RACE - Science topic

Future of all on earth in year 2050 to 2100...

All negative trends for example

Pollution contamination thought pollution broken societies toxins in food

MATERIALISTIC BLIND RACE...?

"DNA is SO unpredictable that they are either fractals or something less predictable, thus a gene is never known to manifest into a trait, debunking hereditarianism and vindicating CRT" (Ohnemus 2024).

Yes, so they should follow DEI.

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You are invited to participate in a survey, entitled “Race-based Traumatic Stress and Microaggression in Nursing Faculty of Color.” The study is being conducted by Queyka SaintLouis and Shannon Avery-Desmarais, Department of Adult Nursing of The University of Massachusetts Dartmouth, 285 Old Westport Rd, Dartmouth, MA 02368, 508-910-6598, qsaintlouis@umassd.edu and savery@umassd.edu. The purpose of this study is to examine the link between the academic environment and the professional experience of the nursing faculty of color. Your participation in the survey will contribute to a better understanding of the importance of nursing faculty of color in diversifying the nursing workforce and the effects of race and ethnicity in the lives of nursing faculty of color. We estimate that it will take about 16 minutes of your time to complete the questionnaire. You are free to contact the investigator at the above address and phone number to discuss the survey. Risks to participants are considered minimal. However, if you do feel discomfort, you are encouraged to reach out to your primary care provider. You can call 988 in the event of a mental health emergency. You are also offered to contact the racial equity support line at 503-575-3764. This resource is available Monday through Friday 10 AM to 7 AM Pacific Standard Time, however, you may leave a voicemail should you call outside of regular business hours. https://www.linesforlife.org/get-help-now/services-and-crisis-lines/racial-equity-support-line/ . There will be no costs for participating, nor will you benefit from participating. You will receive a $15 Amazon gift card to thank you for your time. Identification numbers associated with email addresses will be kept during the data collection phase for tracking purposes only. A limited number of research team members will have access to the data during data collection. This information will be stripped from the final dataset. Your participation in this survey is voluntary. You may decline to answer any question and you have the right to withdraw from participation at any time without penalty. If you wish to withdraw from the study or have any questions, contact the investigator listed above. If you have any questions or would like us to email another person for your institution or update your email address, please call Queyka SaintLouis and Shannon Avery-Desmarais at 508-910-6598 or send an email to qsaintlouis@umassd.edu and savery@umassd.edu .You may also request a hard copy of the survey from the contact information above. To complete the survey, click on the link below: https://umassdartmouth.co1.qualtrics.com/jfe/form/SV_1TxTsmJNTlXJcOO The password for the survey is M@ther123 If you do not want to receive any more reminders, you may email us at qsaintlouis@umassd.edu and savery@umassd.edu or follow this link to opt out of future emails [HTTP://LINK TO REMOVAL URL]. This study has been reviewed and approved by The University of Massachusetts Dartmouth Institutional Review Board. If you have questions about your rights as a study participant, or are dissatisfied at any time with any aspect of this study, you may contact - anonymously, if you wish - the Institutional Review Board by phone at (508) 910-9880 or email at akarberg@umassd.edu. IRB Approval Number: 24.026. If you agree to participate, please press the arrow button at the bottom right of the screen otherwise use the X at the upper right corner to close this window and disconnect. Thank you.

Throughout history, homosexuality has been cause for rejection and discrimination, and those who had this sexual condition were labeled and stigmatized socially, prejuzgados, imprisoned and treated as criminals, being victims of assault, torture and murder. For a long time this condition has been considered even an unnatural aberration and mental illness in different psychiatric diagnostic manuals. Currently in Spain, despite the existence of laws allowing homosexuals to marry and adopt children to form a family, as well as the recognition of their civil rights, remain a social group that suffers from social exclusion and suffer the scourge of homophobia, rejection and violence of those who regard them as different or sick. Perhaps that is the reason why either time to integrate to homosexuality as a plot more than the specialized social services, to combat the situation of social exclusion, and develop the resources and means to combat it.

¿Por qué la homosexualidad no es un colectivo que se incluya dentro del ámbito de los Servicios Sociales Especializados?

A lo largo de la historia la homosexualidad ha sido motivo de rechazo y discriminación, y aquellos que tenían esta condición sexual eran etiquetados y estigmatizados socialmente, prejuzgados, encarcelados y tratados como a delincuentes, siendo víctimas de agresiones, tortura y asesinato. Durante mucho tiempo esta condición ha sido considerada incluso como una aberración antinatural y una enfermedad mental en diversos manuales de diagnóstico psiquiátrico. Actualmente en España, pese a la existencia de leyes que permiten a los homosexuales a poder contraer matrimonio y adoptar hijos para formar una familia, así como al reconocimiento de sus derechos civiles, siguen siendo un colectivo social que sufre exclusión social y padece el azote de la homofobia, el rechazo y la violencia de aquellos que los consideran como diferentes o enfermos. Quizás por ello ya sea hora de integrar a la homosexualidad como una parcela más de los Servicios Sociales Especializados, para poder combatir la situación de exclusión social que sufren, y desarrollar los recursos y medios necesarios para combatirlo. 

I am interested in relationships between photovoice or auto-photography as research methods and social-spatial difference, either as captured in the photographs, or as embodied or lived by the participants. I would particularly appreciate suggestions of literature from the past 10 years.

Recommendations of reading on participant-photography and social-spatial difference would also be relevant in this case.

recently i have been trying to amplify a p450 gene but it kept failing. I was able to amplify a small fragment of the gene but never the whole gene so i decided to go for RACE experiment. With RACE i was able to amplify the gene and then i sent it for sequencing. I have obtained the sequence but both 3' and 5' frangments do not align with the computed sequence i have. Could it be that the sequence was originally not correct? What would be the suggestion? Should i just go ahead and decide new primers? anyone experiencing the same thing before?

Thank you very much

In my anthropological researches I am concerned with names.

I remarked here in the Research Gate, as in the worldly society, that many members have got first names as (Ansar) which make a confusion with the last name, the family name (Ansari).

Are the 2 names family names?

Are there any differencies between the 2 of them?

I'm wondering if anyone can help me? I'm new to this subject and I'm finding it easy to find research regarding white researchers conducting research in 'ethnic minority' communities, but I'm struggling to find research regarding a black researcher conducting research in predominantly white communities.

I'm wanting to know anyone can point me in the right direction regarding research specifically detailing the impact of race with regards to a black researcher conducting research amongst predominantly white participants in a working-class area (that is predominantly white)?

I'm interested in research that has been conducted in the UK though I'm also open to research conducted in other countries.

I am working on a study of African American crime and detective writing in the 1950s and 1960s. What are the best books or articles on the race riots in Chicago, Newark and Lod Angeles in the 1960? Thank you

Hi, I have six transcript variants for my gene of interest for which I am doing RACE (both 5'RACE and 3'RACE). I have a problem in understanding that when I clone, for ex. 10 clones from each 5'-RACE and 3'-RACE and then I do sequence alignment that will give me the idea for different transcript variants expressed in cell but how would I combine the 5'-RACE and 3'-RACE sequences to get full length sequence for each transcript variants that expressed in cell?

Hi everyone,

I'm trying to amplify an unknown sequence of a patient with a deletion in the last exon of the target gene (ECT2: https://genome.ucsc.edu/cgi-bin/hgTracks?db=hg38&lastVirtModeType=default&lastVirtModeExtraState=&virtModeType=default&virtMode=0&nonVirtPosition=&position=chr3%3A172750682%2D172821474&hgsid=730703519_g4wtnxAdAV1gXohOVmwU0EiXzEbi) using the 3' RACE technique. I've managed to obtain the wt sequences of this region in the two positive controls, but once I cloned and sequenced the PCR product obtained from the patient's DNA, what I found is that I had basically amplified and cloned a totally different gene on another chromosome. This is probably due to the fact that the first RACE round amplified non-specific regions, even using a very specific forward primer, since I've had no choice but using a high number of cycles to be able to see sufficiently intense bands. Thanks to everyone who will take some time to read my question and help.

For good reasons America developed a segregated system of higher education, which currently means a number of colleges remain open to black people only. In the US, and other countries, students are also segregated on grounds like religion and gender. Is such a system compatible with the aim of a full and rounded education?

I have looked at "white racism" research which generally shows up in contexts where there is a majority/minority dynamic. I am looking for racism research particularly directed towards Pakistani community, sometimes including Indians and Bengalis as well. In particular, I want to look at "othering" research focusing on Pakistanis/ browns, and othering by Middle Easterns, Black communities and South East Asians in particular.

What is genocide? A quick search gives the following definition: the deliberate killing of a large group of people, especially those of a particular ethnic group or nation.

Now, in this scenario, there's no actual killing. However, it has long been understood that race is not a biologically valid trait. We are all one species and there hasn't been time for actual biologically valid separations from groups to evolve. So, what differentiates one group of people from another is culture.

If a group of people are required to give up their cultural heritage, then that ethnicity is gone. Obviously this is a fairly charged question, but I think the RG community is up to answering it honestly and rationally.

I am looking for articles or any other resources on issues related to social class mobility and how issues of exclusion due to group membership- race, gender, caste, religion etc might impact this mobility. 

Thank you!! 

I want to define both 5' and 3' ends of a lncRNA using GeneRacer Kit (Invitrogen). I don't know if my transcript has a poli(A) tail and the manual recommend using an oligo(dT) primer to reverse transcribe the mRNA if I want the sequence information for the 3' end...I wonder if the GeneRacer RNA oligo that we ligate to the deccaped mRNA is enough to the oligo(dT) primer to anneal and synthesise my cDNA (in case it is not polyadenilated). Can anyone help me?

Hello everyboy, i need help How to do Rapid amplification of cDNA ends (RACE)?

i have got the sequence of two new genes in my species (Insect) now i want to do Rapid amplification of cDNA ends (RACE). so i need complete protocol and guidelines .

your valuable guidelines will be appreciated.

Thanks.

M Hafeez

I am performing RACE PCR and in experiment of 5' and 3' PCR with the UPM and gene specific primer.... I m getting numerous band. Normally we should not get any band in the gel. I do have isolated RNA and then checked the RNA integrity... it showed me perfect. But I don't understand why I am getting numerous bands... U suggestions will help me to troubleshoot.

Thanks in advance. 

Hi,

I am interested in academics' experiences of attending conferences. How have you found them? Have you experienced or witnessed gender, academic status, 'race', class or otherwise bias?

Is this the status-quo? Is it particular subjects where this is experienced? What can be done about this?

I am interested in knowing why many older people are abused or deceived economically by ignorance or vulnerability.

Many vulnerable elderly people are deceived by their own families in order to obtain an economic benefit.

In addition they can also suffer physical violence by relatives or carers for the simple fact of being defenseless before the physical or verbal aggressions of the same ones. 

My research team is surveying literature on issues of Eugenics in Asia. This might relate to practices and policies of forced sterilization/abortion for women at risk of bearing children with "birth defects," especially intellectual disability/Down syndrome, birth control measures and the like that control population.  It also is rooted in nationalism and racism.  All interest and information is welcome for the discussion.

Hello

Do I need to have a 1-5 ug of total RNA to get good result from RACE by Invitrogen method?

when political violence, electoral violence and criminal violence came together what results appear in governance and development? I need evidence and rich texts. would you help me?

I am working on a non-model plant. The sequence of my organism does not exist. I have a RT PCR product that i amplified with primers of the plants within same family. The length of my amplicons are between 70 and 150 bp. We have one unopened RACE kit. But i am not sure to use it. 

Do you think that British Political plays are more successful than the American ones? and if so, why this happens? is it due to the audience, the censorship in America or the high quality of  British plays?

The age of consent is the age at which a person is considered to be legally competent to consent to sexual acts.

Do you agree that the age of consent is more than 18? Is it a valid index?

Are all of the people mature in this age?

I am working on Financial Inclusion in India.  I would like to discuss on this topic with researchers, academicians, policy makers and practitioner to enrich my study.      

As we know black colour is dominant over white as per genetic inheritance. Acordingly the question is aimed to know the possible irish colours  of the children out of the marriage of the parents of blue and brown irish colours.

The respondents are a minority and marginalized community

They have suffered injustices and discrimination

They were driven out of their land and are homeless

They cannot fully participate in governance and political participation

Their language is classified by UNESCO as critically endangered

My assumption is this is because they remained silent

I would like to be guided on questions that would elicit why they opt to remain silent rather than speak up.

How this relation has been transformed from the apathied to post Aparthied

I wonder if anyone knows of an instrument that measures prejudice or stereotypes directed against religious people in general (preferable in a Scandinavian context). If not, do you know any instrument that can be used to measure prejudice or stereotypes directed against various religious minorities.

Education and racism, inequality, unfairness, manipulation, control, bias, gender gap, discrimination.

I am planning to do my next research on “Uniforms and its psychological impact”. I would like to know there any standard procedure or instruments to measure the impact of uniforms on public. Interested researchers can contact me so that we can do a better cross-culture study on this topic.

I want to know about the society's view on rape, and how the society influences the act of raping.

Hi,

I am looking for recent polls (for any country; preferably for European and/or OECD countries) that asked questions about how people judge different forms of discrimination. I am less interested in questions about whether or not people think that particular forms of discrimination are more or less widespread or whether a particular form "is a problem" (this I found online for many countries and for many recent years).

I am more interested in polls/data that show to what extent people think that different forms of discrimination violate norms prevalent in contemporary societies such as norms of fairness or meritocratic principles. Do people think that all forms of discrimination are equally wrong or unjust? I'd like to know the numbers.

For Europe, the most recent survey I found dates back to 2003. Please see the linked document (pdf) and the section "Opposition to discrimination" therein. 

Thanks and best wishes,

Sebastian

Is there any anthropological research addressing the question: Why may many of the female workers be aversive to their  female co-workers but may have a strong preference for male co-workers in the workplace in the North America or elsewhere? Can anyone direct me relevant references or research materials?

In Australia 'Leave early or stay and defend' is used, and 'Ready, Set, Go!' is used in the US. 

I wondered if one exists for Europe, in particular France?

Is someone currently carrying out research on the franchise in catering market? Italy, Spain, German?

I want to know the political hierarchy or social organization of this particular tribe.

I would appreciate hearing more about your project especially lesbians if at all possible.

Thank you,

Rebecca White,

University of Illinois at Chicago and University of Rwanda

People with disabilities have sexual needs that can not be met normally. In addition, families maintain their sexuality as a taboo subject. I would like to investigate people with disabilities who access prostitution, how are the relationships, what specific needs they have ... I do not find much information about it. Thanks.

I have read previously that in discourse analysis we should not place participants in a box by presenting their demographic characteristics, because the approach seeks to emancipate individuals from such categorisation (highlights inequalities and oppression that some groups face), so we don't want to begin by placing them in a box. Any comments or references please??

There are different organizations and institutions that are involved in dealing with orphans, and abandoned children; Kafala, adoption, foster-care, orphanages, modern boarding schools, group homes, and other practices, what are the major challenges or the best practices among the above?

I have some objects and I must make a group of some objects with known characteristics for each cluster before the analysis.. 

In class I study about cluster analysis but the characteristic of each cluster is known after it.

For example there are 20 students, and I want to make a group of some 'best' students , with characteristics : high GPA, tall, etc... 

Thanks before..

Recent research has revealed that the practice of the female genital mutilation in countries like Ivory, Somali and Sudan is mostly the influenced by patriarchal dominance in these nations in Africa (Poggioli, French Activists Fight FGM,2009; Monagan,2010). For this reason, my project intend to investigate the roles men can play in the prevention of  Female Genital Mutilation using  their supremacy in the societies in Africa.

I thought that this was a common critique of race research, but I cannot seem to find any sources about this. Am I wrong? Am I just using bad search terms? Please help! 

I'm currently investigating the impact of ecotourism on Bali's cultural landscapes, i.e. traditional rice terraces and water temples.

I've already come across Cole's (2012) analysis of water inequity in Bali. 

Thanks in advance!

Autonomy is a polisemic concept, it doesn´t say much by itself until it gets into a cultural and political contexts. I´m elaborating a state of art about territorial indigenous autonomy, emphasising the rol of traditional knowledge and how it is shaped by the interactions of indigenous and local organizations with enviromental institutions in charge of accomplish the purposes of the Convention on Biological Diversity. I also would like to know which are the discussions around indigenous autonomy in the global environmental governance arena.

If you want to share some opinion, documents or articles about these topics I'd appreciate it.

Please add your answers in english, french or spanish.

Thanks

We are seeking a measure that:

- does not have substantial overlap with depressive symptoms;

- has been validated with diverse populations with respect to race, ethnicity, immigration status, housing status, etc.

I am considering the cultural differences between the countries of the Levant and Iraq.  I am searching for a useful measurement tool.  I considered Hofstede's dimensions, but they seem too coarse for the smaller differences in the region. Looking for something a bit more sensitive.

I want clarification so that I know if I have two strata or one strata. I know that I already have race as one of my strata but was wondering if states are considered strata or not. 

Sampling Design

Research Question: I would like to understand to what extent the racial group (non-white or white) of Americans influences their degree of nationalism. I define nationalism as found in a dictionary: “the strong belief that the interests of a particular nation-state are of primary importance.” I will operationalize nationalism as wanting to “build a wall” between the Mexico and U.S border and rate it on a scale of “strongly in favor, slightly in favor or not in favor”. I believe that people who are white will want to restrict immigration flow from Mexico to the U.S. On the other hand, non-white people are more likely to be less nationalistic and will welcome diverse populations and thus will not want to build a wall. My dependent variable is nationalism and my independent variable is race.

Sample Design:

Theoretical population: My theoretical population would be citizens from every state in the United States that ascribe to different political views.

Sampling frame: My sampling frame would ideally be an equal amount of non-white and white citizens from every state in the United States that ascribe to different political views.  My sampling frame will include two individuals from every state: one non-white and one white. These individuals will be selected from a computer device that generates numbers. The machines will be programmed with area codes from each of the 50 states and will randomly generate the last 8 numbers of a phone number (either cell phone or landline). If there is no answer, the machine will select another citizen of the state. The individuals will be asked if they are white or non- white when taking the survey, so as to make sure one of each is chosen. The individuals will also be asked if they are 18 and older.

My sample design would be disproportional stratified random sampling. I would stratify the sample equally by racial groups: 50 % nonwhite and 50 % white racial groups. I would do so to ensure that my population is not representative of the United States because the United States population consists of 77% of whites and most whites tend to favor Republican views which would result in skewed data towards attitudes that are in favor of limiting immigration since that has been the historical trend. In short, I do so to control for bias of political party preferences due to people heavily identifying with ideologies/policies of their group.

I would then stratify it by state, because there are almost an equal amount of republican “red” states as well as democratic “blue” states. Since states are already divided into categories, I would simply select one person as white and another person as non-white.

Hello, 

I'm trying to identify the unknown gene (phosphoglycerate mutase) from a nematode by RACE technique (Clonetech, Takara)(The CDS orthrologue gene in C.elegans is 1.5 Kb ). I performed 5' and 3' RACE reaction together with 25 cycles (annealing temp. 65C; kit recommended 68C)and then observed on 1.2% agarose electrophoresis. 3' RACE product gave a 600 bp size but 5' is not presence. Then, I performed the 5' RACE reaction with 5 cycles (total 3c cyles). The result showed approximate 10kb of 5' RACE product size (In figure). So, i want to know "Is possible that the nematode gene has 5'utr up to 10kb?" or it's the error on electrophoresis.

Thank you so much,

Sarit

During my experiment (cut stem inoculation technique) to identify the isolate aggressiveness of Macrophomina phaseolina and out of 15, 3 isolates found very aggressive.

Can we go for race identification. If yes, then what procedure i should follow.

Thank you.

Dear all,

I would like to ask is there any other ways to check the integrity and quality of the cDNA synthesis by using the RACE kits? other ways than the one suggested by the kits/protocol.

Palaeoanthropology and palaeobiology in human evolutionary biology have well-substantiated divisions of the genus Homo and are relatively clear about the line within the genus leading down to modern humans. Are there any similar taxonomic divisions in present human populations? So far, I have found that the answer to this question is no. I would like to give a detailed account of why this is or is not the case.

What are the current positions in the literature on the matter from a systematics/taxonomic point of view?

Thank you in advance for your time,

Phila. 

Dear all,

I would like to ask for general recommendations to anyone with experience of using the aforementioned kit for their RACE PCR.

I am currently having difficulties on using the kit for both 5' and 3' RACE of which I am running out of options on where to troubleshoot, and hence any alternative point of view which I may have missed would be most welcomed.

At the moment, I have a total RNA sample that I know contains my mRNA of interest, as using the same sample, I managed to synthesize first strand cDNAs. To my understanding, since I used oligo dT primers to do so, the synthesized sequence should be originating from only mRNAs present within. With use of designed degenerate primers on the cDNAs, I managed to obtain a partial fragment of my gene of interest some 700bp long of which I used to design gene specific primers for RACE.

Unfortunately, after following the protocols outlined from first strand synthesis to nested RACE PCR, I have so far been unable to obtain any bands of any kind. All this is in view of using the same RNA aliquot obtained earlier, with integrity and concentration prior to use verified via denaturing gel electrophoresis via nanodrop. The primers I designed (sense for 3' RACE and antisense for 5' RACE) have been deemed to be good and later confirmed via amplification of the correct size of band.

From this result, and assuming the other components have been administered as sufficient, my main hunch as to where it could go wrong is on the first strand synthesis for both strands, of which the 5' CDS and 3' CDS primers (at 0.5µM per reaction,functions as oligo dT primer shall function) may have difficulty in binding to the polyA tails due to the initial total RNA concentration being low, but again I iterate that this is all a hunch and nothing is certain.

Should anyone else out there be familiar with RACE PCR of a different kind, attached with this email is the User Manual for the aforementioned kit, which may prove useful for comparison. Any help is much appreciated and I extend my thanks in advance for all the kind souls out there willing to do so :)

hello guys, I am planning to do RACE but confused to choose the kit. If anyone having good experience, please suggest me the name.

At present I am optimizing the right condition for use of RACE kit with Genomic DNA ? Does any one have any experience , as the kit referred to application of total RNA.

In other words, does it append two-four cytosines to the 3'-end of newly synthesized cDNA strands like MMLV and SuperScript II do?

I've been trying to amplify 3' splice variants of a hormone receptor gene, and I initially had great success. However, now I cannot repeat my results. The first 3-4 PCR's ran great, but now I only get a smear. I have replaced my primers because I was worried they were contaminated, but I still get the same smear. When I use different primers as a positive control, I get good clean amplification, so I think my cDNA is still OK. I need this specific reaction to work so I can amplify enough DNA to sequence the splice variants - any thoughts?

The answer to this question is key to better understand the cancer risk associated with some germline polymorphisms and its relation to race and ethnicity.

10x Taq buffer and MgCl2 were provided separately, and I am using these at 1x concentration.

Positive control and water controls are working good, but the target cDNA has not yet been amplified.

I want to amplify the uncharacterized gene. First step, I will aplify and sequencing full cDNA of gene.

What is the best (quality and price) the RACE kit ?

(First choice, invitrogen  and   SMARTER RACE, takara  or etc.)

thank you

I know the sequence of my gene but want to do the RACE to confirm the position of 5 end prime and 3'  end prime. Now, I  am confused about how RACE works in this case and we can say by seeing at results(RACE) that this is the start or end point of a gene?

Please help me to understand the RACE technique more precisely and its applications.

Thanks

Thanks in advance.

I need to know how to design GSPs for RACE. Iam going to use Clontech Smarter RACE kit for the first time. My RNA quality is good. Even the GSPs I designed for semi-quantitative works well. Whether I should use the same GSPs or design other ones with higher Tm. My question is how to proceed with cloning my gene using RACE amplification, starting with the primer design for the same.

Iam sending the link of the related paper. http://www.sciencedirect.com/science/article/pii/S0161589009007664

I have 200 bp known fragment. for RACE PCR. I am using Clontech kit. I tried all the possible primers with UPM (with kit), but never got the desired band. I synthesized new RACE cDNA but still the problem persists. The degenerate primers work well to get partial fragment, but GSPs dont. I can't use one degenerate primer with UPM because of high Tm difference. Could you suggest the solution for my problem?