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Prosthetics - Science topic

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What would be a good journal to publish work on a new way to deduce the shape of an ocular prosthesis?
It seems that over the years some journals have come and gone that would be suitable to publish novel work in ophthalmic prosthetics (colloquially, glass eyes). The only active journal on the subject seems to be the American Society of Ocularists "Journal of Ophthalmic Prosthetics." Unfortunately it's not indexed and not available outside their membership, so publishing there is not my first choice.
Any recommendations appreciated!
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I would suggest "Eye". Even though they do not have a lot on prostheses and are more on native eyes, they might be interested in something like the topic you mention.
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What are the materials used in our conversation in the manufacture of prosthetic limbs for patients with hand amputations?
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I hope this article will help You advance in Your research https://habr.com/ru/post/394579.
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Hello, I need help to figure out how to work in the Working with Occlusal Fingerprint Analysis program. I read the instructions, but I can't figure out how to calculate the sliding of one jaw on the other?
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I will be working with OFA once I can figure out how to download the program. Could you tell me how you accessed the program? Every time I try to download it I get taken to a screen that needs a BONN log-in. I've emailed Kullmer and haven't heard back.
If you still haven't found the way to calculate jaw sliding I would definitely try and assist. Thanks in advance!
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I'm trying to cast microfluidics systems and bio prosthetics.
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Hey
Jasper
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Orolingual dyskinesias are often related to drugs such as dopamine receptor-blocking agents. It may occur in relation to neurodegenerative disorders (Huntington’s disease, neuroacanthocytosis) or neuropsychiatric conditions (chronic schizophrenia, Rett syndrome, dementia). Further, it also may be induced spontaneously or peripherally (edentulousness or ill-fitting prosthesis).
Occasionally dentists or oral surgeons experience improvement in orolingual dyskinesia after insertion of adequate dentures in patients who do not have dentures or have ill-fitting dentures. Sutcher et al. showed in their series of studies that dental prosthetic therapy is effective not only in spontaneous orofacial dyskinesia but also tardive orofacial dyskinesia. A video on YouTube shows a case in which orolingual dyskinesia significantly improved immediately after denture adjustment (https://youtu.be/Wn4YoFtPpjI). Naturally, dental prosthetic therapy is not effective in all cases with orolingual dyskinesia, but there are some cases in which it is very effective. When talking about this, some neurologists assert that it is a “superstition”. What do you think about this?
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Dear prosthodontic specialists, oral surgeons, practicing dentists,
Have you ever experienced cases where involuntary movements improved dramatically after prosthetic treatment?
I believe that dental professionals should participate in the diagnosis and treatment of involuntary movements in the stomatognathic region.
Kazuya Yoshida
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Hi everybody
I am simulating a static analysis of a prosthetic foot which consists of 10 solids:
- A pyramid adapter
- Three carbon fiber plates
- Two screws that join two plates with the pyramidal adapter
- Two pins that connect two plates to each other
- A polymeric base where everything is assembled
- A heel wedge that serves as a shock absorber
Attached of the ensemble (in Spanish)
The analysis consists of applying a vertical force on the pyramidal adapter of 2240 N, it also has bolt preloads of 10 N and 20 N in the respective screws and pins, and the polymeric base is fixed.
The question would be to choose the types of interactions and constraints in the model.
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Interesting. will it help in running also.
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Accelerated orthodontic tooth movement.
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VEGF though investigated in animal studies, has not been investigated sufficiently for human applications yet.
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-Good morning, my name is Rogger Cayo Bulnes, I am a medical student at the Peruvian University of Applied Sciences (UPC). Currently, together with my partner Isabel Chang Pardo we are conducting a thesis study entitled "Functional and psychosocial impact on patients who use 3D printed prostheses K-PIX and K-PIX ll of upper limb in 2021 in Peru: A number of cases".
To do this, we would like to use the instrument that you developed, the Prosthetic Upper Extremity Functional Index (PUFI). Therefore, I would like to ask you if you could share the survey with its instructions for use, and if there is an electronic version so that we can apply it to our target population. Please, if so, I would be eternally grateful.
If so, could you please send it to me at the following email
Thank you very much for your help and I say goodbye.
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Greets,
I think you‘ve mistaken me.
Keep up with the good work.
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I am working on a project related to sEMG classification. In order to process the signal, I am trying to filter the signal, especially power interference. The signal is acquired from Delsys Bagnoli 16, and the sample rate was 4000. I collected 6 signals simultaneously, and some signals showed this abnormal behaviour in fft (see the figure, zoomed to 0-700Hz). Therefore, I tried notch filters to remove the spikes, but it seems like it has other spikes around 50Hz. For example, see the next figure. I need to know the reason for this behaviour. Is it due to fft calculation? (see the last figure, Matlab code. it was recommended by the Matlab (https://www.youtube.com/watch?v=VFt3UVw7VrE , at 5:17)) or due to a filtering problem at the amplifier?
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A possibility: in many countries, the power supplied from an outlet is alternating current at 50Hz or 60Hz. Therefore, many electrical devices (eg, fluorescent lamps) generate noise at that frequency.
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I've been grappling with this question for a very long time. There must be a logical reason to explain why almost all humans have one bicuspid mitral valve and 3 tricuspid valves in their hearts.
In the same vein of thought,
  1. Are there any reported cases of people having a tricuspid mitral valve? How would they present in the clinic (if at all)?
  2. Theoretically, what do you think would happen if, during a mitral valve replacement, a prosthetic tricuspid valve was used instead of a bicuspid valve?
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This is an interesting question. 3 leaflets mitral valve is unusual. However, you can have prominent A1 or P1. AV canal can be associated with anomalies of the leaflets number and usually has associated mitral regurgitating.
We can implant tri leaflets biological valve in the mitral position with no hemodynamic effect provided that no LVOT obstruction
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Dear Researchers,
Please, which papers, sites, tools, or programs you recommend to use in the design and selection of the best materials for prosthetic leg liners?
Regards,
Akram
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Dear Dr. Akram Jassim Jawad ,
in addition to the answer of Dr. Abdelkader BOUAZIZ ,
-a Silicone liner provides high stability and good adhesion if your limb has a lot of soft-tissue. ...
-Polyurethane has a unique ability to flow away from high pressure. ...
-copolymer is soft, cushiony and highly elastic, offering good protection for amputees with sensitive skin and/or lower activity levels.
For more details, please see at:
- What materials are used to manufacture prosthetic liners?
- All About Prosthetic Liners: Part 2 Choosing the best material.
Best regards, Pierluigi Traverso.
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Dose any one tell me please which the best material to use for athletics prosthetics for legs under knee joint?
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Carbon fiber composite material which is flexible as well as strong enough to withstand high impact during athletic activities
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I design a 3D prosthetics in SolidWorks and simulate it in Abaqus. When I select section it assumed full prosthetics as a whole part. But I want to select different section manually and assign different mechanical properties. How can I select section independently?
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Create materials, create sections, partition the part, sequentially select partitions and assign desired sections.
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In recent research (pdf attached is below) we found that patients with a low educational level became adapted to the prosthesis less frequently.
This was a cross-sectional study. The patients were identified by primary healthcare teams.
The inclusion criterion was that these should be patients who underwent major lower-limb amputations of any etiology. Associations between sociodemographic and clinical variables and the adaptation to lower-limb prostheses were assessed.
We examined 149 patients. Adaptation to the prosthesis occurred in 40% (60/149) of them, but only 62% (37/60) of these were using it.
Adaptation occurred more often among male patients (P = 0.017) and among those who had a higher educational level (P = 0.013), with a longer time since amputation (P = 0.049) and when the etiology was trauma (P = 0.003).
The result from logistic regression analysis showed that only patients with low education (P = 0.031) were significantly associated with a lower frequency of adaptation to prostheses.
What's your opinion and experience about this?
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I am working at Cancer Center as we do a lot of amputations and I do agree with you, the lower the education level the less frequency of using the prosthesis.
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Hello. I would like to understand the relationship between Rz value and activity of the enzyme. So I have the recombinants of a bifunctional catalase and some of them have really low Rz values but their activity is really high. Since Rz is known as a measure of heme content of the enzyme, not the activity and also its low values indicate that impurity of the protein or insufficient formation of the prosthetic group, how can this be explained if the activity of the variant is really high, in fact, higher than the wild type enzyme itself, but Rz value is really low?
(P.S. SDS PAGE analysis has shown that one of the causers of low Rz value, impurity, is not a factor for the variants)
Thanks,
Gunce
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Hi Gunce Goc,
I would like to place my point of view here regarding the results which you have garnered according to the experiment.
As RZ value directly expresses the purity number/purity of protein (by including ratio of two absorbance readings) with respect to its prosthetic group, but not of an enzyme in terms of its activity. In the early research works, it is reported that divalent metal ions like Calcium ions (Ca2+) maintain the protein structure in the heme environment along with the spin state of heme iron that ultimately promotes the optimum conditions for appropriate enzymatic activity. So, If your RZ value is decreasing, then we can speculate that the cofactor might be having ease in terms of its regulatory effect upon the enzyme activity as comparable to the wild type. Variant enzyme activity also gets increased due to the amino acid substitution or change in the aminoacid positions, not in all cases but if specific amino acid positions are targeted in respective to their way of action, like in recombination.
{There are few things which are not mentioned in the question like how much RZ value of variant enzyme got decreased in comparison to wild type and what kind of activity parameters did you specify in accordance to the standardisation of your recombinant enzyme activity through which you seek such enhanced results}
I hope this will help you to clear some of your doubts.
Thank you,
Regards
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Hello. Hope everyone is fine.
I am wondering if skin bio-prints that can be made in 3D bio-printers have been use as skin grafts and work or if they have only been tested for models of treatment or disease? If yes, what would be the 3D bio-printer for that matter?
Also what would be best for prosthetic in amputees a 3D printer or a 3D bio-printer? And what model not highly expensive would be best?
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Hello Demian!
See this recent skin graft paper that uses the CELLINK BIOX 3D Bioprinter!
“Three Dimensional Bioprinting of a Vascularized and Perfusable Skin Graft Using Human Keratinocytes, Fibroblasts, Pericytes, and Endothelial Cells”
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Is there proof of helping or hurting one's ability to incorporate a prosthetic by use of phantom sensation?
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Yes I can't accept this concept, since the paient may or not accept the pros.from 1st time...
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Thanks for the answers...
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Hi,
What I understood is that an upper-knee amputation post infection + life threatening medical assistance + multiple sequential amputations caused a fat embolism.
I meant that in post-infected ans scared tissues with possible weak veins, it is hazardous to restrict the embolism entry to the shaft marrow since is seems there was no material pushed inside in the bone shaft; such insertions may cause fat embolism.
For upper knee amputation, by definition, we loose a lot of weight, which is changing the remaining thigh balance in the side coronal plane, since the femoral offset is not changed. Too much correction has consequences for sure.
Soft tissues tend to "melt" very quickly. Then we tend not to strip muscles (they will turn into fat) and fat regarding the bevel bone cut. And in fragile tissues, it is important not to have too tight tractions one the skin causes by the stitches. It may cause succion troubles in the prosthesis but it is another problem.
Talking about shoes, a bare foot has a lot of fat pad regarding metatarsian heads and ever stronger shell at the heel (very useful in Syme's amputation).
Cheers, JM.
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Another challenge in making clear decision in this particular case to me. Any thoughts?
A 58 years old completely has no past medical history previously who was physically active prior to approximately 2 months. He was then found to have severe Strep gallolyticus (previously known as bovis) IE involving AV and MV leading to wide open severe symptomatic AR and MV abscess and at the same time non invasive CA colon. What would be the best approach? Valve replacement surgery vs colectomy? What is the risk of recurrent prosthetic valve IE in the setting of CA colon?
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Yeah, I agree but this is such a situation that is difficult to make a precise decision due to its rarity. Hence, one makes a judicious decision based upon the clinical presentation and urgency of the one condition over the other. If the patient can tolerate, one can always go for endoscopic extirpation of the CA-C and it will put our minds to rest for recurrent infection of the valve. Hence the decision must rest finally with you taking into consideration of the clinical setting. Regards,
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I have designed a EMG signal acquisition unit using analog circuit and the system is interfaced with matlab using arduino. Now the problem is that, matlab takes around 3sec for receiving 250ms data send by arduino. it is also confirmed that arduino is working well and sending data at specific sampling rate. Moreover, it is also tested that fscanf function in matlab 17 takes about 1000microseconds for receiving a data which is sent by arduino in 84microseconds. So, how can i minimize the acquisition time to use it for real time prosthetic devices?
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If I were you, I would buffer the data first in the Atmega chip first then transfer the data chunk to the matlab. Lets say buffer every fey bytes of data.
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I am a veterinarian from Austria and we operated on a bearded vulture 1 year ago where a strangulation injury led to the amputation of a foot. She is the first bird worldwide where we were able to prove that osseointegrated prosthetics work for avian patients. since then she is living perfectly with it. for my future cases, I am looking for sensors that I can place on the bird leg to measure the movements of the healthy leg in comparison with the other one. It would also be useful if there are sensors that can also measure which forces apply especially when landing and walking with the prosthetic. that way I might be able to design the external prosthetic in a different shape and from other materials to make it as physiological as possible. If you know someone who can help me with that it would be awesome!
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If you haven't found anything yet, I'd like to suggest using a triple axis accelerometer attached to a battery powered WiFi module. You can attach the module to the bird like a necklace tracker and get real time data from the accelerometer about the birds movements. You'll be able to analyze the movement and tell which leg the bird is on and how the movement differs between legs.
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Hello,
I'm working on developing an idea for a capstone involving prosthetics. My original idea had been a 3D printed sport-specific hand for pediatric patients with limb deficiencies, but since they are so adaptive, the need for it may be low.
I am hoping to stay in the area of pediatrics, and wondering if there are any suggestions on "literature gaps," unanswered questions, or simplistic devices/solutions needed.
Thank you for any suggestions!
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Following answers....
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I am curious to see what other people's views on neural prosthetics are, specifically ethically, and why they think/feel this way.
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Please provide the neural prostheses are interested in.
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Artificial prosthetic hand have a different type and shape of fingers
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Thank you Dr.Jumaa for your question , we think the following link is useful https://dspace.lboro.ac.uk/dspace-jspui/bitstream/2134/2563/3/JayandHavenit HandSizePaper_corrected_.pdf regards
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Hi dear reader,
After loading the prosthetic part, there will be bone loss ( about 1mm) in the first functioning year. This could affect the long term success for short dental implant.
Best wishes
Sarmad
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all we have to do should be done before implant insertion, good selection and good treatment plan but after loading crestal bone loss starts if maintenance of the coronal attached tissue gingival seal has been lost
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Now i am working about prosthetic hand. And classification accuracy is related with subject (dependent or independent).
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Very interesting question, and I have some experience regarding this problem. So far I found very little variation in the data pattern of sEMG signal person to person. The data we find from sEMG signal is not critical in nature. Due to this, you can easily claim it as person dependent. One more thin I should add, sometimes the classification accuracy can be slightly varied person to person. If you need more help, you are welcome brother....
regards!
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There are different shape can be used such as circular ,semicircular ,rectangular,and others
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semi circular finger is good shape becuase it has good contact area
regards
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  • NOACs are currently contraindicated in patients with metallic prosthetic valves. Furthermore, there is no evidence of their benefit in patients with significant valvular Atrial Fibrillation. Will this postion change in the future? Are there ongoing trials in patients with metallic prosthetic valves or high risk valvular AF patients that may drive the addition of these indications to the utility of NOACs,
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Thanks Enrico. It will be interesting to await the results of this study. However, given the open nature of the study and its small size, (50 patients per arm), it is highly unlikely that the study would support a regulatory submision (certainly in major agencies like the FDA and EMA) to expand the use of rivaroxaban in patients with mechanical prosthetic valves.
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Most of the amputees are suffering from the secondary disorders which are more common in upper extremity one. There is more pressure on upper limb amputees’ intact hand since they have to do every single task just by one hand and this imbalance causes musculoskeletal problems such as scoliosis. Despite doctors advice, due to the difficulties of prosthetic hands uses especially in above elbow cases, some of the amputees desist from using it, after a while. I want to know if cosmetic prosthetic really prevents spine deformity like scoliosis?
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I'm looking for the latest developments on implantable artificial lungs either in researches or in patents. I have found very few successful ones and I wonder what the real challenges are.
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Ms. Yousra Hussain Aljazairy
many thanks for you response.
The artificial lung in a backpack is an external device which is also really helpful.
But I'm mostly looking for a "bio 3Dprinted" kind of a prosthetic lung which could replace the need for transplant.
something like heart that is being bio 3Dprinted by experts in that field.
like this one:
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Good afternoon, I am working on a series of prosthetics, one of which is a mammary prosthesis, and my ultimate goal is to have them approximate some of the mechanical properties of fatty tissue.
Originally I was using deadened silicone but was not satisfied with the results and Potting/Medical grade gels are not practical (price/MOQ).
I have been researching hydrogels for years and, being mostly water, believe it could give me close to what I want but I'm stuck, I know of various protocols for preperation but Ideally what I am trying to achieve is a material (a hydrogel) that:
*Wobbles/jiggles
*Deforms under it's own weight
*Is very soft, strong, hyper elastic gel
*Is cohesive, stable with some shape memory
*Can, preferably, be a delayed two part system to injection cast into a mold
*Is able to stick (not bond) aggressively to a silicone encapsulation (difficult I know)
*More importantly, can be safely created, without laboratory equipment or restricted chemical compounds in the UK
Is any of this remotely achievable? I'm thinking along the lines of blending natural/synthetic polymers, stabilisers and crosslinking but I can't find any research on something like this.
Please ask any questions if you would like more information.
Thank you in advance
*N.B. I am learning/studying all this Chemistry, Physics and Maths as I go along so apologies if I don't understand right away.
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Thank you very much for this, numbers 4-6 specifically caught my eye. Number 4 especially (...Adipose-Tissue-Inspired Viscoelastic Composite for Tunable Soft Implant and Elastomer).
I will read them all this weekend and see if anything stands out that I could discuss here.
Thanks for all the help from you both.
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My research focuses on architecture as a device, controlling and transforming the bodies.
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Dear Mauro,
Thank you for your observation. I did try them again and the sites were closed. I have sought other materials that maybe useful. However, I have noted that the articles I have read deal mainly with the elderly/seniors, as such you study promises new insights. I do understand that the design needs for women are slightly different and should be considered.
Best wishes.
Evelyn
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prosthetic lower limb contains
socket
pylon
foot
Knee
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Thanks for your response
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Prosthetic limb consist of
socket
pylon
foot
knee(without knee for below knee amputee and with knee for above knee amputee)
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Thanks for your answer Mr. Roland Auberger
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As a general rule, the absent tooth will be the most distal tooth of any given tooth type . Lower premolars are the most frequently absent teeth with a prevalence of
2.6% (mainly symmetrical) followed by missing upper lateral incisors at 2% (more frequently bilateral than unilateral). The treatment options for missing upper lateral
incisors usually include space closure with canine substitution or space opening with subsequent restorative replacement. Only on rare occasions are patients either happy to accept the aesthetics of the missing lateral incisor, or is the space ideal for a direct prosthetic tooth or canine build up.So, When are Space Opening and Space Closure Appropriate?

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When it is the lower second premolar (CML5) and the patient is young, the case can be closed easily - without losing too much anchorage - by using Hemisection. (Northway, W. Hemisection: one large step toward management of congenitally missing lower second premolars. Angle Orthod 2004; 74: 790-7.)
When the tooth is in the esthetic zone, for example the maxillary lateral incisor (CMU2) (Rosa M, Lucchi P, Ferrari S, Zachrisson B, Caprioglio A. Congenitally missing maxillary lateral incisors: Long-term periodontal and functional evaluation after orthodontic space closure with first premolar intrusion and canine extrusion. Am J Orthod Dentofacial Orthop 2016;149:339-48), space closure is the preferred method of treatment. In conclusion, age and esthetics dictate treatment propriety.
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we use semi-adjustable articulators for full mouth rehabilitations. taking protrusive records to program articulators can sometimes be cumbersome and time consuming.
average values for different age groups and genders can reduce precious chair side time
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Ask Tom Lee at Panadent. His company sells an articulator with curvilinear analogs based on statistical averages. He may be able to recommend analogs having the "values" you seek. If you are using adjustable mechanical articulators, checkbites in working and balancing sides during intermediate right and left lateral positions may be helpful.
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I want to classify multi class sEMG signal for designing a prosthetic hand.
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I need soft copy of this book " Machine Learning with R Cookbook " by Yu-Wei Chiu"
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I want to classify EMG signals for designing a prosthetic hand, so i need to extract unique feature of each class.
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Vai Tusher,
Assalamualaikum. This discrete windowing is very simple method. It means how many samples of EMG time domain or Frequency domain would you like to consider for taking decision. Is you need help in detail, you can contact with me directly. my email ID: bmeasadur@gmail.com. All the best.
Regards
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Prosthetics in running.
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Lower extremity leg amputation: an advantage in running?
Knut Lechler and Magnus Lilja
This paper is a neat review.
Also,
Counterpoint: Artificial legs do not make artificially fast running speeds possible.
Kram et al
Hope this helps
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should we wait for a while or we could receive a temporary prosthesis immediately following amputation? What items influence on this timing?
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You can be molded and fitted as soon as the skin is healed and the edema has subsided. I try to get these on and begin rehab ASAP
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On a prosthetic point it is a really good ongoing point of quality of life. Idiocratic ironically is a advanced point of research Qualitative. Environmental can be cyclic theory or stages of ?
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Relationship Change Between Couples Perspectives from Different Countries
Please let me know if these references/sites are helpful to you:
1.  Trajectories of Couple Relationship Quality after Childbirth: Does ...
by MJ Carlson - ‎2007 - ‎Cited by 26 - ‎Related articles
among low-income and unwed couples with children can facilitate the ... examine the level and change in couple relationship quality subsequent to births in ...
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Jul 3, 2014 - relationship quality than do married individuals (e.g., Brown & Booth, ... terrain of U.S. families by elucidating the changing roles of cohabitation and marriage in the ......
3.  Understanding Couples' Relationship Quality ... - Guttmacher Institute
regression analyses to identify associations between relationship quality and current contraceptive use among 698 
Dennis
Dennis Mazur
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In leg amputation the remained bone is rasped to make the edges smoother to avoid tissue injuries. but still there is an unusual structure underneath the skin. 
Why don’t we use an implantable structure to avoid abnormal structure like sharp edges and damp the undue pressure and shocks underneath bones, the same way heel fat pad*(HFP) protects the underlying structures in the heel?
From my point of view, using such implants might also ease the use of prosthetic legs and decrease the possible pain in leg- prosthetic interfaces.
Are there any specific reasons not to use such implants during the leg amputation?
*The heel fat pad (HFP) is a highly specialized adipose-based structure that protects the rear foot and the lower extremities from the stress generated during the heel-strike and the initial support phase of locomotion. HFP cushioning efficiency is the result of its structure, shape and thickness.
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Bevelled bone end + adequate thickness of myoplasty should do the trick. Implant on the bone end is not necessary especially if the prosthesis is not designed to receive weight transmission through the end stump. If we worry about bad soft tissue surrounding the bone end, putting an implant on that area will create more problems, such as rapid implant wear, infections. Higher amputation level with good soft tissue should be put into consideration.
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With regard to lower limb exoskeleton, some of its purposes related to patient with walking difficulties are gait restoration and gait rehabilitation. Just found out that gait restoration and rehabilitation are different. Can somebody give brief explanation on the differences between both?
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Good. This will depend on the injury / residual capacity of the patient. It is possible to walk without support (rehabilitation of walking). If you need some support (bracing, prosthesis, walkers, crutches, etc) we would say that it will need recovery "with support". That is, you can walk again, but you will always need support.
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What is the protocol recommended for the zirconia restorations? 
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The problem that you have is that zirconium does not etch. Even HF acid will not etch the surface of this material. If you micro abrade the material it will initiate surface damage that could result in cracking. I have had submissions to IJOMI the talk about aluminum oxide abrasion, different bonding agents, and even leaving the service alone as it was received from the manufacturer. Depending upon the study setting and specific nuances relative to the way that the material was handled different studies will show completely different results. I think if you plan on seeing the bonding to the zirconia surface that's probably the correct answer. Indeed a thin layer of low using  porcelain  will allow etching with and how does that affect the fit of the restoration.
Unfortunately zirconia is a tricky material and because it is so tricky every different technique in different hands may well act slightly differently.
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Can some one suggest to me what the mechanism i have to choose for movement robotic hand part.
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by using electrode sensor, it is putting on muscles and it collect the pulse then amplifier to motor of prosthetic hand  
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Do any one has experience about the ACL graft fixation on the tibial side with cortical devices like the Endotack of Storz or similar devices that a tunnel is drilled rather than a socket like that performed with the All inside Graft link of Arthrex. Are their any disadvantages that prevents their popular use like the Interference screws?
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Thanks a lot .. for the answer Dr Kuik
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Spinal engagement is common. Why not prosthetic infection in patients with Brucellosis? To my knowledge surgeons do not take into consideration this specific infection.
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Thank you, Dr Malyk. This was my idea. When diagnosed in time to have the opportunity to save the prosthesis by adequate antibiotics.
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When I run an SDS of my Ni-aff purified protein I get 2 bands, one at approximately the correct mass and one 2kDa higher. I confirmed these masses by MS and also ran an assay where I attach a prosthetic group to the protein. This results in both the proteins increasing by the expected mass, suggesting they are both ACPs.
I've tried different strains of E.coli, gel filtration (but the masses are too close), using a cobalt column instead of nickel, running the Ni-aff on an FPLC with a gradient and can't overcome this problem. Another member of my lab has come across the same problem, but hasn't managed to solve it.
Can anyone suggest ways this could be resolved? I've seen suggestions about changing the loading buffer of the SDS-PAGE gel, but this wouldn't get rid of this second band.
Thanks.
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You may be looking at posttranslational modification of a single protein. Since you have access to MS, I'd try to use that for sequencing. Ideally, you should be able to identify the modified amino acid and the nature of the modification.
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I have been unsuccessful at contacting them directly.
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Oddgeir Friborg is on ResearchGate. He has posted the full text of his thesis here. It is a complete report on the development of the scale. It should contain scoring information. But even if it doesn't, you can contact him via ResearchGate.
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Hello,
I am looking for a system to monitor pistoning of residual limb in a prosthetic socket. There is an approach [1] which relies on motion capture system, but their results are for static monitoring along. Did anyone try this approach in walking trials? Or Sanders's approach [2] is the only choice?
Thank you.
[1]Gholizadeh H, Abu Osman NA, Kamyab M, Eshraghi A, Wan Abas WAB, Azam MN. Transtibial prosthetic socket pistoning: static evaluation of Seal-In® X5 and Dermo® Liner using motion analysis system,
[2]Sanders J, Karchin A, Fergason J, Sorenson E. A noncontact sensor for measurement of distal residual-limb position during walking.
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Thank you for the kind help.
Sincerely,
Ming
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I need to have a prosthetic heart valve modeling
You can now give to me this model will be ready in Catia or Solidworks ?
There is a illustration in the Annex
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Hi Mr Bruno senzio
Thanks for the replies.
No,I want a bioprothetic heart valve that I attached Top Comment.
Your image is mechanical heart valve.
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As far as I know with my limited knowledge, use of dental implants contraindicated in children of growing age, is there any change in the concept? is there any advance in Oral implantology to enable use of dental implants in such children? especially in children with ectodermal dysplasia or partial/complete anodontia?
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OVER THE PAST 30+ YEARS we have observed that Branemark system implants placed in your patients will stay static in spacial position as the cranium continues to develop and grow.  Infact, this growth continues through out  adult life as well.  it is especially relevant in the maxilla which continues to grow downward and forward.
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We are looking for scientific papers and/or reports ( both Government and Non-Government) which looks at the effectiveness and cost effectiveness of Prosthetic and Orthotic Services. 
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too many variables to get results within the margin of error.
Consider foott yping the cohort to cull the  subjects that would reduce variables.
Dennis
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Miguel Constantino and his group were examined the cytotoxicity of old material which is Acrylic reline resins by using salivary acetylcholinesterase as substrate, therefore I would like to examine the cytotoxicity of a new material (polyamide) and I'm searching about enzyme substrate to do my work. Thank you 
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Dear Ali,
Since your compound is an amide, I suggest that you test its cytotoxicity using amidase enzymes.
The following papers might serve as a starting point for your experiments.
1- ACS Chem Biol. 2015 Aug 21;10(8):1838-46. doi: 10.1021/acschembio.5b00114. Epub 2015 Apr 15.
A Potent Systemically Active N-Acylethanolamine Acid Amidase Inhibitor that Suppresses Inflammation and Human Macrophage Activation.
Ribeiro A1, Pontis S1, Mengatto L1, Armirotti A1, Chiurchiù V2, Capurro V1, Fiasella A1, Nuzzi A1, Romeo E1, Moreno-Sanz G3, Maccarrone M3,4, Reggiani A1,Tarzia G5, Mor M6, Bertozzi F1, Bandiera T1, Piomelli D1,7.
Author information
 
Abstract
Fatty acid ethanolamides such as palmitoylethanolamide (PEA) and oleoylethanolamide (OEA) are lipid-derived mediators that potently inhibit pain and inflammation by ligating type-α peroxisome proliferator-activated receptors (PPAR-α). These bioactive substances are preferentially degraded by the cysteine hydrolase, N-acylethanolamine acid amidase (NAAA), which is highly expressed in macrophages. Here, we describe a new class of β-lactam derivatives that are potent, selective, and systemically active inhibitors of intracellular NAAA activity. The prototype of this class deactivates NAAA by covalently binding the enzyme's catalytic cysteine and exerts profound anti-inflammatory effects in both mouse models and human macrophages. This agent may be used to probe the functions of NAAA in health and disease and as a starting point to discover better anti-inflammatory drugs.
PMID:
25874594
2- ArticleBiotechnology Letters
March 1984, Volume 6, Issue 3, pp 149-154
A study of the inhibition of an amidase with a wide substrate spectrum and its consequences for the bioconversion of nitriles 
M. Maestracci , K. Bui , A. Thiéry , A. Arnaud , P. Galzy
Summary
TheBrevibacterium sp. R 312 strain possesses a nitrile-hydratase and an amidase, both with a wide substrate spectrum. These two enzymes can be used for the bioconversion of nitriles into the corresponding organic acids: the actions of three types of compounds (nitriles, amides and acids) on the activity of the amidase are reported in the present work.
3- A Novel Mechanism Underlies the Hepatotoxicity of Pyrazinamide
Tung-Yuan Shiha,
Chien-Yi Paib,
Ping Yangb,
Wen-Liang Changb,
Ning-Chi Wangc and
Oliver Yoa-Pu Hua,b
+Author Affiliations
aGraduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan
bSchool of Pharmacy, National Defense Medical Center, Taipei, Taiwan
cDivision of Infectious Disease and Tropical Medicine, Tri-Service General Hospital, Taipei, Taiwan
 
Next Section
ABSTRACT
Relatively little is known about the hepatotoxicity of pyrazinamide (PZA). PZA requires activation by amidase to form pyrazinoic acid (PA). Xanthine oxidase then hydroxylates PA to form 5-hydroxypyrazinoic acid (5-OH-PA). PZA can also be directly oxidized to form 5-OH-PZA. Before this study, it was unclear which metabolic pathway or PZA metabolites led to hepatotoxicity. This study determines whether PZA metabolites are responsible for PZA-induced hepatotoxicity. PZA metabolites were identified and cytotoxicity in HepG2 cells was assessed. Potential PZA and PA hepatotoxicity was then tested in rats. Urine specimens were collected from 153 tuberculosis (TB) patients, and the results were evaluated to confirm whether a correlation existed between PZA metabolite concentrations and hepatotoxicity. This led to the hypothesis that coadministration of amidase inhibitor (bis-p-nitrophenyl phosphate [BNPP]) decreases or prevents PZA- and PZA metabolite-induced hepatotoxicity in rats. PA and 5-OH-PA are more toxic than PZA. Electron microscopy showed that PZA and PA treatment of rats significantly increases aspartate transaminase (AST) and alanine aminotransferase (ALT) activity and galactose single-point (GSP) levels (P < 0.005). PA and 5-OH-PA levels are also significantly correlated with hepatotoxicity in the urine of TB patients (P < 0.005). Amidase inhibitor, BNPP, decreases PZA-induced, but not PA-induced, hepatotoxicity. This is the first report of a cell line, animal, and clinical trial confirming that the metabolite 5-OH-PA is responsible for PZA-induced hepatotoxicity.
Hoping this will be helpful,
Rafik
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During a presentation on prosthetics coordination through non-invasive BCI with EEG, it was shown that patients were able to do so through focusing on the imagined movements of their phantom limb. I'm wondering if this is more effective on patients who have had control over the corresponding limb where the motoric pathways are "familiarized" with the tasks compared to patients who are missing that limb from birth.
I'd appreciate any information/guidance on the subject!
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Dear Zeynep,
That is a very good question, which to the best of my knowledge is pretty unexplored territory. The issue with using BCI/BMI for patients with a congenital limb deficiency is that they will not have formed the appropriate neural pathways to control the limb - thus there is no chunk of the brain which is soley associated with controlling the missing limb. 
That does not mean that alternate control strategies cannot be used, though.
Now, based on the very good results that the cochlear implants have had when implanted at a very young age (showing better results than adult implants), I would expect that a BMI when used consistently from a young age would give much better results. The issue with that, though, is that the child may not accept the BMI or prosthesis.
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Bench alignment measures could be done by PROS.A. Assembly or L.A.S.A.R Assembly from Ottobock. but I still don't know how to the find the socket axis and do these measurements:
socket AP shift
socket AP tilt
socket ML tilt
socket rotation
Could someone explain how can we measure these parameters and define the socket axis
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Spine Mouse device is useful for measurement of all joints too. It can be possible for prothesis but you must determinate exactly the axis of prothesis or standarised  it.
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can someone give the opinion and answer this question, what the material or equipment are suitable as a mechanism for movement of the prosthetic hand robotic parts?
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I agree with mister Hosseini. It is impossible to give general suggestions. It is possible to realize lightweight constructions with steel. In other cases aluminium, titan or CFRP are the best suitable material. For low cost structures I would buy the MarkOne printer. Think this covers a wide range of applications but still depends on the design and constraints.
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One of the commonly performed minimal access procedure is Lap mesh ventral hernia repair.we started with plain polypropylene grafts and now we are using composite mesh grafts.The market is flooded with variety of expensive grafts.Most of them are good. It is always a difficult task to choose.One such is symbotex which has been recently introduced in India.
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I have no experience in their use
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The knowledge of the clinical performance of flexible polyamide/nylon prostheses is very limited. There are several questions about their lower impact and flexural strength, fatigue resistance, and polymerization shrinkage.
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I totally agree with the answers. Thank you Murali Srinivasan and Mohammad Ayahm Kakkoum.
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Hi, 
Is there a book(s) do you use or recommend about this topic? I have read some of the "Biomechanics of Lower Limb Prosthetics" from Pitkin and it has some design concepts, but I would like to check others.
Thanks in advance, I would appreciate any suggestions.
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Dr. Vargas,
This atlas may be useful for you. 
Atlas of limb prosthetics: surgical, prosthetic, and rehabilitation principles
John H. Bowker, American Academy of Orthopaedic Surgeons
Mosby Year Book, 1992 
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As a result of extended tumor resection, tension-free abdominal wall closure sometimes is not possible anymore.
Which prostethic mesh is going to work best? In my experince, wound infection is a major issue here.
I would be happy to share my thoughts with collegues also working in this field.
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If I cannot close an abdomen in a child immediately, I use a synthetic patch such as goretex in combination with negative pressure wound therapy with Acticoat as the interface, this can be left easily for a week. The abdomen can be then closed after removing the patch several days later. If I want something more permanent then I use Surgisis for the fascial defect.
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I am writing my bachelor thesis about participation assessments with people who have had a lower limb amputation. Now I have to evaluate which of the instruments I used, offer me the most information about the client..
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You are welcome ;)
good luck
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Various devices used by the disabled are being explored and the user analysis is planned, further i request suggestions for enrichment
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Dear UV Kiran,
a good starting point could be the European Directive EN 301 549 V1.1.1 (2014-02) which is open and publicly available at ETSI website (http://www.etsi.org/technologies-clusters/technologies/human-factors?tab=2). There you will find also related standards which may be of interest too.
Hope this helps,
Mireia
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I would like to see where orthopaedic community stands with diagnosis of PJI and according to surgeons, what is the best diagnostic test when it comes to PJI?
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Dear Dr Shahi,
You can find a new marker in the paper with the link below:
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I have a small sample size. I read that for a small sample size LPP can be used
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OFNDA is one method, since myo signals are small in samples, and LPP is used in 2D image analysis  such as face recognition or palm print whch too have small sample size, pl let me know whether LPP can be implemented for  one dimensional myo signals
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I have a pediatric patient  (8Kg) currently on 9mg of warfarinfor a prosthetic valve in mitral position. She has an INR of 1.3
The accuracy of preparation and administration of medication has been thoroughly checked. She is not on Vit K supplements. She has no malabsorption or other related concerns. She has not been tested for warfarin-resistance polymorphism, VKORC1 Asp36Tyr as this is not easily available. IV warfarin is no longer available in the US and hence cannot test oral absorption related problems with an IV administration. 
My questions are:
1. Would you use as high a dose as it takes to get the INR in the therapeutic range?
2. Is there a side effect unrelated to coagulation that you would be concerned about ?
3. Would any one have similar patient in pediatric age group to do a focused evaluation to understand warfarin resistance? 
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There are no other available effective/proven oral anticoagulants for prosthetic mitral valves. Warfarin resistance is well established. There are established cases in adults who required 145 mg/day to maintain a therapeutic prothrombin time (MacLaren R, Wachsman BA, Swift DK, Kuhl DA. Warfarin resistance associated with intravenous lipid administration: discussion of propofol and review of the literature. Pharmacotherapy 1997; 17:1331–1337) I am looking for pediatric experience and discussion.
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I am searching about a database or register where significant aorta valve data had recorded? Interesting the mortality time and echo parametric values for those patient which waits for prosthetic valve implantation or TAVI. Thank you
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UK TAVI database
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I need some tissues that have analyzed prosthetic hands dynamically, or some investigates in force systems of this prosthesis.
Could anyone help me please?
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thank you for your recommendation Mr.Schorsch.
i will chek that.
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Patient is currently under antibiotic therapy for prosthetic (bovine) valve endocarditis, CRP is almost negative, there are no vegetations and no insufficiency of the valve. Therapy will last for two more weeks. Knee replacement surgery is scheduled in four weeks. Should we wait longer? How Long?
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In any case - the prognosis of "prosthetic" endocarditis of the bioprosthetic heart valve  is  poor  and any surgical procedure may provoke him.
However, let me know what was the basis for the diagnosis of "prosthetic" endocarditis?
Did You based on any "large" criteria (obviously, one of the "big" criteria absent - no vegetations on the valve), or on a set of "small" criteria? What exactly?
Have there been attempts to detect other foci of infection, in addition to the valve implant?
Has a procalcitonin test?
If we are talking about an infectious lesion of the bioprosthesis and successful antibiotic treatment, I would advise you to delay implantation of the knee up to 6 months.
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The current pattern recognition-based EMG control is highly patter-motion related. Once a maping of EMG patterns to desired motions is predefined, it is hard to re-configure and apply again. Considering the human hand grasp is controlled by C.N.S. in a coordinated way (synergy), rather than a one-to-one specific way, I am wondering if this finger coordination can be found in the EMG signals and thus through suitable algorithms these information can be extracted and applied to the prosthetic hand's grasp control.
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Oh, Castellini has given the answer. "Evidence of muscle synergies during human grasping," Biol Cybern, vol. 107, pp. 233-45, Apr 2013.
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What is your approach to cellulitis and erythema  over arterial side of prosthetic AV graft for dialysis without purulent discharge after 2 weeks of surgery? Antibiotic or graft excision?
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Does the patient exhibit any other signs of infection? Also is there either an elevated white count, or CRP level?
Differentiating between an inflammatory reaction to the PTFE graft versus infection can be tricky. If all you have is erythema in the absence of any systemic markers then I suggest you give a week of antibiotics and continue to observe the situation.
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The FGP technique is not always the panacea. We are practicing different approaches for the last 20 + years and I can assure you that:
1. You will need a very distinct indication.
2. One registration approach does not fit all indications.
3. You need a very detailled understanding of occlusion.
4. Translation into prosthodontic work does not work with all materials and techniques.
5. You need an excellent lab technician.
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The control of robotic prosthesis via signals from the nervous system has reached an impressive level in recent years. However, the feedback of signals from the prosthesis back into the nervous system, e.g., to provide touch sensation, remains a much more challenging problem. Could methods of optogenetics provide an alternative interface in this context?
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Dear Alejandro,
Yes it could: in fact there's an application to restore vision in some forms of blindness, mostly retinitis pigmentosa (progressive degeneration of photoreceptors) using virally delivered channelrhodopsin combined with special goggles. Papers from mice have already been published (from Botond Roska and others), and I heard a recent talk that showed very promising preliminary results pointing towards human applications - so I am very optimistic :-) Here are some links to papers and news pages:
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It is usually recommended to use a tissue heart valve in ladies in child bearing age, requiring a heart valve replacement, to avoid the complications of anticoagulation during pregnancy. But prosthetic tissue valves get degenerated in a few years, much faster than in older patients, in patients of this age group. These patients may need a repeat surgery for their tissue valve before they complete their family. Thus the purpose of implanting a tissue valve may not be achieved.
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All depends on the valve that has to be replaced. Possible pregnacy and young age related to valve longevity ( and expectd growth? (often not a major issue in a teenage girl?)) are the main predominators of the prosthesis' choice in a young girl. Aortic valve: Ross operation with a bioprosthesis (homograft or allograft) in pulmonic position which could be replaced later in life by a percutaneous pulmonic valve when necessary. This will avoid metallic valve related thrombotic and fetal problems during pregnancy and percutaneous replacement of the pulmonic valve seems to be a reliable alternative for surgery. Moreover, the neo-aortic valve (pulmonic autograft) seems to grow up to adult size and has a excellent longevity. Pulmonic valve: by preference a bioprosthesis, percutaneous or surgical depending on the anatomy and size of the RVOT. Anyhow, metallic valves are performing worse in pulmonic position (increased thrombosis rate). For the tricuspid valve, a conservative approach (repair and ring) or bioprosthesis seems reasonable, since degeneration takes place in a low pressure system and even percutaneous replacement of the tricuspid bioprosthesis in the future might become a real option (case reports and small series already available). For the mitral valve, I would prefer a metallic valve if replacement is really necessary, since the fate of a bioprosthesis in this position is really unpredictable. Of course, all depends on the local health care system, resources and follow-up issues.
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I am looking for standardized methods for quality evaluation of dentures. If possible please share references to articles where denture quality was assessed.
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Unfortunately there is no such thing as a standardized method though Fenlon et al (2002) and Sato et al (1998, 2000) have suggested fairly comprehensive methods.
If you do a search of the literature you will find dozens of articles which have attempted to correlate a clinical assessment of denture quality with patient satisfaction. Not one has found such a correlation, hence the emphasis these days in research articles is to assess outcomes in terms of patient satisfaction, and the method mostly used for that is to use VAS scores. OHIP and other socio-dental indicators show great variance within individuals (and even the time of day) so their reliability is questionable. They can, though still be useful as an indicator (but not a correlate) of denture success.
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We observed that the prevalence of adaptation to lower limb prostheses was 38%. And patients with a low level of education were the least frequently adapted to the prosthesis.
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I found this article interesting. It was surprising that a delay in fitting led to better results and that marital status had apparently no impact.
Unfortunately there is too often a lack of adequate care on the part of prosthetists for various reasons of training, supervision, job pressures, and unfortunately attitude. Prosthetics is a small, specialized field that has been allowed to deviate from general standards of care that were once considered essential. Often physical therapists are not adequately educated or experienced in amputee care to evaluate the nature of what can be fairly subtle problems. Frequently I have seen otherwise well-educated and well-meaning therapists try to evaluate the correctness of prosthetic limb length with the patient lying down.Likewise few physicians have the background to sort out the details of amputee care and fitting. There is a huge gap that can only be filled by having experienced , dedicated, and knowledgable people from the various disciplines working as a team to insure that critical aspects are covered. The role of the amputee clinic is essential. The majority of amputations are more and more due to systemic illnesses that are expensive to treat but can be ameliorated by the kind of follow-up care such clinics can provide.
Education level generally reflects something about financial resources and the ability to adapt. The poorer a person is or the fewer resources they can muster the less likely they are to have a better outcome. Less education and poverty are always obstacles and lead to accepting outcomes that seem inevitable but can be greatly improved. This happens more easily when a person is not adequately informed ,is handed along without proper oversight, and is not educated enough to know or expect anything different. The complexity of medical care and appointments and medications and funding can easily become an endless litany that is impossible to keep track of and imposes its own burdens and negative outcomes. This kind of poor,fragmented care is the final breaking point in the goal of independence.
Thank you Dr Nunes for a well researched timely article.
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