experiments after the labelling reaction. How can I denature the protein while keeping it soluble?
The protein pI is 7.06, which may be too close to the reaction buffer, especially considering that the other constructs had pi’s at 5.3 and 6.6. I’m considering testing a higher pH, unfolding with EDTA and a detergent, and increasing the salt concentration. Ideally I’d have as low a salt concentration as possible for downstream mass spec, and maintain the pH between 7.5 and 8.5 for optimal labelling with the different reagents. I’d appreciate any feedback or advice!
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There are 2 main reasons why the protein could be precipitating: electrostatic interactions or hydrophobic interactions.
Increasing the salt concentration would probably overcome electrostatic interactions. You would have to remove the salt later, which is easy to do by passing the column through a desalting column or by dialysis. Changing the pH by several units could also help, but you have explained why this is a less desirable approach for you.
In my opinion, the more likely reason for the insolubility is hydrophobic interactions, since the interior parts of proteins are hydrophobic. You could probably keep the protein in solution using a detergent, but removing detergent later can be troublesome. There are a few detergents that are compatible with mass spec, however. The other approach would be to add a strong chaotrope, either urea or guanidine-HCl, at several molar. These are small molecules that can be removed later. You would have to experiment to find the minimum necessary concentration to keep the Zn2+-free protein in solution, especially if you don't want to completely unfold the protein, since refolding a completely denatured protein can be a challenge.
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Please suggest me any paper for this. Is there any process to collect them and find out in lab?
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To estimate carbon stocks in salt marshes, seagrasses, or mangroves, you typically conduct field measurements and use established methods such as:Vegetation Surveys: Measure the biomass and density of plant species in the area.Soil Sampling: Collect soil cores to measure carbon content at different depths.Remote Sensing: Use satellite imagery or aerial surveys to estimate vegetation cover and biomass.Allometric Equations: Utilize equations that relate plant size (e.g., diameter, height) to biomass.Carbon Content Analysis: Analyze collected plant and soil samples in the laboratory to determine carbon content.GIS Mapping: Combine field data with geographic information system (GIS) tools to create spatial maps of carbon stocks.
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Is there a direct and simple relationship between the solubility of a salt and the dielectric constant of the solvent or solvent mixture in which the salt is to be solubilised?
For example: the saturation molality of NaCl in pure water at 25°C is about 6.14 molal. The saturation molality of NaCl at 25°C, either in mixtures of water and formamide (the dielectric constant of formamide is much higher than that of water) or in mixtures of water and ethanol (the dielectric constant of ethanol is much lower than that of water) decreases in both cases.
I have not found any co-solvent that increases the solubility of NaCl compared to that of pure water. The same is true for all alkali halides!
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The dielectric constant tells us how well a solvent can decrease the force between charged particles. Basically, a high dielectric constant means the solvent is really good at pulling ions apart, which usually makes it easier for salts to dissolve in it. Water is a prime example—it has a high dielectric constant, making it excellent for dissolving many salts.
But, the story doesn't end there. Several other factors play into how well a salt dissolves:
  • The specific interactions between the solvent molecules and the ions (ion-dipole interactions) matter a lot. Even if a solvent has a high dielectric constant, its molecular structure and how it can form bonds or interactions (like hydrogen bonds) significantly affect its ability to dissolve salts.
  • When you mix solvents (like water with formamide or ethanol), things get tricky. The mix affects solubility not just based on the combined dielectric constant, but also on how these solvents interact with each other and with the salt.
  • In these mixes, how ions pair up or clump together can change, which also influences how much salt can dissolve. The mix can shift the balance between ions sticking together and being free in the solution, changing solubility.
For NaCl and similar salts, water's super high dielectric constant plus its knack for forming strong hydrogen bonds make it especially good at dissolving salts. Adding another solvent into the mix, whether it's formamide (which has a different way of bonding despite its high dielectric constant) or ethanol (which has a lower dielectric constant and isn't as good at stabilizing ions), tends to mess up the delicate balance that makes water so effective on its own, usually making the salt less soluble.
The fact that no added solvent makes NaCl more soluble than it is in pure water highlights how uniquely suited water is for dissolving ionic compounds like salts.
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Provided that the cultured cells were supplemented with sufficient metal salts during their growth phase
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I'm agree with Adam B Shapiro
since in general the affinity of metalloproteins is in micro molar range is it possible that at the end of purification process, you will obtain an apo or partially metallated form, expecially if you are using for purification approaches as IMAC where there are also different metals that can interfeere.
I suggest to you to produce apo form by incubating the protein with EDTA, remove the EDTA by desalting and add again the metal.
you can use DSF and check how the tm change under metal addition to check if the protein bind the metal..
in the following paper you can find an example of this on the following paper
good luck
Manuele
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Hello,
I am currently working on a cyclic voltammetry test for ferrocene in acetonitrile I would like to ask you about some details, please:
-which work and counter electrode do you use?
-What cell do you use for measurement?
-How is the measurement process done?
-What potential range do you use?
-wich scanrat (v/s) ?
-How many repetitions are required?
Sorry for the large amount of questions, but the results I get are not completely satisfactory،I use a platinum wire as a reference electrode, platinum electrode as work electrode and glass carbon as counter electrode in acetonitrile,1mM Ferrocen and 100 mM TBAP as conductive salt.
An ordinary beaker from the laboratory was used as a cell.
I greatly appreciate your response and I would be grateful if I could get answers from you.
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I am using Ag/AgCl electrode for alkaline seawater splitting. As 1M KOH is not suitable for Ag/AgCl nbecause high alkaline condition electrode potential is Ag2O. I am using 0.5M KOH. For extra care we rinsed the electrode with 3M KCl solution before each measurement. We have Hg/HgO electrode but I found that Hg is sensitive to chloride anion. We know that salt concentration is high for sea water condition. Will it be accurate or minimum error in electrode potential in this condition (seawater+0.5M KOH)?
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This is a fascinating application. Let's delve into the option:
1. Ag/AgCl Electrode:
○ The Ag/AgCl electrode is widely used as a reference electrode in electrochemical measurements.
○ It provides a stable and reproducible reference potential.
○ The Ag/AgCl system maintains a constant potential due to the reversible Cl^-/AgCl redox couple.
2. Seawater Splitting:
○ Seawater splitting for hydrogen evolution is a promising clean hydrogen energy harvesting technique.
○ The goal is to generate hydrogen gas (H₂) from seawater through electrolysis.
3. Electrolyte Choice:
○ You mentioned using 0.5M KOH instead of 1M KOH.
○ This is a good choice because high alkaline conditions can lead to the formation of Ag₂O on the Ag/AgCl electrode, affecting its potential.
○ Lowering the KOH concentration helps mitigate this issue.
4. Rinsing with 3M KCl Solution:
○ Rinsing the electrode with 3M KCl solution before each measurement is prudent.
○ KCl helps maintain the stability of the Ag/AgCl electrode by replenishing the Cl^- ions.
5. Hg/HgO Electrode Sensitivity:
○ You mentioned having a Hg/HgO electrode.
○ Hg is indeed sensitive to chloride anions.
○ In seawater conditions, where chloride concentration is high, this sensitivity could introduce errors in electrode potential measurements.
6. Accuracy and Error:
○ The accuracy of electrode potential measurements depends on several factors:
Ionic strength: Seawater has a high ionic strength due to dissolved salts. This affects the double-layer capacitance and can impact the electrode potential.
Chloride concentration: The presence of chloride ions affects the Ag/AgCl electrode potential.
KOH concentration: Lowering the KOH concentration helps minimize Ag₂O formation.
○ While the Ag/AgCl electrode is commonly used in seawater studies, it's essential to consider these factors and calibrate accordingly.
Using the Ag/AgCl electrode in seawater with 0.5M KOH and proper rinsing procedures should yield reasonably accurate results. However, be mindful of the Hg/HgO electrode's sensitivity to chloride ions. Regular calibration and validation against known standards are crucial for minimizing errors in electrode potential measurements.
Source(s)
1. Seawater splitting for hydrogen evolution by robust electrocatalysts ...
2. Investigations into electrochemical water splitting - Enlighten Theses
3. Impedance characteristics for solid Ag/AgCl electrode used ... - Springer
4. Insights on the Electrocatalytic Seawater Splitting at Heterogeneous ...
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I'm trying to measure a few (typically in the range of 0.5mg to 5mg) micrograms of organic compounds for a particular experiment on a microanalytical balance (A&D BM20).
The apparatus I'm currently using:-
  1. Micro spatula (steel) - I mostly use the curved side as a quarter scoop that usually results in 0.5mg of compound.
  2. Butter Paper - thickness is less than 0.1mm.
  3. Microanalytical balance (A&D BM20) - I use the ion function to remove any loss / sticking due to static charge.
The problems that I'm facing are as follows:-
  1. The organic compound is hygroscopic, and hence, a little exposure to air results in the salt sticking to the micros spatula.
  2. Only a few grains of the compounds are enough for the required weight, but most of the time these are lost while transferring it to the solution vial, mostly stuck on the butter paper.
Hence I'm looking for alternatives for butter paper or any other methods to improve my measuring accuracy.
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Have you tried aluminum foil? There are also weighing funnel shaped like a "spoon with a funnel", so what is being weighed can be rinsed out. Another option is plastic weighing boats, small bendable bowls.
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One day when we made our regularly used medium, we noticed the initial pH was much higher than usual (above 6.0. usually it is around 4.0). It seems it only happened in our lab. The neighbor lab doesn't have problems. I have tried to test the initial pH using the water from our lab (B16) and the neighbor lab (B12). When I made LB medium, the initial pH was comparable with water from both labs; however, when making other media containing salts, vitamins, hormones, and sucrose, they showed different readings. The pH using water from neighbor lab was similar as our previous records while that using our lab was abnormally higher. I also used pH strips. The pH for water from the neighbor lab has been consistent, but that from our lab varied. Occasionally, it was similar; but most time shown in the attached file (shown in the attached file). Now my concern is what caused the high pH of this water. Is it toxic for our tissue culture? Any suggestions for figuring out the problem? Any information will be appreciated.
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Hi James, thank you again for this valuable information. The weird thing is that we share the same source of DI water (cartidge/tank) with other labs. Our neighbor lab (B12) has the DI water with consistent pH while ours (B16) started to suddenly have the abnormal pH since that day we noticed. The pH strips shown here are for the water from both labs (no any additives). I bought a conductivity meter and I will check the water quality when we have it. I am wondering if there is a leak somewhere in the pipe leading to our lab only.
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What material would you recommend that has interconnected holes and is abundant in nature, making its extraction or production cost low and the production process simple? This material should have the ability to retain a significant amount of salt (NaCl) and gradually release it when in contact with ice. Additionally, it should be a one-time use material.
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Expanded Clay Aggregate (ECA) is an ideal material possessing the qualities of porosity, abundance, cost-effectiveness, simplicity in production, and salt-holding capabilities. As a lightweight ceramic substance, ECA is formed by expanding clay at elevated temperatures. Its porous structure renders it versatile for applications like water filtration, soil improvement, and as a growth medium for plants in hydroponic systems.
Key attributes of ECA that align with your needs include:
  1. Porosity: The porous structure of expanded clay aggregate, featuring open pores capable of holding and releasing water, proves advantageous in water filtration systems and facilitates effective drainage in plant cultivation.
  2. Abundance: Derived from naturally abundant clay resources, expanded clay aggregate stands out as a readily available material, often sourced from natural clay deposits.
  3. Low-cost: The production process for expanded clay aggregate is straightforward and cost-effective, contributing to its affordability in comparison to alternative porous materials.
  4. Simple to produce: The uncomplicated manufacturing process involves heating clay pellets in a rotary kiln, inducing expansion. This simplicity eliminates the need for intricate production techniques.
  5. Capable of holding salt: Expanded clay aggregate exhibits the ability to retain salts without compromising its structure. This characteristic is particularly valuable in applications where salt retention is essential, such as certain types of water treatment.
While expanded clay aggregate meets these specified criteria, it is crucial to consider the specific requirements of your intended application. Evaluate whether alternative materials may better suit your needs, and also factor in considerations of environmental sustainability and the ecological impact of the chosen materials.
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short answer.
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Salt structures can be both beneficial and problematic for oil exploration and production. Their impact depends on various factors, including the specific geology of the area, the type of salt structure, and the presence of other essential elements for a hydrocarbon system.
It's important to note that modern exploration techniques, such as advanced seismic imaging and drilling technologies, are continuously improving the ability to overcome challenges associated with salt structures, making them valuable exploration targets despite the complexities involved.
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Hello
I prepared silver nanoparticles by various method but every time peak disappeared, as the peak of silver nitrate salt at 300nm and it disappeared when I reduced it chemically. Now suggest me whether I put these peak as it is or I find out other method for silver nanoparticles synthesis because in literature their peak appear at 350 to 400nm .
Kindly suggest the best way.
AToI
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Probably you run blank with suspention of AgNPs . Othervise it may be that you used unsiutable stabilising agent and AgNPs got agregated and therefore you dont have SPR peak. The second reason took place in my recent research too/
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Please provide atleast 4-5 methods to remove water from H3PO2 to make it concentrated except from using ether.
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we can remove water from 50% H3PO2 by:
- Simple distillation: Heat the solution to its boiling point, allowing water to evaporate and separate from H3PO2.
- Fractional distillation: Similar to simple distillation but more effective for separating liquids with closer boiling points.
- Vacuum distillation: Reduce the pressure to lower the boiling point of water, facilitating its separation from H3PO2 at a lower temperature.
- Drying agents: Add a desiccant like calcium chloride or silica gel to absorb water from the solution.
- Evaporation: Allow the solution to evaporate slowly under reduced pressure or elevated temperature to remove water gradually.
in my knowledge, as for a suitable base that reacts with H3PO2 to produce a salt without acting as a nucleophile, sodium hydroxide (NaOH) is a commonly used option. When NaOH reacts with H3PO2, it forms sodium phosphate (Na3PO3) and water, without involving nucleophilic reactions.
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Hello, I am seeing inconsistent passivation results in my stainless steel finishing process. I am welding 316 stainless steel, then grinding down the weld to achieve a smooth finish, then electro-cleaning the mechanically altered surface with a handheld electropolisher.
The problem is that sometimes I will get grooves in the ground surface during that step (chatter-like marks perpendicular to the grinding direction) that eventually rust in a salt spray test, even after electrochemical cleaning. I've attached a picture of the marks that show up after the grinding step.
Any ideas on how to avoid rust in this scenario? Is the grinding step thermally degrading the passivation layer of the stainless steel in a way that it cannot be salvaged?
Any feedback is appreciated! Thanks!
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Dear Dr. Amy Geary ,
could you attach the picture of the marks that show up after the grinding step? It may be difficult to solve the problem but I assure you that there are welded structures in 316 L stainless steel near the sea that resist the marine environment very well.
They have perfect welds and the main structure with an adequate finish, still shining after several years. Given the size, I don't think they receive particular care... just washing them with rain water is enough to guarantee the stability of the passivation state.
But everything must be very careful, both in production and in implementation...
My best regards, Pierluigi Traverso.
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Hello,
I actually have a salt of potassium formate, but the issue is that there are many impurities in the salt. I wanted to know which analytical technique is appropriate to quantify the composition of the salt as well as identify all the impurities present in the salt.
I am thinking of HPLC and GC-MS. Which of these is better and how to go about it? Lastly, are there any better methods than the ones I have listed? There is a lab with most of the equipment, but I just want to make sure I go with the most suitable one.
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Dear Alfred,
Thanks, I will try out different methods surely and update if I get any conclusive results.
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Imagine you have a field with excessive salt buildup. Assume the volumetric water content is 0.35 cm3 cm-3 and the root zone is 120 cm deep.
If the soil in the field is a clay loam soil with Ks= 5.5 cm per day, b = 5.2, and saturated water content (Theta)=0.5 cm3 cm-3, the hydraulic conductivity followed Campbell’s model, and the flow was under unit gradient conditions (i.e. negligible matric potential gradient), then how long would it take to leach out the salts?
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The leaching process duration can be estimated using the leaching requirement formula:
t = H/K
where:
- T is the leaching time,
- H is the leaching requirement (difference between initial and desired salt content), and
- K is the hydraulic conductivity.
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Hello, I was testing the corrosion rate of Aluminium when dissolved in a highly concentrated potassium formate. Now I know corrosion rate is influenced by the presence of dissolved oxygen as well as the temperature. I actually wanted to get a standpoint on how the corrosion rate would be affected at a constant concentration of the salt solution and also the impact on dissolved oxygen.
Is there someone who can guide me on this?
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Pavan Kumar Dear colleague! Sodium formate is a salt of a weak acid and a strong alkali and gives an alkaline reaction in water. Therefore, it causes corrosion of some metals and alloys. Formate is a salt, which means it promotes electrochemical corrosion. Formate can also be a ligand, which means it accelerates the corrosion of non-ferrous metals.
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After the preparation of Graphene Oxide (GO), from modified Hummers method there are two recommended washing steps: (i) with HCl and (ii) then with de-ionised water. Is it advisable to make the PH to neutral by addition of base like NaOH instead of washing with de-ionised water several times to remove acid  >??? 
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Dear V S,
Mind if I ask how you decided to proceed, and what was the outcome in the end?
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The deal is about HyJet (Exxon), and analogs (Valvoline Ultramax and Solutia/Eastman Skydrol).
Aviation hydraulic fluids based on fire resistant alkyl and/or aryl phenyl phosphate esters may contain PFAS additives such as cyclohexanesulfonic acid, decafluoro(pentafluoroethyl), potassium salt (CAS No. 67584-42-3) and different chain-length homologs in concentrations of about 0.05% . Other possible substances are cyclohexanesulfonic acid, decafluoro(trifluoromethyl)-, Potassium salt, CAS No 68156-07-0, cylohexanesulfonic acid, nonafluorobis(trifluoromethyl)-, Potassium salt, CAS No. 68156-01-4 or cyclohexanesulfonic acid, undecafluoro-, Potassium salt, CAS No.3107-18-4
These additives are extremely effective and thermally and electrochemically stable, but PFAS are now banned.
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I have no specific and direct experience in this sector. But technically reasoning may suggest that since PFAS are banned the problem should be solved (if not already solved and present in tech-literature) experimentally, by specific research.
As already well known:
1. Zeta potential is shown by any particle in suspension, macromolecule or material surface.
2. Zeta potential can be measured by electrophoresis .
3. Electrophoresis measures can be used to optimize the formulations of suspensions, emulsions and predict interactions with surfaces, and optimise the formation of films and coatings.
4. As the salt content of the solution is increased, the electrical double layer is compressed and the Zeta potential should decrease.
5. Different kind salt can be experimented in different solutions in a lab configuration under mesurament, keeping also in mind the possibility to decrease the negative potential of the solution by means of counterbalancing with a minimum positive potential indotto (where possible!).
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I am doing a fluorescence spectroscopy experiment of a nafion thin film with HPTS dye and running it using salt solutions for humidity. But, at High Humidity such as 70% it starts condensation inside the chamber ( a closed box ; inside it I keep my sample to humidify).
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No, i did not get such error thing.
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I have performed the Electrochemical CO2 Reduction in 0.1M NaHCO3.
I have to detect the liquid products from the HPLC instrument. What kind of column can be preferable for detection? I have C18 column only.
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Did you perform HPLC?
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why not any one?
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Hey there Adarsh Shetty! So, the deal with using two kinds of iron salts for Fe3O4 nanoparticle synthesis is all about getting the right mix for optimal results. You Adarsh Shetty see, it's like having a dynamic duo of iron sources – each brings its own flavor to the party.
First off, we've got our ferrous salt, the humble Fe2+. It's like the laid-back, easygoing sidekick. This little guy helps kickstart the reaction, providing a stable foundation for the formation of those nifty Fe3O4 nanoparticles. Think of it as the calm before the storm.
Then comes our ferric salt, the feisty Fe3+. This one's the firecracker, injecting some energy into the mix. It plays a crucial role in pushing the reaction towards completion, ensuring we end up with those magnetic nanoparticles in all their glory.
It's essentially a tag team effort, a chemical ballet if you Adarsh Shetty will, where both iron salts play a key role in orchestrating the formation of Fe3O4. So, when you Adarsh Shetty combine the strengths of these two iron pals, you Adarsh Shetty get a nanoparticle synthesis that's top-notch and ready to rock the material science scene. Cool, huh Adarsh Shetty?
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Hello, i prepared porous carbon using direct and salt template method. i thought the salt template should only give mesoporous carbon but i got hysteresis in the two methods. How do i explain that the salt template improved the porosity more than the direct carbonization
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Dear Chinedu Onyeke According to your results, the molten salt approach produces more porosity. The explanation for this is the mechanism of pore development in the molten salt process. Molten salt functions as a confined reaction chamber, creating an environment where salt ions infiltrate into carbon structure during high-temperature treatment and create well-defined pores. Nature of these pores, whether micro, meso, or macro, is associated with several critical parameters, including the size of the salt ions, the annealing temperature, and the duration of the process. Relevant literature can provide deeper understanding.
Best
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I am working on pyridylamine compounds as nucleophiles for a reaction. During the work, I encountered an amine which is in the form of a Hydrochloride salt. To perform my reaction, I need to completely remove it from the salt state and use its free amine. I can't extract it with the help of dichloromethane and water because the solubility of the this amine in free form is 20 times its salt state in water.
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There are two approaches for such reactions:
1) You can use Na2CO3 or NaHCO3 or any weak base to neutralize the hydrochloride salt while simultaneously monitoring the pH using pH paper.
OR
2) You can use KH2PO4 as a buffer to neutralize the effect of HCl and enable the availability of your amine for nucleophilic reaction.
The second method is more productive from my experience. However, every organic reaction is unique and doesn't behave ideally for all cases and exceptions do occur. Only after trying can one ascertain with conviction that the method is successful.
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During RNA FISH, the fluorescent probes are hybridized with target RNAs with 20-24bp base pairing.
But how strong this interaction is with only several hydogen bonds?
How much salt or fomamide are needed for break this interaction?
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You can check NUPACK to calculate your exact break solution concentration. Moreover you can just use DNaseI to remove the probes or use high concentration formamide(about ~60%)
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Dear all,
I want to express my protein of intterest in absence of salt. Now the challenge is to see the survival of cells and even if it survives, where I could find a cell medium (any company that provide it). Please recommend if any commerically available media that has no salt.
Thank you
With kind regards
Prem
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Hi Prem
There are several salt-free media for mammalian cell culture, including DMEM w/o NaCl, IMDM w/o Na/Cl, etc. However, salt-free media refer to the one without NaCl, they may still contain other salts such as KCl. These salts are often necessary for cell growth and function. Thus, I'm afraid there may be no completely salt-free media.
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i want to model a flow through a channel in which water flows from left end and on the top wall a salt concentration is to be provided. i want to see how much salt disolved in water in 100 sec. The water flows in the channel with a certain velocity. i do not understand how. i can apply salt composition on wall
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Yes concentration gradient can be created, fluid flow can be govern using this software