Science topics: Pulmonary
Science topic

Pulmonary - Science topic

Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
Questions related to Pulmonary
  • asked a question related to Pulmonary
Question
5 answers
I would like to participate in publishing systematic reviews and meta-anlysis in my research field (public health) and longing to join research teams working with this kind of methods. I would appreciate any advice or guidance. Thanks
Relevant answer
Answer
I am also a dr lecturer at a university in Turkey. If you want to work with data envelopment analysis, we can sign a joint study. We need health data. You can check the official statistics sites of the country where you are located. If anyone is interested, I would like to help. We can also do studies about Turkey, and you can write down which analyzes you are good at. Thanks...
  • asked a question related to Pulmonary
Question
1 answer
Hi !
i m doing research and wish to know if their is stewardship In TB?
i understand that their is stewardship in extra pulmonary Tb and Drug resistant TB only !
Relevant answer
Answer
I don’t know your country. I think India heath care system has this kind of Stewardship of Anti-tuberculosis. I have found a literature titled : ''Anti-tuberculosis treatment stewardship in a private tertiary care hospital in South India''... They had a Stewardship team. Correspondence are from Amrita Institute of medical Sciences and Research Centre. You may google it to find their address or number.
  • asked a question related to Pulmonary
Question
2 answers
I'm looking for some lineage of human pulmonary endothelial cells that isn't from patients with adenocarcinoma. May you help me by suggesting any?
Thanks!!
Relevant answer
Answer
Thank you!!!
  • asked a question related to Pulmonary
Question
8 answers
Hi all,
I'm growing primary basal cells from lung digests, and was wondering if anyone has recommendations for cell freezing media for primary lung basal cells.
Previously I have been using Cryostor CS10, however I'm finding quite costly and would like to make my own.
My expansion media is Pneumacult EX Plus.
Many thanks,
Sam
Relevant answer
Answer
My pleasure! Let us know how your cells recover from freeze-thaw and how it compares to the current freezing medium!
Cheers,
Martine
  • asked a question related to Pulmonary
Question
4 answers
I am working on a rare case of mesothelioma and I am interested in knowing the epidemiology, especially in African countries
Relevant answer
Answer
malignant mesothelioma in India is quite rare, according to Indian council of medical research, cancer registry the reported incidence is 0.05-0.08 and 0.05-0.1per 100,000 in men and women respectively
  • asked a question related to Pulmonary
Question
5 answers
Need advice: Patient 37 y.o. with DVT and thrombosis of right radial artery (after coronary angio 2 weeks ago), pulmonary embolism and thrombolysis month ago. Now patient has indication for on-pump cardiac surgery - resection of postinfarction aneurysm of LV with thrombectomy (NB! Floating thrombus in the LV). Patient receives anticoagulants. Mild form of COVID-19 - 3 weeks ago.
What tactic is better to choose: operate now or wait 3-6 months after pulmonary embolism?
Thank you in advance!
Relevant answer
Answer
I think it's Right Decision .Delay the surgery for atleast 3 month
  • asked a question related to Pulmonary
Question
4 answers
I recently treated mice with clodronate liposomes but there seems to be no significant depletion of macrophages in the lungs. what could be the cause
Relevant answer
Answer
There are lot of reason, not to be effective of inj clodronate. I have described technical issue earlier
  • asked a question related to Pulmonary
Question
2 answers
I've read many posts and articles touting the use of externally applied low dose radiation to the lungs to address the inflammation brought on by COVID.
Since, as pointed out in our paper on the possibility of a direct anti-viral impact from xenon-133, nuclear medicine lung ventilation has the unique ability to bring ionizing radiation to the entire respiratory system, shouldn't it be explored as a means to provide a more targeted low dose therapy than available from an external beam approach?
Relevant answer
Answer
You raise a very interesting idea. I have heard that Xe-133 is much more common in USA and less in some places. For example, for V/Qs in Europe, there are a lot of places and practices that use Technegas. Does your suggestion also apply to Technegas?
  • asked a question related to Pulmonary
Question
3 answers
Lungs MRI images are needed for detecting CIVID -19.
Relevant answer
Answer
MRI lung for COVID-19 patients is not first choice but MRI signal in any pathology is measure as Intensity like Hyperintense or Hypointense lesion . CT scan is usually preferred modality for details pathology in COVID patients but MRI can be used as alternative to CT in Pregnant patients where ionization is harmful .
  • asked a question related to Pulmonary
Question
5 answers
We need to quantify the lung 🫁 damage caused by chronic silicosis among silica dust exposed workers in various occupational set up. We need some realistic idea including novel ideas for approximate estimation. Seeking your help.
Relevant answer
Answer
Kamalesh Sarkar I think the question is legitimate, it is time to review the classification of this disease and a new modelling of our vision regarding the assessment of its severity. I propose a systematic practice of respiratory and ventilatory functional tests. We still have to answer the question: which test predicts severity better and how will we classify patients?
  • asked a question related to Pulmonary
Question
1 answer
A project in my lab involves single cell RNA-seq data analysis of mouse whole lung samples. However, when we analyzed and clustered the dataset and searched for markers to annotate the group, there appears to be few to no cells exhibiting the classical epithelial cell markers EPCAM and CDH1.
Each sample has around 8000 cells after filtering for mitochondrial content, and the overall quality seems fine. But over half of the cells appear to be immune cells and there were less than 100 epithelial cells for each sample.
The mouse models are established by a collaborating group, and whole lung samples are sent to a sequencing company (travel time about 3 hours minimum) to generate the scRNA data. Our collaborators have adjusted experimental protocols multiple times to increase cell viability (~85%), but we are having difficulty fixing this lack of epithelial cells.
Does anyone have some experience with this, or know why there would be so few epithelial cells in scRNA-seq data of mouse lung samples?
Both my lab and our collaborators are fairly new to handling scRNA-seq data, so any insight would be helpful.
Relevant answer
Answer
Hi Yeogha,
Firstly, what proportion of your cells do you expect to be epithelial? From my understanding of human lungs, a large proportion of the cell types are mesenchymal, immune, or endothelial - so your results might not be that unreasonable.
Secondly, have you tried any of the canonical markers of the epithelial subtypes? p63, KRT5, MUC5AC, SCGB1A1, FOXJ1 for example?
In saying that, I know very little about scRNA-seq too, so I'm sure someone else will have a much more sensible answer!
All the best,
Sam
  • asked a question related to Pulmonary
Question
94 answers
Coronavirus disease (COVID-19) pandemic is a global health problem. Infected patients usually have respiratory symptoms due to lung involvements. However, liver impairments could be another findings.
So does Covid-19 affect liver functions & how?
Relevant answer
Answer
  • asked a question related to Pulmonary
Question
4 answers
A 65 years old male patient, once a heavy smoker and HBV carrier suffers from some form of cancer with pericardial sac metastasis.The PET/CT shows there are four nodules with high SUVmax (higher then 2.5 ).One of them locates in the lower lob of right lung,16.2×10.9 mm in size,with SUVmax=3.5.The rest three nodules, adjacent to mediastina, are bigger in size(21.6×20.6 mm,33.4×18.8mm and 30.2×30.0 respecttively) and higher in SUVmax( 8.7,5.8 and 7.1 respectively).All the other organs in the abdominal cavity are normal,except for liver.There is a nodule,24.3×18.2mm in size,with SUVmax=1.6. The IHC result of the cells collected from pericardial effusion shows:TTF-1(-),Naspin-A(-),CK5/6(-),CD56(-),Ki67(5% +),CK7(+),CK20(-),P40(-),Calretinin(-),WT1(-),PAX8(-),TTF-1(-),CDX2-88(-),GATA3(-),NKX3.1(-),Claudin4(mostly+),MOC31(-),Syn(-).The question is what kind of cancer is it,a adenoma, squamous cell cancer or neuroendocrine tumors?The other question is the histological origin of these metastases, the lung or the liver?Can any one give me some advice?Thank you!
Relevant answer
Answer
Apologies for not replying sooner about this very interesting case?
What was the eventual diagnosis (and how was this established) and what was the outcome?
  • asked a question related to Pulmonary
Question
1 answer
Hi everyone.
I want to establish Precision-cut lung slices (PCLS) by using peripheral lung, for example about a 3-cm specimen.
Can I get PCLS by injection 1.5% low melting agarose to the lungs?
Thank you!
Relevant answer
Answer
Dear Hikaru
Did you ever figure out how to inject the agarose ? My lab is interested in trying this for human and pig lung.
Thank you
Val
  • asked a question related to Pulmonary
Question
2 answers
I am trying to check the effeciency of my segmentation results via CT images. I am looking for a publicly available and annotated dataset of ct images for lung segmetnation. I would be great if the iamges in the dataset were in easy format such as JPEG.
Are there any seggestions?
Thank you in advance,
Relevant answer
Answer
As a confirmation of Yueh's opinion, I would also suggest using one of the public datasets and then using plugins (I recommend ImageJ/Fiji's plugins) to simply convert them into your desired format. It would be way easier/faster than digging into the web to find the appropriate type of data.
  • asked a question related to Pulmonary
Question
3 answers
Because as we know a smoker has less healthy lung than someone who doesnt
Relevant answer
Answer
After stop smoking the lung start to improve after few weeks to few months
the cilia start to repair .
you should start lung exercise to get rid of the sputum , take medication to get rid of sputum and cough , avoid pollution, eat healthy food
  • asked a question related to Pulmonary
Question
3 answers
Hi,
Im trying to isolate intratestinal macrophages from mouse lungs, and grow them in culture for a few days, to collect secretome. Does somebody have a good protocol for it?
I tried face sorting but the cells didn't grow well and died in the following days.
I also tried to separate f4/80 cells with macs columns but didn't get any separation.
Can someone have any suggestions?
Thank you,
Shani
Relevant answer
Answer
Well in that case, you should try supplementing with r-MCSF or L929 supernatant in the cultures. Macrophages are kinda dependent on this growth factor. For more information, please see:
Good luck!
  • asked a question related to Pulmonary
Question
3 answers
Please suggest some nebulizer/inhaler which specifically administer the nanoparticles into the rat/mice lungs.
Is there any company makes such device for rat/mice ?
Relevant answer
Answer
Inhaled route is preferred choice because of achieving high concentration in lung with manageable systemic side effects
  • asked a question related to Pulmonary
Question
2 answers
Hi,
We are doing focused scSeq of lung cancer and we need a panel of genes to identify and differentiate the lung epithelial cells from other cell types of lung. What genes would be useful?
Best regards,
Morten
Relevant answer
Answer
Increased activation of the TRPM8 variant in human lung epithelial cells leads to increased expression of several cytokine and chemokine genes, including IL-1alpha, -1beta, -4, -6, -8, and -13, granulocyte-macrophage colony-stimulating factor (GM-CSF), and TNF-alpha
Sabnis, A. S., Reilly, C. A., Veranth, J. M., & Yost, G. S. (2008). Increased transcription of cytokine genes in human lung epithelial cells through activation of a TRPM8 variant by cold temperatures. American journal of physiology. Lung cellular and molecular physiology, 295(1), L194–L200. https://doi.org/10.1152/ajplung.00072.2008
  • asked a question related to Pulmonary
Question
1 answer
Some of the Authors considered either sinusoidal function or breathing waveform. Anybody can suggest the exact, realistic breathing condition for the inhalation and exhalation to simulate particle deposition in a human lung airway?
Relevant answer
Answer
Ideally normal Tidal volume inhalation and exhalation are more physiological but to simulate particle get deposited in airway depend on particle size , smaller size particle less than 6 micron usually deposited in upper airway and larger particle size more than 6 micron deposited in small airways due to gravity effect but If we deep breath even small particle as well as large particle get deposited in small airway
  • asked a question related to Pulmonary
Question
1 answer
In COVID 19 detection if we will separate out clevicals and ribs in lung images.Weather we can improve accuracy?
Relevant answer
Answer
May be
  • asked a question related to Pulmonary
Question
2 answers
Hello-
I have been doing BAL fluid collections on mice, with the intention to extract RNA from the cell pellet. I know there are many ways to do this, but the optimal method I landed on is to use 3mls of PBS, one at a time. I've been lavaging/washing each ml 3 times before finally aspirating it and collecting it in a tube. With this method, I was able to get a big enough pellet to get sufficient RNA from a single naive mouse, and have been able to consistently replicate that.
The next step was doing this on mice that were treated with something that causes lung inflammation. I've tried it twice and both time, the RNA yield was significantly lower. I did notice that more of the PBS ended up in the thoracic cavity, so I imagine that's where the cells went and why I was unable to get more RNA.
So, my questions are: is it likely that I didn't do the lavage gently enough, for damaged lungs? Should I limit each ml to one or two washes, or use smaller amounts at a time? Do I need to dispense and aspirate the PBS more slowly? How can I maximize my cell yield in this situation?
Thank you-
Amy
Relevant answer
Answer
Amy,
One method that we use is gravity-assisted lavage. Look into it and see if that might increase your yield.
Best,
Franck
  • asked a question related to Pulmonary
Question
4 answers
The characterization of heavy metals like gadolinium in organs like liver, brain, lungs can best and simply be done by.....................
Relevant answer
Answer
Thank you, your responses and effort are appreciated.
  • asked a question related to Pulmonary
Question
2 answers
Hi, I need to transfect mice lung fibroblasts with lenti-cre. I bought one from a company but it didn't work at all, efficiency's super low. Can anyone recommend the premade lentil-cre particles that actually work? I'm in US. Many thanks!
Relevant answer
Answer
Vector Builder is great if you want to customize further, or this one:
Try multiple viral titers for 6-8h.
All the best!
  • asked a question related to Pulmonary
Question
1 answer
What Is The Relationship Between Vibration Of The Chest Wall And The Resulting Chest Wall Forces, Chest Wall Circumference, Intrapleural Pressure, And Expiratory Flow Rate?
Relevant answer
Answer
Hi Thomas,
You might need to read Dr. Bredge McCarren and the research that is published by this group. You will find answers in these publications.
Group has shown that the expiratory flow rate produced by vibration is approximately equal to the sum of the flow rates due to recoil of the lung and due to the compression and oscillation components of vibration https://pubmed.ncbi.nlm.nih.gov/16515421/ PMID: 16515421
In terms of an intrapleural pressure (IPP), what is proposed is that vibrational forces applied to the chest wall are transmitted to the lungs and airways. Also, that high frequency oscillation component of vibration influences IPP and expiration. An application of vibration during the expiratory phase increases expiratory flow rates PMID: 17132121
These forces increase IPP, which in turn increases expiratory flow rate hence helping to mechanically unload secretions (if you are interested in this).
I am interested how these forces influence hemodynamics. It looks like, they decrease an afterload, if applied during expiration.
Good luck with your studies, hope this helps.
Filip
You can also connect with the group on RG
  • asked a question related to Pulmonary
Question
11 answers
Although most people with COVID-19 have mild to moderate symptoms, the disease can cause severe medical complications and lead to death in some people. Older adults or people with existing chronic medical conditions are at greater risk of becoming seriously ill with COVID-19.
Complications can include:
  • Pneumonia and trouble breathing
  • Organ failure in several organs
  • Heart problems
  • A severe lung condition that causes a low amount of oxygen to go through your bloodstream to your organs (acute respiratory distress syndrome)
  • Blood clots
  • Acute kidney injury
  • Additional viral and bacterial infections
Relevant answer
Answer
Also see the following attached document for more insight.
  • asked a question related to Pulmonary
Question
1 answer
Hello,
I am running airflow simulation through a model of lungs. I want to find the velocities and pressures at certain locations. I tried creating ISO-surfaces but this surface cuts through the whole geometry and I want only specific locations on the geometry as shown by the colored lines in the figure. Is there a way to create a plane at the location of these lines exactly that will give only this branch? I want to find the velocities and pressures at the end of each branch.
Thank you in advance
Relevant answer
Answer
Have you figured out the solution? If yes please share it. I am stuck with the same problem.
  • asked a question related to Pulmonary
Question
2 answers
Hi all-
In our lab we work with human lung biopsies in which we digest and plate on fibroblasts to produce beautiful clones.
After the clone shave grown for 7-14 days we trypsinize and resuspend in StemCell Cryostor.
We have never had a problem when thawing and re-plating, but recently we haven't been able to regrow the cells after thawing to the full extent as the could be.
Taking any and all suggestions if you have run into this problem or if you have a protocol for freezing down and thawing lung epithelial cells that works wonderful for re-plating.
Thanks
Relevant answer
Answer
Dear Kiana,
It seems you are using a primary culture, isolated by yourself from the biopsies. The properties of primary cultures are much different from established cell lines. Primary cultures usually exhaust after several passages. They also may not withstand the harsh condition of freezing for several times. However, it is a undeniable fact that cells are hard to regrow after long-time freezings and you should be patient with these cells. some times they need 2-3 weeks to regrow and become refresh. My suggestion to you is being patient with the cells, even if you only a very low cell number in your culture vessel. I also suggest increasing the concentration of PBS in your medium to 15% and using glutamine supplement. This helps your cells to regrow faster.
  • asked a question related to Pulmonary
Question
2 answers
Because surgery methods used during tumor resection induce a blood accumulation in non lesional lung that we recovered, we wondered if someone know how to eliminate blood before lung digestion ? In order to avoid a contamination of tissu resident cells by circulating cells which can change the frequency of cells analyzed by flow cytometry ?
I look forward to reading your answers
Relevant answer
Answer
Hi Edouard, do you use human or mouse samples?
  • asked a question related to Pulmonary
Question
5 answers
Dear fellows,
I am looking for an expert in lung histology to collaborate on one of my project.
The expected work is to analyze histology images (e.g H&E, Trichrome, IHC etc) I have obtained from lung tissue from different mice models and provide me with an accurate description, quantification, an proper figure panels of whatever phenotype he/she will observe. The researcher will of analyze all the samples blindly.
For this work the chosen collaborator will of course be one of the co-authors of the future publication using this data.
look forward to here from you.
Best,
Yair
Relevant answer
اعطني قليلا من الوقت حتى اجد من يساعدك
شكرا جزيلا
  • asked a question related to Pulmonary
Question
1 answer
Hi,
I am currently looking into Trichrome stained section of mouse lung and trying to understand different parts of the lung but it is a bit confusing.
As far as I understood 1,2 are bronchiole, 3-pulmonary artery, 4- not sure, 5-also bronchiole?, 6-artery, 7- bronchiole as well?
Major confusion is between 1 2, 5 and 7 which all look like bronchiole but with different histology, and 4 which I can't clarify.
If anyone can distinguish these please let me know. Thank you so much in advance!
Relevant answer
Answer
Based on the images you provided, I would agree with your labels.
4 is probably an artery that was sectioned longitudinally rather than cross-sectioned, but I would need a higher magnification image to be sure.
1 and 2 both appear to be bronchioles. Most likely 2 is the start of a branch from 1. The upper label 5 in Lung_2.jpg is also a bronchiole.
What you labeled as 5 at the bottom of Lung_2.jpg, is a respiratory bronchiole. These are distunguished from other bronchioles by the opening into the alveolar space at the top right. Given the the large diameter, that likely also includes part of the bronchiole that it branches off. The U shaped structure in the middle of that image is a another example of a respiratory bronchiole.
7 is a bronchus (likely secondary or tertiary). The cartilage ring around bronchi is a major characteristic that distniguishes them from bronchioles. If you take a look at a higher magnification, you should also be able to see differences in the cells that line the airway (e.g. mucous producing cells present in the bronchi).
  • asked a question related to Pulmonary
Question
4 answers
For a depot injection designed for absorption by macrophage action, which injection route would be optimal to have most drug introduced into the lung by the lymph vessel?
Relevant answer
Answer
Dear Dr Min Gui Jang . See the following useful RG link:
  • asked a question related to Pulmonary
Question
3 answers
I am working on a project to classify lung CT images using CNN, and
as I understand, the pixel spacing is distance between the center of each pixel, specified by a numeric pair - adjacent row spacing and adjacent column spacing in mm (this is in 2D space), but because the CT images are 3D, the pixel spacing shows a triple value, and I thought that the second and third value are row pixel spacing and column pixel spacing, but the first value is Z dimension which means slice thickness, I am right or not?
the another question is: as it's known, CT scans has a different slice thickness, if I work on 2D slice of CT, I need to make all slice the same thickness or its only necessary when working on 3D scans?
  • asked a question related to Pulmonary
Question
10 answers
Research coming out now supports the idea that initial Viral Load has an effect on the eventual outcome of disease. A person exposed to a lower initial number of COVID-19 Viruses, has a better chance of their immune system being able to eliminate the Virus while it is still present in the Nasal Passages and Throat, as opposed to having the Virus spread to the lower respiratory tract and then begin causing lung involvement. For many it appears small numbers of the Virus manage to spread all along the upper and lower respiratory tract, and cause a full myriad of symptoms but the disease ends up remaining mild.
Relevant answer
  • asked a question related to Pulmonary
Question
4 answers
Hello !
We have one patient who presented pulmonary embolism and haemmorhagic stroke in same time.
So, anyone can help us for the management of anticoagulation ?
Relevant answer
Answer
This is a very challenging situation.
Can use sequential pneumatic compression for lower limbs. Also need to consider placement of an IVC filter if the patient has lower limb deep vein thrombosis.
Thereafter would discuss management of anticoagulation with a haematologist. Would then explain to the patient and their family the options for treatment and the potential risks and benefits.
  • asked a question related to Pulmonary
Question
3 answers
We see reduction in PA pressure after mitral commissurotomy and MVR
Does it persist in certain subset of patients?
Relevant answer
Dear Naeem Hameed,
It is an excellent question.
Mitral Stenosis is quite prevalent in our part of the world. In some patients with mitral stenosis pulmonary hypertension persists (PPH) after balloon valvoplasty or MVR.
Studies have shown PPH occurs more frequently in patients with residual significant gradient. Atrial fibrillation, old age, preexisting higher pulmonary hypertension, mitral regurgitation after procedure and left to right shunt created by septostomy etc. So it is difficult to say if there is permanent changes in pulmonary vascular bed in these patients as cause for pre or post capillary PH persist in many patients . Autopsy study have found plexiform change in the arteriole of these patients, but those reversed after procedures.
  • asked a question related to Pulmonary
Question
6 answers
We have many cases with normal D-dimer with the normal CT pulmonary angiography.
Relevant answer
If you have elevated d Dimer along with clinical features suggestive of pulmonary embolism and / or echo features of pulmonary hypertension but at the same time normal pulmonary CT, you should ask for pulmonary ventilation Perfusion scan (VQ scan). Because some of CTEPH are only detected by VQ scan.
  • asked a question related to Pulmonary
Question
1 answer
We are going to do mouse silica-induced lung fibrosis model. However, in the scientific papers the exact purchase details of e.g. DQ12, D50=2.2 um silica is missing. How dis you managed it when you did such models? I would appreciate some details. Thanks
Relevant answer
Answer
Hi Endre,
You may make an inquiry at Alfa Chemistry, they offer kinds of good-quality materials.
  • asked a question related to Pulmonary
Question
2 answers
Hi,
In my last experiment, I lysed RBC in a whole lung pellet with ACK. Even when I leave it for longer than 1 minute/ sample (protocol in my lab), the pellet is usually quite white. This time I noticed something grey at the bottom. Then I counted the WBC towards the end for my different groups I noticed there was a lot of debris. I'm not sure if it's the ACK or other enzymes in the lung digestion or the treatment I provided to the mice (I have never seen this until now). Any suggestions/guidance would be appreciated
Relevant answer
Answer
Hi Joakim Dahlin , sorry for the delay and thank you for your answer. This is a mouse sample. In previous experiments where I worked with ACK handling mice samples, I hadn't seen that color before. The treatment was my usual in vivo model that I had been using for over a year now. We decided to discard that ACK and get a new one and we haven't experience this ever since :)
  • asked a question related to Pulmonary
Question
3 answers
Please recommend buffer to homogenize lungs if I want to study phosphorylation of NfKb, STAT and IRF protein by Western Blot.
Relevant answer
Answer
Hi Chintan K Gandhi,
I always use GST buffer, it works very well for phosphorylation.
GST buffer (10mM MgCl2, 150mM NaCl, 1% NP40, 2% Glycerol, 1mM EDTA, 25mM HEPES pH7.5), with protease inhibitors
[1mM phenylmethylsulfonyl fluoride (PMSF), 10mM NaF, 1mM Na3VO4, and protease inhibitor cocktail (Amresco)].
Good luck
  • asked a question related to Pulmonary
Question
3 answers
Trying to segment X-ray images of lung using MATLAB. I want to segment all the images automatically, but with my code it can be applied only on single image. Getting issue with seed selection.
Relevant answer
Answer
This would be a great question to post in our new free medical imaging question and answer forum ( www.imagingQA.com ), there are already a few segmentation questions on there. If useful, please feel free to open a new topic at the link below :
  • asked a question related to Pulmonary
Question
4 answers
Post covid Lung fibrosis is hot topic now a days. I found most of the post covid shadow resolve without any medication. I hardly see actual case of Lung fibrosis specially after 12 weeks of discharge
Relevant answer
Answer
honeycombing really seems to be less frequent than other sequellae types (diffuse alveolar damage, cases which showed predominantly acute, organising or fibrosing patterns). We still can find patients getting septal and non septal lines, reticulations, bronchiectasis, localized fibrosis with no cranio-caudal gradient.
Talking about delay, these lesions take sometimes more than 12 weeks to appear.
Autopsy studies report a higher rate of fibrosing lesions ! still to debate.
DOI: 10.1111/his.14249
DOI: 10.12659/AJCR.926921
DOI: 10.1152/ajplung.00238.2020
  • asked a question related to Pulmonary
Question
15 answers
Ivermectin well-known drug - gave promising results in the treatment of Covid 19, although there is a concern, based on the fact that the concentration of free drug in the blood ( with safe doses ) is low compared to its in vitro IC50 against SARS-COV-2
My hypothesis is that some metabolites may be effective against the virus and accumulate inside the lung cells to reach the effective concentration. Is there any study that confirms or deny this hypothesis?
Relevant answer
Answer
Your hypothesis, that some metabolites may be effective against the virus, seems interesting. I don't think there is any study that has looked at this hypothesis.
  • asked a question related to Pulmonary
Question
2 answers
I have rat stomach samples that were confirmed to have adenocarcinoma. Similarly this was found in the lungs. How do i confirm that the adenocarcinoma in the lungs came from the stomach? Is mucin IHC staining suitable for this? Thanks
Relevant answer
Answer
Malcolm Nobre Thank you very much, it is very helpful :D
  • asked a question related to Pulmonary
Question
3 answers
Hi everyone,
I am looking for a public data set on RNAseq of human lung adenocarcinoma. I am looking at composition of microenvironment and particularly interested in DCs subsets within the tumour. I have never dealt with big data before and I have no idea if there is a public repository of this kind of data. Hope you can give me some insights!
kind regards,
Rita.
Relevant answer
Answer
TCGA, cbioportal
  • asked a question related to Pulmonary
Question
3 answers
I am looking for documented scientific articles about the role of physical therapy Pre and Post major surgeries.
Relevant answer
Answer
Dear Feras, the following papers may help you:
Boden I, Skinner EH, Browning L, et al. Preoperative physiotherapy for the prevention of respiratory complications after upper abdominal surgery: pragmatic, double blinded, multicentre randomised controlled trial. BMJ 2018;360:j5916. https://www.bmj.com/content/360/bmj.j5916.long
Bashir S, Siddiqi FA, Baig M, et al. Effect of chest physical therapy with early mobilization on post-operative pulmonary complications in upper abdominal surgeries. Rawal Medical Journal 2019;44(1):99-105. http://www.rmj.org.pk/fulltext/27-1536294286.pdf?1625217061
Wegner F, et al. Preoperative physical activity has a protective effect against postoperative pulmonary complications after abdominal surgery. Fisioterapia Brasil 2020;21(4):363-371. https://portalatlanticaeditora.com.br/index.php/fisioterapiabrasil/article/view/3971/pdf
Best wishes from Germany, Martin
  • asked a question related to Pulmonary
Question
4 answers
We read that people who breathe a lot of dust or asbestos can suffer from fibrosis in their lungs. I have seen that some people also suffer COPD without that and wondered if it has ever been related to high cholesterol? ie. Can fibrosis of the lungs be created from multiple tiny pulmonary embolisms over time when someone has high cholesterol?
Relevant answer
Answer
  • asked a question related to Pulmonary
Question
1 answer
Genetic profiling reveals an alarming rate of cross-contamination among human cell lines used in China and SPCA1 is one of these cell lines.
As the Expasy shows "Problematic cell line: Contaminated. Shown to be a HeLa derivative".Would it be ok if I continued to use SPCA for lung adenocarcinoma research?
Relevant answer
Answer
As you indicate yourself and as stated clearly in the Cellosaurus:
It is an HeLa contaminated cell line. It should therefore NOT be used in any context and especially not as a model of lung adenocarcinoma.
  • asked a question related to Pulmonary
Question
4 answers
Dear Colleagues,
I try to isolate Mycoplasma sp. from lung samples in rabbits but it is really difficult. Do you have any experience about that? Mycoplasma pulmonis was found just in mice but not in rabbits. Could you tell me what kind of spieces could I find? What kind of medium should I use?
Thanks in advance.
Silvia
Relevant answer
Answer
Sorry, my colleague told me all the findings
  • asked a question related to Pulmonary
Question
2 answers
Hi,
I'm facing 2 issues with our LLC and B16/F10 melanoma model in order to count lung metastasis.
In order to get significant metastasis, we need to incubate LLC and B16/F10 in mice for about a month. (LLC administration iv (tail vein): 5x10^5 cells/mouse / B16-F10: 1x10^6 cells/mouse IV).
At that endpoint, we obtain huge variation from mouse to mouse within the same experimental group.
How do you reduce this variation inside a same experimental group?
Is there another reliable cancer model to adresse metastasis in the lungs aside LLC/B16-F10 or urethane-induced?
thanks in advance
Relevant answer
Answer
Rémi Rosière : thanks a lot for the link. Best. Alexis
  • asked a question related to Pulmonary
Question
1 answer
Hi all,
I plan to do IHC to distinguish monocyte-derived macrophages from interstitial and alveolar macrophages in the lung in non-human primates, but I'm having a hard time finding a good marker. It seems that I can distinguish alveolar vs interstitial macrophages with CD206 and CD163, then maybe use CD14 for monocyte-derived macrophages, however, I'm seeing literature describing the loss of CD14 and gain of CD206 in monocyte-derived macrophages that have remained in the lung and begin to undergo "polarization" as replacement alveolar macrophages after infection. Anyone have suggestions or suggested reading?
Relevant answer
Answer
Hello,
I have some experience on murine alveolar macrophages. I was checking on some literatures on human alveolar macrophages and found the following article(take a look at Table6); SP-D may be a good candidate.
Hope this helps.
  • asked a question related to Pulmonary
Question
1 answer
I would like to culture the lung fibroblasts with TGF-b stimulation, but i don't know how to make sure the cell density, and i also have no idea about what kinds of plate (e.g. 6 wells plate? 12 wells?) or flask i could use to culture the cells with tgfb stimulation. Could you guys please give me some clues?
Relevant answer
Answer
Hi, it depend what do you want to do with your stimulate cells. For RNA extraction who could do it in 96 well plate and pool 3 or more well together or in 6 wells for protein extraction. For the density you have to try few to see which one is the best.
Hope it help!
  • asked a question related to Pulmonary
Question
4 answers
I am conducting a program evaluation plan for an outpatient pulmonary rehab program and am having trouble determining the statistical analysis methods to use. The following process and outcome evaluation questions I have are:
  1. How many days, on average, does it take a participant to enroll in class following referral/evaluation?
  2. What was the average number of initial evaluations conducted per week over the last year?
  3. What was the maximum class size over the last year?
  4. What percentage of program participants attended the pulmonary rehab program over the last year?
  5. What percentage of program participants are readmitted to the hospital within three months of completing the program?
  6. In what ways do the participants feel they are benefiting from program participation based on feedback from the feedback surveys?
Any help would be appreciated in determining the appropriate statistical test to use as well as the phrasing of questions. Thank you!
Relevant answer
Answer
Hello Arriana,
Most of the questions, as posted, sound to me as if ordinary descriptive statistics (and not a hypothesis test) would apply:
1. Days to action is frequently a skewed distribution. I'd suggest the Tukey five-number summary (minimum, lower hinge/quartile, median, upper hinge/quartile, maximum).
2. Mean & SD would suffice, unless the distribution is odd (as might be the case if these were rare events), in which case, 5-no. summary again.
3. Just report the maximum value; it is what is is! However, some sense of the distribution would be helpful, so 5-no. summary or histogram.
4. Simple descriptive value of the computed proportion.
5. Ditto.
6. How to summarize this depends on the survey structure. It could be as simple as reporting percents of cases who chose option "X"; it could be as complex as thematic analysis of open-ended or semi-structured interview responses.
If that still leaves you puzzled, I'd recommend trying to reach out to a local expert in evaluation/statistics to consult in deeper detail so that any features that you didn't mention in your query, but which may be germane to appropriate choice of analysis/reporting could be addressed.
Good luck with your work.
  • asked a question related to Pulmonary
Question
6 answers
Are there colleagues who would like to work on ground-glass opacity ("verre dépoli") evaluation using computed tomography (CT) imaging of the lungs ? The work can be extended to crazy paving (combining frosted glass, inter-lobular and intra-lobular cross-linking) evaluation. More details can be given later.
Relevant answer
Answer
Hi,
I would like to highlight a free, submillimetric, dataset made of 62 covid-positive CTs.
The dataset is provided alongside automatic labels (as well as the description of the strategy used to generate those labels).
The article is the following:
  • asked a question related to Pulmonary
Question
1 answer
I used several thresholding methods that were not helpful because they also remove the parts that are important for corona detection.
for example:
Thank you for introducing a simple and practical method for lung extraction from CT scan.
Relevant answer
Answer
One method that doesn't rely on the regional gas content in the lungs, but requires a training data set would be: Gerard SE … Reinhardt JM (2021). CT image segmentation for inflamed and fibrotic lungs using a multi-resolution convolutional neural network. Scientific Reports, 11(1). doi:10.1038/s41598-020-80936-4
On condition where it would be very important to check the results of this segmentation method is pleural effusion.
  • asked a question related to Pulmonary
Question
2 answers
Dear all,
I would like to measure the total lung SUV on Horos. Is it possible to segment lungs on CT and warp the segmented volume on the PET images to measure the total lung SUV?
Relevant answer
Answer
thank you , I will give it a try
  • asked a question related to Pulmonary
Question
2 answers
I'm currently working on a lung infection model for a project, and have tried EVERY method we can find for consistent intratracheal administration of our infection (biolite, small tubing down the trachea, catheters, feeding needles, you name it we've researched it.) It seems like the Penn Century microspray device is the top of the line in a well distributed and consistent infection and/or treatment, and we really want to use one.
Unfortunately we cant find one, anywhere. They went out of business in 2015. If your lab has one you rarely use, we'd love to buy it. We'd prefer not to purchase the $2000 one from Bio-Equip in China, they aren't licensed by penn century and we don't know what quality we would get (if you've ordered this other version, please tell me about it!)
Help?
Relevant answer
Answer
Aptar Pharma have developed a novel device to substitute Penn Century. The performance achieved is better. You can contact us for information and to purchase one. adam.fischer@aptar.com
  • asked a question related to Pulmonary
Question
13 answers
We do mouse lung imaging and inhomogeneous instillations are clearly visible.
Thus we are looking for a substitute for the Penn-century MicroSprayer® Aerosolizer because this company went out of business.
Any clue?
Thank you
Philippe
Relevant answer
Answer
APTAR Pharma have developed a substitute for the powder version. We are working on a liquid version.
You can contact us: adam.fischer@aptar.com
  • asked a question related to Pulmonary
Question
27 answers
Many suspected cases of Corona virus are self or home quarantine , some of them may have difficulty in breathing and other symptoms but are still having negative report for coronavirus disease. So what should be the advice given by Physiotherapist to them.
Relevant answer
Answer
An Update for COVID-19 patients and their families - April 16, 2021, as follows:
"Caring for Someone Sick at Home" (USA Center for Disease Control website):
Advice for caregivers in non-healthcare settings
Updated Apr. 16, 2021
Languages
Print
If you are caring for someone with COVID-19 at home or in a non-healthcare setting, follow this advice to protect yourself and others. Learn what to do when someone has symptoms of COVID-19 or when someone has been diagnosed with the virus. This information also should be followed when caring for people who have tested positive but are not showing symptoms.
*Note: Older adults and people of any age with serious underlying medical conditions are at higher risk for developing more severe illness from COVID-19. People at higher risk of severe illness should call their doctor as soon as symptoms start.
Provide support
Help cover basic needs
📷
Make sure the person who is sick drinks a lot of fluids and rests
  • Help the person who is sick follow their doctor’s instructions for care and medicine.For most people, symptoms last a few days, and people usually feel better after a week.
  • See if over-the-counter medicines for fever help the person feel better.
  • Make sure the person who is sick drinks a lot of fluids and rests.
  • Help them with grocery shopping, filling prescriptions, and getting other items they may need. Consider having the items delivered through a delivery service, if possible.
  • Take care of their pet(s), and limit contact between the person who is sick and their pet(s) when possible.
Watch for warning signs
  • Have their doctor’s phone number on hand.
  • Use CDC’s self-checker tool to help you make decisions about seeking appropriate medical care.
  • Call their doctor if the person keeps getting sicker. Call local emergency service and tell them that the person has or might have COVID-19.
When to seek emergency medical attention
Look for emergency warning signs* for COVID-19. If someone is showing any of these signs, seek emergency medical care immediately:
  • Trouble breathing
  • Persistent pain or pressure in the chest
  • New confusion
  • Inability to wake or stay awake
  • Pale, gray, or blue-colored skin, lips, or nail beds, depending on skin tone
*This list is not all possible symptoms. Please call your medical provider for any other symptoms that are severe or concerning to you.
Call 911 or call ahead to your local emergency facility: Notify the operator that you are seeking care for someone who has or may have COVID-19.
Protect yourself
In This Section
  • Limit contact
  • Eat in separate areas
  • Avoid sharing personal items
  • When to wear a mask or gloves
  • Clean your hands often
  • Track your own health
Limit contact
📷
Keep a separate bedroom and bathroom for a person who is sick
COVID-19 spreads between people who are in close contact (within about 6 feet) through respiratory droplets, created when someone talks, coughs or sneezes. Staying away from others helps stop the spread of COVID-19.
The caregiver, when possible, should not be someone who is at higher risk for severe illness from COVID-19.
The person who is sick should isolate
The sick person should separate themselves from others in the home. Learn when and how to isolate.
  • If possible, have the person who is sick use a separate bedroom and bathroom. If possible, have the person who is sick stay in their own “sick room” or area and away from others. Try to stay at least 6 feet away from the sick person.
  • Shared space: If you have to share space, make sure the room has good air flow.Open the window to increase air circulation. Improving ventilation helps remove respiratory droplets from the air.
  • Avoid having visitors. Avoid having any unnecessary visitors, especially visits by people who are at higher risk for severe illness.
Caregivers should quarantine
Caregivers and anyone who has been in close contact with someone who has COVID-19 should stay home, except in limited circumstances. Learn when and how to quarantine.
When it's safe for a person who has been sick to be around others
Deciding when it is safe to be around others is different for different situations. Find out when someone who is sick can safely end home isolation.
Eat in separate rooms or areas
  • Stay separated: The person who is sick should eat (or be fed) in their room, if possible.
  • Wash dishes and utensils using gloves and hot water: Handle any dishes, cups/glasses, or silverware used by the person who is sick with gloves. Wash them with soap and hot water or in a dishwasher.
  • Clean hands after taking off gloves or handling used items.
Avoid sharing personal items
  • Do not share: Do not share dishes, cups/glasses, silverware, towels, bedding, or electronics (like a cell phone) with the person who is sick.
When to wear a mask or gloves
The person who is sick
  • The person who is sick should wear a mask when they are around other people at home and out (including before they enter a doctor’s office).
  • The mask helps prevent a person who is sick from spreading the virus to others. It keeps respiratory droplets contained and from reaching other people.
  • Masks should not be placed on young children under age 2, anyone who has trouble breathing, or is not able to remove the covering without help.
media iconLow Resolution Video
Caregiver
  • Put on a mask and ask the sick person to put on a mask  before entering the room.
  • Wear gloves when you touch or have contact with the sick person’s blood, stool, or body fluids, such as saliva, mucus, vomit, and urine. Throw out gloves into a lined trash can and wash your hands right away.Practice everyday preventive actions to keep from getting sick:  wash your hands often; avoid touching your eyes, nose, and mouth; and frequently clean and disinfect surfaces.
Note: During the COVID-19 pandemic, medical grade masks are reserved for healthcare workers and some first responders.
Clean your hands often
  • Wash hands: Wash your hands often with soap and water for at least 20 seconds. Tell everyone in the home to do the same, especially after being near the person who is sick.
  • Hand sanitizer: If soap and water are not readily available, use a hand sanitizer that contains at least 60% alcohol. Cover all surfaces of your hands and rub them together until they feel dry.
  • Hands off: Avoid touching your eyes, nose, and mouth with unwashed hands.
  • Learn more about handwashing.
When and how to clean surfaces and objects
Cleaning with a household cleaner that contains soap or detergent reduces the amount of germs on surfaces and objects and decreases risk of infection from surfaces. In most situations, cleaning alone removes most virus particles on surfaces.
  • Clean high-touch surfaces and objects regularly (for example, daily or after each use) and after you have visitors in your home.
  • Focus on high-touch surfaces and objects (doorknobs, tables, handles, light switches, phones, remote controls, and countertops).
  • Clean other surfaces in your home when they are visibly dirty or as needed. Clean them more frequently if people in your household are more likely to get very sick from COVID-19. Disinfect if certain conditions apply.
  • Clean surfaces using a product suitable for each surface, following instructions on the product label.
When Someone Is Sick
If someone in your home is sick or someone who has COVID-19 has been in your home in the last 24 hours, clean and disinfect your home. Disinfecting removes germs and reduces their spread. See Caring for Someone Who Is Sick at Home for more information.
For more information on cleaning and disinfecting safely, see Cleaning and Disinfecting Your Home.
Track your own health
  • Caregivers should stay home and monitor their health for COVID-19 symptoms while caring for the person who is sick.Symptoms include fever, cough, and shortness of breath but other symptoms may be present as well. Trouble breathing is a more serious warning sign that you need medical attention.
  • Caregivers should continue to stay home after care is complete. Caregivers can leave their home 14 days after their last close contact with the person who is sick (based on the time it takes to develop illness), or 14 days after the person who is sick meets the criteria to end home isolation.
  • The best way to protect yourself and others is to stay home for 14 days if you think you’ve been exposed to someone who has COVID-19. Check your local health department’s website for information about options in your area to possibly shorten this quarantine period.
  • Use CDC’s self-checker tool to help you make decisions about seeking appropriate medical care."
  • www.cdc.gov/coronavirus/2019-ncov/if-you-are-sick/care-for-someone.html
  • asked a question related to Pulmonary
Question
1 answer
I'm having trouble with a nuclear protein immunostain of cells (lung fibroblasts) cultured on soft (1 kPa) collagen-coated hydrogels. Can anyone give me a suggestion?
Relevant answer
Answer
Hello, Fernanda!
Please You look at the following articles:
IMR-90 cells (ATCC), from a frozen stock
Immunocytochemistry
  • asked a question related to Pulmonary
Question
3 answers
Hey,
I am currently investigating acute respiratory distress syndrome (ARDS) in mice via intra-tracheal infection of mouse lungs with E.coli. Thus far I have validated our model using histological approaches. However, I would also like to have some real time monitoring to assess the progression of the infection/ARDS symptoms. I have been doing peripheral blood oxygen measurements. However the SpO2 % measurements can have high variability across my experiment. the pulse oximeter we use takes quite a long time to read a stable measurement, sometimes I can be stuck measuring a single mouse for 20 minutes. Often, I get two stable readings from my mice. For example, control uninfected mice ideally would have 99% SpO2 saturation. however I can also get a clean stable reading at ~85%. For my infected mice, the values vary. Since there is no standard, its quite tricky to determine which measurement is true. For example, an infected mouse, 6 hours post infection, can have a reading of 77% SpO2 and can then immediately read stably, at 50%.
I am aware of ensuring the mouse is accustomed to being restrained and recovers from that stress before measure blood oxygen. In addition, extremely low stable readings (60%<) can suggest a mouse may need to be euthanized, but qualitative assessment of the mice, exhibits they are are fine and very active, so it makes me doubt extremely low but stable readings.
Has anyone had issues with getting accurate SpO2 readings with mice and have any tips for accurate measurement and interpretation?
Relevant answer
Answer
Hey,
first of all body weight loss correlates with the degree of disease. Furthermore, you can evaluate the degree of acute lung injury via albumin and protein levels in BALF in addition to histopathology. For the inflammatory response in the lungs suggest cytokine levels and amount of immune cells in BALF. We used these methods in 2 mouse models of ARDS recently:
Best,
Christian
  • asked a question related to Pulmonary
Question
5 answers
I did Immunofluorescence staining of DAPI using the lung tumor tissue frozen section which contains part of the normal site, but I find it hard to figure out why the DAPI staining (or other antibodies) intensity was weaker compared to the normal sites under the same condition. I repeated it more than once, I don't know what's wrong with it.
Relevant answer
Answer
I am a little bit confused that after the sectioning, the antigens are all exposed on the surface, is there still a problem of antibody penetration? Oscar L. Sierra Philip Patrick Yost
  • asked a question related to Pulmonary
Question
5 answers
I know in the literature all papers that describe syllable structures in Arabic, referred to the following five patterns:
CV, CVV, CVC, CVVC, CVCC, and in some dialects CCV(V)C(C).
However, all these papers considered VV as the longe vowel, NOT two different vowels in a sequence!!
Therefore, could we consider Arabic as a language with only three core syllabic structures: CV, CVC, CVCC, where the CC is only a true consonant cluster with two different consonants, and V is the vowel either long or short???
Relevant answer
Answer
I agree with Hafida Rahsl Bouziane.
  • asked a question related to Pulmonary
Question
4 answers
Hello,
Searching for the ultimate housekeeping gene (with expression stability) that works well in human pulmonary fibroblasts (besides GAPDH), any suggestions?
Relevant answer
Answer
"Identification of stable housekeeping genes for real-time PCR in human pulmonary fibroblasts"
Hope this paper will give you more insight.
Masuma
  • asked a question related to Pulmonary
Question
2 answers
Hi all,
I have been experimenting with human lung fibroblasts and the effect of nintedanib and pirfenidone upon them in both normoxic and hypoxic (1% O2) conditions. I want to see the effects on ABCB1 expression therefore I am blotting for this protein. However, I see a faint band at the 140kDa range (correct band according to literature) but another stronger band just above 100kDa which I am struggling to account for. The literature suggests glycosylated protein may accumulate as a smear but this is seen at a higher range (180-300kDa). Some of the images from the websites selling the antibody show the band I am seeing, but none explain its presence.
If anyone has had any similar experience and could help me to identify this extra band it would be much appreciated.
Best wishes
Dr. Robert J. Lawrence
Relevant answer
Answer
My thought is that you are seeing a proteolytic fragment. If my memory is right, there is a highly protease-sensitive site about midway through the amino acid sequence.
  • asked a question related to Pulmonary
Question
2 answers
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at 25mg) and cannot fit it all into a column. A kit example is this: https://www.qiagen.com/ca/products/discovery-and-translational-research/dna-rna-purification/dna-purification/genomic-dna/gentra-puregene-tissue-kit/
But it is backordered. I was wondering if anyone knows of alternatives?
Relevant answer
Answer
Three typical options for RNA and DNA purification: organic extraction, spin columns, magnetic beads. For large sample input Trizol might be the best, if you dont want to deal with spin columns
  • asked a question related to Pulmonary
Question
2 answers
With pulmonary TB so prevalent in our part of the world we seen a lot of cases of acute abdomen, or abdominal ascitis or strictures where our suspicion is high for abdominal TB but diagnosing it becomes a challenege. What are the protocols being followed at your institute to diagnose abdominal TB?
Relevant answer
Answer
..GI Endoscopy
..Barium studies
..Peritoneal fluid microscopy, Gene Xpert and TB culture
..Peritoneal gross examination and biopsy
..abdominal lymph node biopsy
..look for chest/lungs or other organs involvement
..history of TB contact(may need good inquiry)
..symptoms of fever especially afternoon or evening/night, anorexia, +/-constipation/or diarrhea, weight loss, lethargy,...
These points may help upto great extent in making diagnosis...
If physician is convinced to start anti tuberculous treatment, good response may be noted in 15-45days in majority of patients...important exclusion include drug resistant tuberculosis and other illnesses closely related to TB.
Can consult nearby physician experienced in the diagnosis and management of TB.
  • asked a question related to Pulmonary
Question
1 answer
I've read articles about deccelluralizing human lungs and recellularizing them with recipients' cells,
or gene editing porcine lungs to be accepted by humans.
But I couldn't find any where porcine lung scaffold is cellularized with recipient's humane cells.
Relevant answer
Answer
  • asked a question related to Pulmonary
Question
8 answers
Most serious complication of Covid-19 infection is internal blood clotting particularly in pulmonary tissue endangering life. Early detection of the clotting process can prevent many cases of mortality due to pulmonary tissue thrombosis. During Covid-19 or immediately after nonspecific symptoms (like fever, myalgia or cough )of the infection disappear gradual clotting of blood may import serious complication like pulmonary thrombosis and resulting increase mortality. Increased ferritin or D-dimer level is somewhat a late finding of blood clotting. So, abnormal platelet clumping in peripheral blood should be the earliest finding of internal blood clotting or DVT. We observed in two female cases ( age 42 and 55) of clinically diagnosed DVT leg that peripheral blood shows abnormal clumping. We started rivaroxaban 10mg daily and found gradual improvement. The drug was continued for 4 weeks. Both the patients are now doing well.
Relevant answer
Answer
A vital question!
  • asked a question related to Pulmonary
Question
4 answers
Today, it is possible to scan the human body very precisely with the help of x-ray CT images. These CT images are based on voxels. If you look closely at CT images of lung lobes, you can see five lobes segments, the right lung is divided into three lobes segments and the left lung is divided into two lobes segments. The MIP method is one of the most used techniques to define a slap thickness and visualize the desired 2D rendering voxels of lung lobes.
The biggest challenge in this case is how to delete or correct an exact location or a false segmentation of lung lobes without affecting the whole lobes segmentation?
is there a mech technique (like Delaunay Triangulation), which can mesh the entire 2D rendering lung lobesand then select and delete or correct the exact location manually without affecting the lobes segmentation?
Relevant answer
Answer
Thank you. I have already read this paper, but it does not help, beacause I have already created volume mesh from CT scan using marching cubes. My problem now is to model a segmented Lung lobe with individualized surface meshes like in the following paper:
  • asked a question related to Pulmonary
Question
1 answer
i'm working on Emphysema animal model, i'm looking for lung inflamamtion and alveolar wall destruction , is it necessary to do lung perfusion with PBS before lung isolation ? to ovoid having blood in my histological slides
i'm wondering also if the perfusion can affect the lung inflammation and alveolar wall architecture ?
thank you
Relevant answer
Answer
It is suggested that you‘d better divide each group into two parts, one part of the animals to do alveolar lavage and the other part to take the organs directly
  • asked a question related to Pulmonary
Question
4 answers
Hi,
I have simple quick question. Can I make a measurement of mouse lung to evaluate lung metastasis after formalin fixation? Is it not reliable?
Thanks.
Relevant answer
Answer
Hello Kim,
If it is just for statistical comparison there is no problem. If your goal is to give weight+- standard error results, it's not fair. You can weigh other organs before fixation (exp n=6) and after to get an idea of the increase in weight caused by your fixative solution. Determining a coefficient will greatly help you to know the real weight before fixation. You must use the same tissue.
Regards
  • asked a question related to Pulmonary
Question
6 answers
Overall, the Spanish Flu is likely to be deadliest epidemic in the history of world. Estimates are that 1-3% of the world’s population died from the Spanish Flu. So many younger people died in the US in 1918 that the average US life expectancy was reduced by 10 years.
It is not clear why the second wave of the virus was so much more lethal than the first. There is some speculation that there may have been a mild and deadly version of the virus, but this has not been definitively confirmed. In the ‘developed’ world, the mortality rate was generally believed to be about 2%. In other counties, the mortality rate has been estimated to have caused up 14% of a population (Fiji islands) to die.
Eventually, toward the end of 1918 the number of deaths caused by the virus began to decrease. This is believed to be because there were so many people that had already been infected and/or the virus may have mutated again to be less invasive to the lungs. It eventually ‘devolved’ to be part of the seasonal flu. There was never a vaccine developed for the Spanish Flu.
Relevant answer
Answer
Mutation in virus always make it more dangerous as it adopt itself in the environment where it is growing.Duration of the mutation depends upon type of virus.
  • asked a question related to Pulmonary
Question
1 answer
As London was on lockdown A simple effective "near zero cost solution".... individual setotype- specific vaccination as the disease progression is slow, using the infecting virus as " inactivated virus" as vaccine. Is the base idea.   It's worth the time as it saves money and helps people  globally, ... This is not the accepted scientific format  It's almost zero cost.... Proving this in lab will take two ( three) years. But as Emergency Use Authorization it can be proven in 15 days. In the field by implementing steam inhalation as mentioned below.  Controlling Covid 19 globally - simple statergy Individual Serotype specific Vaccination : using steam inhalation & withholding breath. Doing steam inhalation three times a day for 5 minutes. " *Michael's method* " After inhaling steam "try to *withhold it there and allow CO2* levels to build till your comfort level say 30 seconds or less" is  possible . This will really help like vaccine serotype specific vaccine..  Technical steps  1. From day 0 of infection to serious symptoms takes day 8? to 10 if hospitalization is needed.  2 antibodies IgM production starts on day five after immunization/infection. 3. Crux of present statergy : not published : Theoretically steam inhalation moisture the water inhibits ATP synthesis so essentially needed for replication of the virus in the lung. ( Steam temperature theory is crap....as it will kill lung cells.)  4. As synthesis of the virus is inhibited. By disrupting the ATP synthesis, our immune mechanism take over...before the disease begin. at low viral loads when steam is being inhaled three times a day. 5. High altitude above 2500- 3000 Meters due to low CO2 acidosis is interfering in viral replication...acids can be used to inactivate viruses... so by with holding breath less than 30 seconds and steam inhalation we can control.  This can be experimented at small level at small geographic population. then applied at larger This can be experimented at small level then applied at larger level. Say a Taluk, or a small population...then a district or state larger then Global.  It's sure shot. I tried writing every where but then decided this is the ideal method ...( My relatives with whom I was in touch at home there names are also there. , Especially want to place on record STOP VIOLENCE AGAINST DOCTORS for providing all technical info on course of disease and treatment, addressing queries late night amidst hectic schedules. I was free had time to fix puzzle, collecting info on doubts... ) Incase of queries comments we can discuss ...it should be 97+% effective. Those with strong reaction to SARS-COV2 virus, how they respond an not sure ... Some serum profiles have issues.but as immune mechanism is same this may be highly effective. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175867/ https://www.researchgate.net/publication/343262923 https://orcid.org/0000-0003-2005-2521 S Michael Durairaj Dr Vijay Richard, Dr Suma Varsha Thota, Dr Vijayshree Vardharajan, Titus Samuel, Ms Venilla, Ms, Gracia Christopher,  Dr Paul Blesson Dr Ruchi Sachdeva, Dr Himanshu Sharma,  Dr Elizabeth Agnes Britto.
Virologist,  
lung specialist
immunologist  & 
biochemist (Oxidative phosphorylation {ATP} inhibition studies) to look at this togethar. 
maybe we may be able to check the spread of the virus and also control infection in 98% of the population.
Relevant answer
Answer
This is a way to address the current pandemic globally, as vaccination and also as control of spread of the virus.
SARS-COV2 virus can be inactivated during initial synthesis and used as vaccine as progression of virus infection is slow 8 to 10 days.
Dr Gustavo forwarded the idea.... for evaluation.... to Bangalore genomic centre
@ Prof. Dr. Gustavo Zubieta-Calleja Head High Altitude Pulmonary and Pathology Institute Av. Copacabana, Prolongacion # 55 Estacion Av. del Poeta Teleferico Celeste. La Paz, Bolivia https://goo.gl/maps/nL2Y2TJjd4t http://altitudeclinic.com Available for High Altitude Consultations: https://zuniv.net/IPPA1/Consultation.html