Science topic

Quercus - Science topic

Quercus are a plant genus of the family FAGACEAE that is a source of TANNINS. Do not confuse with Holly (ILEX).
Questions related to Quercus
  • asked a question related to Quercus
Question
6 answers
We need to find molar mass of flour from quercus.
Relevant answer
Answer
You can use the tools available on the internet such as Molar Mass, Molecular Weight and Elemental Composition Calculator
For the additional information please kindly consult:
Zarroug Y et al. (2020), Extraction and Characterization of Tunisian Quercus ilex Starch and Its Effect on Fermented Dairy Product Quality. Int J Anal Chem. 2020 Aug 4;2020, doi: 10.1155/2020/8868673. PMID: 32831842; PMCID: PMC7424496.
  • asked a question related to Quercus
Question
2 answers
I am looking for reliable data processing to convert cubic meters of Quercus cerris (Hungarian oak) wood into CO2 equivalent. Thank you.
Relevant answer
Answer
Hi Fabrizio,
We have to start with a boundary condition. The boundary condition is that all the carbon in the Quercus wood is converted in CO2 (like burning wood in a stove till only inorganic ash remains.
If this boundary condition is met, then the problem is reduced to a good estimate of the carbon content of (Quercus) wood.
Let's assume that we take 1 m³ as a standard volume to convert. The carbon content of trees is usually calculated from wood volume equations (in m³) which include basic wood density (Mega-gram of dry biomass per m³) and a conversion factor for the %C in dry biomass (Houghton et al. 1990).
Basic wood density is defined as the oven dry weight of wood divided by the volume of wood. It is commonly considered as an indicator of wood quality and varies widely among species. On the other hand, a concentration ratio of 50% is used to convert tree biomass to carbon stock for operational level purposes. This simplification has been proposed as a default value by the IPCC (Houghton et al. 1990), even though carbon content varies between tree species. See also:
Variation in carbon concentration and basic density along stems of sessile oak (Quercus petraea (Matt.) Liebl.) and Pyrenean oak (Quercus pyrenaica Willd.) in the Cantabrian Range (NW Spain).
Available from the URL:
Example:
For Quercus petrea the %C varies between 46 and 50 or in MgC/ha between 184 and 216.
For Quercus pyrenaica the %C varies between 45.6 and 50 or in MgC/ha between 94 and 121.
To go from C to CO2 (equivalent), apply the rule of three, based on the molecular weights of C and C02.
Hope this helps,
CHeerz
Frank
  • asked a question related to Quercus
Question
2 answers
In case if we don't get the volume equation, Biomass expansion factor and root shoot ratio for a particular species, how to calculate the carbon stock of a tree?
Relevant answer
Answer
Thank you sir
  • asked a question related to Quercus
Question
4 answers
For my master's thesis I am conducting research about the feasibility of establishing carbon forests in the middle east and generating revenue through the selling of carbon credits, therefore, I need formulas of forest trees volume as a function of age/time to insert in NPV calculations and the Faustmann model, however, I did not find anything, particularly for : Aleppo pine Pinus halepensis, phoenician juniper, and Valonia Oak Quercus Aegilops.
  • asked a question related to Quercus
Question
2 answers
Ink for writing was derived from galls harvested from oak trees - see http://www.medievalcodes.ca
for more info.
Can readers guide me to any research on how the tonnage of galls required for ink making was supplied and where the main areas of production were?
Relevant answer
Answer
This is outside of my area but I do know that acorns were one of the major food sources for hogs fattening in the Fall. Today people just think of acorns as a possible twisted ankle. This had led to a tragic decline in young oaks in US cities. Most complaints are that they are messy. Gettting back to medieval trees, I would look for areas of pasture for pigs. Unlike chestnuts and wallnuts, people can't live on acorns fof long, only as a famine food. Acorns are just too hard to turn into meal on a regular basis and we certainly can't chew them. The Early Medieval Irish Chronicles record mast falls in the second half of the seventh century and no one has ever figured out why.
  • asked a question related to Quercus
Question
2 answers
WANTED
We are looking for a frozen or in ethanol-preserved Cerambyx paludivagus for barcoding
SCIENTIFIC REWARD
Relevant answer
Answer
Dear Derradj,
Thank you very much for your collaboration offer.
Since the last records of C paludivagus that I know date from several decades ago, I had even thought that this species was extinct.
From your address I suposse that the material will be from the mountains near Constantine.
Best
Luismi
  • asked a question related to Quercus
Question
1 answer
my study area located in the forest region having Shortleaf Pine and Oaktree, thus in the Canopy Method I am trying to get the Crop Coefficient value of these trees but did not get anything.
Relevant answer
Answer
Interested
  • asked a question related to Quercus
Question
3 answers
I am not a botanist or familiar with the botanical Code ( https://www.iapt-taxon.org/nomen/pages/intro/dates.html), but would like to establish which is the correct spelling of this species of oak found in western Cuba. The OD clearly spells it sagraeana ( https://archive.org/details/cihm_40072/page/n61/mode/2up), but modern Cuban authorities appear to spell it sagrana, while a brief internet search seems to throw up virtually equal numbers of both spellings (wikipedia, for example, spells it sagraeana). Any reason in the botanical Code why the original spelling has been abandoned in favour of sagrana. Thank you!
Relevant answer
Answer
Thank you Allen James Coombes for your insight into the botanical Code.
  • asked a question related to Quercus
Question
3 answers
I recorded several porcupines throughout the Chihuahuan desert in 2019- specifically in the Querecho Plains. There are few large trees, mainly Western soapberry, ( Sapindus saponaria), found around waterholes, and invasive Salt Cedar, ( Tamarix ramosissima). However, the deciduous Shinnery oak, (Quercus harvardii) is the dominant plant in the landscape, and is said to be the densest forest in the world! Shinnery oak gets its name because it is no taller than your shin.
Photo: Two noisy porcupines mating in a Western soapberry
  • asked a question related to Quercus
Question
4 answers
I detected some data about the presence of holm oak (Quercus ilex L.) in Andorra but I don't know if the information is reliable. Also anyone know examples of holm oaks in mountains? At what altitude?
Thanks in advance!
Relevant answer
Answer
Please take a look at this useful PDF attachment.
  • asked a question related to Quercus
Question
3 answers
My objective of my work is to develop prediction models for tree age
based on diameter at breast height (dbh). Study species: Abies borisii-regis, Fagus sylvatica, Pinus nigra, Quercus trojana, Quercus cerris.
I have looked at these papers:
(Dobrovolný, L. & Tesař, Vladimír. (2010). Growth and characteristics of old beech (Fagus sylvatica L.) trees individually dispersed in spruce monocultures. Journal of Forest Science
Loewenstein, Edward & Johnson, Paul & Garrett, Harold. (2000). Age and diameter structure of a managed uneven-aged oak forest. Canadian Journal of Forest Research-revue Canadienne De Recherche Forestiere - Canadian Journal of Forest Research 30(7):1060-1070
Łukaszkiewicz, Jan & Kosmala, Marek. (2008). Determining the Age of Streetside Trees with Diameter at Breast Height-based Multifactorial Model. Arboriculture and Urban Forestry.
Rohner, Brigitte & Bugmann, Harald & Bigler, Christof. (2013). Estimating the age–diameter relationship of oak species in Switzerland using nonlinear mixed-effects models. European Journal of Forest Research. 10.1007/s10342-013-0710-5. )
Cοuld anyone help me to find an accurate way to estimate tree age [Abies borisii-regis, Pinus nigra, Quercus trojana] using DBH?
Thank you.
Relevant answer
Answer
Hope this demonstration will help.
Thank you
  • asked a question related to Quercus
Question
1 answer
The thermal disspation method after Granier requires a species-specific calibration to quantify sap flux density correctly. I am looking for a species-specific calibration for Quercus suber (cork-oak).
Relevant answer
Answer
Hi Simon,
To my knowledge Jan Cermak from the University of Brno (Czech Republic) is one of the best scientists internationally concerning the measurement of sap flow in trees. Jan has performed these measurements on a lot of species. You should contact him to know whether he and/or a Spanish colleague have measured sap flow in Quercus suber. Jan is a forester working on whole tree water relations and macro structure.
See:
Cheers,
Frank
  • asked a question related to Quercus
Question
5 answers
We are aiming to study the elevational distribution of plant species. Currently we are focusing on tree Pinus and Quercus.
Relevant answer
Answer
Generally speaking, almost all the native species are suitable.
However, from the practical point of view, it is necessary to take into account also the previous use by human population and if the distribution of a species is effectively correspondent to its potential distribution.
As an example, we studied the elevation shift of Picea abies and Larix Decidua in the Italian Alps.
In our case it is fundamental to know where the upper potential limit is reached thus we can say that the plants that will grow at higher elevation are exploring unprecedented height. On the contrary, if the potential limit was influenced by cuttings or pasture or any other reason this means that at present times the treeline is much lower than it should be and part of the occurring regrowth is due to the reconquering of an already suitable space.
This situation is quite frequent in the European Alps.
I hope it help you to clarify some issues,
all the best,
Marco
  • asked a question related to Quercus
Question
6 answers
We launched a study on germination of cork oak (Quercus suber). Our tests failed because of contamination, despite the sterilization of the acorns by the bleach solution. How to eliminate these contaminations that block germination? What is the best way to germinate acorns? and if you have any documents on this subject. Thank you in advance for all your help.
Benmahioul
Relevant answer
Answer
Freshly seeds can germinate easily. After a long time storage > 2 months, embryonic dormancy will settle and the seeds will be difficult to germinate.
  • asked a question related to Quercus
Question
3 answers
Does anyone have evidences of Quercus suber as invasive, exotic or even naturally stablished anywhere? Base in a bibliographic search aparently is only present out of its area as plantation!
Relevant answer
You can find info here:
It seems naturalised in the Baleares and Canary islands
  • asked a question related to Quercus
Question
2 answers
I'm looking for studies concerning extractives' quantification in Oak wood. Is there articles or studies going on about this subject ?
Relevant answer
Answer
I didn't know this article, thank you it is very interesting and helpful !
  • asked a question related to Quercus
Question
6 answers
We are working on the invasive capacity of Quercus suber in the Canary Islands, where it is an introduced plant. In our work, the capacity of regrowth from its superficial roots is the apparently most common form of propagation of this tree. We do not know if this is a mechanism of habitual propagation of the species in its natural zone.
Relevant answer
Answer
Thank you very much for your reply. The current forest comes from some plantations about 200 years ago, but it is quite dense, and as you can see in the image the importance of root sprouts is very large. I imagine that the structure of the vegetation that we find in the Canary Islands is more similar to the dense forest than to the open formations. Thanks again for your comment.
  • asked a question related to Quercus
Question
5 answers
I'm currently working on how plants can find carbon sources for their metabolism, except from photosynthesis.
I found cases of mycoheterotrophic nutrition in non-chlorophylic plants and orchids, where plants build a parasitic relashionship with mycorrhizal fungi. Could other plants, like the cultivated ones, or trees, use mycorrizal nets for carbon nutrition from time to time, even when they could realize photosynthesis ? In other words, is non-clorophylic plants way of surviving a new function they developp, or is it a "natural" way of doing for every plant that they push to the extreme ?
Another case is the one of Quercus ilex, that would establish a temporary mycoheterotrophic nutrition, in spring when the root stocks are depleted and the leaves are not grown yet. Is there any study about it ?
I've already red the work of Garbaye J. 2013. La symbiose mycorhizienne. Une association entre les plantes et les champignons. éd. Quae. pp 70-88, 102-105. and work of Marc-André Sélosse.
Thanks for your answers.
Relevant answer
Answer
Yes they extensively do as an integral part of symbiosis ....
  • asked a question related to Quercus
Question
2 answers
I am planning a greenhouse experiment with Q. robur and U. laevis to understand the rhizosphere processes of these trees ex situ. Could anybody point me towards a reliable supplier of tree seedlings? (Preferably in Germany)
Thank you!
Relevant answer
Answer
Hallo Anastasia,
ich antworte mal in Deutsch, da Du ja auch in Deutschland auf der Suche nach Samen bist. Der wahrscheinlich einfachste Weg, an gutes Samenmaterial für heimische Bäume zu kommen, ist irgendeinen staatlichen Revierförster zu fragen, also den zuständigen Förster für eine bestimmte Region. Wer das in welcher Region ist, erfährst Du beim zuständigen Amt für Ernährung, Landwirtschaft und Forsten (Name des Amtes kann von Bundesland zu Bundesland ein bißchen variieren.
Gruß
Martin
  • asked a question related to Quercus
Question
11 answers
I have selected a first generation of individuals from a few parental specimens of Quercus pubescens; they show red crowns in autumn as the parents do. This color seems to be unknown in Q. pubescens. I am looking for any information from anywhere about other individuals and/or groups of Q. pubescens with the same trait. Thank you.
Relevant answer
Answer
Thank you for sharing your photos!! It is very nice color. I hope you will be able to propagate this plant in one way or another. I am convinced it would be a success for home gardens and municipal green areas.
  • asked a question related to Quercus
Question
3 answers
Hello, 
I am looking for references on trees wood density for most of the Mediterranean species e.g. Quercus infectoria, Pinus brutia, Ceratonia siliqua... Any suggestions?
Thanks in advance,
Ralph
  • asked a question related to Quercus
Question
4 answers
I am looking for information on the altitudinal limit for Quercus cerris and other deciduous oaks that grow in the Anatolian region. There seems to be some inconsistency and I would like to know if I have missed some research relevant to the region. 
  • asked a question related to Quercus
Question
10 answers
Sampled in Greece, Athens, Tatoi
07.05.2016
plant: Quercus coccifera
caterpillar: Lymantria dispar
primary parasitoid: Cotesia melanoscela
Thanks a lot in advance
Vladimir
Relevant answer
Answer
With photos is difficult to ID at species and genus level, however  try to see if your specimen follow this characteristics of Gelis.
Characteristics of Gelis include: 1. Body size (mm) (excluding antennae and ovipositor):<10mm; 2. Position of spiracle on Tergite 1 (T1 of metasoma):clearly behind the centre; 3. Shape of aerolet in forewing:other; 4. Colour of face:only black; 5. Metasoma compressed:dorsal-ventrally; 6. Size of Ocelli:small; 7. Length of antennae:shorter than body; 8. Length of ovipositor:not longer than body; 9. Wings:present; 10. Colour of wings:mostly clear but with dark patches/spots; 11. Sternaulus (on mesopleuron):short; less than 0.5x length mesopleuron; 11. Sternaulus (on mesopleuron):long; more than 0.5x length mesopleuron; 12. Shape of face in lateral view:flat or only weakly bulging; 13. Sternite on T1(viewed laterally):sternite not extending past spiracle; 14. Shape of T1(viewed laterally):evenly curved; 15. Number of teeth in mandibles:1 or 2; 16. Patterns on metasoma:same colour throughout; 17. Length of T1 vs T2:subequal in length; 18. Sculpture on mesoscutum:finely pitted, many hairs; 19. Width of T1 (viewed dorsally):gradually widening from anterior to posterior; 20. Glymma on T1:absent; 21. Sculpture on metasoma:smooth with a semi-glossy or satin appearance at least on T2; 22. Propodeum length:Propodeum very short (not reaching beyond coxal insertion).
  • asked a question related to Quercus
Question
4 answers
I want to perform DNA extraction from Quercus leaves (several diferent species) and the DNeasy kit yields very low DNA concentrations with not very good quality.
Relevant answer
Answer
Thank you very much for your help Aseesh. We will keep in contact
  • asked a question related to Quercus
Question
4 answers
The inventory for the CBM-CFS3 (carbon balance model from the canadian forest sector) requires volume-age curves for each stand and I cannot figure out how to obtain these curves.
I can find information about dbh, aboveground biomass and volume but I cannot find how this relates to age. Can anyone tell me what I am missing here? I am specifically looking at mexican oaks (Quercus castanea, Q. crassifolia, Q. laeta, Q. obtusata y Q. rugosa). 
The model developers, Kurz et al. (2009) state: "Forest management agencies and industry have built up large libraries of yield tables to describe the accumulation of volume in the merchantable portion of tree stems as a function of stand age. To enable the use of these data sources, CBM-CFS2 was modified from using biomass over age to CBM-CFS3, that uses merchantable volume over age data to simulate growth."
Where can I find these tables with volume-age tables?
Relevant answer
Answer
Here it the large file document
  • asked a question related to Quercus
Question
7 answers
Dear scientists
I am going to extract the oil from the fruit of genre Quercus, I want to know is that the drying of the fruit before the extraction can affect the quality of the oil to be extracted and which is the best mode of drying which you can propose
Best regards
Relevant answer
Answer
Oil quality not affected if you used low temperature. You can try de-moisturized by drying seeds in an oven under vacuum at 40-50 Celsius degree  for 24-36 h according to moisture content of seeds before extraction of oil.
  • asked a question related to Quercus
Question
6 answers
We are performing a study that intends to relate the natural regeneration of Quercus species - Q. pyrenaica, Q. suber, Q. rotundifolia, Q. robur, Q. faginea - in the center-eastern part of Portugal (Castelo Branco region), with the environmental factors in order to use that data (oak trees occurrence and other woody plants) as soil and climate indicators for different purposes (e.g., suitability for crops and forest stands).
The soil and climate attribute suitability and constraints per species to analyze are: soil pH, soil texture, soil type, lithology, temperature, rainfall, humidity, bioclimatic indices.
Thanks in advance
Relevant answer
Answer
Hi, sorry for cross posting. I would be grateful if we work on the origin and dispersal of Quercus species. If anyone would have interest and as our peers already have mentioned here.
  • asked a question related to Quercus
Question
4 answers
The larva was found on Quercus, and I took an image of it.
Relevant answer
Answer
Thanks a lot Dear Rudolf! Any idea what species it could be?
Gy
  • asked a question related to Quercus
Question
16 answers
I am looking for some kind of standarisation for determining ring curvature radius for archaeological deciduous Quercus (cf cerris). I have used the the "trigonometry in an isosceles triangle" after Paradis (2007) and Paradis et al. (2011) to calculate the radius of curvature but this results in literally thousands of unique measurements. I was wondering if there is a standard for grouping radius curvature dimensions together (into bins) for statistical comparison. For example: fragments with a radius of 0-50 mm are considered strongly curved and those with a radius between 50.1-100 mm are moderately curved etc. Given that Quercus cerris grows to a diameter of up to 2 m what is considered weakly curved? This may well be available in the French literature but as I cannot translate from French to English any assistance would be appreciated. 
Relevant answer
Answer
Nathan,
I am not too familiar with coppicing and pollarding. What I see is farming, where fast growth species such as popular (Populus sp.) or maple (Acer sp.) are grown to ~25 cm and then harvested for firewood. With staggered planting, the supply is constant. I suppose with coppicing and pollarding you would not necessarily have to worry about distinguishing between young growth trees and limbs, since young trees would be uncommon?
  • asked a question related to Quercus
Question
4 answers
hello, everyone. 
As I said, I have already finished my sequence amplification experiment of 4 markers of more than 200 samples. Now I have to calculate the species identification ability of each marker, which is the resolution of each markers as well. I am planing  to do it with BLAST. As I understand, I need to build a database first, then use each sequence as a query to test the E-value of the blast ranked sequences and statistic analysis the resolution in the end.
I don't know if this is the right way to calculate, or is there any more detailed and correct instruction to tell me how to do with it. 
By the way, the species is Quercus spp, a kind of plant. 
Any suggestions will be appreciated! Thank you very much !
Relevant answer
Answer
Hello Xiong,
There are two broad options for testing species identification using barcoding: 1) Percent Identity (PI) and 2) Genetic Distance (GD).
For PI most researchers use BLAST, however this method has been shown to either have similar or worst ability at correct identification and typically has far worst incorrect identification rate. Thus, using this method typically leads to a much higher probability of false identification then the GD methods. See Parmentier et al. (2011) for an example in rain forest trees (attached below). This method is also not robust to changes in the length of the sequences and is especially error prone with closely related species.
Thus, I would suggest using a GD method. These methods typically require building a global alignment and then gather distance data using a model of evolution. Then using these distances you can either build a phylogeny and look at what nodes sample fall in or just simply examining the matrix of distances and see what the closest match is. The Parmentier et al. paper has a good explanation of these methods and I also attached two other papers that are highly cited in the barcoding world that are great reads and may be helpful.
Best Regards.
  • asked a question related to Quercus
Question
3 answers
Hi all,
I am looking for an allometric relationship between Leaf Area Index (LAI) and Woody Area Index (WAI) of Quercus species. Have you ever seen such a study focused on any species of Quercus (Oak)?
Regards,
O,
Relevant answer
Answer
 I have not come across such metric, although I did study oaks. From first principle I would assume such metric is not a species but rather but a site characteristic. Particularly, site management history will impact on both LAI and WAI, but with a time different time lag 
  • asked a question related to Quercus
Question
15 answers
These look-alike proliferation tumors slough off on the most magnified photo in the group (1st photo in the group, right side). The tree host species is red oak Quercus rubra.
Relevant answer
Answer
Symptoms on oak infected by Pezicula cinnamomea.
  • asked a question related to Quercus
Question
3 answers
I am planning a research project on the interactions between 'hedgehog galls' and bur oak (Quercus macrocarpa) in southeastern Québec (Canada).
Relevant answer
Answer
Try contact Dr. Spungis in Riga or Dr. Mamaev in Moscow - these are leading taxonomists for gall midges and may suggest something useful 
  • asked a question related to Quercus
Question
13 answers
As we know, coppice species like Quercus sp create several stems per tree making them somewhat hard to estimate their hydrological processes accurately. Does anybody have any ideas as to how to do it?
Take a look at attached file to see a coppice tree.
Relevant answer
Answer
Hi, dear Omid Fathizadeh.
What about the leaf xylem water potential?
  • asked a question related to Quercus
Question
4 answers
I am currently studying the phyto-ecological characteristics and geographical distribution of Quercus ithaburensis in Lebanon.I would like to see if such studies have been realized in the Near East region, where this species thrives, and have related articles. Thank you.
Relevant answer
Answer
Dear Jean,
You can find most of the publications related to Q.ithaburensis in the reference list at the end of my paper mentioned above by Wendy.
If you need a PDF let me know.
The distribution of Qii and the characteristics of its populations in Lebanon are undocumented so your work can fill the gap and bring valuable information about this special oak.
Good luck
  • asked a question related to Quercus
Question
4 answers
To understand the "state" of the biodiversity of Quercus in a Mediterranean region, some questions must be answered such as; how does the vegetation recover, how does it regenerate after fire, and how does it turn back to the initial conditions?
Relevant answer
Answer
Hi,
Which Quercus? Different Oak ecosystems exist in the Mediterranean (Ilex/Coccifera/Suber for instance), with very specific ecological profiles, different fire regimes and therefore different response type to fire. So before telling you any preferred method, if one exists, what kind of Oak ecosystem do you want to study ?
  • asked a question related to Quercus
Question
3 answers
Dear colleagues,
Currently I'm working on provenance trials and progeny tests of oaks in Germany and I need more information about two pedunculate oak (Quercus robur) provenances from the Netherlands. Their names are: Nuen-02 and NL-3. They were used in a provenance trial established in 1996 in Germany and were characterized as "special provenances" (Sonderherkünfte) i.e. showing especially good phenotypic traits (growth, stem form etc.). In spite of an exhaustive search I did, I couldn't find more information. Can you provide me more info about this provenance or some tips how to search for it?
Thanks in advance :)
Relevant answer
Answer
Hello -Regarding oak provenences surry but I am not a genetist I am a selviculturalist for some information on oak provenence you can ask to Fulvio Ducci a collegue of mine.  happy new year
  • asked a question related to Quercus
Question
3 answers
Recent papers or works. Thanks
Relevant answer
Answer
Oak and hornbeam trees also grow in the forests of northern Iran.Hornbeam leaves decomposed faster than oak and help to regeneration them.Pure oak forests with poor soils can be seen.
Hornbeam trees cause less light reaches the trunk of the oak trees.Oak trees will result in better quality.
  • asked a question related to Quercus
Question
2 answers
Hi all :)
I´ve been trying to amplify DNA from different sources - young plant leaves from Quercus, Pinus, etc.; as well as mycorrhizae tips. And i am getting many bands (1 to 4) which is not what i hoped for - in most of the cases, and if i increase the annealing temp, then i get no bands at all.
I´m looking for a good genetic marker for species identification. I will be assessing this with the use of other primer sets too, like ITS2, RBCL and MATK, etc. The thing is that TRNH-PSBA is described as very informative for this type of studies, but since i´m kind of new to plant genetics, i´m afraid i might be missing some basics :)
Did someone struggle with this as well ?
Shall i sequence the heavier fragment (~500-600 bp) by extracting it from the gel, since this should be the main sequence i need?
Another thing that confuses me ... I´ve come across several protocols with a broad range of temperatures and times, and have not been successful using the "plant DNA barcode protocol" temperature of 64ºC. I get bands in the range of 58~60ºC (annealing) but not above.
Thanks in advance !
Relevant answer
Answer
Hi!
PCR conditions vary in minute details between labs, so annealing tempartures from the literature will always be guesses (or points to start optimizing from) to any user elsewhere, at best. Also, try to get high quality primers synthesized & purified.
The spacer you are using is useful for more detailed studies within genera and species, especially if you plan to sequence. Simply because it is a spacer and not a slowly evolving gene!
But then again, chloroplasts are tricky. In every "new" organism, there may be new mutations that make primer sites useless, or often there are repeats that mess up things. There should be Fagaceae complete chloroplast genomes available in GenBank, you could try and look at the exact region you want to amply in those sequences, for any repeats or unusual stuff.
Finally, the hint for my database on the web which lists hundreds of chloroplast primers:
(go to database - table - and filter for genes of interest). You may be able to find alternative primer sequence suggestions close to the region you are interested in.
Hope this helps somehow!