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I have purchased ellagic acid from himedia, but when i perform antimicrobial assay by agar well diffusion method, it's not showing antimicrobial activity at any concentrations. I have use 1mg/ml, 2mg /ml and 3mg /ml concentration for testing against bacillus subtilis, pseudomonas aeruginosa, and S. aureus. Why i am not getting any results?
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I am going to take a different approach to the answer:
First, are you sure that ellagic acid is antibacterial? The reports I noted were not studies, and the study I looked at did not even discuss how ellagic acid was dissolved. If dissolved in a solvent (be careful with this suggestion), the solvent could be the actual cause of the "antibacterial" effect. If you use a solvent other than water, make sure you use a control of only the solvent to determine if it is the cause of the antibacterial activity.
Second, a simple mistake that I have even seen in posters is that the bacteria is spread on the agar surface, allowed to grow, and then the test material is added. Of course since growth already occurred the test material can not then prevent it from growing.
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I am trying to run a sulfatase activity assay using purified steroid sulfatase enzyme and 4-MUS as a substrate. The protocol I am following says to use 0.6 units of enzyme however the purified enzyme I have purchased did not come with a specified enzymatic activity (units). I'm not sure if this is a known figure or if I have to calculate this? any help is appreciated.
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In the absence of sufficient information on the specific activity of the protein you purchased, you could do a simple experiment in which you measure the product formation at various enzyme concentrations to find the concentration that gives you the activity you need.
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To measure The Impact of YouTube Advertisement Value on Purchase Intention of YouTube Users
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Questionnaires are not ready made. Researcher has to first develop research design and identify variables to be examined. Then questions are framed accordingly after sample size and sample universe is identified. hence proceed step by step.
From previous research models :
1. Advertisement Value (from Ducoffe’s Model, 1995)
A common base for YouTube ad research.
Subdimensions:
Informativeness
"The ad provides valuable information about the product."
"I find YouTube ads useful for learning about new products."
Entertainment
"YouTube ads are often entertaining."
"I enjoy watching some of the ads before videos."
Irritation (reverse-coded)
"YouTube ads interrupt my experience negatively."
"I find YouTube ads annoying."
Credibility
"I believe the information provided in YouTube ads."
"YouTube ads are trustworthy."
2. Purchase Intention
Adapted from studies like Pavlou (2003), Dodds et al. (1991)
"I am likely to purchase products I see in YouTube ads."
"I would consider buying a product because of a YouTube ad."
"YouTube advertisements influence my buying decision."
3. Attitude Toward the Ad (Optional but often included)
From MacKenzie & Lutz (1989)
"I like the YouTube ad I just saw."
"This ad is interesting and enjoyable."
4. Continuance Intention or Ad Engagement (Also optional)
Useful if you're exploring behavioral effects beyond purchasing.
Example Papers You Can Refer To:
"The Impact of YouTube Advertising on Purchase Intention: The Mediating Role of Advertising Value" – Usually uses SEM and similar scales.
Logan, Bright, and Gangadharbatla (2012) – Adapted Ducoffe’s model for social media advertising.
Mir (2012) – Credibility, informativeness, and entertainment in advertising.
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Only 1 question in survey - less than a minute
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Price and budget, resale value of that car, company reputation eg suzuki, Toyota etc,comfort and features, after sales service network, safety ratings etc
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Hello all, I am interested in purchasing a dox-inducible system that contains either GFP or RFP to transduce into cells and use for my animal studies. The cells will also contain luciferase as I will be detecting the growth of these cells in the animals using bioluminescence imaging. Will having GFP or RFP interfere with the bioluminescence signal and alter my results?
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Dear Brittany,
This literature provides a comparison between GFP luminescence and luciferase-luciferin imaging for your reference: PMID: 38925805.
GFP (Green Fluorescent Protein) has excitation/emission peaks at 397 nm and 509 nm, while RFP (Red Fluorescent Protein) exhibits peaks at 532 nm and 588 nm.
For luciferase-luciferin imaging experiments: The principle involves D-luciferin being catalyzed by luciferase to produce chemiluminescence at 560 nm. Importantly:
  • No excitation light within specific wavelength ranges is required
  • GFP and RFP will not generate fluorescent signals in this system、
Hope this helpful and feel free to reach out.
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Does anyone know where to purchase research-grade Galleria mellonella in Europe?
I only find the TruLarv from BioSystems Technology, but it seems to be down...
Thank you!
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Did you find a solution? We are looking to buy as well.
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I need to work in an arthritis model induce by serum from K/BxN. Do you know where can I purchase K/BxN serum?
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Hi! I am thinking about the same model. Did you find any solutions?
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For microfloatation with limited material availability
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Hi Evody, were you able to find a place to buy these glassware? I am also looking. Please let me know if you find out. Thanks
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Dear all,
I have just purchased an aliquot of lncap from atcc, I thawed them and the result was disastrous. Briefly, these cells form big clusters and even when they adhere they maintain this morphology which seems far from the one in literature. Atcc told me that the RPMI I used (which is the same I employ for other lines) is too low in glucose, it omits sodium pyruvate and has HEPEs and sodium bicarbonate different from the one in the suggested RPMI. My ignorance, I wasn't aware that differences between RPMI from different suppliers were so important. In addition they told me I made a mistake using heat inactivated FBS as, probably, I eliminated some growth factors.
Could you kindly tell me if, in your opinion, are these requirements all mandatory for a satisfactory culture...it is so crazy. Thank you in advance
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Simon Stuecheli They formed clumps. By the way, after several passages and patience they gained their conventional morphology
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I purchased the Novex Native PAGE gels, did Native-PAGE electrophoresis, and continued transferring the protein to the PVDF membrane by western blotting. But I can`t see my protein marker from NativeMark™ Unstained Protein Standard (LC0725).
I have added 4x sample buffer or G250 additive to the NativeMark™ Unstained Protein Standard, but the result still showed no protein marker band.
I would appreciate your advice if anyone has any experience with Western Blot following Novex Native Page. Thank you very much.
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I am having this problem too! Did you solve it?
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Unfortunately the Clay Mineral Society (clays.org) no longer have any pure illite in stock, and we need some for some experiments. If anyone knows where to find some, or has any of the old IMt-1 or IMt-2 CMS reference materials gathering dust in their lab, I'd really appreciate the input.
Thanks all,
Michael
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Unfortunately not, sorry Saheed.
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I purchased some alkali lignin a couple of days ago. From the literature study, I thought alkali lignin was not soluble in water. However, the alkali lignin I purchased is completely soluble in water, so I am confused about its solubility now.
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Dear Doctor
Go To
The depolymerization of lignin in water/acetone/formic acid synergistic solvents to produce high-value added phenolic monomers without external hydrogen and catalyst
Xinxu Zhao, Chaoqun You, Xun Li, Yu Zhang, Fei Wang
Elsevier
Fuel Processing Technology
Volume 261, 1 October 2024, 108102
[Highlights
•The chemical structures of solubilized lignin changes in water, acetone, and water/acetone medium.
•Water/Acetone mixed solvent displayed a good solubility (95.24%) for alkali lignin.
•High phenolic monomers yield of 57.48% and bio-oil yield of 81.45 wt% were obtained at 300 °C for 120 mins in water/acetone/formic acid medium.
Abstract
The limited solubility of lignin in commonly used solvents poses a challenge for its depolymerization into high-value monomers. This paper investigates the solubility of alkali lignin in water, methanol, ethanol, acetone, 1,4-dioxane, and their binary solution, and examines their impact on lignin depolymerization. The distribution of depolymerization products was correlated with the chemical structure changes in various solvent. Among the solvents tested, water-acetone mixtures demonstrated exceptional solubility for alkali lignin (95.24%) and provide the highest yield of bio-oil and phenolic monomers. The enhanced solubility of guaiacol units in acetone, combined with the addition of water in the co-solvent system dramatically improved the solubility of alkali lignin. Moreover, formic acid donated hydrogen protons to facilitate lignin depolymerization and prevented the repolymerization of unstable intermediates. Optimal reaction conditions were achieved at 300 °C for 120 mins using a mixed solvent composed of water, acetone, and formic acid in a ratio of 5:5:1 (v/v/v), corresponding to the highest yield of bio-oil with 81.45 wt%, the lowest yield of residue with 6.20 wt%, and a phenolic monomer content of 57.48%. Furthermore, this co-solvent system revealed satisfactory adaptability for converting various lignin into phenolic monomers.]
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Can everyone share their experience with the brand of micro-scissors, blade type (straight or curved), and affordability? Our lab only has one from F.T.S. 15004-8, and it's getting old. We repeatedly use it for mouse retina dissection, and we would like to purchase another one as a spare. I was wondering if there are more affordable brands out there that can do the same thing.
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According to my Brother a senior surgeon in USA, following is advise:
For lab work like mouse retina dissection, having precise and durable micro-scissors is crucial. F.T.S. 15004-8 is known for its high quality, but if you're looking for alternatives that are more affordable, there are several brands and factors to consider.
Popular Brands:
Dumont: Known for their precision, Dumont scissors are used in various fine dissection tasks. They offer both straight and curved blades, and while they are not the cheapest, their durability and sharpness make them a good investment in the long run.
Sable & Ziegler: Another high-quality brand that offers curved and straight micro-scissors. They are often a bit more affordable than Dumont but still maintain excellent performance for delicate dissections.
Eppendorf: Eppendorf’s micro-scissors are designed specifically for delicate biological dissections and are slightly more affordable. Their quality is often on par with the premium brands like Dumont but can be more accessible for lab budgets.
Vannas: Vannas are very popular in the field for micro-dissections, offering both straight and curved models. They're fairly affordable and long-lasting, often recommended for procedures like yours.
Mandel: Offers cost-effective options that perform well in tasks like retina dissection. They're a great alternative if you're looking for something more affordable without sacrificing too much precision.
Blade Type (Straight vs Curved):
Straight Blades: These are ideal for making clean, precise cuts where you need a flat edge. They're better for cutting through flat tissues or for more controlled dissection.
Curved Blades: Curved scissors allow for more delicate and precise cuts around tight areas or when working on circular or curved structures like retina. Many labs prefer curved blades for tasks like retina dissection because they offer better maneuverability in confined spaces.
Affordability:
While high-end brands like Dumont and Sable & Ziegler can be expensive, there are several affordable alternatives that can still meet your needs for mouse retina dissection. Brands like Eppendorf and Vannas often offer scissors that are priced competitively and still provide excellent quality and precision.
since you want only one as standby or replacement "Bulk Purchasing" options is ruled out.
Please check if suppliers offer discounted price for academic purposes.
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  • Define Your Financial Goals: Determine what you want to achieve (e.g., retirement savings, home purchase, vehicle purchase, wealth accumulation, education funding, and children's marriage) and the time-horizon for each goal.
  • Assess Your Risk Appetite: Evaluate your comfort with risk, considering your age, financial position, and emotional ability to handle market volatility.
  • Understand Your Investment Horizon: The period for which you plan to invest impacts your investment portfolio. Longer horizons often favour riskier investments (like stocks), while shorter ones may require safer options (like bonds or Fixed Deposits or Money Market Instruments, etc.).
  • Evaluate Your Current Financial Health: Take stock of your income, expenses, debts, and existing investments to understand how much you can allocate toward your portfolio.
  • Diversify Across Asset Classes: Include a mix of assets such as stocks, bonds, real estate, bank deposits, bullion, AIFs, and cash to spread risk and improve potential returns.
  • Consider Fees and Taxes: Factor like costs associated with investment (e.g., fund expense ratios, advisory fees) and tax implications to maximize net returns.
  • Monitor and Rebalance Regularly: Imperative to periodically review your portfolio to ensure it aligns with your goals and risk appetite. You can make adjustments as needed owing to market changes or life events.
  • Seek Professional Advice When Needed: If you're unsure about building or managing your portfolio, better consult a financial expert or use robo-advisors for customized recommendations.
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Constructing and maintaining the best investment portfolio requires a strategic approach that balances risk, return, and diversification while aligning with your financial goals and time horizon. Here’s a step-by-step guide to help you build and manage an effective investment portfolio:
1. Define Your Investment Goals
  • Clarify objectives: Understand why you're investing—whether it's for retirement, purchasing a home, funding education, or building wealth. The purpose of the investment will shape your strategy.
  • Time horizon: Determine how long you plan to hold the investments before you need to access the funds. The longer the time horizon, the more risk you can typically afford to take.
  • Risk tolerance: Assess how much risk you are willing and able to take. Risk tolerance depends on factors like age, financial situation, and comfort with volatility.
  • Income vs. growth: Are you looking for steady income (through dividends or interest) or long-term growth (through capital appreciation)? This will guide your asset allocation choices.
2. Asset Allocation
  • Diversify: Asset allocation refers to the way you distribute your investments across various asset classes (stocks, bonds, real estate, commodities, etc.). A well-diversified portfolio reduces the risk of large losses by spreading investments across different sectors, industries, and geographies.
  • Choose the right mix:Stocks: Typically offer higher returns over the long term but come with higher volatility. They are a good option for long-term growth. Bonds: Provide income and are generally less volatile than stocks, making them useful for stability and lower risk. Real Estate: Offers diversification and potential for income and capital appreciation. Commodities: Include investments in precious metals, energy, and agricultural products, offering protection against inflation. Cash or Cash Equivalents: Keep a portion in liquid assets (like money market funds) for flexibility and to manage short-term needs.
  • Consider your age and risk profile: Younger investors may prioritize stocks (more risk, more growth potential), while those nearing retirement might focus more on bonds and dividend-paying stocks (lower risk, income generation).
3. Select Individual Investments
  • Stocks: Choose stocks based on factors such as company fundamentals (growth prospects, financial health), industry trends, and valuation (price-to-earnings ratio, price-to-book ratio).
  • Bonds: Decide between government, municipal, and corporate bonds based on risk and yield. Government bonds tend to be safer but offer lower returns, while corporate bonds may provide higher yields but carry more risk.
  • Mutual Funds & ETFs: These pooled investment vehicles offer diversification within a single investment. They can focus on a specific sector, region, or asset class. Actively managed funds can offer higher returns but tend to have higher fees, while index funds and ETFs are passive and generally have lower fees.
  • Alternative Investments: Depending on your risk tolerance, you may consider private equity, venture capital, hedge funds, or cryptocurrencies, though these are generally riskier and less liquid.
4. Risk Management & Diversification
  • Diversify across asset classes: Ensure that your portfolio is not overly concentrated in one asset class (e.g., stocks or bonds) or one geographic area (e.g., just U.S. or emerging markets).
  • Consider sector diversification: Spread your stock investments across different industries such as technology, healthcare, consumer goods, energy, and financials. Each sector performs differently under various economic conditions.
  • International diversification: Including international assets (stocks or bonds) can help reduce risk associated with the domestic economy and increase growth opportunities.
  • Rebalancing: Over time, your portfolio’s asset allocation may shift due to market movements. Rebalancing ensures that your portfolio stays aligned with your risk tolerance and goals.
5. Maintain Liquidity
  • Keep a portion of your investments in liquid assets (like cash or short-term bonds) that can be easily accessed in case of emergencies or unexpected expenses.
  • The appropriate liquidity level depends on your specific financial situation and risk tolerance, but generally, 3-6 months of living expenses in cash or cash equivalents is recommended for an emergency fund.
6. Cost Management
  • Expense ratios: Pay attention to the expense ratios of mutual funds and ETFs. Lower costs can lead to better net returns over time.
  • Transaction fees: If you are actively buying and selling, consider transaction costs such as brokerage fees. Use low-cost or commission-free platforms if possible.
  • Tax efficiency: Optimize your portfolio to minimize taxes. Consider tax-advantaged accounts like IRAs or 401(k)s in the U.S., and choose investments with favorable tax treatment (e.g., long-term capital gains vs. short-term gains). Tax-efficient funds or ETFs can also reduce your tax burden.
7. Monitor and Adjust the Portfolio Regularly
  • Track performance: Regularly review the performance of your portfolio to ensure it meets your goals. Compare it to relevant benchmarks, like the S&P 500 for U.S. stocks or a bond index for fixed income investments.
  • Rebalance as needed: Rebalancing involves selling some investments and buying others to maintain your desired asset allocation. This should be done at least once a year or whenever there are major market fluctuations.
  • Adapt to changing circumstances: As your financial situation changes (e.g., you get married, have children, or near retirement), you may need to adjust your investment strategy, asset allocation, and goals.
8. Avoid Emotional Investing
  • Stick to the plan: It's easy to make emotional decisions during market volatility (buying during a rally, selling during a crash). Stay disciplined and avoid chasing short-term trends.
  • Focus on long-term goals: Remember that investing is a long-term strategy. Don’t panic in the face of short-term market fluctuations, and avoid making rash decisions based on emotions like fear or greed.
9. Seek Professional Advice
  • If you're unsure about building or maintaining your portfolio, consider working with a financial advisor or portfolio manager. They can help you develop a tailored investment strategy based on your unique financial situation, risk tolerance, and goals.
  • Robo-advisors are also an option for those seeking a low-cost, automated approach to investing. They use algorithms to help build and manage portfolios based on your goals and risk profile.
10. Long-Term Focus
  • Patience: Building wealth through investing takes time. Focus on the long-term growth potential of your portfolio and avoid trying to time the market.
  • Continuous Learning: The investment world is constantly evolving. Stay informed about new investment strategies, products, and market trends, but avoid getting swept up in the latest "hot" investment
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What are the purchasable ligand databases which are currently also updated.
I have been looking into Zn purchaseable db through Pharmit server, it is really not updated and the ligands are no longer buyable.
Other than Zn I can think of FDA-approved ones. But are there any other database other that these two, taht has a buyable section?
Thanks
Sanjukta
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What is OA?
I am into CNS databases.
Thanks but IMPAAT is not entirely buyable. I am looking for ones that are actually buyable.
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I am currently conducting my undergraduate thesis and I need MRS agar and broth, however, the one accessible to me (that I am familiar with) is from HiMedia, which only comes in 500 g bottles (I only need like 100 g of).
Has anybody tried purchasing culture media from BaseBio (?) or Phygene (?)? I have never heard of these brands before which is why I am hesitant to purchase but they are the only ones that come in 100 g bottles.
Let me know how it went. Thank you!
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BaseBio has been gaining traction as a reliable supplier of microbiological and laboratory-grade culture media. They are commonly recognized for their competitive pricing and flexible packaging sizes.
Availability of smaller quantities like 100 gm bottles, which can reduce waste and cost.
Reports suggest their products meet the quality standards for routine lab use.
Known for consistent performance with commonly used media like MRS agar and broth.
It is essential to confirm the certification and validation standards for your specific experiment. Ensure they adhere to ISO or GMP manufacturing standards if necessar
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We need to replace our large muffle furnace and the replacements are extremely expensive for the size we need. Can I purchase a high-end, vented kiln instead? Is there a difference in the two pieces of equipment that make them not interchangeable?
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Yes, you can consider purchasing a high-end, vented kiln as an alternative to a large muffle furnace, but they are not entirely interchangeable due to some key differences in their design, functionality, and intended use. Hence they are interchangeable subject to comparison below. If that falls in your working parameters range. Go ahead
Here are the key differences between a muffle furnace and a vented kiln:
Maximum Operating Temperature: A muffle furnace can reach up to 1,600°C or higher, while a vented kiln typically operates up to 1,400°C or lower.
Heating Environment: A muffle furnace uses a fully sealed environment, ensuring uniform and controlled heating. In contrast, a vented kiln allows gas exchange through its ventilation system.
Primary Use: Muffle furnaces are commonly used for sintering, material testing, metallurgy, and calcination. Vented kilns are primarily used for ceramic production, pottery, and glass firing.
Temperature Precision: Muffle furnaces offer very high temperature precision, while vented kilns have moderate precision.
Ventilation: Muffle furnaces lack ventilation to maintain complete isolation, while vented kilns include ventilation mechanisms to allow gases or byproducts to escape during firing.
Typical Applications: Muffle furnaces are suited for laboratory testing and sintering metals, while vented kilns are best for ceramics, pottery, and glass-related firing processes.
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Hi all,
I am looking for type material of fossil terrestrial gastropods described by August Reuss. Specifically I want to find the syntypes of Vertigo callosa Reuss, 1849 from the Early Miocene of Tuchorice (CZ). Reuss worked, amongst others, in Prague and Vienna, and parts of his collections (microfossils) were purchased in 1891 by the Natural History Museum in Vienna. However, the mollusks aren't there, and I couldn't find any information on their whereabouts, apart from a few author's statements that the collection "might be lost". It remains uncertain though if anyone actually made a serious effort to locate it.
Any hint or suggestions would be most welcome!
Best wishes,
Thomas
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Dear Marc,
Thanks for the tip! I contacted them, hopefully something will turn up.
Best wishes,
Thomas
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How the backing plate can effect sputtering during process?
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Phil Denby Why is RF sputtering NOT recommended for ferromagnetic metallic targets? Could you please elaborate a little bit? Is it due to eddy current formation? Could you please share any reference articles?
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The cell line I plan to purchase from ATCC is not readily available. So the supplier suggested to try from iCell Bioscience, China. I have not heard of this cell supplier, so trying to see feedbacks and review from others.
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Sorry sir... I didnt have experience.
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I’m particularly interested in AI-driven simulation tools that can predict or model consumer behavior, focusing on areas such as purchasing decisions, market segmentation, or consumer preferences. If anyone has recommendations or has worked with such tools (besides the Consumer Behavior Spectrum Model), I would love to hear your insights!
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Rapid Miner software provides many statistical methods, allowing you to analyze market basket by applying association rules mining, customer segmentation based on clustering algorithms....
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Would it be possible to think that if there is a categorization regarding fundamental rights, the state should, in the same way, prioritize the budget, ensuring that the rights higher in the prioritization receive more resources? That is, why allow the state to allocate more resources to a policy that has to do with the disappearance of some autonomous organizations, regarding policies, infrastructure, projects that help minimize water waste, support the purchase of medicines? against cancer, among others. Wouldn't it be desirable to modify the way resources are allocated today?
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totally agree. Barriers and paradigms must be broken in the definition of budgets. One of the first obstacles is how to define those fundamental values. From my perspective, life should come first, but there are positions that disagree. A lot to think about and develop, thank you very much for your response, I really appreciate it.
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The future of nuclear power depends upon several factors. One of these factors is the use of small module reactor (SMR) for electricity generation and for other non-electric applications. Several prototypes of SMR are under development in several countries, but only four of them at this moment are under construction in three countries. This type of nuclear power reactor will receive the approval of the public opinion of those countries thinking to expand or to introduce nuclear energy for electricity generation in the coming years and will allow the construction of this type of reactor in their countries? Which are these countries?
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I think many states will be adopt SMR option; USR, UAE,.....
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Use the Temu coupon code 'acs540599' to enjoy an incredible 90% discount on your next purchase. Plus, every order includes free shipping and hassle-free returns!
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See the above comment. Do not litter this platform with this garbage.
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Grab an unbeatable 90% discount on your next Temu purchase using the code 'acs540599.' Free shipping and returns are included with every order!
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Do not transform RG from a platform for professionals into another commercial marketplace. Your post must not be here!!
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Hi all.
My lab is starting chemotherapy drug sensitivity assays for oral cancer cell lines. We will be detecting cell viability with the MTT and SRB assays, both of which are fluorometric. Hence, we'll need to purchase a fluorescence microplate reader.
Which brand's plate reader/product is the best? We are looking into affordable options that work for 96 well plates and ideally other formats as well. Is there one you guys are using in your lab that is good and efficient?
It would be really helpful if I could be guided into making the right purchase!
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there are many good provider of fluorescence readers
eg. Tecan, Molecular device,
from my point of view the essential is that the instrument is not equiped only with filters but also with a monocromator, which allow you to free select any excitation/emission wavelenght that you mayneed in the future.
best regards
Manuele
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I need to purchase paramagnetic magnetic nanoparticles ~500nm for magnetic manipulation and magnetic swarm formation.
Are there any companies that sell the product with international delivery (to South Korea).
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Clemente Associates has these products.Clemente Associates LLC
1947 Atlantic Ave
Prescott, AZ 86301
ph.928 227 0893 clemasso@gmail.com
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I am planning to use prenyl binding protein PDED inhibitor Deltazinone 1 but I am unable to find any supplier?
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Hello, Shweta
You could buy them from TargetMol which specializes in products and services that serve the research needs of chemical and biological scientists worldwide.
Here is the product link.
If you have any questions, please do not hesitate to contact inquiry@targetmol.com or visit the website www.targetmol.com.
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Im working on fungal taxonomy,
I would like purchase suitable camera.
I welcome you guys to suggest best.
regards,
Niranjan
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A lot of people like the Olympus Tough TG-6 or TG-7. It’s very tough, small, takes good micro pictures and is able to photo stack which means every part of the image is in focus. It’s particularly good with smaller fungi
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We wish to incubate and shake the 96 well plate, and measure the OD reading of bacteria.
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For installing a compact plate reader inside an anaerobic chamber with incubation and shaking capabilities for 96-well plates, I have a few suggestions to consider:
  1. Size and compatibility: Ensure the plate reader is compact enough to fit inside your anaerobic chamber and is compatible with anaerobic conditions.
  2. Incubation and shaking features: Look for a model that offers both temperature control and shaking capabilities.
  3. Gas-tight design: The reader should have a gas-tight design to maintain anaerobic conditions.
  4. Remote operation: Consider a model with remote operation capabilities to minimize chamber openings.
  5. Connectivity: Ensure the reader can transmit data outside the chamber easily.
  6. Power supply: Check if it can operate on battery or if it needs a sealed cable feed-through.
  7. Maintenance: Consider ease of maintenance in anaerobic conditions.
Some potential options to explore include:
  • BioTek's Cytation 1 Cell Imaging Multi-Mode Reader
  • Molecular Devices' SpectraMax ABS Plus Microplate Reader
  • BMG LABTECH's CLARIOstar Plus
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The literature on Supply Chain Integration (SCI), Supply Chain Management (SCM), and collaborations, spanning several decades, deserves special attention. This topic, although addressed for a long time, remains contemporary and continues to be the subject of in-depth studies. The interest lies in understanding why collaboration in the supply chain remains a highly studied research topic, particularly concerning supplier relationships.
To achieve this, this study is organized around SCM and SCI, then purchases intended for sale in general, followed by strategic purchases, and finally the purchasing function in SMEs and its contribution to performance. The aim is to understand the purchasing behavior and competencies of SMEs, focusing on practices and examining their impact on value creation and long-term competitive advantage.
For this reason, this study aims to analyze supply chain management in a disrupted context. The objective is to discover how to optimize the purchasing function within an Extended Enterprise. This optimization seeks to identify actions to undertake to enhance its competitiveness and help it address the difficulties it faces, particularly against powerful suppliers who hold negotiating power, while considering its limited resources and competencies.
What is your perspective on this topic, both in terms of its relevance and the scientific validity of the potential results, and why?
Thank you
Nicolas
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Collaborating with key suppliers significantly enhances an SME's competitiveness by providing access to vital resources, cost savings, and increased innovation.
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After lapse of a few months, we recently attempted insulin-tolerance tests in C57BL/6N female mice at 6 months of age. We began, years ago, with Humulin-r at 0.75 U/kg. In our recent tests we used the same protocol, but the only response was a slight increase in blood glucose! We tried several higher doses. At 1.75 U/kg a very slight decrease was observed, but not until 60 minutes. We purchased a non-clinical preparation of insulin from a company that sells a lot of growth factors and cytokines. It produced the puzzling increase in blood glucose at both 0.75 and 1.25 U/kg. Has anyone else had a problem with insulin efficacy of late? I can't believe all our mice are suddenly insulin resistant!
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Bevacizumab and ranibizumab are human-derived anti-VEGF antibodies which doesnot have any effect on rodent retinal or choroidal neovascularization. Humulin-r is a human-derived insulin. With a possibly similar reason, it doesnot work on rodents.
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I have the Holtzman Inkblot Test Cards Form A, which I was able to purchase from an individual who had a set from 1958. I am now looking for Form B. I wish to do some research with this set of cards and cannot find them anywhere. If anyone has a set Form B I am willing to purchase them. My email is jspores@pnw.edu. Thank you.
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Yes, I would be interested in doing research with the Holtzman technique. Presently, I use the Rorschach Performance Assessment System® (R-PAS®), but was interested in determining if the Holtzman cards will provide superior reliability and validity. I am still search for Form B because I want to determine reliability of Forms A and B. Would you know anyone who has Form B?
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In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and αSMA was very high, almost comparable to the TGF-β group. The culture medium for HK-2 cells is DMEM/F12 + 10% FBS + 1% PS. I am uncertain if I made any mistake that caused the HK-2 cells to start fibrosis on their own. If anyone could provide some advice, I would greatly appreciate it. Thank you!
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The cell culture medium formulation and its modification can affect the HK-2 cell behavior during TGF-β1 induced in in vitro EMT. The HK-2 cell line is a proximal tubule cell line, and proximal tubule is the major player in glucose reabsorption. So, HK-2 and other proximal tubule cell lines are used for studying mechanisms of diabetic kidney disease which is the foremost cause of renal failure.
You may want to refer to the article attached below for more information.
Your media for routine culture would be DMEM/F12 (high glucose). But for treatment media, you may use DMEM/F12 (low glucose).
The study in the paper attached below suggest that the levels of vimentin and α-SMA increased in the high glucose group compared with the control group. More information may be obtained from the article.
Some more articles for your reference.
Best.
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Does anyone know if there is a lab scale encapsulation machine available for purchase?
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Buchi has a lab scale encapsulator
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Penconazole
1. CGA 132465
2. CGA 190503
3. CGA 127841
Fluopyram
1. AE C656948-benzamide
2. AE C656948-pyridyl-acetic acid
3. AE C656948-carboxylic acid
I have searched on sigma aldrich as well as on dr. erhenstofer site but i did not find any results.
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Thank you sir Chen Jingwen
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We are normally using wet transfer for our mini gels for western. We would like to to purchase a new set-up for big gels (20x20cm), with this regard we would like to know which transfer system is better semi-dry or wet? Which would give us a more reliable result ? Time and money is not in question but the main concern would be the final outcome.
Thanks in advance for your help.
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In general, wet transfer tends to provide better results than semi-dry transfer for protein transfer in Western blotting, offering higher efficiency and more consistent transfer across a wider range of molecular weights.
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article
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You might find interesting the reading of these documents:
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For example, when selling the service products, female seller has greater influence than male seller on customers' purchase intention. However, I want to find out when selling a normal product, female seller's strength was lower but not necessary be lower than male seller. I was wondering whether there is a technique can help me to find out the "female seller's strength was reduced when selling the normal products when comparing to her own performance when selling the service product, but was always better than male sellers"? In other words, I want to find out "female seller's influence is always stronger than male sellers, but when selling different types of products, female seller's overwhelming strength would be fluctuate". In this model, the moderator is the two types of products (service and normal product), which are coded as 0/1 variable. The antecedent is the two different sellers (female and male seller), which are coded as 0 or 1 variable.
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The customer expectations are rising. They want to eliminate the complexity involved in solving their problem and they want - we need to know when it's appropriate to balance automation vs. human outreach. If we fail to acknowledge this shift, we can expect our brand perception to take a hit. But if we get the wheels turning back in the right direction and placing an emphasis on the importance of the perceived experiences.
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I recently acquired a used Illumina HiSeq 2500 and am in need of flow cells compatible with this machine. As I am relatively new to this aspect, I would greatly appreciate any recommendations on reliable suppliers or vendors where I can purchase these flow cells. Thank you in advance for your assistance.
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I think it is no longer supported - 'The HiSeq 2500 System has been discontinued. Illumina will support the instrument and intends to supply the reagents through February 28, 2023. To find an alternative instrument, use the Sequencing Platform Comparison Tool.' https://support.illumina.com/sequencing/sequencing_instruments/hiseq_2500.html
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HUMOROUS MORPHOLOGY
In Lewis Carroll’s Alice’s Adventures in Wonderland, the Gryphon used the word “uglification,” and Alice asked the Gryphon what the word meant. The Gryphon was amazed that Alice didn’t know this word. He asked Alice if she knew the meaning of “beautification,” and Alice responded that she did, but she still didn’t think that “uglification” was a real word with a real meaning. Indignantly, The Gryphon said that a word means whatever you want it to mean, and then he went on to give other examples. He said that what is taught in the schools is “reeling,” “writhing,” “uglification,” and “derision.”
Teachers should be called “tortoise” because “they taught us.” Lessons are called “lessons” because they lessen every day. In Wonderland, “Latin and Greek” are called “Laughing and Grief,” and “drawing, sketching and pointing in oils” becomes “drawling, stretching, and fainting in coils.”
The Watergate Hotel is where the break-in of the National Democratic headquarters occurred. Today’s dictionaries give more room to the metonymous meaning of Watergate than to the literal meaning of “a gate controlling the flow of water.” “Gate” has now become a suffix meaning “scandal” as in Irangate, Contragate, Iraqgate, Pearlygate, Rubbergate, Murphygate, Gennifergate, Nannygate, Monicagate, ad infinitum.
On National Public Radio’s “Cartalk,” Click and Clack are playing with Morphology in their list of credits: Copyeditor: Adeline Moore, Accounts Payable: Ineeda Czech, Pollution Control: Maury Missions, Purchasing: Lois Bidder, Statistician: Marge Innovera, Russian Chauffeur: Picov Andropov, Legal Firm: Dewey, Cheetham, and Howe.
Another kind of humorous morphology is when words are translated (actually transliterated) from one language to another. “Un petit d’un petit / S’étonne aux Halles” makes no sense in French, but it makes perfect humorous sense in English: “Humpty Dumpty / Sat on a wall.”
In summary, the inflectional and derivational morphology of English Nouns, Verbs, Adjectives, Adverbs, and Pronouns have an amazing capacity to morph into other Nouns, Verbs, Adjectives, Adverbs, and Pronouns.
Can you think of other humorous examples of English words morphing into other words?
Don and Alleen Nilsen “Humor Across the Academic Disciplines” PowerPoints:
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You can use stories and comics to demonstrate how morphemes can create ambiguity, irony, humor, or emphasis. You can also use stories and comics to explore how morphology varies across languages, dialects, registers, and genres. I found a few examples. Spell Analysis: Break down spell names into roots and affixes. For example, "Lumos" (light) to explore word formation.
Character Name Etymology: Study the morphology of names, such as "Lupin" from "lupus" (wolf), to gain insight into characters.
Spell Making: Have students create spells using Latin or Greek roots, practicing combining morphemes.
Potions and Ingredients: Use potion ingredients to discuss compound words and their components.
Magical Creature Classification: Classify creatures based on the morphemes that indicate their traits.
Wizarding Slang: Analyze slang (for example, "Muggle") to gain insight into word formation. I hope this helps.
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I am unable to find them on common cell banks like ATCC. Thank you!
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The Cellosaurus entry for Hey A8 does not contain any link to a trusted organization distributing that cell line:
So as we describe in one of our FAQ:
"If you are unable to find and organisation that distributes your cell line of interest, you could try to contact the laboratory that established it or, if it is no longer in activity, a laboratory that makes use of that cell line."
Here is a paper from a lab in Texas, so not far from you, that has used Hey A8 recently:
Good luck
Amos Bairoch
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According to my latest co-authored research with Dr Dharmendra Naidu that is published in a prestigious journal, “The Accounting Review”, TAR:
“We find that customers’ financial reporting quality is positively associated with future supply contracts, indicating that suppliers are more willing to contract with customers who provide them with better information”, (Naidu and Ranjeeni, 2024).
Pleased to share that our research is available for free downloading and reading for a limited time. Please access our published paper in TAR using the DOI below:
Abstract
Using a unique hand-collected dataset of purchase obligations, we find that customers’ financial reporting quality is positively associated with future supply contracts, indicating that suppliers are more willing to contract with customers who provide them with better information. Further, the association between customers’ financial reporting quality and future supply contracts is stronger for customers with strong bargaining power, which is consistent with suppliers relying more on financial reports when they have less access to customers’ private information. Collectively, our results suggest that customers’ financial reporting quality plays an important role in influencing suppliers’ decisions to contract with customers for the future supply of goods and services.
Reference:
Dharmendra Naidu, Kumari Ranjeeni; Is Customers’ Financial Reporting Quality Associated with Suppliers’ Decision to Contract?. The Accounting Review 2024; https://doi.org/10.2308/TAR-2021-0652
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Thank you!!! Congratulations Kumari!
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The fluorescent labelled primer for the expected sample is purchased 2 years ago and for the sample with intense signals is purchased 3.5 years ago and have been kept in the identical condition ever since.
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The large signal from very small dna is to be expected since the sample loading into the capillary is electrokinetic so loading is more efficient with small (very mobile) and charged molecules so the sequencer loads small highly charged fragments preferentially. If you loaded the plate again you would expect better results because you have desalted the input sample to some extent during the first loading.
The answer may be to re analyse the plate results starting a few minutes later so as to exclude the huge salt peaks (primer peaks). Speak to whoever runs your samples /tech help to discuss re analysis.
What I meant by my earlier answer was that to avoid too many peaks being analysed which are just background noise there is a fixed percentage cut off defined by early peak height under which peaks will not be analysed. As the ladder is often quite a weak signal when there is a large early peak the software may ignore the ladder peaks making the sizing part of the analysis impossible since some or all ladder peaks are being ignored. I used to run my samples at 2 dilutions to try to get round this problem. Again re analysis with inclusion of more peaks by tweaking the analysis parameters should help
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This concise research question focuses on two main aspects:
  1. Impact of Augmented Reality (AR) on Purchasing Decisions: The question investigates how the use of AR technologies in online luxury fashion retail platforms affects the buying behavior of consumers in the UK. AR can offer immersive experiences where consumers can virtually try on clothing or accessories, enhancing their understanding of the product and potentially influencing their decision to purchase. This part of the question aims to understand whether AR features lead to more informed purchasing decisions, increase buyer confidence, or possibly even lead to higher sales conversions.
  2. Optimization Through Engineering Management Principles: The second part of the question explores how principles of engineering management can be applied to optimize the integration of AR in online retail settings. This involves looking at the systematic planning, design, and implementation of AR technologies to enhance operational efficiency, improve user experience, and ensure the sustainability of the technology. It considers how engineering management can address challenges such as technological integration, scalability, cost efficiency, and the alignment of AR technology with broader business goals.
The research will likely involve analyzing consumer behavior through data collection methods like surveys or interviews, and examining case studies or existing implementations of AR in luxury retail. It will also include an evaluation of how engineering management principles can be applied to streamline processes, manage technological changes, and enhance the overall effectiveness of AR technologies in engaging consumers and driving sales.
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The integration of Augmented Reality (AR) in online luxury retail has a significant impact on UK consumer purchases by enhancing the shopping experience, increasing confidence in product selection, and reducing the likelihood of returns. AR allows consumers to virtually try on products, visualize how items will look in their physical space, and explore intricate details, such as fabric texture and craftsmanship, all from the comfort of their own home. This immersive experience bridges the gap between the online and offline shopping experience, thereby fostering a deeper connection with the product and brand.
Engineering management can optimize AR integration in online luxury retail by prioritizing several key strategies:
User Experience Design: Invest in intuitive and seamless AR interfaces that enhance the overall shopping experience. This involves collaborating closely with UX/UI designers to create interfaces that are easy to navigate and provide realistic representations of luxury products.
Technological Innovation: Stay at the forefront of AR technology advancements to continually improve the quality and realism of virtual try-on experiences. This may involve leveraging computer vision, machine learning, and 3D modeling techniques to create lifelike renderings of products.
Performance Optimization: Ensure that AR features are optimized for performance across various devices and internet connections. This requires rigorous testing and optimization to minimize latency and ensure smooth rendering, particularly on mobile devices, which are increasingly becoming the primary platform for online shopping.
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The link for splicer description https://shinho.eu/s12pm/
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Panda Prof splicing
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After the Covid-19 pandemic, what do you think are the most serious potential sources of another economic and/or financial crisis that could occur in the future?
In recent years, the scale of the occurrence of serious economic and financial crises in some regions of the world and/or on a global scale has been intensifying. The scale of the appearance of certain types of economic, financial, energy, etc. crises has been increasing since the 1970s. The abolition of gold parity for the U.S. dollar, oil crises, deregulation and liberalization of the functioning of financial markets, increasing the active role of central banks in shaping monetary policies with the possibility of adding money injected into the economy through direct purchase of treasury bonds by the central bank, abolishing some of the previously introduced systemic prudential instruments used in credit risk management, increasing the scale of international operations of investment banks and investment funds making speculative transactions in foreign capital markets are just some of the sources of the increase in the scale and frequency of financial and economic crises since the 1970s. Some types of the aforementioned crises have appeared with increasing frequency and magnitude in the current 21st century. The day on September 15, 2008, when the world's fourth-largest investment bank Lehman Brothers went bankrupt was considered the beginning of the global financial crisis. This crisis was generated by, among other things, erroneously conducted overly lax monetary policies that were supposed to favor financial markets even when assets were overvalued in capital markets, overly liberalized credit policies in mortgage lending, disregarding safety standards in credit risk management and practicing moral gambling in investment banking, and so on. In 2020, there was a pandemic economic crisis, which initially developed through the Covid-19 pandemic to then be exacerbated by large-scale lockdowns imposed in some countries on economic entities operating in selected, certain industries, mainly service sectors of the economy, and the introduction of so-called national quarantines. In some countries, where, such as Poland, the development of the cheapest renewable energy sources was blocked and slowed down in 2022, when the price of fossil fuels rose strongly, a deep energy crisis occurred. Beginning in 2021, inflation began to rise rapidly in many countries, generated by pumping large amounts of printed money into the economies, whose task was to mitigate the scale of the recession generated by the lockdowns and national quarantines introduced repeatedly during the Covid-19 pandemic. In order to limit the growth of inflation, which, as in Poland, rose to double-digit levels, the central bank raised interest rates. The effect of such anti-inflationary measures was to reduce liquidity in the financial sector, increase the cost of borrowed money, make credit more expensive and reduce the scale of investment in many sectors of the economy. The result was a recession of the economy, which in many countries appeared in the 1st half of 2023. In view of the above, the misapplied measures of monetary and/or fiscal policy eased too much led to the generation of a financial and/or economic crisis. Subsequently, the anti-crisis instruments applied more than once led to the generation of another economic crisis. In addition, a climate and environmental crisis is also developing in the long term as a result of continued high greenhouse gas emissions, ignoring issues of protecting the planet's climate, biosphere and biodiversity, slowing down the development of renewable energy sources and implementing the green transformation plan for the economy on a limited scale. In view of the above, some crises like the pandemic economic crisis of 2020, among others, were generated by new factors like the Covid-19 pandemic, which could later be referred to as so-called “black swans” due to their uniqueness, atypicality and unexpected appearance by no one. On the other hand, the key root factors of some economic and financial crises include misguided state interventionism, errors in forecasting and analysis of the macroeconomic situation, misapplied pro-growth and/or anti-crisis instruments within the framework of certain economic, fiscal, budgetary, sectoral and monetary policies pursued by the government and conducted by the central bank. In view of the above, it is probably not possible to conduct economic, monetary, etc. policies without making mistakes. It is not possible to forecast all, future impact factors, determinants shaping the macroeconomic situation and potentially all events that may lead to further economic and/or financial crises in the future. However, it is possible to learn from past mistakes, and given this knowledge, a more sustainable, secure economy can be built, processes and instruments for managing credit risk and other categories of risk can be continuously improved, financial markets, including capital markets, commodity markets, securities markets can be systemically strengthened through prudent use of prudential instruments, not ignoring the principles of financial security, not practicing moral gambling in investment banking, and so on. Perhaps in the future, the next financial and economic crises that will occur will be the result of, on the one hand, still not adequately refined systemic prudential instruments, institutional financial security arrangements, credit risk management instruments, etc., and new factors and events that are difficult to forecast, which can probably later be called the next black swans. however, there are crises that we know will worsen in the future and/or will be the source of the occurrence of increasingly serious negative effects on the economy and humans. this kind of long-term crisis already in operation is the ever-developing and worsening climate crisis and, at the same time, the environmental crisis, which is associated with the process of rapid loss of biodiversity of the planet's natural ecosystems.
The key issues of the impact of the Covid-19 pandemic on the economy and financial markets are described in my article below:
IMPACT OF THE CORONAVIRUS PANDEMIC (COVID-19) ON FINANCIAL MARKETS AND THE ECONOMY
IMPACT OF THE SARS-COV-2 CORONAVIRUS PANDEMIC (COVID-19) ON GLOBALIZATION PROCESSES
The key issues of the problematic sources of Poland's exceptionally deep energy cross in 2022 are described in my co-authored article below:
POLAND'S 2022 ENERGY CRISIS AS A RESULT OF THE WAR IN UKRAINE AND YEARS OF NEGLECT TO CARRY OUT A GREEN TRANSFORMATION OF THE ENERGY SECTOR
Zarzadzanie kryzysowe w przedsiebiorstwie opisałem w artykule:
CRISES IN THE ENVIRONMENT OF BUSINESS ENTITIES AND CRISIS MANAGEMENT
I described the key issues of opportunities and threats to the development of artificial intelligence technology in my article below:
Anti-crisis state intervention and created in media images of global financial crisis
In view of the above, I address the following question to the esteemed community of scientists and researchers:
After the Covid-19 pandemic, what do you think are the most serious potential sources of another economic and/or financial crisis that could occur in the future?
What do you think are the most serious potential sources of another economic and/or financial crisis that could occur in the future?
What do you think about this topic?
What is your opinion on this issue?
Please answer,
I invite everyone to join the discussion,
Thank you very much,
Best wishes,
Dariusz Prokopowicz
The above text is entirely my own work written by me on the basis of my research.
In writing this text, I did not use other sources or automatic text generation systems.
Copyright by Dariusz Prokopowicz
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Several potential sources of another economic or financial crisis post-Covid-19 pandemic are worth considering:
1. Debt Overhang: Many countries, corporations, and individuals have accumulated significant debt during the pandemic to weather the economic downturn. If this debt becomes unsustainable or is not managed properly, it could lead to defaults and financial instability.
2. Asset Bubbles: Ultra-low interest rates and massive liquidity injections by central banks have fueled asset price inflation in various markets, such as stocks, real estate, and cryptocurrencies. If these prices detach from underlying fundamentals and then collapse, it could trigger a financial crisis.
3. Geopolitical Tensions: Escalating geopolitical tensions, trade conflicts, or even the outbreak of wars could disrupt global supply chains, dampen investor confidence, and lead to economic downturns.
4. Climate Change: The increasing frequency and severity of climate-related events pose significant risks to economies and financial markets. These include physical risks (such as extreme weather events damaging infrastructure) and transition risks (such as policies aimed at mitigating climate change impacting certain industries).
5. Technological Disruptions: Rapid advancements in technology, such as automation, artificial intelligence, and blockchain, could lead to job displacement, exacerbate income inequality, and disrupt entire industries, potentially causing economic dislocation.
6. Health Emergencies: While the Covid-19 pandemic has been unprecedented in scale, future pandemics or health crises could also wreak havoc on economies, disrupting supply chains, reducing consumer demand, and straining healthcare systems.
7. Cybersecurity Threats: With increasing reliance on digital infrastructure, cyberattacks pose a significant threat to financial systems, disrupting transactions, eroding trust, and causing financial losses.
8. Demographic Challenges: Aging populations in many countries, coupled with declining birth rates, could strain pension systems, reduce labor force participation, and slow economic growth.
9. Political Instability: Political unrest, polarization, or unexpected political events (such as coups or mass protests) can create uncertainty, discourage investment, and destabilize economies.
10. Natural Disasters: Beyond climate change-related events, earthquakes, tsunamis, hurricanes, and other natural disasters can cause widespread destruction, disrupting economic activity and leading to financial losses.
Addressing these potential sources of crisis requires proactive measures such as prudent fiscal and monetary policies, effective regulation and supervision of financial markets, investment in resilience and adaptation to climate change, and fostering international cooperation to address global challenges.
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I am looking into purchasing an automated cell counter. I have used the Countess previously, but was wondering if there are other recommendations for counters that are reliable? Are there any good cell counters that do not use disposable slides?
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Ruth Huttenhain Have you considered the LUNA-FX7 ( https://logosbio.com/luna-fx7/ )? it's 21 CFR Part 11/GMP compliant, has dual fluorescent and brightfield detection, and multiple chamber formats(8- , 3-, 2-, 1-, if I'm not mistaken). It ticks a lot of boxes.
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I'm gonna to make arthritis model induce by serum from K/BxN. However, K/BxN mice are unable to breed. So where can I purchase K/BxN serum?
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Hi Wang and Camila, could you solve the problem??
I would like to buy K/BxN serum but where can I do?
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I am planning to work on different water bodies of Andhra Pradesh. So I want to purchase a portable water testing kit with multiple parameters. Please suggest me a few best option, which would be a great help for me.
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Tintometer Lovibond photometer would be a one of good option
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I am looking for a compound called Tryptoquivaline F/J (or Fumitremorgin F/J) for some validation assays. Either to purchase or synthesize. Thanks!
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Are you still looking for tryptoquivaline F/J (or fumitremorgin F/J)? Please contact me, if you are still interested in these.
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For my reseach, I had purchased several frozen vials of HFSC(Hair follicle stem cell) from Celprogen, but I could not characteriz the Celprogen-HFSC with several anti-human antibodies for recognizing human HFSC. In addition, their mophology look like epithelial cell with round shape, and was growing very very fast like cancer cells. I did whole RNA sequencing of Celprogen-HFSC, and the data for Celprogen-HFSC indicated the cells were not definatly from human sources, perhaps was CHO cell. I claimed the Celprogen several times but they always answered there was no problem in managing cells in Celprogen. At that time, I did not want to waste my time to conflict with Celprogen any more. However, recently, one of my companion purchased a frozen vial of PDLSC (Periodontal ligament Stem cell) from Celprogen, but also he had the same experience as I had. The cell morphology is not mesenchymal lineage, showed epithelial like appearance with bursting proliferation activity. Hence, I asked anyone who experience such things after purchased from CelProgen. If yes, please answer here or send me email: yshwang@khu.ac.kr.
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We purchased what was advertised as "human portal fibroblasts" from Celprogen 2 yrs ago. I did not think of authenticating them because another investigator in our unit had used them in the past and had no apparent problem with them. I used them for a very complex and expensive study that included single cell RNA seq analysis among others. When the results came back, we found out the cells were neither fibroblasts nor human, and I confirmed that by RT-PCR, too. I contacted customer service on the phone and was told to send a detailed email with all the info (date of purchase, lot number, etc.). I never heard back from them. I gave up after the third email. As said above, Mayo Clinic has now put the company on a do-not-purchase-from list. I wish this was done 3 yrs ago - it would have saved us $42,000 and a lot of time! This company should go out of business.
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I am a postgraduate research student working on cancer biology in India. I need Danio rerio (Zebrafish) for this study of mine at the earliest. Can someone please provide me with contacts for places where I can purchase zebrafish in India and expect a quick delivery? I appreciate any help. Thank you so much for your attention and participation.
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You may contact Dr. Chetana Sachidanandan at chetana@igib.in
Phone: +91-11-29879-105
CSIR-Institute of Genomics & Integrative Biology(CSIR-IGIB)
CSIR-IGIB South Campus, Mathura Road, Near Sukhdev Vihar, New Delhi 110025.
Chetana Sachidanandan's lab uses zebrafish models to study human diseases and to screen for new drug-like molecules.
Besides the above, TIFR at Mumbai may also be able to help you.
The links below will be helpful.
Best.
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Please provide information on where I can purchase a 150 kDa, 500 kDa, 1000 KDa and 5000 KDa molecular weight cutoff membrane? for separation of ferulic acid (194 mwt) form mixtue of sample. where almost other molecular wt cut of is ranginng form 100 - 150.
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ok! I will check thank you for your time.
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I have strated lateral flow assay and the components are from clonegene. results are faint not clear...sometimes test line is coming sometimes control line is coming ..what should be the reason? Is there any paper availavle from LFA who have used clonegene components for my reference?
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Try drying the nc for longer. Are you using the proppike thater antigen conjugate for this?
The conjugate looks a bit dark, i would re check the conjugation conditions. The line should not look l
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There are two interference peaks in 1X PBS by DPV methods in three-electrode system which locate in 0.274V and 0.517v.
PBS was purchased from J&K Scientific Ltd which was blowed by nitrogen for 3 minutes before test in order to remove oxygen . And my working electrode was gold electrode made by sputtering. Ag/AgCl electrode was used as reference electrode.
Is there anyone could help me explain this phenomenon?
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Have you gotten some explains about this phenomenon? Recently, the silimar problem occured in my experiments. My working electrode was gold electrode CHI101, and Ag/AgCl electrode was reference electrode.One interference peak locked at 0.38v, in 10 mM tris (pH 8.0) by SWV methods .
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Dear Forum Members,
I hope this message finds you well. I am currently considering the purchase of a Q-TOF LCMS-9030 Mass Spectrometer for my laboratory, and I would greatly appreciate hearing from anyone who has experience working with this instrument.
If you have used the Q-TOF LCMS-9030, I would be interested in hearing your thoughts and experiences. Specifically, I am seeking reviews on the instrument's performance, reliability, user-friendliness, and any limitations or challenges you may have encountered.
Your insights will be incredibly valuable in helping me make an informed decision about this purchase. Thank you in advance for sharing your expertise and feedback.
Best regards.
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The LCMS-9030 quadrupole time-of-fight (Q-TOF) mass spectrometer integrates the world’s fastest and most sensitive quadrupole technology with TOF architecture. A product of Shimadzu's engineering DNA, speed and effortless performance enable the LCMS-9030 to address qualitative and quantitative challenges with genuine confidence and ease.
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Has anyone purchased or used the table top BD Facs melody cell sorter? Our lab purchased one 1.5 years ago and it has been the most frustrating machine ever. The stage locks up, techs have to come out every 2-3 months to fix something and reboot the software, etc. I am trying to ascertain other researchers experiences , if any, with this model. I believe we have an absolute lemon but I want your feedback.
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Hi Leah,
my feedback may be a bit late. But I've slowly made friends with this machine. :-) I think the software is simpler than BD Diva, but you can't change much about the basic settings. Unfortunately, a technician from the company has to come for this. Otherwise, it's going very well. Personally, I clean the Flowcell excessively. This means that I perform up to 8x "FlowCell-Washes" before the CS&T beads runs. And since our machine is not in continuous operation, I always perform the long-term shutdown and then the machine is in EtoH.
Are your experiences with BDMelody are better now?
Best Claudi
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This year we have the opportunity to purchase an HRMSD. We have an Agilent Infinity II 1260 HPLC in our lab and are planning to purchase a Q-TOF. I have experience with Agilent equipment (GC/LC/GC-MS SQ/LC-MS SQ), but not with HRMS.
We are planning to use this instrument for untargeted metabolomics. We are interested in searching for producers of new antimicrobial compounds, performing screening and identification of new antimicrobials of microbiological origin, and studying the biosynthesis of microorganisms.
So far, we have been offered two Agilent detectors:
1. 6546
2. Revident.
As I know HRMS produces huge amounts of data, and performing untargeted metabolomics workflow requires additional software to work with the data in case of untargeted analysis.
Now Agilent offers two sets of software, which is a bit confusing for me.
1. Let's call the first set "classic". It includes:
a. MassHunter Profinder (for Feature finding stage)
b. Mass Profiler Professional (MPP) (for Alignment and statistics)
i. ID Browser (module of MPP for Identification)
ii. PathWay Architect (optional MPP module for metabolite pathways buildings)
c. METLIN PCDL for LC/Q-TOF (database for metabolomics)
2. Let's call the second set "new" It includes:
a. software product - MassHunter Explorer, which, according to the manufacturer, combines all of the above software products into one.
b. ChemVista library manager with METLIN PCDL library
Questions:
1. Is the MassHunter Profinder standalone SW or is it part of MassHunter Qual or Mass Profiler Professional
2. Which one of the SW sets should be chosen? They do the same. But do they really do the same and have the same capability? Marketing? From my experience the early version of SW is quite restricted. For 6546 and the newest Revident Aglent recommends MassHunter Explorer.
3. To buy or not to buy:
a. I read that untargeted analysis has a huge community and freeware databases and SW for metabolite identification. Is METLIN PCDL library a MUST part of SW from the Vendor? Can I consider it as optional and use freeware DB?
b. The same question about ChemVista library manager?
4. By which SW/Databases do you realize your untargeted metabolomics workflow?
5. Any experience with the Revident model of Q-TOF. How far is it better/worth in metabolomics compared to 6546? Marketing?
a. Intelligent functions
b. Solutions for Tuning
6. Is the APCI source essential for untargeted metabolomics?
Thank you in advance.
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Dear Andriy,
First, congratulation with your future instrument. I personally love Agilent 6546, I did not work with Revident though.
Second, in my opinion, the most important step in untargeted metabolomics by HRMS is to fully understand what it can and can't do. Basically, I think a researcher has to accept that untargeted metabolomcis will not provide meaningful results immediately. Also, one has to accept that a whole process is so complex (not difficult, but complex), that it will be sub-optimal for most of the chemicals of interest. Therefore, for example, I would not consider APCI as a must have source. In contrast, for the targeted analysis, APCI can be essential in some cases.
The software provided are kind of repeat each other (in my impression), so I ended up using mostly MPP for all tasks. I think it is quite nice, intuitive and powerful. I do prefer to perform post-processing data analysis using external soft, but build-in functions work well for the initial results.
I do recommend to have PCDL, it can save you a lot of time for the primary metabolites annotation on the fly. But you can use external soft and databases too, if PCDL is beyond your budget or so.
Maintenance of 6546 is acceptable. in my case I have to prepare tuning mix by combining purchased components, but I heard Agilent is going to (already did) provide prepared mixes. Cone cleaning is easy, the needle adjustment can be tricky, but hopefully you will not need it often. I also like Agilent's video instructions., but I strongly recommend you to make sure how good is Agilent support in your location. Problems are unavoidable, I had a great support in this cases, but it is in the US. I know that other locations can be different.
Good luck.
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i want to know your thoughts on this topic and how i should tailor mt research.
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so i have changed by topic to influence of emotional advertising on purchasing decisions: no need to add milk milo 3 in 1 advert. i was asking what your thoughts are the topic.
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I am looking for a small (approx. 1 cm2) wireless (preferably Bluetooth) potentiostat/potentiometer for measurements in closed wounds, e.g., implantable potentiostat. At first, I am interested in the simplest potential measurements (range from - 400 mV to 200 mV, measurements for 1-2 weeks in implanted situation). Let me know if you have such or know where to purchase.
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Thank you. Sensit look small, but I am looking for implantation i rats.
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We are trying to dry trace amount of water from anhydrous acetone we purchased because we found that it still reacted with our moisture sensitive samples. Thanks.
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To dry a trace amount of moisture in acetone, one can employ azeotropic distillation by using ethyl bromide as a separating agent, which can give effective separation.
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I would like to purchase a good grinder for this
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Freeze the leaves in liquid nitrogen or use a freeze dryer. This step helps in preserving the isotopic composition of the samples.
Alternatively, you can use an oven or a desiccator to dry the leaves, but be cautious as some isotopes may be affected by high temperatures.
After this step you can grind with any available grinding equipment.
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I noticed that many researchers compared electrocatalysts for hydrogen evolution and oxygen evolution reactions with the activity of 20%Pt/C and RuO2, respectively. Could you please tell me how to prepare those electrodes or recommend a suitable supplier for purchase? When I visited the Sigma Aldrich website, they only had 10% Pt/C electrodes. I would greatly appreciate it if you could respond or offer advice in this regard.
Thank you
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You can use 10%, because it is commercial standard and you need to compare your work with commercially available catalyst
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I am looking for tools to measure the mindfulness and wellness among health care workers. It seems that FFMQ or FFMQ15 are commonly used to measure mindfulness.
Questions:
- Is there a purchased license required for use the FFMQ tool(s)?
- Anyone used the FFMQ or FFMQ15 previously? It seems that internal consistency and sensitivity to change are adequate for both versions. What was your experience with data analysis?
Gu J, Strauss C, Crane C, Barnhofer T, Karl A, Cavanagh K, et al. Examining the factor structure of the 39-item and 15-item versions of the Five Facet Mindfulness Questionnaire before and after mindfulness-based cognitive therapy for people with recurrent depression. Psychol Assess 2016;28(7):791-802.
Kind regards,
Grace
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The FFMQ-15 is under CCBY 3 copyright , which means you can use This article has been published under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright for this article is retained by the author(s). Author(s) grant(s) the American Psychological Association the exclusive right to publish the article and identify itself as the original publisher
See link
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the research objective is "Identify the key factors of brand equity that influence the decision-making process of young female customers when selecting a clothing store to purchase clothing items."
And also, I have a question, that how do the price, quality and style impact to the brand equity of clothing stores.
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Try with piloting a qualitative study to get closer to the comprehensive model. The factors to extract depend on the context.
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I am looking for a mammalian cell media with a few specific supplements, but it is not available for purchase from any vendor. The lab-grade supplements are pretty expensive and are sold in either huge tubs or in tiny amounts. I thought to just buy the required supplements (L-arginine and L-lysine) in the pharmacy and add appropriate amount to my media. Do you think it is ok? I shouldn't list the source of supplements when it is time to publish? The pack size and purity of pharmacy-sold supplements seem to be suitable for my experiment.
Cheers,
Maria
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Whatever you end up doing, not listing the source would not be OK. Think about it, if someone had to replicate it, this could be an important variable that needs to be considered. You should always disclose your experimental methods as much as you can, replication is crucial in science.
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Just anybody who has an interest in generic marketing issues
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Fodouop Kouam Arthur William Elena Atrushkevich I appreciate. Kindly link up with me on whatsap +263714953977 so we discuss the nitty gritties.
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I am searching for antibody to rat brain tissue. I would prefer to purchase antibody for both Western blot and Immunohistochemistry.
Thank you in advance.
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Hi, yes l could recommend an antibody to the GIRK4 protein encoded by the Kcnj5. I would probably recommend either the Anti-GIRK4 (KCNJ5) antibody from Abcam or the Anti-KCNJ5/GIRK4 antibody from CST. Both of these antibodies are specific for the GIRK4 protein, which is encoded by the Kcnj5 gene.
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The purchased EVA (VA content 40%) product is dissolved in toluene solution, and then a mixture of sodium hydroxide and alcohol is added, the concentration is about 1mol/l and 0.25mol/l. The infrared test results of the product are normal, but it has been unable to dissolve in DMSO. There is no major research in this area can answer or discuss. Whether the EVA synthesized by oneself and then make EVOH will dissolve in dmso better.
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EVA it is not soluble in polar solvents, that is why it is sold in Toluene. The only reference that I have it is that is soluble as much as 8% concentration of anhydrous dimethyl sulfoxide (DMSO).
Best regards
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Hi all,
Does anyone know where I can purchase a TEM sample rod holder that works with FEI/Thermo Scientific TEM rods and allows for 360­° rotation to check and grease the O rings?
I have an example image attached. I shows a sample rod holder that Fischione provided with our tomography holder. This one doesn't work for double tilt holders, however.
I don't know why it's so hard to find this basic but fundamental accessory. JEOL seems to provide these with their holders. I don't know why FEI/Thermo doesn't, but it's causing all kinds of vacuum troubles not being able to easily check and grease the O rings. Thanks!
Sheri
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It should be a simple task for a mechanical workshop of an university to build a similar tool or modify the existing one (if the black parts are fixed by screews). An original part would be xk€, my guess.
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Hello all. We are trying to get access to prostate cancer cell lines that were derived from patients who were Black or of African descent. There are numerous relevant cell lines reported in the literature, but they are hard to find for purchase - the only one we have found for sale is MDA PCa 2b / CRL-2422. Would anyone be able to provide a link to any others that are for purchase, or else be willing to share a flask or vial with us if you have some in your lab? We have reached out to some authors who developed relevant cell lines, but unfortunately haven't heard back yet.
Many thanks!
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Thank you very much! Yes, this is on our list (shared below) but unfortunately the only cell line out of these that we've been able to find available for purchase is MDA-PCa-2b. Do you know where we can get access to the cell line you mentioned?
  • MDA-PCa-2a
  • MDA-PCa-2b
  • P69SV40T
  • RC-77T/E
  • AA-103B
  • iHGPINc
  • S006AA
  • E006AA
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I recently purchased a vial of Cynom-K1 cells. I grew them up over several weeks as they were of VERY poor viability when they showed up. Expanded them, froze back 35 vials, saved a couple million and did a DNA extraction. Amplified an intron from a region for which I had Cyno and Rhesus sequence to confirm that these were actually Cyno cells. To my surprise they were HUMAN. A warning to anyone getting in cells, make sure you know what you have before starting any work with them.
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What's this underselling yourself about? Taking a lesson and learning from it. That's the best you can ever do. I think that's exactly what you get full credit for.
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Hi All,
I'm wondering if anyone knows where I could purchase a physical copy of the "Journal of Comparative Neurology: Research in Systems Neuroscience, Volume 524, No. 16 (November, 2016)". Picture attached.
Thank you for your help with this!
Kind Regards,
Ariel
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Dear Ariel,
Good luck,
Martijn
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Could you please assist me in finding survey questions related to influencer marketing in Uzbekistan? I'm particularly interested in understanding consumer perceptions and behaviors influenced by influencer characteristics, cultural factors, and the competitive landscape. It would be helpful to have questions that explore the impact of influencers' attractiveness, credibility, trustworthiness, and exposure on consumer brand perception and purchase intentions. Additionally, questions that address the challenges faced by marketers in this context, such as cultural nuances and navigating the competitive digital marketing industry, would be valuable. Thank you for your assistance in providing relevant survey questions for this topic.
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I send an article with relevant questions on the topic. I hope it helps.
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Explanation:
Only 57% of the expenses for urban trains and 40% for suburban trains have been recuperated, a fact that regular train travelers might have observed through ticket prices.
Despite these challenges, Indian Railways has achieved a remarkable milestone in the 2022-23 fiscal year, reporting a record revenue of ₹2.40 lakh crore. This represents a substantial increase of almost ₹49,000 crore compared to the previous year, as highlighted in a ministry statement on Monday. Notably, the freight revenue experienced a significant uptick, reaching ₹1.62 lakh crore, marking a robust growth of nearly 15% from the preceding year, as reported by NDTV on April 18, 2023.
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One can see if it is possible to serve tea/ drinks/ snacks with some extra luxurious facility to 2nd ac and 1st ac from irctc like rajdhani. Which price should not be included in ticket. It should be separate. And there should be magazine which people can take ri read for some price and return back. News paper facility. Several times there is an internet issue, so there may be chargeable wifi.
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Does anyone know where I could purchase gold-core silver-shell nanoparticles in a dichloromethane solvent? Our usual supplier only seems to have them in water.
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Thank you Chen, I will be sure to check it out with them
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Hi
I'm working on cell imaging by Raman mapping.
Recently, I purchased the water immersion lens and measured the cells in the PBS.
When I use water immersion lens I often get some lines in the result after processing data, which did not appear before.
What I thought the problem is the water immersion lens but I can't sure about this.
Is there any opinion can help me?
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thanks, you are welcome
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I am currently a senior high school student, and I consider myself a beginner in this field. We are tasked to conduct a qualitative study about the effects of influencer marketing on purchase behavior. I am interested to know and hear what theories or theoretical literature that can be applied in our research.
Thank you in advance!
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There are lots of researches on influencer marketing and purchase behaviour. Just search on the internet and take the thorough review of past researches. You will get the guideline regarding what to do? How to do? Find out conclusions and suggest recommendations. There is no shortcut for quality research. You will have to give time and efforts, but the result will be such that you can proudly say anybody I done genuine research. I hope it will be useful to you.
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I have a case in my field research I want to know the consistency of the fish troops at Market X I recorded data for 7 days (from August 11 to August 17) regarding: (A) Fish sold by traders at Market X (B) Fish purchased by consumers at Market X The respondents I interviewed were 30 traders at Market X I conducted a census of the same 30 traders for 7 days (from August 11 to August 17) regarding: (A) Fish sold by traders at Market X (B) Fish purchased by consumers at Market X What analysis is suitable to be used to provide an overview of the consistency of fish supply in Market A phenomenon that is clearly visible from the census results is that the types/variations of fish purchased by consumers are less than the supply of types/variations of fish sold by traders. Do any of you have a case like mine?
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@all a case where you want to assess the consistency of fish supply in Market X, particularly comparing the types/variations of fish sold by traders (A) and the types/variations of fish purchased by consumers (B). Additionally, you've noted that there is a discrepancy between the supply and demand in terms of the variety of fish types. Here are some suggested analyses to provide an overview of the consistency of fish supply in Market X:
  1. Descriptive Statistics:Calculate summary statistics for both variables (A and B) for each day. This includes measures such as mean, median, mode, range, and standard deviation. This will give you an initial sense of the central tendency and variability in fish supply and demand.
  2. Daily Comparisons:Create daily comparisons between the types/variations of fish sold by traders and those purchased by consumers. Identify any patterns or discrepancies over the 7-day period.
  3. Consistency Index:Develop a consistency index to quantify the level of consistency between the types/variations of fish sold and purchased each day. This could involve calculating the proportion of overlapping fish types or using other indices that highlight the degree of similarity.
  4. Bar Charts or Graphs:Create visual representations, such as bar charts or line graphs, to illustrate the daily trends in fish supply and demand. This can help identify any noticeable patterns or variations over the 7-day period.
  5. Statistical Tests:Consider using statistical tests to assess the significance of differences between the types/variations of fish sold and purchased. Depending on the nature of your data, you might use t-tests, chi-square tests, or other appropriate statistical tests.
  6. Market Dynamics Analysis:Explore the dynamics of the market by examining whether certain types/variations of fish are consistently popular among consumers or whether traders are introducing new varieties. This analysis can provide insights into market trends.
  7. Qualitative Data Analysis:If applicable, analyze qualitative data from interviews or open-ended survey questions. This can provide additional context and explanations for any observed patterns or discrepancies in the data.
  8. Longitudinal Analysis:Conduct a longitudinal analysis to examine trends over time. This involves looking at how the consistency in fish supply and demand evolves throughout the 7-day period.
Remember, the choice of analysis depends on the nature of your data and the specific research questions you want to address. Combining quantitative and qualitative approaches can provide a more comprehensive understanding of the consistency of fish supply in Market X. Additionally, consulting with a statistician or data analyst for more advanced statistical methods might be beneficial based on the complexity of your dataset.
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We are currently conducting a qualitative research on the effects of influencer marketing on purchase behavior. However, before that, our panel has suggested to conduct a pre-survey to (1) identify the products endorsed by influencer marketing that students mostly purchase, and (2)the social media platforms that students purchase influencer-endorsed products. The purpose of the pre-survey will help us narrow down our scope based on the results of the pre-survey by focusing on a specific social media platform and product.
Our question is how can we determine the sample size?
Thank you in advance!
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RV Krejcie and DW Morgan,Educational and Psychological Measurement, 1970,30:607-610, gave a table to choose sample size. Also MA Hertzog ,Research in zNursing and Health,2008,31:180-191. Suggested that sample sizes should be higher that 40. REGARDS.
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Hi, i am looking for a survey questionnaire which involves the marketing mix strategy and purchase decision? Help will be appreciated if anyone came across this question on researchgate. THank you very much!
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You may want to check relevant research papers published in sound academic journals to get ideas on their research questionnaires.
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I am currently a senior high school student, and I consider myself a beginner in this field. We are tasked to conduct a qualitative study about the effects of influencer marketing on purchase behavior. I am interested to know about purchase behavior for our research project in marketing. Is there any literature that may help? May I ask what is purchase behavior and how does it differ from consumer behavior?
Thank you in advance!
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Purchase behavior, often referred to as consumer buying behavior, is the process through which individuals or organizations make decisions to acquire products or services. It encompasses a complex interplay of psychological, social, and economic factors that influence the choices people make when buying goods or services.
At its core, purchase behavior is driven by the needs and desires of consumers. These needs can be functional, such as the need for food or shelter, or emotional, such as the desire for luxury or status. The process begins with problem recognition, where a consumer identifies a need or a problem that can be solved through a purchase.
Various factors shape purchase behavior, including personal factors like age, income, and lifestyle, which influence what products or services an individual is likely to consider. Social factors, such as culture, family, and reference groups, also play a significant role. Furthermore, psychological factors like perception, motivation, and attitudes contribute to the decision-making process.
Economic factors, such as price, income, and availability, are critical determinants in the final purchase choice. Marketers and businesses carefully study purchase behavior to understand their target audience and tailor marketing strategies and products accordingly. Ultimately, purchase behavior is a dynamic and multifaceted field that explores the intricate processes behind consumer decision-making and its impact on the market.
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I am trying to analyse the polyphenolic or flavonoid content of my honey sample. Can I do it with some new method that doesn't require purchasing any standard for chemical compounds?
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Hello.
You can get structural information on PCs by comparing retention times and UV spectra with the literature. Please check the following books:
Campos, M.G., & Markham, K. R. (2007). Structure information from HPLC and on-line measured absorption spectra: flavones, flavonols and phenolic acids. Imprensa da Universidade de Coimbra/Coimbra University Press.
Mabry, T., Markham, K. R., & Thomas, M. B. (2012). The systematic identification of flavonoids. Springer-Verlag.
Regards.
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Hi everyone.
I'm in the process of purchasing an stereo-microscope of the Amscope brand, specifically an SM-2T, but I ran into this thought of what if this microscope is not suitable for what I'm looking for?. I searched for academic papers and, indeed, some of them report using this brand but different models and magnification range. The equipment I'm purchasing has 7x-90x magnification range, a 2x magnification barlow lens, and a 5MP camera attached. So... does anybody has this microscope and work with microplastics by any chance that could lead me in my purchase or has any experience in observing very tiny microplastic-like objects with this equipment? It'd very helpful for me to avoid making a bad acquirement.
Thanks for your time and attention.
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I haven't used the SM-2T, but other Amscope models I've seen are decently good and some parts are interchangeable with other Amscope models. It should be fine for microplastics unless you want to get down to the tinier than dust sized particles. Get the LED illuminated one rather than halogen so that you won't have so much color shift as the source brightness is varied. The LED runs cooler and lasts a lot longer.
The 2X converter is probably more convenient to shift in and out but I wonder if your viewing quality would be better by having a pair of 20X oculars on hand? OTOH, the camera might not be seeing the same when one only changes the oculars, so if you will be taking many photos, the teleconverter might be better.
Make sure the camera works with the camera port. I've run into one setup that the camera worked fine in the ocular tube but not the camera tube. Could've been the wrong optics in the camera although the model was correct.
Consider if you want polarized lighting or different spectra for better contrast. Think about how you will control static as some microplastics will fly around and stick to various surfaces unless you have them contained.
You may be able to find a good used one if your budget is tight.
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Hello everyone,
I would like to purchase male microneedles. Would you please introduce me a company which make them? Thanks.
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Thank you for your response but when I talked about male microneedle I mean "microneedle master molds" ( the master molds are used to make silicone templates, not whatever could be found by online marketplaces).@sabur setiaman
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Bunker fuel suppliers around the world have the tendency of delivering less than what they state in their Bunker delivery not (BDN), leading to commercial losses for the purchasers
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I can't say that I'd heard of this issue before. Certainly monitoring fuel oil flows accurately is not easy as their chemistry and physics vary widely. Flow totalisers are available but only measure volumetric flow, so would not guarantee the energy purchase value. I do not know what contract forms are used for large scale oil purchases, but am sure that much effort goes into them!
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We can purchase poly-lysine coated coverslip but they have an expire date. Could we coat again with poly-lysine some expired coverslips?
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Coating a coverslip that has already been poly-lysine coated, even if it has expired, is possible in some cases, but the success of the coating may be compromised.
Poly-lysine is often used to promote cell adhesion in cell culture or microscopy applications, and its effectiveness can diminish over time, especially if it has expired.
Here are some considerations:
  1. Expiration Date: Check the expiration date on the poly-lysine coating solution. If it has expired, the chemical properties of the solution may have changed, which can affect its ability to coat surfaces effectively.
  2. Storage Conditions: The effectiveness of the coating can also be influenced by how the solution has been stored. If it was stored improperly, such as exposure to extreme temperatures or light, it may not work as expected.
  3. Cleaning and Re-coating: If you still want to attempt to coat the coverslip, you can try the following steps: a. Clean the Coverslip: Ensure the coverslip is thoroughly cleaned to remove any remnants of the old coating. You can use a suitable cleaning solution (e.g., ethanol or isopropanol) and rinse it with distilled water. b. Prepare Fresh Coating Solution: If possible, prepare a fresh poly-lysine coating solution following the manufacturer's instructions. Using expired reagents may result in inconsistent or unreliable results. c. Coat the Coverslip: Apply the fresh poly-lysine solution to the coverslip and incubate it for the recommended duration and conditions specified in the protocol. Make sure the coverslip is evenly coated.
  4. Testing: After coating, you may want to test the effectiveness of the coated coverslip with a simple cell adhesion assay or a microscopy experiment to see if it provides the desired results. If the results are unsatisfactory, it may be necessary to obtain a new, non-expired poly-lysine coating solution.
Keep in mind that using expired or compromised reagents can lead to unreliable or inconsistent results in your experiments. It is generally advisable to use fresh, unexpired reagents for critical experiments to ensure the best possible outcomes.
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Please suggest diet analysis software for 24-hour dietary recall and the price of purchasing the software.
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National Cancer Institute's Automated Self-Administered 24-Hour Dietary Assessment Tool
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I am looking for guidance on stratified sample sizes. I would like to analyse buying behaviour of businesses within a total population of 100,000.
There are 12 different types of businesses within the population. Each have different behaviours when it comes to what they buy, what volume they purchase, and how frequently.
I want to survey these businesses to analyse their different behaviour.
I know the total population, and the population of each business type.
I want to achieve high accuracy, but within a budget.
So my question is: what is the sample size?
Is it the statistically correct sample size for the total population, stratified by the business types?
Or is it the statistically correct sample size for each business type, added together?
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To ascertain the appropriate sample size for your investigation into the purchasing behavior of businesses within a total population of 100,000, categorized into 12 distinct business types, it is imperative to calculate the sample size independently for each stratum (business type) and subsequently aggregate them. This systematic approach guarantees the precise capture of behavioral diversity within each stratum.
Here is a stepwise guide for determining the sample size for a stratified sample:
  1. Establish Stratum Proportions: Commence by computing the proportion of each business type within the overarching population. This calculation involves dividing the population count of each business type by the total population.
  2. Estimate Variability: Obtain an estimate of the variability inherent to each stratum. This estimation can be founded on historical data, pilot studies, or informed assumptions. The level of variability will exert an influence on the requisite sample size.
  3. Specify Confidence Level and Margin of Error: Define your preferred confidence level, such as the commonly chosen 95%, along with the acceptable margin of error, which signifies the degree of permissible variation.
  4. Compute Sample Size for Each Stratum: Utilize the prescribed formula for sample size computation, employing it individually for every stratum. The formula is contingent upon the designated confidence level, margin of error, and the estimated variability within each stratum.Sample Size for Each Stratum = (Z^2 * p * (1-p)) / E^2Z represents the Z-score pertinent to your chosen confidence level. p corresponds to the estimated proportion of the stratum within the population. E signifies the margin of error.
  5. Repeat the Process for All Strata: Apply the formula iteratively to each of the 12 business types, leading to the calculation of the requisite sample size for every stratum.
  6. Aggregate the Sample Sizes: Conclude the process by summating the individual sample sizes obtained from each stratum. This cumulative figure represents the total sample size requisite for your study.
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Dear All,
I did the extension experiment with 20mer RNA primer annealing with 40mer RNA template in the presence of recombinant human coronavirus SARS-CoV-2 RNA-dependent RNA polymerase (RDRP) which was purchased from the vendor. No extension product was observed but some products which were lower than primer shown were found (please see the attached screenshot). Does anyone know the possible reason to cause this? The primer and template were purchased directly from IDT and they are still good (I checked the purity via HPLC). And the polymerase was purchased in July and are stored under -80C until using. Thanks in advance!
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RNA Polymerase should have 3’-5’ exonuclease proof reading activity and it is simultaneously degrading the primer and extending it with ATP. If the exonuclease site probably glutamates and aspartates that bind divalent magnesium are mutated to alanine this should knock that out.
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Having a bit of trouble with working out some calculation's.
I have purchased 1mg PMA and plan to dissolve in 1ml DMSO to get a concentration of 1mg/ml.
My plan is to aliquot thESE and freeze. Perhaps 1ul in each vial?
However i am unsure of how many mls of media to add to each aliquot to give the final concentrations of 2.5ng/ml, 5ng/ml, 10ng/ml, 20ng/ml and 50ng/ml?
Probably quite a simple calculation but any help would be greatly appreciated!
Thank YOU
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thanks so much for your quick reply
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Hi!
I am trying to purchase the protease MMP21 but wasn't able to find a commercial source. Does anyone know of a distributor? I am located in the US.
Thank you!
Nadine
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Hello Nadine,
You may buy it online. You may make an inquiry at Alfa Chemistry, they offer kinds of good-quality products.
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Please how much will it cost me to purchase primers for infectious laryngotracheitis? and where can i purchase these primers?
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the web page of Macrogen gives you the price and quotation at the moment, also they produce very good primers and they deliver your the package within 1 week. The best service in my opinion. im from latin america!
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I used to work with a PM-IRRAS + Langmuir balance from KSV NIMA but it is no longer available. I want to purchase a PM-IRRAS module compatible with Langmuir balance, does anyone know another company or have experience with other similar setup?
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Some companies that are known to provide surface analysis and spectroscopy instruments, which might include PM-IRRAS modules compatible with Langmuir balance setups, are: BioNavis, Harrick Scientific, Horiba (Horiba offers a range of spectroscopy and surface analysis instruments. While they might not offer an integrated PM-IRRAS + Langmuir balance setup, they might have compatible components that can be assembled into a similar system.), JASCO, SensiQ Technologies (SensiQ specializes in surface plasmon resonance (SPR) and other surface analysis technologies. While not exactly PM-IRRAS, their expertise in surface analysis might lead to related solutions.)
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I am looking for large data sets of medical imaging, mostly related to neurology. Specifically, a wide array of CT, MRI, diffusion, CTA, PE, and the like. I was wondering if anyone can share some companies they may be aware of that sell such datasets?
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  1. The Cancer Imaging Archive (TCIA): TCIA is a well-known and freely accessible resource that provides a large collection of cancer-related medical images, including CT, MRI, and PET scans.
  2. Radiological Society of North America (RSNA): RSNA offers image datasets, including CT and MRI scans, for research and educational purposes. Some datasets may be available for purchase or access.
  3. Open Access Medical Imaging Repository (OAMIR): OAMIR is a collection of openly accessible medical image datasets that researchers and students can use for various purposes.
  4. Kaggle: Kaggle is a platform that hosts data science competitions, and occasionally, medical image datasets are part of these competitions. You can explore Kaggle's datasets section to find medical imaging datasets.
  5. PhysioNet: While primarily focused on physiological data, PhysioNet also hosts some medical image datasets for research purposes.
  6. DICOM Library: DICOM Library provides a selection of freely available DICOM (Digital Imaging and Communications in Medicine) images for educational purposes.
  7. Medical Image Data Open Resources (MIDORI): MIDORI is an initiative that curates openly accessible medical image datasets.
  8. National Institutes of Health (NIH): NIH and various other research institutions might occasionally release medical image datasets for specific studies or research projects.
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Thanks to all replies.
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And to add to David L Morgan 's very useful reply, please do not ask people to do unethical things like provide you with software you didn't pay for. Software developers have to earn a living too.
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Unethical business practices operate in different countries to varying degrees and participate in economic processes.
For many entities, market participants, business partners and consumers, they generate additional costs.
They can also be a source of gray economy growth, including avoiding paying taxes.
Thus, there are social costs for individual entities and financial for the entire economy.
On the capital markets, one of unethical business practices is, for example, insider trading, ie the use of confidential information by decision-makers with access to confidential information used to conduct transactions to purchase or sell financial instruments, including securities or other securities or other capital markets.
In individual countries, there are various instruments to combat the use of unethical business practices, the shadow economy, etc.
The effectiveness of individual normative solutions, the scale of restrictions applied, and the business mentality of market participants, entrepreneurs and businessmen are different.
Another mentality is related to the level of awareness regarding corporate social responsibility.
In individual countries, social campaigns are carried out suggesting the legitimacy of developing concepts based on corporate social responsibility.
In view of the above, please answer the following question: Is unethical business practices a negative external effect of non-ideal market structures or imperfection of the social market economy?
Please, answer, comments. I invite you to the discussion.
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Imperfections of the social market economy as unethical business practices do reflect human behavior, i.e. not external to market structures as the market should not be idealized as perfect answer to satisfy human needs.
As the old police officer saying at shift change goes: be careful out there !
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What Makes Someone A Great Entrepreneur?
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Discovering gaps in the market by meeting unmet human needs makes one a great entrepreneur, dear Kwadwo Boakye The great entrepreneur is not a speculator for temporal (short.term) extra-profit by buying and selling, but s/he cares for (long-term) business customer relations.
A very methodical life-style and monetary expertise are paramount to entrepreneurial success.
„A piece of knowledge about boat-building, about whose correctness Crusoe has no doubts at all, will not be seen as a hunch and will be valued according to Menger's Law. It may be said that Crusoe is well aware that he possesses this kind of information; he will deploy and value it in the same way as he may be imagined to deploy and value other resources he believes are definitely at his disposal. But concerning Crusoe's hunches and his visions in the face of a changing, uncertain environment, it cannot be said at all that Crusoe knows he has a hunch or a vision of the future. He does not act by deliberately utilizing his hunch about the future; instead, he finds that his actions reflect his hunches…In other words, it turns out, the essence of entrepreneurial vision, and what sets it apart from knowledge as a resource, is reflected in Crusoe's lack of self-consciousness concerning it…Crusoe may…gradually come to be aware of his vision. When he does, that vision ceases to be entrepreneurial and comes to be a resource. Moreover, Crusoe's realization that he possesses this definite information resource may itself be entrepreneurial. As soon as he 'knows' that he possesses an item of knowledge, that item ceases to correspond to entrepreneurial vision; instead, as with all resources, it is Crusoe's belief that he has the resources at his disposal that may now constitute his entrepreneurial hunch.“ Israel Kirzner, (1979: 168-169); as cited from: Israel Kirzner's Entrepreneurship