Science topics: RosalesRosaceaeRosa
Science topic

Rosa - Science topic

A plant genus in the family ROSACEAE and order Rosales. This should not be confused with the genus RHODIOLA which is sometimes called roseroot.
Questions related to Rosa
  • asked a question related to Rosa
Question
3 answers
thanks
Relevant answer
Answer
It is difficult to determine what thermal conductivity you are trying to measure based on your explanation and diagram. However I believe I can help with some of your questions. Since you only have one resistive load in your circuit you will always see your full generator voltage across the load. If the temperature changes resulting in a change in resistance in the sensor you will see a change in the current. For example if the sensor increases in resistance with an increase in temperature your current will lower as temperature increases.
Normally the temperature is read using a bridge circuit. This link is a good description of how bridge circuits operate: https://www.processtechacademy.com/instrument-tech-must-knows-rtd-bridge-circuits/
Measuring thermal conductivity is a bit more difficult. Thermal conductivity is the rate at which heat is conducted through a material. This is measured by arranging the material under test such that a low temperature is placed on one side with a higher temperature source is placed on the other side. The change in temperature on the low side is monitored until the temperatures equalize. When the temperatures are plotted the slope of the line shows the thermal conductivity.
A more detailed explanation of this process can be found at https://www.wikihow.com/Measure-Thermal-Conductivity.
I hope this helps, Paul
  • asked a question related to Rosa
Question
1 answer
Hi,
Is there a cell line Rorc gamma T positive that I can use to perform assays in vitro?
Thanks!
Rosa
Relevant answer
Answer
It will be helpful if you provide the Gene ID for the gene. But if it is RORC gene, these resources may be helpful:
From these, it seems HDLM-2 is a good candidate.
They are transcript data, so depending on your purpose you may wish to validate that the protein is well-expressed (immunoblot).
Cell lines which show protein expression are:
  • asked a question related to Rosa
Question
6 answers
Common Rose-Pachliopta aristolochiae individuals seen flying one be one over sea shore more than twenty kilometers in a stretch was able to watch. This phenomena observed around one month from December last to January last 2022. To and fro flight observed. Some dead specimens were observed near the sea shore.Why they engaged in these continuous flight? Any one can give answers?
C.Susanth Kumar
Kerala,India.
Relevant answer
Answer
Hello Susanth; In Southern California on breezy days, some species of butterflies are seen "hill-topping'. That is, they fly in place in exposed places. They are engaged in mating aggregations. Maybe you saw something like that. Best regards, Jim Des Lauriers
  • asked a question related to Rosa
Question
4 answers
What is the role of different iron sources in the commercial tissue culture of roses?
Relevant answer
Answer
Dear Mahipal Shekhawat it depends of plant species, and the goal you're trying to reach as well. Under my conditions, using FeEDDHA instead FeEDTA the multiplication rates, the growth of microshoots, the plant quality, the rooting, and survival were improved considerably. However, I don't obtained any observable advantage doing the same with wild cherry and garlic. You must also to consider that Van der Salm et al. (1994) demonstrated that FeEDDTA is break down under light conditions, causing chlorosis because of iron deficiency.
  • asked a question related to Rosa
Question
3 answers
I am creating a Rose plot from the joint data from a site, and I was using Dips. But It appeared not that clear to me, I used Dip direction and Dip amount as an input. Then I used Python Library mplstereonet to generate from same data and got different information. If any one can elaborate the issue will be highly obliged. Or are there better method to solve the issue ?
Relevant answer
Answer
Great question, thanks for sharing.
  • asked a question related to Rosa
Question
4 answers
Dear Plant Tissue Culture Experts,
Many crop and horticultural plants are produced by commercial tissue culture laboratories all over the world such as bananas, strawberries, sugarcane, etc. But many important medicinal plants could not be possibly propagated via tissue culture techniques at a mass scale, like Sandal, etc.
I want to know whether the commercial tissue culture of Rose is possible?
If yes, what are the protocol and PGRs? If not then why is it so?
Whether Rose has any different kinds of nutritional or physicochemical requirements under in vivo or in vitro conditions?
Relevant answer
Mahipal Shekhawat of course. A lab in Bulgaria, settled in the Rose Valley (Kazanlak), micropropagate commercially various rose accesions.
Here is the link
  • asked a question related to Rosa
Question
3 answers
Hi all,
I am carrying out some IF to check immune cells in intestine. Some of these cells should be labelled with GFP so I want to do a co staining with another marker to specific identify this population. However, I don't get a good GFP signal... so how could I improve? I was also looking for publications but I have not found more so far...
Bwt IF is performed on paraffin embedded tissue (intestine) and I used GFP primary Ab 1:300 with amplification (streptavidin-biotin)
Thanks!
Rosa
Relevant answer
Answer
I don't have any other ideas.
If you have live mice, you could use frozen sections for the experiment.
  • asked a question related to Rosa
Question
4 answers
I operate a bioreactor at 50 Celsius, whose heating system failed. Upon fixing the heating system within the next couple of days after failing, I noticed that there is a quite a bit of water that the reactor with drew from water displacement reservoir (to measure volume of biogas produced) the next day. From what I interpret, when the heating system kicked in, the temperature rose from 25 C to 55C, which might have resulted in expansion of the inside the bioreactor and then drew water into the reactor to equilibrate.
Also, I tried removing the excess liquid from bioreactor, and when I did, reactor draws more water as I remove, to maintain its levels. More sort of a level controller based on pressure.
I did fix all the problems now after troubleshooting. I am trying to understand the science/ principle and mechanism behind all this. I'd appreciate your insights on this!
Note: After water entering the reactor, level rose to a point where the tip of the tube connected to water displacement reservoir touches the liquid level.
Relevant answer
  • asked a question related to Rosa
Question
1 answer
Comments on An efficient method for mapping the 12C+ 12C molecular
resonances at low energies
Xiangqing Yu1,*
1Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
The goal of this reply is to draw attention of the readers that the major problems rose in the paper authored by X. Tang et al. [1], this is a brief analysis of the paper [1], there is some inconsistency in the paper [1], there are some errors in the paper [1], and there are some groundless exaggerated boasts in the paper [1].
The paper of 'An efficient method for mapping the 12C+ 12C molecular resonances at low energies' was published in NUCL SCI TECH [1], but there are some problems and shortcomings in the paper.
The author [1] wrote that nuclear structural research, but as far as I know, Nuclear structural research is usually written as nuclear structure research or nuclear structure study, Nuclear structural research may be a kind of innovation in nuclear structure research, is not it?  
The author wrote that molecular resonance in the Keywords, but there are no exact study results that address the molecular concept resonance and there are no the right experimental methods to detect the molecular resonance in the whole paper. The 12C+ 12C molecular resonances represent true cluster states in the 24Mg compound system, or whether they simply reflect scattering states in the ion-ion potential [2], and the cluster physics in the Wikipedia is described as small, multiatom particles. As a rule of thumb, any particle of somewhere between 3 and 3×107 atoms is considered a cluster [3], the cluster is no less than 3 nucleons in the Wikipedia [3], while in the paper [1] the authors just measure the proton which contains 1 nucleon. If all the residual nuclei which contain no less than 3 nucleons in the nuclear reactions could be treated as molecular, then all the resonances of the residual nuclei which contain no less than 3 nucleons in nuclear physics can be treated as molecular resonances, isn't it ridiculous?
In the reference [4]E. Almqvistet et al. wrote that the results strongly suggest spins 2 and 4 for the "quasimolecular" states at 19.5- and 19.9-MeV excitation in Mg24 , The partial widths of these states reduced to units of📷appropriate to each channel are given; in these units the C12 width is more than ten times the average width for α-particle emission and one hundred times the average nucleon width. In the reference [5]The nucleus 8Be has been conjectured to resemble a molecule of two interacting α-particles. A crucial test of this conjecture is the electromagnetic transition between the molecular resonances. This paper discusses the earlier indirect bremsstrahlung measurements and describes a recent experiment on the direct measurement of γ-transition between the 4+ and 2+ resonances, while the author [1] wrote that Each detector was covered with a 12.7-📷m-thick Al foil in the front to completely stop the α particles emitted from the 12C(12C,α)20Ne reaction, if the α particles was completely stopped, then all then ejectiles whose mass are heavier than α particle could also be completely stopped for the stop power of the ejectiles, there are only particles whose mass number are less than α particle mass number in the energy spectrum of Fig. 5 [1], in this experimental method [1] how to determine the C12 width as molecular resonances without gamma ray detection? The correct experimental method to detect the molecular-like resonance is detect the 12C molecular as a integral cluster
or the correct experimental method to detect the gamma or other signal of the excited states of the 12C, so the red mark of 'Resonance' of molecular resonances in the energy spectrum of Fig. 5 [1] could not be proved by compared with previous references or other methods, while red mark of 'No resonance' in the energy spectrum of Fig. 5 [1] is a real resonance, otherwise why the highest energy of proton is less than 2.5 MeV? If the true p1 events were distributed in the range from 7 to 8.2 MeV in the energy spectrum of Fig. 6 [1] is correct, then why is the highest energy of proton is about 5 MeV or more than 5 MeV in Fig. 3 [1], why is that? These are phenomena of logical contradiction, so there are no exact study results that address the molecular resonance in the whole paper, the author just use the the expression of molecular resonances to catch our eyes even he does not know what is the exact physical meaning or even he does not know what is the exact detecting method of the molecular resonances.
In the reference [6]T. Spillane et al. wrote that the data exhibit a pronounced resonance structure down to our low-energy limit, where a strong resonance is found at ER = 2138 ± 6 keV (width 📷< 12 keV) , if we could not trust T. Spillane's experimental result and others, then what will be trusted by us? and if we could trust T. Spillane's experimental result and others, then the results of the paper [1] are not correct because the experimental method of the paper [1] is to detect the proton while the experimental method of T. Spillane's paper is to detect gamma ray. There is no any resonance phenomenon at ER = 2138 ± 6 keV with so called thick target method in the result of the paper [1], but there is a strong resonance phenomenon is found at ER = 2138 ± 6 keV in T. Spillane's result, the author [1] wrote that the 12C(12C, p)23Na reactions were measured by experiments in the center of mass energy range of 3-5.3 MeV using thick targets. The author also wrote an infinitely thick target (i.e., thickness much greater than the beam range inside the target material) is used in this method, but the author wrote two contradictory descriptions, one description is the center of mass energy range of 3-5.3 MeV, and the other description is the center of mass energy range of 0-5.3 MeV for an infinitely thick target, It is now clear that the efficient thick target method outlined in the present work [1] will not be useful in searching for potentially existing molecular-like resonances of 12C+ 12C or resonances of 12C(12C, p)23Na in the energy range 1 MeV<Ecm<3 MeV, because there are not any proton resonance peaks or other useful signals beween the energy range 1 MeV<Ecm<3 MeV in the energy spectrum [1].    
  The author [1] wrote that to precisely map the resonant structures, fine energy steps (e.g., 📷<100 keV in the laboratory frame) are required. The yield difference between two adjacent energy points Y(E) and Y(E-📷) is calculated to determine the dY/dE. The author also wrote therefore, a range of reaction energies [Ebeam-📷E, Ebeam] is scanned with a single, constant beam energy. The effective width of the scan, 📷E, usually spans from 500 to 800 keV, but the author wrote two contradictory descriptions, although there is some difference between the 📷 energy in the center-of-mass frame and the 📷E in the laboratory frame, the 📷E =500 keV is the same as 📷=250 keV, is 250 keV smaller than 100 keV? isn't this ridiculous?
  The author [1] wrote that the reaction Q-value spectrum is computed from Eq. 2 [1] with a constant incident energy of Ea =8.2 MeV, I think the Q-value can be calculated with the already known nuclear data, and that the reaction Q-value spectrum is computed from Eq. 2 [1] with a constant incident energy of Ea =8.2 MeV is a repetitive work of no great significance in the 12C(12C, p)23Na reaction, is it exaggerated boast or does the author shuffle up a paper with little self-confidence? The author [1] wrote that the Q-value spectrum, and the Q-value spectrum concept is not suit to be utilized here [1] because the Q-value of the reaction channel 12C(12C, p)23Na is a fixed value, a fixed value is not much wider spectrum or simple sharp Gaussian shape spectrum [1]. The author [1] wrote that the Q-value spectrum obtained with a thin target is expected to be narrowly 1.80 MeV, it is not narrowly or narrow 1.80 MeV, it is a fixed value of 1.80 MeV which can be calculated with the already known nuclear data.
   The author [1] wrote that the p1 channel events obtained with the thick target form a wide Q-value spectrum when we compute the Q-value using a fixed beam energy, Ea =8.2 MeV, is it funny?
   The author [1] wrote that the Q-value of the p0 channel also has a low-energy tail, similar to the p1 channel. These low-energy events from the p0 channel interfere with the high-energy events from the p1 channel. As shown in Fig. 5 [1]. As we know there are some necessary conditions of wave interfere phenomena, the wave length of matter-wave [7] is too short for heavy matter, if the wave length of matter-wave [7] was shorter than the matter size, then it is usually difficult to observe the wave-like characteristics,  and the author [1] can not say that the matter-wave of earth interfere with the matter-wave of moon, the author [1] must prudently say that the matter-wave of superheavy nucleus interfere with the matter-wave of another superheavy nucleus, and the author [1] must prudently say that the matter-wave of proton interfere with the matter-wave of another proton if they could not have the same frequency or very similar frequency, obviously there is dominant superposition of p1 energy spectrum and p0 energy spectrum, which is not dominant interfere phenomena, the reason is that the wave length of 5 MeV proton is about 1.28📷10-14m, the wave length of 2.2 MeV proton is about 1.93📷10-14m, the wave length of 1.6 MeV proton is about 2.27📷10-14m, while as we know the size of proton is about 8.4-8.7📷10-14m, the length of matter-wave of proton is smaller than the size of proton in the paper [1], so it is usually difficult to observe the wave-like characteristics, then author [1] wrote that 'the Q-value of the p0 channel also has a low-energy tail, similar to the p1 channel. These low-energy events from the p0 channel interfere with the high-energy events from the p1 channel.' is wrong.
  I do have my doubts about only the first author Xiao-Dong Tang and the corresponding author Xiao Fang know the process of posting a paper [1], but whether do other authors really know that send an article to a the magazine NUCL SCI TECH [1] or post a paper in the journal NUCL SCI TECH [1], it is really to be queried, and it can be proved by the expression of the correspondence author: akram@comp.tamu.ed, the correct expression is author for correspondence or the correct expression are other expressions.
In the reference [8] although the first authour is A. M. Mukhamedzhanov, as far as I know X. Tang who act as an organizer and pusher of posting a new comments paper, and as far as I know X. Tang had found D. Y. Pang for a help to write the comments [8], and then A. Tumino et al. come on Reply to the Comments on the 12C+12C fusion S*-factor, but it is strange that I can not search the author [1] Xiao-Dong Tang i.e. the author of the reference [8] the organizer X. Tang's Reply to Reply to the Comments on the 12C+12C fusion S*-factor, why?
📷
[1] Xiao-Dong Tang et al.,An efficient method for mapping the 12C+ 12C molecular resonances at low energies. NUCL SCI TECH 30, 126 (2019).
[2] C. Beck, Molecular resonance phenomena and alpha-clustering: recent progress and perspectives. https://arxiv.org/pdf/nucl-ex/0401004.pdf.
[4] E. Almqvistet et al., Spins and Partial Widths of Quasimolecular Resonances in C12+C12 Interactions. Phys. Rev. 130, 1140(1963).
[5] D R Chakrabarty, Electromagnetic transition between molecular resonances in 8Be. Pramana 83(5), 635-642(2014).
[6] T. Spillane et al., Study of the 12C+12C fusion reactions near the Gamow energy. Phys.Rev.Lett. 98, 122501(2007).
[7] Uwe Becker, Matter-wave interference made clear. Nature 474, 586-587(2011).
[8] A. M. Mukhamedzhanov et al., Comments on the 12C-12C fusion S*-factor. https://arxiv.org/pdf/1806.05921.pdf.
[9] A. Tumino et al., Reply to the Comments on the 12C+12C fusion S*-factor.  https://arxiv.org/pdf/1807.06148.pdf.
Relevant answer
Answer
Is there some consistency in X. Tang's paper?
  • asked a question related to Rosa
Question
6 answers
I would to assess the urban growth process on multiple directions (rose diagram) in order to be able to generate some statistics like in the attached paper. Could you recommend the methodological steps for this type of analysis?
Relevant answer
Answer
At first, you need a software called Rockwork, through which you can able to plot the orientation of different direction. Before that, you can use Splitline option in ArcGIS to find the X & Y direction.
  • asked a question related to Rosa
Question
4 answers
In the recent years, the authoritarian governments have increasingly rose to power in major economies around the world. The adoption of policies favouring domestic employment as well as local producers casts doubts on the future of globalization. In this regard post your opinion.
Thank you
Relevant answer
Answer
In my opinion, information and technological globalization will dominate in the future, at least in the next few years. On the other hand, economic globalization may in some respects diminish its importance. For example, international intermodal supply and distribution logistics chains may be shortened. The SARS-CoV-2 (Covid-19) coronavirus pandemic initiated structural changes in the globalization processes that will be continued in the coming years.
Regards,
Dariusz Prokopowicz
  • asked a question related to Rosa
Question
6 answers
The majority of damask rose flower in spring, but some varieties bloom in autumn. Do you know them? What are their origin? 
Relevant answer
Answer
hi
we can't recognize easily. we have to consider morphological characters and compare with taxonomy key. but , usually, these roses are fragrant, and also dried petal save their smell. if you extract essential oils of them, you can recognize according to components of essence.
  • asked a question related to Rosa
Question
2 answers
Sometimes a bending of the flower stalk of the rose plant occurs when it bears large flowers, especially thin stalks.
Relevant answer
Answer
Bending is important for keeping enough leaves on plant and maintain balance between source and sink. A minimum of four stems have to done from each plant and is advisable to do in the afternoon and create two 45 degree bends below the horizontal. Bottom breaks will appear after bending which will carry the production of flower buds.
  • asked a question related to Rosa
Question
2 answers
How to dilute Modified Citrus Pectin ( EcoNugenics, Santa Rosa CA, USA) in the water? I have tried different methods such as heating and dissolving with PBS, DMSO or alcohol. But I even could not get 1% MCP solution (0.1 gram MCP in 10 mL water. It was not completely dissolved that you could see sediment at the bottom).
Relevant answer
Answer
Hello, Mr. Shao. My lab colleague has the same problem with MCP and inulin. Dissolving does not appear to be complete for this Polysaccharides. However, studying these two cases helped to make progress in the work:
Reduce the concentration used in work (for example, using 70 mg MCP in 10 ml water). Place the solution with a magnetic stirring bar on a hot plate stirrer at 80° C and raise the pH of the solution using NaOH 2N to dissolve slowly. It takes a long time for the resulting solution to remain stable and less precipitate/gelatinize. Other things worth trying (Of course I'm not sure): Citric acid at 90 °C for 90 min at a ratio of 1:80 (w/v). (?!) Using an ultrasonic bath and Fenton reaction for better mixing.
  • asked a question related to Rosa
Question
3 answers
Anyone please help me to have some article, related to the whole post harvest handling process for rose loose flower.
Relevant answer
Answer
Karun Katoch and Nataraj S K sir , thank you for sharing the information.
  • asked a question related to Rosa
Question
18 answers
Do you prefer raising birds or planting roses? Why?
Relevant answer
Hi Maysoon Al-Hadraawy
I prefer planting roses, because it's colors and weaving give us hope and making our hearts rejoice.
Best Regards
  • asked a question related to Rosa
Question
2 answers
There are some chemical materials used for the abscission of fruits (for example Ethephon). This materials may have destructive effects on the essential compositions of roses. Now, I am questing to find a way to separate the sepals from petals of rose flowers by mechanical means.
Additionally pleases I would like to know if there is any other chemical materials with out destructive effects on the essential compositions.
Relevant answer
Answer
In my openion it is physiological process are you trying to detach the petals of oil bearing species or cut flower varieties bcz oil bearing species petals will be spreading and few in number, study reveals that highest essential oil content will be more at complete open stage. Obviously Hybrids and cut flower s more number of petals. So do let me know which type of flowers you r looking
  • asked a question related to Rosa
Question
4 answers
Dear scholars,
I decide to deposit rose gold coat by using cathodic arc pvd. my target is Ti and the gas is acetylene, nitrogen and argon. I really want to know the deposition parameter and method since I concern about acetylene reactivity and the danger of its explosion. I am so grateful for your guides.
Saba Khoram
Relevant answer
Answer
1-Generally for the film color you should consider its thickness. Also you should check how much your PVD technique is homogeneous. When you look at your samples, do they present the same color all over the surface? If so then your technique is homogeneous. If you see rainbow colors then it is not. Generally, PVD is more homogeneous than PECVD over an average surface.
2-According to my knowledge, golden shades mean low thickness and you start to see blue, reddish and green shades when you increase the thickness. So I think 3 min should be enough at this bias voltage. Colors also depend on films microstructures and refractive index. I have never worked with acetylene to tell you, but for nitrogen, the risk of using high flow remains in the disorder that you could generate in your film. A disorder means refractive index variation.
As for acetylene, you should of course stay in the safe flow ranges or find a safer carbon source (if possible).
Regards
Loraine
  • asked a question related to Rosa
Question
3 answers
I would like to know if it is possible to?
Relevant answer
Answer
I agree with @Peter Farrington
  • asked a question related to Rosa
Question
3 answers
I am attempting to synthesize a cosmeceutical facial spray that is an o/w emulsion. Below is my prototype formula using Polysorbate-80 and Polysorbate-20 as emulsifiers, Leucidal and Linatural as preservatives and Disodium EDTA as a chelating agent and preservative booster. I was hoping to get feedback on my formula to see if it seemed stable on paper. I have approximately a 1:1 ratio of oil:emulsifier, a 3% final volume of preservative with a chelating booster.
• Rose water 33% pH ~5.5 (99mL)
• Purified water 33% pH ~7 (99mL)
• Jojoba seed oil 5% pH ~5 (15mL)
• PolySorbate80 5% pH ~6 (15mL)
• Glycerin, palm 4% pH ~6.5 (12mL)
• Argan oil 3% pH ~5 (9mL)
• Licorice Root extract 3% (9mL)
• Aloe extract 3% pH ~5 (9mL)
• PolySorbate20 3% pH ~5.5 (9mL)
• Dimethicone 3% (9mL)
• Leucidal 2% (6mL)
• Linatural 1% (3mL)
• Geranium Rose oil 0.5%(1.5mL)
• Disodium EDTA 0.5% (15mG)
• Jasmine extract 0.3% (1mL)
Water Phase (all ingredients introduced while sheering and sheered until solute): Gently heat water + rose water dilution to 70C. Add Disodium EDTA. Add glycerin. Add Aloe extract. Add Licorice Root extract. Add polysorbate80. Add Polysorbate20.
Oil Phase (all ingredients introduced while sheering and sheered until dispersed; final oil phase mixture heated to 70C): Add jojoba oil. Add argan oil. Add dimethicone.
Emulsifying Phase: Slowly add water phase to oil phase while sheering until 50% of water phase is added, then quickly add remaining 50% of water phase. Remove from heat and sheer for 1 hour.
Additive phase: Add Geranium Rose extract, sheer. Add Jasmine extract, sheer. Add Leucidal, sheer. Add Linatural, sheer. Store final emulsion in sterile airtight, UV protectant bottles.
Relevant answer
Answer
Except for theoretical surface tension calculations, a lot of work depends on experimental optimizations.
Particle distribution should also be considered (measurements).
  • asked a question related to Rosa
Question
57 answers
Decades of rising emissions continued to do what scientists have long warned they would: make the world hotter.
Indeed, the 2010s mark the decade when the impacts from climate change became unmistakable, at least for any objective-minded observer.
Carbon dioxide from fossil fuels, which makes up about 90% of total emissions from human activities, was relatively flat from 2013 through 2016.
However, Fossil-fuel emissions rose an estimated 0.6% to a record 37 billion metric tons in 2019, capping three straight years of growth.
Sea-level rise is accelerating!!
The planet got a lot hotter this decade!!
Most countries have done very little so far to displace the power plants, cars, factories, and buildings polluting the atmosphere with more emissions each year.
In your view, is the world doing enough on climate change?
Relevant answer
Answer
I do not believe it is. Many people and companies are seeing the signs and disregarding them.
  • asked a question related to Rosa
Question
5 answers
Dear all,
I am looking for a good course (online) training in Experimental Design. Under you opinion which should have a good quality?. Thank you in advance,
Kind regards
Rosa
  • asked a question related to Rosa
Question
3 answers
For Rosa damascene micropropagation, Use of Which Growth Regulators is to Increase the Number of Shoots Extracted from the Stem Node?
Relevant answer
Answer
Hello
Appropriate growth regulators treatment depends on some factors such as accession, growth conditions and mother plant age. In the articles, combination of 2.5–3 mg/l BA + 0.1 mg/l IBA, 3 mg/l BA + 0.1 mg/l IAA, 5 mg/l BA + 0.1 mg/l TDZ, 2 mg/l BA + 2 mg/l GA3 and 1-2 mg/l BA + 0.1 mg/l GA3 ± 0.1 mg/l NAA were the most suitable treatment for proliferation.
  • asked a question related to Rosa
Question
6 answers
Here, at IFC campus Santa Rosa do Sul, Brazil, we are interested and testing agriculture treatments with green synthesis of silver nanoparticles in order to prevent plant diseases.
But we thinck it's necessary to obtain a high concentration of suspension for pratical purposes. What kind of product are you doing, his concentration and dilution for reading at the spectrophotometer? How you determinate the conversion?
Thanks.
Relevant answer
Answer
We use a low concentration for gold nano or clay that give excellent results as mentioned in both patents to get red or prevent the virus particles as a contagious pathogen in the plants
  • asked a question related to Rosa
Question
2 answers
I would like to create a plasmid map for a poster presentation. The plasmid is the pUC57-Kan­­­­­­­­ plasmid containing a mini-CMV promoter, IL-2 gene, SV40 poly(A), and a mouse ROSA 26 origin of replication. I have the sequence for the IL-2 gene and was able to find the backbone sequence for pUC57-Kan on Addgene. I have no other information. How should I go about making plasmid map with the information I have?
Relevant answer
Answer
You can use free trial version of SnapGene and simulate for example restriction cloning putting the sequence of the backbone plasmid pUC and your insert IL-2.
  • asked a question related to Rosa
Question
4 answers
I could like to know the best bio-pesticide or the best biological (IPM) method that can be applied in the control of botrytis in roses .
Relevant answer
Answer
Endophytic microorganisms are the main cause.
Dr Kamath Madhusudhana
  • asked a question related to Rosa
Question
1 answer
Hi
I need Articles about effect of calcium on rose production
Relevant answer
Answer
Please have a look at the following RG links and PDF attachment.
  • asked a question related to Rosa
Question
1 answer
Has anyone tried targeting ROSA locus with CRISPR/Cas9 in L929 cells? If so, does anyone have a wild-type gDNA sequence (or at least a primer sequence and its approx position for PCR screen)?
Relevant answer
Answer
Hello, see attached info may be useful for you.
Good luck!
  • asked a question related to Rosa
Question
6 answers
Beside the scale described by Kathrin Rosing in Leadership Quaterly 2011 which scale do you recommend to measure ambidextrous leadership ?
Thanks for you help.
Relevant answer
Answer
An interesting question and very useful answers given by Dr Jacob [by providing the paper (Innovation capacity building)] ; and Benjamin guidance (Kaupppila & Tempelaar 2016) . Thank you .
  • asked a question related to Rosa
Question
1 answer
I am studying some plant promoter activity. For this purpose I used commercial plasmid to which I inserted examined promoter sequence. The sequence was inserted above two fused marker genes: Gus and Egfp. Wounded leaves was transformed by Agrobacterium tumefaciens carrying the construct. Callus was induced, selected by antibiotic and then shoots was regenerated. I found no expression of examined genes (Gus and Egfp) and very high expression of housekeeping gene (actine). Ct for actin was around 11 cycle. At the level of genomic DNA actine rose at 11 cycle but Gus and Egfp at 32 cycle. Why I obtain such poor results for Gus and Egfp? Is it the case of poor transformation efficacy or maybe epigenetic regulation of inroduced promoter and marker genes? What is your experience? What would you suggest?
Relevant answer
Answer
I would use normal PCR to check for the presence of your insert in your potentially transformed plants. It's very common to have epigenetic silencing of transgenes (as you mentioned), but the integrated DNA will always show up with normal PCR. Just remember to include a full set of controls. Good luck!
  • asked a question related to Rosa
Question
7 answers
Rose hexane extraction in lab scale
Relevant answer
Answer
Check out this.
In rotaru evaporator u use the boiling temperature of the solvent
  • asked a question related to Rosa
Question
3 answers
For my thesis project i am doing research on sexual consent specifically within the Dutch context and different generations of men who have experiences with heterosexual sex. In my focus groups i would like to apply the items of the Sexual Consent Scale-(R) (SCS-R; Humphreys, 2004; Humphreys & Brousseau, in press; Humphreys & Herold, 2007), in an intergenerational dialogue on the meaning, definition and practice of consent. I am wondering if people have experience with using the SCS(-R) in focus groups and what are your experiences. Kindly, Rosa
Relevant answer
Answer
ps. I'll use some the SCS-R as inspiration but will not implement the questionnaire
  • asked a question related to Rosa
Question
6 answers
I am reviewing the type of one fungi. It was collected in Italy and in the original description the author said it is growing on Pistacia therebinthus, Rosa and Rubus. But the type is constituted by only one branch. So I am trying to figure out what is the plant-host for this fungus.
I did some transverse section and I have some macropictures of the branch (inside the branch is hollow). Are there somebody that can help me? I do not know how to identify wood using anatomy, but maybe somebody can figure out at least at family level. It would be good enough to distinguish between Anacardiaceae and Rosaceae for the host of this fungus.
Relevant answer
Answer
Thanks for your answer A. V. Stepanova
  • asked a question related to Rosa
Question
1 answer
Dear Malachi,
when will you release the special issue? And what are you looking for exactly? I am working on a thesis project at the University of Amsterdam department Sociology of Gender and Sexuality. My project focuses on sexual consent among different generations of men and their heterosexual experiences. I will finish the project around july. So there is nothing finished, nor ready. For sure i am looking forward to the issue! Best Rosa
Relevant answer
Answer
Yes Malachi, Please respond to Rosa's question.
  • asked a question related to Rosa
Question
4 answers
I would like to know if it is better to extract the rose essential oil using fresh wet rose petals by using steam distillation or is it more beneficial to use dried rose petals (which is off course more expensive) and use other extraction methods like SFE (super-critical fluid extraction)?
Relevant answer
Answer
primarily fresh leaves are used and moreover the the injection of steam is routine procedure to distill. The disadvantage is that the petal forms the large compact mass where sometimes steam cannot penetrate properly leads to less recovery of oil. As the distillation is refined and the process called cohabitation is used to recover the maximum oil.
As far as SFE is concerned its good method to recover maximum but the disadvantage is not economically viable for growers. Fallowing links may give more insight into the subject.
Regards
  • asked a question related to Rosa
Question
3 answers
I work with Rosa hybrida tissues, and total RNA extraction from it is so hard. There are other plant species which gives good quantity and intact total RNA. So, what is the reason behind the Roses tissues that influence RNA extraction? And how can i customize the conventional protocols to ensure good quantity of RNA from Roses?
Relevant answer
Answer
i extracted total RNA and somehow i got good bands. Then I used DNAse1 to remove gDNA, it failed completely, erasing the RNA bands while gDNA was still there. Then I used reverse transcription to make cDNA. I checked it with UBI, there was no cDNA at all. Don't know whats the issue.
One thing that irritates me is the color of RNA pellet, that is still prominent in the diluted RNA, and cDNA as well. May be the problem is that RNA has some impurities, or it isn't washed completely. What do you experts say?
  • asked a question related to Rosa
Question
2 answers
I am trying to breed two types of Rosa26 knock-in mice together, but I have not had a single litter from 2 breeding pairs over 3 months. The breeders are now 6 months old. One breeder is a tdTomato reporter mouse (Ai14): Rosa26tdTomato/tdTomato. The other is the Ai87 mouse: Rosa26iGluSnFr/iGluSnFr. Technically, I believe the whole insert into the Rosa26 locus is Rosa26 - CAGpromoter - LoxP/stop/LoxP - then the gene sequence (tdTomato or iGluSnFr). I naively thought the offspring would all be Rosa26tdTomato/iGluSnFr and the alleles would segregate that way. Anyone have some molecular genetics insights? or could this just be bad luck?
Relevant answer
Answer
If both breeders are pure line (or homozygous for the alleles), you should have the genotype you want in the offspring. How many litters have you analyzed? How did you test the co-exist of the two alleles in the offspring, PCR?
  • asked a question related to Rosa
Question
3 answers
Hello,
I have a tub garden with four miniature rose plants; among them two with pink colour, one with red colour and another is white. Since the buying of this white plant, it was pure white, but today i have noticed that around 60% of a petal turned into pink, rest all are pure white, even another flower of this plant also pure white. Can anybody help me to understand the reason behind this transformation please?
Relevant answer
Answer
Its due to pigment transformation due to light and dark phase
  • asked a question related to Rosa
Question
5 answers
Hi, I am combining numbers with text, and I was wondering if anyone has done a ranked data analysis with Kendall's tau-b, and written an article about it (or anyone knows such an article). I asked my respondents to rate certain elements and then let them tell why they rated them like that. These are a list of 22 elements, and I only have the five most important factors (and sometimes the five least ones), so i need to do a correlation coefficient between groups and also between elements. If anything like this rings a bell, please send me some links!
Thanks,
Rosa
Relevant answer
Answer
Thanks a lot! Siniša Srečec
  • asked a question related to Rosa
Question
1 answer
Looking for the following:
Rose, E. 2010. The promise of preschool: from Head Start to universal prekindergarten
thanks,
r
Relevant answer
Answer
  • asked a question related to Rosa
Question
7 answers
Dear all, I am currently testing MOS capacitors built on a p-type Silicon substrate (1-10ohm.cm), with a gate oxide made of 3nm SiO2/3nm HfO2. The metal deposited on top of it is Pt (200nm) with an adhesion layer of tianium (10nm). The contact with the bulk is done on the back side of the chips through an Aluminum layer.
I am plotting the CV curve of the devices using a LCR meter 4284A from Agilent. I have been able to extract the Cox in accumulation, which is close to what I expected, but after dropping when increasing the voltage, the capacitance never rose again to Cox in inversion even at low frequency (100Hz). Yet, I have observed strange behaviors that you can see in my attached file, with the capacitance dropping below 0, rising over the expected Cox, and dropping to 0 completely afterwards. This happens only below 100kHz and I think it comes from the large gate leakage wrongly interpreted by the instrument.
How do you think I could solve the issue ? I was thinking about decreasing the temperature during measurement to see if it can solve the negative capacitance effect.
Relevant answer
Answer
In terms of EOT/CET, 3 nm of HfO2 would contribute ~0.8 nm (for k~16), which is not that far from the deviation that you mention observing.
I'd compare these samples to a sample with an identical SiO2 layer without the HfO2 layer. Calculating the difference and using a series capacitance formula would give the most accurate results. We did this for Ta2O5 a while ago, c.f. Fig. 2b and discussion therein https://oxides.net.technion.ac.il/files/2016/05/2013_JAP__Al2O3-Ta2O5.pdf
I also recommend using an optical microscope and measuring the actual gate dimensions, they can vary by quite a bit sometimes.
  • asked a question related to Rosa
Question
6 answers
I wanted to screen genotyping of Gt(Rosa)26sor(CAG) mice but Jaxon lab provide us the genotyping method using qPCR. Is there any normal PCR method that I can use for genotyping for these mice? What primers I can use for this and what will be the expected band for Het, Homozygous and wild-type mice?
Relevant answer
Answer
It worked thanks !
  • asked a question related to Rosa
Question
19 answers
I have tried so many times to extract tRNA from Rose leaves and buds by pBiozol method. My intention is to have tRNA for experiments about miRNAs (as pBiozol can give tRNA with high concentration of miRNAs). Here is the customized protocol that i follow (the steps indicated by * are added by my lab's senior members):
1) crush tissues in liquid N2
2) add pBiozol: 0.5ml for 0.1g tissue, 1ml for 0.2g, 5ml for 1g, and so on.
3) incubate on ice (horizontal placed tubes) for 5min
4) spin @12k g for 2min at 4oC
5) transfer supernatant to a new tube; add 0.1ml NaCl (5M) and 0.3ml chloroform (if 0.1g tissue used in step 2)
6) spin @12k g for 10min at 4oC
7*) transfer the supernatant (uppermost phase) to a new tube; add the same amount (as of supernatant) of phenol:chloroform at 25:24
8*) spin @12k g for 10min at 4oC
9*) transfer the supernatant (uppermost phase) to a new tube; add the same amount (as of supernatant) of chloroform
10*) spin @12k g for 10min at 4oC
11*) transfer the supernatant (uppermost phase) to a new (final) tube; add 1ml of isopropanol alcohol and incubate at -20oC for 2 hrs
12*) spin @12k g for 30min at 4oC
13*) discard supernatant, add 80% ethanol
14) spin @12k g for 10min at 4oC
15*) repeat step 13 and 14
16) remove all the ethanol, air dry pellet for a few minutes, add DEPC water 20-30ul, store at -80oC
17) check quality and quantity by nanodrop and gel electrophoresis
(all the chemicals used are pre-chilled; tubes are worked on ice; extra care for avoiding contamination).
I use young leaves or flower buds. I just assume the weight of tissue as 0.1 or 0.2g (may be i am wrong, but how can i weight the tissue before adding the pBiozol, it seems impossible to me)
now the question is, where is the problem? i mostly get no bands, smeared band, or dim 28s band..
Please help me, i have tried more than 30 times
Relevant answer
Answer
Hi Muhammad,
1) To answer your question: The easiest way to measure your tissue's weight is the following:
1. Put a fresh, empty, closed tube with sample ID label onto the balance, than tare it.
2. Remove the tube from the balance. Put the tube in cold evironment
(preferably onto dry ice).
3. Do the snap-freeze process of the plant tissue with LN2 then transfer the frozen sample into the cooled tube.
4. Put the tube with sample in it onto the tared balance. Than it measure the sample's weight. Put back your tubes onto dry ice until you can begin lysis buffer addition.
5. Repeat this process with all of the samples.
2)
I think the problem is lenght of the time between the sample snap-freezing with LN2 then lysis buffer addition. Try to reduce to minimal time between these steps. RNases are really stable and high activity enzymes, so they need only litlle time to disrupt RNA.
Or might can be problems with the efficiency of the lysis. The plant cell's wall can be hard to disrupt. You can try mechanical methods (steel beads or steel blade disruptors to homogenize your frozen plant tissues with your lysis buffer (pBiazol).
I have got many years of experience in RNA isolation. Please let me to note some suggestions to you.
The Trizol or any similar acid-guanidin-isothyocianate-phenol based lysis buffer effectively inhibit RNases in your samples. The main point is to add the Trizol immediatelly to your sample after thawing followed homogenization by pipetting the cell pellets up and down for severeal times. Then use the classical phenol-chloroform-isopropanol purifing method. I've read that pBiozol is more than GITC/acidic phenol based lysis buffer (beside the earlier compounds it consists ammonium-tiocyanate and 2-mercaptoethanol also - later can irreversibly denature RNases by reducing disulfide bonds and destroying the native conformation required for RNase functionality.
Alternatively you can use the Trizol lysate directly in Direct-zol RNA spin column kit https://www.zymoresearch.com/media/amasty/amfile/attach/_R2060_R2061_R2062_R2063_Direct-zol_RNA_MicroPrep_ver._1.0.0.pdf). I have very good experiences with this kit in human chordoma tissue RNA isolation in the terms of RNA quality and quantity. I have used the Trizole lysate after chloroform phase-separation step in the above kit. To decrease the protein contamination in the further purification steps
GITC/acidic phenol lysis combined with silica-based column methods
(Pros: after phase separation step no need to use chemical hood, can be high throughput Cons: more expensive than GITC-based methods
GITC/acidic phenol methods
(Pros: the cheapest, intact total RNA (including rRNAs, miRNAs) can isolated with high yield, Cons: toxic organic solvents (phenol, chlorophorm) (need a chemical hood), very problematic to use in high throughput).
Bests,
Tamas
  • asked a question related to Rosa
Question
1 answer
I am looking to use ROSA mTmG mice with a cell-specific Cre. I will have tissues fixed in 4% PFA, paraffin-embedded, and sectioned for studies. Is the signal from tdTomato and GFP strong enough that I don't need to come in with an antibody for immunofluorescence staining?
I am interested in staining for other protein targets on these tissues. I'm wondering whether the signal is too strong in the green and red channels from this mouse and if this will make staining other targets difficult, as I wouldn't be able to use the red/green channels as readily. Thanks!
Relevant answer
Answer
Hi interesting question. I recently looked at the cre tissue specificity, using the tdTomato reporter system. I believe there are a number of potentials issues that depend on tissue processing. I was informed that fixation in 4%PFA and paraffin embedding substantially reduced the fluorescent signal, I was advised to use the antibody detection system to detect positivity if I used this system. However I embedded the tissue in OCT for frozen tissue sectioning, this preserved the fluorescent signal. Very strong signal. I would check the intensity of the signal in the cut sections before proceeding with any IHC.
  • asked a question related to Rosa
Question
2 answers
Several cases of Rs in greenhouses were recently reported (First report of bacterial wilt caused by Ralstonia solanacearum in ornamental Rosa sp 10.1094/PDIS-02-16-0250-PDN, etc.). Is there any reports on Rs on tomato in hydroponic culture?
Relevant answer
Answer
Though its not my field , despite Rs being rhizosphere inhabited , its reported under hydroponics...
  • asked a question related to Rosa
Question
6 answers
We have used the mTmG reporter for a few years now and we fix our mouse tissues with Methanol/Acetic acid/water o/n before sucrose cryopreservation and cryo-cutting. Suddenly, we see no red or green whatsoever. SOmething has gone awry technically as JAX tells me they have heard no complaints of silencing of the ROSA locus where the reporter is knocked in. Has anyone suddenly lost the ability to see fluorescence staining in the mTmG mouse?
Frustrated,
Joe
Relevant answer
Answer
Yup; twas the fix! Very strange since we have used methanol/acetic acid fix for years w/o any issue. The 4% PFA fixed the problem. I was initially concerned that the locus was silenced but JAX indicated they heard no such feedback from the mouse community.
  • asked a question related to Rosa
Question
11 answers
i culture Rosa shoot tip in MS medium at beginning it started very well but after 2 weeks it tend yellowish and finally necrotic. paper report that increase Iron to 20 percent and calcium 50 percent solve the problem i increase it but there is no good result. Is there anybody can help?
Relevant answer
Answer
You could ask the Debener Lab in Hannover/Germany I think they should know about tissue culture of Rose
Mrs. Winkelmann also has a lot of experience in tissue culture:
  • asked a question related to Rosa
Question
13 answers
Hi everyone,
I am writing a research proposal and wondering which approach to use. Currently working at a university that has implemented a gender-based affirmative action program. I want to find out if the perceived barriers and facilitators of women have changed while participating in the program. I think perhaps a case study approach would be approrpiate since I have a specific program, but I am also trying to get to know their experiences. Any help would be much appreciated.
Kind regards,
Rosa
Relevant answer
Answer
Actually, Brendan recommended that book, while I personally think it can do as much harm as good. In particular, it leads students to think that they have to choose one of his five traditions, while I prefer a more flexible way of doing things that is not based on a limited number of "approaches."
Which raises the question of why you need a label like "case study" or "phenomenology." If this is something that your advisors are demanding, then that makes it important. But if it is just something that is bothering you, I would recommend that you think more about how to collect and analyze your data, because that is what will produce your results.
  • asked a question related to Rosa
Question
18 answers
I filtered 15 genes from Rosa hybrida degradome. I tried every possible field in primer3,5, ncbi, etc. May be there is problem in specifity. NCBI has no data for Roses, but we often match with the very similar genome Fragaria vesca. However, the specificity is mostly different from the Rose transcriptome database developed by Uni of Cornell. 
Relevant answer
Answer
Dear Muhammed,
I always use the '2+4 rule' for my primer design.
The '2+4 rule' is a rule of thumb or empirical formula, but it works very well in the practice.
The other formulas are theoretical approaches.
Try to use standard primer (10 A/T and 10 G/C). 
G/C rich primer are often unspecific.
Best regards,
Thomas
  • asked a question related to Rosa
Question
8 answers
Hello, 
Can anyone direct me where I could source free high resolution (30m or less) satellite maps that I can use in ARCGIS to do habitat classification? The study area is 100 sq located in the district of Moyamba in Sierra Leone. 
Thanks
Rosa
Relevant answer
Answer
Sentinel-2 has resolutions of up to 10 meters (depends on the bands, please see link 1) and is operational at a high repeat cycle.
Sentinel-2 images date back to 2014 and can be searched and downloaded after free registration the follwing services:
If you need data before 2014, you can search within the Landsat collection which dates back to the 1980s. They have resolutions of 30m and a huge image archive:
  • asked a question related to Rosa
Question
6 answers
The phrase 'mulata toribia' refers specifically to Encarnación Ezcurra de Rosas, the wife of the notorious dictator, Juan Manuel de Rosas. It was used as an insult by her enemies, the Unitarians. 'Mulata' refers to her dark hair and skin but 'toribia' is not as easy to define. I have looked at some explanations suggesting that it means 'controlling', 'domineering', 'spiritually aware', 'intelligent' and 'determined', but I am unsure as to whether any of these translations are accurate. Any further advice on this matter would be much appreciated. Thank you. 
Relevant answer
Answer
Is the 't' in toribia in lower case? As I am sure you know, Toribia is a proper name. There is even a beate of that name. Although I do not know if the reference belongs to a certain person, I venture to suggest as a starting point for your research that such names were mostly given to people of the lower social classes (as it still happens today), so that it must have been somewhat of a pleonasm or generic term to call someone a mulata Toribia, something akin to calling someone an "india María"
  • asked a question related to Rosa
Question
4 answers
if  Xylella fastidiosa transmitted by Edwardsiana rosae,  between what host.
Relevant answer
Answer
Dear  Masoud Latifian
Thank you, during my search no one mention the exact spp., I found Edwardsiana rosae on a host have phytoplasma-like symptoms for of that I need real connection between them .
Regards
Houda
  • asked a question related to Rosa
Question
6 answers
Dear Colleagues, I am preparing a paper about Chlorophyll a and Chlorophyll b in the rosa species. Can anybody help me with the in the Different chlorophyll formulas ?
in the Different chlorophyll formulas The amount of Chlorophyll a and chlorophyll b in the rosa species are different. How should I understand this difference?
Relevant answer
Answer
Maybe the following papers would be interesting to you:
Porra, R., et al. (1989). "Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy." Biochimica et Biophysica Acta (BBA) - Bioenergetics 975(3): 384-394.
Lichtenthaler, H. K. and A. R. Wellburn (1985). "Determination of total carotenoids and chlorophylls a and b of leaf in different solvents." Biochemical Society Transactions 11: 591-592.
  • asked a question related to Rosa
Question
4 answers
We are trying to improve germination success for rare species/varieties of Rosa and Prunus. Any good scarification/germination protocol information appreciated.
Relevant answer
Answer
For the germination  of the  difficult  Prunus and  Rosacea seeds   are  useful   H2SO4 solution, however  with very careful care:  
1. Only 20 %-30 % of  acid solution should be used
 2. Exposition  not  more  than  20 -30  minutes,  for some species (Cornus mas)  even 40  minutes
3. After removing of seeds  from acid rinse  with 10 %  sodium bicarbonates solution
4. Washing in water 
5. Drying out  
6.  Keeping in moist condition  3- 5  C  before of seeding..
  • asked a question related to Rosa
Question
10 answers
I want evaluate species of Rosa relatively of ecological factors within my study area. But I found that ecological scales of Ellenberg, Landolt, Tsyganov contain no many species of this genus. Are there sources where I could found these or similar data?
Relevant answer
Answer
Hi,
You can also see: Zarzycki K., Trzcińska-Tacik H., Różański W., Szeląg Z., Wołek J., Korzeniak U. 2002. Ecological indicator values of vascular plants of Poland. Ekologiczne liczby wskaźnikowe roślin naczyniowych Polski. W. Szafer Inst. PAS, Kraków.
  • asked a question related to Rosa
Question
15 answers
It was collected from Eastern Anatolia, Turkey
Relevant answer
Answer
Rosa foetida Herrmann has yellow petals.
Anna
  • asked a question related to Rosa
Question
3 answers
Explants show chlorosis after 2 week in medium.
Relevant answer