Protocol Writing - Science topic

Another Systematic Review (Computerized Rehab) for your reference:

Hi all,

Adverse events need to be recorded using a safety database (e.g. Argus, safety easy ...) and in a clinical trial setting in the trial database. AEs or SAEs need to be reported to the regulatory authority and to the ethics committee.

The response to the question is different if you are Sponsor, clinical trial centre or CRO.

If you are a member of a sponsor or a CRO, Pharmacovigilance is quite a large domain and I recommend you attend to pharmacovigilance (PV)training.

Here is the link to the PV good practices: https://www.ema.europa.eu/en/human-regulatory/post-authorisation/pharmacovigilance/good-pharmacovigilance-practices

Quite a lot of materials to read and master.

Hello,

This may be a helpful source of e-learning

Regards,

Alphonce Nsabi Simbila

You just refer to the methodology previously published but you can provide a summary and any updated information :)

I am not sure of your objective. So I am sharing on general guidance. I think you have missed to include standardization of equipment's which is a critical one in protocols. If you are collaborating with others check with their validation sheets to match yours. I have had issues with flow cytometry results with our collaborators that are very different from ours. Also if you are using standards, the manuf & expiry date, consistency of pdt, etc. A better protocol is one which has clarity of each step, has repeatability and reliability. To document commercial reagents in a standardized way, I use RRID portal.

Thank you so much

My suggestion would be at least 6 months prior to the birth parent delivery. I have had better success with larger volumes of milk. I also have used the SNS with the non-birth parent to assist with increasing milk supply when baby is born.

I'm finishing a new protocol using Inplasy.com, and I did not have any problems using the service. Probably, my protocol will be published in a couple of days. All my previous systematic review protocols were published in PROSPERO. However, PROSPERO is currently receiving an unprecedented number of records, and I had experienced a substantial delay in the registration of my last protocols.

Your research proposal is just that: A proposal of how you intend to go about your research. However all research is into the unknown, and factors that you did know at the time of writing the proposal will be uncovered by your research. In practice, nobody seems to publish proposals after the proposed research is over and done with. This is because the proposal is outdated by the research. The final report contains sufficient up-to-date details (including e.g., trade names) of the instruments and procedures actually used.

Hi Stefania,

We incubate cells at 37°C with radiolabeled antibody and at each time point separate supernatant, cell-bound fraction and cells (internalized fraction) for gamma-counting.

I agree with David C Schneider, if you do model checking and there is overdispersion, negative binomial reg will do.

Cheers

Collaboration is becoming more important over time. The body of scientific knowledge and technology is expanding so rapidly that it’s becoming more difficult for individual investigators to master the essential skills and knowledge necessary to produce currently relevant work.

Hi,

Here are several links to guidelines for writing intellectual biography:

academic.mu.edu/nowacek/documents/IntellectualAutobiography.doc

classroom.synonym.com › Classroom

Hi Catarina!

I am not sure if I got your question write... Why do you need "elaborate" the ELISA, there are no kit available? The quantification provided by the ELISA test is based in a standard and purified protein. Once you have a stable protein where you can be sure of the concentration, you can use the method you are trying to develop to quantify your samples based on that standard protein. Another point is how the color (colorimetric test) intensity is related to the concentration, and this relation is usually not linear. That is why is very useful use a kit that was tested for those conditions. And you shouldn't extrapolate points out of the curve, meaning not having concentrations that are not covered by the standard curve. In order to to that, you should run a first ELISA test with a few dilutions (1:10, 1:100, 1:500, for example). Until you find the dilution that give a result that is covered by the standard curve. After that, you will have an idea of the dilution factor necessary. I also worked with a high concentrated sample and after a few preliminar trials, I standardized and always run my samples in 2 concentrations (1:250 and 1:500). In this way, the results are always covered by the standard curve... Good luck!

Bioinformatics and Computational Biology are two completely different things.  Bioinformatics is mostly biostatistical methods.  Computational biology is balance equations and biokinetics.  For the first any stats text will work since the methods are the same independent of the application.  For the second try biological engineering textbooks and pharmacokinetics and drug metabolism texts.  Most biologist won't have the calculus background for computational biology.  I've tried teaching it to biologists and just gotten a chorus of "Why don't the d's cancel on the derivative?"

I do not have a written out protocol. But roughly: an overnight E. coli culture in LB will be around 1 x 10E9 cells/ml. Single colonies are accepted to be true single colonies if there are between 50 and 300 on a 9 cm petridish. So when plating 100 µl you need a dilution of about 10E6 To compensate for innacuracy in the initial estimate work around that e.g. 10E5 - 10E7. The attached scheme is for plating starting from soil samples but it illustrates a bit what I mean. 

Thanks to the people who responded offering to help translate the protocol.

For anyone interested, you can find the translated protocols at this site:

Also of interest, our Alternative Careers Group has put together a collection of career resources for PhDs in the life sciences with a special focus on working in France and Europe: