Questions related to Repair
Research studies including randomized controlled trials often have a time-to-event outcome as the primary outcome of interest, although competing events can precede the event of interest and thus may prevent the primary outcome from occurring - for example mortality may prevent observing cancer recurrence or may preclude need for reoperation in patients who undergo surgical repair of heart valves. Researchers often use Kaplan-Meier survival curves or the Cox proportional hazards regression model to estimate survival in the presence of censoring. These models can provide biased estimates (usually upward) of the incidence of the primary outcome over time and therefore other models which address competing risks, such as the Fine-Gray subdistribution hazards model, may be more suitable for estimating the absolute incidence of the primary outcome as well as the relative effect of treatment on the cumulative incidence function (CIF). My question is whether the Nelson-Aalen estimator is a reasonable option for estimating the hazard function and the cumulative incidence of the outcome of interest in the scenario of competing risks and if so, why is this a preferred approach over the Kaplan-Meier estimator?
In a recent lecture during the construction of a temple in our region, it was shown that one of the rock got cracked during the excavation done for the foundation work and the rock is being repaired by stone stitching (crack stitching) technique and wanted to know more about the same.
How can I measure (or calculate) the below fluency?
Could anyone explain to me with an example of two people?
When I read the paper, it does not explain how I can calculate it exactly?
Thanks a lot
(1) Speed fluency
A. Articulation rate:
mean number of syllables per minute divided by mean amount of phonation time (excluding pauses)
B. Speech rate : mean number of syllables per minute divided by total time (including pauses)
(2) Breakdown Fluency
A. Mean length of pauses per 60 seconds
B. Mean number of pauses per 60 seconds (clause-internal versus clause-external)
(3) Repair fluency
A. Repair measures : mean number of partial or compete repetitions, hesitations, false starts and reformulations
B. Mean number of filled pauses (e.g., em and er)
(4) Dialogue only measure
A. Number of turns
I have four female carolina sphinx moths as pets and one of them was unable to fuse her proboscis after emergence. Is it possible to help her repair her proboscis to enable her to continue to drink?
Can anyone help me with finding a manual for the Zeiss Jenalumar microscope.
We found a 1983 model in the stores and as far as we can tell, the optics are far better than anything we could afford to buy today. Unfortunately, the instructions manual is missing and Zeiss haven’t answered my request for a new one. I managed to find the repair manual on the internet, but not the instructions for use.
Recently, the XPS instrument in our lab underwent repair and maintenance. The difference that I can find now is that I am unable to get a clear focus of my sample (when it is inside the analysis chamber) as I used to get earlier. This is degrading the quality of my plots with no clear peaks to be seen. Any troubleshooting suggestions would be highly appreciated!
Is measuring Bst Polymerase activity (by comparing it with NEB Bst Polymerase) using SYBR and FLUOstar Omega machine (because our RT-PCR machine has been broken and I'm not sure when it will get repaired).
FLUOstar Omega is a microplate reader that have function for incubation with temperature up to 65 degree C (LAMP reaction need 60).
Here is the list of detection modes this thing have:
Fluorescence intensity - including FRET
Luminescence (fl ash and glow) - including BRET
Time-resolved fl uorescence - including TR-FRET
does anyone have experience like this?
any opinion/suggestion would help alot.
Thanks in advance! :D
Our lab purchased the Spectramax ID3 and have encountered an error in the built in computer due to an issue connecting to the hard drive. Has anyone had experience with this and know of a resolution or the cost of repair?
Nowadays mass spectrometry instrument vender is not providing repair or service manual. Third party service provider is not going to teach you how to repair the instrument either. Communicate with other user is very helpful.
Dear limnologists - marine scientists
I am looking for a sampler that can collect integrated water samples in the 0-20m depth range.
In our lab we have an old, mechanical Schröder bottle which unfortunately stopped working and may no longer be repaired. We'd prefer to replace it with an equally simple, mechanical device, but I'm having a hard time finding one.
Do you have any suggestions?
Thank you in advance,
A 23 year old female sexually active was diagnosed with MRKH has associated RVF. MRI shows incomplete vestigial uterus and absent vagina. Examination reveals RVF. Bowel (ileal) neovagina construction and RVF repair done at the same time. Are there any alternative approachs?
We are designing a strategy to make CRISPR knock-in intestinal organoids using Lentiviral delivery (as other transfection method are very inefficient in intestinal organoids). For designing the guide RNA, this seems straightforward, and we are planning to use the lentiCRISPRv2. However, for the repair template we are struggling to figure out which backbone would be best. The worry is that cloning our repair template with homology arms into a constitutive Lenti plasmid would result in random insertions of the homology arms and tag instead of being used as a repair template. Does anyone have experience doing CRISPR knock-ins using lentiviral delivery that might be able to give some advice? Thanks!
Is anyone familiar with DIY repair of the Typhoon (Amersham/GE/molecular dynamics) lasers canners? Can't find any hints online. Our current issue with it is a faulty shutter cable.
BTW, in case anyone is stuck with a "firmware" error in the older machine (ours is 2007 and is beyond repair), one can open the PC box (in 2007, it was a Pentium III) and connect a VGA monitor and a keyboard to the PC board, and read the error code that comes up during start up. Ours shows a shutter cable problem, which is where I am stuck now.
In the past, I have replaced faulty fans, and the fast stage drive belt. All of these "problems" were quoted by GE to be fixed for between 5K and 15K, at which point the machine has been abandoned, and I've been fixing issues with it for the last few years on case by case basis.
Recently we found in a mineral processing mine that column was damaged due to corrosion to a significant level. In this it seems base plate was also corroded. This requires almost entire replacement. Do we have any alternate way of repairing the same.
In some case the lower portion of columns was encased in concrete to prevent this type of failure, whether making lower portion as a composite column is advisable as this will change the entire behaviour.
Hello every one
my question is about in vitro exposure of blood samples (blast cells) with ionization radiation to repair DNA damage.
It is being observed that some surgeons prefer to do reverse shoulder replacement rather than attempting a rotator cuff repair in the elderly patients giving justification that the results of rotator cuff repair are not 100%.
Is it scientifically correct to offer reverse shoulder arthroplasty for roator cuff tear?
In Markov model of the system, I am using minimum repair, when the system is in absorbing state again I applied minimum repair to bring it back to the working condition. But When I tried to find the MTBF of the system it becomes infinity why is this is happening?
We have a Mettler AX1005 Mass Comparator giving an Error 4 code.This code due to enteral fault. I'm wondering if anybody has more infor. and if this is repairable.
Carbonation and chlorides attacks induce the corrosion of rebars in concrete. Fig 1 shows the rebars corrosion, cracking, and spalling of concrete cover. The repair methods commonly include mortars (cementitious & resin) and coatings (cementitious & polymer modified). What are other emerging repair trends of concrete corrosion deterioration?
Running 2-way ANOVA in R, I got into this error message:
"Column name `std.error` must not be duplicated."
"Use .name_repair to specify repair"
I checked the "aov model" and there is no std.error, nor duplicated columns. Also, it's tough to understand the output when I run: run rlang::last_error or trace()
I've tried everything, but I can't fix it!
Remarks about possible sources of nerve cells origins:
1. Repairing nad creating neural cell systems in human body.
2. It may be possible to learn from embryonic cell research and understanding the genetic material - load.
3. The limits of cell sorting and development must of course be addressed in a negative direction (cancer). Possible genetic mutation or P53 protein error should be considered.
4. It may also be possible to learn from the above research regarding the formation of cancer and / or mutations, and vice versa.
5. There is also room to consider understanding about tissue regrowth and repair (possibly more relevant to body organs).
As both a researcher and a patient, I am wondering what the best approach for a hernia repair in a patient who has had 6 inguinal hernia repairs, 4 on left side and 2 on right. The next surgery will be the 7th repair for a left inguinal hernia occuring near the location of the plug placed during the last repair.
Pt history: male 36 years of age with hx of smoking, overweight but not obese, General health his good. Hernia's have been reoccurring since 18 mos with hx of strangulation and has had an umbilical hernia since birth that has caused no pain or issues.
The newest hernia is small, but very painful for the pt who has been refused pain management by primary care and pain specialist.
Should laparoscopic repair be done for the 7th or should no repair take place until it is medically relevant?
All of the recent peer reviewed Medical research articles published in prestigious medical journals, that I have read recently (since COVID-19 at least ) that were written using these massive databases, often national and even international collections of data, I consider to be invalid. The data gathered , is for the most part missing, unqualified, unusable, etc. The methodology is completely inadequate, the confounding variables rampant. Despite this and more, the authors are making conclusions and recommendations based upon these invalid results. If there is a statistical analysis, they even use the method called "bootstrapping" statistics to achieve significance, when there is none. The old saying: "G...... in, g...... out" may be aptly applied here. Please, repair your system before you publish any further. I had always thought that these massive computerized data bases would lead to great discoveries, etc., and that they would eliminate the old ways of doing research. But this is not to be. The data input needs to be much better in quality to get good results and valid conclusions. Unfortunately, publications were submitted too soon. Retraction of many of these massive computerized data based articles by prominent Medical Journals recently, exemplifies my discussion. Please take your time to repair your system so the input data is good, useful, and valid. Obviously, much more research is required. Thank you for your support. June 26, 2020. Gary Ordog, MD.
Our facility is currently using Millipore Intergral10 for filtered water. We are very happy with the system and have had it for many years. The downside is the service contracts, each year it is very expensive for a 1X a year PM and maybe a once a year repair... if that.
The cost for a yearly contract is around 5-6,000 dollars.
My question is has anyone used the Elga Veolia water purification in their lab and if so please let me know your opinion.
Please explain why do we take a repair rate equal to 1 when my failure rate data is in per/month for calculating the availability of the system
I am running an experimental program of a doctoral thesis, I need this information to be able to complete my study
Goal: Hi My project is about the feasibility of using pre-prepared hot and cold asphalt to repair the pavement around the manholes. I ask my dear researcher to help me if I have experience or information in this field?
Here is my experiment:
I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making it a circular piece of dsDNA. I then use a qiagen kit to prep the plasmids from the cells directly. I now have a tube with a pool of "repaired" circular plasmids all of which are unique repair events. I would like to use asymmetric PCR to amplify this pool so that I could visualize the relative size of the repaired plasmids on an agarose gel. Some repair events cause large deletions in the plasmid, so the pool of repaired plasmids will contain circular DNA ranging in size from 4kb to 12kb. I want to use the Phusion polymerase with only one primer in the reaction to amplify the plasmids so I can compare the size of deletions that occurred in my WT cells vs my KO cell line. (we plan to do Next Gen Sequencing on the pool, but it is SO EXPENSIVE that we are looking for a more cost effective "dirtier" method to complement it)
Once the Phusion polymerase copies the entire circular plasmid and ends up at the forward primer again, will it just keep on going around and around amplifying the circle or will it terminate once it reaches the bound primer?
Should I digest my plasmid pool before aPCR?
thank you for your help!
I am on a quest to solve how a cell repairs itself through encoding-decoding of proteins. Is there any link to genetic algorithms to solve age old questions such as aging and how we heal?
I am reviewing and gathering information on “shipbuilding operations management”. This research is done in the following three sections.
1. Ship design
2. Manufacturing and construction (Shipbuilding)
3. Ship repairs and maintenance
We have faced a great challenge as follows, and I ask for your help in this field. Sending a file, article, book, or any information in any language will help us a lot.
I seek to classify old and new methods and approaches in the above three areas (Ship design, manufacturing and construction, and ship repairs and maintenance). This information can be separate or in the form of tables and figures.
I do not know which sources are suitable for this subject. Please help me.
We developed a microcarrier that was pre-seeded hUC-MSCs to repair a rat bone defect. However, we didn't use GFP or Luciferin to track cell survival. If there's any way to identify the human-derived cells from the rat cells in the histological section? Much appreciation for your reply.
Kindly I.am going to search on lameness and limb function after fractures repair in rabbits ,and for that I. am going to estimate an (limb gait score) in which evaluation of the fractures repair post operation done by the clinical observation of animals lameness and the character of the of the animals attitude during fractures healing processing .
With my respect and thankful if I receive an scientific paper for that.
What are the new ways to repair bridges?
What are the new ways to repair pavement bridges?
What are the new methods of repairing paved concrete or metal bridges or railways?
In indirect contact condensers, which are used for power plants with non-dry cooling towers. The quality of circulating water is lower than that of dry towers. Due to the open cycle of the circulating water, the condensers are controlled in overhauls. The formation of hard deposits on the inner wall of the condenser reduces the volume of circulating water in the condenser and copper pipes.
Is there an effective way to control these sediments?
Ecological services provided reclamation of wasteland is an important issue to get more benefits. The benefits with respect to oxygen emission for human beings along with other parameters viz, soil repair, soil erosion loss, nutrient inputs, visit by wild animals and so on.
Recently, our 5000 rpm centrifuge (Chinese manufacturer) started slowing down much slower than before. Typically it took 2-3 min to go from 5000 rpm to stop before, now it takes ca. 10 min.
The noise and vibrations of the centrifuge have not changed.
what could be wrong with it?
how can that be repaired easily?
Thanks a lot for your help.
Does anybody has any idea about any study or protocol doing furcal repair in rats molar. i have seen many studies but for pulp capping, pulpotomy, intraosseous or subcutaneous implantation of any material to be tested. but why not furcation area. please clarify
I am working on a resource allocation problem using an SPEA 2 evolutionary algorithm. The problem involves a decision variable where each variable has a different domain e.g. Ei < di where Ei is the allocation to a user and di is individual demands. The problem involves a linear constraint such that sum(Ei) = total resource. The probability of the creation of feasible off-springs after crossover and mutation operators is extremely low. So, we need a repair operator for this purpose. I need guidance for the selection of suitable repair operators and how should I apply that, I mean should we repair all solutions or some percentage. I would appreciate the guidance, comments, or any literature reference.
I am converting fragility curve in to vulnerability curve. How to develop a smooth curve by adding repair to replacement cost ratios?
In other words, for "unavailability" calculation, is the use of the known "exp" equation, sufficient?
Is it right to consider the degradation state for such a component?
What are the reasons for the failure of the indicated pier of the bridge? What is the expected type of load that led to this damage? Design errors or the approved specifications did not cover this type of failure is avoided? Was the failure less severe if two circular piers were used instead?what is the possible method to be adopted to repair this damage?
In an article, I read that "In all mammalian cells, DSBs that occur throughout the cell cycle are repaired predominantly by the non-homologous DNA end joining (NHEJ) pathway."
As we know NHEJ is an error-prone repair mechanism, so why cell choose this pathway rather than Homologous recombination pathway which is error-free?
Active debate over the ethical and legal status of robots and AI
Robotics and AI are a fast-developing field, and we all urge policymakers to recognize not only the potential of robotics, but also the risks and challenges relating to “human safety, privacy, integrity, dignity, autonomy and data ownership”.
All of us should consider questions of liability and the potential legal status of robots. The more autonomous robots are, the less they can be considered tools in the hands of other actors (such as the manufacturer, the owner, the user, etc.).
- Thus, can the machine be held responsible for its acts or omissions?
- And should robots possess a legal status? (Notes: current legal categories are “natural persons”, “legal persons”, “animals” or “objects” – but does this mean that a new category should be created, with its own specific features and implications as regards the attribution of rights and duties, including liability for damage?)
- Should we give robots the right to kill humans?
- Should we give robots the right to kill theirselves?
- Must Inventors Be Humans?
New EU rules for the ethical and legal status of robots and AI
On 16 February, 2017, the European Parliament’s Plenary Session adopted the JURI report on civil rules on robotics (European Parliament resolution of 16 February 2017 with recommendations to the Commission on Civil Law Rules on Robotics (2015/2103(INL))) with recommendations to the EU Commission on Civil Law Rules on Robotics. The resolution was based on an in depth report (DRAFT REPORT with recommendations to the Commission on Civil Law Rules on Robotics (2015/2103(INL)) that delineates core challenges of the evolving field of robotics, including the ethical and legal issues, and provides recommendations for the development of the field in Europe.
EU scientists denied robots legal personhood
As for 4 May, 2018, in an open letter, 156 artificial intelligence experts from 14 European countries have rejected the European Parliament’s recommendation that robots should have legal status as electronic persons. This would make robots responsible for repairing any damage they might cause.
- Floridi, L. & Taddeo, M. Romans would have denied robots legal personhood. Nature 557, 309–309 (2018).
- European Parliament resolution of 16 February 2017 with recommendations to the Commission on Civil Law Rules on Robotics.https://www.europarl.europa.eu/doceo/document/TA-8-2017-0051_EN.html
- IEEE Standards Association, Ethically Aligned Design, Version 2.https://standards.ieee.org/news/2017/ead_v2.html
There is a crane girder of a built-up I-section with span 12 m simply supported on two brackets. After 15 years of operation of double girder overhead crane, the girder is currently deflected downwards up to 3 cm at some points while the allowable deflection is L/800 which is 1.5 cm. What is the best action for this girder?
I am working on a paper in Conversation Analysis, titled “Toddlers asking Amazon Echo Alexa to play the song baby shark”
When analyzing the data i faced this issue of (when toddlers ask Alexa to play the song, and Alexa doesn’t respond to them, or doesn’t process their words correctly; due to their bad articulation,, what they do is repeat themselves again with the same exact words)
Is that considered repair or confirmation.
Videos‘ links are below for better understanding.
do someone has any resource about repairing leakage in as cast pipe and fittings?for example welding, powder or polymer repairing agents.
Most abdominal solid organ trauma (when the patient is stable) can be managed non-operatively (e.g. liver, kidney, spleen). However, it seems as though many complications of testicular trauma require surgical repair (e.g. testicular rupture, any penetrating injury, etc.). What might be the reason for this? Does it have anything to do with easy access (i.e. testicles are quite superficial)?
some of the Chinese manufacturers use special powder for repairing leakage of pipe and fittings after casting. they heating the pipe wall and then add this powder on the surface of leakage area.
How to fix the Error: The mesh file exporter could not resolve cyclic dependencies in overlapping contact regions. Please try to Repair Overlapping Contact Regions.
I'm working on inserting a 41bp sequence into a mouse medep cell using hdr, i'm wondering about the arm length of repair template.
I've read many guides, some say the arm can be 30-50bp, and the efficiency will decrease if the arm is longer. But some say it's better to use 50-80bp.
I wish i can get some advices. Thank you!
I would like to explore the possible topics to write a thesis regarding the Application of AR in Service Industry (in car repair workshops) any leads would be appreciated
Hi, I tried 2 conditions on two different samples of different concentrations, and one sample gives an ultra concentration, I have an Idea of whats going on here, but I want more experimented opinions.
1_ 1,08 ng/uL
2_ 2,57 ng/uL
I wanted to try if the ligation at Rt and at 20ºC makes some diference (20 minutes for all)
So I divided the samples taking 5uL each and diluting it to 5ng/uL with Elution Buffer, making 4 tubes, 2 tubes for sample so It ended up like this:
Then I went to end repair and A tailing, no problem at all.
On the adapter ligation I realized that on my tube of choice there was no volume for all samples (28nM final concentration) so I thaw a new adapter before using all the first one on 1.1, so I put the new adapter on 1.2, 2.1, 2.2.
1.1 and 2.1 went for adapter ligation for 20 minutes at Rt
1.2 and 2.2 went for adapter ligation on thermbloc at 20ºc for 20 minutes.
I´ve done the post adapter ligation cleanup with agencourt AMPure XP beads with no problem, I eluted the DNA with 20uL of elution buffer.
Then the samples went for library amplification for 12 cycles, and then I eluted the product again with the QIAquick MinElute kit columns & buffers.
Final concentrations goes like this (2uL samples on Qbit)
1.1- 3,32 ng/uL
1.2- 2,93 ng/uL
2.1- 6,96 ng/uL
2.2- 8.3 ng/uL
Is it possible that I didn´t resuspended well the new adapter after thawing it and after puting it in 1.2 so It was more concentrated and amplified more?
any other idea?
thank you so much!
I'm actually trying to transform two plasmids into yeast. 1. pRS416 with URA3 gene, 2.pRS413 with His3 gene. At first, i have transformed pRS416 into yeast that worked (got colonies on -URA plate). Then i have used these colonies, grew them using -URA media and tried to transform intact/linearized pRS413 using the same procedure. And plated them on -URA-His plates. Now my cells had (probably!) intact pRS416 and intact/linearized pRS413. After 3 days, I have observed colonies with intactpRS416+ intact pRS413 but i didn't see any colony on intact pRS416+linearized pRS 413.
PS: we know that plasmid pRS416 can produce a protein that will repair linearized pRS413 to make it intact again.
Amounts: plasmid=50ng, SS-DNA=100ug.
Anyone has any suggestion to transform linearized pRS413 into yeast cells? Thanks in advance!
I have conducted a field survey among 100 residential houses asking for the cost of repairs, the floor area, number of floors, age of structure, and I want to relate the cost to these parameters. What function would be best to fit the data?
our early experience with e-TEP for Inguinal Hernia is technically found to be easy even for Bilateral Hernia repair also.I am sure all Hernia surgeons will have different reasons .I feel that it is a promising approach.