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Purchasing - Science topic

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Hello everyone,
I would like to ask have anyone works on the HS-5 cell line? May I know how long it takes for the cells to confluence after revive? My HS-5 cell line is newly purchased. After revived, the cells not confluence after 2 weeks. Kindly advised on what should I do. Thank you.
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Thank you Zeyno Nuhoğlu Öztürk , I will try the recommendation.
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Hello,
As a part of my research work, I need to detect the wild type p53 level in MCF-7 cells using western blotting. For this purpose i would like to know which antibody should i purchase (i.e) either monoclonal antibody or polyclonal antibody. If it is mnoclonal antibody, will it have any effect if it is produced by immunizing the animal with recombinant human p53 protein expressed in E.coli? Further, if there is any cat log no: available for the product (p53 antibody), i kindly request you to provide. Please do the needful.
Thanks in advance.
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Pierre Nsele Mutantu Thank you for your input. But this particular antibody is not synthesized by us. Rather it is purchased from outside and there are only restricted no: of monoclonal antibodies available. Out of 2-3 antibodies, i thought that this particular antibody will be a better choice. I have also given the details of the antibody below. Kindly check it out and please let me know whether i can proceed with this particular antibody.
P53 ANTIBODY DETAILS:
Specificity / Sensitivity
p53 (DO-7) Mouse mAb recognizes endogenous levels of total p53 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human p53 protein expressed in E. coli.
Thank you,
S.K.Janani
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I want to create multi-layer biomedical scaffolds and i need graphene or graphite sheets.
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Hi Sofia,
You may make an inquiry at Alfa Chemistry, they offer kinds of good-quality materials.
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I am just wondering which theory best describes moderating role of price conciseness between purchase intentions and behavior?
Arif
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Hello Arif
I have research about this issue. And I recognize that we should consider how much the consumer charge for a product. I research it in
psychological context. I think the most helpful approach is Anchoring Theory. Maybe my experimental research could help you about it:
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I am working in a paper as I need a dataset for customer purchasing products from a market. where can I find it ?
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I suggest you to get help in LinkedIn and ask people to like your post, so this way more people can see your post
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Dear fellow researchers,
I have been looking to buy some updated database for Raman spectroscopy of different organic, inorganic minerals, polymers etc., (updated with new entries at least in 2020). Could any of you suggest some sellers or links for the same.
Thank you all in advance.
Best regards
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hey.. please check out
but only limited to inorganic materials, not for polymers...
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i came across the various kits avalable for AKP activity in mammalian system. But i want to check AKP activity in group of LAB. WIll the same kit works for the same or i need to purchase other one? Also can we check the activity manually? Is there any protocol avalable?
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Thank you Rani and Kashif. I have gone through the protocol but i am getting my AKP activity in pellet instead of supernantent. Even i tried Triton X-100 and again sonicate cell suspension. But to my surprise no activity was in supernatent. One possibility is, as it is a membrane bound enzyme (i am guessing tightly bound) and it is not getting detached upon sonication. however research papers shows they had done it without using any detergent and only sonication serves the purpose. Can you suggest what should i need to do. thanks in advance
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I'm looking to purchase premium plagiarism detection software. Which one is the most reliable and accurate on the market?
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Dear RG colleagues,
This respected portal (i.e. RG) defines plagiarism as: The term “plagiarism” has different meanings, but it usually includes copying somebody else’s work without permission.
I may be somewhat old-fashioned, but please have the following golden rules on how to avoid plagiarism, especially Self-Plagiarism:
  1. Use your own words instead of copying the words of others. Needless to say that if you are using your own words, then there is no chance of plagiarism accusing.
  2. If you have co-authors, just trust your words.
  3. If you use your own words, there should be no plagiarism issue. In turn, there is no need for the tools of plagiarism checking. Since there is no guarantee that the original content of your manuscript might not be copied and sold to others before it is published by you, I discourage using any free-software checkers for plagiarism; some of them are betrayers. Despite that offline ones are rare and if you are insisting to use anti-plagiarism software, offline checker programs are safer than online ones.
  4. In some cases, you can paraphrase the sentences in the original document. But don't forget to write a reference.
  5. You must always insist on honesty.
  6. You must always insist on doing real research, not "Wikipedia" research.
  7. Do not put any of your research work anywhere until it is published and tagged with your name. Please wait until the paper is accepted and then published in that journal. Then, upload that research item on any platform you wish.
  8. Despite that offline ones are rare and if you are insisting to use anti-plagiarism software, offline checker programs are safer than online ones.
  9. In my opinion, most of the free-software-checkers for plagiarism don't work effectively. Unfortunately, you have to pay for the sake of getting good results.
Finally, believe me, or not: If you make one plagiarizing, you may solve one problem and fall into many others where some of which may be described as a knockout. Again and again, please always remember that if there were accusations of plagiarism, it is not well for any researcher's reputation, in any meaning.
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Usually for plagiarism checking Turnitin software is used, that is not freely available. Is there any plagiarism checker software that can freely available or can be used without purchase?
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Dear respected colleagues,
Let me point to another critical issue related to plagiarism software-detectors. My friend was accused of plagiarism. Why?
After about five months, his promotion process to associate professor was rejected because of plagiarism. When he checked the promotion report, he found that one of his papers was accused of plagiarism and with a 100% percentage. The reason is that his manuscript was checked by his co-author using one of these checkers. It took him several months time of following up to solve the problem and removing the manuscript from their database. After that long period, he re-apply the promotion order for the second time.
Therefore, plagiarism software may retain a copy of the manuscript in its database. To state the truth, this is depending upon the settings and type of account subscription.
So, you may face a similar situation when you submit your manuscript after plagiarism checking to a journal. It may be rejected instantly because it would show up 100% similarity index.
To solve the problem, it may take several months time of following up and removing the manuscript from their database.
If there were accusations of plagiarism, it is not well for your reputation, in any meaning.
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This is my first introduction to statistical analysis. I am wanting to understand how to test the theory that influencer marketing will drive sales. Two yes/no questions were asked and answered including "Do you follow any influencers?" and "Have you ever purchased anything seen used by an influencer?" From the outset the data seems to show that those who do follow influencers are inspired to purchase. But it also appears to identify that those who do not follow are also influenced. I'm really trying to work out the best formulas for analysis. If anyone could help, it would be greatly appreciated.
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As practical advice, be sure you don't place too much importance on the p-value from a statistical analysis. A hypothesis test, like the chi-square tests of association mentioned in some other answers, essentially indicates if there is enough evidence of there being a "signal" in the data relative to the "noise" in the data. This is interesting, but doesn't tell the whole story.
As mentioned in another answer, you want to think about the size of the effect you are considering. Odds ratio is a measure that is relatively intuitive. For a 2 x 2 table of counts you can also use the phi statistic, which is analogous to the correlation between the two variables (on the axes of the table of counts).
It's often most meaningful to present the actual data in a plot or table. In this case, you might present the counts in a table, or these counts as proportions (perhaps by row or column, if that makes the most sense).
A bar plot of these counts or proportions can be meaningful ( ex. https://rcompanion.org/handbook/images/image319.png ).
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I'm hoping to identify microbleeds in the brain tissue of a rat, so thought to stain for iron. I have perfuse-fixed frozen tissue on slides in 10um sections and I bought Prussian blue but would love some advice or an existing protocol - how best to make up the solution, prepare the tissue and times/temperatures to stain it!
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Has anyone had any success using Prussian Blue staining on frozen tissue sections?
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Recently, my UG students analyzed a protein for drug binding site on DogSiteScorer. Can we published this data by citing the website or we have have to purchase the license?
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no
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Public procurement denotes the attainment of goods, works, or services required by governments through contracts. Public procurement is usually plagued with covert practices and exclusive forms of corruption in Bangladesh. The cost of development finance is much higher in public procurement than that of others in Bangladesh. There have been no unified rules or laws regarding government purchases since 2006. The Government of Bangladesh enacted its ever first act of public procurement, the Public Procurement Act, 2006 (PPA, 2006), and the Public Procurement Rules, 2008 (PPR, 2008). The procurement entity must disseminate procurement information through electronic medium i.e. Electronic Government Procurement (hereinafter e-GP). Despite passing over an era since its enactment, the role of the PPA, 2006 remains seriously under-researched area, specifically there is no research on the issue of the grievance redress mechanisms. The study has been done using a qualitative case study methodology, backed by both primary and secondary documentary analysis. Based on empirical data, this paper explained the role of digitalization in the process of government procurement and depicted changes that have been brought by the implementation of e-GP in government purchases. However, it concluded that the grievance redress mechanism in the process of procurement is only existing in the policy papers which need mandatory implementation for a transparent and accountable governance system. Despite the significant changes and up-gradation in digital government procurement, it is hardly found that the GRM process is digitalized. And the mass tenderers are yet to be acquainted with the improvised system apart from the consistent efforts of the government.
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It is important suggestion to think more critically.
Best regards
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Hi.
We are working on tree growth/health enhancements and were wondering what sort of bacteria or fungi (or a consortium thereof) biostimulants or biofertilizers (plant growth/health promoting bacteria or fungi) are available for purchase in Europe (i.e. comply with EU standards)? Can anyone help?
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Look at Mack-Agrar they have mykorrhizae cultured on living hosts
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I am looking to purchase a Laser micromachining system for fabricating microfluidics devices with 35micron rounded channels from PDMS, PMMA or glass. What is the best make and model to achieve this type of feature resolution and what system parameters are the most important ones that I should be aware of? Thank you
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Standard CO2 lasers are cheap but not easy to use to produce round channel with precises dimention. Femto laser like one from https://www.femtoprint.ch/ can be use to produce channels into a specific glass material with a two step etching: 1. laser pre-condition glass and 2. channels are wet etched https://www.degruyter.com/document/doi/10.1515/nanoph-2017-0097/html
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I am just wondering which theory best describes moderating role of price conciseness between purchase intentions and behavior?
Arif
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I will propose to you the theory of planned behavior and the Elaboration likelihood model
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I am just wondering which theory best describes moderating role of price conciseness between purchase intentions and behavior?
regards
Arif
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I am having some issues with Bovine serum I have purchased and am wondering if there is a better serum to purchase that others have had better success using? any advice would be appreciated.
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Dear Victoria
If your biosensor is going to be used for human diagnostics, I would strongly recommend using normal human serum. Merck-Sigma Aldrich does sell this product.
One other point. If your biosensor is intended for use on whole blood, you may need to test in normal plasma as well to find out any interaction with large plasma proteins such as fibrinogen.
Good luck.
Siva
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I am conducting a customer satisfaction survey on visiting and purchasing customers of a shopping mall, therefore no database to understand the population. As I am conducting the research targeting only visiting customers, from day to day numbers are different. So can this be an infinite population? can I use G power to calculate sample size? or any other method to identify the population? Thank you so much
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In a customer satisfaction, the population is necessarily finite, since there are no "infinite customers".
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Hello,
I have purchased MIN6 and INS-1E cell lines (Beta cell) from AddexBio and I'm culturing them in flask based on the protocol they provided. However, for some of my tests, I have to culture them in 6 and 12 well plates. But 3 days after plating, they start to round-up and lose the normal protrusions that they have while attaching to the surface. I know that the round-up cells are not dead.
this pattern starts from a corner of well and goes forwards towards the center of well. I have changed my well-plate plastic material, culture media, incubator, etc. and this problem is still there.
I should mention they look normal in flask but once I plate them in well-plates, I observe this issue.
So I wanted to ask if you have any explanation or suggestion to help me with this issue? I would be thankful for your comments.
I have attached here an image showing how they look like.
Thank you.
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Hi Jimmy, DMEM from Sigma (D6171), and I would change the media once on the 3rd day after splitting the flasks. I would split the cells approx once per 5-6 days. Not any more using MIN6 though.
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Hello, I need to recruit respondents for my PhD research survey. Is there any reputable place to purchase or rent an email list?
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Hi Shaima,
Here and at the bottom are some Australian-based companies' links, you just browse them to find more information regarding email databases/lists, whether buying or renting, good luck.
Best wishes
Sofiane
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Currently I'm working on a project related surface ship simulation. We need to simulate a plenty of ship types. I modeled propeller, rudder etc. But hydrodynamic forces are modeled as hyrodynamic derivatives (hyrodynamic coefficients, maneuvering coefficients ). For some ships, we generate our hyrodynamics data, but as I said, we need lot more. We dont want to spend time on CFD. We need direct data of Hydrodynamic of some ships. Is there such a company/institute/university/organization etc.? Any suggestion?
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Follow the references [14, 35, 36] mentioned in the following manuscript for coefficients of DTMB 5415, PCC, and VLCC ships
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Hello
I need poly SIBS for my PhD project because of its suitability for medical metal coating. However I can't find commercially available poly-SIBS. I contacted with K.neka corporation in Japan but they didn't want to send the poly SIBS sample for medical use. Even though it's not for medical usage, only for academic purposes I couldn't find it anywhere. The only product I could find is poly SEBS from Fisher which doesn't include exactly the same chemistry as SIBS.
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Hello everyone,
I would like to know how other battery groups store their In foil. It's the first time I purchased it and the Sigma package came totally not airtight like for Li or Na and just in regular packaging. Is that normal? Thanks.
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Metallic Lithium is reactive for the air (H2O). It forms easily LiOH and releases H2 gas when it contacts with H2O. It needs to be stored in vacuum or Argon gas environment. Other battery materials (oxides or carbon-based) in powder forms are generally stable in open air. However, they need to be heated in vacuum oven at 80-120 C overnight to remove adsorbed H2O before using them for slurry preparation.
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I am really confused with the variables that we will use in our study. We aim to identify the direct influence of consumer attitude toward pop-up ads on their purchase intention. In our framwork, the purchase intention is the dependent variable. However, the consumer attitude itself can be influenced by pop-up ads, so I believe that it cannot be labeled as the 'independent variable', since it depends on the pop-up ads.
My question is, what type of variable is the attitude, as well as the pop-up ads, if our main focus in the study is the attitude and purchase intention?
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I would say it depends how you want to use this variable of attitude in your model and interpretation. Is it a covariate you are controlling for? Is it a possible moderator or mediator? You may even want to run additional analysis stratifying subjects by attitude parameter. These are important considerations before you build your model. If you just want to control for attitude and effectively regress it out, these would typically be considered covariates of no interest.
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Hello,
I am currently writing my bacherlor's thesis and I am now at my results part. However, I am not quite sure which test I should use now to answer my hypothesis, two-way ANOVA or multiple lineair regression?
My hypothesis are
H1: Purchase intention is higher for advertisements that use human influencers (vs. virtual).
H2: Self-esteem will moderate the effect of the type of influencer on purchase intention.
In which self-esteem and type of influencer (human vs virtual) are the independent variables and purchase intention is the dependent variable.
Thank you.
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For H1, you have one categorical independent variable, so I guess you should go with one-way ANOVA.
For your H2, I'm not sure. Do your virtual influencers also have self-esteem?
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Hi everyone
I want to study the role women consumers play in the purchase decision-making process. Marketing literature states 5 roles( initiator, information search...) Since these are on a nominal scale, I am finding it difficult to build my model.
Can anyone suggest a scale which can be used to measure the same?
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i dont recommed meausuring consumer choice with a pre established scale
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I wanted to make triethanolamine oleate as a lubricant.
So I purchased oleic acid. There were two different oleic acid (industrial grade) in the maket: liquid and semi-solid.
What is their difference?
I used liquid as trusted and good quality. I mixed them together without any catalyst or pressure-temperature conditions and the result was unsatisfactory.
What is suitable condition for this esterification?
Thanks
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I have same problem oleic acid didnot react with amine base and i read a lot but no one mention detailed procedure
Any help plz ??
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I am looking for patterned ITO substrates for ready purchase to make my solar cells. Can anyone let me know the details of any Indian agent?
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I want to know where you got cell lines if you recently purchased MH7A cells. Thanks
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I am not sure to understand your question. Did you mean "where is it possible to purchase MH7A?". If this is the case you can see:
that the only cell line collection distributing MH7A is RCB (Riken).
Best
Amos
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Dear all,
Greetings.
I am working on my PhD where I am exploring the role of women in purchase decision-making w.r.t Consumer electronics. It is an interdisciplinary study where I have studied and combined decision-making aspects from the literature on women's empowerment and role in purchase decision-making as established in marketing.
My framework includes testing the impact of 3 demographic factors (Age, Education and work status- IDV, nominal scale) on Consumer Self-Confidence, which is having 5 constructs (DV- Likert scale).
The outcome- what role women have in decision-making.
It is believed, that more the Confidence, the more should be the role in decision-making.
I am seeking guidance from experts to understand what software and tools will be best to study the above state? IDV are nominal, DV are Likert scale and outcome is again nominal.
Any suggestions for betterment are welcome :-)
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Thanks Ashraf. Yes SPSS is applicable for hypothesis testing. However haven't seen python in marketing studies. I shall explore. Thank you for your time.
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For my research I'm trying to show how/whether or not trust mediates the relationship between anthropomorphised online agents and purchase intention. I've used existing scales to measure these variables but I'm not sure how this is done in SPSS - do I need to create a new single variable for anthropomorphism, trust and purchase intent combining the questions from the scales, or do I need to do something else?
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I would strongly recommend factor analysis over principal component analysis, due to the different assumptions they make.
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In the attached photos you can see that when I put eigenvalue higher than 1 (which is of course supposed to be) I get 6 components, however my conceptual model proposed 7. For illustration I put in eigenvalue higher than 0.976 to ask the following question: is it possible to get this number to 1? Right now with 6 components, attitude and purchase intention are on the same component however in my conceptual model attitude leads to a higher purchase intention. Is this solvable?
Kind regards,
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First of all, it appears that what you did was principle component analysis (PCA), not factor analysis. Second, the eigenvalue > 1 criterion is not typically extremely useful. Third, varimax (i.e., orthogonal) rotation is often not the best choice because it leads to uncorrelated factors/components, which is often not plausible. You can refer to the paper below for the reasons why FA is likely to be a better choice for you than PCA, why the eigenvalue criterion can be problematic, and why varimax rotation should be avoided.
Note that you can simply force the number of factors to be retained to be a certain number that is in line with your theory. In your example, you could simply ask SPSS to extract 7, rather than 6, factors. Then, you can examine whether the rotated loading matrix (oblique rotation is generally preferred) is in line with your theoretical expectations.
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what methodology is recommended to show the relationship between the consumers purchasing behavior with their demographic nature??
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Used survey research design and employed correlation to determine the relationship.
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Hello. I am trying to perform cell dissociation for the whole adult mouse brains (pnd90). I used Worthington Papain Dissociation System for a while, but after dissociation, cell viability was too low and the kit is a little too expensive for me to purchase it constantly. Does anyone have a cheaper protocol using media such as Hibernate, and enzymes like Papain with Dnase etc.? How much papain and Dnase would be enough to dissociate cells of a whole adult mouse brain and how long should papain incubation be in this case, according to your experiences? Thanks in advance.
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Were you ever able to successfully dissociate from the adult brain? I am currently working on a similar papain-based protocol in another species and have been getting weak results as well
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A link for purchase would be helpful!
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Hi Amogh,
You may make an inquiry at Alfa Chemistry, they offer kinds of good-quality chemicals.
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Online purchasing has become the fastest and easy mode for household product purchases around the globe. It reaches your home according to your specified time frame.
Do you think that the products we buy are value added as when we purchase through a showroom we get the advantage of a real time demonstration of the product with various advantages and disadvantages comparison with the other products and the representatives of the companies pulling us to buy their products providing us instant GIFT coupons and offers to attract them.
What I am trying to explain is the advantages and disadvantages of buying a household product from a showroom Vs online.
Do you think that this opportunity of seeing and buying from the showroom is better than reviewing the products online and buying the product.
Lets have a serious discussion on this topic.
Thanks,
Nujum Niyaz
Lincoln University
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In using online shopping , Customer is king but Customer has to choose between what is shown to him on that line . Complete information may not be revealed .
This can lead to information asymmetry and shopping of product which is based on Market need and not on real Customer need..
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Our lab purchased the Spectramax ID3 and have encountered an error in the built in computer due to an issue connecting to the hard drive. Has anyone had experience with this and know of a resolution or the cost of repair?
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We are measuring "Purchase intention" and have therefore made a framework where Four variables, Involvement, Argument Quality, Source credibility and Information Usefulness together make up Purchase intentions.
We have conducted a survey with a number of questions for each variable mentioned above with an answering scale of 1-6. The aim of the study is to compare the mean of each variable inorder to see if the two groups (Genz & Millenials) the survey was sent out to differentiate in some way. We are also wondering how to "combine" these four variables inorder to measure the mean for Purchase intention for each group.
Thanks in advance
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If your goal is to compare mean scores between the two groups, then you can just use a series of t-Tests. If you want to determine which variables predict the group a person belongs to, based on comparing the full set of variables, then you can use either discriminant analysis or logistic regression.
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We need to purchase a paraffin embedder and cold block. Please send me reviews on your experience with specific model numbers. New vs refurbished? Companies/models to focus/stay away from. Thank you in advance!
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Matthew Ibbs Thank you for taking the time to let me know your experience. I appreciate it!
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I am planning to work with interstitial fluid (ISF). Is there any place that I can buy normal ISF? or, Can you please suggest the composition to make in the lab? or, Is there any convenient method to extract and use it?
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Hello Laxman,
I see this question is old, however I will answer in case you are still interested.
I am not aware of any commercially available artificial ISF. However, I'll first point you to some articles from a couple of other groups that have prepared artificial ISF
  • Biosensors and Bioelectronics 123 (2019) 152-159
  • Electrochem commun. 2017 September; 82: 1-5. doi: 10.1016/j.elecom.2017.07.011
I'd also point you towards some of our lab's publications (which you can find on my ResearchGate page) in which we have started to measure the metabolic, proteomic, and transcriptomic composition of ISF.
Best regards,
Robert
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Does anyone know a source where I can purchase it premade or if you have a spare one laying around and willing to donate?It is a microdot stamp to create microisland cultures of astrocytes and autaptic neurons.
Please help
Sam
#astrocytes
#neurons
#synapse
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Hi Sam did you find one? We would like to purchase one too! Thank you!
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It's my humble request to Research Scholars.
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Hi Hitendra
You may make an inquiry at Alfa Chemistry, they offer kinds of good-quality chemicals at a reasonable price.
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I want to collect data and analyze it using AMTI force plates. I do not have Visual3D purchased. Any leads would help.
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Hi Ajay,
- AccuPower is suitable if you are working on athletic performance evaluation.
- AMTI’s BioAnalysis software package is a complete analysis suite ideal for gait, balance, and power analysis.
A file containing additional information on either of the above-mentioned software is included.
Good luck
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Dear all,
Can anyone suggest me a good plasmid with Tetracycline resistant gene to purchase ? (resistant only to tetracycline)
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Dear Gayathri,
Since you didn't mention the purpose of the plasmid, please visit addgene website where you can choose and order from a wide variety.
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We have a quantitative research study title of "The Effects of the COVID-19 Pandemic on the Online Purchasing Behavior of Senior High School Students".
In this study, our goal is to determine the effects as well as certain factors that make up for the changes in online purchasing behavior due to the COVID-19 pandemic on senior high school students.
What I would like to ask is what would our quantitative research design would be: descriptive, correlational, ex post facto, quasi-experimental, or experimental?
I would appreciate your thoughts and suggestions, thank you.
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You may consider the TPB model or the UTAUT model explaining online purchase intention among students. Also, mediators should be considered, such as satisfaction or brand image. The COVID-19 pandemic could be considered as a moderator.
All the best,
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Both the ELISA and another testing method will work.
I sent a purchase request to IGM Bioscience (Palo Alto, USA), but did not get in touch. Thanks.
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Dear Regina,
This type of products are available only as customer service:
For more details about how to order these produts - you can contact me.
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I am currently working on a money supply simulation and there are some pieces of the puzzle that are still missing. One of them has to do with the purchase program of the ECB.
Say I am a pension fund which is holding a government bond which the ECB wants to purchase for 1000 EUR. With the purchase program the ECB creates money to purchase this bond but that money is digital central bank money which can not be transferred to my account. My question is, where does the money that I eventually receive om my account come from? How would that look when explained with the balance sheets of me (the pension fund), my bank and the ECB?
Thanks in advance,
Stef Kuypers
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Hi Stef
I have prepared a document with hypothetical changes in the Balance Sheets of the Pension Fund, Central Bank and the Commercial Bank. Please see if it helps. Do let me know if there is some error.
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Hello everyone,
I am currently want to confirm the production of Cre recombinase by Western blot in my cells after infected with the Lenti-Cre virus since we did not see the recombination occurrence. I found many antibodies for this but could anyone kindly suggest a good one with a reasonable price, based on your experience? I normally purchase antibodies from Santa Cruz for my western blot but their anti-Cre antibody (sc-83398) has been discontinued.
Thank you in advance.
Best regards,
Trinh
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Thank you, Abdulla and Nikhil for your kind suggestion. I am considering buying the anti-Cre from Biolegend but will have a look at some references using the one from Thermofisher to make the decision.
Truly appreciate your response.
Best regards,
Trinh
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I would like to buy commercial catalysts to use in a Fischer Tropsch reaction but haven't had any good luck. Does anyone know of commercial suppliers of catalysts for this reaction?
Thanks
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Dear Aroldo José Romero-Anaya many thanks for posting this interesting technical question on RG. I'm not a proven specialist in heterogeneous catalysis, and I'm not going to name yet another commercial supplier. According to the rather instructive Wikipedia entry at https://en.wikipedia.org/wiki/Fischer–Tropsch_process the Fischer-Tropsch reaction is best catalyzed by molybdenum carbide as well as by cobalt-, iron- and ruthenium-based catalysts. My suggestion would be to first decide which metal(s) you want to employ (after reading one or two reslevant review articles) and than search for commercial suppliers of the indvidual catalysts.
Good luck with your work!
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Hello there my research gate family, hope you are all doing great. I am planning to acquire a sequencing machine and I am planning for oxford nanopore but before that, I want to conduct a financial analysis to estimate purchasing, management, reagents and operational costs so that I could reach a final decision.
Prior to that, I have no hands-on experience with that technology so I don't know how much expensive it could be to manage sequencing tasks.
So I need your help to estimate the purchasing costs, management costs, operational costs and reagents costs
Thanks.
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Thank you both for your suggestions.
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Actually i want to purchase eccosorb (ls-24,ls 30) absorber material. And i want it on urgent basis....Kindly suggest me vendors who can supply it to banglore .
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Hi Navyasri,
You may make an inquiry at Alfa Chemistry, they offer kinds of good-quality materials.
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Ok going to try to ask the question differently - has anyone used an Oxford Nanopore MinION and what are the purchase and running costs and for what sort of sequencing?
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Hi Simon N Waddington . How was your experience with ONT? Did you find minion useful?
Best,
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As methanol is a highly polar solvent they generally produce polar compounds peak in gas chromatography. But we found some non-polar and volatile compounds also. For GC-MS analysis, we have used a slightly polar column (Rxi-5ms, 0.25 m, 30 m long, internal diameter: 0.32 mm). Further, there is no chance of contamination from chromatographic column even after subsequent sample analysis since we are routinely analyzed our sample after a several wash of column by the GC grade ultrapure solvent/mobile phase and ensured an acceptable baseline first. Moreover, after careful and sufficient washing, we are conditioned our column following the experimental method and carefully checked baseline for its acceptance; then we run the sample for analysis. After that, we also run the blank sample to avoid any kinds of associated contaminations and false identification of compounds in the analysis. Furthermore, we reported only those compounds which are probable compounds matched from our comprehensive library (purchased with the GC-MS instrument).
What are the reasons?
Thanks in advance for your comments and explanation.
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You should not use GC-MS but LC-MS for qualitative and quantitative analyses of a methanol extract which contain mostly polar compounds. Minute amount of non polar compounds may be present in a methanol extract. You have to bake the column at high temperature for a few hours or overnight to clean the GC column. We use a liquid mobile phase to wash a LC column.
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What are your thoughts on the growing popularity of online degrees, such as online MBA and online DBA? Do you think there is an increasing trend of online degrees? Is it a good or bad idea to take an online degree program? Please explain.
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I agree with the point of view of Prof. Ingrid Del Valle García Carreño
In addition as a secondary degree for non-digital natives is great, particularly a master's degree in new LMS technologies and learning and teaching techniques, Prof. Pongsakorn Limna
I had the pleasure to ta take for free a diploma in the design of virtual learning environments sponsored by foundation Carlos Slim in Mexico City, it was great for me.
Best Regards.
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I am currently considering to purchase the bench Top Mini high energy planetary ball mill. As I have never used it before, I need your perspectives on its suitability for synthesizing the above materials.
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Thank you for your contribution. the paper you shared was of great help.
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The attached image shows a metal cap (GL45 size, compatible with most 1 or 2 liter glass bottles) with a hole. It was bought by a guy previously worked here and no information was left. I need some more of this and I have been searching around general lab supply manufactors but not luck. I'd be greatly appreciate if anyone could share some information about where I can purchase it.
P. S., I just need the metal cap, not the septa or the black plastic cap.
Thanks in advance.
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They offer 10 pcs for 19.4 EUR.
I hope this helps you, have a nice day.
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Im conducting a study where I want to investigate consumer attitudes and purchase intention via questionnaires. I know that product related attitudes (price, taste etc) affects purchase intention, but I'm interested in investigating other areas. Do I need to include these product related attitudes in my questionnaire and control for them in my analysis, or can i disregard/exclude these variables entirely from my study?
Thanks in advance.
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We already know that control variables seek to rule out alternative answers to those sought in the study. They are factors that influence the dependent and independent variables but are not the object of the study. The differentiating aspect of control variables is that they are considered alien to the model, unrelated to the hypotheses being tested (SPECTOR; BRANNICK, 2011). However, many studies include control variables with no specific theoretical interest, sometimes without a clear justification for their use or present reduced comments. Given this context, I suggest pondering whether there is a theoretical and methodological assumption that justifies the need for the inclusion of these variables.
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Hello, I am going to purchase a nitrogen evaporator for the lab I manage today. My supervisor mentioned a blog that compares and reviews lab equipment because I mentioned I want to purchase a good product and not something from a disreputable company. I found LabCompare but it doesn't have reviews for the specific evaporator I am looking at (Organomation 15 position unheated evaporator). I also do not really trust manufacturer/supplier reviews because those are so easily faked by the company. Any info anyone could provide would be extremely helpful. TIA.
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Anoop@ apart from it, after sale service is very important criteria.
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I would like to spin coat PET or Polyimide films (whichever is easiest and deals with the least toxic chemicals). I'm not sure where to look or what I should purchase.
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Adam Weidling May I ask the curing process of PI-2611 you used? I tried one method that worked on PI2500 series, but not worked on PI2611--my PDMS was bent with PI 2611
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I purchased Thermo Scientific Pierce Streptavidin- Horseradish peroxidase conjugated and in the product data sheet its explained that you can reconstitute it with NF water, but avoid repeated freezing and thawning and as i use 1 uL per essay i cannot make such smalls aliquots for that. That´s why I was looking for a buffer that could preserve my hrp ;) Thank you in advance
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1uL per assay is such a small volume that can introduce large error in your assay. After reconstitution with NF water, make an intermediate dilution of the HRP-Streptavidin in phosphate buffered or Tris buffered saline. pH 7.4 containing 10mg/mL BSA ( for example 1/10dilution or 1/100 dilution then use it in your assay at 10uL or 100uL volume). I have kept streptavidin-HRP solution diluted in the above buffer for over 3 years at 4oC without loss of activity. Make sure you do not use sodium azide as preservative as it inhibits HRP enzyme activity. I recommend using Proclin 300 (Sigma-Aldrich) at 1/1000 dilution.
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Here Consumer attitudes (X), Sales promotion tools (Y) and Purchasing behavior the outcome variable.
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Likert scale items are measured with ordinal scale and the statistical tool that can test the relationship between Y and Z is Spearman rank correlation. Then with a mediating variable X we use partial correlation to examine the effect of X on the relationship between Y and Z.
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Dear colleagues,
Pleas help us on lab equipment question.
We plan to purchase ICP-OES (Thermo fisher Scientific) and proper package of element references will be needed.
Taking into account, that our field of interest includes all possible elements, particularly PTEs ( Ag, Al, B, Ba, Bi, Ca ,Cd, Co, Cr, Cu, Cs, Fe, Ga, K, Li, Mg, Mn, Mo ,Na, K, Ni, Pb, Rb,Sr, Se,Sn, Tl, Ti, Zn, V ) we need to understand what kind of references will be suitable for us. The main question is wether we need single RMs with Multy RMs or not, to avoid possible interferences of elements.
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Without knowing your particular sample type and expected concentrations, I would suggest getting a reasonably high concentration that can be diluted to your final working calibration. It is much harder (impossible?) to concentrate a 10 ppm stock up to 1000 ppm, but easy to dilute a 1000ppm stock to 10ppm.
If you can get several multi-mixes that would be good, but if you can only get one multi-mix and supplement it with mono-mix stocks that you combine yourself, then that will also suffice.
There are suppliers other than the manufacturers, try some of the larger scientific suppliers in your region. (I won't mention any names in this forum).
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I am looking for a compound called Tryptoquivaline F/J (or Fumitremorgin F/J) for some validation assays. Either to purchase or synthesize. Thanks!
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Hi Patrícia,
You may not find this compound easily, you may have to synthesize it. Or better get it done by a custom synthesis providing company. Have a look at - https://synthinkchemicals.com/
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I am looking and need to purchase all variety of Dental Pathology cells and Histologist cells slides example dentin, bone, etc
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k,
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Having a bit of trouble with working out some calculation's.
I have purchased 1mg PMA and plan to dissolve in 1ml DMSO to get a concentration of 1mg/ml.
My plan is to aliquot thESE and freeze. Perhaps 1ul in each vial?
However i am unsure of how many mls of media to add to each aliquot to give the final concentrations of 2.5ng/ml, 5ng/ml, 10ng/ml, 20ng/ml and 50ng/ml?
Probably quite a simple calculation but any help would be greatly appreciated!
Thank YOU
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Louise Dolan you're welcome.
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AACTGQ = The AIDS Clinical Trials Group Adherence Questionnaire
Any permissions or purchasing required to use this self-report measure?
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Hi, David Horton . The Adult AIDS Clinical Trials Group, which has contributed a lot to the global effort to control the AIDS pandemic, has been receiving substantial public funding since its inception that I find it unlikely for it to withhold its tools through strict permission and/or purchase/licensing schemes. Nevertheless, you can directly ask them for such concerns in this link: https://actgnetwork.org/contact/.
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Hi all, i would like to know what are some of the key considerations one has when it comes to purchasing metal powders for printing? Price point is definitely a consideration, how about quality? or even availability of powder sizes or availability of small batch like (10kg?) is that a challenge in purchase of powders? any other challenges when you face when it comes to purchasing metal powders?
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Actually, Metal powders used in AM processes are typically microscopic in size (< 100 µm) and often pose toxicity, reactivity, combustibility and instability hazards. Dust clouds, formed for example by the accidental swirling of powders, have the potential to catch fire and explode under certain conditions.
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I want to purchase some drug compounds from ZINC15 database. I wonder how to get a quotation to estimate the cost of selected compounds.
Thank you,
Amal.
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My independent variable is a functional claim (Claim vs No Claim) and my dependent variable is purchase intentions that have been measured by five (05) questions on a Likert scale of 1-5. Now, I want to perform an independent sample t-test to check whether functional claim levels affect the purchase intentions or not. So, which values of purchase intention should I use? Either that computed from factor analysis in SPSS or just took a mean of five questions of purchase intention. If I take mean then the effect is significant, and if I take values of factor analysis then the effect is not significant. Please help.
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Mohsin Iqbal Qazi You mention that your DV is measured by 5 Likert items. My first question would be, how well does a single-factor model fit these 5 items? In SPSS and other programs, you can extract a single factor EFA model with maximum likelihood (ML) estimation and obtain a chi-square test of model fit. Such a 1-factor EFA-ML model is equivalent to a single-factor congeneric confirmatory factor analysis (CFA) model, that is, a 1-factor CFA model with unequal factor loadings across items. If this model fits well (has a non-significant chi-square), then I do not see why you couldn't meaningfully use factors scores computed from this single-factor model (but see also my last paragraph about ordinal factor analysis).
The option of creating a simple (unweighted) mean score requires a stricter factor model to hold than the above 1-factor congeneric model. More specifically, the mean approach implies as an "assumption" that your items follow a 1-factor model of essential tau-equivalence (or stricter measurement models). The essential tau-equivalence model implies equal factor loadings across items. This can be tested by using CFA with constrained loadings, but is difficult to test with EFA. In other words, the mean approach implies a stronger assumption about the measurement properties of the items than does the congeneric EFA approach which allows for unequal item loadings (weights).
Another issue may be that your Likert items, strictly speaking, are only ordinal in scale level (not continuous/metrical). Therefore, a more appropriate approach may be an ordinal factor analysis. This is especially the case if the items are skewed (non-symmetrical in their distributions). Ordinal factor analysis is something that, to my knowledge, SPSS to date does not provide, but it can be done in software for structural equation modeling such as lavaan (in R) or Mplus.
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If anyone has bought items how did you go for it, credit card or band transfer? How is the experience?
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interested
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Hello everyone!
We are working on factors affecting consumer purchase intention of organic food products in Haryana state in India. We need help in framing the questionnaire for this purpose.
If you have a standardized questionnaire in this regard we will be appreciated it to share it here.
Thanks for your help and guidance.
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I am willing to do some kind experiments on Picrorhiza kurroa and for the same I need seeds.
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Kew Gardens may have some :)
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The flow cell in our HPLC detector has been broken. We would like to replace it with a new one. However, is it possible to buy it?
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Hi Dawid,
Yes, of course, you can replace only the flow cell for ProStar 330 Photodiode array detector, if you have the spare part, or you can order it which is a cheaper option instead of changing the whole detector.
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Hi there!
I am working with the SCC-15 (CRL-1623) cell line that I purchased from ATCC. I carefully follow the protocol recommended at ATCC . However, the cells are growing very slowly. Is growth always slow? How can I accelerate growth?
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Tongue Squamous Cell Carcinoma (SCC) is one of the most common cancer types, with a survival rate of less than 5 years in half of newly diagnosed patients. Nearly half of patients who are at the stage of diagnosis already have regional lymph node metastasis.
Check..
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The specification should be:
1. Reactor tank 5 litre.
2.UV lamps 100 W, 200 W, 300 W and 400 W.
3.lamps power supply.
4.lamp immersion well.
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Kindly visit..
Amazon or Flipkart
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I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can anyone weigh in on the quality and their experience? TIA
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نعم يوجد بعض الاشخاص لديهم خبرة في هذا المجال
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Hi! I am doing a quantitative study on the impact of aspects of online reviews on purchase intention. I have conducted a survey, which resulted in a valid sample of 180. This measured 4 concepts as independent variables (predictors), and 1 dependent variable (purchase intention), all using validated measures and a 5-point Likert scale for all questions.
I have calculated both the Pearson's correlation coefficient and the standardized beta coefficient using a multiple regression analysis. They are similar, but give a slightly different ranking for the 4 predictors.
Can someone tell me why they result in