Questions related to Rooting
I think to improve the growth of pomegranate seedlings when they planted in the orchard soil because some of seedlings may be died or growth weakly. The rooting stimulants were applied to get out more roots of cuttings, therefore I hope the ability of these stimulants benefit the transplants when were planting.
Anther derived plants of oil seed(niger) were raised in-vitro. After successful rooting, hardening is becoming difficult . Can any one suggest for proper hardening conditions for 100% plant survival.
I have done genetic transformation of tobacco with leaf disc method. Now, my calluses have been sprouting small leaves. I want to confirm the presence of my transformed gene. Kindly suggest me, how long should I wait to shift them to shooting and rooting media? Or can I confirm the presence of gene at this stage without utilizing whole callus? Also, they have been catching fungus, so I am left with only few calluses. Shall I rely on them? or Shall I infect more explants?
I would be interested to hear about rooting depth of alder trees and the effect on peat quality. To my knowledge the peat layer of alder swamps is comparatively shallow compared to other fens systems, i.e. in some case only few decimeters. I would suspect that this phenomenon is inter alia also linked to the fact that there is oxygen release in the rhizosphere but also the N fixation by roots might be of importance?! For the rooting depth I found this work: https://academic.oup.com/forestry/article/83/2/163/519324 stating that it can be up to 5 m?! However, I wonder if peat of alder swamps is typically more decomposed because of the mentioned root activity or is it the fact that naturally alder swamps experience naturally larger water table fluctuations?!
Hi to everyone,
I´m transforming explants of Nicotiana tabacum with agrobacterium following Clemente´s procedure. In what size or stage of the shoots is advisable to transfer them into rooting medium? And how much time it takes to generate roots?
Thank you in advance.
Hello everyone! I am looking for some statistical help!
I am working in in vitro micropropagation and one of the aspects that I am studying is the rooting of plants in 10 different growing media. For that, I categorize the rooting of the plants as 0 (no roots) or 1 (rooting). Therefore, I have one categorical independent variable (rooting medium) with 10 different categories and one categorical dependent variable with two categories (0 and 1). My sample size is 30 plants per medium.
From those numbers, I obtain a rooting percentage for each medium and I would like to now if this parameter differs significantly between the different media.
I would like to know how I should compare my data. These are the tests I have done but do not know which one to use:
- 2x10 contingency table in SPSS and Chi-2 test. This gives me a p value for the whole experiment. For post hoc, I have used a Z-test.
- I have also tried a univariate GLM in SPSS with a tukey post hoc test.
I do not know if both are valid or if I should just stick to the contingency table.
Any help would be appreciated
These cannabis plants were healthy and green for 8 weeks and they started browning when I put them in rooting media. No rooting achieved and they are turning brown.
I want to study vegatative propagation by cutting. My plant is parrotia persica.
Did you study on this plant's propagation?
Please say your experience to me.
I have a problem with magnolia shoots rooting. For the rooting of in vitro produced shoots, I have used MS basic medium containing auxins (NAA and IBA, ranging from 0.5 to 4.0 mg l−1), but withour effect. I also tied ex vitro roooting method. The in vitro produced shoots were treated with comercial rooting auxin (IBA). The bases of shoots were treated with IBA for 12 h. Auxin treated shoots were directly transferred to soil and kept in the greenhouse for rooting. It was done 2 weeks ago I steel wait for results. Does anyone experience with magnolia in vitro propagation? Thank you for any suggestion.
I have been trying to generate transgenic poplar plants NM6 in WPM media and with different rooting hormones. My plants haven't been rooting so far.I transferred some into 1/2 MS with IBA, but there are still no roots. I welcome any suggestions you might have!
I have a subset of eukaryotic proteins for which I want to find out from which prokaryotic phylum these proteins originated from. So I blasted these group of proteins against the prokaryotic database and have a set of hits. I grouped the eukaryotic and prokaryotic proteins into protein families using ML and made phylogenetic trees for each family using RaxML. My question is which outgroups can I use to root the phylogenetic trees and whether is it even necessary beyond what is done by default by RaxML? Is drawing conclusions about sister group relationship between eukaryotic and prokaryotic proteins dependent on rooting the trees with proper outgroups?
I am currently working on the adventitious rooting through cuttings, I have used WinRHIZO 2013 software to measure root parameters including root volume. I am confused about the increase or decrease in root volume. Does anyone know that what reasons may cause increase or decrease in plant root volume?
Hi everyone we are in the process of standardizing the micro-propagation protocol of culinary banana (ABB) for commercial scale production. But, unlike Grand Naine and other table varieties of banana these culinary types are showing more elongation, lanky and week growth while hardening (Picture attached). Further we also recorded low survival in field condition. In this contest i am planning to use some additional media supplements in in vitro (may be before in vitro rooting step) to improve the plant health condition there by more field survival. I am requesting the experts to suggest the media supplements to produce the good quality planting material.
I have transformed nucleus of Nicotiana benthamiana with LBA4404 Agrobacterium strain harboring gene of interest that was Chloroplast Transit Peptide + aadA gene for spectinomycin resistance. I infected leaf discs with LBA4404 and grown them on Spectinomycin containing MS medium, spectinomycin seems to be working properly as the color of leaf discs has been bleached out and the callus formed is also swollen, it is almost 3 months and my callus didn't started shooting and rooting yet. I have used the appropriate Hormones for that purpose which are: BAP 500 uL/500 ml, NAA 50 uL/500 ml, Spectinomycin 750 uL/500 ml, Cefotaxime 500 uL/500 ml. Why am I not getting shoots and roots in my calli, is there any problem with the Hormonal or Spectinomycin concentrations that I am using? Kindly suggest me the best way to counter this problem. Reference papers would also be best in this regard.
I have mutant of kalanchoe I'm currently working with but the young plants are finding difficult to produce enough roots to survive in the soil.
How will I make these plants root?
Any helpful suggestion will be appreciated.
I am studying the effect of sugars on rooting of Dianthus sp. in tissue culture for my internship report and I grew some plants on medium that did not contain any sucrose. The medium did contain 2.46 g/L McCown Woody Plant Medium, 0,1 mg/L IBA and 6 g/L micro agar. Strangely the plants grew just as good as the media that did contain sucrose, and after five weeks the are still growing and of good quality. Could it be that the plants are using the micro agar as a carbon source?
I am using the same medium and PH in which I started the plantlets but I change the hormones to only IBA and my green shoots turned red with
no roots at all.
I work at Center for Advanced research in Plant Tissue Culture, Anand Agricultural University, Anand, India. Currently I am woking on micropropagation of Sandalwood. I have successfully completed multiplication but finding difficulties in rooting phase. I have experimented various approaches for in vitro rooting such as higher auxin levels, combinations of auxins, pulse treatments, various hosts, biotic as well as abiotic stress but none of them have proved viable. The frequency of getting roots is very low (only 1 at every 100). Please suggest a reliable method for high frequency rooting.
I have a problem in rooting of kiwifruit plants in tissue culture condition, I am applying different treatments can anyone help me?
I have been propagating a poplar hybrid (717) in sterile tissue culture with 1/2 MS media and no hormones. My last few batches haven't been rooting well. I have replanted some into 1/2 MS with IBA, but there are still no roots. I welcome any suggestions you might have!
Dear lovely friends,
I am working with many perennials. One of them is Ophiopogon. Maybe sombody know which auxin and at what concentration I have to use for quicly rooting of shoots in vitro?
Each info will be great 😉
I was looking for any reading material about nexus between species successional status and rooting pattern for Australian wet tropical rainforest species? Any help is highly appreciated. Thanks...
I am unable to regenerate plants from the callus obtained from apical meristems. Also, the callus response is not defined and changes frequently and is not replicating. The variety produces a lot of phenolics and before they reach regeneration , they die. I find it hard to decide whether to go for calli that are compact and hard or calli that are friable and wet. The wet ones do not survive on the regenertion (shoot) medium whereas compact ones produce rooting response on the regeneration (shoot) media. Phenolics are a big problem and the type of callus also. Please suggest solutions for troubleshooting the media composition.
I wanna know that, in what way PPO affect rooting potential? in other words, is it probable that agents that decrease PPO activity, result in enhanced rooting?
If so then what is the reason or cause behind it. Is there any paper or article mentioning about it. Please send some paper releated to it.
Many papers are misleading for shoot multiplication, so if anyone have really tried of rooting kindly provide details,,,
I would also like to know which auxin gives the best result with the given species?
It would be a great help,,
Thank you all..
I tried callus initiation in MS media containing different concentrations of BAP, Kinetin and 2,4-D in combinations. The same combinations help to initiate and maintain calli from other explants well but not in case of leaves. In leaf explants,however, I got more of rooting than callus formation with these combinations. Can someone help me with this?
I am getting in vitro roots in my plant of family convolvulaceae on GA3. So can anyone please provide me such papers or suggestions which support my result?
During direct regeneration of Chickpea, the shoot tips start to get brown just after a few day of sub-culturing to multiple shooting medium or to rooting medium and stop responding. What may be the reason?
What is the solution to this problem?
I have a dataset or co-occuring trees sampled from a stand. Predawn water potential varies during the dry season. Since we assume that predawn water potential depends is measured at low (or 0) transpiration rates I tend to argue that the differences are due to differences in rooting depths. However, I wonder have connections of predawn water potential and rooting depths been explored in a systematic way?