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Radioprotection - Science topic

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while doing my experiments on VERO cells, testing a radioprotective agent 24 h after irradiation (6 Gy), i run an MTT assay and i found viability above 100% (around 138%) and i couldn't explain it.
any idea
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MTT is a less sensitive method compared to other available assays for analyzing cell viability although cell viability can be measured but do not expect the answer about the proliferation of cells directly from MTT. It is cell number which is determining factor in MTT. The healthy and rapidly growing cells exhibit high rates of MTT reduction to formazan while the dead or inactive cells fail to do so.
If you are using MTT assay, then you could observe the difference in viability not due to the change in amount of cells, but also due to the change in mitochondria activity. So you need to think very carefully what your assay measures and then think if you need some additional controls or a second assay. Best will be trying some other sensitive assays.
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Less toxicity is one of them
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Please refer the attached file as an additional information
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We have found in our previous work that the tooth loses its mechanical strength and composition after radiation treatment. hence now we are checking for a protective layer (which is either organic or inorganic) to apply before radiation treatment to promote preventive effects of radiation among head and neck cancer patients. kindly suggest some materials which has proven protective effects against radiation?
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Dear Rudolf
The method you suggested will have problems. If good care is taken during planning and still the tooth is the radiation field because it cannot be avoided; if it is avoided, then you will miss the tumour too. In such a casee, if you shield the tooth, very likely it will shield the tumour giving it less than optimal dose and lead to higher chance of recurrence. Another problem is that whatever material you use, there will be secondary radiation at the interphase between the tooth and the shield which may increase the damage. Moreover dental problems are more likely to be due to salivary irradiation problems; salivary glands are very sensitive to radiation.
This the reason why such materials or methods to shield teeth have not been designed. Of course some one sometime may design such a thing with a new idea.
All the best
Narayanan
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As many antioxidants are being shown to be effective against radiation exposure in controlled conditions like animal experiments or with the cell lines, but how can we use them in practical situations for radiation leaks or accidents?
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Antioxidants can play a role in preventing indirect cell kill by neutralizing free radicals. However, they cannot prevent direct cell kill due to single-hit double strand DNA breaks..