Rhizobacteria - Science topic

I am interested in knowing if there is any precedent in the scientific literature on the use of plant growth-promoting bacteria obtained from the rhizosphere that have been inoculated into the leaves of plants with good results.

Thanks in advance for the collaboration...

Mention the purity and brand of the chitin to be amended in the medium for chitinase production by rhizobacteria.

There is an open research grant call which requires the collaboration of researchers in the Global South and Danish researchers. Any Danish researcher working on Climate Action, particularly on Climate change resilience and adaptation to get in touch with me on baloo@uoeld.ac.ke. My area of research is on plant-microbe interactions, rhizobacteria, and plant growth promotion. Thank you

We first isolated some PGPR. We carried out a gram test, and we found they were gram-positive bacteria. Then, we induced these bacteria with EMS to get mutants.

After mutation, we grew mutants, and we found greenish-blue pigment on nutrient agar. We examined the gram type of these mutants, and they were gram-positive too.

Could the other bacteria, except for Pseudomonas aeruginosa, produce pyocyanin as the result of mutation?

Thank you so much!

The best method to evaluate the potential of siderophore production in rhizobacteria under in vitro conditions?

Qualitative, semi-quantitative, and quantitative evaluation...

isolation of rhizobacteria from turmeric rhizomes but there is the contamination of fungus.

want to isolate rhizobacteria from soil and hence required fungal growth inhibition hence need to add flucanazole in media

want to isolate rhizobacteria from soil and hence required fungal growth inhibition hence need to add flucanazole in media

Hello everyone,

We are planning to study how plant growth promoting bacteria are affecting various plants (eg. wheat, barley, etc.). We would like to coat the seeds with bacteria. We prefer film coating. I can't find any proportions of seed, inoculum, carrier materials and sticking agents.

Could you please share the recipe you are using for your research? We prefer using cellulose or lime as a carrier material.

Thank you in advance.

My question is that if we found that in the soil have some bacteria which produce PGPH then how we will find it out to know that only this bacteria have the potential ability. secondly how we will use it is a biofertilizer??

People working on the field of Biofertilizers (PGPR and AMF) and want want extend their networks can comment by YES or if possible indicate the projects or topics of their research and start following each other.

I am lay in this subject. Is DNA sequencing the best way to identify the different types of soil bacteria present in soil samples? Ideally, I'd like to find a technique that allows me to identify all different types of bacteria (from those that could be identified) present in a given soil sample. Thanks.

I need articles related to this subject, especially the rhizobacteria of durum wheat.

Hello,

-So I have a set of bacterial communities extraced from rhizospheric soil in both saline and control environments for two different cultivars of plants, one is tolerant to salinity and the other is susceptible.

-I did ordination (N-MDS)and got the control and treated separated on first coordinate, but cultivars closer together in the second coordinate.

- I got p-value p=0.001, whic is good, indicating diffrence.

-However, i got R^2 values: axis1 = 0.9521, axis2= 0.0005229.

What does R^2 mean? what values indicate that my data is good. Is it a strong test for my data?

What final concentration of 27F and 1492R primers and Taq polymerase we use for the amplification of 16S rRNA region of rhizobacteria.

The final concentration of Primers???

The final concentration of Taq polymerase?

Experiment sample : Rhizobacteria.

After screening and isolation and the extracted of bacterial DNA by using 16s ribosomal technique to get sequencing result (in the form of nucleotide sequence).

Recently I work on Isolation & Identification of rhizobia bacteria of chickpea & groundnut.I want to know which rhizobia bacteria can create nodule of chickpea & groundnut/peanut.Please anyone help me so I will gratitude to you.Advance thanks.

Does rain water drain away root associated bacteria or soil bacteria? If so, what can be the percentage of washed out bacteria? Any study?

Thanks in advance!

Hi..I would like to detect siderophore production by rhizobacteria. I have rhizosphere soil samples to test and have used a CAS overlay method that hasn't worked since the original colour was red orange so a change to red orange cannot be seen. I am thinking of using an assay involving HDTMA but some Shweyn and Neilands say that gram positive bacteria are sensitive to this detergent. Please advice on the best method to use if testing from raw soil samples.

I am currently working on isolating and screening rhizobacteria. One of the properties am concerned about is nitrogen fixation ability and so I am growing the bacteria in nitrogen free media. Literature suggests that the colour of the medium should change from green to blue. However, am getting colour change from green to yellow and this is quite confusing.

This is not a question. This is the Map of "Heavy metal plant rhizobacteria" topic.

file topic_report.docx = 20 topics from 844 articles which have words

ts=(heavy AND metal* AND plant* AND (rhizobacteria* OR (rhizo* AND bacteria*)))

in their titles or keywords or abstracts. Each topic is represented through 20 words and 20 thrases with which it is discussed in these articles. Really this terms are the names of methods, objects, properties, laws and so on for topic in question. In addition each topic has quotes from two articles in which it is most manifested.

file a1_basic.xlsm - articles with basic knowledge on the topic

file a3_novelty.xlsm - articles of last years potentially with novelty

We will be grateful for the feedback.Feel free to ask an analysis of a specific topic.

===

The Experience of feedback from graduate students and researchers:

sometimes they find articles and methods that they have omitted,

reading the terms of the topic_report file makes it possible to initiate

ideas or directions for further research that have been forgotten or never come to mind.

I need to work with disinfected soil, does putting this soil in an autoclave make it lose these characteristics?

Is there any other techniques to obtain disinfected soil, without any microorganism?

Greetings

I am looking for papers regarding pgpr that could be used in hydroponics.

Which articles would you suggest?

I am interested both in pgpr that could be added in the rizosphere and the phyllosphere.

Thanks in advance

suggest the best api kit for gram negative soil bacteria or rhizobacteria.

The roles of rhizospheric bacteria in case of terrestrial plants are well studied. They establish 'give together' condition and show any of mutualism, commensalism, amensalism or pathogenic relationship. They are considered as the hotspot of microbial activity often helping in multifaceted ways in nutrient mineralization & aquisition, plant growth promotion, developing 'immunity' against harmful microorganisms. Such activities may be mediated by IAA, siderophores, ACC deaminase, bacterial antibiotics produced by them.  By there is huge knowledge gap in case of aquatic macrophytic rhizobacteria. Although the role of many macrophytes have been attempted in phytoremediation of heavy metals and other toxicants as well as in nutrient (nitrogen & phosphorus) removal and wastewater treatment but the real role of the rhizobacteria still remains neglected. Indeed they play the pivotal roles interacting with the macrophytes. The actual mechanisms of action and bacteria-macrophyte interaction need to be unveiled which would be helpful in understanding the phytoremediation and the nutrient reclamation/removal process more clearly. 

All the RG colleagues are requested to contribute in discussing the issue with thoughts, experiences and resources. I look forward to an enriching and though-provoking brainstorming on the RG platform.

Somebody claims that there some selfish rhizobia which take benefits from the host but do not induced nodulation at the host. I did not get enough evidence about this. Could anybody help me by giving some articles about this? 

I'm studying bacteria isolated from cherry rhizosphere. I would like to perform some experiments about the interaction of the isolates with cherry trees, however, cherries are difficult to growth from seeds, apples are difficult too as they need also a long process before germination. 

Any suggestions?

thanks

Plant roots exude a variety of sugars, organic acids, etc. that attract and feed fungi and bacteria.  Many of these microbes are know to protect the roots from attacks from other fungi and bacteria.  Those that grow along the growing root are generally designated as 'rhizosphere competent'.  However, in potato there are both roots and rhizomes.  There are several pathogens that attach the rhizome and the potato tuber.  So, the question is whether or not the rhizomes or tuber exude chemicals that would support microbiological growth in the same way the root does?  If so, this might allow for the discovery and application of microorganisms to the root zone of potato that would protect the rhizome and tuber from infection.

Hydroponic systems currently employ normalized, sterile, water soluble fertilizer solutions which are highly effective for growing high quality crops quickly. However, these mined salts are not sustainable and must be replaced with organic sources. As we know, plants do not utilize organic compounds in the root zones for nutrition, and insects and microorganisms must break these compounds down to usable inorganic plant nutrients. Would it be preferable to create a process by which consistent compost material is harvested of its degradation products for use in hydroponics, or would it be best to employ fertilizers with mixed organic and inorganic nutrients to a system pre-innoculated with beneficial mycorrhizae and rhizobacteria? Research has shown that hydroponic cultivation can impart higher nutritional value to edible crops when compared to organic and conventional cultivation. However, it has also shown that aquaponic cultivation supplemented with inorganic nutrients performs even better on yield. Could this be because soluble nutrients are supplied alongside insoluble ones in a living system? Thank you in advance for your thoughts on the matter.

lakshmi mundkur, microbiologists

I have inoculated Rhizobial isolates to newly cultivated soybean plants in a controlled condition. They formed nodules but there is a huge variation among plants dry weight. More important thing is that control plants got more weight than inoculated nodulating plants  in agar and Fahraeus N-free medium in glass house. 

I found in many articles that bacillus sp and pseudomonas sp are the principal nematophagous bacteria which formed too the prncipal species of PGPR?can any one explain to me please?

Hi everyone, I am using Agrobacterium rhizogenes to induce hairy roots.  i wonder if (1)  i culture unclear hairy roots (i.e. still contaminate with A.rhizogenes) in liquid medium, will A.rhizogenes suppress hairy root growth? or (2) antibiotic, if added to liquid media, will suppress hairy root growth?

Thanks so much for your help!

hi i'm studying the potential of somme rhizobacteria to solubilize phosphate and producing AIA , and i want to study the potential of solubilizing potassium (KSB) so in many research they use the aleksandrov medium amended with insoluble source of potassium, so my question can you give me the name of a commercial product containing an insoluble form of potassium or any thing can help me in this study. 

How many days are need to create root nodule by rhizobia sp.?

Long term storage of bacterial culture other than lyophilization

I have got a lot of colony of bacteria from soybean root nodules aseptically but they are not behaving like rhizobia, for example, they grow fast, remain attached with conical flax. I am trying the best for aseptic isolation and inoculation. Give me any suggestion.     

what species are fixing N2 symbiotically with soybean?

I wonder if I could isolate mycorrhizal spores from rice soil and rhizosphere samples that I got with the initial aim of isolating plant growth promoting rhizobacteria. Some experience on that?

Sampling soils from natural o reserve areas must be an opportunity to get some rhizobacteria adapted to enviromental stress with respect to agroecosystems or it is not relevant to begin a prospecting project?

Thanks for comments or suggestions.

what is the best method to evaluate the plant growth promoting potential for my strain on arabidopsis thaliana ; inoculation of seed or soil? and in vitro or vivo?

What are the different oil based formulations developed for delivery methods of agricultural beneficial microbes (like pseudomonas fluorescens/bacillus species?  

plant extract oils such as neem , pongamia , groundnut oils are we wanted to test. 

I shake the root until loosely free soil has fallen off, then I wash the root with 10 ml saline solution with sterile brush. Then from the washing solution, I isolate the bacteria. Is this method right or not? I am isolating from Halocnemum and arthrocnemum root.

My interest is in innoculating pot soil with PGPR, possibly with the most inexpensive method.

Pseudomonas aeruginosa is an oppurtunistic pathogen still we use it as an efficient Plant Growth Promoting Rhizobacteria. How can we check the pathogenecity of P.aeruginosa in laboratory by applying simplest technique

If someone can help me in getting a Rhizobial strain that infect chickpeas (Cicer arietinum) to produce N2 fixing nodules and it should have GFP/GUS/DsRED or anything like that as a visualization tag.

I am trying to isolate heavy metal resistant Potassium solubilising plant growth promoting rhizobacteria which can promote plant growth during heavy metal stress conditions.

I am preparing CAS agar medium for testing the siderophore production of antagonistic rhizobacteria. I am following the procedure of Schwyn and Neilands 1987 which is elaborated in detail by Louden et al. 2011. The recipe says that after preparing the casamino acid solution extract trace iron with 3% 8-hydroxyquinoline in chloroform. Can anyone tell me how much volume of 3% 8-hydroxyquinoline in chloroform should be prepared and after I have prepared the solution how can I extract iron from casamino acid solution. I need the methodology.

nodulation inhibition by pgpr...

Can you recommend me some article about that?  thank you

I am working on anastomosis group typing of local isolates Rhizoctonia solani and need tester isolates. Can someone guide where to get these tester isolates?

What are the quantitative methods for estimation of ammonia production by plant growth promoting rhizobacteria (PGPR)?

Plant growth promoting rhizobacteria such as Pseudomonas spp., Bacillus spp., Enterobacter spp. etc are used as bioinoculants to improve crop growth and yield. Will it be OK to term them as plant-probiotic?

I am confused why do we have to run HPLC and GCMS at once. I would like to know methods on how to extract gibberellin from microbes. Most methods use GCMS whereas there are also other method using HPLC.

A few locally isolated rhizobium were used mainly in improving crop yield. I'm inspired by the fact rhizobiums could transfer genes as efficient as agrobacterium. However, the local rhizobium strains are yet to be identified until species level. Is it possible that I try out these strains to transform my plants?

It is known that for Bradyrhizobium genus, the 16S rDNA gave no differences between the different species and to identify a new isolate, we have to check other genes, such as recA, atpD, glnII and others as well as DNA-DNA hybridizations in case it is different from the known described species. But is it also mandatory for the fast growing rhizobia such as Ensifer (ex-Sinorhizobium) strains? Do you think I have to go on with housekeeping genes sequencing even the total 16rDNA and partial nodC sequencing gave 100% similitude with a reference strain?

I need to isolate chitinolytic rhizobacteria, so I wish to know the exact composition of the medium used to isolate it.