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I am working with primary HUVEC cultures from Lonza. They are stored in liquid nitrogen at P5 in a freezing media of 90% FBS and 10% DMSO. They are plated on TC dishes coated with fibronectin in VascuLife EC media with VEGF at a density that has always been successful in the past. Media is changed the day after plating, and then every other day thereafter.
Sometimes the cells are able to proliferate and form a monolayer normally. However, in the past month, many of them experience death in large chunks of the plate starting the second day after plating (so after the first media change). For example, in a 6 well plate, one well may proliferate normally, two may experience almost complete cell death, and three will look normal in >50% of the well, but gone in the rest.
What is really confusing me is that they "balloon" out before death, a process that I am not familiar with. This occurs in cells handled by all lab members, so it is not one individual's technique in particular.
I have attached a 20x image showing a small area of healthy cells next to the sick ones.
Any guidance on how to remedy this issue would be greatly appreciated.

How can adsorption be integrated with other remediation techniques for more effective pollution control?
This question is arised for the determination of the weakness or gap of The Land Crimes Prevention and Remedies Act, 2023.
I am currently interested in these questions since the topic of 'money' seems to have been ignored in the broad field of intercultural communication education and research. What are your thoughts? Why might it be so and how to remedy this issue? Feel free to share your thoughts.
I am researching approaches to prevention and remediation in the field of cognitive science, particularly with regard to preventive interventions and applied remediation strategies. Any recommendations for relevant literature reviews or academic publications would be extremely helpful.
The study underscores a significant breakthrough in environmental biotechnology, particularly in the context of soil bioremediation using bacterial isolates from petroleum-contaminated soils. With the growing concern over environmental pollution due to crude oil spills, this research highlights the effective role of native bacterial species in degrading petroleum hydrocarbons, offering a sustainable approach to remediation. The study isolated and characterized two bacterial strains from the Baiji and Qayyarah refinery areas in Iraq, leveraging both phenotypic and molecular (16S rRNA gene sequencing) techniques to ensure accurate identification, with the sequences now registered under NCBI accession numbers LC596402 and LC596404.
These strains, belonging to the Bacillus genus, demonstrated a high degradation efficiency in a controlled setting using modified mineral salt medium with crude oil as the sole carbon source. Notably, at a 2% crude oil concentration, the isolates achieved impressive degradation rates of 78.19% and 86.5% for strains AM-I-1 and AM-I-3, respectively, confirmed via gas chromatography. This high degradation capability positions these bacterial strains as promising agents for bioremediation strategies, particularly in crude oil-contaminated sites.
For researchers focused on environmental restoration and sustainable biotechnological applications, these findings open avenues for further investigation into the optimization of bioremediation processes. This study not only highlights the biotechnological potential of these isolates but also sets a foundation for developing scalable and eco-friendly remediation solutions, which could be crucial for mitigating soil contamination in regions affected by crude oil pollution.
"What environmental and procedural factors could affect the efficiency of bacteria in degrading petroleum hydrocarbons, and how can experimental conditions be optimized to achieve higher degradation rates in natural contaminated environments?"
What are the different proportions needed for different ingredients in an one hectare pond for effective results?
I am trying to detach CFBE cells from tissue culture. I either use RIPA or cold PBS to scrab cells but I couldn't get the enough pelete
1. Should I use coating media because cells are not attached properly and removed by washing
2. Can I collect the media and don't discard it
Please give me any remedy
Can we stop global climate change? Does human scientific power reach the world's climate change? What is the response of the researchers?
As you know, humans are very intelligent and can predict the future climate of the world with hydrology, climatology and paleontology. But don't countries, especially industrialized countries, that produce the most harmful gases in the earth's atmosphere and think about the future of the earth's atmosphere? Do they listen to the research of climatologists? What would have to happen to get them to listen to climate scientists?
Miloud Chakit added a reply
Climate change is an important and complex global challenge, and scientific theories about it are based on extensive research and evidence. The future path of the world depends on various factors including human actions, political decisions and international cooperation.
Efforts to mitigate and adapt to climate change continue. While full recovery can be challenging, important steps can be taken to slow progression and lessen its effects. This requires global cooperation, sustainable practices and the development and implementation of clean energy technologies.
Human scientific abilities play an important role, but dealing with climate change also requires social, economic and political changes. The goal is to limit global warming and its associated impacts, and collective action at the local, national, and international levels is essential for a more sustainable future.
Reply to this discussion
Osama Behnas added a reply
Global climate change is impossible to stop. Human scientific power cannot reach the climate changes of the world.
Borys Kapochkin added a reply
Mathematical models of planetary warming as a function of the argument - anthropogenic influence - are wrong.
Alastair Bain McDonald added a reply
We can stop climate change, but we won't! We have scientific knowledge but no political will. One can blame Russia and China for refusing to cooperate, but half of the US population (Republicans) deny that climate change is a problem and prefer their promiscuous lifestyles to the answer:
All climate change is loaded on CO2 responsible for the greenhouse effect. Therefore, scientific experiments from several independent scientific institutions around the world should be conducted to determine what the greenhouse effect is at different concentrations of CO2. Then, a conference of a reputable and professional organization with the participation of all independent scientific bodies should be held to establish standards on CO2 concentrations and propose policy measures accordingly.
The second action that can be taken is to plant as many trees and plants as possible to breathe CO2 and release oxygen. Stop any deforestation and immediately plant trees in any tree-filled areas.
Lucy George added a reply:
We have the knowledge, tools and resources to ensure a livable and sustainable future for all. Carbon dioxide and other heat-trapping gases are major contributors to global warming. Therefore, reducing greenhouse gas emissions is very important and should be done as soon as possible to achieve zero greenhouse gas emissions. Both forests and oceans play an important role in regulating our climate, so increasing their natural ability to absorb carbon dioxide can also help prevent global warming.
Reply to this discussion
Ilan Kelman added a reply:
Yes, we can address and stop human-caused climate change. See extensive details in the full technical reports of ipcc.ch
Mohamed Sarmoum added a reply:
I think it is difficult to stop global climate change, but, on the other hand, we can develop adaptation mechanisms with this change
Mrutyunjay Padhiary added a reply
The challenge of combating global climate change is complicated and multidimensional, involving scientific, technological, political, economic, and social initiatives. Even though we may not be able to "stop" climate change entirely at this time, we can surely lessen its worst consequences and adjust to the changes that are already occurring. It is true that advances in science have allowed us to gain an in-depth knowledge of the mechanisms causing climate change as well as the tools and techniques that can be used to slow it down. Scholars from diverse fields such as ecology, engineering, economics, climatology, and social sciences are actively investigating climate change and devising remedies for it.
Sudhir Shukla added a reply
Global climate changes are at Macro- Mega scale changes basically induced by the continuing geological processes, hitherto invisible to present human generation because of their slow pace. The modern human race might have accelerated this change by adopting industrial expansion and ever-growing greed for conventional energy. Human effect is most visible in weather changes and weather anomalies more profoundly visible now-a -days when compared to global climate changes.
Think of climate changes in the past / geological history when human did not exist at all?
Be curious and want to learn something from cases
Book Title "Global Perspectives of Toxic Metals in Bio Environs: Bio-transformation, Health Concerns, and Recuperation“
We are pleased to invite you to contribute a chapter to our above mentioned edited volume to be published by Springer Nature. This book aims to provide a comprehensive and interdisciplinary overview of the current research on toxic metals in various biological environments, focusing on their bio-transformation, the associated health concerns, and strategies for recuperation and remediation. Interested authors may communicate their consent upto15-06-2024. Deadline to submit the chapter is 15-08-2024. Tentative Date of Publication shall be 25-01-2025.
Book Chapter Titles
1.Heavy metals in the Environment: The Global Scope
2.From Brick-and-Mortar to Biomarkers: The Evolutionary trend in heavy metal Detection
3.Unveiling the Pandora's Box: A Global Perspective of Toxic Metals in Bio environs
4.X-Ray Vision for Environmental Health: Advanced Spectroscopy Techniques in Heavy Metal Assessment
5.Geographical Variations: Mapping the Distribution of Toxic Metals Across the Globe
6.Perspectives of Biotransformation of Toxic Metals
7.Nature's Defence Mechanisms: Unveiling Biotransformation Pathways for Toxic Metals
8.Microbial Mediators: The Role of Microorganisms in Heavy Metal Biotransformation
9.From Friend to Foe: Understanding the Dual Nature of Biotransformation in Metal Detoxification
10.Nanotech to the Rescue: Engineering Innovative Materials for Heavy Metal Remediation.
11.Health concerns and issues associated with Toxic heavy Metals
12.Unveiling the Ecological Costs: Ecotoxicological aspects of Heavy Metal Pollution
13.A Silent Threat: The potential consequences of Toxic heavy Metals on Human Health
14.Bioaccumulation and Biomagnification: The Cascading Effects of Toxic Metals in the biosphere
15.Case Studies: Unveiling the Human Cost of Toxic Metal Exposure in Different Bio environs
16.Mapping Our Metallic Mess: Advanced Modelling for Heavy Metal Dispersion and Prediction
17.Recuperation Strategies and approaches for Contaminated Bio environs
18.Remediation Techniques: A Multi-pronged Approach to Reclaiming Contaminated Environments
19.Bioremediation: Harnessing Nature's Cleanup Crew for Metal Decontamination
20.Sustainable Solutions: Aligning Recuperation Strategies with Environmental Protection
21.Global Collaboration: Towards a Unified Approach for Mitigating Toxic Metal Threats
22.Emerging Technologies: A Glimpse into the Future of Toxic heavy Metal Management
23.A Call to Action: Safeguarding our Bio environs for a Healthier tomorrow
24.Towards a Heavy Metal-Free Future: Emerging Research Frontiers and Sustainable Solutions
Contact us:
Editors
1. Dr. Mohammad Aneesul Mehmood, Assistant Professor, Government Degree College, Shopian, J&K, India,
Email: aneesulmehmood@gmail.com
Mobile +91-9906681697
2. Dr. Rouf Ahmad Bhat, Research Scholar, Sher-e-Kashmir University of Agriculture Sciences and Technology Kashmir (J&K)
Email: rufi.bhat@gmail.com
Mobile: +91-7006655833
3. Dr. Gowhar Hamid Dar, Assistant Professor, Department of Environmental Science, Govt. Degree College, Kulgam, J&K, India
Email: dargowharhamid@gmail.com
Mobile +91-7006082223

The question seeks to identify whether the concept of diplomacy works as a mechanism that can remedy the current problem involve the invasion of a sovereign state like in the case of Russia invading Ukraine
I casted a polymeric film on hot plate but unable to detach it without damage,can any one suggest a remedy for this
what are the ways and solution in which there will be remediation to the environment, hazardous chemicals, and sediments in our society?
observation and suggestions are most welcome.
Hello intellect.
I am working with an algal consortium to treat wastewater derived from cattle (CRWW). Different dilutions of raw CFWW (FCWW/dH2O; v/v) were used for algal cultivation. As a control, the raw CFWW was taken at the same dilution without any algal inoculation. After batch cultivation for 10–12 days, I looked at the nutrient remediation profile and found that the control group—which did not have any algal cells—performed better than the phyco-remediation group because of its own microbial consortium.
Before treatment, the raw CFWW is extremely turbid, brownish in colour, and smells strongly. However, after batch completion, the colour of the phytoremediation case lightened relative to the control case, and both cases exhibited no smell. There is a range of 1200 to 1600 mg/L for the maximum soluble COD.
I need advice.
1. When dealing with raw, unsterile wastewater, is it a good idea to compare phyco-mediation with control (having microbes)?
2. Can I add a sterilization step before beginning phyco-remediation (such as using chemicals as our real-time treatment goal)?
3. Can we add any steps to the flocculation or coagulation process before the algal batch culture?
4. Alternatively, to improve microalgal doubling time and nutrient remediation as well, we must add some nutrients in the form of N/P.
5. Is there a competition between the algal cell and the natural microbiota of CFWW in the flask inoculated with algae, and if so, why does nutrient remediation become sluggish?
*****The microalgae consortium that was used as an inoculum was a CFWW-adopted culture that was in its active phase when it was added to the flask that had raw CFWW in it.
What specific herbal remedies have shown promising results in preventing or treating urinary stones, and what is the recommended approach for incorporating phytotherapy into a comprehensive treatment plan according to urinary stone type ?
Psychology too guarantees holistic healing and success in healthy life style.Astrology too mentions the same with remedies too .
I'm looking for a dataset that explores Ayurvedic health, including diseases, remedies, and formulations. The data should include information on traditional practices, herbal ingredients, preparation methods, and historical context. Any recommendations on where to find such a dataset would be appreciated!
Being a biotechnologist, I am always curious to know the real example where a stakeholder shows enthusiasm and implements an algal consortium or axenic culture to treat raw dairy wastewater (RDWW). If you are planning to implement this system, then you may face many barriers, like:
1. temporal change in RDWW
2. unsterile RDWW has its microbiota; maybe it competes with the algal consortium
3. Which cultivation system does it adopt: an open raceway pond or a closed system?
4. the flow rate of incoming RDWW.
6. Which mode of cultivation is more suitable for real-time RDWW treatment (batch or semi-continuous)?
7. After or before, which kind of operation enhances the remediation capability and is ready to use or discharge in natural water bodies?
8. How do you maintain the C/N/P ratio?
9. What about the smell of RDWW due to protein purification?
Hello everyone, We are doing a research project on using sewage treatment plant sludge in soil remediation and cooperation with researchers in this field is welcome.
Remediation of crude oil contaminated water by adsorption
Sometimes we are too much optimistic. Try to ignore small problems, thinking that they have no potentials to do any harm. such thinking gives us self-satisfaction and we continue doing nothing. Feel no pressure inside to understand the issue and their remedy. Ultimately no new creation.
Looking for your valuable comments and suggestions :)
I am currently working on wastewater remediation, using Fe based magnetic nanoparticles (MNPs). After reading literature, i understood that mostlly used nanoparticles are magnetite, maghemite & hematite. Many research papers mentioned use of coprecipitation method. After doing synthesis (as a beginner in research) what is the first step (or series of steps) i should follow after synthesis ? Pls guide
I purified my protein of interest using Ni-NTA chromatography. The protein was eluted but it eluted with a yellowish brown color which I have experienced before. I believe it was due to excess DTT (buffer mismatch) which led to leaching of Ni with my eluted protein.
Any ideas as to how to remedy this and remove the color (and Ni) without compromising the integrity of the protein.
Thank you.
One could argue that when R = KLaf ( Cs - C), the fugacity (the maximum transferable rate) has been reached and so the OTR or Rd is zero at that gas flow rate representing the energy input. However, this is a state that could not exist at that given gas flow rate, because OTR can never be zero in a respiring system. The case in which R = KLaf (Cs - C) is not a stable situation (i.e., not a steady state and C ≠ CR), which implies that the actual transfer rate, as opposed to total potential oxygen transfer rate, to the liquid is less than R and the DO concentration is decreasing because the consumption rate has exceeded the transfer rate so that the system is now outgassing oxygen. However, this does not mean Rd is negative. That is, the actual transfer rate, the net oxygen transfer rate given by Eq. (10a), i.e., OTR = KLaf (Cs – C) – r, is not KLaf (Cs - C). To maintain a given DO set point, the air flow rate (AFR) would have to be increased to a new KLa value such that ultimately R equals the actual transfer rate to the liquid. A change in air flow rate would result in a different transfer efficiency, at least in a fine bubble system where an increase in air flow decreases efficiency, and so the true C* also would be nominally different (higher, actually, due to lower gas side depletion), but eventually a steady state would be reached in which the oxygen consumption (R) would exactly match the oxygen transfer rate. For any further increase in the organic loading rate (OLR) and if C > 0, the system can respond by lowering C so that the driving force increases, giving more impetus to transfer. However, when the consumption rate exceeds the oxygen transfer rate, CR approaches C, which itself is ever-decreasing, such that dC/dt is a decreasing function of the consumption rate, i.e., dC/dt < 0. Therefore, the system is no longer in a steady state in such an event. Eventually a point is reached at which C becomes vanishingly small such that even the maximum fugacity is not enough to satisfy, and so the only remedy is to increase the gas flow rate again to match the demand. The conclusion of this exercise is that, for submerged aeration in which the gas loss rate from the system is significant, the rate of transfer under the action of microbial respiration must be given by Eq. (12b) i.e., dC/dt = Kla (Cs – C) – 2Ru, in which both the associated liquid phase oxygen equilibrium concentration (Cs) and the apparent oxygen saturation CR will decrease accordingly (such a phenomenon can be experimentally verified in a converse manner by a reduction in the microbial GDP (the resistance due to biochemical reactions), the net result of a dilution is that both the associated liquid phase oxygen equilibrium concentration Cs and the apparent oxygen saturation CR will increase accordingly. (It is notable that the latter increases faster than the former, so that at R = 0, the rise of CR catches up with the rise of Cs, and so both become one and the same, C*inff).
Mines' paper began on the right track by citing Bartholomew, Albertson and DiGregorio, and some others like Eckenfelder, that there is definitely a relationship between KLa and OUR and so Mines conducted his experiment. Herein lies the difference: Those previous researchers used plant operation data, where the DO is maintained constant. One can only have either constant DO or constant AFR (aeration gas flow rate), but not both. Mines' attempt to verify the dependency of Kla on OUR is premised on constant AFR which is exactly right but he used the wrong equation, resulting in Table 3 and Table 4 that yield the strange result that at steady-state, the OUR is not the same as the OTR. Had he used the right equation, he would have got a consistent result that would support my theory. The consequence of an increase of Ru can only be a reduction of OTR for a constant AFR. It can never by an enhancement! Mines' equation 6, stating that Rd = KLa (Cs- C) - Ru + Ri is therefore insupportable.
His experiment needs to be repeated, but with the following caveat:
equations must be correct, i.e., equation 7 must be written OTR = alpha KLa(beta Cs - C) – Ru resulting in the accumulation term as:
dC/dt = Kla (Cs – C) – 2Ru
OUR of the mixed liquor suspended solids as determined by Method 213B in Standard Methods must be modified to eliminate the shaking effect;
the OTR should be independently measured by the offgas method to compare with the modified Equation 7, since the offgas method is widely considered the best way to determine OTR.
It is important to recognize that the transfer equation given by Equation 1 in Mines' paper, is only valid when R = 0. When R changes, both Cs and OTR will change, even though C changes, (decrease to increase the driving force, or increase if the AFR increases).
Dans cet article , je suis co-auteur. Lorsque je tente de le poster sur Researchgate, mon nom s'affiche comme Pierre Mbang et un message renseigne que je n'ai pas de droit sur cet article. Comment pouvez-vous aider à resoudre cette difficulté?
I want to make a research on Effects of Partnership on Reading Remediation to raise level of Non Readers
Democracy places a lot of importance on numbers. Rabble rousers have also gained by it. Antony's speech in Julius Caesar is a good example. Poor Cinna the poet becomes a victim of the mob fury.If this event is taken to be the way democracy works, what remedy is available to the Cinnas of this world?
Fayalite and hercynite formed during the reduction of iron ore. What will be their effects ??
On the one hand, the author Ibram X. Kendi writes that "The only remedy to past discrimination is present discrimination. The only remedy to present discrimination is future discrimination."
On the other hand, the Supreme Court judge John Roberts holds that "The way to stop discrimination on the basis of race is to stop discriminating on the basis of race."
Which view do you side with -- and why?
What is the specific role of water-soluble fractions (dissolved organic carbon) of biochar in Hg Remediation? Please give your expert opinion.
Is it true that covid vaccines like Covishield, Covaxine etc has some side effects? if yes what are the remedies/what to do?
Learnard Scientist in this line may kindly share your suggestions for the greater benefit of human race. Thank you all.
Hey guys, can someone help me understand as to why the results are like this?
use a water and lead mixture to use as a synthetic Waste water and used water hyacinth to remediate the contaminant. My concern is that lead concentrations after the intervals are fluctuating most especially 1.36 ppm on the third testing where it increased by a little. Can someone explain why this happens or refer me some studies?

It is an aqueous mixture containing at least 6 components; of which more than 3 components are unknown !
Most of the papers submitted to the conferences have very low citations because they are not referred. This a great loss of a treasure of knowledge. The reasons for the same and remedies need discussion among researchers and hence I have selected the forum of ResearchGate .
The major difference between bimetallic and monometallic nanoparticles which can be applied for remediation purpose. In what context, the bimetallics are more useful than NZVI or monometallics even though NZVI has better removal efficiency. As per previously published literatures, NZVI can remove organic and inorganic contaminants up to ~90% and above. So, why will we use bimetallic instead of NZVI? By using which property of bimetallic, this can be used instead of NZVI? What could be the proper reason for using bimetallic instead of metallic for environmental remediation application? Please explain in details. Thank you.
Cosolvents, reactive membrane, chelants, complexing agents, or surfactants usage?
I am planning to do a research about mobile game-based strategy in teaching remedial classes in Math online. Since remediation only has about around 20 students, I can't do the two group pretest posttest control group research design. Thus, I am planning to compare how much they improved from Strategy 1(conventional) in the second quarter and Strategy 2(mobile game-based) on the next quarter, but the same participants will be observed. Is there a research design that fits to this idea? or is this format not possible?
I read on one-group pretest-posttest ( and one-shot) design but the many issues on such methodology make it unpublishable for education research papers.
There are a lot of soil remediation methods: surface capping, encapsulation, landfilling, soil flushing, soil washing, electrokinetic extraction, stabilization, solidification, vitrification, phytoremediation, and bioremediation. Still, I am looking for the most recent and the less investigated remediation method. It is better if the method could be easily conducted within a laboratory or greenhouse.
Thank you!
Traditional construction of research knowledge in master and doctoral thesis programmes / modules mostly comes from close interactions between research students and their supervisors. Most master and doctoral theses have sharp research foci. Post-doctoral research publications based on the research works might take longer time to be completed after graduation, if students still rely fully on their supervisors’ further guidance.
Luckily, some supportive or generous master or doctoral supervisors will help co-publish some journal papers with their research students, based on the thesis contents. Yet post-doctoral research works might not easily be offered under the same supervisors or the same universities. Without high-quality research publications, current young scholars ('baby-doctors') suffer badly from getting some post-doctoral scholarship or funded post-doctoral research projects, facing local and global keen competitions. Currently, mere doctoral degree cannot help much in most employment situations after graduation. Therefore, vacant post-doctoral teaching / researching job posts cannot be easily offered by universities or other higher education bodies in their own countries to young scholars after getting their doctoral degrees or even completing their postdoctoral studies in the slowly recovered local or international economic systems, especially under the violent attack of the COVID-19 pandemic.
To what extent can we (as university professors and lecturers) help remedy such situation, especially facing shortage of research fund? Any suggestions offered to our young scholars in local, international and global contexts?
For a doctoral candidate's lab work
For the remediation effect of electrokinetic technology, should we focus on ecotoxicity reduction or removal efficiency?
The 'blue cow', commonly known as "Neel Gai" (Boselaphus tragocamelus), once upon a time restricted to the hilly areas surviving mainly on the tree leaves and jungle grasses, has moved to the plain and has become major vermin in India. Some times they come in herd and destroy the entire crop leaving only the stubble. Though they prefer legume crops, during peak summer they eat every thing in such a way, as if there was no crop or vegetable grown there. This has become a major hurdle To farmers progress and their health in most parts of India, as the farmers are not able to grow the crop of his choice both for income and health eating which resulted in causing major loss to their income. The menace of these has cropped up recently and require immediate solution, The govt agencies must take appropriate action on the issue to safeguard the interest of the farmers.
As a scientist and bureaucrats let us find out the solution to the problem so that a consolidated draft could be forwarded to the authorities to check further loss. In my child hood I have seen people taming them and using these blue cow for ploughing. In Gir National forest of Gujarat they are best prey for lion. Please suggest the remedies to check the menace of these blue cows and also highlight their benefit to the farmers. Why we should not shoot them if they destroy our farm? govt should permit for the same,
Hello, Assalamu Alaikum!
I am a student of business administration. I am doing research on sound pollution. Because of sound pollution what kind of impact on people’s social life and business life and what kind of provlem people are facing and what is the remedy for it.
In this topic I wish your ideas cooperation and love. Thank you
I have seen that most researcher papers choose to use/focus only the Langmuir, Freundlich and D-R isotherm models (for the isotherm/adsorption study)
Why is it so ??
How about other isotherm model. Aren't they substantially/empirically important??
(Treatment of water )
Hello, I am a Nanotechnology student. I am currently working on my graduate thesis and I am interested in the synthesis method that you used for nanoceria. The only thing I would like to know, if possible, is what capacity was the autoclave used for the hydrothermal synthesis? Thank you for your time and I hope you can help me with that information. - Ciceli Escobar López
Can anyone list out the advantage and disadvantages of water pollution remediation technique/ method in a broad and detailed approach??
For example
UV treatment / Electrochemical/ biological process/ bioremediation
Membrane separation/ Advanced oxidation process/ coagulation or flocculation...
Adsorption by biochar/activated carbon/CNT/ Graphene etc.
Note: Need it for a write up. Thank You
Remediation of hydrocarbon impacted sites
I work with a protein that is normally well behaved, but I've recently developed the following intermittent issue: following cell lysis by sonication -- and multiple rounds of centrifugation at 16,000 x g and passing the supernatant through a 0.22-μm syringe filter -- the lysate still becomes cloudy after ~15 minutes and aggregates. This has led to debris ruining one of our affinity columns.
Why does this happen (intermittently) and what are the remedies?
Thank you.
All the problems arise on the fundamental balance of Nature. Once this balance is upset, it is very difficult to remedy it in a reasonable period of time, even if nature often takes back its rights when the effects are repairable.
As per as I know that a lot of researches are conducted on biocoagulant/ fungus for wastewater treatment or toxic metal remediation but I did not find find yet any product of commercial utilization . What are the difficulties of biocoagulant application in industrial level?
I have 2 models (negbin log and poi log) created using proc genmod in SAS. Both have overdispersion. Do I correct for overdispersion and then compare models again or do I compare right away choosing model with smallest deviance ignoring the overdispersion?
What is best remedy to be used with overdispersion? What does the remedy do to correct the situation? In re to former question, different remedies may affect things?
What is the possible solution or remedial action that should be taken for the Dionex Ultimate 3000 UHPLC - TCC 3000RS autosampler when there is a message "Syringe Driver: Position Error"? When self test is performed this message is coming and the autosampler module is not ready, even I tried to power off and on, but the error is occurred again.
Confusion about the concentration of metal ions and the mixing of metal with media and culturing procedure of bacterial isolates.
Assuming the multi-input single output data, the process simulation is to be implemented using the Adaptive-Neuro Fuzzy Model in MATLAB script. The data is to be presented in the numerical values stored as arrays.
The difficulty is experienced in the model, when the error says there is inconsistency in the output and input. The arguments in the input and output cannot match as there are five variables in the input and only variable in the output.
Please sort any remedy for this.
I am currently proposing a study on the metal uptake of a particular plant. In the study, I am going to spike my pot experiments in treatments with varying concentration of the metal of interest. One of the parameters I want to find is BCF. I am aware that it is a basically a ratio between the concentration of metal in roots/concentration of metal in the soil. I am confused however with this concentration of soil. Am I supposed to use the concentration of soil after the remediation or the concentration of the soil before the process in solving for the BCF? Thank you and God Bless!
Are there any natural remedies to get relief from Ankylosing Spondylitis?
If you are working in the Great Lakes especially in Areas of Concern (AOCs) we have a project that we are working on. This research project is to study the economic impacts of remediation and restoration at Great Lakes Areas of Concern. The research participant will conduct research collaboratively with ORD staff and the Great Lakes National Program Office (GLNPO) of EPA's Region 5 office. This research will apply a variety of economic approaches (e.g., both market and non-market valuation such as from I-O modeling, hedonic models, and benefit transfers) to quantify the benefits and costs of remediation and restoration projects in Great Lakes Areas of Concern (AOCs). The research participant will collaborate with a multi-disciplinary group of individuals including but not limited to water quality modelers, engineers, economists, ecologists and social scientists evaluating remediation, restoration and revitalization (R2R2R) outcomes that are central to attaining Sustainable and Healthy Communities. The R2R2R framework was developed by ORD & GLNPO to help fulfill Great Lakes Water Quality Agreement commitments “to restore and maintain the chemical, physical, and biological integrity of the Waters of the Great Lakes.” Potential endpoints of analysis include improved water quality, restored designated uses, and/or ecosystem services.
If you or anyone you know that is working on a similar project please contact me at:
When Valco has to switch to from A to B or B to A as per the method, error appears and batch sequence stops. Switching of the mass unit and restart is not helping much. Can someone suggest remedy.
I am attempting to run a Levene's test in SPSS. I have one DV and three IVs. I get the following error message:
c. Levene's Test of Equality of Error Variances is not computed because there are less than two nonempty groups.
What does this mean and how can it be remedied?
I'm wondering which type of casting defect has happened and what are remedies?

Dear Colleagues,
Which biases / measuring errors do I have to address when doing a survey, where the independent & dependent variable is collected via the same questionnaire from one person?
I already addressed common method bias by integrating a marker variable.
In particular any other biases / measuring errors I can address when setting up the questionnaire (like the remedy for common method bias with the marker variable)
Many thanks in advance!!
Xylene is used as a solvent in the printing, rubber, and leather industries. Which is the best and economical way of Xylene disposal? Is there any industrial waste management protocol for Xylene disposal?
My field of research is on remediation of crude oil polluted soil using organic amendment.My department is biochemistry.
Phytoremediation may refer as remedy of something with the help of plants, on the other hand bioremediation may define as the remedy of anything by using organisms or living things. In greater sense, Phytoremediation seems to be under bioremediation. Is it fact?
I believe the cost effectiveness of any remediation method as to do with it percentage efficiency and remediation capacity not the cost of synthesizing the material. I am currently working on remediation of PAH and selected heavy metals in contaminated soil. I need help on how to go about the above question.
biochar has been widely employed because of its appreciable potential as a soil conditioner to improve properties such as nutrient availability, microbial activity, water retention, crop yields, and carbon sequestration. However, recently, some reseach showed that biochar capping can be useding in control lake metal or nitrogen release. Whether biochar can be used in lake or river ecological remediation?
I am looking for recent data on the passive exposure of occupants of buildings containing asbestos. So far, I have not found much information on any measurement campaigns that have been conducted to find out about the background noise indoors (outside buildings where work or remediation is taking place).
How should economic growth help remedy or slow the inequality divide between low-income/no-come and high-in-come earners in any society?
Hello! I'm studying environmental engineering and would like to find the latest strategies and developments for DNAPL remediation and investigation, but don't know where to start.
Are there any websites or publications I can start with?
Many thanks in advance!
I am engaged in remediation of industrial wastewater using biological methods like phytoremediation, but I am confused in choosing a suitable plant for phytoremediation of industrial wastewater. Please can anyone guide me for choosing the suitable plant for phytoremediation of industrial wastewater? In addition, what criteria should be used in selection of plant too?
Are you aware of research-proven natural remedies that can be used to prevent mosquitoes bites (repellent), either by local application or consuming more amount of such food?
What about after the insect bite: any herbal remedies that is research-proven to be effective in reducing the symptoms?
Non steroidal anti inflammatory drugs has been found to have a major role in decreasing edema and inflammation of vessels wall and permit air entry to lung, what is the clinical parameter that indicate it is uses?
Dear Researchers,
Whether the new mutant Covid-2.0 going to effect another big crisis in the new year 2021?
What would be the possible remedy?
Thank you in advance for sharing your thoughts.
Wish you all a very happy new year.
i just want to know that kitchen waste is how much efficient in heavy metals remediation when we add kitchen waste with heavy metals contaminated soil.
Dear Fellow members,
This is related to the soil contamination with petroleum and diesel having high level of TPH up to 5 - 7%. I would like to know some references on the following subjects:
1. Bench Testing of soil remediation to assess the level of contamination before and after Remediation
2. The best microbial product used for soil bioremediation
Appreciate the prompt response.
Hello researchers
I am conducting research on heavy metal contaminated groundwater remediation using nZVI, a batch lab experiment using the stock solution will be done but I want to design a laboratory-scale pilot to simulate the real conditions of in situ remediation how can I do it???
Notice: my aim is to study process parameters not the nzvi transportation
The number of people infected with the new coronavirus continues to skyrocket, with more than 80000 cases worldwide as of the end of February. But there's no vaccine or cure in sight, meaning that doctors can do little more than offer supportive treatment to the very sick and hope their bodies can survive the infection.
As the scientific community scrambles to find a drug that can effectively treat patients sickened by the new coronavirus from China, doctors are trying some surprising remedies: medicines targeting known killers such as HIV, Ebola, and malaria.
How?
When?
Despite of having several law in force across countries, corporate frauds continue to occur. Even IT and ITES have come in a bigger way to support the industries, still frauds occur. Where is the remedy then?
Can anybody let me know about the black dots present between the HepG2 and Hepg2.2.15 cell line? Remedies to remove it. After PBS wash, it is removed.
How can we minimise the rate of corruption in developing countries or while availing any other public services?
What according to you can be the best remedies through which it's growing rate can be reduced or minimised?
Sir
I am in a process of analyzing the data for developing a multi-regression equation. The only problem I encounter with is the Durbin-Watson value which comes well below 1. Please suggest the remedy.
Thanks and regards
Kaushar Ali
Hi everybody: i am looking for recent datas about the amount of registered biocides containing silver nanoparticles. The most recent data i found is in this paper from Nowack et al. (2011) which explains that about the 53% of biocides registered at EPA contains nanoAg and the 7% of it contains AgNPs. I looked for more recent datas/numbers about biocides cointaining nanoAg/AgNPs, but from main sites like EPA's, REACH's and ECHA's or from the huge amount of papers i read nothing came out. I was wondering if someone has something (papers, reviews, sites etc..) to share with me to help my research. Everything is accepted but it must be referred to biocides containing nanoAg/AgNPs.
Thanks a lot to everyone who will share his/her time with me
Mattia
i want to know how catalase,Glutathione and ascorbate peroxidase enzymes play role in metal accumulation and extraction
Sri Lankan Indigenous Medicine is an assert to Sri Lankan health sector. In addition to its healing effect, SLIM has social and cultural values which are worth studying, researching and surveying. SLIM has remedies for most of the current burning issues in health field such as cancer and other NCDs.
I have an ARDL error correction model, in the CUSUM chart as far as I diagnosed there is a negative trend, moving means. Before that, I found that the explanatory variable and the dependent variable are not integrated to the same degree. I know I could not use ECM when there is no cointegration, I don't know is there any other model that gives more consistent results.
Could anyone offer a remedy?

A farm owner has approached me with a grievance on the yield loss in Sapota (Chiku) fruit in Dahanu district, Maharastra, India (tropical climate). Trees often get infected presumably by a virus, leaving the fruits corky brown (please refer to the attached images). Farmers have tried using the required insecticides to prevent any insect source of the virus, however, there has been no improvement. Unfortunately, the related agriculture agencies could not identify the exact cause of this disease so as to suggest an appropriate solution. The disease is rampant and there is a drastic reduction in the total yield year after year.
If an expert is reading this, please suggest.


What are the possible remedies and solutions for Neck Blast disease in paddy?
Wants the related research journals on correlations hip between remedial education and performance of physics.
Sea water was polluted with crude oil and requires remediation, can coexixt 9500 and 9527 increase microbial population to aid the remediation of this pollutant?
I am trying to take sequential soil samples of my 3L greenhouse pots over the course of my plant's lives. I was using a 2 cm diameter soil corer for the pilot study, but it created too large of holes in my pots. I have thus-far been unable to find a tool for extracting soil cores from my pots with a smaller diameter than 2 cm. Does anyone have any suggestions on a tool I could use to remedy this issue? The soil samples are for DNA analysis and nutrient content analysis.
Thank you!
We researcher have predicted and wrote in our writings but authority did not take any remedial action for safe the coastal population by developing embankment/bank in coastal belt of Bangladesh. Finally it has happened that suspected to be happened!
See the links-
In other words, how can you predict, prevent, and remedy the land degradation occurrence?
Phytoremediation of polluted sites through trees.