Real Time - Science topic

when I had Put the pcr tubes in the thermocycler for cDNA synthesis, there was a power breakage of 5 minutes after which the reaction resumed. I performed the nanodrop which gave satisfactour results, the comcentration and purity of the cDNA were both good. But after performing real-time pcr, I’m not getting results. should I synthesise the cDNA again too or should I use the previously synthesised cDNA (used in this reaction) for real-time again?

Hello. If their first cDNA concentrations were equal for each sample, your second sample has a lower amount of final amplification. It depends on the efficiency of your primer and its TM. Did you optimize your primers? And what was the gene? I had seen that mutations could influence primer annealing and melting curve

Hi

Yes, I also agree you need to optimize the reactions first, what ever polymerase you are using. Also the nature of enzyme (hot start/regular) will also make difference.

The HIL setups allow us to test a real piece of hardware (hence the name - hardware-in-the-loop) with its pertaining features and operational modes.

Specifically, in a controller-hardware-in-the-loop (C-HIL, just one type of HIL setup) setup we can test how the controller with an embedded code is going to behave once deployed in an actual device. What is hard to model or do in e.g. MATLAB in this regard is the following:

- Controller capacities utilization - in various forms! (memory overrun, interrupts execution, etc.),

- Coordination and execution of different control layers (especially if they are executed on different controllers/control platforms),

- Communication devices and systems, overlaying the power electronics system (how are you going to model dynamics associated with the CAN communication protocol, for example, used to exchange the references between the controllers?),

- Integration with other real-time executed tools (for example, flight simulator for testing more electric airplanes),

- etc.

So to abstract things - Doing tests on a certain piece of hardware in real-time allows us to connect these devices to other devices and processes that are also executed in real-time (and sometimes can not be easily executed in non-real-time context) and to examine many features that are simply hard to model, or sometimes meaningless, in an offline simulation.

On the other hand, and more broadly speaking, unlike MATLAB, the HIL setups allow us to model and test:

- Medium/large complexity and size systems (for example, microgrids are extremely hard to model in detail since the model runtime tends to increase exponentially),

- High-power/high-voltage devices (in a power-hardware-in-the-loop, P-HIL, setup we can test the behavior of the real protection device in various network situations, such as short-circuits, loss of phase, etc. - this is next to impossible to do in offline simulation),

- etc.

This all being said, the offline simulation tools (constituting model-in-the-loop, MIL, setup) are here to stay. They are still great for initial tests, to put it in simple terms, and simulation tool providers are constantly adding new useful features, but the HIL setups have closed the serious gap that existed between the offline simulations and the tests on actual devices (prototypes or commercially available devices). It is safer than the real hardware, but faster and 'more precise' (again serious simplification) than the models executed in offline simulations.

You can check the following two documents for further details if you wish.

Do you mean that you blast the primers and probe sequences against the non specific target and you found a degree of matching between your probe and the non specific target?

You can find the specific sequence of IFN-alpha from NCBI database . Take the gene sequence make your primers pair using Primer Quest ( IDT).

In fact I only hope to retrieve real data (through an API, for example) to use data interpolation techniques.

I believe that there must be some API that allows me to retrieve data from sensors as close as possible to real time.

Mattia is correct. For a home, the CR-39 is for a minimum of 90 days and the RAD7 is for 1 hour house measurements over a few days. To find hot spots, use the RAD 7 (be aware of Rn-220 response)

thanks a lot Frederic Lepretre for answering my question, actually I work on placenta tissue and whole blood and I checked the expression in NCBI but I didn't check on GTEX.

High-yield savings accounts.

Short-term certificates of deposit.

Short-term government bond funds.

Series I bonds.

Short-term corporate bond funds.

S&P 500 index funds.

Dividend stock funds.

Value stock funds.

Solving an equation in real time is about the speed with which it can be done. Also, the context would be important. Typically heat flows are relatively slow -- seconds rather than milli- or micro-seconds or less (as might be appropriate for various wave systems, esp. electromagnetic ones). Weather problems include heat/diffusion effects & equations, and need to be solved over hours to provide "real time" predictions.

That being said, if the problem is say essentially two-dimensional (plates, perhaps), then the solvers can be quite fast. Large 3-D problems with complex geometries would be harder, but could probably be done with some clever computational methods, such as

* precomputing the mass/stiffness matrices (perhaps using a lumped mass approximation),

* solving the equations coming from an (implicit) ODE solver for the finite element/difference/volume equations using iterative methods and good starting approximations (say, a low order predictor to feed into the implicit solver).

Hello Thenmozhi Thangaraj

Different journals will have different requirements for what you call proof. It is difficult to make one statement to cover all the journals so I suggest you may need to contact a journal that you are interested in and ask them what they need exactly if this is not clearly stated already on their website in "Instructions for Authors".

Frank Lloyd

No special clinical need or product measurement.

Thought it could have been a blank control in some metabolic intervention experiments.

There are no data on insulin levels in indigenous mice population within their normal food environment, rather than WT one produced in the lab.

Reason: similar comparison can be projected between two human populations.

try to use 1 HKG and see how it works

if it gives you fantastic result

you are good to go

Thank you for all your answers, I will try it. :)

One of the important areas of research for Industry 4.0 is human performance enhancement. You may find these articles relevant to your question and some of the references in the papers may also be helpful.

Check these helpful links.

https://randomnerdtutorials.com/esp32-firebase-realtime-database/#:~:text=Go%20to%20Firebase%20and%20sign,needed%20and%20click%20Create%20project.

@ Paul Rutland

Thanks .

Yes all the samples should amplify the product .

I will check it and will share the results.

if your curves are on the left side, it may be primer dimers; on the right side, it may be genomic contamination. I think you can increase Tm in the cycles or decrease the primers concentration. and it would be better. BTW the way 284 is high but you can get the result.

Thank you very much

For isopropanol precipitation use EQUAL volume. Follow the Trizol protocol. For 1 ml Trizol, add 200 ul chloroform to extract, mix well, spin at high speed and remove aqueous top phase, which should be 500-600 ul. Dont take any interface, no need to get every last drop. Add an equal volume of isopropanol and vortex. No need for 4oC overnight. Leave at room temp and spin at high speed , then wash pellet in 70% ethanol. Usually no need for DNAse if your primers for RT-PCR are in two separate exons.

Maybe these articles will help you.

1) Neuro-Inspired Eye Tracking With Eye Movement Dynamics (thecvf.com)

2) Best practices in eye tracking research - ScienceDirect

Universal control channel https://t.me/universalcontrol

What about these?

* https://randomnerdtutorials.com/esp32-firebase-realtime-database/

* https://randomnerdtutorials.com/esp32-influxdb/

BTW.: g..gl. is a great resource to answer such questions on your own.

It may be that your dna is absorbing onto the plastic of the tube or it is being degraded by nucleases or acid pH.

You can store lyophilised and make up in TE just before the assay or add some non amplifying dna or rna to your standards before storage. The cold dna will block the absorption sites on the plastic tubes. Siliconisation of the storage tubes may also help

Vipul Batra thank you, I will check it.

Respected James R Knaub Sir,

Thank you so much. Further, I will ask about some problems with it.

hi,

Non-Specific binding (NSB) refers to an occurrence of an antibody binding to unintended proteins, receptors, or transporters. This binding of assay antibodies is not correlated with the specificity of the antibodies.

ips for Reducing Non-specific Staining in IHC

For more information kindly refer this link:

Best wishes..

refer this link:

hi,

kindly refer this link:

Best wishes..

hi,

A multilevel inverter is a power electronic device that is used for high voltage and high power application because of its characteristics of generating a sinusoidal voltage based on several DC voltage levels.

A switched capacitor (SC) is an electronic circuit element implementing a filter. It works by moving charges into and out of capacitors when switches are opened and closed. Usually, non-overlapping signals are used to control the switches, so that not all switches are closed simultaneously.

for more info:

Yes. The dye is made for qrtPCR. According to Promega, the dye has very similar spectral properties to those of SYBR Green (https://www.promega.com/~/media/files/products%20and%20services/islides/is022-gotaq_real-time_pcr.ashx). Any instrument capable of extiting and measuring Fluorescein and/or SYBR Green should work with BRYT Green as well. To my knowledge, this applies to all available qrtPCR instruments on the market.

Teng Zhang Take a look:

1. https://www.scitepress.org/papers/2018/69057/69057.pdf

2.

3. https://www.diva-portal.org/smash/get/diva2:459937/FULLTEXT02

Thank you for your reply. It's still not clear to me how this applies to quantitative variables, like for instance gene expression measurements. A corresponding variable in bahavioral science might be something like "time spend at some place" or "distance moved (upon some stimulus)" etc. How can ony analyze such data using contingency tables?

For miRNAs from cells or tissues, the cDNA is routinely diluted 1:5 to 1:10 depending on the initial RNA concentration to ensure a final cDNA amount around 10-100 ng per reaction

Thank you @Zulaykha Khurshid for the replying

But the problem I am facing is that I can amplify my gene of interest with semi quantitative pcr with 20 ng/ul but if I use this concentration in real time the gene of interest shows undetermined

Dear Abdelmadjid,

can you clarify what kind of data you need?

It is important because there are many repository of real-time medical data however it is not known that you need this kind of data.

Track the jammer, and use information from that track to reduce the effects of the jammer on other tracks.

Seven necessary properties of highly secure, network-connected devices: a hardware-based root of trust, a small trusted computing base, defense in depth, compartmentalization, certificate-based authentication, security renewal, and failure reporting. https://www.microsoft.com/en-us/research/wp-content/uploads/2017/03/SevenPropertiesofHighlySecureDevices.pdf

Lavanya Malini, Yeah primers is not a problem here. I will try with new batch of chemicals.

Thank You.

Nivedhitha

Start with a simple question. First problem: reliable data about sediment, collected on a consistent basis, is hard to find. Begin by writing a simple questionnaire based on what you already know about sediment. Ask yourself a simple question such as: 'What was the proportion of biological input to this rock sample, ranging fro very high for a limestone to very low for an evaporite, with shale somewhere in the middle.' Then collect 1000 rock samples at random from your target area. Now, list your personal 'quick look' percentage estimate of its biological content for each numbered sample . Next ask 100 colleagues and students each to make the same quick look estimate of biological input for each of 10 samples, with their numbers chosen at random by each anonymous participant; note and list each percentage estimate, until you have 1000 numbered estimates. Finally, write a simple program to analyse your 2000 data points using MS Excel.

Use Google Earth Engine platform

I think for sentence clustering it is better to use Sentence-BERT model a modified version of BERT based on siamese network, which very suitable for sentence clustering. Please refer to the original paper of sentence- bert

yes you can

Dear Awad

of course! Matlab/Simulink environment has different functions for real-time applications. I did this for PLC Siemens S7 and Arduino as I/O platforms. If you have any questions, please do not hesitate to contact me with this e-mail: sa_rafatnia@sut.ac.ir

Yeah, it is also a good option, Sir Bruno Martin

Ultrasonic flowmeter:

Hello Abdul Rouf

RT-PCR, also known as Reverse Transcriptase PCR, typically measures RNA expression levels. In RT-PCR, complementary DNA (cDNA) is made by reverse transcribing of the RNA template with the enzyme reverse transcriptase.

This technique is used to qualitatively study gene expression and can be combined with real time PCR (qPCR) to quantify RNA levels. RT-PCR is used in research laboratories to study gene expression, for example in experiments to distinguish exons from introns, and can be used clinically to diagnose genetic diseases and monitor drug therapy.

Also, RT-PCR is commonly used in the diagnosis and quantification of RNA virus infections (e.g., human immunodeficiency virus, hepatitis C virus, corona virus) and the analysis of mRNA transcripts such as those produced by translocations commonly associated with non-Hodgkin's lymphomas, leukemias, and sarcomas.

Gene expression profiling is likely to have a major impact on molecular diagnostics and will depend on RNA analysis using RT-PCR and possibly high-density arrays.

So, it is not always extracting DNA directly from the cells and then preparing the samples for qPCR. Sometimes, we have to go for reverse transcription step to prepare cDNA and then do qPCR.

Hope this information helps!

Best Wishes.

Thank you

probably it will increase delay

I agree with @Imra. Nevertheless I throw in a couple of examples. See the attached. Best wishes David Booth see the following response

Transiently transfected cells are usually used for Real-Time PCR 24-96 hours after transfection.

It depends on your sample frequency, which is limited for performance of DAQ. another issue should be concerned is the number analog of a processing control system. the quality of data will be bad or real-time data might be good if you collect as much data in process as possible.

Dear Rosita Husain,

Greetings!

You can check the following links

1) https://marketstack.com.

2) https://www.nyse.com/market-data/real-time

Karishma Patil

Take a look at these papers:

1. https://arxiv.org/pdf/1907.03670.pdf

2.

3.

4. https://openaccess.thecvf.com/content_cvpr_2018/papers/Yang_PIXOR_Real-Time_3D_CVPR_2018_paper.pdf

5. https://www.scitepress.org/Papers/2020/93304/93304.pdf

Hello Joel,

You can do a melt curve analysis and make sure the primers are specifically amplifying your gene of interest as well as has a single peak. Additionally, you can run an agarose gel for the products and confirm the amplification.

Respected Sir,

Refer this link:

Hope this information is useful.

Dear Miel Verkerken

Snort. Snort is a very important tool since it is the de-facto standard for intrusion detection systems. This Linux application is simple to set up and can be configured to monitor your network traffic for intrusion attempts, log them, and perform a predefined action if one is discovered.

The most of intrusion prevention systems employ one of three detection techniques: signature-based, statistical anomaly-based, or stateful protocol analysis.

Kind Regards

Dear Youngil Sohn,

As you have said so well, this is not a simple problem, for that it will be necessary to choose a tool or a combination of tools which is at the same time powerful and provided with smoothness and this to reach an adjustment including these two criteria. antagonists.

We can offer basic AI algorithms such as Traveling Salesman Problem (TSP), Deep Neural Networks or techniques that are part of Machine Learning.

For more deatils and information about this subject i suggest you to see links on topic.

https://realpars.com/power-plant/

https://www.bing.com/newtabredir?

Best regards

Dear Amit Kumar ,

The minimum sampling rate fs is limited by the Nyquist sampling rate. The sampling Nyquist rate is greater equal 2 fmax where fmax is the maximum frequency contained in the original signal. If the an waveform is sampled with fs=> Nyquist sampling rate you can restore the original waveform by an interpolation process.

So, you have to tell us what is thye maximum frequency contained in your original signal.

If your sampling time is 5 x10_7 s and then you resample it at 100x10^-7 s then you decreased the sampling frequency by 20 times. This will scale down the frequency of the waveform by 2o times. Sure it will lead to great distortion of the signal if it is under sampled.

I do not know how did you decide on such new sampling frequency.

Under sampling leads to aliasing distortion of the signal.

Best wishes

Hi, I think you should use the decide function to select N-way by condition and write expressions like NQ(Registration.Queue)<=NQ(Registration2.Queue), the NQ() means the number of people in the queue, and the (Registration.Queue) means the queue of the registration service desk. Hope this will be helpful.

There are currently many simulators in electronics that are very good. But each simulator is to some extent aimed at a specific type of electronic device. There are also more universal simulators, but in most cases they are for amateur purposes. I think that in order to get good advice from colleagues here, you need to specify for what type of electronic devices you will use it.

Irene-Love Amoah Your samples have curves looking like obtained with significant inhibition of PCR. You can try two-three different protocols/kits for DNA extraction.

veins is ok. you can ns3 simulator aswell

I recommended CTAB with mycobacterium it works good.

Certainly not, the traditional style is boring and is not creative. We need a style that is liberated from the routine steps of thinking to reach the conclusion. I don't have the alternative, but someone else may have.

Conventional PCR (C-PCR) has been used to detect specific target genes in various microorganisms (5, 6, 13). Nested PCR (N-PCR) was developed to improve sensitivity but can give erroneous positive results due to DNA contamination @ Buhari Suraka

Lee Jun Yang ,

What you are describing appear to be two different problems, one compounding the other

For the first problem

1) Which ICA implementation are you using?

For the second problem

1) For the real time capture what sampling rate are you doing the capture?

2) what are you using to capture the signal in real time(e.g. are you using ALSA on Linux or are you using Windows)?

Note that the RM Cortex-M7 of the Teensy 4.0 is a slow processor compared to an Intel or AMD

Thanks a lot for gracious participation respected Dr ranjeet

Phuong Bui Attached document is about AI , Wifi and IoT. may be relevant for indoor positionning.

I need codes to encrypt and decode images in Matlab for the following algorithms.

AES, with key 128 bit

AES, with key 256 bit

RSA, with key 1024 bit

RSA, with key 2048 bit