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# Repair - Science topic

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Research studies including randomized controlled trials often have a time-to-event outcome as the primary outcome of interest, although competing events can precede the event of interest and thus may prevent the primary outcome from occurring - for example mortality may prevent observing cancer recurrence or may preclude need for reoperation in patients who undergo surgical repair of heart valves. Researchers often use Kaplan-Meier survival curves or the Cox proportional hazards regression model to estimate survival in the presence of censoring. These models can provide biased estimates (usually upward) of the incidence of the primary outcome over time and therefore other models which address competing risks, such as the Fine-Gray subdistribution hazards model, may be more suitable for estimating the absolute incidence of the primary outcome as well as the relative effect of treatment on the cumulative incidence function (CIF). My question is whether the Nelson-Aalen estimator is a reasonable option for estimating the hazard function and the cumulative incidence of the outcome of interest in the scenario of competing risks and if so, why is this a preferred approach over the Kaplan-Meier estimator?
Google search for this title and I hope it helps you. It's in the attachment. Best wishes David Booth
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In a recent lecture during the construction of a temple in our region, it was shown that one of the rock got cracked during the excavation done for the foundation work and the rock is being repaired by stone stitching (crack stitching) technique and wanted to know more about the same.
Thank you sir
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How can I measure (or calculate) the below fluency?
Could anyone explain to me with an example of two people?
When I read the paper, it does not explain how I can calculate it exactly?
Thanks a lot
(1) Speed fluency
A. Articulation rate:
mean number of syllables per minute divided by mean amount of phonation time (excluding pauses)
B. Speech rate : mean number of syllables per minute divided by total time (including pauses)
(2) Breakdown Fluency
A. Mean length of pauses per 60 seconds
B. Mean number of pauses per 60 seconds (clause-internal versus clause-external)
(3) Repair fluency
A. Repair measures : mean number of partial or compete repetitions, hesitations, false starts and reformulations
B. Mean number of filled pauses (e.g., em and er)
(4) Dialogue only measure
A. Number of turns
Dear Mee-Jee Kim,
You can find answers to your questions about speech and articulation rates in our study: https://dergipark.org.tr/en/download/article-file/1200953 When measuring these parameters, it is very important which time you will refer to as the pause time (e.g., pauses short than 250 Ms for articulation rate).
Good luck.
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This question is spurred by the different effects of BRCA1/2 vs. PARP.
DNA repair pathways are involved in maintaining genetic stability and, in this respect, repair factors are to be considered as tumor suppressors. This applies to the BRCA1/2 genes, for which inherited mutations in one allele predispose to cancer. There is no paradox in the fact that PARP inactivation leads to the death of BRCA-deficient tumor cells. In this case where homologous recombination is impaired, cell survival relies on PARP-dependent backup repair processes. Apparently, here, PARP promotes tumor development but even in non-tumor HR-deficient cells, survival would depend on PARP. We just take advantage of this synthetic lethality phenomenon due to HR deficiency to kill cancer cells.
Anyway PARP functions are not restricted to DNA repair and it is possible that in some peculiar circumstances PARP may rather promote tumorigenesis.
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I have four female carolina sphinx moths as pets and one of them was unable to fuse her proboscis after emergence. Is it possible to help her repair her proboscis to enable her to continue to drink?
Hi Ash; What are the environmental conditions for rearing? Sounds like you are really serious about these moths in you have multiple generations at hand.
Manduca lays its eggs on my tomatoes. Murder is the only recourse in So. Calif.
In the 1970s I and a couple of students did a project on the thermal biology of Hyles lineata. George Bartholomew and Bernd Heinrich (then a grad student) provides some data and advice to the effort. Many warm nights in the desert poking moths and attracting police. Never published it, but it was good stuff.
Cheers, Jim Des Lauriers
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Can anyone help me with finding a manual for the Zeiss Jenalumar microscope.
We found a 1983 model in the stores and as far as we can tell, the optics are far better than anything we could afford to buy today. Unfortunately, the instructions manual is missing and Zeiss haven’t answered my request for a new one. I managed to find the repair manual on the internet, but not the instructions for use.
Any chance to share it us Andrew? Regards!
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Is it before 72 hours?
Before 1 week?
Before 10 days?
Rarely in clipped injurlies before 2 week??
If there is a drain and no distal obstruction, I will wait for 6 weeks. The general condition of the patient will play a role in surgery timing too.
Otherwise, I will go to urgent surgery, when none of the three above circumstances are not well fulfilled.
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Recently, the XPS instrument in our lab underwent repair and maintenance. The difference that I can find now is that I am unable to get a clear focus of my sample (when it is inside the analysis chamber) as I used to get earlier. This is degrading the quality of my plots with no clear peaks to be seen. Any troubleshooting suggestions would be highly appreciated!
If you can't get any clear peaks, that sounds pretty much like a voltage issue, so either the analyzer energy or the retarding ratio might be not well-defined anymore. I suppose checking with a multimeter whether (a) a lens is short-circuited (can be done without venting) to grounding/other lenses or (b) all lenses are still contacted (requires venting).
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Is measuring Bst Polymerase activity (by comparing it with NEB Bst Polymerase) using SYBR and FLUOstar Omega machine (because our RT-PCR machine has been broken and I'm not sure when it will get repaired).
FLUOstar Omega is a microplate reader that have function for incubation with temperature up to 65 degree C (LAMP reaction need 60).
Here is the list of detection modes this thing have:
UV/vis absorbance
Fluorescence intensity - including FRET
Luminescence (fl ash and glow) - including BRET
Time-resolved fl uorescence - including TR-FRET
AlphaScreen®/AlphaLISA®
does anyone have experience like this?
any opinion/suggestion would help alot.
Yes, it is! Just get in touch with our APAC Support Specialist Joko Logis
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Our lab purchased the Spectramax ID3 and have encountered an error in the built in computer due to an issue connecting to the hard drive. Has anyone had experience with this and know of a resolution or the cost of repair?
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Nowadays mass spectrometry instrument vender is not providing repair or service manual. Third party service provider is not going to teach you how to repair the instrument either. Communicate with other user is very helpful.
Try ISOGEOCHEM if you not already did.
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Is it necessary to repair the missing residues on AutoDock Vina if they aren’t my selected residues? Would it affect my docking results?
Dear Yie Woon Chong, I agree with Sutanu's answer. Docking in Vina is usually done in a semi-flexible way, i.e., the receptor is rigid and the ligand is flexible. In this scenario, residues far from the binding pocket won't affect the results. The same even in the case of "flexible" docking in Vina - it still keeps the protein rigid, and only a few selected residues are allowed to move, so, again, distant missing residues won't disturb the results.
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Dear limnologists - marine scientists
I am looking for a sampler that can collect integrated water samples in the 0-20m depth range.
In our lab we have an old, mechanical Schröder bottle which unfortunately stopped working and may no longer be repaired. We'd prefer to replace it with an equally simple, mechanical device, but I'm having a hard time finding one.
Do you have any suggestions?
F. Lepori
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Montezzia fractures
In Monteggia fractures radial head usually reduced closely after reducing the ulnar fracture and annular ligament is rarely required.
In cases of neglected or chronic Monteggia fractures with chronic radial head dislocation, annular ligament reconstruction may be required to restore the radial head stability.
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Is it possible to predict double strand breaks (DSB) (or repair??) using only ATAC data?
Thank you both Nobuyuki Hamada Wei Zhou for your insightful response. We have in vivo radiated livers harvested 24h after radiation stop, and the ATAC-Seq is performed in bulk tissue. We have surrendered our attempt to predict DNA damage in this material. However, I believe it would be possible if the experimental design is optimal (time point, cell type, scATCC-Seq, and adapted bioinformatic tools). I really liked your idea Wei Zhou regarding the mismatched reads, but I believe the background noise could make it difficult when bulk tissue is used.
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A 23 year old female sexually active was diagnosed with MRKH has associated RVF. MRI shows incomplete vestigial uterus and absent vagina. Examination reveals RVF. Bowel (ileal) neovagina construction and RVF repair done at the same time. Are there any alternative approachs?
Here is one similar paper.
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after 2 month of age 50WK or above 5 Kg?
Interesting
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We are designing a strategy to make CRISPR knock-in intestinal organoids using Lentiviral delivery (as other transfection method are very inefficient in intestinal organoids). For designing the guide RNA, this seems straightforward, and we are planning to use the lentiCRISPRv2. However, for the repair template we are struggling to figure out which backbone would be best. The worry is that cloning our repair template with homology arms into a constitutive Lenti plasmid would result in random insertions of the homology arms and tag instead of being used as a repair template. Does anyone have experience doing CRISPR knock-ins using lentiviral delivery that might be able to give some advice? Thanks!
You could use AAV to deliver the repair template. Or alternatively, use electroporation to deliver Cas9/sgRNA(RNP) and repair template DNA (plasmid or ssDNA).
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there are numerous drugs for newly diagnosed, treatments are needed to enable repair and remyelination.
thanks wolfgang, i've been considering probiotic strains previously
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Is anyone familiar with DIY repair of the Typhoon (Amersham/GE/molecular dynamics) lasers canners? Can't find any hints online. Our current issue with it is a faulty shutter cable.
BTW, in case anyone is stuck with a "firmware" error in the older machine (ours is 2007 and is beyond repair), one can open the PC box (in 2007, it was a Pentium III) and connect a VGA monitor and a keyboard to the PC board, and read the error code that comes up during start up. Ours shows a shutter cable problem, which is where I am stuck now.
In the past, I have replaced faulty fans, and the fast stage drive belt. All of these "problems" were quoted by GE to be fixed for between 5K and 15K, at which point the machine has been abandoned, and I've been fixing issues with it for the last few years on case by case basis.
That's very generous of you Andrey, thanks! Unfortunately I'm in the US - Oregon, but we could pay for phone / web support. My email is iall@pdx.edu, thanks again.
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Recently we found in a mineral processing mine that column was damaged due to corrosion to a significant level. In this it seems base plate was also corroded. This requires almost entire replacement. Do we have any alternate way of repairing the same.
In some case the lower portion of columns was encased in concrete to prevent this type of failure, whether making lower portion as a composite column is advisable as this will change the entire behaviour.
The interesting part in this is that this is bidirectional corner column supporting two platforms above. Inspite of so much damages in foundation the structure is still working and suggest the very high factor of safeties in mining structures.
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Hello every one
my question is about in vitro exposure of blood samples (blast cells) with ionization radiation to repair DNA damage.
The best
leukocytes appeared 15 to 30 min after tourniquet release. Animal works
revealed showed that postischemic effects on DNA peaked
at 1 to 6 h after tourniquet release and declined gradually
afterward to the baseline level after 4 to 24 h.
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It is being observed that some surgeons prefer to do reverse shoulder replacement rather than attempting a rotator cuff repair in the elderly patients giving justification that the results of rotator cuff repair are not 100%.
Is it scientifically correct to offer reverse shoulder arthroplasty for roator cuff tear?
It dipendes on the quality of soft tissue,bone and the possibility to make a transfer.
The age over 80 in active patient is a good indication for rsa..
Ciao
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In Markov model of the system, I am using minimum repair, when the system is in absorbing state again I applied minimum repair to bring it back to the working condition. But When I tried to find the MTBF of the system it becomes infinity why is this is happening?
Without the model, it is not really possible to answer to your question.
Anyway:
- If you allow to jump out of the absorbing state, this is no longer an absorbing state.
- If you really have an absorbing state (without repair), this models the occurrence of the first system failure. As it is not repaired the MTTR goes to infinity and the MTBF = MTTF+MTTR goes also to infinity. Therefore you result is "normal" because in this case the concept of MTBF is meaningless.
Beware of the definitions which are often mixed up:
- MTTF: mean time to failure
- MTTR: mean time to restore
- MTBF: mean time between failures = MTTF+MTTR
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We have a Mettler AX1005 Mass Comparator giving an Error 4 code.This code due to enteral fault. I'm wondering if anybody has more infor. and if this is repairable.
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How can I do a modeling of emergency in future of the network
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Carbonation and chlorides attacks induce the corrosion of rebars in concrete. Fig 1 shows the rebars corrosion, cracking, and spalling of concrete cover. The repair methods commonly include mortars (cementitious & resin) and coatings (cementitious & polymer modified). What are other emerging repair trends of concrete corrosion deterioration?
You can find summary for different techniques in the following research
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What are the methods available to predict the optimal preventive maintenance interval for a repairable system?
Reliability Centered Maintenance is the best mehtod from my experience. You need to have a good FMEA and FMECA (system oriented) to support you, plus good a weibul. This is normally a result form some data gathering or database analysis, much better with operational and failure data to back it up, but you could do with technical experts in case of lack of data as a star point, and later implement the operational and failure database - also look at FRACAS.
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If rubber product get bloomed, can we repair it or clean it and ensure that it will not happen again?
Basic conditions are formulation and temperature within service or process . Feel free to contact me for details.
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Running 2-way ANOVA in R, I got into this error message:
"Column name `std.error` must not be duplicated."
"Use .name_repair to specify repair"
I checked the "aov model" and there is no std.error, nor duplicated columns. Also, it's tough to understand the output when I run: run rlang::last_error or trace()
I've tried everything, but I can't fix it!
I never had that one but I already saw it in someone else's code, I think it was due to a problem in the 'rstatix' package.
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Remarks about possible sources of nerve cells origins:
1. Repairing nad creating neural cell systems in human body.
2. It may be possible to learn from embryonic cell research and understanding the genetic material - load.
3. The limits of cell sorting and development must of course be addressed in a negative direction (cancer). Possible genetic mutation or P53 protein error should be considered.
4. It may also be possible to learn from the above research regarding the formation of cancer and / or mutations, and vice versa.
5. There is also room to consider understanding about tissue regrowth and repair (possibly more relevant to body organs).
Interesting
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As both a researcher and a patient, I am wondering what the best approach for a hernia repair in a patient who has had 6 inguinal hernia repairs, 4 on left side and 2 on right. The next surgery will be the 7th repair for a left inguinal hernia occuring near the location of the plug placed during the last repair.
Pt history: male 36 years of age with hx of smoking, overweight but not obese, General health his good. Hernia's have been reoccurring since 18 mos with hx of strangulation and has had an umbilical hernia since birth that has caused no pain or issues.
The newest hernia is small, but very painful for the pt who has been refused pain management by primary care and pain specialist.
Should laparoscopic repair be done for the 7th or should no repair take place until it is medically relevant?
Besides my previous answer, I think it would a good method the placing of a powerful and unique netting (mesh) to reinforce the abdominal wall.
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I am a doctoral student, I work on repair materials for concrete structures.
Dear Karim
I don't think what you wish to do is possible; that is to obtain the bond strength without destructive testing. There is no way to know how the old material behaves.
Since it is a repairer job then every case is different. You need to set up lab testing by trying to emulate the actual cases in the lab condition.
You can perform tests in the lab, but again you must carry on loading until everything falls apart. The only consolation is testing done using samples, not the real structure. However, you have the problem of correlating the test result to the behaviour of the actual structure.
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All of the recent peer reviewed Medical research articles published in prestigious medical journals, that I have read recently (since COVID-19 at least ) that were written using these massive databases, often national and even international collections of data, I consider to be invalid. The data gathered , is for the most part missing, unqualified, unusable, etc. The methodology is completely inadequate, the confounding variables rampant. Despite this and more, the authors are making conclusions and recommendations based upon these invalid results. If there is a statistical analysis, they even use the method called "bootstrapping" statistics to achieve significance, when there is none. The old saying: "G...... in, g...... out" may be aptly applied here. Please, repair your system before you publish any further. I had always thought that these massive computerized data bases would lead to great discoveries, etc., and that they would eliminate the old ways of doing research. But this is not to be. The data input needs to be much better in quality to get good results and valid conclusions. Unfortunately, publications were submitted too soon. Retraction of many of these massive computerized data based articles by prominent Medical Journals recently, exemplifies my discussion. Please take your time to repair your system so the input data is good, useful, and valid. Obviously, much more research is required. Thank you for your support. June 26, 2020. Gary Ordog, MD.
This is probably not the way to deal with this problem, but i assume someone became desperate:
World expert in scientific misconduct faces legal action for challenging integrity of hydroxychloroquine study
Australian and international scientists publish open letter defending Dr Elisabeth Bik and calling for science whistleblowers to be protected
Dutch expert in identifying scientific misconduct and error Dr Elisabeth Bik has been threatened with legal action for challenging the findings of a study promoting the drug hydroxychloroquine to treat Covid-19. Photograph: George Frey/AFP/Getty Images📷
Melissa Davey
@MelissaLDavey
Sat 22 May 2021 06.01 BST
A world-renowned Dutch expert in identifying scientific misconduct and error, Dr Elisabeth Bik, has been threatened with legal action for questioning the integrity of a study promoting the drug hydroxychloroquine to treat Covid-19.
The case, filed with the French state prosecutor by controversial infectious diseases physician Dr Didier Raoult, has prompted hundreds of scientists from across the world to publish an open letter calling for science whistleblowers to be protected.
In March 2020, Bik published a blog post analysing a paper led by Raoult. His paper claimed the antimalarial drug hydroxychloroquine was effective in treating Covid infections, especially when given in combination with an antibiotic.
📷French professor faces disciplinary case over hydroxychloroquine claimsRead more
Bik raised questions about the paper’s methodology, including that the researchers had failed to control for confounding factors. In strong clinical trials, the control group (who are given a placebo) and the treatment group (who are given the drug) should be as similar as possible so scientists can be confident any effects are from the medication alone.
Bik pointed out that patients should be of similar age and gender ratio, be equally sick at the start of treatment, and analysed in the same way, with the only difference being whether they received treatment or not. She said the treatment and placebo groups in Raoult’s study differed in important ways that could have affected the results.
Six patients enrolled in the treatment group at the beginning of the study were not accounted for by the end, missing from the data.
“What happened to the other six treated patients?” Bik said.
“Why did they drop out of the study? Three of them were transferred to the intensive care unit, presumably because they got sicker, and one died. It seems a bit strange to leave these four patients who got worse or who died out of the study, just on the basis that they stopped taking the medication … which is pretty difficult once the patient is dead.”
Dr Didier Raoult has threatened Dr Elisabeth Bik with legal action. Photograph: Daniel Cole/AP📷
Despite questions being raised by Bik and other scientists, the then US president Donald Trump promoted Raoult’s paper and the use of hydroxychloroquine for Covid, helping the French doctor gain attention.
In July, France’s Infectious Diseases Society filed a complaint against Raoult that said it was unethical to promote hydroxychloroquine during a pandemic given there was little evidence it worked against Covid. The International Journal of Antimicrobial Agents raised similar concerns.
Evidence from multiple strong and properly conducted studies has since found hydroxychloroquine has little to no impact on illness, hospitalisation, or death from Covid.
Bik, who is hired as a consultant by scientific institutions worldwide to analyse data and identify research misconduct, went on to identify image duplication and potential ethical issues in 62 published articles from Raoult and his institute.
Raoult and his supporters have relentlessly attacked Bik since, calling her a “nutcase” and a “failed researcher” on Twitter and in media interviews, and publishing her contact details online. This is despite Bik also exposing errors in papers that found hydroxychloroquine provided no benefit to Covid patients.
Raoult’s colleague, Prof Eric Chabriere, revealed on Twitter that he and Raoult have filed a complaint against Bik and Boris Barbour, who helps run a not-for-profit website called PubPeer which allows scientists to analyse and provide feedback on each other’s work.
The legal complaint alleges harassment over Bik exposing data errors on PubPeer, and extortion because she has a Patreon account where people can donate to her work. She has responded to Raoult’s calls on Twitter to declare who is funding her by sharing links to her Patreon.
Lonni Besançon, a French postdoctoral research fellow at Monash University in Australia, said he had also received multiple death threats from Raoult’s supporters after raising concerns about Raoult’s work.
“But the attacks I get as a white man are a fraction of what women researchers and researchers of colour get,” he said.
He and other international scientists have published an open letter calling for the attacks on Bik to stop and for the scientific community to support her and other whistleblowers. As of Saturday it had more than 500 signatories.
“Scholarly criticism, particularly on issues of research integrity, is fraught with challenges,” the letter said.
“This makes it particularly important to maintain focus on addressing such critique with scientific evidence and not attacks on people’s appearance, character, or person.”
📷Surgisphere: mass audit of papers linked to firm behind hydroxychloroquine Lancet study scandal
Bik was recognised internationally for conducting work “instrumental to ethical, sound, and reproducible research, but it also introduces her to personal risk as a whistleblower”.
“For several months, Raoult and some members of his institute have responded by insulting her on national television, disclosing her personal address on social media, and threatening legal action for harassment and defamation.
“Prof Raoult and his team’s behaviour toward Dr Bik and others have been pointed out by many international media outlets … This strategy of harassment and threats creates a chilling effect for whistleblowers and for scholarly criticism more generally.”
Guardian Australia has contacted Raoult for comment.
Besançon said institutions and policymakers needed to act to protect whistleblowers like Bik, saying the threats against her had become “ridiculous”.
“We also want citizens and scientists who might try to sue whistleblowers to understand that it’s not a legal concern but a scientific one,” he said. “Dr Bik is an amazing scientist and we want to make sure that no dangerous precedent is set with the current legal situation around her.”
A separate Change.org petition was also launched in support of Bik by French citizen science movement #Citizen4Science, and also had more than 500 signatures.
“The international scientific community and many of our doctors, pharmacists, caregivers, scientists and informed fellow citizens are worried and disillusioned to note that our country is allowing disinformation, cyberstalking and legal proceedings to flourish against the carriers of consensual scientific speech,” the petition, led by #Citizen4Science president Dr Fabienne Pinson, says. “The perpetrators are not even worried or simply called to order.”
Pinson told Guardian Australia the petition was “an active call to French authorities to intervene” and stop harassment of scientists and misinformation.
(My conclusion: Interesting, by speaking up about scientific misconduct you become threatened with a lawsuit. Why not just defend your research, why make the threats which in itself appears that you are fraudulent for having to do so. This is not the scientific method.)
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Our facility is currently using Millipore Intergral10 for filtered water. We are very happy with the system and have had it for many years. The downside is the service contracts, each year it is very expensive for a 1X a year PM and maybe a once a year repair... if that.
The cost for a yearly contract is around 5-6,000 dollars.
My question is has anyone used the Elga Veolia water purification in their lab and if so please let me know your opinion.
Here are the links of the water purification systems based on lab scale and am sure you will love these and I have personally tried using products from here for my college laboratory and also at one of the project and both are absolutely marvelous
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Please explain why do we take a repair rate equal to 1 when my failure rate data is in per/month for calculating the availability of the system
please explain it little more, I could not understand
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What is the current premier assay for monitoring DNA damage, break formation and/or repair in cell culture? Is it immunofluorescence based (e.g. fish, antibody). Or are there commercial assays that exist. Any insight would be greatly appreciated.
Single cell gel electrophoresis (SCGE; comet assay) is a widely-used method due to its technical simplicity and sensitivity.
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I am running an experimental program of a doctoral thesis, I need this information to be able to complete my study
You can use a concrete surface profilometer
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Goal: Hi My project is about the feasibility of using pre-prepared hot and cold asphalt to repair the pavement around the manholes. I ask my dear researcher to help me if I have experience or information in this field?
There is wide range of epoxies paste that handles such problem sufficiently. Make a parametric study with this!
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Hello,
Here is my experiment:
I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making it a circular piece of dsDNA. I then use a qiagen kit to prep the plasmids from the cells directly. I now have a tube with a pool of "repaired" circular plasmids all of which are unique repair events. I would like to use asymmetric PCR to amplify this pool so that I could visualize the relative size of the repaired plasmids on an agarose gel. Some repair events cause large deletions in the plasmid, so the pool of repaired plasmids will contain circular DNA ranging in size from 4kb to 12kb. I want to use the Phusion polymerase with only one primer in the reaction to amplify the plasmids so I can compare the size of deletions that occurred in my WT cells vs my KO cell line. (we plan to do Next Gen Sequencing on the pool, but it is SO EXPENSIVE that we are looking for a more cost effective "dirtier" method to complement it)
My questions:
Once the Phusion polymerase copies the entire circular plasmid and ends up at the forward primer again, will it just keep on going around and around amplifying the circle or will it terminate once it reaches the bound primer?
Should I digest my plasmid pool before aPCR?
cheers,
Valerie
Interesting
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I am on a quest to solve how a cell repairs itself through encoding-decoding of proteins. Is there any link to genetic algorithms to solve age old questions such as aging and how we heal?
Hi,
I suggest you to see this link i hope it's in the topic.
Best regards
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I am reviewing and gathering information on “shipbuilding operations management”. This research is done in the following three sections.
1. Ship design
2. Manufacturing and construction (Shipbuilding)
3. Ship repairs and maintenance
We have faced a great challenge as follows, and I ask for your help in this field. Sending a file, article, book, or any information in any language will help us a lot.
I seek to classify old and new methods and approaches in the above three areas (Ship design, manufacturing and construction, and ship repairs and maintenance). This information can be separate or in the form of tables and figures.
best regards
Armin
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Dear,
We developed a microcarrier that was pre-seeded hUC-MSCs to repair a rat bone defect. However, we didn't use GFP or Luciferin to track cell survival. If there's any way to identify the human-derived cells from the rat cells in the histological section? Much appreciation for your reply.
Simply use anti-human secondary antibodies, since you have human cells in the rat tissue i.e. https://www.thermofisher.com/antibody/secondary/query/anti-human/
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is anyone know research or publications about the repair of mitochondria injury?
few articles
Mitochondrial DNA damage and repair during ischemia–reperfusion injury of the heart
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How can stem cells repair damaged cells?
Could anyone provide papers show the therapeutic potential of stem cells in vitro (on the disease model)?
The therapeutic effect of stem cells (e.g., BM-MSC) is mainly due to the secretory activity through the expression of cell-specific proteins - growth factors, interleukins, etc. So you can apply even the condition medium without cells to obtain a therapeutic effect.
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Kindly I.am going to search on lameness and limb function after fractures repair in rabbits ,and for that I. am going to estimate an (limb gait score) in which evaluation of the fractures repair post operation done by the clinical observation of animals lameness and the character of the of the animals attitude during fractures healing processing .
With my respect and thankful if I receive an scientific paper for that.
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What are the new ways to repair bridges?
What are the new ways to repair pavement bridges?
What are the new methods of repairing paved concrete or metal bridges or railways?
This Manual might be useful to you -Concrete Bridge Protection, Repair, and Rehabilitation Relative to Reinforcement Corrosion.
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In indirect contact condensers, which are used for power plants with non-dry cooling towers. The quality of circulating water is lower than that of dry towers. Due to the open cycle of the circulating water, the condensers are controlled in overhauls. The formation of hard deposits on the inner wall of the condenser reduces the volume of circulating water in the condenser and copper pipes.
Is there an effective way to control these sediments?
regular water lancing .. jet cleaning ... improving feed water chemistry .. many many factors besides these measures
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Ecological services provided reclamation of wasteland is an important issue to get more benefits. The benefits with respect to oxygen emission for human beings along with other parameters viz, soil repair, soil erosion loss, nutrient inputs, visit by wild animals and so on.
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Recently, our 5000 rpm centrifuge (Chinese manufacturer) started slowing down much slower than before. Typically it took 2-3 min to go from 5000 rpm to stop before, now it takes ca. 10 min.
The noise and vibrations of the centrifuge have not changed.
questions:
what could be wrong with it?
how can that be repaired easily?
Thanks a lot for your help.
I have never personally used one, but some centrifuges (and other rotating power equipment) use an electromagnetic brake to decelerate. If there is a circuit that converts the rotational kinetic energy into current (i.e. has a 'generator' function) and then shunts the current to the resistor, the process of electrical generation will impose a drag force on the rotating component. If the circuit becomes open, no electrical current will flow through the resistive (dissipative) element and the elevated drag of will no longer be generated.
Unplug the machine and let it sit for a few minutes to discharge any capacitance in the controls or motor drive circuitry. Then examine the internal circuitry for a large or high-power rating resistor(s) or 'shunt' physically isolated from other electronic components for elevated temperature. Use a volt meter to check for resistance across the shunt resistor and of that circuit in general. If resistance across the shunt is off-the-scale high, the resistor could be failed. Also, a physically-large resistor with burn marks on or around it is another clue of over-temp failure, as could be a burnt odor coming from that component. Replacing a shunt resistor must be done by specifying a power dissipation significantly greater than the expected shunt power demand, to avoid over-temp failure.
If the machine was designed with too low a power rating for that resistor, replacing it with one of the same power rating will likely result in repeat failure. This becomes a question of how well you trust the manufacturer or the supply vendor selling the product.
If any other parts look or smell burned, those would also be good hints as to what the failure could be.
Tinkering with the internals of lab gear is a good way to void any warranty if one exists, so be advised and don't go probing around with any metal or conducting objects while the thing is open, don't leave it open, and don't let anyone else near it while it is open. A centrifuge is a finely balanced instrument and should be treated with care, but many simple failures can be fixed fairly easily or simply after locating and diagnosing the trouble. Unfortunately a burned-out resistor or capacitor may no longer have any readable label or spec value remaining, and determining the spec for the replacement is important for proper and safe function going forward. Good luck and give us a report back. -TH
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Does anybody has any idea about any study or protocol doing furcal repair in rats molar. i have seen many studies but for pulp capping, pulpotomy, intraosseous or subcutaneous implantation of any material to be tested. but why not furcation area. please clarify
Periradicular inflammatory response, bone resorption, and cementum repair after sealing of furcation perforation with mineral trioxide aggregate (MTA Angelus™) or Biodentine™
Magda de Sousa Reis, Roberta Kochenborger Scarparo, […]José Antônio Poli de Figueiredo
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3 Citations
Metrics
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A very thin crack was produced in the alumina tube of our tube furnace possibly due to some thermal shock. I want to know is there any solution to fill that crack.
Generally, two types of crack might occur on the alumina tube. The cracks are fairly common and are caused by the stresses generated by heat. The cracks are fairly common and are caused by the stresses generated by heat.
1. Radial crack at or near the seal to the alpha ring.
2. Longitudinal crack running along part of the length of the tube.
It is possible to fix a leak in a ceramic pipe yourself if you do not want to retrofit or replace the pipe yet. This method can be used whether a pipe is simply cracked or has sprung a leak.
You can fix the leak or crack by simply installing inflatable pipe tubing. Look for a piece of tubing that is large enough to cover the leak or the crack and insert it in the pipe. Push it until it covers the leak or the crack. The tubing will inflate once you pull the cord. The tubing will automatically stop inflating once it touches the pipe and should prevent the water from leaking. Keep in mind that this is a temporary fix. This is an easy way to fix a pipe that is leaking or that is cracked but you will eventually need to get a plumber to replace the pipe.
Vacuum the dust from the damaged area. Remove all the contaminant. Otherwise it will melt and embed deeper. Smooth the surface mix with filler and with a small putty knife. Allow it to dry before firing the tube. You can repair using fller such as Sheffield Bronze 1454 Porcelain Filler/Repair/ Fixture Fix PF-12 Fill-A-Fix Porcelain Filler/ Minwax 21600000 High-Performance Wood Filler etc.
Refer site:
Ashish
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I am working on a resource allocation problem using an SPEA 2 evolutionary algorithm. The problem involves a decision variable where each variable has a different domain e.g. Ei < di where Ei is the allocation to a user and di is individual demands. The problem involves a linear constraint such that sum(Ei) = total resource. The probability of the creation of feasible off-springs after crossover and mutation operators is extremely low. So, we need a repair operator for this purpose. I need guidance for the selection of suitable repair operators and how should I apply that, I mean should we repair all solutions or some percentage. I would appreciate the guidance, comments, or any literature reference.
Saifullah Khalid What is the objective function? I do not find it in your description That would otherwise be very handy to have. Also, you need to restrict E_i to be non-negative.
I can - with almost certainty - say that this problem is a standard resource allocation problem, for which there are solvers that guarantee to provide an optimal solution, within a second. (My papers on it describe a great variety of models and approaches.) Hence, skip the metaheuristic, please - I do not think it is going to provide an optimum, while using the simple tools described in my papers will always yield optimal solutions.
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I am converting fragility curve in to vulnerability curve. How to develop a smooth curve by adding repair to replacement cost ratios?
This report from GEM (https://storage.globalquakemodel.org/media/publication/GEM-GC-VLM-AVALMGuidelines-201412v01.pdf) provides both guidelines and examples on how to derive vulnerability curves from fragility curves.
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In other words, for "unavailability" calculation, is the use of the known "exp" equation, sufficient?
Is it right to consider the degradation state for such a component?
Refer to Factory Physics book by Wallace Hopp. The model presented there can be simplified by treating repair time to be constant.
Alternatively, you may try integrating (1-Availability)*AverageServiceTime in the Coefficient of Variation corresponding to Average Service Time.
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What are the reasons for the failure of the indicated pier of the bridge? What is the expected type of load that led to this damage? Design errors or the approved specifications did not cover this type of failure is avoided? Was the failure less severe if two circular piers were used instead?what is the possible method to be adopted to repair this damage?
You can find good information in the following book: https://www.amazon.com/Analysis-Design-Cable-Stayed-Bridge/dp/3847303899
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During repairing or removal of bituminous roads large fragments of bituminous road are generated as waste. How to use them? Can we extract bitumen and aggregates from them.
You use can use the RAP in hot mix asphalt plants production either in smalll proportions (say upto 15% of total mix weight) in what we call "cold process" or at higher proportions (say 40% and higher) in "hot process". For the hot mix process you will need a paralel drum (in batch plants). A very good sourc for information is the DIRECT - MAT project deliverables:
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In an article, I read that "In all mammalian cells, DSBs that occur throughout the cell cycle are repaired predominantly by the non-homologous DNA end joining (NHEJ) pathway."
As we know NHEJ is an error-prone repair mechanism, so why cell choose this pathway rather than Homologous recombination pathway which is error-free?
To answer briefly: Homologous recombination occurs in S-G2 phase when the template is present. It is said that it would avoid errors. NHEJ ligates DNA breaks which are more or less next to each other (DNA never flies freely!!). This process could be error prone depending on the involvement of other DNA repair functions and the kind of break (blunt , not blunt). It is said that NHEJ is a source of DNA sequence evolution.
Hope it helps.
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Active debate over the ethical and legal status of robots and AI
Robotics and AI are a fast-developing field, and we all urge policymakers to recognize not only the potential of robotics, but also the risks and challenges relating to “human safety, privacy, integrity, dignity, autonomy and data ownership”.
All of us should consider questions of liability and the potential legal status of robots. The more autonomous robots are, the less they can be considered tools in the hands of other actors (such as the manufacturer, the owner, the user, etc.).
• Thus, can the machine be held responsible for its acts or omissions?
• And should robots possess a legal status? (Notes: current legal categories are “natural persons”, “legal persons”, “animals” or “objects” – but does this mean that a new category should be created, with its own specific features and implications as regards the attribution of rights and duties, including liability for damage?)
• Should we give robots the right to kill humans?
• Should we give robots the right to kill theirselves?
• Must Inventors Be Humans?
New EU rules for the ethical and legal status of robots and AI
On 16 February, 2017, the European Parliament’s Plenary Session adopted the JURI report on civil rules on robotics (European Parliament resolution of 16 February 2017 with recommendations to the Commission on Civil Law Rules on Robotics (2015/2103(INL))) with recommendations to the EU Commission on Civil Law Rules on Robotics. The resolution was based on an in depth report (DRAFT REPORT with recommendations to the Commission on Civil Law Rules on Robotics (2015/2103(INL)) that delineates core challenges of the evolving field of robotics, including the ethical and legal issues, and provides recommendations for the development of the field in Europe.
EU scientists denied robots legal personhood
As for 4 May, 2018, in an open letter, 156 artificial intelligence experts from 14 European countries have rejected the European Parliament’s recommendation that robots should have legal status as electronic persons. This would make robots responsible for repairing any damage they might cause.
References:
Should robots have their own legal status?
I agree with 156 artificial intelligence experts who signed an open letter on the 4th May, 2018 REJECTING the European Parliament’s recommendation that robots should have legal status as electronic persons.
My primary reason is simply due to legal accountability, law and order.
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If I have more number of foci, does it represent nuclear damage or nuclear repair? Some cells show pan nuclear staining, does it mean the cell underwent apoptosis?
I am new to this area. Any help would be appreciated.
This paper is an excellent summary of DNA damage foci by Kai Rothkamm https://www.ncbi.nlm.nih.gov/pubmed/?term=foci%2C+meaning+and+significance%2C+rothkamm
Steve
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There is a crane girder of a built-up I-section with span 12 m simply supported on two brackets. After 15 years of operation of double girder overhead crane, the girder is currently deflected downwards up to 3 cm at some points while the allowable deflection is L/800 which is 1.5 cm. What is the best action for this girder?
it depends on which cost less repairing or replacement , after 15 working years i suggest the replacement of damaged beam ( where the fatigue took place ) is more safe .
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I am working on a paper in Conversation Analysis, titled “Toddlers asking Amazon Echo Alexa to play the song baby shark”
When analyzing the data i faced this issue of (when toddlers ask Alexa to play the song, and Alexa doesn’t respond to them, or doesn’t process their words correctly; due to their bad articulation,, what they do is repeat themselves again with the same exact words)
Is that considered repair or confirmation.
Videos‘ links are below for better understanding.
If they repeat themselves only or more often after Alexa's non- or wrong response, I would consider it an attempted repair. The first video clip shows a variety of both repairs and confirmations IMO. The girl frequently says 'Yeah' as a confirmation, but almost all of what I hear and see in both videos are attempted repairs. Some are way more clear than others, as when volume or annunciation changes. Although toddlers are not bad at inferring why a conversational partner doesn't respond appropriately, they are far from skilled & not infer that the partner doesn't process the pronunciation appropriately.
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do someone has any resource about repairing leakage in as cast pipe and fittings?for example welding, powder or polymer repairing agents.
Blowhole is a cavities defect always located in the cope part of the mould in poorly vented pockets and undercuts. Blowhole is divided into pinhole and subsurface blowhole type. Pinhole is very tiny hole while subsurface blowhole only can be seen after machining. Reason, gases entrapped by solidifying metal on the surface of the casting, which results in a rounded or oval blowhole as a cavity associated with slag’s or oxides. Sand inclusion, sand burning, cold lap or cold shut, misrun, gas porosity, mismatch defects, distortion , thermal defects can occur during welding. Possible causes are due to Inadequate core venting, excessive release of gas from core, excessive moisture absorption by the cores, low gas permeability of the core sand, moisture content of sand too high, or water released too quickly, gas permeability of the sand too low sand temperature if too high, bentonite content if too high and too much gas released from lustrous carbon producer. by reducing this all causes blowhole in ductile iron welding can be stopped.
Remedies
Improve core venting, provide venting channels, ensure core prints are free of dressing, Reduce amounts of gas, use slow-reacting binder and reduce quantity of binder. It is better to use a coarser sand if necessary (soft sand permeability problem), apply dressing to cores, thus slowing down the rate of heating and reducing gas pressure, dry out cores and store dry, thus reducing absorption of water and reducing gas pressure, reduce moisture content of sand, Improve conditioning of the sand, reduce bentonite and carbon carrier content, reduce sand temperature, installing a sand cooler if necessary etc.
Ashish
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I have read that turmeric inhibits telomerase. I have also heard telomerase helps repair telomeres. Does this mean turmeric stops telomeres repairing or have I heard wrong?
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Most abdominal solid organ trauma (when the patient is stable) can be managed non-operatively (e.g. liver, kidney, spleen). However, it seems as though many complications of testicular trauma require surgical repair (e.g. testicular rupture, any penetrating injury, etc.). What might be the reason for this? Does it have anything to do with easy access (i.e. testicles are quite superficial)?
Surgeon decides if there is need to do open or laproscopic surgery. If there is fine surgery to be done like microsurgery, probably opening scrotum skin will be an option. It will be painful for few days but that can be controlled with medicine.
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some of the Chinese manufacturers use special powder for repairing leakage of pipe and fittings after casting. they heating the pipe wall and then add this powder on the surface of leakage area.
Ni-Si-B alloy is not likely regarding the process described.
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How to fix the Error: The mesh file exporter could not resolve cyclic dependencies in overlapping contact regions. Please try to Repair Overlapping Contact Regions.
Contact regions are said to be "overlapping" when the same entity is a member of more than one contact region or when multiple contact regions share the same geometry. Ansys fluent does not support overlapping contact regions.
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I'm working on inserting a 41bp sequence into a mouse medep cell using hdr, i'm wondering about the arm length of repair template.
I've read many guides, some say the arm can be 30-50bp, and the efficiency will decrease if the arm is longer. But some say it's better to use 50-80bp.
I wish i can get some advices. Thank you!
Which kind of repair template do you plan to use? For inserting 41 nucleotides, I think it'd better use long repair template. Short single stranded donor oligonucleotides can be used for small insertion or modification.
For long repair template (donor template plasmid, PCR product, single stranded template or AAV), the length of homology arms depends on how specific your homology sequence is. If the homology sequence is unique, you can use a short arm. We normally use 400-2000bp length for each arm, depending on sequences. Other also reported successful integration with the length of 100-300bp in T cells.
Good luck!
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Robocasting, scaffolds, bone cement
Ali Taha Saleh kindly go through this link
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I would like to explore the possible topics to write a thesis regarding the Application of AR in Service Industry (in car repair workshops) any leads would be appreciated
Using Augmented Reality in Management of Car Service Check list
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Hi, I tried 2 conditions on two different samples of different concentrations, and one sample gives an ultra concentration, I have an Idea of whats going on here, but I want more experimented opinions.
starting concentrations
1_ 1,08 ng/uL
2_ 2,57 ng/uL
I wanted to try if the ligation at Rt and at 20ºC makes some diference (20 minutes for all)
So I divided the samples taking 5uL each and diluting it to 5ng/uL with Elution Buffer, making 4 tubes, 2 tubes for sample so It ended up like this:
1.1
1.2
2.1
2.2
5ng/uL each
Then I went to end repair and A tailing, no problem at all.
On the adapter ligation I realized that on my tube of choice there was no volume for all samples (28nM final concentration) so I thaw a new adapter before using all the first one on 1.1, so I put the new adapter on 1.2, 2.1, 2.2.
1.1 and 2.1 went for adapter ligation for 20 minutes at Rt
1.2 and 2.2 went for adapter ligation on thermbloc at 20ºc for 20 minutes.
I´ve done the post adapter ligation cleanup with agencourt AMPure XP beads with no problem, I eluted the DNA with 20uL of elution buffer.
Then the samples went for library amplification for 12 cycles, and then I eluted the product again with the QIAquick MinElute kit columns & buffers.
Final concentrations goes like this (2uL samples on Qbit)
1.1- 3,32 ng/uL
1.2- 2,93 ng/uL
2.1- 6,96 ng/uL
2.2- 8.3 ng/uL
Is it possible that I didn´t resuspended well the new adapter after thawing it and after puting it in 1.2 so It was more concentrated and amplified more?
any other idea?
thank you so much!
You should have more DNA post amplification.
Sample 2 also started with double quantity, so it make sense that amplified sample 2 would have higher DNA (remember amplification is exponential).
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I'm actually trying to transform two plasmids into yeast. 1. pRS416 with URA3 gene, 2.pRS413 with His3 gene. At first, i have transformed pRS416 into yeast that worked (got colonies on -URA plate). Then i have used these colonies, grew them using -URA media and tried to transform intact/linearized pRS413 using the same procedure. And plated them on -URA-His plates. Now my cells had (probably!) intact pRS416 and intact/linearized pRS413. After 3 days, I have observed colonies with intactpRS416+ intact pRS413 but i didn't see any colony on intact pRS416+linearized pRS 413.
PS: we know that plasmid pRS416 can produce a protein that will repair linearized pRS413 to make it intact again.
Amounts: plasmid=50ng, SS-DNA=100ug.
Anyone has any suggestion to transform linearized pRS413 into yeast cells? Thanks in advance!
@Dr Faris AbachI: we have solved our problem by stably integrating our expected gene into the yeast genome. So now we need to transform only one plasmid instead of two (as the expression of this integrated gene will repair the linearized plasmid). NB. We have used 250ng of linearized plasmid but we saw that more DNA will produce more colony!!
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I have conducted a field survey among 100 residential houses asking for the cost of repairs, the floor area, number of floors, age of structure, and I want to relate the cost to these parameters. What function would be best to fit the data?
Dear Christian,
let me suggest you our recent research on the topic of the structural vulnerability of masonry buildings to floods where the role of velocity on the stability of the building is explicitely discussed.
Luca
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