Saccharomyces - Science topic

Mexican wild grapevine plants (Vitis spp.) are important for wine and table grape industries, because their phylloxera resistance contribution to rootstocks breeding programs. Nevertheless, in Central Mexico, native Vitis are in risk of disappear because the preference to produce high value horticultural commodities. Participation in germplasm cons...

Bioresource collections represent a unique source of biological diversity for research in genetics and related disciplines. The Department of Genetics and Biotechnology of St. Petersburg State University is the oldest department of genetics in Russia, founded in 1919. Throughout the entire period of development, the geneticists of St. Petersburg Un...

Beer is a world-famous beverage, second only to tea and coffee, where the yeasts traditionally used are Saccharomyces cerevisiae and Saccharomyces pastorianus for the production of ale and lager beer, respectively. Their production, especially craft beer production, has grown in recent years, as has the development of new products. For this reason,...

Mezcal is an alcoholic beverage obtained by distilling musts and juices fermented by spontaneous or cultivated microorganisms, which are extracted from ripe stems of cooked Agaves and harvested in Mexico. Both raw material and production practices differ markedly between producing regions, locations, and even factories, resulting in a very distinct...

Aquatic animals are continually being exposed to stressors under farming conditions, increasing risks to gut health that result in dysbiosis. Since restrictions are imposed on the frequent use of antibiotics in aquaculture, there is emerging demand for economically viable, environmentally safe, and sustainable alternatives for the intensive product...

Introduction Eukaryotic life depends on the functional elements encoded by both the nuclear genome and organellar genomes, such as those contained within the mitochondria. The content, size, and structure of the mitochondrial genome varies across organisms with potentially large implications for phenotypic variance and resulting evolutionary trajec...

Sherry wines undergo a complex, two-stage production process. Initially, the Palomino Fino grape must undergo alcoholic fermentation, resulting in the base wine. This wine is fortified and enters the dynamic biological aging system known as “criaderas y soleras.” Despite the wide variety of wine yeasts available, there’s growing interest in develop...

The loss of aromatic and sensory quality in wines because of climate change in traditional winemaking areas is a challenge for winemakers. Aging on lees of the wine fermented with Saccharomyces native yeasts has been tested as a technique to try to improve the sensory characteristics of Malvasia aromatica white wines in PDO “Vinos de Madrid.” The g...

Palm wine is a traditional alcoholic drink and acts as an excellent substrate for microbial growth. It was obtained from the sap of various types of palm trees. Palm wine has several nutritional, medical, and social uses, which have been reported to have increasingly enhanced the demand for this natural product. However, the sap undergoes spontaneo...

Microorganisms play an important role in regulating flavor compounds in rice wine, whereas we often don’t understand how did they affect flavor compounds. Here, the relations between flavor compounds and microbial community ecological succession were investigated by monitoring flavor compounds and microbial community throughout the fermentation sta...

Background The aim of this systematic review is to evaluate the changes in gut microbiota (GM) induced by the Ketogenic Diets (KD) as a potential underlying mechanism in the improvement of neurological diseases. Methods A comprehensive search was conducted on three electronic databases, including PubMed/Medline, Web of Science, and Scopus until De...

In the present investigations efforts were been made to standardize the process for the production of pomegranate based wine. Bhagwa variety of pomegranate was selected for the production of wine. Bhagwa Variety of pomegranate were assessed for its nutritional composition and selected for the production of wine. Yeast Saccharomyces Cervisiae was im...

Dry yeast Saccharomyces. cerevisiae was obtained from markets in Kirkuk and used to study their ability to prepare silver nanoparticles and shows that the silver nitrate solution at 1Mm changes its color to brown and is an initial indication of the formation of nanoparticle particles and X-ray diffraction peaks were measured at (111), (202) and (22...

The discharge of chromium (Cr)-containing wastewater into the environment causes carcinogenic, teratogenic, and lethal effects on humans. Cr(VI) biosorption using free extracellular polymeric substances (EPSs) became an emerging eco-friendly technique. But it has the disadvantages of easy washout, slow adsorption capacity, and reusability difficult...

The Saccharomyces species have diverged in their thermal growth profile. Both Saccharomyces cerevisiae and Saccharomyces paradoxus grow at temperatures well above the maximum growth temperature of Saccharomyces kudriavzevii and Saccharomyces uvarum but grow more poorly at lower temperatures. In response to thermal shifts, organisms activate a stres...

Two strains of Saccharomyces cerevisiae (Sc01 and Sc02) and one strain of Wickerhamomyces anomalus (Wa) were isolated from organic Verdejo spontaneous fermentations and used for the development of experimental winemaking. Sc01 and Sc02 represented 52.7% of the population of the Saccharomyces strains isolated throughout the fermentation process. W....

Invasive fungal diseases are a public health problem. They affect a constantly increasing number of at-risk patients, and their incidence has risen in recent years. These opportunistic infections are mainly due to Candida sp. but less common or rare yeast infections should not be underestimated. These so-called “less common” yeasts include Ascomyco...

Ploidy is an evolutionarily labile trait, and its variation across the tree of life has profound impacts on evolutionary trajectories and life histories. The immediate consequences and molecular causes of ploidy variation on organismal fitness are frequently less clear, although extreme mating type skews in some fungi hint at links between cell typ...

The authors would like to state that they inadvertently omitted one of the authors when preparing the manuscript. The authors of this work are as follows: Miha Ocvirk1, Nataša Kočar Mlinarič2, Peter Raspor3 and Iztok Jože Košir1* 1Slovenian Institute of Hop Research and Brewing, C. Žalskega tabora 2, 3310 Žalec, Slovenia 2Pivovarna Laško Union d.o....

The aim of the study was to test the acceptability and physico-chemical characteristics of marula wine fermented with known cultures of natural Lactiplantibacillus plantarum and Saccharomyces cerevisiae. The LAB Lactobacillus and Saccharomyces dominate the fermentation of marula wine throughout the fermentation period. These were isolated and ident...

In Saccharomyces cerevisiae, the transcriptional repressor Nrg1(Negative Regulator of Glucose-repressed genes) and the β-Ziptranscription factor Rtg3 (ReTroGrade regulation) mediate glucose repression and signaling from the mitochondria to the nucleus, respectively. Here, we show a novel function of these, two proteins, in which alanine promotes th...

Poultry farming, raising of birds domestically or commercially, primarily for meat and eggs but also for feathers. In poultry industry, probiotics applications have widely been shown to improve the barrier function of intestine and reduce pathogenic problems in gastrointestinal tract thus leading to the enhancement of immune response and replacemen...

Background The gut mycobiome of patients with lung adenocarcinoma (LUAD) remains unexplored. This study aimed to characterize the gut mycobiome in patients with LUAD and evaluate the potential of gut fungi as non-invasive biomarkers for early diagnosis. Methods In total, 299 fecal samples from Beijing, Suzhou, and Hainan were collected prospective...

This study investigates the potential of Tacca leontopetaloides starch as an alternative raw material for bioethanol production. Ragi tapai was used as the saccharification and fermentation agent and different origin of ragi tapai was evaluated for the highest glucose production. Ragi tapai from Sabah (RTSab) produced significantly higher glucose a...

Comprehensive yeast strain characterization is an important issue for the wine industry as market demands require controlled production of distinctive high-quality wines. Glycosides form an important reservoir of varietal grape wine aroma, and their hydrolysis into olfactory-active compounds essentially depends on the fermenting yeast genera and st...

Yeast Saccharomyces and its derivatives have been largely used in livestock and poultry nutrition for their potential positive impact on growth, performance, and general health. Originally included in animal diets as a source of protein, yeasts can also offer a wide range of by-products with interesting bioactive compounds that would confer uses be...

Microflora play an important role in the fermentation of blueberry wine, influencing the flavor and nutrient formation. Commercial yeasts give blueberry wines an average flavor profile that does not highlight the specific aroma and origin of the blueberry. In the present study, ITS1-ITS2 region sequencing analysis was performed using Illumina MiSeq...

Assaying changes in the amount of DNA in single cells is a well-established method for studying the effects of various perturbations on the cell cycle. A drawback of this method is the need for a fixation procedure that does not allow for in vivo study nor simultaneous monitoring of additional parameters such as fluorescence of tagged proteins or g...

Koumiss, a traditional fermented dairy product made from fresh mare milk, is a sour beverage that contains an abundance of microbial communities, including lactic acid bacteria, yeast and others. Firstly, probiotics such as Lacticaseibacillus in koumiss can induce the secretion of immunoglobulin G in serum and interleukin-2 in the spleen while bene...

Propolis is a natural substance that has anti-inflammatory (immunomodulatory and microbicidal) properties and has a long history of use in traditional medicine. The aim of this study was to evaluate the antifungal properties of 10% and 30% ethanolic extract of propolis prepared traditionally and of a commercial drug metronidazole, against selected...

Contamination of locally formulated herbs with aflatoxins and pathogenic organisms poses major health concerns to humans and animals, especially in recent times when herbal mixtures are on the rise. This study aimed to determine the herbs' microbial profile and aflatoxin level. Two different herbal medicines (malaria and typhoid; each prepared with...

Wine is a fermented product of fruit juice. Mulberry and apple juice can also be used to produce wine. Several factors influence the alcoholic fermentation of the must; therefore, the optimization of the mulberry wine fermentation conditions with the dry yeast strain Saccharomyces bayanus (concentration varied from 0.15 to 0.25 g/L), fermentation c...

To understand the absence of Pergularia tomentosa L. (Apocynaceae) in the Mauritanian pharmacopoeia, we characterized the phytochemical composition, evaluated its biological and antioxidant activities. The phytochemical study shows the presence of flavonoids, tannins, saponins and steroids in the roots and aerial parts. Alkaloids, heterosides and t...

Background Since DNA information was first used in taxonomy, barcode sequences such as the internal transcribed spacer (ITS) region have greatly aided fungal identification; however, a barcode sequence alone is often insufficient. Thus, multi-gene- or whole-genome-based methods were developed. We previously isolated Basidiomycota yeasts classified...

Background Pet cats frequently have diarrhea in their daily life. Bacillus has a protective role that has crucial beneficial functions on intestinal homeostasis. The aim of this research was to investigate the effects of the compound Bacillus on the prevention of diarrhea, microbiota and metabolism in pet cats. A total of 20 pet cats (1–2 years old...

The inebriation of wild African elephants from eating the ripened and rotting fruit of the marula tree is a persistent myth in Southern Africa. However, the yeasts responsible for alcoholic fermentation to intoxicate the elephants remain poorly documented. In this study, we considered Botswana, a country with the world's largest population of wild...

Transcription factors (TFs) play key roles in cellular regulation and are critical in the control of drug resistance in the fungal pathogen Candida albicans . We found that activation of the transcription factor C4_02500C_A (Adr1) conferred significant resistance against fluconazole. In Saccharomyces cerevisiae , Adr1 is a carbon-source-responsive...

In the present study, the prepared bio-composite materials based on flax fibers containing caffeine or nano-copper (Cu) or corundum (Al 2 O 3 ) as a potential biocidal substance were prepared and tested on their toxicity. Two viability tests were performed. Firstly, the screening test based on colouring by Methyl-blue was applied on yeast cells of...

The textile industry is one of the largest industries in the world and contributes positively to the economic growth of a country. However, the improper disposal of textile dye effluents results in discharging of high concentrations of textile dyes into the environment. Moreover, the inherent stability of textile dyes makes them hard to remove from...

Water-extractable arabinoxylan (WEAX) may cause major problems during clarification processes in a brewery owing to its ability to form gel networks. However, high WEAX contents can also enhance the nutritional quality of the final product as they play an important role in the human diet. Therefore, precise quantification of WEAX is required. Curre...

As a life-essential coenzyme, nicotinamide adenine dinucleotide (NAD+) has been explored for more than a century. In Saccharomyces, the natural NAD+ de novo biosynthetic pathway initiating from tryptophan has been well elucidated. To bypass this stringently controlled natural pathway in yeast, an economical C3N pathway that was developed in Escheri...

Autophagy is one of the main degradative pathways used by eukaryotic organisms to eliminate useless or damaged intracellular material to maintain cellular homeostasis under stress conditions. Mounting evidence indicates a strong interplay between the generation of ROS and the activation of autophagy. Although a tight redox regulation of autophagy h...

Tiger nut (Cyperus esculentus) milk beverage is a nutritive, energetic and popular drink mostly produced and consumed in Northern Nigeria. However, the beverage has poor shelf-life which hampered its large-scale production and profitability. Big yellow tiger nut was soaked in 3 L of tap water at 60 o C for 6 hours, additives such as coconut, date a...

Aging is characterized by a number of hallmarks including loss of mitochondrial homeostasis and decay in stress tolerance, among others. Unicellular eukaryotes have been widely used to study chronological aging. As a general trait, calorie restriction and activation of mitochondrial respiration has been proposed to contribute to an elongated lifesp...

Theanine is a non-protein amino acid that is highly represented in tea plants and is one of the delicious ingredients in tea. In recent years, the global market demand for theanine has continued to rise, and the industry has developed rapidly. Here, we designed and constructed a promising pathway in Escherichia coli to produce L-theanine. This bios...

This study examined the effect of simultaneous fermentations of Saccharomyces cerevisiae and Pichia kluyveri, Lactobacillus plantarum and Oenococcus oeni on the chemical composition of apple cider from two apple varieties—Topaz and Red Topaz. Analytical techniques (HPLC-RID, HPLC-VWD, GC/MS, GC/FID, HPLC-DAD ESI+) were employed to analyze glucides,...

Caffeine is a verified bio-protective substance in the fight against the biodegradation of cellulose materials, but its ecotoxicity in this context has not yet been studied. For this reason, the ecotoxicity of flax-fiber-reinforced epoxy composite with or without caffeine was tested in the present study. Prepared samples of the composite material w...

We report draft genome sequences for 15 non-conventional Saccharomycotina yeast strains obtained from public culture repositories. Included in our collection are eight strains of Pichia with broad tolerance to dicarboxylic acids. The genome sequences of these strains will enable comparative genomics of acid-tolerant phenotypes and strain engineerin...

Changes in lifestyle and the negative impact of chemicals make people aware of the dangers of consuming food products containing chemical residues so many people switch consuming organic food. Black rice is one of the kinds of rice that is widely consumed as an organic functional food because of its antioxidant and dietary fiber content. Black rice...

Objectives The present work aimed to develop a new method for rapid extraction of the fungal genome for Polymerase Chain Reaction (PCR). Results(method) We used only 15 minutes to obtain an intact genome for PCR, due to cells were disrupted in the 0.5 mol/L NaOH solution. The quality and quantity of genomic DNA was verified by Nanodrop under OD260/...

Persimmon leaves usually as agricultural and forestry waste were fermented by Lactiplantibacillus plantarum and Saccharomyces cerevisiae. Growth and metabolic performances of L. plantarum and S. cerevisiae, as well as the effect of fermentation on the antioxidant abilities of the extract was investigated, including the content of flavonoids, 2,2-Di...

This study aimed to explore key physicochemical characteristics and evolutionary patterns of microbial community structure during the fermentation of aged vinegar. The correlation between microorganisms and physicochemical characteristics during fermentation was examined. The results revealed significant differences in genera at different stages of...

Lianhua-Qingwen (LHQW) stands as a compound herbal remedy renowned for its efficacy in addressing pandemic viral ailments like COVID-19. The production of LHQW capsules, however, generates substantial residues, contributing to environmental strain and resource squandering. To counteract this, we have devised a meticulously orchestrated co-fermentat...

Fermentation vessels affect the characteristics of food fermentation; however, we lack an approach to identify the biomarkers indicating fermentation. In this study, we applied metabolomics and high-throughput sequencing analysis to reveal the dynamic of metabolites and microbial communities in age-gradient fermentation vessels for baijiu productio...

Polycystic ovary syndrome (PCOS) is a serious disease characterized by high androgen, insulin resistance (IR), hyperglycemia, and obesity, leading to infertility. The gut mycobiota has been reported to evolve in metabolic diseases including obesity, hyperglycemia, and fatty liver. However, little is known about the gut mycobiota and PCOS. In the cu...

Although molybdenum trioxide nanomaterials have been widely explored as nanoagents for biomedical applications against bacteria through photothermal therapy, chemodynamic therapy, and catalytic therapy, their utilization as photosensitizers for photodynamic therapy (PDT) have been rarely reported so far. Herein, we report the activation of MoO 3 na...

Background: Primary sclerosing cholangitis (PSC) is a chronic, fibroinflammatory, cholestatic liver disease of unknown etiopathogenesis, often associated with inflammatory bowel diseases. Recent evidence ascribes, together with immunologic and environmental components, a significant role to the intestinal microbiota or its molecules in the PSC pat...

Microflora play an important role in the fermentation of blueberry wine, influencing the flavor and nutrient formation. Commercial yeasts give blueberry wines an average flavor profile that does not highlight the specific aroma and origin of the blueberry. In the present study, ITS1-ITS2 region sequencing analysis was performed using Illumina MiSeq...

Saccharomyces cerevisiae is commonly used for the production of alcoholic beverages, including cider. In this study, we examined indigenous S. cerevisiae and S. uvarum strains, both species commonly found in cider from Hardanger (Norway), for their strain-specific abilities to produce volatile and non-volatile compounds. Small-scale fermentation of...

Examination of the genome sequence of Saccharomyces cerevisiae strain S288c and 93 additional diverse strains allows identification of the 5873 genes that make up the core set of genes in this species and gives a better sense of the organization and plasticity of this genome. S. cerevisiae strains each contain dozens to hundreds of strain specific...

Certain microorganisms are capable of proliferating in wine despite its low pH and high ethanol content. The yeasts of the Saccharomyces genus responsible for alcoholic fermentation can alter wines with residual sugars; the proliferation of Brettanomyces bruxellensis brings about thoroughly unpleasant sensory changes. The main strategy currently ap...

Most communities in the oil rich Niger Delta find it difficult to access to potable water due to constant pollution of their natural water sources. The sole aim of this study was to examine the microbiological and physicochemical qualities of the Estuary water at Utaewa to ascertain its suitability for potable use. Standard culture dependent techni...

Medicinal and edible seed Semen Persicae is susceptible to mycotoxin and fungal contamination. However, the occurrence of mycotoxin contamination and fungal infection is still unclear. In this paper, ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry and high-throughput sequencing were conducted to determine the...

Microbial activity during spontaneous fermentation in alcoholic beverages have driven in developing the chemical and aromatic characteristic of products but not clear in apricot wines. We have characterised the composition of fungal communities and volatile metabolites in apricot wine spontaneous fermentation among two Shaanxi regions. Results show...

Introduction Sensitive scalp is one of the most frequent complaints related to sensitive skin syndrome, characterized by unpleasant sensory reactions in the absence of visible signs of inflammation. In this study, the effects of topical application of postbiotic Himalaya-derived Saccharomyces and Lactobacillus ferment complex (SLFC) on the bacteria...