Questions related to Retinal Diseases
Hi, I am aware of anti-zpr3 (from ZIRC) stains rod and double cone outer segments, but I have no clue, what is the function of ZPR3. Could anyone help me to find answer to my question.
I am currently trying to establish a protocol to differentiate iPSCs to Retinal Pigment Epithelium. I have set up an array of conditions to establish which is the best method to establish the cells. Some cells very early undergo epithelial to mesenchymal transition while others have came along nicely and are polyclonal. I have previously tested and the cells are positive for RPE markers including RPE65, ZO-1, VEGFR. However I am having difficulty in that my cells are not showing any signs of pigmentation. I am currently culturing my cells in Miller Media with 1% FBS. I plan in the forthcoming weeks to perform IF and RT-PCR to determine if the cells test positive for MITF and PEDFR.
However in the meantime does anyone have any suggestions as to why my iPS-RPE may not be pigmenting and how to induce such? Any comments are widely appreciated!
In a modern ophthalmic setup assistants may send to the doctors images over the internet to diagnose retinal diseases. Which model do you think is the best for this aim?
What do you use for screening of retinal diseases except OCT and other expensive technologies? In developing country they are not available to cover all population. Moreover, if the territory of the country is huge.
I am currently working on the effect of salinty in taurine production for brakishwater fish. But, I am also interested on the taurine synthesis pathways in fish. Are you familiar with taurine receptor genes? Or any other enzymes aside from CDO amd CSD that aids in taurine production?
Please I need some help with possible differential diagnoses and/or management plan. Fundus photo of an active 47year old male African, Right eye. VA=CF, exotropia approx. 30o. Lens, cornea, vitreous are all normal. Good pupillary reaction with mild RAPD, IOP 14mmHg. History of decreased vision since childhood. No history of trauma, diabetes, HIV, or hypertension. The left eye is normal.
I have been using a procedure for mouse eye fixation in paraformaldehyde. We remove the mouse eye after sacrafice. We immediately put it in 4% pfa for 30 minutes. After that we punch a hole in the eye, we do not remove the cornea or lens. After fixing for 4 hours, we put it in 10% sucrose for an hour, 20% sucrose for an hour, then 30% sucrose overnight. We then put the fixed eye into a cryomold filled with OCT mixture without sucrose. We freeze the tissue on dry ice. Sometimes the retina is perfectly sectioned, other times, it is extremely detached and has cells missing or shrunken. Any advice would be helpful. Thanks
I am looking for antibodies for CNGA3 and CNGB3, important channel proteins involved in the cone phototransduction pathway. Has anyone found antibodies for these proteins that work well for Western blotting of mouse retinal extracts?
45 year-old women was admitted to OPH d/t retinal vasultis. She has a plan to recieve long term and high dose steroid. But, her chest X-ray showd inactive tuberculosis and no history of treatment of TB. Should she take INH for prevention of occurence of acvtive TB ?
My first choice is vitrectomy (including A-V sheathotomy & internal limitting membrane peeling) in case have below condition:
1 Artery on vein
2 occlusion not in disc or on disc
3 short duration from onset (less than 1-2 month)
4 Visual acuity less than 20/30
5 Foveal macular thickness more than 450 micron
6 Patient accept operation
Second choice is Anti-VEGF therapy or Subtennon steroid therapy.
And I never perform photocoagulation for non perfusion area in initial therapy.
Please tell me your recommend therapy and your opinion.