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Reliability - Science topic

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Hello
I am particularly interested in obtaining historical data for an artificial intelligence index. If anyone has access to this type of data or can recommend sources where I might find it . I would be very grateful. Information on datasets, APIs, or reliable databases for AI indices would be incredibly helpful for my research.
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Free public data:
PUBLIC DATA: 2024 AI Index Report - Google Drive
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Considering that traditional situational judgment tests (SJT) often demonstrate not high reliability but can be valid, is it possible to develop methods or approaches that would enhance the reliability of SJT without compromising their validity? What changes in testing format or assessment methods would you recommend to achieve this goal?
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Hi Andrew,
I am no expert but I strongly doubt that SJTs have low reliability. Isn't it rather that they show lack of internal consistency (as ONE approach to reliablity)? I would simply try to repeate the test (retest rel) and would predict that the scores are pretty solid.
Best,
Holger
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Hi all,
I'm looking to conduct screening on a cell line of interest, using the Boyden Chamber Assay method, to identify migratory vs non-migratory phenotypes and then elucidate their matching genotypes.
I was wondering if anyone had any ideas of how to reliably separate the migrating cells on the lower transwell membrane, from the non-migratory cells suspended in the upper chamber?
Any ideas are appreciated...thanks!
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you can deploy Copilot Separating migratory and non-migratory cells in a Boyden Chamber Assay can be a bit tricky, but here are some steps and tips to help you out:
### Steps to Separate Migratory and Non-Migratory Cells
1. **Prepare the Boyden Chamber:**
- **Upper Chamber:** Place your cell suspension in the upper chamber.
- **Lower Chamber:** Fill the lower chamber with a medium containing chemoattractants to encourage cell migration.
2. **Incubation:**
- Allow the cells to incubate for the appropriate time to enable migration through the membrane.
3. **Collecting Migratory Cells:**
- **Remove the Upper Chamber:** Carefully remove the upper chamber without disturbing the lower chamber.
- **Wash the Membrane:** Gently wash the membrane to remove any non-migratory cells that might be loosely attached.
- **Scrape the Membrane:** Use a cell scraper to collect the migratory cells from the lower side of the membrane. Alternatively, you can use trypsin to detach the cells.
4. **Collecting Non-Migratory Cells:**
- **Upper Chamber Cells:** Collect the non-migratory cells that remain in the upper chamber by gently pipetting them out.
### Tips for Reliable Separation
- **Gentle Handling:** Be gentle when handling the cells to avoid disrupting the membrane or causing cell damage.
- **Optimal Pore Size:** Ensure that the pore size of the membrane is appropriate for your cell type. Typically, a pore size of 8 µm is suitable for most cell types.
- **Staining:** Stain the cells to differentiate between migratory and non-migratory cells. This can help in visualizing and counting the cells accurately.
- **Validation:** Use complementary techniques like flow cytometry or microscopy to validate the separation and ensure the purity of the collected cell populations.
### References
- **[Cell Migration and the Boyden Chamber](https://link.springer.com/protocol/10.1385/1-59259-137-X:047)**
- **[Discussion of Cell Migration Assay Formats](https://www.cellbiolabs.com/news/discussion-cell-migration-assay-formats)**
These steps and tips should help you effectively separate migratory and non-migratory cells in your Boyden Chamber Assay.
Good luck
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Greetings to all.
My laboratory is currently seeking funding and financing and the opportunity has arisen to acquire a device for determining food composition (DA 7250 NIR Analyzer).
I would like to hear the opinion of food science peers to verify whether the results obtained from this technology are reliable and do not require the use of traditional techniques (e.g., protein determination using the Kjeldahl method).
Thank you!
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NIR technology is useful to determine the proximate composition of food/feed.
However, the method has lower performance than a chemical analysis method and is highly dependent on the algorithm and even more on the robustness of the dataset on which the calculation is based.
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I am trying to calculate the reliability of a product(like multiple devices of the item), I would like to know what are the various techniques that can be used and different methods of determining an ideal failure situation. I am already aware of the different indices like MTBF, MTTF, basic Reliability index for a single item of a product. I appreciate your suggestions and help. Thank you.
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Thank you for all the insightful answers. I will keep your suggestions in mind proceed with the analysis. Jean-Pierre Signoret Anthony Bagherian Steven Cooke
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I have a question about reliability/agreement.
100 raters (teachers) evaluated 1 student presentation based on 5 criteria (introduction, eye contact, etc.). Each rater rated each criterion. Each criterion was rated on a 5-point interval scale.
I would now like to calculate the inter-rater reliability for each criterion individually. In other words, to what extent do the teachers agree on the assessment of the criterion of eye contact, for example.
Since I only want to analyze the reliability of 1 item, I believe that many common reliability methods are not applicable (Krippendorf's alpha, ICC).
So my question would be how I could calculate this agreement/reliability? Thank you very much for your help.
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Inter-rater reliability tests do not apply to evaluating single items by multiple coders. Consider using intra-rater reliability tests instead.
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https://konnifel.com/. Specifically, how reputable is it for offering valuable research experiences, and what feedback have students and professionals given about its internships?
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ANY internship experience and value always depends on the student as well as the professor. "Brand" names are less impressive than they used to be. Demonstrating what you have learned and how you do research is most important to future collaborations or positions. CRITICAL to success is a good alignment of any researcher's interests and the intern's! Promotion of student contributions and co-authorship are essential. This applies to ANY internship at any institution. One person's experience may not reflect another's as they are all unique. The BEST way to evaluate it is to see if you can find "graduates" - people who have interned there - and try to contact them directly to get their candid evaluation of the programs and results.
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Prior to drafting, I was given the chance to use either a quantitative or mixed methods approach. Due to me consulting with teachers and a former panelist before. However, as I am writing my undergraduate study it becomes apparent that I could not utilize a theory due to the limitations of my study (i.e., learning curve of learning a software or a research instrument and determining how reliable it is, financial capacity of the researcher and time frame for a simultaneous course such as mine). Also I would like to add that the 3 theories considered were rejected due to this: the first one had a variable that is beyond the scope of the study; second and third both had unclear definitions and would be a source of uncertainty; plus the third one had math topics that are too hard for me such would only exacerbate the concerns of learning curve and the time frame. Besides that (I am not sure of this) the research question that I had was exploratory in nature so even if I did use one or two theories I am hesitant if it is truly necessary.
So I am wondering if my understanding is correct, does an exploratory research not warrant a theory? If it does, is it acceptable of me not to utilize one given my limitations ?
On another note, given how quantitative study is always about theory testing should I go for a mixed methods approach and state the assumptions of my study as follows:
Qualitative assumptions:
1. The research generates meaning as he or she interacts with the study and its context.
2. Researcher finds pattern when collecting data from or for a specific problem.
3. Theory generation. (Not to be included)
Quantitative assumptions:
1. Theory testing (Not to be included)
2. Knowledge is antifoundational.
3. Data collection, know how and rational considerations create knowledge.
Would it be acceptable for me to use an exploratory sequential mixed method? Is it okay for me not to use either theory generation or testing as I find it difficult to find the middle ground between the two and just present it as a research gap?
I am quite confused at the moment. Inputs would be highly appreciated. Thank you madams and sirs.
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I do not know enough about your substantive research question to be able to say whether a non-directional hypothesis is appropriate. In essence, a non-directional hypothesis says that result could go in either direction, for example a correlation could be either positive or negative.
If you do pursue an exploratory sequential design, one possibility would be to conclude by developing and possible pretesting the relevant measure(s) for a survey.
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A software that can be downloaded or available freely for students.
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I use PyRx, it's simple and can be easily taught to new students. It does not require any coding skills and the data can be visualized in Chimera or discovery studio. It's fairly cited and I have published data using them.
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As a graduate student at the Arizona State University in the Mary Lou Fulton College of Education, I am beginning my Capstone process. In one of our classes, we have been asked to develop a definition for "Research" and "Evaluation" in our own words.
Research and Evaluation
· Research is the collection of information with the intent of increasing knowledge and understanding on a specific subject.
· Evaluation is the practice of judging the performance to a specific set of criteria or standards for accomplishment.
To compare and contrast "Research" and "Evaluation" if noticed these specific items.
Compare and Contrast
· Similarities – Both Research and Evaluation should be grounded in empirical evidence. In each, reliable and valid evidence is collected for analysis.
· Differences – The purpose of research is to collect information to explain existing bodies of knowledge or generate new theories. The purpose of evaluation is to assess the understanding or performance against a specified standard.
In your experience as educators or professionals, is there marked differences between these concepts or have they become synonymous?
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Academic research and evaluation both involve systematic inquiry, but they differ in purpose, scope, and methods. Academic research aims to generate new knowledge, explore theories, or address unanswered questions, often contributing to broader scholarly discourse. It emphasizes theory-building, hypothesis testing, and rigorous methodologies, with findings typically shared through peer-reviewed publications. Evaluation, on the other hand, focuses on assessing the effectiveness, efficiency, or impact of a specific program, policy, or intervention to inform decision-making. While both use qualitative and quantitative methods, evaluation is more applied, context-specific, and results-driven, offering actionable insights for stakeholders. A key similarity lies in their reliance on data collection and analysis to draw conclusions, but academic research prioritizes knowledge creation, whereas evaluation emphasizes practical application.
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What is the difference between performance, stability, and reliability in the context of estate appraisal?
Is any performant (accurate) appraisal necessarily reliable? Stable?
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"Applicable" refers to the suitability for a particular use. "Adaptable" means that the same methods/algorithms can be used for somewhat different uses with little or no modifications. Something that is adaptable would be applicable for different situations. Something that is applicable for one situation may or may not be applicable when the conditions change. It would not be adaptable in that case.
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we need a research scholar who has done factor analysis before.- EFA,CFA,reliability and Validity cheeking of items, and stability in SPSS or SPSS Amose.
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i want to know more about you .can you please tell me more how you can be a perfect team member on this work?
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I cannot seem to find a source that sells these cells… And the papers I've read so far a not clear concerning cell line origin. It's always someone else that gave them a vial.
Assinante is highly appreciated.
Kind regards,
Vasco
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Dear Vasco Branco ,
i am also looking for this cell line. Did you find a reliable provider? I would be very thankful to hear from you!
Thank you and best regards!
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Salam Alaykoum,
I am opening this discussion to gather insights and perspectives regarding the use of Our World in Data (OWID) as a source for scholarly articles, research, and studies. OWID is a widely used platform that provides accessible data on a range of topics like global health, economics, environment, and education.
Regards,
Dr. F CHELLAI
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'Our World in Data' as a Resource for Articles and Studies" has its strengths, including its transparency in data sourcing, collaborative efforts with experts, user-friendly visualizations, and a focus on global context. These features enhance the platform's reliability and accessibility for researchers, policymakers, and the general public. However, it also has several limitations which include data limitations and gaps across various topics, the potential for misinterpretation of data by users, the dynamic nature of continuously updated datasets, and the limited scope of some indicators, particularly in social sciences. Users of this platform should be advised to be cautious of these limitations when utilizing OWID for academic or journalistic purposes. users must remain discerning and attentive to the evolving nature of the information they consume and analyze.
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I'm trying to implement BB84 on a network, however I don't have a source code that is backed by any organization or a peer reviewed paper. Any help would be appreciated.
Thanks!
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@Mario Stipčević, I'm doing my research project in the field. Hence, the need for source code. I've built my own, however I need to benchmark it against some other code/results and look for any improvements.
Could you suggest any sources or directions I can go in?
Thanks!
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Can somebody tell me about the good books of reliability engineering?
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Please provide link/s.
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Although it may seem like a straightforward task, several labs have struggled to find a reliable vendor for freezing stage microtomes. Has anyone recently sourced one from a supplier, excluding eBay or second-hand options?
The aim here is to use freezing stage microtome to cut brain sections.
Thanks a lot!
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Bright Instruments has quality equipment and user friendly. Also you can check the big companies (Thermo Scientific, VWR, etc)
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Within the framework of Research 5.0, the incorporation of AI-driven approaches holds the capacity to reshape the field of scientific investigation profoundly. This paradigm shift in research practices has the potential to improve the precision of data analysis, optimize the efficiency of research procedures, and maintain the utmost ethical standards in diverse fields of study. The ability of AI to analyze vast amounts of data, identify patterns, and create predictive models enables academics to gain insights with unparalleled accuracy and efficiency. Furthermore, the ethical integration of AI in research guarantees openness, impartiality, and responsibility, effectively dealing with issues related to prejudice and data reliability. With the growing importance of AI in Research 5.0, it holds the potential to fundamentally change the processes of knowledge generation, validation, and application in academic and practical settings.
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AI-driven methodologies in Research 5.0 have the potential to significantly transform the landscape of scientific inquiry by enhancing accuracy, efficiency, and ethics across various disciplines. Here’s how:
1. Accuracy
  • Data Analysis and Pattern Recognition: AI algorithms, particularly those based on machine learning, can analyze vast amounts of data more accurately and quickly than traditional methods. They can identify patterns and correlations that may be missed by human researchers, leading to more precise and reliable results.
  • Predictive Modeling: AI can create sophisticated models that predict outcomes and trends based on historical data, improving the accuracy of forecasts and simulations in fields like climate science, epidemiology, and economics.
  • Error Detection: AI tools can automatically detect inconsistencies and errors in data, reducing the likelihood of human error and ensuring the integrity of research findings.
2. Efficiency
  • Automated Data Collection: AI-driven tools can automate the process of data collection and processing, saving time and reducing manual effort. For example, AI can be used to scrape and organize data from scientific literature or experimental results.
  • Enhanced Research Workflows: AI can streamline various aspects of the research workflow, from literature review and hypothesis generation to experimental design and result interpretation. This accelerates the research process and reduces time to publication.
  • Personalized Recommendations: AI systems can recommend relevant research papers, methodologies, or data sets based on the researcher’s interests and needs, facilitating more efficient literature review and knowledge acquisition.
3. Ethics
  • Bias Detection and Mitigation: AI can help identify and address biases in research data and methodologies. By analyzing large datasets for potential biases, AI tools can assist researchers in making more objective and fair assessments.
  • Ethical Decision-Making: AI-driven systems can provide guidance on ethical considerations and best practices in research design, data handling, and reporting. This includes ensuring the responsible use of data and adherence to ethical standards.
  • Transparency and Reproducibility: AI can enhance transparency in research by providing detailed records of data processing and analysis steps. This promotes reproducibility and accountability, as others can verify and replicate findings more easily.
Applications Across Disciplines
  • Biomedical Research: AI-driven methodologies can accelerate drug discovery, optimize clinical trials, and personalize medical treatments by analyzing genetic data, predicting disease outbreaks, and identifying new therapeutic targets.
  • Environmental Science: AI can improve climate modeling, monitor environmental changes, and optimize resource management by analyzing satellite imagery, sensor data, and ecological models.
  • Social Sciences: In fields like sociology and economics, AI can analyze large-scale social data, predict social trends, and evaluate the impact of policies by processing complex datasets and identifying patterns in human behavior.
  • Material Science: AI can facilitate the discovery of new materials and optimize manufacturing processes by predicting material properties and simulating experiments.
Challenges and Considerations
  • Data Privacy: Ensuring the privacy and security of sensitive data used in AI-driven research is crucial. Researchers must adhere to data protection regulations and ethical guidelines.
  • Interpretability: While AI can provide accurate results, understanding and interpreting these results can be challenging. Researchers must ensure that AI methodologies are transparent and that their findings are interpretable.
  • Dependence on Quality Data: The effectiveness of AI-driven methodologies depends on the quality and diversity of the data used. Poor-quality or biased data can lead to inaccurate or misleading results.
In summary, AI-driven methodologies in Research 5.0 have the potential to revolutionize scientific inquiry by enhancing accuracy, efficiency, and ethics. By leveraging advanced AI techniques, researchers can achieve more precise results, streamline workflows, and address ethical considerations more effectively, leading to more robust and impactful scientific discoveries.
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I sequenced two isolates of a virus and constructed a phylogenetic tree base on their partial sequence. Although both sequences are 100% identical, they are separated from each other by another NCBI sequence that has 99% identity to my sequences.
However, the number of sequences submitted in GenBank is limited (about four sequences) and when I constructed the tree based on a shorter sequence (but more sequences), this problem will be solved.
Is it possible the low number of sequences cause this issue? and which tree is more reliable? a tree with more sequences but shorter length or a tree with low number of isolates but longer sequence?
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This question refers to something from a long time ago, so I don't recall exactly what I did. However, I suppose the sequences were incorrect. I replaced them with the correct sequences, so you may want to check the accuracy of your sequences.
Another issue might be related to selecting an inappropriate outgroup. Make sure to choose one that is significantly distant from the other sequences.
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Dear Researchers:
when we do the regression analysis by using SPSS, when we want to measure a specific variable, some researchers take the average of items under each measurement while some others add the value of each items ? Which one is more reliable? which one produces more better results ?
Thanks in advance
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Summing really is not a great approach unless the summed scores are scaled in a way that’s well known in application (and even then is problematic).
The mean has two advantages. 1) If the items have a fixed range interpretation is usually easier. If it’s a 1 to 7 scale then 1 is lowest, 7 highest and 4 in the middle. A summed scale is hard to interpret because of variation in number of items. 2) With missing items the sum treats missing as zero so it distorts the score. The mean treats them as if missing completely at random. This is not perfect but better than treating values as 0. Also with only a few missing items and problems are minor.
Ideally one would impute missing values but I’ve seen quite a few analyses that sum scores with missing data and end up with essentially garbage outcomes (if 0 is an impossible value it can really mess with results).
If there’s no missing data the analyses will be identical except for the interpretation issue. So generally the mean is the better and safer default.
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For the seismic design of structures, building codes typically consider an intensity corresponding to a ground motion with a return period of 475 years. This return period implies a 10% probability of exceedance over the structure's lifetime (usually 50 years). Given that failure is equally likely in each year (assuming independence), the annual probability of failure is calculated to be 0.0021 per year.
I am curious about how this specific number was determined and which was the first code to propose it. Is this value based on a quantitative assessment, or was it a consensus decision within the engineering community? More importantly, how does this annual failure probability relate to the target probability of failure in codes like EN1990, which explicitly indicates a target reliability index of β = 4.7 (corresponding to a failure probability of 10^-6 per year)?
If anyone can share their insights on this topic or point me toward relevant references, I would greatly appreciate it.
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Hi, suggest read this...
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Hello everyone,
I am researching AI-enabled systems' impact on user interaction and experience. Specifically, I want to understand how different AI technologies (such as computer vision, natural language processing, and machine learning) enhance user engagement and satisfaction in various applications.
Here are a few questions to kick off the discussion:
What are the key factors that influence user interaction with AI-enabled systems?
I’m looking to identify the various elements that affect how users interact with AI systems, such as user interface design, accuracy, reliability, ease of use, and personalization.
How do these systems improve user experience compared to traditional systems?
I am interested in comparing AI-enabled systems with traditional, non-AI systems regarding user experience. How do AI systems provide more intuitive and responsive interactions, offer personalized recommendations, and automate routine tasks?
Are there any notable case studies or research papers that highlight successful implementations of AI in enhancing user interaction?
I would appreciate references to existing research or case studies demonstrating successful AI implementations in improving user interaction. Examples from healthcare, education, or customer service would be precious.
Any insights, references, or personal experiences would be greatly appreciated!
Thank you!
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I work with a technology called PEGA which is a Business Process Automation tool. I have seen use of Natural Language Processing and AI in Customer Service applications to help the agents identify the next best actions while talking to the customer. This improves the quality of service provided by providing the agent with tools to assist the customer proactively and this reducing the interaction time. AI can also be used in knowledge management in Customer service applications specifically in Healthcare and Insurance industries.
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I am Ph.d scholar I have read from some university guideline that ... 2.2 Hypotheses (if applicable): Hypotheses must be formulated in light of the research questions and objectives of the study , which are to be tested for possible acceptance or rejection .
it is said in the following manner...
Cronbach's alpha is a reliability test that measures the internal consistency of a questionnaire or survey. It ensures that the questions are measuring the same concept or construct, and that the results are consistent and accurate.
Here's how Cronbach's alpha justifies the absence of a hypothesis:
1. *Exploratory research*: If you're conducting exploratory research, you might not have a pre-defined hypothesis. Cronbach's alpha helps ensure that your data is reliable and consistent, allowing you to explore patterns and relationships without a preconceived notion.
2. *Pilot study*: Cronbach's alpha is useful in pilot studies to refine your questionnaire or survey. By ensuring reliability, you can make adjustments before conducting the main study, reducing the need for a hypothesis.
3. *Descriptive research*: In descriptive research, you aim to describe a phenomenon without testing a hypothesis. Cronbach's alpha ensures that your data accurately describes the population or phenomenon.
Examples:
1. A survey to understand customer satisfaction with a new product. Cronbach's alpha ensures that the satisfaction questions are consistent and reliable, allowing for accurate description of customer satisfaction.
2. A questionnaire to explore the impact of social media on mental health. Cronbach's alpha ensures that the questions related to social media use and mental health are consistent and reliable, enabling exploration of patterns and relationships.
3. A pilot study to develop a new scale measuring employee engagement. Cronbach's alpha helps refine the scale, ensuring that it's reliable and consistent before conducting the main study.
In summary, Cronbach's alpha ensures the reliability and consistency of your data, allowing you to:
- Explore patterns and relationships without a pre-defined hypothesis
- Refine your questionnaire or survey in pilot studies
- Accurately describe phenomena in descriptive research
By justifying the reliability of your data, Cronbach's alpha supports the absence of a hypothesis in your research.
So, plz someone help me for my question after reading the above discussion.
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Descriptive research does not have any hypotheses.
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I have some doubts about the reliability and accuracy of the observations. Some insect observations and other taxa may require expertise or PCR analysis for accurate identification. Have you used iNaturalist for research before? Any recommendations or stories about it?
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Hi Ismael,
I use it, and know others who do, and the answer is the vague "it depends" - it depends on who is doing the identifications; and it depends on what you want to use the data for. For me, I've found users sometimes take superb photos of my group (mites) that I am working on; I've reached out to some users and worked together on identifications; I have "cleaned up" some taxa I know well; I have seen invasive species move through Australia thanks to users taking photos. There's plenty of like-minded nature lovers on it who really appreciate a scientific interest in their photos.
So it's useful, despite mites being one of the worst groups for identifications, with the AI suggested identification often being wrong, and most photos being understandably poor for proper identifications. If you work within those bounds, it's still a surprisingly useful resource (and fun!).
However, when you get a group of reliable experts identifying groups that can be done via photos, the data on the whole can become superb. Australian dragonflies are one such example. Vet the data yourself, then see if it can answer your questions. Also, if you've got expertise in a certain group, join up and lift the standards!
all the best, Owen
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What methods do you use to gather data in a case study, and how do you ensure its reliability?
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Hi
I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model.
Thanks
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Thank you very much Shafagat Mahmudova, for your guidance
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Hello, I am trying to fix Pseudomonas aeruginosa PAO1 cells with 4% formaldehyde for 20-30 minutes (on ice and in the dark) for fluorescent microscopy. I am attempting to stain the DNA and outer membrane, but have not been successful with this fixation method. The dyes give weak signals and appear to be exported out, suggesting the bacteria are not completely fixed with formaldehyde. Does anyone have a reliable protocol for PAO1 fixation, including concentration, time, quenching, etc.? Any help would be greatly appreciated.
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Unfortunately all of my work has been on mammalian lines.
Good luck with the fixation
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I need a reliable source or an example supported by excel sheet to understand Fuzzy Vikor?
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Fuzzy VIKOR is a decision-making method used to rank and select from among alternatives that involve conflicting criteria. It extends the traditional VIKOR method by incorporating fuzzy logic to handle uncertainty and vagueness in the decision-making process.
Here's a brief overview of the Fuzzy VIKOR method:
  1. Define the Decision Matrix: List the alternatives and criteria, and evaluate each alternative under each criterion using fuzzy numbers (usually triangular fuzzy numbers).
  2. Determine the Best and Worst Values: For each criterion, determine the fuzzy best (ideal) and worst (anti-ideal) values.
  3. Calculate the Separation Measures: For each alternative, calculate the distance from the fuzzy best and worst values, considering all criteria.
  4. Compute the VIKOR Index: Calculate the VIKOR index, which combines the maximum group utility and the minimum individual regret. This index is used to rank the alternatives.
  5. Rank the Alternatives: Based on the VIKOR index and a compromise ranking, alternatives are ranked, and a compromise solution is determined.
To illustrate this with an example, let's consider a simple scenario with three alternatives and three criteria. The alternatives could be different suppliers, and the criteria could include cost, quality, and delivery time. We would assess each supplier on each criterion using fuzzy numbers.
Would you like me to create a sample Excel sheet with data and calculations to demonstrate the Fuzzy VIKOR method? If so, please specify the alternatives and criteria you'd like to use, or I can generate a generic example for you.
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Who is controlling it? Are there any reviews?
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Thank you Steven. I am still puzzled by the origin of those discrete "professional meteorologists and climatologists" who created that site.
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I have identified many solutions. I need suggestion from somebody with application experience of this topic to identify the most reliable and robust procedure.
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I read you are in Chennai. I was many times there in the past to collaborate with NIOT. Many thanks again for your clear and complete suggestions.
Daniele
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Hi! Me and my mates are conducting a correlational study. We wanted to gather 150 participants as a minimum number and a max of 300. The problem is that I am having a hard time finding reliable citations that could explain 150 respondents are Okay.
hope to have an answer soon. Thank you!
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In a correlational study, the sample size can significantly impact the reliability and validity of the results. While 150 respondents can be a reasonable number, it's important to consider the context, the expected effect size, and the desired power of the study. Here’s a summary of key points and some references that might help justify your sample size:
Key Points to Consider
  1. Power Analysis:Power analysis is a statistical method used to determine the minimum sample size required for a study to detect an effect of a given size with a given degree of confidence. For correlational studies, a common target for statistical power is 0.80, meaning there's an 80% chance of detecting a true effect. Using Cohen’s guidelines for effect sizes in correlation: small (r = 0.1), medium (r = 0.3), and large (r = 0.5), you can conduct a power analysis to determine the necessary sample size.
  2. Effect Size:The expected effect size directly influences the required sample size. Smaller expected effect sizes necessitate larger sample sizes to detect correlations reliably.
  3. Precedents in Literature:Reviewing similar studies can provide a benchmark for sample size. Many correlational studies in psychology and social sciences use sample sizes ranging from 100 to 300 participants.
  4. Practical Considerations:Practical constraints, such as availability of participants and resources, also play a role in determining the sample size.
Reliable Citations
To provide a robust justification for your sample size, you can refer to the following sources:
  1. Cohen, J. (1992). "A Power Primer." Psychological Bulletin, 112(1), 155-159.This paper provides guidelines for determining sample sizes based on power analysis and effect sizes. Cohen’s guidelines can help you justify why 150 respondents might be adequate for detecting medium to large effect sizes.
  2. Field, A. (2013). "Discovering Statistics Using IBM SPSS Statistics." Sage Publications.Field’s book is a comprehensive resource for understanding statistical methods, including power analysis and sample size determination. It can be used to explain the methodology behind choosing your sample size.
  3. Green, S. B. (1991). "How Many Subjects Does It Take To Do A Regression Analysis?" Multivariate Behavioral Research, 26(3), 499-510.Green discusses sample size requirements for various types of statistical analyses, including correlations. This can help justify your chosen sample size based on similar analyses.
  4. Tabachnick, B. G., & Fidell, L. S. (2019). "Using Multivariate Statistics" (7th ed.). Pearson.This textbook provides detailed guidance on sample size considerations for different statistical techniques. It’s a valuable reference for explaining why 150 respondents can be considered adequate for a correlational study.
  5. Hair, J. F., Black, W. C., Babin, B. J., Anderson, R. E., & Tatham, R. L. (2010). "Multivariate Data Analysis" (7th ed.). Prentice Hall.This text covers a wide range of statistical methods and provides rules of thumb for sample sizes. It can help support your rationale for selecting 150 to 300 participants.
Practical Example
To put it into context, you might say:
"According to Cohen (1992), a medium effect size for correlation (r = 0.3) with a desired power of 0.80 requires a sample size of approximately 84. Therefore, aiming for 150 respondents provides a buffer to ensure reliable detection of medium to large effect sizes. This aligns with precedents in similar studies (Green, 1991; Field, 2013) and adheres to established guidelines for sample size in correlational research (Tabachnick & Fidell, 2019; Hair et al., 2010)."
By referencing these sources, you can robustly justify your sample size of 150 participants for a correlational study.
4o
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Hello,
For my master's thesis I conduct research into the reliability and validity of a questionnaire. I want to determine how many participants I need to include for my study in order that the results will be useful. How can I do the power analysis for a reliability and validity study? Or is there another way to know how many participants I need to incorporate?
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If you require any further clarifications, please feel free to browse through both the e-books attached herewith.
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Hi
Is there any citation to support (consider acceptable) a high value of Cronbach Alpha (CA) and Composite Reliability (CR) (more than 0.95) ?
Additional info: the questionnaire is long but there is no redundant questions.
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Thank you for your sharing Dr Marlon Elías Lobos-Rivera
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  • I've found that some journals are both Scopus-indexed and listed on Beall's list as predatory or potentially predatory. Why does this discrepancy occur?
  • Are there any more reliable platforms than Beall's List for identifying predatory journals?
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Scopus
Scopus is a comprehensive, multidisciplinary database of peer-reviewed literature, including scientific journals, books, and conference proceedings. Launched by Elsevier in 2004, it provides access to a wide array of academic papers and research across various fields. Scopus is known for its extensive coverage and includes features such as citation analysis and author profiles, making it a valuable tool for researchers, academics, and institutions to track and evaluate research output and impact.
Beall's Dilemma
Beall's dilemma refers to the controversy and challenges surrounding predatory publishing, a term popularized by Jeffrey Beall, a librarian and scholar who created Beall's List. This list identified potentially predatory open-access publishers and journals that exploit authors by charging high publication fees without providing the editorial and publishing services associated with legitimate journals. These predatory publishers often lack proper peer review and editorial oversight, leading to concerns about the quality and integrity of the research they publish.
Beall's List was both praised and criticized. Supporters appreciated the efforts to expose unethical publishing practices, while critics argued that the criteria for inclusion were sometimes subjective and that the list could unfairly damage the reputations of legitimate publishers. In 2017, Beall's List was taken offline, but the issue of predatory publishing remains a significant concern in the academic community.
The Connection Between Scopus and Beall's Dilemma
Scopus aims to maintain a high standard for the journals it indexes, but it has faced scrutiny over occasionally including journals from publishers considered predatory or questionable, as identified by Beall's List. This situation creates a dilemma: researchers rely on Scopus for credible sources, yet the presence of potentially predatory journals can undermine trust in the database. Ensuring the quality and reliability of indexed journals while avoiding the pitfalls of predatory publishing is an ongoing challenge for Scopus and other similar databases.
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1. Components of Test Validity (Dimensionality, Item difficulty, Item discrimination, guessing parameter, Carelessness etc
2. Reliability is consistency of outcome in different test occasions.
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In my opinion, test validity is more important than test reliability. I believe that the test should be able to measure the construct before we consider its dependability.
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I am looking for an accurate PLTP activity assay kit (also for CETP and LCAT). Do you have any experience? which company provider is reliable?
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As far as I know the best kits for those analytes is Roar Biomedical
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zzz
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If your solurton is turbid or otherwise does not follow Lambert-Beers law then maybe no. Depends on what you mean by "reliable"
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Any suggestions would be greatly appreciated.
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For microbiota analysis research, it’s crucial to choose a DNA isolation kit that can efficiently and reliably extract high-quality DNA from microbial communities. Here are some cost-effective and reliable DNA isolation kits commonly recommended for this purpose:
1. Qiagen DNeasy PowerSoil Kit
  • Description: This kit is widely used for isolating DNA from complex environmental samples, including soil and fecal samples.
  • Features:Effective at removing inhibitors such as humic acids. High-quality DNA suitable for downstream applications like PCR, qPCR, and next-generation sequencing.
  • Cost: Moderately priced, providing good value for the quality of DNA extracted.
  • Website: Qiagen DNeasy PowerSoil Kit
2. Zymo Research Quick-DNA Fecal/Soil Microbe Miniprep Kit
  • Description: This kit is designed specifically for difficult-to-lyse samples and is efficient at extracting DNA from fecal and soil samples.
  • Features:High-yield and high-purity DNA extraction. Inhibitor removal technology to ensure clean DNA.
  • Cost: Cost-effective, providing reliable results at a reasonable price.
  • Website: Zymo Quick-DNA Fecal/Soil Microbe Miniprep Kit
3. MO BIO PowerSoil DNA Isolation Kit (now part of Qiagen)
  • Description: Known for its efficiency in isolating DNA from soil samples, which are often rich in organic material and inhibitors.
  • Features:Robust protocol for a variety of sample types. Produces high-quality DNA suitable for multiple downstream applications.
  • Cost: Affordable and well-regarded in the research community.
  • Website: MO BIO PowerSoil DNA Isolation Kit
4. Promega Maxwell RSC PureFood GMO and Authentication Kit
  • Description: Although designed for food testing, this kit is adaptable for complex microbiome samples.
  • Features:Automated processing for consistency. High-quality DNA suitable for various genomic applications.
  • Cost: Slightly higher initial investment due to automation, but saves time and labor costs in the long run.
  • Website: Promega Maxwell RSC PureFood GMO and Authentication Kit
5. Norgen Biotek Stool DNA Isolation Kit
  • Description: This kit is optimized for isolating DNA from stool samples, which can be challenging due to the presence of inhibitors.
  • Features:Efficient lysis and inhibitor removal. High-quality DNA ideal for microbiome studies.
  • Cost: Economically priced with good performance metrics.
  • Website: Norgen Biotek Stool DNA Isolation Kit
Recommendations:
  • Sample Type: Choose a kit that is specifically designed for your sample type (e.g., soil, fecal matter).
  • Downstream Applications: Ensure the kit provides DNA quality and purity suitable for your intended downstream applications (e.g., PCR, sequencing).
  • Cost vs. Performance: Consider the balance between cost and the reliability/performance of the kit.
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Which method is more accurate and popular for testing the validity and reliability of my scales?
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Cronbach's alpha is used for reliability, AVE is used for construct validity.
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I'm looking for a way to measure the uncertainty (standard deviation) on the quantification (area) of the components of an XPS spectrum using CasaXPS.
I found these options in the software, but they don't satisfy me:
1) From the "Quantify (F7)" window, in the "Regions" tab, clicking on "Calculate Error Bars" but it is independent of the fit and changes with each click.
2) From the "Quantify (F7)" window, in the "Components" tab, by clicking on "Monte Carlo", I obtain only the relative value and not the absolute one. But above all, the values do not follow the goodness of the fit: even with components that are not fitted and clearly incorrect, the value is low.
As I have not found these methods to be reliable, my idea is to use the RMS as an estimation of the error on the sum of the areas of the components and then obtain the percentage error of the individual components.
My scope is to provide the composition of my sample, and also the percentages of the various components, for each element, all with their measurement error.
Does anyone know if there is a more automatic and reliable method?
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Please watch the videos on this on the CasaXPS YouTube channel. There are several which will aid you in this
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Recently I asked a question related to QCD and in response reliability of QCD itself was challenged by many researchers.
It left me with the question, what exactly is fundamental in physics. Can we rely entirely on the two equations given by Einstien? If not then what can we say as fundamental in physics?
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Are mass energy equation and energy-momentum relation (E^2 = (mc^2)^2 + (pc)^2) fundamental?”
The answer depends on the answer to another question. Do superluminal speeds of matter exist? Recent space exploration shows that such objects exist.
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Is International Journal of Digital Earth a predatory journal or a reliable one?
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Dear Maria Letizia Vitelletti Why do you wonder? It is a well indexed journal https://www.tandfonline.com/action/journalInformation?show=journalMetrics&journalCode=tjde20 with a reliable publisher behind it "Taylor & Francis".
Best regards.
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Hello everyone,
I'm currently working on a project where I need to isolate viruses in culture from incubation with PCR-positive serum samples, but I'm facing the challenge of dissociating antibodies bound to the virus. Does anyone have a reliable protocol or references that could share for pre-treatment of infectious sample for isolation purposes?
Any guidance or suggestions would be greatly appreciated! Thank you in advance for your help.
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Dear Aldo
You can probably do this by lowering the pH using an appropriate buffer over a short period of time, or by a competitive approach via adding recombinant proteins similar to the surface proteins of the virus.
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Actually as I searched about, there are two major method in determining hydrogen peroxide concentration, one is titration with sodium thiosulfate and other is titration with permangenate, I wanted to know which of them are more reliable, and what are the pros and cons of each method.
Thanks
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Determining the concentration of hydrogen peroxide (H2O2) in a sample can be achieved through several methods, depending on the accuracy required and the resources available. Here are some commonly used methods:
1. Titration Method:
Principle: Involves titrating the hydrogen peroxide solution with a standardized solution of a reducing agent, typically potassium permanganate (KMnO4) or potassium dichromate (K2Cr2O7).
Procedure:
  • Preparation: Prepare a standard solution of the oxidizing agent (e.g., KMnO4 or K2Cr2O7) with a known concentration.
  • Titration: Add the standard solution dropwise to the hydrogen peroxide sample until the color changes (indicating the endpoint).
  • Calculation: Use the stoichiometry of the reaction to calculate the concentration of hydrogen peroxide in the sample.
Advantages: Relatively straightforward and can be done with standard laboratory equipment.
Limitations: Requires careful handling of chemicals and precise titration technique.
2. Spectrophotometric Method:
Principle: Measurement of the absorbance of hydrogen peroxide at a specific wavelength using a spectrophotometer.
Procedure:
  • Preparation: Prepare calibration standards of known hydrogen peroxide concentrations.
  • Measurement: Measure the absorbance of each standard and the sample at the appropriate wavelength (typically around 240 nm).
  • Calculation: Construct a calibration curve relating absorbance to concentration. Determine the concentration of hydrogen peroxide in the sample from the curve.
Advantages: High sensitivity and accuracy, suitable for trace analysis.
Limitations: Requires access to a spectrophotometer and preparation of calibration standards.
3. Iodometric Titration Method:
Principle: Involves titrating hydrogen peroxide with iodide ions in the presence of an acid, using a standardized solution of sodium thiosulfate (Na2S2O3) as the titrant.
Procedure:
  • Reaction: H2O2 + 2 I^- + 2 H^+ → 2 H2O + I2
  • Titration: Add Na2S2O3 solution until the iodine color disappears (endpoint).
  • Calculation: Calculate the concentration of H2O2 using the stoichiometry of the reaction.
Advantages: Can be used for both low and high concentrations of hydrogen peroxide.
Limitations: Requires precise pH control and careful handling of reagents.
4. Amperometric Titration Method:
Principle: Measures the current produced by the reduction of hydrogen peroxide at an electrode surface.
Procedure:
  • Electrode Preparation: Use a suitable working electrode (e.g., platinum) and reference electrode.
  • Titration: Add a standardized solution of a reducing agent (e.g., potassium permanganate) to the hydrogen peroxide sample while measuring the current.
  • Calculation: Determine the concentration of hydrogen peroxide based on the amount of reducing agent required to reach the endpoint.
Advantages: Direct and sensitive method suitable for low concentrations.
Limitations: Requires specialized equipment and expertise in electrochemistry.
Considerations:
  • Safety: Hydrogen peroxide is a strong oxidizing agent. Handle with care and follow appropriate safety precautions.
  • Calibration: Always calibrate instruments and prepare standards to ensure accurate measurements.
  • Method Selection: Choose a method based on the concentration range, accuracy needed, and available equipment.
By selecting an appropriate method and following a systematic procedure, you can determine the concentration of hydrogen peroxide in your sample accurately and reliably.
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Other two questions are:
1) Are the conversion tables (grit-microns) found om internet reliable?
2) Where does the equation to convert grit in microns come from?
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Measuring the roughness of sandpaper requires using a method that can quantify the surface texture or irregularities of the abrasive material. Here are several approaches you can consider for measuring the roughness of sandpaper with precision:
1. Surface Profilometer:
  • A surface profilometer is a specialized instrument designed to measure the surface texture of materials. It typically uses a stylus or optical methods to scan the surface and record the profile of irregularities.
  • Procedure: Place the sandpaper sample under the profilometer's stylus or optical sensor. The instrument will then trace the surface, recording parameters such as Ra (average roughness), Rz (maximum peak-to-valley height), and other roughness parameters specified in standards like ISO 4287.
2. Contact Stylus Profilometer:
  • This type of profilometer uses a mechanical stylus to trace the surface roughness. It moves along the sandpaper's surface, measuring deviations and creating a profile.
  • Procedure: Calibrate the stylus profilometer, then scan multiple areas of the sandpaper to obtain an average roughness value (Ra) or other roughness parameters.
3. Non-Contact Optical Profilometer:
  • Optical profilometers use light-based technologies such as confocal microscopy or interferometry to measure surface texture without physically touching the sample.
  • Procedure: Place the sandpaper sample under the optical profiler. The instrument scans the surface with laser or white light and generates a detailed 3D profile, providing roughness parameters with high precision.
4. Atomic Force Microscopy (AFM):
  • AFM is a high-resolution imaging technique that can also measure surface roughness at the nanoscale level.
  • Procedure: Scan the sandpaper surface with the AFM tip, which interacts with the surface at the atomic level, producing a topographical map and roughness analysis.
5. Visual Comparison and Grading Standards:
  • For simpler assessments, visual comparison against standardized roughness samples or grading scales can provide a qualitative measure of sandpaper roughness.
  • Procedure: Use visual aids such as magnification and standardized roughness samples to estimate the level of roughness relative to known standards.
Considerations:
  • Sample Preparation: Ensure the sandpaper sample is flat and securely mounted to avoid movement during measurement.
  • Measurement Standards: Follow applicable standards (e.g., ISO 4287 for surface texture) to ensure consistency and comparability of measurements.
  • Data Analysis: Use software provided with the profilometer to analyze roughness parameters and generate reports.
By employing these methods, you can accurately measure the roughness of sandpaper, providing quantitative data that is crucial for quality control, product development, and ensuring consistency in abrasive performance.
3.5
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In my study, I found an odds ratio (OR) of 65 with a 95% confidence interval (CI) ranging from 1.2 to 195. Given the large odds ratio and the wide confidence interval, what are the potential reasons for these findings? Is this result reliable, and how should it be interpreted?
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You may face a specific problem associated with your dataset and close to perfect separation of the dependent variable based on an independent variable (separation or quasi-separation problem). I invite you to read this intersting post on stack exchange (Very wide confidence intervals for odds ratios - Cross Validated (stackexchange.com)) as well as paper from Irala which gives interesting insights based on a specific example.
To interpret correctly this OR you could use adjusted prediction of your model using for example ggeffect R package.
(ggeffects: Marginal Means And Adjusted Predictions Of Regression Models • ggeffects (strengejacke.github.io)).
I hope this helps!
Irala, J. D., Fernández-Crehuet Navajas, R., & Serrano del Castillo, A. (1997). Abnormally wide confidence intervals in logistic regression: interpretation of statistical program results. Revista Panamericana de Salud Pública, 2, 268-271.
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I would like to look at mitochondrial density in transverse sections of intestine. I do not have access to a TEM. I cannot really determine based on an internet search whether it is feasible to do with light microscopy. Is anyone aware of a reliable method, if one exists. I was contemplating if using a vital dye prior to fixation might be a way to go?
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I think you can stain mitochondries with Janus Green, but I do not know if you can quantify it.
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Dear Colleagues, I need to know how to perform this query for possible answer to reviewer. Thanks and Regards
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Addressing reviewer comments is a crucial part of the manuscript revision process. The reviewer's comment emphasizes the importance of robustness, which demonstrates the reliability of the approach you have used in the paper. To assess reliability, it's important to run experiments with different initializations, hyperparameters, and data splits. Reporting the results will demonstrate the stability of the findings. It's essential to ensure that the model's performance is consistent across different datasets, noise levels, and variations in input data. Additionally, I would suggest conducting a "Sensitivity Analysis" if you haven't done so already. This analysis will provide insights into how changes in hyperparameters affect the model's performance, thereby demonstrating the robustness of the approach.
The reviewer also suggests introducing some originality by highlighting any unique aspects of the approach. You should highlight any unique aspects of your approach. Emphasize the original aspects and clearly state the contributions of your work. What novel insights or improvements does your proposed method/approach offer? Make sure to clearly explain how your work or approach is different from previous works in the field.
Good Luck..!
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Dear ResearchGate Community,
I hope this message finds you well.
As an active researcher, I am always looking to participate in reputable academic conferences to present my work and collaborate with fellow scholars. However, I have become increasingly concerned about the proliferation of predatory conferences, which lack academic rigor and exploit researchers.
Could you please recommend reliable websites or databases where I can find information about credible academic conferences (especially in Management, and Psychology)?
Your guidance and any shared experiences would be greatly appreciated.
Thank you for your assistance.
Best regards,
Jyun-Kai  Liang,
Associate Professor, Department of Applied Psychology, Hsuan Chuang University
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Hi,
Follow Beal's list fir predatory journals and organizations. Also, see who is organizing and do a background search in internet and you can easily find out if that was predatory.
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What sample size would be most suitable for a structural equation modeling study to achieve reliable and generalizable results?
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Heba Ramadan Thank you so much for sharing this insightful summary of the SEM models! This perspective will undoubtedly help me better understand and interpret the results of my own SEM analyses.
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Dear network, I am looking for a reliable and fast lab for geochemical analyzes for bulk rock (major and trace elements).
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Hello Jeanne, Actlabs is a good choice in my opinion (https://actlabs.com/)
Saludos,
Marco
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I am conducting research for my capstone project on the accuracy and completeness of ChatGPT-generated medical information and would greatly appreciate your insights and expertise on this topic.
Below are a few questions I have regarding the methodology used in assessing ChatGPT-generated medical information but feel free to offer any alter ate insights.
1. What methodologies are commonly employed to evaluate the accuracy and completeness of AI-generated medical responses like those produced by ChatGPT?
2. Could you provide examples of specific metrics or criteria used to assess the accuracy of ChatGPT-generated medical information?
3. How do researchers ensure the reliability of human assessments when grading the accuracy and completeness of ChatGPT-generated medical responses?
4. Are there any established guidelines or best practices for designing experiments to evaluate the performance of ChatGPT in generating medical information?
5. In your experience, what are the main challenges or limitations associated with current methodologies used to assess the accuracy and completeness of ChatGPT-generated medical information?
Your valuable input will greatly contribute to the depth and rigor of my research. Thank you in advance for your time and consideration.
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You should verify the medical information provided by ChatGPT with trusted medical professionals or authoritative sources. While ChatGPT strives for accuracy, it is not a substitute for professional medical advice or diagnosis. You should exercise caution and critical thinking when interpreting and acting upon the information provided by ChatGPT.
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Hello everyone
I am analyzing data, and one of the instruments was used to measure the level of diabetes knowledge. The instrument is a quiz, and it has a total score. How can I measure the reliability of this quiz format instrument? How can I measure the consistency of the instrument?
Any related discussion or information would be appreciated.
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Thank you so much for the insightful reply.
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This is my first experience, getting an invitation to participate in writing a book chapter at intechopen.
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The article "Validity and Reliability of Measurement Instruments Used in Research" by Carole L. Kimberlin and Almut G. Winterstein emphasizes the importance of reliability and validity in research instruments for accurate and consistent data collection. It emphasizes the development and validation process, reliability estimates, validity, responsiveness to change, and data accuracy, particularly in healthcare and social science research, where accurate and reliable instruments are crucial for quality research.
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Dear Steven Cooke, I'm writing to thank you for taking the time to respond so thoroughly and informatively to my inquiries about measuring reliability, validity, and accuracy in the social sciences. It is clear that your expertise in this field has allowed you to convey a thorough comprehension of these complex issues.
I look forward to incorporating this newfound knowledge into my own research pursuits.
Warm regards,
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If you design a scale specimen to do a seismic experiment. What should you look for to make the experiment reliable?
1.In microscale experiments should you do everything in microscale and the displacement between two points?
2.The models must have the scale within their structure, so that the sub-scale intensity of the earthquake causes corresponding sub-scale displacements that agree with elastic theory ?
If anyone knows the rules of microscale seismic experiments it would be very useful for me to know them. Thanks a lot!
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I did this experiment on a microscale of one to seven.
Base oscillation width 15 cm, 30cm go and 30cm come Full travel 60cm with a frequency of 2Hz and sometimes it reached 3 Hz. At 2Hz the natural acceleration of the experiment was measured at 2.41g
The question is the 2.41g of the experiment under scale, how much physical acceleration does it correspond to?
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Hi dear researchers!
Could you please tell me what are the main reliable journals with articles that are talking about groundwater quality, isotopic analysis and salinity?
Thank you in advance
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Thank you so much for your help!
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Can we stop global climate change? Does human scientific power reach the world's climate change? How do researchers respond?
As you know, humans are very intelligent and can predict the future climate of the world with hydrology, climatology and paleontology. But don't countries, especially industrialized countries, that produce the most harmful gases in the earth's atmosphere and think about the future of the earth's atmosphere? Do they listen to the research of climatologists? What would have to happen to force them to listen to climate scientists?
Miloud Chakit added a reply
Climate change is an important and complex global challenge, and scientific theories about it are based on extensive research and evidence. The future path of the world depends on various factors including human actions, political decisions and international cooperation.
Efforts to mitigate and adapt to climate change continue. While complete reversal may be challenging, important steps can be taken to slow progression and lessen its effects. This requires global cooperation, sustainable practices and the development and implementation of clean energy technologies.
Human scientific abilities play an important role, but dealing with climate change also requires social, economic and political changes. The goal is to limit global warming and its associated impacts, and collective action at the local, national, and international levels is essential for a more sustainable future.
Reply to this discussion
Osama Bahnas added a reply
It is impossible to stop global climate change. The human scientific power can not reach the world's climate change.
Borys Kapochkin added a reply
Mathematical models of increasing planetary temperature as a function of the argument - anthropogenic influence - are erroneous.
Alastair Bain McDonald added a reply
We could stop climate change but we won't! We have the scientific knowldge but not the political will. One could blame Russia and China from refusing to cooperate but half the population of the USA (Republicans) deny climate change is a problem and prefer their profligate life styles reply:
All climate change has been loaded on the CO2 responsible for the greenhouse effect. Therefore, there must be scientific experiments from several independent scientific institutes worldwide to find out what the greenhouse impact is on various CO2 concentrations. Then, there must be a conference from a reliable, professional organization with the participation of all independent scientific institutions to establish standards on CO2 concentrations and propose political actions accordingly.
The second action that can be done is to plant as many trees and plants as possible to breathe the CO2 and free the oxygen. Stop any deforestation and plant trees immediately in any bunt areas.
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Effect of Injecting Hydrogen Peroxide into Heavy Clay Loam Soil on Plant Water Status, NET CO2 Assimilation, Biomass, and Vascular Anatomy of Avocado Trees
In Chile, avocado (Persea americana Mill.) orchards are often located in poorly drained, low-oxygen soils, situation which limits fruit production and quality. The objective of this study was to evaluate the effect of injecting soil with hydrogen peroxide (H2O2) as a source of molecular oxygen, on plant water status, net CO2 assimilation, biomass and anatomy of avocado trees set in clay loam soil with water content maintained at field capacity. Three-year-old ‘Hass’ avocado trees were planted outdoors in containers filled with heavy loam clay soil with moisture content sustained at field capacity. Plants were divided into two treatments, (a) H2O2 injected into the soil through subsurface drip irrigation and (b) soil with no H2O2 added (control). Stem and root vascular anatomical characteristics were determined for plants in each treatment in addition to physical soil characteristics, net CO2 assimilation (A), transpiration (T), stomatal conductance (gs), stem water potential (SWP), shoot and root biomass, water use efficiency (plant biomass per water applied [WUEb]). Injecting H2O2 into the soil significantly increased the biomass of the aerial portions of the plant and WUEb, but had no significant effect on measured A, T, gs, or SWP. Xylem vessel diameter and xylem/phloem ratio tended to be greater for trees in soil injected with H2O2 than for controls. The increased biomass of the aerial portions of plants in treated soil indicates that injecting H2O2 into heavy loam clay soils may be a useful management tool in poorly aerated soil.
Shade trees reduce building energy use and CO2 emissions from power plants
Urban shade trees offer significant benefits in reducing building air-conditioning demand and improving urban air quality by reducing smog. The savings associated with these benefits vary by climate region and can be up to $200 per tree. The cost of planting trees and maintaining them can vary from $10 to $500 per tree. Tree-planting programs can be designed to have lower costs so that they offer potential savings to communities that plant trees. Our calculations suggest that urban trees play a major role in sequestering CO2 and thereby delay global warming. We estimate that a tree planted in Los Angeles avoids the combustion of 18 kg of carbon annually, even though it sequesters only 4.5-11 kg (as it would if growing in a forest). In this sense, one shade tree in Los Angeles is equivalent to three to five forest trees. In a recent analysis for Baton Rouge, Sacramento, and Salt Lake City, we estimated that planting an average of four shade trees per house (each with a top view cross section of 50 m2) would lead to an annual reduction in carbon emissions from power plants of 16,000, 41,000, and 9000 t, respectively (the per-tree reduction in carbon emissions is about 10-11 kg per year). These reductions only account for the direct reduction in the net cooling- and heating-energy use of buildings. Once the impact of the community cooling is included, these savings are increased by at least 25%.
Can Moisture-Indicating Understory Plants Be Used to Predict Survivorship of Large Lodgepole Pine Trees During Severe Outbreaks of Mountain Pine Beetle?
Why do some mature lodgepole pines survive mountain pine beetle outbreaks while most are killed? Here we test the hypothesis that mature trees growing in sites with vascular plant indicators of high relative soil moisture are more likely to survive mountain pine beetle outbreaks than mature trees associated with indicators of lower relative soil moisture. Working in the Clearwater Valley of south central British Columbia, we inventoried understory plants growing near large-diameter and small-diameter survivors and nonsurvivors of a mountain pine beetle outbreak in the mid-2000s. When key understory species were ranked according to their accepted soil moisture indicator value, a significant positive correlation was found between survivorship in large-diameter pine and inferred relative high soil moisture status—a finding consistent with the well-documented importance of soil moisture in the mobilization of defense compounds in lodgepole pine. We suggest that indicators of soil moisture may be useful in predicting the survival of large pine trees in future pine beetle outbreaks. Study Implications: A recent outbreak of the mountain pine beetle resulted in unprecedented levels of lodgepole pine mortality across southern inland British Columbia. Here, we use moisture-dependent understory plants to show that large lodgepole pine trees growing in sites with high relative moisture are more likely than similar trees in drier sites to survive severe outbreaks of mountain pine beetle—a finding that may be related to a superior ability to mobilize chemical defense compounds compared with drought-stressed trees.
Can Functional Traits Explain Plant Coexistence? A Case Study with Tropical Lianas and Trees
Organisms are adapted to their environment through a suite of anatomical, morphological, and physiological traits. These functional traits are commonly thought to determine an organism’s tolerance to environmental conditions. However, the differences in functional traits among co-occurring species, and whether trait differences mediate competition and coexistence is still poorly understood. Here we review studies comparing functional traits in two co-occurring tropical woody plant guilds, lianas and trees, to understand whether competing plant guilds differ in functional traits and how these differences may help to explain tropical woody plant coexistence. We examined 36 separate studies that compared a total of 140 different functional traits of co-occurring lianas and trees. We conducted a meta-analysis for ten of these functional traits, those that were present in at least five studies. We found that the mean trait value between lianas and trees differed significantly in four of the ten functional traits. Lianas differed from trees mainly in functional traits related to a faster resource acquisition life history strategy. However, the lack of difference in the remaining six functional traits indicates that lianas are not restricted to the fast end of the plant life–history continuum. Differences in functional traits between lianas and trees suggest these plant guilds may coexist in tropical forests by specializing in different life–history strategies, but there is still a significant overlap in the life–history strategies between these two competing guilds.
The use of operator action event trees to improve plant-specific emergency operating procedures
Even with plant standardization and generic emergency procedure guidelines (EPGs), there are sufficient dissimilarities in nuclear power plants that implementation of the guidelines at each plant must be performed in a manner that ensures consideration of plant-specific design features and operating characteristics. The use of operator action event tress (OAETs) results in identification of key features unique to each plant and yields insights into accident prevention and mitigation that can be factored into plant-specific emergency procedures. Operator action event trees were developed as a logical extension of the event trees developed during probabilistic risk analyses. The dominant accident sequences developed from a plant-specific probabilistic risk assessment represent the utility's best understanding of the most likely combination of events that must occur to create a situation in which core cooling is threatened or significant releases occur. It is desirable that emergency operating procedures (EOPs) provide adequate guidance leading to appropriate operator actions for these sequences. The OAETs provide a structured approach for assuring that the EOPs address these situations.
Plant and Wood Area Index of Solitary Trees for Urban Contexts in Nordic Cities
Background: We present the plant area index (PAI) measurements taken for 63 deciduous broadleaved tree species and 1 deciduous conifer tree species suitable for urban areas in Nordic cities. The aim was to evaluate PAI and wood area index (WAI) of solitary-grown broadleaved tree species and cultivars of the same age in order to present a data resource of individual tree characteristics viewed in summer (PAI) and in winter (WAI). Methods: All trees were planted as individuals in 2001 at the Hørsholm Arboretum in Denmark. The field method included a Digital Plant Canopy Imager where each scan and contrast values were set to consistent values. Results: The results illustrate that solitary trees differ widely in their WAI and PAI and reflect the integrated effects of leaf material and the woody component of tree crowns. The indications also show highly significant (P < 0.001) differences between species and genotypes. The WAI had an overall mean of 0.91 (± 0.03), ranging from Tilia platyphyllos ‘Orebro’ with a WAI of 0.32 (± 0.04) to Carpinus betulus ‘Fastigiata’ with a WAI of 1.94 (± 0.09). The lowest mean PAI in the dataset was Fraxinus angustifolia ‘Raywood’ with a PAI of 1.93 (± 0.05), whereas Acer campestre ‘Kuglennar’ represents the cultivar with the largest PAI of 8.15 (± 0.14). Conclusions: Understanding how this variation in crown architectural structure changes over the year can be applied to climate responsive design and microclimate modeling where plant and wood area index of solitary-grown trees in urban contexts are of interest.
Do Exotic Trees Threaten Southern Arid Areas of Tunisia? A Case Study Indian Journal of Ecology (2020) 00(0): 000-000 Plant-plant interactions
an afforested steppe planted This study was conducted in with aims to compare the effects of exotic and native Stipa tenacissima trees (and , respectively) on the understory vegetation and soil properties. For each tree species, two sub-Acacia salicina Pinus halepensis habitats were distinguished: the canopied sub-habitat (under the tree crown) and the un-canopied sub-habitat (open grassland). Soil moisture was measured in both sub-habitats at 10 cm depth. In parallel to soil moisture, investigated the effect of tree species on soil fertility. Soil samples were collected from the upper 10 cm soil, excluding litter and stones. The nutrient status of soil (organic matter, total N, extractable P) was significantly higher under compared to and open areas. This tendency remained constant with the soil water A. salicina P. halepensis content which was significantly higher under trees compared to open sub-habitats. For water content, there were no significant differences between studied trees. Total plant cover, species richness and the density of perennial species were significantly higher under the exotic species compared to other sub-habitats. Among the two tree species, had the strongest positive effect on the understory Acacia salicina vegetation. It seems to be more useful as a restoration tool in arid areas and more suitable to create islands of resources and foster succession than the other investigated tree species.
Effects of Elevated Atmospheric CO2 on Microbial Community Structure at the Plant-Soil Interface of Young Beech Trees (Fagus sylvatica L.) Grown at Two Sites with Contrasting Climatic Conditions
Soil microbial community responses to elevated atmospheric CO2 concentrations (eCO2) occur mainly indirectly via CO2-induced plant growth stimulation leading to quantitative as well as qualitative changes in rhizodeposition and plant litter. In order to gain insight into short-term, site-specific effects of eCO2 on the microbial community structure at the plant-soil interface, young beech trees (Fagus sylvatica L.) from two opposing mountainous slopes with contrasting climatic conditions were incubated under ambient (360 ppm) CO2 concentrations in a greenhouse. One week before harvest, half of the trees were incubated for 2 days under eCO2 (1,100 ppm) conditions. Shifts in the microbial community structure in the adhering soil as well as in the root rhizosphere complex (RRC) were investigated via TRFLP and 454 pyrosequencing based on 16S ribosomal RNA (rRNA) genes. Multivariate analysis of the community profiles showed clear changes of microbial community structure between plants grown under ambient and elevated CO2 mainly in RRC. Both TRFLP and 454 pyrosequencing showed a significant decrease in the microbial diversity and evenness as a response of CO2 enrichment. While Alphaproteobacteria dominated by Rhizobiales decreased at eCO2, Betaproteobacteria, mainly Burkholderiales, remained unaffected. In contrast, Gammaproteobacteria and Deltaproteobacteria, predominated by Pseudomonadales and Myxococcales, respectively, increased at eCO2. Members of the order Actinomycetales increased, whereas within the phylum Acidobacteria subgroup Gp1 decreased, and the subgroups Gp4 and Gp6 increased under atmospheric CO2 enrichment. Moreover, Planctomycetes and Firmicutes, mainly members of Bacilli, increased under eCO2. Overall, the effect intensity of eCO2 on soil microbial communities was dependent on the distance to the roots. This effect was consistent for all trees under investigation; a site-specific effect of eCO2 in response to the origin of the trees was not observed.
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Michael Senteza added a reply:
We have to separate science from business and politics in the first place , before we can adequately discuss the resolution of this global challenge .
The considerations to global warming can be logically broken down in the following
1. What are the factors that have affected the earths climate over the last million years ? 100,000 years , 10,000 years and 1,000 years .
2. Observations , the climatic changes , formations , and archaeological data to support the changes
3. The actualities of the earth dynamics , for example we know that approx 2/3 of the earth is water and we also know that of the 1/3 we have approximately 60% un inhabitable , and the 40% habitable has approximately 10% who contribute to the alleged pollution , where for example as of 2022 (https://www.whichcar.com.au/news/how-many-cars-are-there-in-the-world) The US had 290 Million cars compared to Africa (50+ Countries ) 26 Million cars the EU (33 + countries ) 413 million cars then Asia pacific with 543 Million cars ( with a population of close to 2 billion ) . We estimate that as of may there are 1.45 billion cars . this means that North America , Western Europe and Asia pacific combined have approx 1.3 billion cars , and yet close to 70% of vegetation cover and forest space is concentrated in africa , south america , northern europe and canada. we need to analyse this
4. We need to also analyse the actualities of the cause separating factors outside our reach , for example global worming as opposed to climate change . We know that climate change which has been geologically and scientifically observed to have been the reason things like Oil came into place , species became extinct and other formations created . We need to realise that a fair share of changes in climate (which some times may be confused with global worming ) have been due to changes in the earth's rotation , axis and orbit around the sun . These are factors that greatly affect the distribution of the sun's radiation on to the surface of the earth and the atmospheric impact , them make consideration of how much we produce , the dispersion rate , natural chemical balances and volumetric analysis of concentration , assimilation and alteration of elements .
5. The extent to which non scientific factors are contributing to attenuating strength of scientific argument . It is not uncommon to have politicians alter the rhetoric to serve their agenda , however it's even worse when the sponsors of the scientific research are intent on achieving specific goals and not facts .
In conclusion humans are intelligent enough to either end of mitigation the impact of global worming if it can be detached from capitalism and politics . Science can and will provide answers
Sunil Deshpande added a reply:
World‘s scientific power is doing its best to stop the global climate change. For example , alternatives to Petrol, cement, plastic are already identified and once they are consumed by many, it will have a positive impact to stop the climate change. However, to my mind, its not sufficient unless citizen of every country also contribute in his own way to stop climate change such as stopping the use of plastic, use of electric car against Petrol, stopping the engine of car at traffic signal. It should become a global movement to protect the climate.
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Greetings and politeness and respect. Thank you very much and thank you very much.
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In my research, I have 11 multiple-choice questions about environmental knowledge, each question with one correct option, three incorrect options, and one "I don't know" option (5 options in total). When I coded my data into SPSS (1 for correct and 0 for incorrect responses) and ran a reliability analysis (Cronbach's Alpha), it was around 0,330. I also ran a KR20 analysis since the data is dichotomous but still not over 0,70.
These eleven questions have been used in previous research, and when I checked them, they all stated a reliability over 0,80 with a similar sampling to the sampling of my research. This got me thinking whether I was doing something wrong.
Low reliability might be caused by each question measuring knowledge from different environmental topics? If this is the case, do I still have to state its reliability when using the results in my study? For example, I can give correct and incorrect response percentages, calculate the sum points, etc.
Thank you!
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If the questions tap into different topics (i.e., in case of multidimensionality), it likely does not make sense to apply a reliability measure such as Cronbach's alpha. Alpha implies a unidimensional scale/measurement model (i.e., all items measuring a single common factor).
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H-INDEX & CITATION EVALUATIONS OF ACADEMICIANS, HOW MUCH RELIABLE !?
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The opinion that the first author always did the most for the results and for writing an article is not correct. Sometimes the leader of a group of authors is at the first place, sometimes all authors are given in alphabetic order.
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please suggest me a reliable protocol
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There are good published protocols that you will find through Google or Google Scholar
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These terms are all related to the evaluation of research instruments, particularly surveys and questionnaires. Here's a breakdown of each:
Cronbach's alpha (α): This is a widely used statistic to assess the internal consistency or reliability of a test or scale. It essentially measures how closely related the items in your survey are to each other. High Cronbach's alpha indicates that the items are all measuring the same underlying concept and provide consistent results.
Average Variance Extracted (AVE): This statistic is used to assess the convergent validity of a construct in a structural equation model (SEM). Convergent validity shows how well the indicators (survey questions) represent the underlying construct they are intended to measure. A high AVE value suggests that a greater proportion of the variance in the indicators is due to the intended construct, rather than measurement error.
Composite Reliability (CR): Similar to Cronbach's alpha, CR is another measure of internal consistency in the context of SEM. It reflects the reliability of the composite score derived from multiple indicators. A high CR value indicates that the composite score is a reliable estimate of the underlying construct.
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Research instruments are essentially the tools researchers use to gather information and data for their studies. They can be broadly categorized into two main types:
Data Collection Instruments: These tools are used to specifically collect new data directly related to the research question.
Examples include:
Surveys and questionnaires: These involve asking participants a set of questions to gather their opinions, experiences, or behaviors.
Interviews: One-on-one or group discussions where the researcher asks questions and gathers detailed qualitative data.
Observations: Researchers directly observe subjects or phenomena of interest, taking detailed notes or recordings.
Focus groups: A small group discussion guided by a researcher to explore specific topics or gain insights into group dynamics.
Data Analysis Instruments: These tools help researchers organize, analyze, and interpret the data they've collected.
Examples include:
Statistical software: Used to analyze quantitative data, identify patterns, and test hypotheses.
Coding software: Helps researchers categorize and analyze qualitative data from interviews, observations, or open-ended survey responses.
All research instruments are interrelated in the sense that they work together to achieve the research goals. The choice of instrument depends on the type of data needed (quantitative or qualitative) and the research question being asked.
Here's how they connect:
Researchers use data collection instruments to gather the raw information needed for their study.
This data is then fed into data analysis instruments that help make sense of it, identify patterns, and draw conclusions.
The effectiveness of the research ultimately depends on the quality of both the data collection and analysis instruments used.
By choosing the right research instruments and using them effectively, researchers can ensure they gather reliable and valid data to answer their research questions.
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Certainly! Here's a refined version of your question:
"I've been encountering challenges downloading and reading journal articles. Despite some being accessible through Sci-Hub using their DOI numbers, there are still many that remain unavailable. Is there any reliable solution to overcome this issue?"
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There are several websites and platforms where you can download articles easily, depending on your specific needs and the type of articles you're looking for. Here are a few options:
  1. Google Scholar: Google Scholar is a freely accessible web search engine that indexes the full text or metadata of scholarly literature across an array of publishing formats and disciplines. It often provides direct links to PDFs of articles.
  2. PubMed: PubMed is a free search engine accessing primarily the MEDLINE database of references and abstracts on life sciences and biomedical topics. Many articles on PubMed are available for free, and you can easily download them in PDF format.
  3. ResearchGate: ResearchGate is a social networking site for scientists and researchers to share papers, ask and answer questions, and find collaborators. Many researchers upload their articles on ResearchGate, making it a good resource for accessing scholarly literature.
  4. Sci-Hub: Sci-Hub is a website that provides free access to millions of research papers and books by bypassing paywalls. However, the legality of using Sci-Hub varies by jurisdiction, so use it at your own discretion.
  5. Academia.edu: Academia.edu is a platform for academics to share research papers. While not all articles on Academia.edu are freely accessible, many authors choose to share their work publicly, making it a useful resource for finding and downloading articles.
  6. Library Databases: Many academic libraries provide access to a wide range of subscription-based databases, such as JSTOR, IEEE Xplore, and ScienceDirect, which offer access to scholarly articles in various disciplines. If you're affiliated with a university or research institution, you likely have access to these databases through your library's website.
Before downloading articles from any website, make sure to check the copyright status and terms of use to ensure compliance with copyright laws and publisher policies. Additionally, be cautious when using platforms like Sci-Hub, as they may operate in legal gray areas.
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The Sysmex DI-60 is a machine found in labs that counts blood cells and checks other blood details. It helps doctors diagnose and keep track of health conditions by giving accurate results
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The Sysmex DI-60 is a hematology analyzer used for the analysis of blood samples. It employs several methodologies to analyze blood samples, each contributing to its accuracy and reliability:
  1. Impedance Method: This method measures changes in electrical impedance as blood cells pass through an aperture. By analyzing the magnitude and shape of the impedance pulses, the analyzer can differentiate between different types of blood cells (e.g., red blood cells, white blood cells, and platelets). The impedance method is particularly useful for cell counting and sizing.
  2. Optical Scatter Method: This method involves shining a laser or light source onto blood cells and analyzing the scattered light patterns. Different cell types scatter light in characteristic ways, allowing the analyzer to identify and quantify them. The optical scatter method is effective for distinguishing between various cell types based on their morphology and internal structure.
  3. Fluorescence Flow Cytometry: This method utilizes fluorescent dyes that selectively bind to specific cellular components, such as DNA, RNA, or surface markers. By measuring the fluorescence emitted by stained cells as they pass through a flow cell, the analyzer can provide information about cell populations, cellular function, and abnormal cell characteristics.
  4. Hemoglobinometry: This method measures the concentration of hemoglobin in blood samples, typically using cyanide-free reagents that react with hemoglobin to produce a colorimetric change. By quantifying the amount of light absorbed or transmitted by the hemoglobin solution, the analyzer can determine hemoglobin levels accurately.
These methodologies work synergistically to provide comprehensive information about blood cell counts, morphology, and hemoglobin levels. By combining multiple analytical techniques, the Sysmex DI-60 can enhance the accuracy, reliability, and efficiency of blood sample analysis, leading to more precise diagnostic results and improved patient care. Additionally, advanced algorithms and quality control mechanisms further contribute to the analyzer's performance by minimizing errors and ensuring consistency in results.
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Prior to the research carried out by the research group who published this journal, they assessed HDV RNA detection's diagnostic efficacy utilizing the HDV RNA detection technique and discovered that it had a good diagnostic yield. However, HDV serology's diagnostic effectiveness hasn't been thoroughly assessed and documented, thus, the need to consider the factors needed in developing a reliable serological test for HDV antibodies.
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Developing a reliable serological test for HDV antibodies that can effectively account for diverse genotypes requires careful consideration of several factors:
  1. Antigen Selection: Identifying antigens that are conserved across different HDV genotypes is essential to ensure the test's effectiveness across various strains. Antigenic regions that are highly conserved among different genotypes would be preferable for maximizing test sensitivity.
  2. Cross-Reactivity: Assessing potential cross-reactivity with antibodies from other related viruses, such as hepatitis B virus (HBV), is crucial to avoid false-positive results. HDV commonly infects individuals with HBV, so distinguishing between antibodies to HDV and HBV is important for accurate diagnosis.
  3. Genotype-Specific Detection: Developing assays capable of detecting genotype-specific antibodies can provide valuable information about the specific HDV strain present in an individual. This may involve incorporating genotype-specific antigens or optimizing assay conditions to enhance genotype discrimination.
  4. Sensitivity and Specificity Optimization: Ensuring high sensitivity to detect even low levels of antibodies and high specificity to minimize false-positive results is critical for the reliability of the test. This may involve optimizing assay parameters, such as antigen concentration, incubation time, and detection methods.
  5. Validation with Diverse Patient Samples: Testing the serological assay with a diverse range of patient samples, including those infected with different HDV genotypes and individuals with co-infections (e.g., HBV/HDV), is necessary to validate its effectiveness across various populations.
  6. Quality Control Measures: Implementing robust quality control measures throughout the development and manufacturing process is essential to ensure the consistency and reproducibility of the test results.
By carefully considering these factors, researchers can develop a serological test for HDV antibodies that effectively accounts for the diversity of HDV genotypes and provides accurate diagnostic information. This can significantly improve our ability to diagnose and manage HDV infections in clinical settings.
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This question is based on this article:
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According to the evaluation performance of Sysmex DI-60 by Kweon, et al (2022), in comparison to manual slide inspection, DI-60 recognized polychromasia, target cells, and ovalocytes with satisfactory accuracy. However, DI-60 had low specificity (10.4%) for schistocytes despite its great sensitivity (97.2%). In the precision analysis of RBC morphological characterization, borderline samples containing specific RBCs revealed variations in the positive results over 20 replicates. In particular, six out of ten samples showed discrepancies in schistocyte precision. For WBC differentials, the overall agreement between pre-classification results and user-verified results was 89.4%. Except for basophils, normal WBCs had a strong connection with DI-60 (after user verification) and manual counts.
In conclusion, DI-60 performed satisfactorily with normal samples. However, it should be used cautiously, though, for aberrant WBC differentials and RBC morphological characterization. But it is also important to take note that DI-60 has the potential to enhance laboratory productivity while increasing viability.
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Although Sysmex DI-60 is a fully automated analyzer, further understanding and analytical processes to ensure the reliability of its functionality are needed concerning the factors and their impact on its sample quality or preparation.
With this, what is the impact of sample quality or preparation variations on the accuracy and consistency of red blood cell morphology characterization by the DI-60 analyzer?
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The impact of sample quality or preparation variations on the accuracy and consistency of red blood cell morphology characterization by the DI-60 analyzer include (Oh Joo Kweon et al., 2022):
1. Blood Smear Quality - The quality of the blood smear, thickness, uniformity, and staining intensity, can influence the ability of DI-60 to accurately identify and classify red blood cell morphologies. Smears that are too thick or thin, unevenly spread, or inadequately stained may lead to misinterpretation of its morphology.
2. Cell Distribution: The distribution of red blood cells on the smear, such as clumping or aggregation of cells, can impact the ability of the DI-60 analyzer to locate and analyze individual cells. Clumped cells may be incorrectly identified or missed, affecting the accuracy of morphology characterization.
3. Cell Integrity: Integrity of RBCs in the sample, including factors like cell shape abnormalities, cell lysis, or presence of artifacts, can affect the analyzer's ability to accurately classify cell morphologies. Damaged or distorted cells may be challenging for the analyzer to categorize correctly.
4. Staining Artifacts: Variations in staining techniques or the presence of staining artifacts on the smear can interfere with the analyzer's ability to differentiate between different red blood cell morphologies. Staining artifacts may lead to false-positive or false-negative results in morphology characterization.
5. User Proficiency: Proficiency of the user in preparing blood smears and operating the DI-60 analyzer can also impact the analysis. Proper training and adherence to standardized protocols are essential to ensure reliable results.
Further research is needed to systematically evaluate the influence of sample quality and preparation variations on the performance of the DI-60 analyzer in red blood cell morphology characterization. By identifying and addressing potential sources of variability, laboratories can optimize the accuracy and consistency of results obtained with automated cell imaging analyzers like the DI-60.
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We would like to buy a new cell counting device for our Lab, and we're searching for a device, with a reasonable price. However, we're not sure about the reliability and the reproducibility of the results using different devices, including Logos LUNA II, Thermofisher Countess II, Biorad TC20, etc.
personally, I've used LUNA II with their 2-chamber slides and I was happy with the repeatability. Also I've used Countess II with reusable slides which was not a satisfactory experience. But I can't make a decision between the two as they were not both with single-use chamber slides.
I would appreciate if anyone can provide a head-to-head comparison, or device-to-hemocytometer comparison, or at least share their device reliability experience?
Many thanks.
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Hello Ali Babaie ,
Although you probably already decided on a cell counter here is a paper comparing the results of a hemocytometer with an automated cell counter:
However choosing the right cell counter for you always depends on your needs. Reliability, Throughput, Cell type, concentration matter in the decision.
Most companies offer Application notes on their websites stating the accuracy of their devices.
Comparing customer experiences on e.g. Select Science might also help.
But the best way to find the right fit is to try them out in a demonstration.
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Machine learning can be used for prediction of antibiotic resistance in healthcare setting. The problem is that most hospitals do not have electronic health records. Datasets need to be large to make models reliable. I need a dataset with minimum of 1000 records. Kindly assist
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I would approach the pathology laboratory attached to the hospital group amd speak to the microbiologist or speak to the occupational infection control head at the facility that does the surveillance for the hospital on the antibiograms for the facility
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Hello,
I'm seeking recommendations for safe and reliable software to anonymize CT scan images for research.
While DICOMCleaner has been our go-to for removing DOB, names, and other PHI, it's become outdated and prone to frequent crashes. Your suggestions would be greatly appreciated.
Thank you in advance.
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Are there investors on the forum?
The theory of the Genesis of earthquakes has been created.
Based on this theory, the main fields, the main forces involved in the development of earthquakes, are determined.