Prostate - Science topic
Prostate is a gland in males that surrounds the neck of the URINARY BLADDER and the URETHRA. It secretes a substance that liquefies coagulated semen. It is situated in the pelvic cavity behind the lower part of the PUBIC SYMPHYSIS, above the deep layer of the triangular ligament, and rests upon the RECTUM.
Questions related to Prostate
I am trying to run WB to detect full-length AR and AR-V7 in sc xenograft tumor tissue generated from 22Rv1 human prostate cancer cells (implanted in mice). I've been using Santa Cruz AR (441) antibodies, but the results have been fairly poor. There is a lot of background and non-specific binding, and the desired bands are not very sharp. Band intensities of the same samples vary considerably from one experiment to the next, in spite of a fairly uniform and consistent loading control (B-actin). Would anyone know of a good quality AR antibody for this application? Or has anyone used AR (441) successfully in xenograft protein samples? Incidentally, I have used this same antibody for IHC in these tissues, and I have had good results.
I want to buy a primary anti-PSMA (prostate specific membrane antigen) antibody for western blot and immunofluorescence purposes. I am considering to acquire one of these three:
- Abcam ref: ab76104 clone: EP3253
- Cell Signaling Technology ref: 12702 clone: D4S1F
- Invitrogen ref: 37-3900 clone: 1H8H5
Have any of you used any of these antibodies? If so, I would really appreciate some feedback :)
Or if you use a different anti-PSMA antibody besides these and can recommend it please let me know as well!
Thank you so much,
Our lab protocol was adapted to a Santa Cruz antibody (sc-816) that worked perfectly in mouse prostate, but it has been discontinued. I've since tested a few others that didn't work. I'd like suggestions for androgen receptor antibodies that could be used for WB and IHC in rat and mouse tissue.
I have read that you should fix from 6-24 hours, but since the prostates are so small I'm considering 6-8 hours. Do you think that will be sufficient?
I injected the prostate cancer cells in mouse dorsal prostate gland.
For tumor measurement, I used ultrasonography (vevo2100 model), but it's hard to recognize where tumor is.
I dissected one of the mice, and I confirm the tumor growth.
Can you recognize which one is spine, kidneys, seminal vesicles or something?
The region which I detected is pelvis on supine pose.
I am developing a research project that will use 3D printed models in health education. I'm looking for 3D models of the prostate, pelvis and or male bladder.
Enlarged prostate also known as benign prostatic hyperplasia, or BPH, is a common medical condition. Prostate gland continues to grow...Read More
I used the SMARCD3 Antibody from Proteintech (12838-1-AP) on FFPE material (prostate), and observed a strong brown background all over the glass slides , right after the application of AEC.
Using the same staining conditions (AR: pH 9, 10 min 3% H2O2 digestion, AB diluent with 0,5% TritonX, 1% FCS in PBS, incubation o/n on 4°C, UltraVision LP AEC detection system from Thermo fisher, BIOTIN FREE!!) I had no problems with SMARCD1 and SMARCD2 antibodies on the same slides.
Have you ever seen something similar?
All the best, and thanks for help!!!
Has anyone try to culture the normal prostate RWPE-1 cell line in RPMI medium supplemented with foetal bovine serum? There's some articles available but neither describe details about the adaptation process,
I would need an adaptation protocol or some technicals advices,
Thanks in advance,
I am trying to purify recombinant Prostatic Acid Phosphatase that has a His-6 tag at the C-terminus from HEK293T media for downstream functional assays. I have tried using a HisPur cobalt purification kit, but the purified protein no longer displays phosphatase activity after purification. Is there any way to purify this protein from HEK293T cell media that keeps it in a functional dimer conformation (this is essential for protein function)?
I need some specific cancer tissue samples' slides for IHC which is resistant and sensitive to specific cancer drugs, especially for prostate, lung, and breast cancer. Is it possible to buy or collect from some organization? If anyone has the information of proper source as well as if anyone has some sample, I would like to request you all to help me with this issue.
Thanks in advance.
I am trying to choose the appropriate statistical test to evaluate cells (drug vs control) over 4 different time points (inc. 0hr). Specifically I was testing prostate cells treated with a drug and evaluating the %area of lipid droplet size. I have acquired a % area of the lipid droplet for approximately 60 cells at 0hr (no treatment), 15hr (drug vs control), 24hr (drug vs control), 48hr (drug vs control) and calculated mean change in lipid droplet size at each timepoint. Ultimately I would like to show the significance of drug vs lipid droplet size against time. Advice is much appreciated and a thanks in advance.
Over the past ten years my centre has been collecting data regarding our management of patients following transurethral resection of the prostate. It is common practice to place a three-way catheter and run bladder irrigation post-op, however my centre does not do this and we believe our method streamlines the post-operative course and patients can be discharged quicker.
We are writing up our results and my understanding is that as we have not arranged this as a RCT that it will be difficult to prove the superiority of our method. However I am hoping to say that our method is as safe as common practice measured by multiple variables.
We have collected data for multiple points such as duration of admission, complication rates, re-admission rates, transfusion rates, success of removing catheters etc. To make a comparison between our novel method and common practice I have analysed papers included in NICE guidance regarding prostate resections - all of which would have used the common method. I have attached a table that I have filled in with available data.
I'm afraid I am quite lost as to how to analyse this table - my assumption would be that I should analyse each variable independently between the papers with a nul hypothesis being our method is inferior to common practice and a hypothesis that it is non-inferior. My research has suggested maybe an ANOVA test would be appropriate but again my understanding of this is severely limited - any help would be greatly appreciated!
I need a public benchmark MRI Prostate dataset for image registration/fusion. The dataset should be segmented with multiple segments per image i.e
I can find no references to this in the English medical literature or in my institutional surgical specimen or post-mortem path reports.
the Motic program ( or another program according to your advice ) used for a photomicroscope image analysis for measuring the folds of seminal vesicles and the prostate.
I am a beginner in the research field and a resident in urology and I would like to try a journal that will be appropriate for me to try for the first time, publishing a case report about targeted prostate biopsy with micro-ultrasound.
I'm looking for a program that will do automatic approximate image segmentation for prostate T2 MRI images.
The program should be:
- Automatic without the need for user supplied landmarks
- Robust in the sense of always producing an answer in a reasonable timeframe
- Reliable in that it will always approximately outline the prostate
- Freely available
The program does not need to be:
- Accurate. I only need an approximate answer, accuracy is not as important as reliability
- Open source
Sample Prostate MRI Image with segmentation attached
We would like to evaluate a blood testing device for serial monitoring (ideally 3+ times in different cancer stages per patient). We couldn't find a blood bank that stores a series of samples for prostate and colorectal cancers... Is there any?
Prostate biopsy specimen should be put in a formalin solution (10% NBF) as soon as possible after sampling. However, if formalin use is not preferred within the operating room, can saline be used as a holding solution until all samples are taken (6/8/10/12 depending on the systematic biopsy scheme). Some, rather less verifiable sources, state that there will be a strict time limit on holding the specimen in saline until they really have to be placed in a formalin solution. However i cannot really verify this statement and cannot really find an explanation whether / why it is or is not allowed.
I am working with prostate cancer cells, i want to know who is presently working with PCa cell lines in viro and in vivo in kolkata.any leeds will of great help.
Hello, dear community,
I am currently researching semantic segmentation of the prostate in transabdominal ultrasound recordings.
My current data are insufficient, so I am wondering, whether there are any available transabdominal ultrasound image sets of the prosteate in adult male patients.
I thank you for your input,
We know that prostate secretes various vitamins and proteins including prostatic acid phosphatase into seminal plasma.
But I was wondering if some of these ( particularly prostatic acid phosphatase) is secreted into/comes in contact with blood.
some authors suggested the "anteroposterior approach" rather than retrograde approach
I assume that anteroposterior approach is a bit challenging in a large prostate as the plane between the adenoma and the capsule is easier to find posteriorly, where the major bulk of the adenoma is located, than anteriorly?!!
Carcinoma Prostate has been risk stratified and management based on the same is well established.
However, clinically T3a disease still remains a "grey zone" in the management strategies, in order to achieve the "Prostatic trifecta"".
With emerging radiological evidence that long capsular contact as well as capsular bulge (on MRI) should be considered as clinically T3a disease, the T2 disease paradigm also has had a dramatic shift.
What should be the ideal treatment strategy- Radical Prostatectomy or Radical Radiotherapy?
I am doing work on prostate cancer cell line - LNCaP - and using PSA antibody to show androgen deprivation. We currently have 3 in our lab, none of which are giving us a signal for western blots. They have worked previously for someone else in the lab - all that being said not very well. We have the Santa Cruz (A67-B/E3): sc-7316 and 2 Abcam 1 mouse monoclonal (A67-B/E3 ab2218) and 1 rabbit monoclonal (EP1588Y ab76113). All are stored at 4C as stated on data sheet and have been used as concentrated as 1.100 with no signal. I even tried a native blot which again no signal was observed. Any help would be greatly appreciated.
just a quick question about experience in reading the prostate MRI: is there any minimum number of prostate MRI/year to define the experience of the reader? (i.e: 50 prostate MRY/year = low experience Vs 200 prost MRI/year = high experience).
If yes, is there a consensus about that? Is there any reference in literature?
We are trying to establish subcutaneous tumor in NSG mice using prostate cancer cell line PC3 (engineered with firefly luc). We use IVIS to monitor tumor growth and noticed that the tumor size (Fluc intensity) was largest on Day3, decreased half on Day7, and slowly increased in the following 2-3 weeks, but still not reaching or surpassing the reading on Day3. We worked with subcutaneous tumor model in NSG mice before using other cell lines (eg, lymphoma) and we have always observed very fast and exponential tumor growth.
So I would like to ask;
1. Is what we observed with PC3 cells normal?
2. Would other prostate cancer cell lines (Lncap, Du154) form better subcutaneous tumors in NSG mice?
3. Are there other ways to improve PC3 tumor growth in vivo? (We noticed that the PC3 tumors grow better with matrigel, but we would like to avoid matrigel usage if possible. Also there is this paper where they seemed to grow PC3 tumors just fine without matrigel:
I need some reference to the following question:
I need some rules of a thump for a retrospective multivariate survival analysis. We are looking at cases that got cancer of urinary bladder, prostate and kidney. All together there are 320 cases of urinary bladder, 1500 of prostate and 352 of kidney.
We have around 40 variables and want to do a multivariate survival analysis (approximately 300 deaths happened in all groups together) and a multivariate analysis of how those variables are related to the incidence of cancer (variables such as number of symptoms at their visit of GP,..).
For the multivariate analysis there is role of a thump to have 15-20 variables for each independent variable.
Does something like this exist for multivariate survival analysis for retrospective data (for example we have 320 cases of cancer in one group, how much deaths must occur to be able to calculate the multivariate survival analysis)?
Thank you very much for your help.
Are there any known foods and known food contents that can increase prostate enlargement related symptoms such as frequent calls and low urine flow most often with urine retention in the bladder?
I am trying to model 3D prostate CAD model for COMSOL multiphysics. I've heard that I can produce 3D image from CT image slices. Is there any way to get CT images slices? Also, it would be great to get some recommendation about software that converts 2D image slices to 3D CAD.
I need to isolate and culture prostate tumor cells from the clinical sample. The clinician will be providing the sample after prostectomy. So I ve to isolate tumor cells out of the sample which may contain normal cells too. Can anyone suggest me a method to differentiate tumor cells from the normal ones?
RWPE-1 and WPE-stem are two prostate cell lines which need K-SFM(Gibco 17005-042) as their culture medium,which is in the protocol provided by ATCC.Problem is that in China mainland,because the medium has BPE(bovine pituitary extract) as a component,it is prohibited to import from overseas.My colleagues told me KM(sciencell 2101) or defined K-SFM(Gibco 10744-019) could be used as equivalence.But I asked technical support of Gibco to assure it,they gave me a negative answer.Could anyone tell me is there substitute medium which also could be used to culture these two cells? Thanks!
I have downloaded CEL files of two datasets from GEO and I want to combine them. Each dataset has some benign prostate glands and some prostate cancer samples and they both have the same platform. first, I decided to compare their benign samples by R and if they didn’t show significant differences then I combine them. The result was totally unexpected! All q-values and p-values are significantly different and the numbers are very large. Now, I can’t understand why they are so different and I need to know whether it is necessary to compare the benign samples in order to combine them later? What criteria are important for combining two data sets?
I am beginning a literature review for the last semester for my degree and need some reliable statistics from England for the prevalence of prostate cancer and also statistics of how many men are undergoing PSA and/or prostate exams.
BRCA1 and BRCA2 are considered as tumour suppressor genes but mutations of these genes are selectively increase the risk of breast and ovarian cancers, compare to other type of tissue cancers.is there any reason for tissue specificity?
I am looking for a research article that has specifically written about histopathological effect of filed cancer in case of major cancer sites such as breast, lung, liver, prostate, colon, etc.
I am planning to use COBAS 4800 to test for HPV in prostate cancer.
What will be the best sample type for DNA extraction and the best protocol?
A 65 year old diabetic male.
Presented with first time Acute urine retention.
A urethral catheter was fixed for 2 weeks and tamsulosin starte
DRE revealed large prostate of benign feeling
LAS is 3.6 ng/ml
Ultrasound showed large prostate 200 gm
After removal of the cather he can void
Urodynamic showed hypocontractile bladder but he can void using abdominal pressure
PVR is 85 cc
I am doing IHC in prostate tissue and all the time I loose the nucleus in the cell. I follow the standard protocol. Kindly suggest me what different I should do?
The following is the protocol which I follow:
1. Xylene I 3mins
2. Xylene II 3mins
3. Xylene III 3mins
4. 100% EtoH 3mins
5. 100% EtoH 3mins
6. 90% EtoH 3mins
7. 75% EtoH 3mins
8. 50% EroH 3mins
9. Distilled water 5mins
10. Antigen Retrival (Soudium Citrate using vegetable steamer) 40mins
11. Room Temp 20mins
12. Distilled water 5mins
13. TBS 5mins
14. Peroxide Block 15mins
15. TBS 5mins
16. TBST 5mins
17. Blocking 13mins
18. 1oAb 1hr RT followed by 4oC overnight
19. TBS 5mins
20. TBST 5mins
21. Super Enhancer 20mins
22. TBS 3mins
23. TBST 3mins
24. 2oAb 30min
25. TBS 3mins
26. TBS 3mins
27. DAP 5mins
28. TBS 3mins
29. Distilled Water 3mins
30. Hematoxylin 15sec
31. Running Tap water 3mins
32. Blueing Solution 1min
33. Running Tap water 2mins
exercise and prostate cancer in animal model, and how long time for induction cacner in prostate of Rat
Are there studies to see if pre or post operative physiotherapy is more effective for post-prostatectomy patients?
I am looking for prostate adenocarcinoma images specifically. Ideally would be a large repository that is free and downloadable looking for these types of images:
Looking at the anatomy of the male urinary tract, there's the urinary bladder which gives off the prostatic urethra where urine passes through when the internal urethral sphincter relaxes. The prostate gland itself surrounds the prostatic urethra, and I would think the gland secretes into the urethra (hence how some urinary PSA gets into urine samples collected).
Do these prostatic secretions leak backwards through a contracted internal urinary sphincter and into the urine stored in the urinary bladder when a man is in a "resting state" (i.e. not urinating, which would relax the sphincter)?
I am Jakhongir F. Alidjanov, urologist from Uzbekistan, working on Research Project at the Justus-Liebig University of Giessen.
The main aim of the project is to investigate changes in metabolomic profile of the urine due to causative pathogens of urinary tract infections. Since being clinician, I am not so familiar with advanced microbiology. Therefore I would really appreciate if you could help me to clarify some advanced issues.
What pathogenic bacteria (E. coli, P. aeruginosa, E, faecalis, K. pneumoniae, S. saprophiticus etc.) do usually use as a nutrient source for living and multiplication in the urine? Below, I am posting some metabolites present in the urine which in my humble opinion could be appropriate to be investigated.
1. 1.3-propanediol (K. pneumoniae);
2. 4-Pyridoxic acid;
3. 6-hydroxylnicotinic acid (P. aeruginosa);
4. Acetate (highest in presence of Gram+);
5. Androsterone (may be reduced in stress urinary incontinence);
9. Formate (highest in presence of Gram-);
11. Glycerol (K. pneumoniae);
12. Glycolic acid;
13. Hippurate (highest in presence of Gram-);
14. Indoxyl sulphate (may be converted by uropathogens into indirubin and indigo – “purple bag syndrome”);
15. Lactate (highest in presence of Gram+);
18. L-fucose (may have an influence to virulence of some E. coli strains producing verotoxin);
25. Mandelic acid (antibacterial properties);
27. N-acetylneuraminic acid (found in cell membranes, may make a sense in diagnosing intracellular E. coli?);
28. Nicotinic acid (P. aeruginosa);
30. Succinate (highest in presence of Gram-);
32. Trimethylamine (E. coli);
33. Trimethylamine N-oxide (E. coli);
34. Urea (highest in presence of Gram-);
35. α-Aminoadipic acid;
Could you please look on them and give your suggestions regarding this issue? What else should we investigate?
Thank you all in advance for your responses.
I am a Medicine School student at Koç University (Currently a Junior.) I have an animal certificate regarding my eligiblity on conducting animal researches (CITI and Local Ethical Comitee.) I am now interested in prostate and testis cancer looking for a new research to be involved.
As I have understood,castration resistant prostate cancer is a rather clinical concept,which means prostate cancer progress to a non-responsive state to ADT after a session of ADT treatment.I am interested in how to understand CRPC from molecular and biological perspective.Although read articles in this field,unfortunately,I got no answer.So I ask this question here,and appreciate any expert can help me with my confusion.
I want to do classification of prostate E&H cancer tissue images
Need some Gleason graded dataset to train my algorithm
My topic of PhD research is "Computer Aided Detection of Prostate Cancer from MRI Images using Machine Intelligence Techniques". From where do I have to start prostate segmentation? Does registration has to be done before segmentation or is it optional? Are there any open source codes available for learning the existing methods of prostate segmentation?
which is the approximate incidence of pseudo hyperplastic prostate adenocarcinoma between carcinomas of its population or hospital?
I am trying to expand a prostate antigen specific T-cell population in NOD mice. I have tried to culture bone marrow derived DC's from NOD mice and not had much luck in getting functional mature DC's (CD11c low, MHCII high, CD86 pos). I also tried using irradiated splenocytes from a naive NOD mouse based off the methods section included in the PLOS ONE paper I have attached. The only way I strayed from this protocol was by using splenocytes, instead of a pure CD4 population. Please help me if you know of ANY other way to expand a specific population of T-cells
Uoflowmetry has limited role as an independent predictor of failure of medical management in patients with BPE.
I am developing a biomechanical model to estimate prostate deformations during brachytherapy. I am working with finite elements on a tetrahedral mesh derived from segmented images. I am trying to figure out currently how from the deformed mesh (result of finite element analysis) i will go back to the volumetric image (deformation of the initial image).
Any insight of somebody who have adressed similar tasks or any references would be very helpful.
Thank you in advance,
Unnecessary prostate biopsies can be avoided in patients with serum PSA values in gray-zone of 4-10 ng/ml by simply excluding prostatitis.
Dear RG community,
I need to obtain information of the prostate volume changes during the brachytherapy for my project. The software used in the collaborating clinic is Variseed for brachytherapy dosimetry. In the brochure of the software I see is possible to obtain a so-called "User-defined report for contiguous volume analysis" has anyone experience with the software and this feature?
Thank you very much in advance,
I need to induce chronic inflammation in prostate of adult rat using LPS via injection, but I don't want to use surgery.
Hello, I am investigating the evolution of prostate edema during brachytherapy. The key factors of the edema is the tissue trauma due to the needles insertion and the radiation deposition of the radioactive seeds. Are there any references to studies or experience regarding the needles insertion. I am trying to find standardised parameters for a generic model.
Hello, my question is about how I am able to add two or more meshes.
My goal is to export a mesh of a ct scan of the prostate. I do that using slicer3d to create a surface mesh from the label map. I continue by creating a volume mesh using gmsh derived from the surface mesh.
I want to set specific domain around the surgery needles (used during the brachytherapy procedure ) in the prostate mesh.
To do that I think to make a label mask for the needles position and create another mesh which will be consisted of a number of aligned parallelepipeds.
Now my question is if its possible to combine the volume mesh of the prostate with the volume mesh of the needles in order to obtain a mesh with multiple domains which I will use for FE analysis giving different conditions to the needles domain.
ps. By needle domain i refer in fact to a region of the prostate (around a needle) where edema is considered to be initialised.
Thank you in advance Konstantinos.
Hello, I am wondering if it is possible to give specific attributes in specific elements in COMSOL. In more detail I want to set some elements of the a real prostate mesh as liquid sources in an edema study. Is possible to give conditions to some elements by choice in COMSOL and if yes how?
Thank you in advance
Hello, I am trying to make a biomechanical model of the prostate gland using the COMSOL software and I am searching for proper biomechanical properties of the prostate for accurate modeling.
Could anyone help me in this task?
I need this characteristics:
1) tissue density
2) Young's modulus
3) Poisson's ratio
5) Biot-Willis coeficcient
Thank you in advance!!
Hello! I am trying to do a poroelasticity analysis for the prostate gland in the COMSOL framework using the poroelastic module. I need to set the poroelastic parameters of the prostate's material for the solution of the equations, however I cant find something relevant in the bibliography. Has anyone tried something similar for other soft tissues too? And if so what parameters have been used?
Thank you in advance for your answers.