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We're working on the description of turtle shell fragments from the late Miocene of Ukraine. There are some unusual traces (shallow parallel grooves) on the dorsal surface of one of the specimen. We tentatively interpreted these traces as rodent tooth marks. Could someone suggest any publications where such traces on either fossil of extant turtle shell are described/figured or at least mentioned. Thank you very much in advance!
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Dear Dr. Kovalchuk,
You are welcome! It's always a pleasure to share information, especially in an interesting topic such as bioerosion in turtle shells, that is generating some very interesting research lately.
Best regards,
Joaquin Pedro
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How can we identify and differentiate upper or lower rodent incisors through only an Incisor SEM photograph?
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I am not an expert in rodent incisors but a key feature that applies to both human and most mammal incisors:
Incisal wear, for the upper teeth the buccal surface is longer and for the lower the lingual surface is longer.
Hope it helps.
Image from Fondriest et al 2012
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I am trying to standardise photos for my research project. I have over 60 photos of rodents that despite being taken on the same camera, same room and from the same distance have variations in light etc.
I am trying to look at redness and inflammation so I want images standardised as much as possible so that blinded researchers can compare them.
is there any software that can standardise the color/lighting and make images ideal for comparison?
Many thanks
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There are two ways to organize your images: either place them in your text next to the paragraph where you discuss them (Figure 1), or put them all together at the end of the essay (Figure 2). Images always need captions. Captions should do two things; label the image and tell us the image's source.( taken from Google 1ly) for research paper purpose
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We are looking for a facility to execute voluntary wheel running exercise experiment in the context neuronal fatigue. Please let me know the any available labs.
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我想我可以帮你。你能留下一个联系电话吗?
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The Promega HiBiT monoclonal antibody (Clone 30E5) works very well for western blotting and immunofluorescence. I wonder if anyone has confirmed that it works well for immunohistochemistry of rodent tissues. Low background staining is critical.
Thank you.
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Hi Michael, I think it is working with immunostaining stain and western blot as well please check this link of the Promega
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If you use/have used a tattooing system for neonate rodents, which one is/was it? And do you like it? We are researching systems for a future project.
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we did not use individual tagging for newborn rodents
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Hello everyone,
I'm planning a study that involves treating mice with the PPAR gamma agonist rosiglitazone. It seems like I have a few options for administering Rosi, including gavage, ip injection, or as a dietary supplement. I couldn't seem to find any literature describing how to impregnate the chow with rosiglitazone. I apologize if this is an ignorant question, but I've never done it before and don't know whether I need a solvent or if I just.. sprinkle it on there. Furthermore, I'm not quite sure of the advantage to adding bioactives to chow compared to ip injection. Obviously with ip you ensure dosage, but in your experience does diet supplementation still work effectively without the need to handle all the animals daily? Thanks for your time!
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I did an experiment years ago administering rosiglitazone in rodent chow. By that time, I had experience with drugs delivered in the water and orogastric gavage, and I wanted to try another route because of some limitations I had encountered in the former routes.
First, I searched for the dose (mg/kg BW). Then, I estimated the average food intake (g/day) to know how much drug I should add per g of diet. Finally, I bought the drug and send to the company that prepares my purified diets.
It worked nice for rosiglitazone and the other 2 drugs that I used, but it has some limitations.
The first issue is that food intake varies, and on each day, mice will ingest a different amount of food and thus a different medication dose. In addition, if the animal gain or loose weight and does not change food intake, drug doses also change.
Here are the papers with rosiglitazone:
In this paper we discuss routes for drug administration:
Hope this helps!
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It seems to be transmitted by rodents. As we know, sewer mains are habitat for rats which frequent cities. Thus, there is a question of transmission prior to the raw sewage reaching the wastewater treatment plant. Thus, there is a question of the pox virus surviving the treatment process. It has been proposed that SARS-CoV-2 can be picked up by fecal bacteria as lysogens, is the Monkeypox able to also do this? If rats (rodents) can carry this, is there any chance of also carrying both Monkeypox and SARS-CoV-2 and a reassortment?
Dr Edo McGowan
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The most common cause of a foreign body ingestion is when a person unintentionally or unknowingly swallows an object which is either too large, sharp or toxic to pass through the digestive tract without causing potential harm
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I'm interested in estimating how much time do birds (independent of their taxonomic group) spend in contact with soil, particularly on heathland ecosystems. I would use this information as a proxy to assess whether birds would be more or less likely to pick up (either accidental or active -phoresy-) non-parasitic soil arthropods in comparison to other groups (e.g. rodents, herpetofauna, etc.). This approach is at an exploratory stage, so depending on how detailed is this information -if available-, I will decide if it makes sense to use it or not.
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If you are basing your estimation of the time the birds in question are spending in contact with soil on observations using a unit of time that fits your needs/options for observation seems reasonable. Take your whole observation time and subdivide into intervals of equal length documenting all behaviors relevant for your research topic for each interval. You should define different behaviors if you want to document them before the observation (for example by an initial observation phase used to catalogue behaviors).
My (limited) experience is that e.g. in 30 second intervals multiple types of behavior can occur (for example a duck can walk to the edge of pond climb down a little incline to get into the water and start to swim), so very long intervals might not result in accurate documentation of behaviors. But much of this depends on your set up (are you just interested in being in contact with soil or not, or do you want to be able to distinguish between different interactions with the soil, like scratch digging, walking, feeding on soil arthropods etc.; how long will observation be etc.).
Alternatively instead of doing observations yourself you can try to check if there is sufficient literature on the behavior of relevant bird species (documenting time budgets with behaviors useful to address your questions) in the type of ecosystem you are interested in.
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Hi there,
I would like to learn more about the species, which are a reservoir for Monkeypox.
Has anybody got a comprehensive list of species, which can catch and retransmit Monkeypox? Cherish your wisdom.
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Iiterature IS scarce about this
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Corticosterone is a component of HPA axis, also known as stress axis of body, therefore, it is one of potent marker of anxiety and depression. I suggest you to study the corticosterone along with the CRF in hypothalamus and dopamine level in brain. You can also refer my recent publish paper (1) Mishra A, Singh KP. Neurotensin agonist PD 149163 modulates the hypothalamus-pituitary-adrenal axis impairment in lipopolysaccharide-challenged mice. (2) Ankit Mishra and K.P. Singh (2022): Neurotensin agonist PD 149163 modulates the neuroinflammation induced by bacterial endotoxin lipopolysaccharide in mice model.
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Plant faces various problems on their survival process. It can be abiotic ( Unfavorable Temperature, Humidity, or any climatic conditions) and biotic (insect, pathogen, weeds, rodents or any other living factors). How can you differentiate these problems based on physical appearance on the field?
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Certain clues such as insect damage can be easy to diagnose while other things such as a slight color change can indicate a deficiency of a particular nutrient. Abiotic stress often impacts more than one species at a given site (e.g. drought, or too much exposure to water inundation). It really comes down to knowing the plant species and the ecosystem that it occurs in.
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Dear researchers, according to your practice in field of staining bone marrow cells in rodents.
What are the types of staining and which is the best and easy used differentiating BM cytology.
Dose anyone have atlas or reference guide for rats bone marrow cytology?
Thanks in advance
Dr. Ali Alchalabi
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If we want to detect infectious agents, there are various histological stains for the agent. however, if we want to look for different cells, we can use immunohistochemical stains. Which different cells do you want to detect?
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Hi everyone,
I have recently ventured in using the elevated plus maze test. I am having a hard time finding a paper where the time spent in the neutral area is analyzed. Can someone point me in the right direction? I am wondering what this parameter indicates.
Thank you!
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Thank you Mahour Farzan !
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I need book/literature/research article about skull anatomy of small mammals with special reference to India.
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Hi!
The following paper might be a good starting point to find more identification guides.
"Identification of Shrews and Rodents from Skull Remains according to the Length of a Tooth Row" (Balčiauskienė et al. 2002)
doi: 10.1080/13921657.2002.10512524
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What is the pathogenicity and virulence of following specific pathogens?
  • Rabbit rotavirus and Encephalitozoon cuniculi in rabbits
  • Pneumocystis carinii and Staphylococcus aureus in rodents (rats and mice)
Are there any related references about these?
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Hello dear
See attached link please
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Bone tissue homogenate procedure for performing ELISA.
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Hello Nikita,
thank you for sharing this interesting question with the RG community. As an inorganic chemist I'm absolutely no expert in this field and I'm not in the position to provide a qualified answer. However, I made the experience that it often pays off to search the "Questions" and "Publications" section of RG for related questions and useful literature references. For example, please have a look at the answers given to the following closely related technical question which has been posted earlier on RG:
Can anybody advice a reliable model of tissue homogenizer for cartilage and bone grinding?
This question has received 10 potentially useful answers. Perhaps the easiest way is to grind the bones using a mortar and pestle containing liquid nitrogen and subsequently extract the RNA, DNA or proteins. For the very convenient use of a Bullet Blender centrifuge which can homogenize tissue and bones using bead disruption please have a look at the following interesting article:
This paper is freely available as public full text on RG. Good luck with your research work!
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It has long been recognised that bumble bees most often nest in abandoned mammal (usually rodent) nests, and I believe various researchers have tested the effects of mammal scent in attracting queens to field hives, but I'm not aware of any positive results. However I have not been keeping up with literature for a while and may have missed it.
Do you know of results confirming the effect of mammalian odours?
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Dear Nelson,
this is a very interesting technical question which I personally never thought of. To my knowledge, the reason why bumble bees very often nest in abandoned mammal (mice and voles) nests is that they cannot dig themselves. According to many links available on the general internet it is generally accepted that some bumble bees can faintly smell the mice and voles that once built the nest and follow this scent until they find the entrance. Unfortunately I could not yet access a scientific article in which this behavior has been proven.
Good luck with your work!
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Say a researcher was interested in determining the number of adults vs. juveniles of species X trapped during a small mammal survey. Does there exist a relatively reliable way of doing this based on standard field measurements?
Let’s say a total of 200 individuals of species X were sampled, and the following data recorded: sex, total length, tail length, hind foot length, ear length, and weight. For the sake of this question imagine no additional data is available (e.g. additional observations recorded in the field, access to collected specimen material, etc.).
  1. Is there a way to ascertain a point or “threshold” from a range of data based on the distribution of values to distinguish between juvenile and adult individuals with a meaningful degree of accuracy? For example, male species X with weight > 142 g = adults; < 142 g = juveniles.
  2. If yes, which of these measurements would be most indicative? Or perhaps a combination/ratio of more than one (e.g. ratio of hind foot length to ear height > 1 = adult, etc.)?
Thanks, and looking forward to the feedback.
Evan
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Search :
Crouched Locomotion in Small Mammals: The Effects of Habitat and Aging
by Angela M Horner (2010)
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I am recording local field potential from stratum radiatum at CA1 by stimulating Schaffer Collaterals. I wanted to get a better idea of the size of SR as I do not want to get too close to the pyramidal layer or hit the lacunosum-moleculare layer. Literature search did not return any measurements. Also, how far do dendrites extend from the cell body at CA1 pyramidal layer into the deeper layers of the hippocampus?
Thanks
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Thank you Max Anstötz and Paula Parra .
I will try improving the illumination. The size guidance are helpful. Thanks
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Hello everyone!
In the Fall, I am curating an undergraduate research experience laboratory, and I am going to induce some restraint stress on 150 gr SD rats. The restraint tubes are quite expensive on their own, and I will need about 16... has anyone had any luck with cheaper (even DIY) alternatives?
Thank you all so much!
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Hello Cecilia; I have used "thin-wall polycarbonate tubing" for restraining reptiles, including rattlesnakes. It comes in many diameters in stock lengths that are easy to cut. You might try shopping for some of that. Best regards, Jim Des Lauriers
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Hello,
I am looking for a user friendly python based soft for automatic classification of vigilance states like AW, QW, nREM, and REM in rodent EEG.
If you have this information please advise.
Thank you!
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Hi. I hope the following article could help you:
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Possible and best methods to measure the blood pressure of rats.
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Hi. I hope the following link could help you:
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we would like to know about other methods for modelling osteoporosis in rats/mice without making ovariectomy in efficient and time-wise manner
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Hello,
I would like to know what are the commutator systems that are being used in other labs for in-vivo neural recording (and stimulation) in rodents?
In the lab we tested some cheap non-motorized commutators such as adafruit's and moflon's. The initial mechanical resistance is very high for a mouse to easily make it turn.
I know there are some motorized options but they are super expensive (>4k) and/or super big (need to be installed in the ceiling)
Thank you!
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Hi Sirenia,
My message is probably too late. How is your experience with those swivels?
We use a similar swivel for recording rats. They work well mostly but one of three or four makes some noise during spinning. But in generally we are satisfied. Now fortunately there is a big selection of different swivels in amazon. I guess that a bit more expensive one conducts a a better quality signal.
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How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval before the next sampling the same area?
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Thank you very much Magne Neby Andrew Hawkey for your suggestions I will definitely have a look into them
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Does anyone know of any standardised tests of very long-term memory (weeks to months) in rodents?
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Conditioned taste aversion is a long-lasting, standardized test of single-experience memory. In the paper below, rats without extinction training showed a comparable suppression of tastant consumption 5 and 21 days after the initial experience. Presumably, this should last even longer than that without needing retraining.
Rosas, J. M., & Bouton, M. E. (1996). Spontaneous recovery after extinction of a conditioned taste aversion. Animal Learning & Behavior, 24(3), 341-348.
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I need to isolate fraction of nuclei from rodent brain and liver. Fraction must be free of ER, other membranes, mitochondria etc. (or with trace amount of other cell organelles). Can anyone help? Please don't advice kits
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Hi, I have tried this method for fractionation of my HEK293AAV cells. I am checking fractionation purity with GAPDH and mitochondrial gene distribution in both fractions, and I keep getting more mitochondrial gene detection in the nuclear fraction than in the cytoplasmic fraction.
I have checked under the microscope and I can see that the cells have been lysed and the nuclei remain intact. I have also carried extra washing steps to try to minimize cytoplasmic contamination, but it doesn't seem to solve the problem.
Would you suggest anything to improve this?
Thanks a lot!
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I came across two papers (Shcheglovitov et.al., 2013, Nature; Zaslavsky et.al., 2019, Nat Neuro) where they mixed neurons from two genotypes and seeded them on a bed of rodent astrocyte. How does one use this setup as a readout for any phenotypes?
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Yojet,
A nice advantage of neuronal co-cultures of this type (when one can distinguish between the different cells) is that they allow analysis of the behaviour and morphology of both types of neurons side-by-side while reducing culture and staining variability to a minimum.
In the particular case of these papers, it seems to me that the authors want to analyse Shank2/3 role at the postsynapse while keeping presynaptic terminals as WT. Therefore, a "sparse" co-culture helps analysing morphological features while also provides a source for mutant postsynaptic compartments as well as WT presynaptic buttons to form hybrid synapses. I will here admit that I haven't fully read the papers and hence my view could be an oversimplification. So, this is what I got from an overview of the results and what I can assume from my own experience in co-culture.
Hope this may help you.
Cheers,
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I have been looking at weight values for rodents in the family muridae, specifically subfamilies: gerbillinae and deomyinae. I found some considerable discrepancies in the values for the same species from different references. Generally, I get similar values from sources concerned with African mammals (Mammals of Africa, Kingdon et al, 2013; Mammals of Sub-Saharan Africa, Monadjem et al, 2015; The Complete Book of the Southern African Mammals, Mills and Hes, 1997; The Contemporary Land Mammals of Egypt, Osborn and Helmy, 1982). The values I get from other sources, namely PanTheria, AnAge and Alhajeri et al (2015) are mostly similar amongst themselves but can be very different from those reported in the first (“African”) set of references.
The similarity within set cannot be solely explained as repeated citations from the same old reference; so I was wondering if it can be explained by biogeographic trends within widely distributed species. In other words, the set of references concerned with Africa is reporting species values from African populations only; while the other references report values from the world-wide distribution of the species. The observation that species with African and extra-African populations have wider ranges of values reported in PanTheria, AnAge and al-Hajeri compared to those in “African” sources for the species is consistent with this hypothesis. Furthermore, whenever a species is endemic to Africa, the two sets of references seem to largely agree.
Could somebody please corroborate/debunk this idea of mine, or suggest other explanations for these puzzling discrepancies?
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I would check other values like head-body length to see if there is intrapopulational variation. If the discrepancy is really due to intraspecific variation you would expect linear dimensions to show a similar pattern to body mass, since you would be measuring bigger dimensions on bigger animals.
However, it's also possible that the reported body masses are just plain discrepancies. I've noticed a lot of second-hand databases like PanTheria can be extremely inaccurate when it comes to reporting body mass values because they often just collate them from the previously published literature. A lot of times studies will use ballpark values or the midpoint of guestimated ranges from sources like Walker's Mammals of the World, which aren't very accurate or precise. A good example of this is the South American rodent Dinomys branickii. Most studies using body mass for Dinomys will report a value of 10-15 kg or 13-14 kg, which seems to be drawn from a ballpark range of 10-15 kg mentioned in a paper from the early 20th century (maybe Allen 1942). This doesn't appear to be based on any measured specimens, and no studies list specimens from where these data were collected. However, actual weights of Dinomys based on first-hand measurements of collected wild individuals (e.g., Osbahr et al. 2009, Gottdenker et al. 2001) finds that the body mass of Dinomys is actually only about 9.5 +/- 1.1 kg, a much lower estimate. Larger individuals are known but are highly gravid, captive ones. Nevertheless, people have been reporting a 13-15 kg body mass for Dinomys as if it were gospel.
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I am interested in collecting brain size (endocranial volume) data for several modern species of mouse-sized rodents. However, I am struggling to figure out the best methodological way to obtain this data.
The gold standard for measuring endocranial volume would probably be to take CT scans of the specimens and measure endocranial volume off of the virtual endocasts. However this would be prohibitely expensive as it would be nessary to scan hundreds of skulls to obtain decent sample sizes (N > 8-10) for each species. Sufficient sample sizes exist but getting the data from them is the hard part. Only getting data from one or two individuals per species would not be rigorous enough to produce trustworthy results. Even if I got a grant to do the scanning the specimens I am interested in are housed in distant institutions that I can plan collections visits to but are too far away to visit regularly. I cannot drag a CT scanner to these institutions to get the neceasary data nor take out loans for hundreds of specimens.
The other major way I know that people have measured brain size is by filling up the endocranial cavity with glass beads or lead shot and estimating the volume from the density. However, at smaller and smaller body sizes lead shot or other spherical globules are going to increasingly poorly correlate with brain size, for the simple reason that you can pack fewer granules inside a spherical chamber. At large sizes the relative error is negligable, but at small sizes spherical pellets will poorly model the volume of the cavity and accuracy will become increasingly worse. I've even seen this in the literature with some small rodents, in which reported cranial volumes measured with lead shot seem suspiciously large or small compared to other studies on the same species, with endocranial volumes sometimes differing as much ad 50-70%. Additionally many natural history museum will not let you bring pellets like lead shot into the collections for fear that it could hide pests or get scattered and cause problems (something like this has happened to me a couple of times: I used sand bags to prop up larger modern mammal skulls for photograph and nearly got thrown out of the museum collection for bringing outside sand in).
Given this, what would be the best way to measure brain size in museum collections of mouse-sized rodents?
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Hello Russell: Tungsten carbide is used as an abrasive. It comes in grit sizes that vary from extremely fine powder to pretty coarse grit. If you can get permission from collection managers, a heat sterilized jar of the grit of whatever sizes you determine to be appropriate, non-toxic and pest-free. A graduated cylinder provides the volume directly. Were I a collections manager, I can't imagine what the problem would be.
Apparently specimen loans are out of the question. Hmmm.
Best regards, Jim Des Lauriers.
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We need to analysis social network of multiple individuals. Such like record contacting time between multiple individuals(5-10 mice group) to find out the most popular one (social centrality or spending most time with) in a open field. Besides, we also need to differentiate aggression, random contact, and friendly contact. Some of my friend suggest me to use "DeepLabCut", but I am not sure if anyone has a better suggestion to achieve what I need. I am kind of software-challenged person. So if you guys have a easy user manual or toolkit, please share with me. Thank you!
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You might want to have a look at Doris (Detection of Object and Tracking) at http://www.boris.unito.it/pages/doris.html . It's a free and open-source software, should be a bit more friendly than DeepLabCut, but I haven't tried it yet.
Chiara
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I have read several articles mentioning the preparation of the drug disulfiram (DSF) in olive oil for oral gavage of rodents. However, in those papers, there is no description of how this is achieved. For one of our projects, we plan to use this approach, and we tried to resuspend DSF directly in olive oil at room temperature at a concentration of 12.5mg/ml, but this is not working properly. Heating helps but we may have a risk to alter the drug. Has anybody experience with DSF/Olive oil preparation? Many thanks in advance, Olivier
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By Rotary evaporator
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Please share your/others experiences/findings related to PCOS in rodents.
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The following article is a detailed overview of your query. Kindly read it
Shi D, Vine DF. Animal models of polycystic ovary syndrome: a focused review of rodent models in relationship to clinical phenotypes and cardiometabolic risk. Fertility and sterility. 2012 Jul 1;98(1):185-93.
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I would like to conduct an animal study on rabbits. We study brain and systemic toxicity through immunostaining. Perfusion fixation is therefore needed. I found a protocol for rodents by Cage et al. . Does the same exist for rabbits. Could perfusion fixation be done after euthanasia?
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I decided to switch to glass pipettes from Hamilton syringe for AAV injections into the rodent’s brains. Now I am trying to find the best parameters for pulling the capillaries but cannot make the taper long enough. I need to break the taper in order to get 20-50 micrometers tip, the maximal length of such broken taper I managed to get is about 5.5 mm. Does anyone have experience with producing longer needles?
I am using Sutter P-87 puller and Drummond capillaries (ODxID=1.14x0.56).
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We can help with our newly developed MP-500 micropipette puller. Just let us know the specs you requested and will will help figure them out. See file attached for flyer.
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As part of a Covid project we are sampling urban rodents and performing RNA extractions and serology work. We have too many samples to store at -80 and have opted to perform extractions the same day as dissection to limit an extra freeze-thaw cycle. The samples are currently stored as whole animals at -20. Does anyone know how long viral RNA would be stable at those temperatures? We know these conditions are not ideal, but were working under the assumption that if the cells and viral envelopes are intact then some of the RNA should be relatively stable for a number of months.
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Thanks for the reply Mattia, as the animals are currently un-dissected and are in a high number that volume of RNALater wasn't logistically feasible. The sample input for extractions will be fairly large and the RNA will be converted and fragmented to around 150bp for sequencing. Once we begin extractions we will be looking for ribosomal bands to confirm RNA integrity but in the mean time I'm just hoping it is stable within the animal tissue
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Does anyone know where I can find dodecyl propionate? Dodecyl propionate is a rodent pheromone present in rat pup's preputial gland (original paper by Brouette-Lahlou et al., 1991, J Chem Ecol, paper attached).
It seems that this compound does not exist anywhere for sale so I was curious if I can make it synthesized/made on demand? If someone is familiar with a chemistry website/database where I can order pheromones from, that would be amazing.
Thanks in advance!
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Hi Brian, thanks a lot for the reply!
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For sex determination in nutria embryos I used SRY primers, which give a band for the males, as they should, but the primer I used for control, rps12 (S12) doesn't give a band. Are there any useful primers for nutria?
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We know the importance of kisspeptin in rodent fecundity but it's mechanism in laying hens, yet understand?
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Kisspeptins are produced and used during reproduction in birds i.e. are components of reproductive physiology in birds
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Looking for papers which detail the various modes of social organization in rodent species.
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Hi Patrick,
I don't know of any 'short' reviews of the topic as standalone papers with respect to just rodents, but there are number of important books and papers that cover the social organisation of some of the best studied clades in detail, or will cite literature that does so. Not sure how easy they are to come by though in your case (hopefully there is a good library nearby as not sure many of the chapters are accessible on the internet).
- Wolff and Sherman eds 2007 Rodent Societies
- Bennett and Faulkes 2000 African Mole-Rats: Ecology and Eusociality
- Clutton-Brock 2016 Mammal societies. (you'll have to leaf through to find the relevant literature on rodents)
Other useful papers:
- Armitage 1999 The evolution of sociality in marmots. J Mammal 80: 1-10
- Randall 1994 Convergences and Divergences in Communication and Social-Organization of Desert Rodents. Australian Journal of Zoology. 42(4) 405 - 433
- Ebensperger & Blumstein. 2006. Sociality in New World hystricognath rodents is linked to predators and burrow digging Behavioral Ecology 17: 410–418.
And then there are other useful monographs and treatments of some well-studied species such as mara, capybara, various voles, and of course the naked mole-rat.
You should also be aware of a recent push towards thinking about intraspecific variation in social organisation (see others referenced/cited in/by these two papers):
- Schradin C, Pillay N (2005) Intraspecific variation in the spatial and social organization of the African striped mouse. J Mammal 86:99–107
- Schradin et al. (2018) The evolution of intraspecific variation in social organization. Ethology 124: 527–536.
Hope this is a useful and that others can perhaps expand on this.
Jack
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Hi,
I would like to record and analyze ultrasonic vocalization of rodent (rat and mouse) newborn pups, and I find Wildlife Acoustics Echo Meter Touch 2 bat detector and the Kaleidoscope Lite analyzer software. Do anybody tried this device to record rodent USV?
Thank you in advance!
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I do not have direct experience in that field, but do have experience using the Echo Meter Touch to record bats. And I have accidentally recorded rodent ultrasound in the past when monitoring for bats with other detectors. Assuming the newborn vocalizations are ultrasonic I see no reason you cannot detect them with the EMT. In the settings you would just want to set the low threshold filter low enough, and you would want to keep Noise files.
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Our rodent'treadmill's bars show voltage but not affected animals. I cleaned all those bars as rat's urine is dielectric.
Do you have any idea that helps me?
Thanks
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Kevin Stevens Thanks Kevin
I mean the shock grids don't work properly and I've checked all part of our treadmill and everything is ok
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Alloxan or streptozotocin is a common agent used for induction of diabetes mellitus in rodents. From my experience, not all rats administered alloxan or streptozotocin developed diabetes mellitus. In most cases, this cannot be attributed to drug-/administration-related factors such as potency, underdosages, route of administration, wrong administration, etc. Are there studies that have investigated the resistance of these agents in some rodents? Are there scientific explanations or reasons for this resistance?
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What I have noticed generally is that, conventionally there's no standardised dose. Inconsistent outcomes have been repeatedly reported. I hope a study into the molecular mechanism is done to confirm the underlying causes.
This article relorted similar thing.
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Hello all,
I was wondering if someone could help me. I have read a lot of articles that mention using Betamethasone at clinically relevant levels in mice yet no one has mentioned the half-life. I have found a study with rodents yet nothing with mice.
Any help would be greatly appreciated.
Thanks
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Dear Erika Kathe Croft,
The following article recently published could be useful for you.
Thank you.
Yao, Q., Guo, Y., Xue, J., Kong, D., Li, J., Tian, X., ... & Zhou, T. (2020). Development and validation of a LC-MS/MS method for simultaneous determination of six glucocorticoids and its application to a pharmacokinetic study in nude mice. Journal of Pharmaceutical and Biomedical Analysis, 179, 112980.
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Hi,
How can I assess the intensity of a treadmill exercise in rats without measuring Vo2max?
I mean how can I know a specific protocol has a low or high intensity without measuring any factors in blood or using Vo2max?
Thanks all
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In addition to Thom's answer, this can also be useful: Physical Therapy, Volume 80, Issue 8, 1 August 2000, Pages 782–807, https://doi.org/10.1093/ptj/80.8.782
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During my outdoor trap tests with rodents I´m using infrared video cameras with motion detector. Afterwards I have to go manually through the videos and remove clips or scenes without animal to be seen which can be 90% of the recorded time. Than I put the clips together to one movie. Iwould prefer to have this step done automatically by some software. Friends of mine tried to program me a tool but didnt work. Is there something commercially available?
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Francis,
maybe I need to explain better.
One camera produces in one night 40 clips of 3 minutes, total something like 8GB. Most of the stuff there is nothing. Sometimes the camera gets activated but the animal passed before the recording started. Sometimes the animal is only 10 seconds to be seen, but runtime is set to 3 minutes. Every clip has date and time stamp. I do not loose any information if I remove all scenes without target animal. Time elapsed between shots can be determined easily. After reduction there is maybe 10 minutes of mp4 left, size 40 MB. That´s a reasonable size to store and to analyze behaviour. And if one would want to go later through the videos, no need to search 2 hours of mostly empty raw material, the compressed information is available. To do this summary manually takes minimum 2 hours. Boring and total waste of time. Some nights I do have set out 4 or more cameras. This means, somebody has to sit all day long and stare into a screen. I got a lady who does this first step for me. The interesting part starts when I get the edited movie. The direction of my research is the interaction between animal and trap. Trap shyness, trap resistance, humane killing, trip force, trigger function, time between first contact and catch etc. Things would be much cheaper for my customers if I could do this automatically.
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I would like to know in two different treadmill protocols, if we are going to reduce the treadmill velocity for rodents (14 m/min instead of 20 m/min) but increase the time of exercise (60 min instead of 45 min), then intensities of these two protocols are the same? (14 m/min, 60 min/day) (20 m/min, 45 min/day)
Thanks
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Good day!
These protocols are not equivalent, in the second case (20 m/min, 45 min) the running intensity is significantly higher with a sufficiently long exercise duration.
The values would be comparable with an initial speed of 15 m/min for 60 min.
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The idea is to use rodents and to induce HGT within their microbiomes, one high exposure group, one low exposure and one control. The extent of HGT will then be assessed. What size would my sample have to be at a confidence interval of 95% to indicate a statistical significant difference/correlation. Seeing as my actual samples would be the stool samples collected and the quantified microbiome rather than the actual rodent itself. The rodents are in actual fact vessel that house the actual "study sample".
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It is very difficult to understand your question anyway sample size is very important and host facilities as well
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Dear all
Does someone have a recent rodent phylogenetic tree (preferably in Nexus format or any other format usable in R) or knows a publication with a recent tree? Currently I am still using Fritz et al 2009.
Any help is greatly appreciated.
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In this paper (https://doi.org/10.1371/journal.pbio.3000494), you can find a time-calibrated tree for 6,000 living species of mammals. In addition, credible sets of mammalian phylogeny are available for download at http://vertlife.org/phylosubsets.
All the Best!
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I would like to know what is the best way to train mice in a virtual environment to perform behavioural tasks (to run consistently, to train them to reach a specific target without stops before to reach this target, and to navigate in 2D).
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Following.
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Hi. What kind of equipment do you use for taking pictures of gross lesions during necropsy?
I am looking for not too fancy digital camera. Can anyone recommend a camera that takes good quality images of gross lesions of small laboratory animals like rodents or rabbits? Thanks, Aga
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Hi doctor. You can used the mobil cam.
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For performing F-DOPA PETscan in rodents I have read it is possible to dilute entacapone pills and inject the solution at a dose of 10mg/Kg. Nevertheless, the solubility of the compound is very low. I have tried to dilute it in distilled water but soon after de dilution, it separates into two phases. Does anyone have a method for readily dissolving the compound?
Thank you in advance for your help
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Cationic surfactants would be more efficient for entacapone, but I am not sure about its safety
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I need to induce LTP in hippocampal acute brain slices (rodent) and measure extracellular fPSPs.
Different bipolar stimulation electrodes available on the market have different impedances (0.1 MOhm, 0.5 MOhm, 1Mohm, 1.5 MOhm, and 2 MOhm) and I am not sure about the most suitable (no info from the customer service…).
Could anyone recommend a particular impedance based on experience?
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Hi there. I use 0.1 MOhm tungsten electrodes. Using 0.5 MOhm or higher impedances didn't give good ltp results with my recordings. Good luck.
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Please, can you brief that a normal human echocardiogram device can be used to measure rodent heart status?
Thanks
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This work may be useful for you:
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What are the best ways of anesthesia and euthanasia in laboratory rabbits?
What are the best ways of anesthesia and euthanasia in laboratory rabbits and other rodents?
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Hi,
isoflurane is usually the best way to anesthetize rodents, it has many advantages compared to ketamine/xylazine injections and other chemicals. Recovery is very quick and you can adjust the flow and percentage in real time.
For euthanasia, the best way might be depending on your institution IACUC's protocols. I know that the preferred methods in France vs the US differ quite a bit in my area. All of these questions could be asked to them as they will provide approval of the animal protocol and useful advice for you in specific settings.
I hope this helps
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What are the different methods to evaluate circadian rhythm in rodents (rats and mice)? One method I found is Actimetrics which is not available in our lab. Can anybody suggest any alternative method
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An automatic open-field ambulation can register circadian rhythm in rodents:
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We want to harvest some mouse intestine tissue without any brown stuff in it. The challenge is that we would also like to perfusion fix the animal first, which makes the tissue stiff and contracting. I could not find any protocol around to deal with this issue. Any suggestions?
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36 hours is a pretty long fast, but mice eat their feces, so if you did not clean the cage when starting the fasting period that might have an impact. When I have to clean the intestines (it was not after PFA perfusion though), I just turn them upside down and use tweezers to empty them and rinse them. Did you try that?
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How would you distinguish peripheral monocytes from tissue macrophages with IHC? Specifically, I am interested in looking at rodent brain tissue. Any help is appreciated!
Thanks,
Dan
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I am leaning towards using CCR2, CD34, & CD117 as markers for peripheral cells... I think I'll stay with Iba1 for microglia... But considering F4/80 and CD68 in the future...
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What is the significance of the following solutions being used as rodent"s organ fixatives, between normal saline, citrate buffer and sucrose?
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I have used TeloTAAGG from Roche in brain rodent samples but without good results. It seems that the restriction enzimes from the kit are not effective in these samples.
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Sciencell has kits for measuring telomerase activities and telomere length on rodents.
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I have previously used Santa Cruz sc-8066 DCX antibody, which has been discontinued, and need a replacement for it. I will be staining rodent brain tissue with it.
Any recommendations would be greatly appreciated.
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Hey! Just to add to the great answers here:
My go to DCX:
Doublecortin-- shown in purple (Millipore AB2253)-- Guinea Pig--- (1:1000)
I hope this helps!
E
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We are planning to purchase a non-metabolic rodent treadmill. The budget is not high, but looking for a new one, motorized and with basic functions. Any recommendations about the features and estimated price?
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We used the very cheap wheel which is called squirrel wheel in our country, commonly used for hamsters.
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How we can use plant extracts in rodent control. What are the conditions and constraints that prevent the widespread use of plant extracts in the control of rodents.
Can the plant extracts be used to attract, repellent or sterilize rodents?
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I appreciate your response to my question
With my Best Wishes
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b, Frequency band width ( based on Niquest Frequency)e specs which are deemed to be necessary for rodent EEG recording with associated bio analyzers (temperature, pressure, Heart rate etc).
What are the pros and cons of the telemetry units.
Which one will be ideal for rodent sleep study ( tethered system or telemetry based) ?
The Specs will include
a, S/N ratio
b, Frequency band width ( based on Niquest Frequency)
c, Number of channels
d, automated software for analysis
e, vedio EEG recording, camera specs
f, no of rodent units per recording system
I request the elite scientist in this field to post your input.
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What are the pros and cons of the telemetry units? Any suggestions on selecting an ideal EEG recording and analysis equipment for Rodent sleep studies ? I suggest you take a look at the research that is being done in the field of EKG electrocardiogram machine learning based Signal Processing. The telemetry units thereof are autonomy generated communication measurements along with other relevant data that should be collected to detect any visible sign of genome mutation or terminal disease in a living being. In machine leaning, such telemetry units are used to solve visualization based unsupervised learning cluster charts. Good Luck. Beat, T.T.
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 I am getting very faint bands and cannot seem to increase the signal.
I have tried increasing the amount of protein per well, increasing the primary antibody concentration, 0.45 and 0.2 um nitrocellulose membranes, different transfer settings, different incubation times and different ECL detection kits. 
I would like to get in touch with someone who has had success using proBDNF (5H8) or any tips or tricks to detect BDNF by western blot. 
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Although the problem didn't get solved in my case so far. But I read transfer conditions can help. Do try 60 min wet transfer at 100V. The samples should be electrophoresed using 15%gel. My antibody N20 santa cruz could detect 15ng of mBDNF peptide loaded as control on the gel at 14kda , however didn't detect any mBDNF at 14kda for hippocampal lysate and N2a cell lysates loaded. Best of luck to you!
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I'm planning to do an 8-isoprostane ELISA (using the Cayman kit) with some guinea pig tissues (adult liver, fetal liver, placenta).
Just wondering if anyone has done this assay with similar tissues and has any advice about what kind range of tissue dilutions to start with for similar tissues. I'll still be troubleshooting to find optimal concentrations to run for each type of tissue, but it would save time and reagents if I can start with a narrower range.
Thanks!
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Special 3 ml dilution with 100 mg of tissue
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I am looking for a good surgical monitoring system for mice, mainly ECG recording. For different suppliers it is mostly the same Scintica Rodent Surgical Monitor+ system. Does anyone know a alternative with a more reasonable price?
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Dear Jef,
thank you. This one I know. Unfortunately seems their is no real competitor on the market.
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Hello everyone. I am looking for a good protocol for isolating mitochondria from brain, but I want to separate synaptosomal mitochondria from non-synaptosomal mitochondria in hippocampus. I know some protocols for this, but they use the whole brain or some areas bigger than hippocampus. I absolutely know that I must pool several hippocampus, but I want to know wheter it is technically possible or not. Could someone share with me his experience? Thanks!
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Dear Pérez-Hernández
I have attached a protocol for synaptoneurosome isolation along with a reference paper. This protocol doesnt need neither ultracentrifuge nor percoll.
Hope this is helpful for the project.
Regards, 
Yogesh Kanna Sathyamoorthy
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Intra-nuclei brain administration and also systemic administration?
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Mice or rats? A mouse is 30 g, a rat about 300 g. There are web calculators for BSA:
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reason to do that?
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Hii Respected Paola
Thank you for your suggestion...yes, I am infusing drug for behavioral anxiety study..
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Currently, I am trying probe for p-FOXO3A in rodent skeletal muscle. To date, I have tried countless times to get a signal adjusting what I thought would benefit, with limited success. The homogenization buffer has been prepared two different ways with both ways having been published successfully. I did find interestingly that transferring at 100v for 60min was more effective than 90v for 90min.
Running: 80v - 147min
Transfer: 90v - 90min or 100v - 60min
-Towbin Buffer with no SDS
-Towbin Buffer with SDS modified to 15% methanol, published technique
Ponceau S check, rinsed with ddH2O
Blocking: 5% BSA - 1hr and 7% NFDM 20min or 1hr (either in 1x TBST, 7.4pH)
Washing: 1x - 5 min, 25 mL TBST
Primary incubation: 2% NFDM, 1:1000, 10 ml or 5% BSA, 1:1000, 10 ml - made fresh - 18 hrs - 21 hrs. 4-6 degrees C
Washing: 3x - 5 min, 25 mL TBST
Secondary: 5% NFDM, 1:2000, 10 mL, 1hr Room Temp
Washing: 3x - 5min, 25 mL TBST
Imagine: ThermoScientific Pierce ECL Prod# 32106
My images are basically with no background to a vague signal or just nothing for a signal.
I have checked BSA blocking (which provided the vaguest images), 7%NFDM which provided nothing for an image. I have tested for protein blowout recently with back to back membranes and found some blowout but of the smaller proteins and not near the 90kD of p-FOXO3A. I am at a loss. All attempts have been using Cell Signaling antibodies across two lots. Any help would be most appreciated.
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Hey,
do you have any possibility of a positive control? Your protocol seems fine. Did you try an antibody from a different source, not different LOT?
Best
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Mice are naturally nocturnal but most of the lab behavioral tests are conducted in the day time. Is this confounding? Therefore when is the best time to do behavioral test of mice/rodents?
Thanks in advance,
Monokesh
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Great answers! I think I got some ideas. Can anyone suggest any paper to follow?
Thanks all.
Monokesh
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How can I prepare a pellet high fat diet for rodents that keeps a firm consistency?
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If you want to determine feed intake, then pellets are to be preferred. Also then the animals can not select individual feed ingredients.
You can calculate fat content from ingredient composition or determine it chemically.
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We have been doing dissections of small arteries and collateral arteries in rat. We have been dissecting out and splaying open the femoral artery and one of its major branches (we usually refer to it as the profunda, as that is the human analog). The blades of the spring scissors we use are just a little too broad to fit effectively - it sort of works, but stretches the vessel a little to make a cut. Any suggestions? I have been looking at these as possible replacements:
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Hi Dylan,
In our lab we dissect mesenteric arteries and coronary atteries with relative ease, using scissors with the same specifications as product FST Item # 15000-03. Best of luck!
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I am interested in any published data on how percentage of different age stages (juveniles, subadults ...) change depending on the type of the taphocoenoses etc?
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contact to Prof. Rajeev patnaik, dept. of Geology, Chandigarh University ( India)as he is working on siwalik rodents set up the bichronology of Siwalik rodents.