Pulmonary - Science topic

I would like to participate in publishing systematic reviews and meta-anlysis in my research field (public health) and longing to join research teams working with this kind of methods. I would appreciate any advice or guidance. Thanks

Hi !

i m doing research and wish to know if their is stewardship In TB?

i understand that their is stewardship in extra pulmonary Tb and Drug resistant TB only !

I'm looking for some lineage of human pulmonary endothelial cells that isn't from patients with adenocarcinoma. May you help me by suggesting any?

Thanks!!

Hi all,

I'm growing primary basal cells from lung digests, and was wondering if anyone has recommendations for cell freezing media for primary lung basal cells.

Previously I have been using Cryostor CS10, however I'm finding quite costly and would like to make my own.

My expansion media is Pneumacult EX Plus.

Many thanks,

Sam

I am working on a rare case of mesothelioma and I am interested in knowing the epidemiology, especially in African countries

Need advice: Patient 37 y.o. with DVT and thrombosis of right radial artery (after coronary angio 2 weeks ago), pulmonary embolism and thrombolysis month ago. Now patient has indication for on-pump cardiac surgery - resection of postinfarction aneurysm of LV with thrombectomy (NB! Floating thrombus in the LV). Patient receives anticoagulants. Mild form of COVID-19 - 3 weeks ago.

What tactic is better to choose: operate now or wait 3-6 months after pulmonary embolism?

Thank you in advance!

I recently treated mice with clodronate liposomes but there seems to be no significant depletion of macrophages in the lungs. what could be the cause

I've read many posts and articles touting the use of externally applied low dose radiation to the lungs to address the inflammation brought on by COVID.

Since, as pointed out in our paper on the possibility of a direct anti-viral impact from xenon-133, nuclear medicine lung ventilation has the unique ability to bring ionizing radiation to the entire respiratory system, shouldn't it be explored as a means to provide a more targeted low dose therapy than available from an external beam approach?

Lungs MRI images are needed for detecting CIVID -19.

We need to quantify the lung 🫁 damage caused by chronic silicosis among silica dust exposed workers in various occupational set up. We need some realistic idea including novel ideas for approximate estimation. Seeking your help.

A project in my lab involves single cell RNA-seq data analysis of mouse whole lung samples. However, when we analyzed and clustered the dataset and searched for markers to annotate the group, there appears to be few to no cells exhibiting the classical epithelial cell markers EPCAM and CDH1.

Each sample has around 8000 cells after filtering for mitochondrial content, and the overall quality seems fine. But over half of the cells appear to be immune cells and there were less than 100 epithelial cells for each sample.

The mouse models are established by a collaborating group, and whole lung samples are sent to a sequencing company (travel time about 3 hours minimum) to generate the scRNA data. Our collaborators have adjusted experimental protocols multiple times to increase cell viability (~85%), but we are having difficulty fixing this lack of epithelial cells.

Does anyone have some experience with this, or know why there would be so few epithelial cells in scRNA-seq data of mouse lung samples?

Both my lab and our collaborators are fairly new to handling scRNA-seq data, so any insight would be helpful.

Coronavirus disease (COVID-19) pandemic is a global health problem. Infected patients usually have respiratory symptoms due to lung involvements. However, liver impairments could be another findings.

So does Covid-19 affect liver functions & how?

A 65 years old male patient, once a heavy smoker and HBV carrier suffers from some form of cancer with pericardial sac metastasis.The PET/CT shows there are four nodules with high SUVmax (higher then 2.5 ).One of them locates in the lower lob of right lung，16.2×10.9 mm in size，with SUVmax=3.5.The rest three nodules, adjacent to mediastina, are bigger in size(21.6×20.6 mm，33.4×18.8mm and 30.2×30.0 respecttively) and higher in SUVmax（ 8.7，5.8 and 7.1 respectively).All the other organs in the abdominal cavity are normal,except for liver.There is a nodule，24.3×18.2mm in size，with SUVmax=1.6. The IHC result of the cells collected from pericardial effusion shows：TTF-1(-)，Naspin-A(-)，CK5/6(-)，CD56(-)，Ki67(5% +)，CK7(+)，CK20(-)，P40(-)，Calretinin(-)，WT1(-)，PAX8(-)，TTF-1(-)，CDX2-88(-)，GATA3(-)，NKX3.1(-)，Claudin4(mostly+)，MOC31(-)，Syn(-).The question is what kind of cancer is it,a adenoma, squamous cell cancer or neuroendocrine tumors?The other question is the histological origin of these metastases, the lung or the liver?Can any one give me some advice？Thank you！

Hi everyone.

I want to establish Precision-cut lung slices (PCLS) by using peripheral lung, for example about a 3-cm specimen.

Can I get PCLS by injection 1.5% low melting agarose to the lungs?

Thank you!

I am trying to check the effeciency of my segmentation results via CT images. I am looking for a publicly available and annotated dataset of ct images for lung segmetnation. I would be great if the iamges in the dataset were in easy format such as JPEG.

Are there any seggestions?

Thank you in advance,

Because as we know a smoker has less healthy lung than someone who doesnt

Hi,

Im trying to isolate intratestinal macrophages from mouse lungs, and grow them in culture for a few days, to collect secretome. Does somebody have a good protocol for it?

I tried face sorting but the cells didn't grow well and died in the following days.

I also tried to separate f4/80 cells with macs columns but didn't get any separation.

Can someone have any suggestions?

Thank you,

Shani

Please suggest some nebulizer/inhaler which specifically administer the nanoparticles into the rat/mice lungs.

Is there any company makes such device for rat/mice ?

Hi,

We are doing focused scSeq of lung cancer and we need a panel of genes to identify and differentiate the lung epithelial cells from other cell types of lung. What genes would be useful?

Best regards,

Morten

Some of the Authors considered either sinusoidal function or breathing waveform. Anybody can suggest the exact, realistic breathing condition for the inhalation and exhalation to simulate particle deposition in a human lung airway?

In COVID 19 detection if we will separate out clevicals and ribs in lung images.Weather we can improve accuracy?

Hello-

I have been doing BAL fluid collections on mice, with the intention to extract RNA from the cell pellet. I know there are many ways to do this, but the optimal method I landed on is to use 3mls of PBS, one at a time. I've been lavaging/washing each ml 3 times before finally aspirating it and collecting it in a tube. With this method, I was able to get a big enough pellet to get sufficient RNA from a single naive mouse, and have been able to consistently replicate that.

The next step was doing this on mice that were treated with something that causes lung inflammation. I've tried it twice and both time, the RNA yield was significantly lower. I did notice that more of the PBS ended up in the thoracic cavity, so I imagine that's where the cells went and why I was unable to get more RNA.

So, my questions are: is it likely that I didn't do the lavage gently enough, for damaged lungs? Should I limit each ml to one or two washes, or use smaller amounts at a time? Do I need to dispense and aspirate the PBS more slowly? How can I maximize my cell yield in this situation?

Thank you-

Amy

The characterization of heavy metals like gadolinium in organs like liver, brain, lungs can best and simply be done by.....................

Hi, I need to transfect mice lung fibroblasts with lenti-cre. I bought one from a company but it didn't work at all, efficiency's super low. Can anyone recommend the premade lentil-cre particles that actually work? I'm in US. Many thanks!

What Is The Relationship Between Vibration Of The Chest Wall And The Resulting Chest Wall Forces, Chest Wall Circumference, Intrapleural Pressure, And Expiratory Flow Rate?

Although most people with COVID-19 have mild to moderate symptoms, the disease can cause severe medical complications and lead to death in some people. Older adults or people with existing chronic medical conditions are at greater risk of becoming seriously ill with COVID-19.

Complications can include:

Hello,

I am running airflow simulation through a model of lungs. I want to find the velocities and pressures at certain locations. I tried creating ISO-surfaces but this surface cuts through the whole geometry and I want only specific locations on the geometry as shown by the colored lines in the figure. Is there a way to create a plane at the location of these lines exactly that will give only this branch? I want to find the velocities and pressures at the end of each branch.

Thank you in advance

Hi all-

In our lab we work with human lung biopsies in which we digest and plate on fibroblasts to produce beautiful clones.

After the clone shave grown for 7-14 days we trypsinize and resuspend in StemCell Cryostor.

We have never had a problem when thawing and re-plating, but recently we haven't been able to regrow the cells after thawing to the full extent as the could be.

Taking any and all suggestions if you have run into this problem or if you have a protocol for freezing down and thawing lung epithelial cells that works wonderful for re-plating.

Thanks

Because surgery methods used during tumor resection induce a blood accumulation in non lesional lung that we recovered, we wondered if someone know how to eliminate blood before lung digestion ? In order to avoid a contamination of tissu resident cells by circulating cells which can change the frequency of cells analyzed by flow cytometry ?

I look forward to reading your answers

Dear fellows,

I am looking for an expert in lung histology to collaborate on one of my project.

The expected work is to analyze histology images (e.g H&E, Trichrome, IHC etc) I have obtained from lung tissue from different mice models and provide me with an accurate description, quantification, an proper figure panels of whatever phenotype he/she will observe. The researcher will of analyze all the samples blindly.

For this work the chosen collaborator will of course be one of the co-authors of the future publication using this data.

look forward to here from you.

Best,

Yair

Hi,

I am currently looking into Trichrome stained section of mouse lung and trying to understand different parts of the lung but it is a bit confusing.

As far as I understood 1,2 are bronchiole, 3-pulmonary artery, 4- not sure, 5-also bronchiole?, 6-artery, 7- bronchiole as well?

Major confusion is between 1 2, 5 and 7 which all look like bronchiole but with different histology, and 4 which I can't clarify.

If anyone can distinguish these please let me know. Thank you so much in advance!

For a depot injection designed for absorption by macrophage action, which injection route would be optimal to have most drug introduced into the lung by the lymph vessel?

I am working on a project to classify lung CT images using CNN, and

as I understand, the pixel spacing is distance between the center of each pixel, specified by a numeric pair - adjacent row spacing and adjacent column spacing in mm (this is in 2D space), but because the CT images are 3D, the pixel spacing shows a triple value, and I thought that the second and third value are row pixel spacing and column pixel spacing, but the first value is Z dimension which means slice thickness, I am right or not?

the another question is: as it's known, CT scans has a different slice thickness, if I work on 2D slice of CT, I need to make all slice the same thickness or its only necessary when working on 3D scans?

Research coming out now supports the idea that initial Viral Load has an effect on the eventual outcome of disease. A person exposed to a lower initial number of COVID-19 Viruses, has a better chance of their immune system being able to eliminate the Virus while it is still present in the Nasal Passages and Throat, as opposed to having the Virus spread to the lower respiratory tract and then begin causing lung involvement. For many it appears small numbers of the Virus manage to spread all along the upper and lower respiratory tract, and cause a full myriad of symptoms but the disease ends up remaining mild.

Hello !

We have one patient who presented pulmonary embolism and haemmorhagic stroke in same time.

So, anyone can help us for the management of anticoagulation ?

We see reduction in PA pressure after mitral commissurotomy and MVR

Does it persist in certain subset of patients?

We have many cases with normal D-dimer with the normal CT pulmonary angiography.

We are going to do mouse silica-induced lung fibrosis model. However, in the scientific papers the exact purchase details of e.g. DQ12, D50=2.2 um silica is missing. How dis you managed it when you did such models? I would appreciate some details. Thanks

Hi,

In my last experiment, I lysed RBC in a whole lung pellet with ACK. Even when I leave it for longer than 1 minute/ sample (protocol in my lab), the pellet is usually quite white. This time I noticed something grey at the bottom. Then I counted the WBC towards the end for my different groups I noticed there was a lot of debris. I'm not sure if it's the ACK or other enzymes in the lung digestion or the treatment I provided to the mice (I have never seen this until now). Any suggestions/guidance would be appreciated

Please recommend buffer to homogenize lungs if I want to study phosphorylation of NfKb, STAT and IRF protein by Western Blot.

Trying to segment X-ray images of lung using MATLAB. I want to segment all the images automatically, but with my code it can be applied only on single image. Getting issue with seed selection.

Post covid Lung fibrosis is hot topic now a days. I found most of the post covid shadow resolve without any medication. I hardly see actual case of Lung fibrosis specially after 12 weeks of discharge

Ivermectin well-known drug - gave promising results in the treatment of Covid 19, although there is a concern, based on the fact that the concentration of free drug in the blood ( with safe doses ) is low compared to its in vitro IC50 against SARS-COV-2

My hypothesis is that some metabolites may be effective against the virus and accumulate inside the lung cells to reach the effective concentration. Is there any study that confirms or deny this hypothesis?

I have rat stomach samples that were confirmed to have adenocarcinoma. Similarly this was found in the lungs. How do i confirm that the adenocarcinoma in the lungs came from the stomach? Is mucin IHC staining suitable for this? Thanks

Hi everyone,

I am looking for a public data set on RNAseq of human lung adenocarcinoma. I am looking at composition of microenvironment and particularly interested in DCs subsets within the tumour. I have never dealt with big data before and I have no idea if there is a public repository of this kind of data. Hope you can give me some insights!

kind regards,

Rita.

I am looking for documented scientific articles about the role of physical therapy Pre and Post major surgeries.

We read that people who breathe a lot of dust or asbestos can suffer from fibrosis in their lungs. I have seen that some people also suffer COPD without that and wondered if it has ever been related to high cholesterol? ie. Can fibrosis of the lungs be created from multiple tiny pulmonary embolisms over time when someone has high cholesterol?

Genetic profiling reveals an alarming rate of cross-contamination among human cell lines used in China and SPCA1 is one of these cell lines.

As the Expasy shows "Problematic cell line: Contaminated. Shown to be a HeLa derivative".Would it be ok if I continued to use SPCA for lung adenocarcinoma research?

Dear Colleagues,

I try to isolate Mycoplasma sp. from lung samples in rabbits but it is really difficult. Do you have any experience about that? Mycoplasma pulmonis was found just in mice but not in rabbits. Could you tell me what kind of spieces could I find? What kind of medium should I use?

Thanks in advance.

Silvia

Hi,

I'm facing 2 issues with our LLC and B16/F10 melanoma model in order to count lung metastasis.

In order to get significant metastasis, we need to incubate LLC and B16/F10 in mice for about a month. (LLC administration iv (tail vein): 5x10^5 cells/mouse / B16-F10: 1x10^6 cells/mouse IV).

At that endpoint, we obtain huge variation from mouse to mouse within the same experimental group.

How do you reduce this variation inside a same experimental group?

Is there another reliable cancer model to adresse metastasis in the lungs aside LLC/B16-F10 or urethane-induced?

thanks in advance

Hi all,

I plan to do IHC to distinguish monocyte-derived macrophages from interstitial and alveolar macrophages in the lung in non-human primates, but I'm having a hard time finding a good marker. It seems that I can distinguish alveolar vs interstitial macrophages with CD206 and CD163, then maybe use CD14 for monocyte-derived macrophages, however, I'm seeing literature describing the loss of CD14 and gain of CD206 in monocyte-derived macrophages that have remained in the lung and begin to undergo "polarization" as replacement alveolar macrophages after infection. Anyone have suggestions or suggested reading?

I would like to culture the lung fibroblasts with TGF-b stimulation, but i don't know how to make sure the cell density, and i also have no idea about what kinds of plate (e.g. 6 wells plate? 12 wells?) or flask i could use to culture the cells with tgfb stimulation. Could you guys please give me some clues?

I am conducting a program evaluation plan for an outpatient pulmonary rehab program and am having trouble determining the statistical analysis methods to use. The following process and outcome evaluation questions I have are:

Any help would be appreciated in determining the appropriate statistical test to use as well as the phrasing of questions. Thank you!

Are there colleagues who would like to work on ground-glass opacity ("verre dépoli") evaluation using computed tomography (CT) imaging of the lungs ? The work can be extended to crazy paving (combining frosted glass, inter-lobular and intra-lobular cross-linking) evaluation. More details can be given later.

I used several thresholding methods that were not helpful because they also remove the parts that are important for corona detection.

for example:

Thank you for introducing a simple and practical method for lung extraction from CT scan.

Dear all,

I would like to measure the total lung SUV on Horos. Is it possible to segment lungs on CT and warp the segmented volume on the PET images to measure the total lung SUV?

I'm currently working on a lung infection model for a project, and have tried EVERY method we can find for consistent intratracheal administration of our infection (biolite, small tubing down the trachea, catheters, feeding needles, you name it we've researched it.) It seems like the Penn Century microspray device is the top of the line in a well distributed and consistent infection and/or treatment, and we really want to use one.

Unfortunately we cant find one, anywhere. They went out of business in 2015. If your lab has one you rarely use, we'd love to buy it. We'd prefer not to purchase the $2000 one from Bio-Equip in China, they aren't licensed by penn century and we don't know what quality we would get (if you've ordered this other version, please tell me about it!)

Help?

We do mouse lung imaging and inhomogeneous instillations are clearly visible.

Thus we are looking for a substitute for the Penn-century MicroSprayer® Aerosolizer because this company went out of business.

Any clue?

Thank you

Philippe

Many suspected cases of Corona virus are self or home quarantine , some of them may have difficulty in breathing and other symptoms but are still having negative report for coronavirus disease. So what should be the advice given by Physiotherapist to them.

I'm having trouble with a nuclear protein immunostain of cells (lung fibroblasts) cultured on soft (1 kPa) collagen-coated hydrogels. Can anyone give me a suggestion?

Hey,

I am currently investigating acute respiratory distress syndrome (ARDS) in mice via intra-tracheal infection of mouse lungs with E.coli. Thus far I have validated our model using histological approaches. However, I would also like to have some real time monitoring to assess the progression of the infection/ARDS symptoms. I have been doing peripheral blood oxygen measurements. However the SpO2 % measurements can have high variability across my experiment. the pulse oximeter we use takes quite a long time to read a stable measurement, sometimes I can be stuck measuring a single mouse for 20 minutes. Often, I get two stable readings from my mice. For example, control uninfected mice ideally would have 99% SpO2 saturation. however I can also get a clean stable reading at ~85%. For my infected mice, the values vary. Since there is no standard, its quite tricky to determine which measurement is true. For example, an infected mouse, 6 hours post infection, can have a reading of 77% SpO2 and can then immediately read stably, at 50%.

I am aware of ensuring the mouse is accustomed to being restrained and recovers from that stress before measure blood oxygen. In addition, extremely low stable readings (60%<) can suggest a mouse may need to be euthanized, but qualitative assessment of the mice, exhibits they are are fine and very active, so it makes me doubt extremely low but stable readings.

Has anyone had issues with getting accurate SpO2 readings with mice and have any tips for accurate measurement and interpretation?

I did Immunofluorescence staining of DAPI using the lung tumor tissue frozen section which contains part of the normal site, but I find it hard to figure out why the DAPI staining (or other antibodies) intensity was weaker compared to the normal sites under the same condition. I repeated it more than once, I don't know what's wrong with it.

I know in the literature all papers that describe syllable structures in Arabic, referred to the following five patterns:

CV, CVV, CVC, CVVC, CVCC, and in some dialects CCV(V)C(C).

However, all these papers considered VV as the longe vowel, NOT two different vowels in a sequence!!

Therefore, could we consider Arabic as a language with only three core syllabic structures: CV, CVC, CVCC, where the CC is only a true consonant cluster with two different consonants, and V is the vowel either long or short???

Hello,

Searching for the ultimate housekeeping gene (with expression stability) that works well in human pulmonary fibroblasts (besides GAPDH), any suggestions?

Hi all,

I have been experimenting with human lung fibroblasts and the effect of nintedanib and pirfenidone upon them in both normoxic and hypoxic (1% O2) conditions. I want to see the effects on ABCB1 expression therefore I am blotting for this protein. However, I see a faint band at the 140kDa range (correct band according to literature) but another stronger band just above 100kDa which I am struggling to account for. The literature suggests glycosylated protein may accumulate as a smear but this is seen at a higher range (180-300kDa). Some of the images from the websites selling the antibody show the band I am seeing, but none explain its presence.

If anyone has had any similar experience and could help me to identify this extra band it would be much appreciated.

Best wishes

Dr. Robert J. Lawrence

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at 25mg) and cannot fit it all into a column. A kit example is this: https://www.qiagen.com/ca/products/discovery-and-translational-research/dna-rna-purification/dna-purification/genomic-dna/gentra-puregene-tissue-kit/

But it is backordered. I was wondering if anyone knows of alternatives?

With pulmonary TB so prevalent in our part of the world we seen a lot of cases of acute abdomen, or abdominal ascitis or strictures where our suspicion is high for abdominal TB but diagnosing it becomes a challenege. What are the protocols being followed at your institute to diagnose abdominal TB?

I've read articles about deccelluralizing human lungs and recellularizing them with recipients' cells,

or gene editing porcine lungs to be accepted by humans.

But I couldn't find any where porcine lung scaffold is cellularized with recipient's humane cells.

Most serious complication of Covid-19 infection is internal blood clotting particularly in pulmonary tissue endangering life. Early detection of the clotting process can prevent many cases of mortality due to pulmonary tissue thrombosis. During Covid-19 or immediately after nonspecific symptoms (like fever, myalgia or cough )of the infection disappear gradual clotting of blood may import serious complication like pulmonary thrombosis and resulting increase mortality. Increased ferritin or D-dimer level is somewhat a late finding of blood clotting. So, abnormal platelet clumping in peripheral blood should be the earliest finding of internal blood clotting or DVT. We observed in two female cases ( age 42 and 55) of clinically diagnosed DVT leg that peripheral blood shows abnormal clumping. We started rivaroxaban 10mg daily and found gradual improvement. The drug was continued for 4 weeks. Both the patients are now doing well.

Today, it is possible to scan the human body very precisely with the help of x-ray CT images. These CT images are based on voxels. If you look closely at CT images of lung lobes, you can see five lobes segments, the right lung is divided into three lobes segments and the left lung is divided into two lobes segments. The MIP method is one of the most used techniques to define a slap thickness and visualize the desired 2D rendering voxels of lung lobes.

The biggest challenge in this case is how to delete or correct an exact location or a false segmentation of lung lobes without affecting the whole lobes segmentation?

is there a mech technique (like Delaunay Triangulation), which can mesh the entire 2D rendering lung lobesand then select and delete or correct the exact location manually without affecting the lobes segmentation?

i'm working on Emphysema animal model, i'm looking for lung inflamamtion and alveolar wall destruction , is it necessary to do lung perfusion with PBS before lung isolation ? to ovoid having blood in my histological slides

i'm wondering also if the perfusion can affect the lung inflammation and alveolar wall architecture ?

thank you

Hi,

I have simple quick question. Can I make a measurement of mouse lung to evaluate lung metastasis after formalin fixation? Is it not reliable?

Thanks.

Overall, the Spanish Flu is likely to be deadliest epidemic in the history of world. Estimates are that 1-3% of the world’s population died from the Spanish Flu. So many younger people died in the US in 1918 that the average US life expectancy was reduced by 10 years.

It is not clear why the second wave of the virus was so much more lethal than the first. There is some speculation that there may have been a mild and deadly version of the virus, but this has not been definitively confirmed. In the ‘developed’ world, the mortality rate was generally believed to be about 2%. In other counties, the mortality rate has been estimated to have caused up 14% of a population (Fiji islands) to die.

Eventually, toward the end of 1918 the number of deaths caused by the virus began to decrease. This is believed to be because there were so many people that had already been infected and/or the virus may have mutated again to be less invasive to the lungs. It eventually ‘devolved’ to be part of the seasonal flu. There was never a vaccine developed for the Spanish Flu.

As London was on lockdown A simple effective "near zero cost solution".... individual setotype- specific vaccination as the disease progression is slow, using the infecting virus as " inactivated virus" as vaccine. Is the base idea.   It's worth the time as it saves money and helps people  globally, ... This is not the accepted scientific format  It's almost zero cost.... Proving this in lab will take two ( three) years. But as Emergency Use Authorization it can be proven in 15 days. In the field by implementing steam inhalation as mentioned below.  Controlling Covid 19 globally - simple statergy Individual Serotype specific Vaccination : using steam inhalation & withholding breath. Doing steam inhalation three times a day for 5 minutes. " *Michael's method* " After inhaling steam "try to *withhold it there and allow CO2* levels to build till your comfort level say 30 seconds or less" is  possible . This will really help like vaccine serotype specific vaccine..  Technical steps  1. From day 0 of infection to serious symptoms takes day 8? to 10 if hospitalization is needed.  2 antibodies IgM production starts on day five after immunization/infection. 3. Crux of present statergy : not published : Theoretically steam inhalation moisture the water inhibits ATP synthesis so essentially needed for replication of the virus in the lung. ( Steam temperature theory is crap....as it will kill lung cells.)  4. As synthesis of the virus is inhibited. By disrupting the ATP synthesis, our immune mechanism take over...before the disease begin. at low viral loads when steam is being inhaled three times a day. 5. High altitude above 2500- 3000 Meters due to low CO2 acidosis is interfering in viral replication...acids can be used to inactivate viruses... so by with holding breath less than 30 seconds and steam inhalation we can control.  This can be experimented at small level at small geographic population. then applied at larger This can be experimented at small level then applied at larger level. Say a Taluk, or a small population...then a district or state larger then Global.  It's sure shot. I tried writing every where but then decided this is the ideal method ...( My relatives with whom I was in touch at home there names are also there. , Especially want to place on record STOP VIOLENCE AGAINST DOCTORS for providing all technical info on course of disease and treatment, addressing queries late night amidst hectic schedules. I was free had time to fix puzzle, collecting info on doubts... ) Incase of queries comments we can discuss ...it should be 97+% effective. Those with strong reaction to SARS-COV2 virus, how they respond an not sure ... Some serum profiles have issues.but as immune mechanism is same this may be highly effective. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175867/ https://www.researchgate.net/publication/343262923 https://orcid.org/0000-0003-2005-2521 S Michael Durairaj Dr Vijay Richard, Dr Suma Varsha Thota, Dr Vijayshree Vardharajan, Titus Samuel, Ms Venilla, Ms, Gracia Christopher,  Dr Paul Blesson Dr Ruchi Sachdeva, Dr Himanshu Sharma,  Dr Elizabeth Agnes Britto.

Virologist,  

lung specialist

immunologist  & 

biochemist (Oxidative phosphorylation {ATP} inhibition studies) to look at this togethar. 

maybe we may be able to check the spread of the virus and also control infection in 98% of the population.