Science topic
Retinal Degeneration - Science topic
A retrogressive pathological change in the retina, focal or generalized, caused by genetic defects, inflammation, trauma, vascular disease, or aging. Degeneration affecting predominantly the macula lutea of the retina is MACULAR DEGENERATION. (Newell, Ophthalmology: Principles and Concepts, 7th ed, p304)
Questions related to Retinal Degeneration
I am working with mouse retinal samples which are lysed in RIPA buffer. I was looking to use lamda protein phosphatase but the components of RIPA inhibit the phosphatase. Any recommendations on a phosphatase to use? I will be running western blots on these samples.
I am doing immunofluorescence staining on retinal sections from RCS rats to assess GFAP. As shown in my attached image, the GFAP staining is not consistent with most literature I've seen with retinal GFAP. Typically, I see long connected processes throughout the length of the retina following injury. But as shown in my image, I get short processes. If anyone has any information on if I am doing something wrong or explanation about the images, I would greatly appreciate help.
Here are all the details that might help:
- RCS rats are a retinal degeneration model, they were p90 when euthanized
- fixed with Davidson's (formaldehyde based), paraffin embedded, cut at around 7-10 microns (I think 7)
- did heat antigen retrieval using citrate buffer at pH 6 (I know I probably don't need to but I have to for a co-stain I will use in the future)
- blocking buffer is 5% goat serum and 0.1% triton-X in PBS, GFAP antibody is rabbit polyclonal (dako/agilent z0334) diluted 1:1000 in blocking buffer (I did a titration all look similar in terms of structure of GFAP), and secondary is goat anti-rabbit conjugated with alexa fluor 594 (invitrogen, A-11037 ) diluted 1:500 in blocking buffer
IT is a picture of the fundus. I am not sure about what is this indicative of and what landmarks should I take into consideration. I have acquired a lot of such images but I am not able to interpret them.
optical coherence tomography (OCT) is used with considerable success to study retinal degeneration. I am trying to evaluate the status of biomarker discovery of neuro-retinal degeneration in Macular Degeneration using OCT and related imaging systems
My research is related to cell line study in diabetics and retinopathy, does anyone know of any animal activity labs that do these kinds of experiments?
retinitis pigmentosa is the most common inherited human eye disease resulting in night blindness and visual defects.
as a biomedical engineer i like to know how can we help this patient by using ips cells.
thanks for sharing your information with me.
I want to induce retinal degeneration in vitro in a specimen of goat's eye. Which chemical can be used for the purpose?
I am interested in transcorneal electrical stimulation and I'd like to start an experiment with this tecnique, but I am having troubles in finding mice corneal electrodes for this purpose.
Thanks in advance for your help!
Hi everybody,
Can anyone describe the exact protocol to set up this animal model of retinal degeneration?
My questions are as simple as:
(i) When you put back to normoxia the mice at P12 (after the 5-days period of 75% hyperoxia), do you just take the cage outside the oxygen chamber back to the atmosphere normal room conditions or do you keep them in the cage and set up the oxygen rate to normoxia?".
(ii) Could you provide me a list of the material I need to set up this model?
(iii) Are there any trick I should know before starting?
(iv) Are C57BL/6 sensitive to this treatment?
(v) How do you manage the death of the feeder mother? I mean do you specially buy some feeder mice for each experiment or do you use some feeder mice from you animal facility?
Thanks a lot for all the answers to those questions and for all the détails you could bring into my knowledge!
Juliette
we are conducting a lab research to test a type of stem cells in the retinitis pigmentosa. I want to know which animal model is the most suitable for the research.
I know it may depend only upon the choice of the research team but I need to know which aspect should I look for in the model?like what would make the research process less problematic. What will give the same result that will be in human ? etc
I want to better understand this from the physical point of view. Thank you in advance!
I have been using a procedure for mouse eye fixation in paraformaldehyde. We remove the mouse eye after sacrafice. We immediately put it in 4% pfa for 30 minutes. After that we punch a hole in the eye, we do not remove the cornea or lens. After fixing for 4 hours, we put it in 10% sucrose for an hour, 20% sucrose for an hour, then 30% sucrose overnight. We then put the fixed eye into a cryomold filled with OCT mixture without sucrose. We freeze the tissue on dry ice. Sometimes the retina is perfectly sectioned, other times, it is extremely detached and has cells missing or shrunken. Any advice would be helpful. Thanks
I am planning to do injections in mice with Metripranolol. Found that the drug is practically insoluble in water/saline. What do people using this drug use as a solvent when they do injections? Thanks in advance for your answers.
I'm mostly interested in early life disorders, but late life disorders may also be of interest.
I am doing western blotting for synaptic vesicle protein and it gives double band with smeared pattern (manufacturers details).
I am also getting the same pattern in wild type mouse but this specific pattern of bands is absent in retinal degeneration model. I am getting a single band. I am not sure how to compare the two. This specific protein is glycosylated in nature. Any suggestions?