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Purchasing - Science topic
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Questions related to Purchasing
I have purchased ellagic acid from himedia, but when i perform antimicrobial assay by agar well diffusion method, it's not showing antimicrobial activity at any concentrations. I have use 1mg/ml, 2mg /ml and 3mg /ml concentration for testing against bacillus subtilis, pseudomonas aeruginosa, and S. aureus. Why i am not getting any results?
I am trying to run a sulfatase activity assay using purified steroid sulfatase enzyme and 4-MUS as a substrate. The protocol I am following says to use 0.6 units of enzyme however the purified enzyme I have purchased did not come with a specified enzymatic activity (units). I'm not sure if this is a known figure or if I have to calculate this? any help is appreciated.
To measure The Impact of YouTube Advertisement Value on Purchase Intention of YouTube Users
Only 1 question in survey - less than a minute
Hello all, I am interested in purchasing a dox-inducible system that contains either GFP or RFP to transduce into cells and use for my animal studies. The cells will also contain luciferase as I will be detecting the growth of these cells in the animals using bioluminescence imaging. Will having GFP or RFP interfere with the bioluminescence signal and alter my results?
Does anyone know where to purchase research-grade Galleria mellonella in Europe?
I only find the TruLarv from BioSystems Technology, but it seems to be down...
Thank you!
I need to work in an arthritis model induce by serum from K/BxN. Do you know where can I purchase K/BxN serum?
For microfloatation with limited material availability
Dear all,
I have just purchased an aliquot of lncap from atcc, I thawed them and the result was disastrous. Briefly, these cells form big clusters and even when they adhere they maintain this morphology which seems far from the one in literature. Atcc told me that the RPMI I used (which is the same I employ for other lines) is too low in glucose, it omits sodium pyruvate and has HEPEs and sodium bicarbonate different from the one in the suggested RPMI. My ignorance, I wasn't aware that differences between RPMI from different suppliers were so important. In addition they told me I made a mistake using heat inactivated FBS as, probably, I eliminated some growth factors.
Could you kindly tell me if, in your opinion, are these requirements all mandatory for a satisfactory culture...it is so crazy. Thank you in advance
I purchased the Novex Native PAGE gels, did Native-PAGE electrophoresis, and continued transferring the protein to the PVDF membrane by western blotting. But I can`t see my protein marker from NativeMark™ Unstained Protein Standard (LC0725).
I have added 4x sample buffer or G250 additive to the NativeMark™ Unstained Protein Standard, but the result still showed no protein marker band.
I would appreciate your advice if anyone has any experience with Western Blot following Novex Native Page. Thank you very much.
Unfortunately the Clay Mineral Society (clays.org) no longer have any pure illite in stock, and we need some for some experiments. If anyone knows where to find some, or has any of the old IMt-1 or IMt-2 CMS reference materials gathering dust in their lab, I'd really appreciate the input.
Thanks all,
Michael
I purchased some alkali lignin a couple of days ago. From the literature study, I thought alkali lignin was not soluble in water. However, the alkali lignin I purchased is completely soluble in water, so I am confused about its solubility now.
Can everyone share their experience with the brand of micro-scissors, blade type (straight or curved), and affordability? Our lab only has one from F.T.S. 15004-8, and it's getting old. We repeatedly use it for mouse retina dissection, and we would like to purchase another one as a spare. I was wondering if there are more affordable brands out there that can do the same thing.
- Define Your Financial Goals: Determine what you want to achieve (e.g., retirement savings, home purchase, vehicle purchase, wealth accumulation, education funding, and children's marriage) and the time-horizon for each goal.
- Assess Your Risk Appetite: Evaluate your comfort with risk, considering your age, financial position, and emotional ability to handle market volatility.
- Understand Your Investment Horizon: The period for which you plan to invest impacts your investment portfolio. Longer horizons often favour riskier investments (like stocks), while shorter ones may require safer options (like bonds or Fixed Deposits or Money Market Instruments, etc.).
- Evaluate Your Current Financial Health: Take stock of your income, expenses, debts, and existing investments to understand how much you can allocate toward your portfolio.
- Diversify Across Asset Classes: Include a mix of assets such as stocks, bonds, real estate, bank deposits, bullion, AIFs, and cash to spread risk and improve potential returns.
- Consider Fees and Taxes: Factor like costs associated with investment (e.g., fund expense ratios, advisory fees) and tax implications to maximize net returns.
- Monitor and Rebalance Regularly: Imperative to periodically review your portfolio to ensure it aligns with your goals and risk appetite. You can make adjustments as needed owing to market changes or life events.
- Seek Professional Advice When Needed: If you're unsure about building or managing your portfolio, better consult a financial expert or use robo-advisors for customized recommendations.
What are the purchasable ligand databases which are currently also updated.
I have been looking into Zn purchaseable db through Pharmit server, it is really not updated and the ligands are no longer buyable.
Other than Zn I can think of FDA-approved ones. But are there any other database other that these two, taht has a buyable section?
Thanks
Sanjukta
I am currently conducting my undergraduate thesis and I need MRS agar and broth, however, the one accessible to me (that I am familiar with) is from HiMedia, which only comes in 500 g bottles (I only need like 100 g of).
Has anybody tried purchasing culture media from BaseBio (?) or Phygene (?)? I have never heard of these brands before which is why I am hesitant to purchase but they are the only ones that come in 100 g bottles.
Let me know how it went. Thank you!
We need to replace our large muffle furnace and the replacements are extremely expensive for the size we need. Can I purchase a high-end, vented kiln instead? Is there a difference in the two pieces of equipment that make them not interchangeable?
Hi all,
I am looking for type material of fossil terrestrial gastropods described by August Reuss. Specifically I want to find the syntypes of Vertigo callosa Reuss, 1849 from the Early Miocene of Tuchorice (CZ). Reuss worked, amongst others, in Prague and Vienna, and parts of his collections (microfossils) were purchased in 1891 by the Natural History Museum in Vienna. However, the mollusks aren't there, and I couldn't find any information on their whereabouts, apart from a few author's statements that the collection "might be lost". It remains uncertain though if anyone actually made a serious effort to locate it.
Any hint or suggestions would be most welcome!
Best wishes,
Thomas
How the backing plate can effect sputtering during process?
The cell line I plan to purchase from ATCC is not readily available. So the supplier suggested to try from iCell Bioscience, China. I have not heard of this cell supplier, so trying to see feedbacks and review from others.
I’m particularly interested in AI-driven simulation tools that can predict or model consumer behavior, focusing on areas such as purchasing decisions, market segmentation, or consumer preferences. If anyone has recommendations or has worked with such tools (besides the Consumer Behavior Spectrum Model), I would love to hear your insights!
Would it be possible to think that if there is a categorization regarding fundamental rights, the state should, in the same way, prioritize the budget, ensuring that the rights higher in the prioritization receive more resources? That is, why allow the state to allocate more resources to a policy that has to do with the disappearance of some autonomous organizations, regarding policies, infrastructure, projects that help minimize water waste, support the purchase of medicines? against cancer, among others. Wouldn't it be desirable to modify the way resources are allocated today?
The future of nuclear power depends upon several factors. One of these factors is the use of small module reactor (SMR) for electricity generation and for other non-electric applications. Several prototypes of SMR are under development in several countries, but only four of them at this moment are under construction in three countries. This type of nuclear power reactor will receive the approval of the public opinion of those countries thinking to expand or to introduce nuclear energy for electricity generation in the coming years and will allow the construction of this type of reactor in their countries? Which are these countries?
Use the Temu coupon code 'acs540599' to enjoy an incredible 90% discount on your next purchase. Plus, every order includes free shipping and hassle-free returns!
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Hi all.
My lab is starting chemotherapy drug sensitivity assays for oral cancer cell lines. We will be detecting cell viability with the MTT and SRB assays, both of which are fluorometric. Hence, we'll need to purchase a fluorescence microplate reader.
Which brand's plate reader/product is the best? We are looking into affordable options that work for 96 well plates and ideally other formats as well. Is there one you guys are using in your lab that is good and efficient?
It would be really helpful if I could be guided into making the right purchase!
I need to purchase paramagnetic magnetic nanoparticles ~500nm for magnetic manipulation and magnetic swarm formation.
Are there any companies that sell the product with international delivery (to South Korea).
I am planning to use prenyl binding protein PDED inhibitor Deltazinone 1 but I am unable to find any supplier?
Im working on fungal taxonomy,
I would like purchase suitable camera.
I welcome you guys to suggest best.
regards,
Niranjan
We wish to incubate and shake the 96 well plate, and measure the OD reading of bacteria.
The literature on Supply Chain Integration (SCI), Supply Chain Management (SCM), and collaborations, spanning several decades, deserves special attention. This topic, although addressed for a long time, remains contemporary and continues to be the subject of in-depth studies. The interest lies in understanding why collaboration in the supply chain remains a highly studied research topic, particularly concerning supplier relationships.
To achieve this, this study is organized around SCM and SCI, then purchases intended for sale in general, followed by strategic purchases, and finally the purchasing function in SMEs and its contribution to performance. The aim is to understand the purchasing behavior and competencies of SMEs, focusing on practices and examining their impact on value creation and long-term competitive advantage.
For this reason, this study aims to analyze supply chain management in a disrupted context. The objective is to discover how to optimize the purchasing function within an Extended Enterprise. This optimization seeks to identify actions to undertake to enhance its competitiveness and help it address the difficulties it faces, particularly against powerful suppliers who hold negotiating power, while considering its limited resources and competencies.
What is your perspective on this topic, both in terms of its relevance and the scientific validity of the potential results, and why?
Thank you
Nicolas
After lapse of a few months, we recently attempted insulin-tolerance tests in C57BL/6N female mice at 6 months of age. We began, years ago, with Humulin-r at 0.75 U/kg. In our recent tests we used the same protocol, but the only response was a slight increase in blood glucose! We tried several higher doses. At 1.75 U/kg a very slight decrease was observed, but not until 60 minutes. We purchased a non-clinical preparation of insulin from a company that sells a lot of growth factors and cytokines. It produced the puzzling increase in blood glucose at both 0.75 and 1.25 U/kg. Has anyone else had a problem with insulin efficacy of late? I can't believe all our mice are suddenly insulin resistant!
I have the Holtzman Inkblot Test Cards Form A, which I was able to purchase from an individual who had a set from 1958. I am now looking for Form B. I wish to do some research with this set of cards and cannot find them anywhere. If anyone has a set Form B I am willing to purchase them. My email is jspores@pnw.edu. Thank you.
In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and αSMA was very high, almost comparable to the TGF-β group. The culture medium for HK-2 cells is DMEM/F12 + 10% FBS + 1% PS. I am uncertain if I made any mistake that caused the HK-2 cells to start fibrosis on their own. If anyone could provide some advice, I would greatly appreciate it. Thank you!
Does anyone know if there is a lab scale encapsulation machine available for purchase?
Penconazole
1. CGA 132465
2. CGA 190503
3. CGA 127841
Fluopyram
1. AE C656948-benzamide
2. AE C656948-pyridyl-acetic acid
3. AE C656948-carboxylic acid
I have searched on sigma aldrich as well as on dr. erhenstofer site but i did not find any results.
We are normally using wet transfer for our mini gels for western. We would like to to purchase a new set-up for big gels (20x20cm), with this regard we would like to know which transfer system is better semi-dry or wet? Which would give us a more reliable result ? Time and money is not in question but the main concern would be the final outcome.
Thanks in advance for your help.
For example, when selling the service products, female seller has greater influence than male seller on customers' purchase intention. However, I want to find out when selling a normal product, female seller's strength was lower but not necessary be lower than male seller.
I was wondering whether there is a technique can help me to find out the "female seller's strength was reduced when selling the normal products when comparing to her own performance when selling the service product, but was always better than male sellers"? In other words, I want to find out "female seller's influence is always stronger than male sellers, but when selling different types of products, female seller's overwhelming strength would be fluctuate".
In this model, the moderator is the two types of products (service and normal product), which are coded as 0/1 variable. The antecedent is the two different sellers (female and male seller), which are coded as 0 or 1 variable.
I recently acquired a used Illumina HiSeq 2500 and am in need of flow cells compatible with this machine. As I am relatively new to this aspect, I would greatly appreciate any recommendations on reliable suppliers or vendors where I can purchase these flow cells.
Thank you in advance for your assistance.
HUMOROUS MORPHOLOGY
In Lewis Carroll’s Alice’s Adventures in Wonderland, the Gryphon used the word “uglification,” and Alice asked the Gryphon what the word meant. The Gryphon was amazed that Alice didn’t know this word. He asked Alice if she knew the meaning of “beautification,” and Alice responded that she did, but she still didn’t think that “uglification” was a real word with a real meaning. Indignantly, The Gryphon said that a word means whatever you want it to mean, and then he went on to give other examples. He said that what is taught in the schools is “reeling,” “writhing,” “uglification,” and “derision.”
Teachers should be called “tortoise” because “they taught us.” Lessons are called “lessons” because they lessen every day. In Wonderland, “Latin and Greek” are called “Laughing and Grief,” and “drawing, sketching and pointing in oils” becomes “drawling, stretching, and fainting in coils.”
The Watergate Hotel is where the break-in of the National Democratic headquarters occurred. Today’s dictionaries give more room to the metonymous meaning of Watergate than to the literal meaning of “a gate controlling the flow of water.” “Gate” has now become a suffix meaning “scandal” as in Irangate, Contragate, Iraqgate, Pearlygate, Rubbergate, Murphygate, Gennifergate, Nannygate, Monicagate, ad infinitum.
On National Public Radio’s “Cartalk,” Click and Clack are playing with Morphology in their list of credits: Copyeditor: Adeline Moore, Accounts Payable: Ineeda Czech, Pollution Control: Maury Missions, Purchasing: Lois Bidder, Statistician: Marge Innovera, Russian Chauffeur: Picov Andropov, Legal Firm: Dewey, Cheetham, and Howe.
Another kind of humorous morphology is when words are translated (actually transliterated) from one language to another. “Un petit d’un petit / S’étonne aux Halles” makes no sense in French, but it makes perfect humorous sense in English: “Humpty Dumpty / Sat on a wall.”
In summary, the inflectional and derivational morphology of English Nouns, Verbs, Adjectives, Adverbs, and Pronouns have an amazing capacity to morph into other Nouns, Verbs, Adjectives, Adverbs, and Pronouns.
Can you think of other humorous examples of English words morphing into other words?
Don and Alleen Nilsen “Humor Across the Academic Disciplines” PowerPoints:
I am unable to find them on common cell banks like ATCC. Thank you!
According to my latest co-authored research with Dr Dharmendra Naidu that is published in a prestigious journal, “The Accounting Review”, TAR:
“We find that customers’ financial reporting quality is positively associated with future supply contracts, indicating that suppliers are more willing to contract with customers who provide them with better information”, (Naidu and Ranjeeni, 2024).
Pleased to share that our research is available for free downloading and reading for a limited time. Please access our published paper in TAR using the DOI below:
Abstract
Using a unique hand-collected dataset of purchase obligations, we find that customers’ financial reporting quality is positively associated with future supply contracts, indicating that suppliers are more willing to contract with customers who provide them with better information. Further, the association between customers’ financial reporting quality and future supply contracts is stronger for customers with strong bargaining power, which is consistent with suppliers relying more on financial reports when they have less access to customers’ private information. Collectively, our results suggest that customers’ financial reporting quality plays an important role in influencing suppliers’ decisions to contract with customers for the future supply of goods and services.
Reference:
Dharmendra Naidu, Kumari Ranjeeni; Is Customers’ Financial Reporting Quality Associated with Suppliers’ Decision to Contract?. The Accounting Review 2024; https://doi.org/10.2308/TAR-2021-0652
The fluorescent labelled primer for the expected sample is purchased 2 years ago and for the sample with intense signals is purchased 3.5 years ago and have been kept in the identical condition ever since.


This concise research question focuses on two main aspects:
- Impact of Augmented Reality (AR) on Purchasing Decisions: The question investigates how the use of AR technologies in online luxury fashion retail platforms affects the buying behavior of consumers in the UK. AR can offer immersive experiences where consumers can virtually try on clothing or accessories, enhancing their understanding of the product and potentially influencing their decision to purchase. This part of the question aims to understand whether AR features lead to more informed purchasing decisions, increase buyer confidence, or possibly even lead to higher sales conversions.
- Optimization Through Engineering Management Principles: The second part of the question explores how principles of engineering management can be applied to optimize the integration of AR in online retail settings. This involves looking at the systematic planning, design, and implementation of AR technologies to enhance operational efficiency, improve user experience, and ensure the sustainability of the technology. It considers how engineering management can address challenges such as technological integration, scalability, cost efficiency, and the alignment of AR technology with broader business goals.
The research will likely involve analyzing consumer behavior through data collection methods like surveys or interviews, and examining case studies or existing implementations of AR in luxury retail. It will also include an evaluation of how engineering management principles can be applied to streamline processes, manage technological changes, and enhance the overall effectiveness of AR technologies in engaging consumers and driving sales.
After the Covid-19 pandemic, what do you think are the most serious potential sources of another economic and/or financial crisis that could occur in the future?
In recent years, the scale of the occurrence of serious economic and financial crises in some regions of the world and/or on a global scale has been intensifying. The scale of the appearance of certain types of economic, financial, energy, etc. crises has been increasing since the 1970s. The abolition of gold parity for the U.S. dollar, oil crises, deregulation and liberalization of the functioning of financial markets, increasing the active role of central banks in shaping monetary policies with the possibility of adding money injected into the economy through direct purchase of treasury bonds by the central bank, abolishing some of the previously introduced systemic prudential instruments used in credit risk management, increasing the scale of international operations of investment banks and investment funds making speculative transactions in foreign capital markets are just some of the sources of the increase in the scale and frequency of financial and economic crises since the 1970s. Some types of the aforementioned crises have appeared with increasing frequency and magnitude in the current 21st century. The day on September 15, 2008, when the world's fourth-largest investment bank Lehman Brothers went bankrupt was considered the beginning of the global financial crisis. This crisis was generated by, among other things, erroneously conducted overly lax monetary policies that were supposed to favor financial markets even when assets were overvalued in capital markets, overly liberalized credit policies in mortgage lending, disregarding safety standards in credit risk management and practicing moral gambling in investment banking, and so on. In 2020, there was a pandemic economic crisis, which initially developed through the Covid-19 pandemic to then be exacerbated by large-scale lockdowns imposed in some countries on economic entities operating in selected, certain industries, mainly service sectors of the economy, and the introduction of so-called national quarantines. In some countries, where, such as Poland, the development of the cheapest renewable energy sources was blocked and slowed down in 2022, when the price of fossil fuels rose strongly, a deep energy crisis occurred. Beginning in 2021, inflation began to rise rapidly in many countries, generated by pumping large amounts of printed money into the economies, whose task was to mitigate the scale of the recession generated by the lockdowns and national quarantines introduced repeatedly during the Covid-19 pandemic. In order to limit the growth of inflation, which, as in Poland, rose to double-digit levels, the central bank raised interest rates. The effect of such anti-inflationary measures was to reduce liquidity in the financial sector, increase the cost of borrowed money, make credit more expensive and reduce the scale of investment in many sectors of the economy. The result was a recession of the economy, which in many countries appeared in the 1st half of 2023. In view of the above, the misapplied measures of monetary and/or fiscal policy eased too much led to the generation of a financial and/or economic crisis. Subsequently, the anti-crisis instruments applied more than once led to the generation of another economic crisis. In addition, a climate and environmental crisis is also developing in the long term as a result of continued high greenhouse gas emissions, ignoring issues of protecting the planet's climate, biosphere and biodiversity, slowing down the development of renewable energy sources and implementing the green transformation plan for the economy on a limited scale. In view of the above, some crises like the pandemic economic crisis of 2020, among others, were generated by new factors like the Covid-19 pandemic, which could later be referred to as so-called “black swans” due to their uniqueness, atypicality and unexpected appearance by no one. On the other hand, the key root factors of some economic and financial crises include misguided state interventionism, errors in forecasting and analysis of the macroeconomic situation, misapplied pro-growth and/or anti-crisis instruments within the framework of certain economic, fiscal, budgetary, sectoral and monetary policies pursued by the government and conducted by the central bank. In view of the above, it is probably not possible to conduct economic, monetary, etc. policies without making mistakes. It is not possible to forecast all, future impact factors, determinants shaping the macroeconomic situation and potentially all events that may lead to further economic and/or financial crises in the future. However, it is possible to learn from past mistakes, and given this knowledge, a more sustainable, secure economy can be built, processes and instruments for managing credit risk and other categories of risk can be continuously improved, financial markets, including capital markets, commodity markets, securities markets can be systemically strengthened through prudent use of prudential instruments, not ignoring the principles of financial security, not practicing moral gambling in investment banking, and so on. Perhaps in the future, the next financial and economic crises that will occur will be the result of, on the one hand, still not adequately refined systemic prudential instruments, institutional financial security arrangements, credit risk management instruments, etc., and new factors and events that are difficult to forecast, which can probably later be called the next black swans. however, there are crises that we know will worsen in the future and/or will be the source of the occurrence of increasingly serious negative effects on the economy and humans. this kind of long-term crisis already in operation is the ever-developing and worsening climate crisis and, at the same time, the environmental crisis, which is associated with the process of rapid loss of biodiversity of the planet's natural ecosystems.
The key issues of the impact of the Covid-19 pandemic on the economy and financial markets are described in my article below:
IMPACT OF THE CORONAVIRUS PANDEMIC (COVID-19) ON FINANCIAL MARKETS AND THE ECONOMY
IMPACT OF THE SARS-COV-2 CORONAVIRUS PANDEMIC (COVID-19) ON GLOBALIZATION PROCESSES
The key issues of the problematic sources of Poland's exceptionally deep energy cross in 2022 are described in my co-authored article below:
POLAND'S 2022 ENERGY CRISIS AS A RESULT OF THE WAR IN UKRAINE AND YEARS OF NEGLECT TO CARRY OUT A GREEN TRANSFORMATION OF THE ENERGY SECTOR
Zarzadzanie kryzysowe w przedsiebiorstwie opisałem w artykule:
CRISES IN THE ENVIRONMENT OF BUSINESS ENTITIES AND CRISIS MANAGEMENT
I described the key issues of opportunities and threats to the development of artificial intelligence technology in my article below:
Anti-crisis state intervention and created in media images of global financial crisis
In view of the above, I address the following question to the esteemed community of scientists and researchers:
After the Covid-19 pandemic, what do you think are the most serious potential sources of another economic and/or financial crisis that could occur in the future?
What do you think are the most serious potential sources of another economic and/or financial crisis that could occur in the future?
What do you think about this topic?
What is your opinion on this issue?
Please answer,
I invite everyone to join the discussion,
Thank you very much,
Best wishes,
Dariusz Prokopowicz
The above text is entirely my own work written by me on the basis of my research.
In writing this text, I did not use other sources or automatic text generation systems.
Copyright by Dariusz Prokopowicz

I am looking into purchasing an automated cell counter. I have used the Countess previously, but was wondering if there are other recommendations for counters that are reliable? Are there any good cell counters that do not use disposable slides?
I'm gonna to make arthritis model induce by serum from K/BxN. However, K/BxN mice are unable to breed. So where can I purchase K/BxN serum?
I am planning to work on different water bodies of Andhra Pradesh. So I want to purchase a portable water testing kit with multiple parameters. Please suggest me a few best option, which would be a great help for me.
I am looking for a compound called Tryptoquivaline F/J (or Fumitremorgin F/J) for some validation assays. Either to purchase or synthesize. Thanks!
For my reseach, I had purchased several frozen vials of HFSC(Hair follicle stem cell) from Celprogen, but I could not characteriz the Celprogen-HFSC with several anti-human antibodies for recognizing human HFSC. In addition, their mophology look like epithelial cell with round shape, and was growing very very fast like cancer cells. I did whole RNA sequencing of Celprogen-HFSC, and the data for Celprogen-HFSC indicated the cells were not definatly from human sources, perhaps was CHO cell. I claimed the Celprogen several times but they always answered there was no problem in managing cells in Celprogen. At that time, I did not want to waste my time to conflict with Celprogen any more. However, recently, one of my companion purchased a frozen vial of PDLSC (Periodontal ligament Stem cell) from Celprogen, but also he had the same experience as I had. The cell morphology is not mesenchymal lineage, showed epithelial like appearance with bursting proliferation activity. Hence, I asked anyone who experience such things after purchased from CelProgen. If yes, please answer here or send me email: yshwang@khu.ac.kr.
I am a postgraduate research student working on cancer biology in India. I need Danio rerio (Zebrafish) for this study of mine at the earliest. Can someone please provide me with contacts for places where I can purchase zebrafish in India and expect a quick delivery?
I appreciate any help. Thank you so much for your attention and participation.
Please provide information on where I can purchase a 150 kDa, 500 kDa, 1000 KDa and 5000 KDa molecular weight cutoff membrane? for separation of ferulic acid (194 mwt) form mixtue of sample. where almost other molecular wt cut of is ranginng form 100 - 150.
I have strated lateral flow assay and the components are from clonegene. results are faint not clear...sometimes test line is coming sometimes control line is coming ..what should be the reason? Is there any paper availavle from LFA who have used clonegene components for my reference?


There are two interference peaks in 1X PBS by DPV methods in three-electrode system which locate in 0.274V and 0.517v.
PBS was purchased from J&K Scientific Ltd which was blowed by nitrogen for 3 minutes before test in order to remove oxygen . And my working electrode was gold electrode made by sputtering. Ag/AgCl electrode was used as reference electrode.
Is there anyone could help me explain this phenomenon?
Dear Forum Members,
I hope this message finds you well. I am currently considering the purchase of a Q-TOF LCMS-9030 Mass Spectrometer for my laboratory, and I would greatly appreciate hearing from anyone who has experience working with this instrument.
If you have used the Q-TOF LCMS-9030, I would be interested in hearing your thoughts and experiences. Specifically, I am seeking reviews on the instrument's performance, reliability, user-friendliness, and any limitations or challenges you may have encountered.
Your insights will be incredibly valuable in helping me make an informed decision about this purchase. Thank you in advance for sharing your expertise and feedback.
Best regards.
Has anyone purchased or used the table top BD Facs melody cell sorter? Our lab purchased one 1.5 years ago and it has been the most frustrating machine ever. The stage locks up, techs have to come out every 2-3 months to fix something and reboot the software, etc. I am trying to ascertain other researchers experiences , if any, with this model. I believe we have an absolute lemon but I want your feedback.
This year we have the opportunity to purchase an HRMSD. We have an Agilent Infinity II 1260 HPLC in our lab and are planning to purchase a Q-TOF. I have experience with Agilent equipment (GC/LC/GC-MS SQ/LC-MS SQ), but not with HRMS.
We are planning to use this instrument for untargeted metabolomics. We are interested in searching for producers of new antimicrobial compounds, performing screening and identification of new antimicrobials of microbiological origin, and studying the biosynthesis of microorganisms.
So far, we have been offered two Agilent detectors:
1. 6546
2. Revident.
As I know HRMS produces huge amounts of data, and performing untargeted metabolomics workflow requires additional software to work with the data in case of untargeted analysis.
Now Agilent offers two sets of software, which is a bit confusing for me.
1. Let's call the first set "classic". It includes:
a. MassHunter Profinder (for Feature finding stage)
b. Mass Profiler Professional (MPP) (for Alignment and statistics)
i. ID Browser (module of MPP for Identification)
ii. PathWay Architect (optional MPP module for metabolite pathways buildings)
c. METLIN PCDL for LC/Q-TOF (database for metabolomics)
2. Let's call the second set "new" It includes:
a. software product - MassHunter Explorer, which, according to the manufacturer, combines all of the above software products into one.
b. ChemVista library manager with METLIN PCDL library
Questions:
1. Is the MassHunter Profinder standalone SW or is it part of MassHunter Qual or Mass Profiler Professional
2. Which one of the SW sets should be chosen? They do the same. But do they really do the same and have the same capability? Marketing? From my experience the early version of SW is quite restricted. For 6546 and the newest Revident Aglent recommends MassHunter Explorer.
3. To buy or not to buy:
a. I read that untargeted analysis has a huge community and freeware databases and SW for metabolite identification. Is METLIN PCDL library a MUST part of SW from the Vendor? Can I consider it as optional and use freeware DB?
b. The same question about ChemVista library manager?
4. By which SW/Databases do you realize your untargeted metabolomics workflow?
5. Any experience with the Revident model of Q-TOF. How far is it better/worth in metabolomics compared to 6546? Marketing?
a. Intelligent functions
b. Solutions for Tuning
6. Is the APCI source essential for untargeted metabolomics?
Thank you in advance.

i want to know your thoughts on this topic and how i should tailor mt research.
I am looking for a small (approx. 1 cm2) wireless (preferably Bluetooth) potentiostat/potentiometer for measurements in closed wounds, e.g., implantable potentiostat. At first, I am interested in the simplest potential measurements (range from - 400 mV to 200 mV, measurements for 1-2 weeks in implanted situation). Let me know if you have such or know where to purchase.
We are trying to dry trace amount of water from anhydrous acetone we purchased because we found that it still reacted with our moisture sensitive samples. Thanks.
I noticed that many researchers compared electrocatalysts for hydrogen evolution and oxygen evolution reactions with the activity of 20%Pt/C and RuO2, respectively. Could you please tell me how to prepare those electrodes or recommend a suitable supplier for purchase? When I visited the Sigma Aldrich website, they only had 10% Pt/C electrodes. I would greatly appreciate it if you could respond or offer advice in this regard.
Thank you
I am looking for tools to measure the mindfulness and wellness among health care workers. It seems that FFMQ or FFMQ15 are commonly used to measure mindfulness.
Questions:
- Is there a purchased license required for use the FFMQ tool(s)?
- Anyone used the FFMQ or FFMQ15 previously? It seems that internal consistency and sensitivity to change are adequate for both versions. What was your experience with data analysis?
Gu J, Strauss C, Crane C, Barnhofer T, Karl A, Cavanagh K, et al. Examining the factor structure of the 39-item and 15-item versions of the Five Facet Mindfulness Questionnaire before and after mindfulness-based cognitive therapy for people with recurrent depression. Psychol Assess 2016;28(7):791-802.
Kind regards,
Grace
the research objective is "Identify the key factors of brand equity that influence the decision-making process of young female customers when selecting a clothing store to purchase clothing items."
And also, I have a question, that how do the price, quality and style impact to the brand equity of clothing stores.
I am looking for a mammalian cell media with a few specific supplements, but it is not available for purchase from any vendor. The lab-grade supplements are pretty expensive and are sold in either huge tubs or in tiny amounts. I thought to just buy the required supplements (L-arginine and L-lysine) in the pharmacy and add appropriate amount to my media. Do you think it is ok? I shouldn't list the source of supplements when it is time to publish? The pack size and purity of pharmacy-sold supplements seem to be suitable for my experiment.
Cheers,
Maria

Just anybody who has an interest in generic marketing issues
I am searching for antibody to rat brain tissue. I would prefer to purchase antibody for both Western blot and Immunohistochemistry.
Thank you in advance.
The purchased EVA (VA content 40%) product is dissolved in toluene solution, and then a mixture of sodium hydroxide and alcohol is added, the concentration is about 1mol/l and 0.25mol/l. The infrared test results of the product are normal, but it has been unable to dissolve in DMSO. There is no major research in this area can answer or discuss. Whether the EVA synthesized by oneself and then make EVOH will dissolve in dmso better.
Hi all,
Does anyone know where I can purchase a TEM sample rod holder that works with FEI/Thermo Scientific TEM rods and allows for 360° rotation to check and grease the O rings?
I have an example image attached. I shows a sample rod holder that Fischione provided with our tomography holder. This one doesn't work for double tilt holders, however.
I don't know why it's so hard to find this basic but fundamental accessory. JEOL seems to provide these with their holders. I don't know why FEI/Thermo doesn't, but it's causing all kinds of vacuum troubles not being able to easily check and grease the O rings. Thanks!
Sheri
Hello all. We are trying to get access to prostate cancer cell lines that were derived from patients who were Black or of African descent. There are numerous relevant cell lines reported in the literature, but they are hard to find for purchase - the only one we have found for sale is MDA PCa 2b / CRL-2422. Would anyone be able to provide a link to any others that are for purchase, or else be willing to share a flask or vial with us if you have some in your lab? We have reached out to some authors who developed relevant cell lines, but unfortunately haven't heard back yet.
Many thanks!
I recently purchased a vial of Cynom-K1 cells. I grew them up over several weeks as they were of VERY poor viability when they showed up. Expanded them, froze back 35 vials, saved a couple million and did a DNA extraction. Amplified an intron from a region for which I had Cyno and Rhesus sequence to confirm that these were actually Cyno cells. To my surprise they were HUMAN. A warning to anyone getting in cells, make sure you know what you have before starting any work with them.
Hi All,
I'm wondering if anyone knows where I could purchase a physical copy of the "Journal of Comparative Neurology: Research in Systems Neuroscience, Volume 524, No. 16 (November, 2016)". Picture attached.
Thank you for your help with this!
Kind Regards,
Ariel

Could you please assist me in finding survey questions related to influencer marketing in Uzbekistan? I'm particularly interested in understanding consumer perceptions and behaviors influenced by influencer characteristics, cultural factors, and the competitive landscape. It would be helpful to have questions that explore the impact of influencers' attractiveness, credibility, trustworthiness, and exposure on consumer brand perception and purchase intentions. Additionally, questions that address the challenges faced by marketers in this context, such as cultural nuances and navigating the competitive digital marketing industry, would be valuable. Thank you for your assistance in providing relevant survey questions for this topic.
Explanation:
Only 57% of the expenses for urban trains and 40% for suburban trains have been recuperated, a fact that regular train travelers might have observed through ticket prices.
Despite these challenges, Indian Railways has achieved a remarkable milestone in the 2022-23 fiscal year, reporting a record revenue of ₹2.40 lakh crore. This represents a substantial increase of almost ₹49,000 crore compared to the previous year, as highlighted in a ministry statement on Monday. Notably, the freight revenue experienced a significant uptick, reaching ₹1.62 lakh crore, marking a robust growth of nearly 15% from the preceding year, as reported by NDTV on April 18, 2023.
Does anyone know where I could purchase gold-core silver-shell nanoparticles in a dichloromethane solvent? Our usual supplier only seems to have them in water.
Hi
I'm working on cell imaging by Raman mapping.
Recently, I purchased the water immersion lens and measured the cells in the PBS.
When I use water immersion lens I often get some lines in the result after processing data, which did not appear before.
What I thought the problem is the water immersion lens but I can't sure about this.
Is there any opinion can help me?

I am currently a senior high school student, and I consider myself a beginner in this field. We are tasked to conduct a qualitative study about the effects of influencer marketing on purchase behavior. I am interested to know and hear what theories or theoretical literature that can be applied in our research.
Thank you in advance!
I have a case in my field research
I want to know the consistency of the fish troops at Market X
I recorded data for 7 days (from August 11 to August 17) regarding:
(A) Fish sold by traders at Market X
(B) Fish purchased by consumers at Market X
The respondents I interviewed were 30 traders at Market X
I conducted a census of the same 30 traders for 7 days (from August 11 to August 17) regarding:
(A) Fish sold by traders at Market X
(B) Fish purchased by consumers at Market X
What analysis is suitable to be used to provide an overview of the consistency of fish supply in Market
A phenomenon that is clearly visible from the census results is that the types/variations of fish purchased by consumers are less than the supply of types/variations of fish sold by traders.
Do any of you have a case like mine?
We are currently conducting a qualitative research on the effects of influencer marketing on purchase behavior. However, before that, our panel has suggested to conduct a pre-survey to (1) identify the products endorsed by influencer marketing that students mostly purchase, and (2)the social media platforms that students purchase influencer-endorsed products. The purpose of the pre-survey will help us narrow down our scope based on the results of the pre-survey by focusing on a specific social media platform and product.
Our question is how can we determine the sample size?
Thank you in advance!
Hi, i am looking for a survey questionnaire which involves the marketing mix strategy and purchase decision? Help will be appreciated if anyone came across this question on researchgate. THank you very much!
I am currently a senior high school student, and I consider myself a beginner in this field. We are tasked to conduct a qualitative study about the effects of influencer marketing on purchase behavior. I am interested to know about purchase behavior for our research project in marketing. Is there any literature that may help? May I ask what is purchase behavior and how does it differ from consumer behavior?
Thank you in advance!
I am trying to analyse the polyphenolic or flavonoid content of my honey sample. Can I do it with some new method that doesn't require purchasing any standard for chemical compounds?
Hi everyone.
I'm in the process of purchasing an stereo-microscope of the Amscope brand, specifically an SM-2T, but I ran into this thought of what if this microscope is not suitable for what I'm looking for?. I searched for academic papers and, indeed, some of them report using this brand but different models and magnification range. The equipment I'm purchasing has 7x-90x magnification range, a 2x magnification barlow lens, and a 5MP camera attached. So... does anybody has this microscope and work with microplastics by any chance that could lead me in my purchase or has any experience in observing very tiny microplastic-like objects with this equipment? It'd very helpful for me to avoid making a bad acquirement.
Thanks for your time and attention.
Hello everyone,
I would like to purchase male microneedles. Would you please introduce me a company which make them?
Thanks.
Bunker fuel suppliers around the world have the tendency of delivering less than what they state in their Bunker delivery not (BDN), leading to commercial losses for the purchasers
We can purchase poly-lysine coated coverslip but they have an expire date. Could we coat again with poly-lysine some expired coverslips?
Please suggest diet analysis software for 24-hour dietary recall and the price of purchasing the software.
I am looking for guidance on stratified sample sizes. I would like to analyse buying behaviour of businesses within a total population of 100,000.
There are 12 different types of businesses within the population. Each have different behaviours when it comes to what they buy, what volume they purchase, and how frequently.
I want to survey these businesses to analyse their different behaviour.
I know the total population, and the population of each business type.
I want to achieve high accuracy, but within a budget.
So my question is: what is the sample size?
Is it the statistically correct sample size for the total population, stratified by the business types?
Or is it the statistically correct sample size for each business type, added together?
Dear All,
I did the extension experiment with 20mer RNA primer annealing with 40mer RNA template in the presence of recombinant human coronavirus SARS-CoV-2 RNA-dependent RNA polymerase (RDRP) which was purchased from the vendor. No extension product was observed but some products which were lower than primer shown were found (please see the attached screenshot). Does anyone know the possible reason to cause this? The primer and template were purchased directly from IDT and they are still good (I checked the purity via HPLC). And the polymerase was purchased in July and are stored under -80C until using. Thanks in advance!

Having a bit of trouble with working out some calculation's.
I have purchased 1mg PMA and plan to dissolve in 1ml DMSO to get a concentration of 1mg/ml.
My plan is to aliquot thESE and freeze. Perhaps 1ul in each vial?
However i am unsure of how many mls of media to add to each aliquot to give the final concentrations of 2.5ng/ml, 5ng/ml, 10ng/ml, 20ng/ml and 50ng/ml?
Probably quite a simple calculation but any help would be greatly appreciated!
Thank YOU
Hi!
I am trying to purchase the protease MMP21 but wasn't able to find a commercial source. Does anyone know of a distributor? I am located in the US.
Thank you!
Nadine
Please how much will it cost me to purchase primers for infectious laryngotracheitis? and where can i purchase these primers?
I used to work with a PM-IRRAS + Langmuir balance from KSV NIMA but it is no longer available. I want to purchase a PM-IRRAS module compatible with Langmuir balance, does anyone know another company or have experience with other similar setup?
I am looking for large data sets of medical imaging, mostly related to neurology. Specifically, a wide array of CT, MRI, diffusion, CTA, PE, and the like. I was wondering if anyone can share some companies they may be aware of that sell such datasets?
Unethical business practices operate in different countries to varying degrees and participate in economic processes.
For many entities, market participants, business partners and consumers, they generate additional costs.
They can also be a source of gray economy growth, including avoiding paying taxes.
Thus, there are social costs for individual entities and financial for the entire economy.
On the capital markets, one of unethical business practices is, for example, insider trading, ie the use of confidential information by decision-makers with access to confidential information used to conduct transactions to purchase or sell financial instruments, including securities or other securities or other capital markets.
In individual countries, there are various instruments to combat the use of unethical business practices, the shadow economy, etc.
The effectiveness of individual normative solutions, the scale of restrictions applied, and the business mentality of market participants, entrepreneurs and businessmen are different.
Another mentality is related to the level of awareness regarding corporate social responsibility.
In individual countries, social campaigns are carried out suggesting the legitimacy of developing concepts based on corporate social responsibility.
In view of the above, please answer the following question: Is unethical business practices a negative external effect of non-ideal market structures or imperfection of the social market economy?
Please, answer, comments. I invite you to the discussion.