Purchasing - Science topic

Hello everyone,

I would like to ask have anyone works on the HS-5 cell line? May I know how long it takes for the cells to confluence after revive? My HS-5 cell line is newly purchased. After revived, the cells not confluence after 2 weeks. Kindly advised on what should I do. Thank you.

Hello,

As a part of my research work, I need to detect the wild type p53 level in MCF-7 cells using western blotting. For this purpose i would like to know which antibody should i purchase (i.e) either monoclonal antibody or polyclonal antibody. If it is mnoclonal antibody, will it have any effect if it is produced by immunizing the animal with recombinant human p53 protein expressed in E.coli? Further, if there is any cat log no: available for the product (p53 antibody), i kindly request you to provide. Please do the needful.

Thanks in advance.

I want to create multi-layer biomedical scaffolds and i need graphene or graphite sheets.

I am just wondering which theory best describes moderating role of price conciseness between purchase intentions and behavior?

Arif

I am working in a paper as I need a dataset for customer purchasing products from a market. where can I find it ?

Dear fellow researchers,

I have been looking to buy some updated database for Raman spectroscopy of different organic, inorganic minerals, polymers etc., (updated with new entries at least in 2020). Could any of you suggest some sellers or links for the same.

Thank you all in advance.

Best regards

i came across the various kits avalable for AKP activity in mammalian system. But i want to check AKP activity in group of LAB. WIll the same kit works for the same or i need to purchase other one? Also can we check the activity manually? Is there any protocol avalable?

I'm looking to purchase premium plagiarism detection software. Which one is the most reliable and accurate on the market?

Usually for plagiarism checking Turnitin software is used, that is not freely available. Is there any plagiarism checker software that can freely available or can be used without purchase?

This is my first introduction to statistical analysis. I am wanting to understand how to test the theory that influencer marketing will drive sales. Two yes/no questions were asked and answered including "Do you follow any influencers?" and "Have you ever purchased anything seen used by an influencer?" From the outset the data seems to show that those who do follow influencers are inspired to purchase. But it also appears to identify that those who do not follow are also influenced. I'm really trying to work out the best formulas for analysis. If anyone could help, it would be greatly appreciated.

I'm hoping to identify microbleeds in the brain tissue of a rat, so thought to stain for iron. I have perfuse-fixed frozen tissue on slides in 10um sections and I bought Prussian blue but would love some advice or an existing protocol - how best to make up the solution, prepare the tissue and times/temperatures to stain it!

(This is the specific product I purchased: https://www.sigmaaldrich.com/catalog/product/sigma/03899?lang=en&region=GB)

Recently, my UG students analyzed a protein for drug binding site on DogSiteScorer. Can we published this data by citing the website or we have have to purchase the license?

Public procurement denotes the attainment of goods, works, or services required by governments through contracts. Public procurement is usually plagued with covert practices and exclusive forms of corruption in Bangladesh. The cost of development finance is much higher in public procurement than that of others in Bangladesh. There have been no unified rules or laws regarding government purchases since 2006. The Government of Bangladesh enacted its ever first act of public procurement, the Public Procurement Act, 2006 (PPA, 2006), and the Public Procurement Rules, 2008 (PPR, 2008). The procurement entity must disseminate procurement information through electronic medium i.e. Electronic Government Procurement (hereinafter e-GP). Despite passing over an era since its enactment, the role of the PPA, 2006 remains seriously under-researched area, specifically there is no research on the issue of the grievance redress mechanisms. The study has been done using a qualitative case study methodology, backed by both primary and secondary documentary analysis. Based on empirical data, this paper explained the role of digitalization in the process of government procurement and depicted changes that have been brought by the implementation of e-GP in government purchases. However, it concluded that the grievance redress mechanism in the process of procurement is only existing in the policy papers which need mandatory implementation for a transparent and accountable governance system. Despite the significant changes and up-gradation in digital government procurement, it is hardly found that the GRM process is digitalized. And the mass tenderers are yet to be acquainted with the improvised system apart from the consistent efforts of the government.

Hi.

We are working on tree growth/health enhancements and were wondering what sort of bacteria or fungi (or a consortium thereof) biostimulants or biofertilizers (plant growth/health promoting bacteria or fungi) are available for purchase in Europe (i.e. comply with EU standards)? Can anyone help?

I am looking to purchase a Laser micromachining system for fabricating microfluidics devices with 35micron rounded channels from PDMS, PMMA or glass. What is the best make and model to achieve this type of feature resolution and what system parameters are the most important ones that I should be aware of? Thank you

I am just wondering which theory best describes moderating role of price conciseness between purchase intentions and behavior?

Arif

I am just wondering which theory best describes moderating role of price conciseness between purchase intentions and behavior?

regards

Arif

I am having some issues with Bovine serum I have purchased and am wondering if there is a better serum to purchase that others have had better success using? any advice would be appreciated.

I am conducting a customer satisfaction survey on visiting and purchasing customers of a shopping mall, therefore no database to understand the population. As I am conducting the research targeting only visiting customers, from day to day numbers are different. So can this be an infinite population? can I use G power to calculate sample size? or any other method to identify the population? Thank you so much

Hello,

I have purchased MIN6 and INS-1E cell lines (Beta cell) from AddexBio and I'm culturing them in flask based on the protocol they provided. However, for some of my tests, I have to culture them in 6 and 12 well plates. But 3 days after plating, they start to round-up and lose the normal protrusions that they have while attaching to the surface. I know that the round-up cells are not dead.

this pattern starts from a corner of well and goes forwards towards the center of well. I have changed my well-plate plastic material, culture media, incubator, etc. and this problem is still there.

I should mention they look normal in flask but once I plate them in well-plates, I observe this issue.

So I wanted to ask if you have any explanation or suggestion to help me with this issue? I would be thankful for your comments.

I have attached here an image showing how they look like.

Thank you.

Hello, I need to recruit respondents for my PhD research survey. Is there any reputable place to purchase or rent an email list?

Currently I'm working on a project related surface ship simulation. We need to simulate a plenty of ship types. I modeled propeller, rudder etc. But hydrodynamic forces are modeled as hyrodynamic derivatives (hyrodynamic coefficients, maneuvering coefficients ). For some ships, we generate our hyrodynamics data, but as I said, we need lot more. We dont want to spend time on CFD. We need direct data of Hydrodynamic of some ships. Is there such a company/institute/university/organization etc.? Any suggestion?

Hello

I need poly SIBS for my PhD project because of its suitability for medical metal coating. However I can't find commercially available poly-SIBS. I contacted with K.neka corporation in Japan but they didn't want to send the poly SIBS sample for medical use. Even though it's not for medical usage, only for academic purposes I couldn't find it anywhere. The only product I could find is poly SEBS from Fisher which doesn't include exactly the same chemistry as SIBS.

Hello everyone,

I would like to know how other battery groups store their In foil. It's the first time I purchased it and the Sigma package came totally not airtight like for Li or Na and just in regular packaging. Is that normal? Thanks.

I am really confused with the variables that we will use in our study. We aim to identify the direct influence of consumer attitude toward pop-up ads on their purchase intention. In our framwork, the purchase intention is the dependent variable. However, the consumer attitude itself can be influenced by pop-up ads, so I believe that it cannot be labeled as the 'independent variable', since it depends on the pop-up ads.

My question is, what type of variable is the attitude, as well as the pop-up ads, if our main focus in the study is the attitude and purchase intention?

Hello,

I am currently writing my bacherlor's thesis and I am now at my results part. However, I am not quite sure which test I should use now to answer my hypothesis, two-way ANOVA or multiple lineair regression?

My hypothesis are

H1: Purchase intention is higher for advertisements that use human influencers (vs. virtual).

H2: Self-esteem will moderate the effect of the type of influencer on purchase intention.

In which self-esteem and type of influencer (human vs virtual) are the independent variables and purchase intention is the dependent variable.

Thank you.

Hi everyone

I want to study the role women consumers play in the purchase decision-making process. Marketing literature states 5 roles( initiator, information search...) Since these are on a nominal scale, I am finding it difficult to build my model.

Can anyone suggest a scale which can be used to measure the same?

I wanted to make triethanolamine oleate as a lubricant.

So I purchased oleic acid. There were two different oleic acid (industrial grade) in the maket: liquid and semi-solid.

What is their difference?

I used liquid as trusted and good quality. I mixed them together without any catalyst or pressure-temperature conditions and the result was unsatisfactory.

What is suitable condition for this esterification?

Thanks

I am looking for patterned ITO substrates for ready purchase to make my solar cells. Can anyone let me know the details of any Indian agent?

I want to know where you got cell lines if you recently purchased MH7A cells. Thanks

Dear all,

Greetings.

I am working on my PhD where I am exploring the role of women in purchase decision-making w.r.t Consumer electronics. It is an interdisciplinary study where I have studied and combined decision-making aspects from the literature on women's empowerment and role in purchase decision-making as established in marketing.

My framework includes testing the impact of 3 demographic factors (Age, Education and work status- IDV, nominal scale) on Consumer Self-Confidence, which is having 5 constructs (DV- Likert scale).

The outcome- what role women have in decision-making.

It is believed, that more the Confidence, the more should be the role in decision-making.

I am seeking guidance from experts to understand what software and tools will be best to study the above state? IDV are nominal, DV are Likert scale and outcome is again nominal.

Any suggestions for betterment are welcome :-)

For my research I'm trying to show how/whether or not trust mediates the relationship between anthropomorphised online agents and purchase intention. I've used existing scales to measure these variables but I'm not sure how this is done in SPSS - do I need to create a new single variable for anthropomorphism, trust and purchase intent combining the questions from the scales, or do I need to do something else?

In the attached photos you can see that when I put eigenvalue higher than 1 (which is of course supposed to be) I get 6 components, however my conceptual model proposed 7. For illustration I put in eigenvalue higher than 0.976 to ask the following question: is it possible to get this number to 1? Right now with 6 components, attitude and purchase intention are on the same component however in my conceptual model attitude leads to a higher purchase intention. Is this solvable?

Kind regards,

what methodology is recommended to show the relationship between the consumers purchasing behavior with their demographic nature??

Hello. I am trying to perform cell dissociation for the whole adult mouse brains (pnd90). I used Worthington Papain Dissociation System for a while, but after dissociation, cell viability was too low and the kit is a little too expensive for me to purchase it constantly. Does anyone have a cheaper protocol using media such as Hibernate, and enzymes like Papain with Dnase etc.? How much papain and Dnase would be enough to dissociate cells of a whole adult mouse brain and how long should papain incubation be in this case, according to your experiences? Thanks in advance.

A link for purchase would be helpful!

Online purchasing has become the fastest and easy mode for household product purchases around the globe. It reaches your home according to your specified time frame.

Do you think that the products we buy are value added as when we purchase through a showroom we get the advantage of a real time demonstration of the product with various advantages and disadvantages comparison with the other products and the representatives of the companies pulling us to buy their products providing us instant GIFT coupons and offers to attract them.

What I am trying to explain is the advantages and disadvantages of buying a household product from a showroom Vs online.

Do you think that this opportunity of seeing and buying from the showroom is better than reviewing the products online and buying the product.

Lets have a serious discussion on this topic.

Thanks,

Nujum Niyaz

Lincoln University

Our lab purchased the Spectramax ID3 and have encountered an error in the built in computer due to an issue connecting to the hard drive. Has anyone had experience with this and know of a resolution or the cost of repair?

We are measuring "Purchase intention" and have therefore made a framework where Four variables, Involvement, Argument Quality, Source credibility and Information Usefulness together make up Purchase intentions.

We have conducted a survey with a number of questions for each variable mentioned above with an answering scale of 1-6. The aim of the study is to compare the mean of each variable inorder to see if the two groups (Genz & Millenials) the survey was sent out to differentiate in some way. We are also wondering how to "combine" these four variables inorder to measure the mean for Purchase intention for each group.

Thanks in advance

We need to purchase a paraffin embedder and cold block. Please send me reviews on your experience with specific model numbers. New vs refurbished? Companies/models to focus/stay away from. Thank you in advance!

I am planning to work with interstitial fluid (ISF). Is there any place that I can buy normal ISF? or, Can you please suggest the composition to make in the lab? or, Is there any convenient method to extract and use it?

Does anyone know a source where I can purchase it premade or if you have a spare one laying around and willing to donate?It is a microdot stamp to create microisland cultures of astrocytes and autaptic neurons.

Please help

Sam

#astrocytes

#neurons

#synapse

It's my humble request to Research Scholars.

I want to collect data and analyze it using AMTI force plates. I do not have Visual3D purchased. Any leads would help.

Dear all,

Can anyone suggest me a good plasmid with Tetracycline resistant gene to purchase ? (resistant only to tetracycline)

We have a quantitative research study title of "The Effects of the COVID-19 Pandemic on the Online Purchasing Behavior of Senior High School Students".

In this study, our goal is to determine the effects as well as certain factors that make up for the changes in online purchasing behavior due to the COVID-19 pandemic on senior high school students.

What I would like to ask is what would our quantitative research design would be: descriptive, correlational, ex post facto, quasi-experimental, or experimental?

I would appreciate your thoughts and suggestions, thank you.

Both the ELISA and another testing method will work.

I sent a purchase request to IGM Bioscience (Palo Alto, USA), but did not get in touch. Thanks.

I am currently working on a money supply simulation and there are some pieces of the puzzle that are still missing. One of them has to do with the purchase program of the ECB.

Say I am a pension fund which is holding a government bond which the ECB wants to purchase for 1000 EUR. With the purchase program the ECB creates money to purchase this bond but that money is digital central bank money which can not be transferred to my account. My question is, where does the money that I eventually receive om my account come from? How would that look when explained with the balance sheets of me (the pension fund), my bank and the ECB?

Thanks in advance,

Stef Kuypers

Hello everyone,

I am currently want to confirm the production of Cre recombinase by Western blot in my cells after infected with the Lenti-Cre virus since we did not see the recombination occurrence. I found many antibodies for this but could anyone kindly suggest a good one with a reasonable price, based on your experience? I normally purchase antibodies from Santa Cruz for my western blot but their anti-Cre antibody (sc-83398) has been discontinued.

Thank you in advance.

Best regards,

Trinh

I would like to buy commercial catalysts to use in a Fischer Tropsch reaction but haven't had any good luck. Does anyone know of commercial suppliers of catalysts for this reaction?

Thanks

Hello there my research gate family, hope you are all doing great. I am planning to acquire a sequencing machine and I am planning for oxford nanopore but before that, I want to conduct a financial analysis to estimate purchasing, management, reagents and operational costs so that I could reach a final decision.

Prior to that, I have no hands-on experience with that technology so I don't know how much expensive it could be to manage sequencing tasks.

So I need your help to estimate the purchasing costs, management costs, operational costs and reagents costs

Thanks.

Actually i want to purchase eccosorb (ls-24,ls 30) absorber material. And i want it on urgent basis....Kindly suggest me vendors who can supply it to banglore .

Ok going to try to ask the question differently - has anyone used an Oxford Nanopore MinION and what are the purchase and running costs and for what sort of sequencing?

As methanol is a highly polar solvent they generally produce polar compounds peak in gas chromatography. But we found some non-polar and volatile compounds also. For GC-MS analysis, we have used a slightly polar column (Rxi-5ms, 0.25 m, 30 m long, internal diameter: 0.32 mm). Further, there is no chance of contamination from chromatographic column even after subsequent sample analysis since we are routinely analyzed our sample after a several wash of column by the GC grade ultrapure solvent/mobile phase and ensured an acceptable baseline first. Moreover, after careful and sufficient washing, we are conditioned our column following the experimental method and carefully checked baseline for its acceptance; then we run the sample for analysis. After that, we also run the blank sample to avoid any kinds of associated contaminations and false identification of compounds in the analysis. Furthermore, we reported only those compounds which are probable compounds matched from our comprehensive library (purchased with the GC-MS instrument).

What are the reasons?

Thanks in advance for your comments and explanation.

What are your thoughts on the growing popularity of online degrees, such as online MBA and online DBA? Do you think there is an increasing trend of online degrees? Is it a good or bad idea to take an online degree program? Please explain.

I am currently considering to purchase the bench Top Mini high energy planetary ball mill. As I have never used it before, I need your perspectives on its suitability for synthesizing the above materials.

The attached image shows a metal cap (GL45 size, compatible with most 1 or 2 liter glass bottles) with a hole. It was bought by a guy previously worked here and no information was left. I need some more of this and I have been searching around general lab supply manufactors but not luck. I'd be greatly appreciate if anyone could share some information about where I can purchase it.

P. S., I just need the metal cap, not the septa or the black plastic cap.

Thanks in advance.

Im conducting a study where I want to investigate consumer attitudes and purchase intention via questionnaires. I know that product related attitudes (price, taste etc) affects purchase intention, but I'm interested in investigating other areas. Do I need to include these product related attitudes in my questionnaire and control for them in my analysis, or can i disregard/exclude these variables entirely from my study?

Thanks in advance.

Hello, I am going to purchase a nitrogen evaporator for the lab I manage today. My supervisor mentioned a blog that compares and reviews lab equipment because I mentioned I want to purchase a good product and not something from a disreputable company. I found LabCompare but it doesn't have reviews for the specific evaporator I am looking at (Organomation 15 position unheated evaporator). I also do not really trust manufacturer/supplier reviews because those are so easily faked by the company. Any info anyone could provide would be extremely helpful. TIA.

I would like to spin coat PET or Polyimide films (whichever is easiest and deals with the least toxic chemicals). I'm not sure where to look or what I should purchase.

I purchased Thermo Scientific Pierce Streptavidin- Horseradish peroxidase conjugated and in the product data sheet its explained that you can reconstitute it with NF water, but avoid repeated freezing and thawning and as i use 1 uL per essay i cannot make such smalls aliquots for that. That´s why I was looking for a buffer that could preserve my hrp ;) Thank you in advance

Here Consumer attitudes (X), Sales promotion tools (Y) and Purchasing behavior the outcome variable.

Dear colleagues,

Pleas help us on lab equipment question.

We plan to purchase ICP-OES (Thermo fisher Scientific) and proper package of element references will be needed.

Taking into account, that our field of interest includes all possible elements, particularly PTEs ( Ag, Al, B, Ba, Bi, Ca ,Cd, Co, Cr, Cu, Cs, Fe, Ga, K, Li, Mg, Mn, Mo ,Na, K, Ni, Pb, Rb,Sr, Se,Sn, Tl, Ti, Zn, V ) we need to understand what kind of references will be suitable for us. The main question is wether we need single RMs with Multy RMs or not, to avoid possible interferences of elements.

I am looking for a compound called Tryptoquivaline F/J (or Fumitremorgin F/J) for some validation assays. Either to purchase or synthesize. Thanks!

I am looking and need to purchase all variety of Dental Pathology cells and Histologist cells slides example dentin, bone, etc

Having a bit of trouble with working out some calculation's.

I have purchased 1mg PMA and plan to dissolve in 1ml DMSO to get a concentration of 1mg/ml.

My plan is to aliquot thESE and freeze. Perhaps 1ul in each vial?

However i am unsure of how many mls of media to add to each aliquot to give the final concentrations of 2.5ng/ml, 5ng/ml, 10ng/ml, 20ng/ml and 50ng/ml?

Probably quite a simple calculation but any help would be greatly appreciated!

Thank YOU

AACTGQ = The AIDS Clinical Trials Group Adherence Questionnaire

Any permissions or purchasing required to use this self-report measure?

Hi all, i would like to know what are some of the key considerations one has when it comes to purchasing metal powders for printing? Price point is definitely a consideration, how about quality? or even availability of powder sizes or availability of small batch like (10kg?) is that a challenge in purchase of powders? any other challenges when you face when it comes to purchasing metal powders?

I want to purchase some drug compounds from ZINC15 database. I wonder how to get a quotation to estimate the cost of selected compounds.

Thank you,

Amal.

My independent variable is a functional claim (Claim vs No Claim) and my dependent variable is purchase intentions that have been measured by five (05) questions on a Likert scale of 1-5. Now, I want to perform an independent sample t-test to check whether functional claim levels affect the purchase intentions or not. So, which values of purchase intention should I use? Either that computed from factor analysis in SPSS or just took a mean of five questions of purchase intention. If I take mean then the effect is significant, and if I take values of factor analysis then the effect is not significant. Please help.

If anyone has bought items how did you go for it, credit card or band transfer? How is the experience?

Hello everyone!

We are working on factors affecting consumer purchase intention of organic food products in Haryana state in India. We need help in framing the questionnaire for this purpose.

If you have a standardized questionnaire in this regard we will be appreciated it to share it here.

Thanks for your help and guidance.

Plzz help me. I find 2 articles only but my sir reject it

I am willing to do some kind experiments on Picrorhiza kurroa and for the same I need seeds.

The flow cell in our HPLC detector has been broken. We would like to replace it with a new one. However, is it possible to buy it?

Hi there!

I am working with the SCC-15 (CRL-1623) cell line that I purchased from ATCC. I carefully follow the protocol recommended at ATCC . However, the cells are growing very slowly. Is growth always slow? How can I accelerate growth?

The specification should be:

1. Reactor tank 5 litre.

2.UV lamps 100 W, 200 W, 300 W and 400 W.

3.lamps power supply.

4.lamp immersion well.

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can anyone weigh in on the quality and their experience? TIA

Hi! I am doing a quantitative study on the impact of aspects of online reviews on purchase intention. I have conducted a survey, which resulted in a valid sample of 180. This measured 4 concepts as independent variables (predictors), and 1 dependent variable (purchase intention), all using validated measures and a 5-point Likert scale for all questions.

I have calculated both the Pearson's correlation coefficient and the standardized beta coefficient using a multiple regression analysis. They are similar, but give a slightly different ranking for the 4 predictors.

Can someone tell me why they result in